Search

Your search keyword '"Freire T"' showing total 92 results
Start Over Author "Freire T" Search Limiters Full Text
92 results on '"Freire T"'

2. Nivel de conocimiento de Sífilis en adolescentes de dos Instituciones Educativas del distrito de Buenaventura

Author

María Eufemia Freire T, Alisson Viveros S, Ailing Stella Gallego P, and Paula Andrea Valdés M

Subjects
Sexual partner, medicine.medical_specialty, business.industry, Applied Mathematics, General Mathematics, Convenience sample, Signs and symptoms, Disease, medicine.disease, Eleventh, Family medicine, Sexual life, medicine, Syphilis, business
Abstract

Objetivo: Establecer el nivel de conocimiento de sífilis en adolescentes de dos Instituciones Educativas del distrito de Buenaventura de grados: noveno, décimo y once. Material y métodos: Estudio descriptivo de corte transversal en jóvenes entre 12 y 18 años; muestra a conveniencia de 420 estudiantes. Se aplicó una encuesta anónima dividida en 5 secciones. Se determinaron características sociodemográficas, hábitos, vida sexual y conocimiento sobre ITS. Resultados: 91% manifestó conocer sobre las ITS; 22,4% expresó conocer los signos y síntomas. 3,1& presentó diagnóstico de ITS; no reportaron casos de sífilis; 51,9% manifestó conocer la sífilis; el 10% expresó conocer signos y síntomas. 16% manifestó conocer mecanismo de prevención. 4% de los estudiantes tiene un buen nivel de conocimiento sobre sífilis. Sobre actividad sexual, 58,7% había iniciado vida sexual, 28% tuvo su primer encuentro a los 11 años o menos, siendo mayormente de género masculino. 33,8% habían tenido 1 pareja sexual, seguido de 23,8% con 6 o más parejas sexuales. El análisis de conocimiento sobre sífilis según institución educativa evidenció que el 8,9% y el 3% de los estudiantes tenía un nivel bueno de conocimiento, en la institución rural y urbana respectivamente. Al evaluar el conocimiento sobre ETS, se encontró que el 85% y 96% de estudiantes en zona rural y urbana respectivamente afirman tener conocimiento; sobre la sintomatología, 21% y 23 % es conocedor de esta, en área rural y urbana respectivamente. Conclusiones: Un alto porcentaje de estudiantes encuestados tienen un bajo nivel de conocimiento sobre sífilis; las mayores falencias se presentan en manifestaciones clínicas, la prevención y la cura de dicha enfermedad; aunque los adolescentes tienen múltiples fuentes de conocimiento, dicha información es deficiente, especialmente la transmitida por los padres y la pareja.

Published
2021
Full Text
View/download PDF

3. Adapting and Using Scrum in a Software Research and Development Laboratory

Author

LIMA, I. R., FREIRE, T. C., and COSTA, H. A. X.

Subjects
Scrum, Project management, Electronic computers. Computer science, QA75.5-76.95
Abstract

Agile software development has gained importance in the industry because of its approach on the issues of human agility and return on investment. This paper shows how Scrum agile software project management methodology has been deployed and adapted to the model of software project management of a research and development laboratory. As a result of this deployment, experiences and lessons learned in seven real projects developed by the authors are reported.

Published
2012

6. Análise do desempenho do modelo de Kostiakov na determinação da velocidade básica de infiltração em Plintossolo Pétrico concrecionário

Author

CORDEIRO, D. G., RAMOS, M. R, UHLMANN, A., CUSTÓDIO, D. P., SILVA, L. S. da, FREIRE, T. M., DANIEL PETTERSEN CUSTODIO, CNPASA, TALITA MAIA FREIRE, LORENA SANTOS DA SILVA., DIVONZIL GONCALVES CORDEIRO, CNPASA, MICHELE RIBEIRO RAMOS, UNIVERSIDADE ESTADUAL DO TOCANTINS, and ALEXANDRE UHLMANN, CNPASA

Subjects
Infiltration rate, Solo, Água do Solo, Soil water, Condutividade Hidráulica, Características físico-hídricas, Plintossolo, Infiltração
Abstract

Ainda são poucas as informações sobre as características físico-hídricas dos solos do Tocantins, apesar de ser um dado fundamental para recomendações de manejo. Os testes de infiltração de água foram realizados em um solo Plintossolo Pétrico concrecionário, uma classe de solos amplamente utilizada para fins agrícolas, mas ainda muito pouco conhecida. Este levantamento foi realizado em uma área degradada do Plintossolo Pétrico, em uma fazenda pecuária localizada no município de Brejinho de Nazaré/TO. Os dados foram coletados em solos localizados em um sistema integrado com sorgo e espécies lenhosas nativas. Antigamente, essa área era uma pastagem degradada. Para a medição da condutividade hidráulica, foi utilizado um conjunto de anéis triplos concêntricos acoplados a um sistema de abastecimento de água semiautomático (protótipo desenvolvido pelos pesquisadores da Embrapa Pesca e Aquicultura). Os dados brutos de campo foram ajustados ao modelo de Kostiakov para estimar a taxa de infiltração básica, que variou de 3,0 a 5,8 cm.h-1. Made available in DSpace on 2021-10-29T13:00:44Z (GMT). No. of bitstreams: 1 rbds-2021.pdf: 1860759 bytes, checksum: 71ee6838afd11d6e43bfede572f31ed5 (MD5) Previous issue date: 2021

Published
2021

8. Extratos vegetais do gênero capsicum com potencial atividade antifúngica contra Rhizoctonia solani e Sclerotium rolfsii

Author

FONSECA, A. S., FERNANDES, C. de F., VIEIRA JUNIOR, J. R., ROCHA, R. B., FREIRE, T. C., SANGI, S. C., BASTOS, J. S. F., ALVES, R. C., CLEBERSON DE FREITAS FERNANDES, CNPAT, JOSE ROBERTO VIEIRA JUNIOR, CPAF-RO, and RODRIGO BARROS ROCHA, CPAF-RO.

Subjects
Doenças fúngicas, Fitopatógenos
Abstract

Made available in DSpace on 2020-03-24T15:12:58Z (GMT). No. of bitstreams: 1 3667-Texto-do-artigo-8258-1-10-20200123.pdf: 1078825 bytes, checksum: 958643c9d5a0e1b48dc9177aa1eff93e (MD5) Previous issue date: 2020

Published
2020

9. Probing Hadronic Interactions with Measurements at Ultra-High Energies with the Pierre Auger Observatory

Author

nbsp;, D., Schmidt, A. undefined, Aab, P. undefined, Abreu, M. undefined, Aglietta, J. M. undefined, Albury, I. undefined, Allekotte, A. undefined, Almela, J. undefined, Alvarez-Muñiz, R. undefined, Alves Batista, G. A. undefined, Anastasi, L. undefined, Anchordoqui, B. undefined, Andrada, S. undefined, Andringa, C. undefined, Aramo, P. R. undefined, Araújo Ferreira, H. undefined, Asorey, P. undefined, Assis, G. undefined, Avila, A. M. undefined, Badescu, A. undefined, Bakalova, A. undefined, Balaceanu, F. undefined, Barbato, R. J. undefined, Barreira Luz, K. H. undefined, Becker, J. A. undefined, Bellido, C. undefined, Berat, M. E. undefined, Bertaina, X. undefined, Bertou, P. L. undefined, Bierman, T. undefined, Bister, J. undefined, Biteau, J. undefined, Blazek, C. undefined, Bleve, M. undefined, Boháčová, D. undefined, Boncioli, C. undefined, Bonifazi, L. undefined, Bonneau Arbeletche, N. undefined, Borodai, A. M. undefined, Botti, J. undefined, Brac, T. undefined, Bretz, F. L. undefined, Briechle, P. undefined, Buchholz, A. undefined, Bueno, S. undefined, Buitink, M. undefined, Buscemi, K. S. undefined, Caballero-Mora, L. undefined, Caccianiga, A. undefined, Cancio, F. undefined, Canfora, I. undefined, Caracas, J. M. undefined, Carceller, R. undefined, Caruso, A. undefined, Castellina, F. undefined, Catalani, G. undefined, Cataldi, L. undefined, Cazon, M. undefined, Cerda, J. A. undefined, Chinellato, K. undefined, Choi, J. undefined, Chudoba, L. undefined, Chytka, R. W. undefined, Clay, A. C. undefined, Cobos Cerutti, R. undefined, Colalillo, A. undefined, Coleman, M. R. undefined, Coluccia, R. undefined, Conceição, A. undefined, Condorelli, Consolati, G., undefined, F., Contreras, F. undefined, Convenga, C. E. undefined, Covault, S. undefined, Dasso, K. undefined, Daumiller, B. R. undefined, Dawson, J. A. undefined, Day, R. M. undefined, de Almeida, J. undefined, de Jesús, S. J. undefined, de Jong, G. undefined, De Mauro, J. R. T. undefined, de Mello Neto, I. undefined, De Mitri, J. undefined, de Oliveira, D. undefined, de Oliveira Franco, V. undefined, de Souza, E. undefined, De Vito, J. undefined, Debatin, M. undefined, del Río, O. undefined, Deligny, N. undefined, Dhital, A. undefined, Di Matteo, C. undefined, Dobrigkeit, J. C. undefined, D'Olivo, R. C. undefined, dos Anjos, M. T. undefined, Dova, J. undefined, Ebr, R. undefined, Engel, I. undefined, Epicoco, M. undefined, Erdmann, C. O. undefined, Escoba, A. undefined, Etchegoyen, H. undefined, Falcke, J. undefined, Farmer, G. undefined, Farrar, A. C. undefined, Fauth, N. undefined, Fazzi, F. undefined, Feldbusch, F. undefined, Fenu, B. undefined, Fick, J. M. undefined, Figueira, A. undefined, Filipčič, T. undefined, Fodran, M. M. undefined, Freire, T. undefined, Fujii, A. undefined, Fuster, C. undefined, Galea, C. undefined, Galelli, B. undefined, García, A. L. undefined, Garcia Vegas, H. undefined, Gemmeke, F. undefined, Gesualdi, A. undefined, Gherghel-Lascu, P. L. undefined, Ghia, U. undefined, Giaccari, M. undefined, Giammarchi, M. undefined, Giller, J. undefined, Glombitza, F. undefined, Gobbi, F. undefined, Gollan, G. undefined, Golup, M. undefined, Gómez Berisso, P. F. undefined, Gómez Vitale, J. P. undefined, Gongora, N. undefined, González, I. undefined, Goos, D. undefined, Góra, A. undefined, Gorgi, M. undefined, Gottowik, T. D. undefined, Grubb, F. undefined, Guarino, G. P. undefined, Guedes, E. undefined, Guido, S. undefined, Hahn, R. undefined, Halliday, M. R. undefined, Hampel, P. undefined, Hansen, D. undefined, Harari, V. M. undefined, Harvey, A. undefined, Haungs, T. undefined, Hebbeker, D. undefined, Heck, G. C. undefined, Hill, C. undefined, Hojvat, J. R. undefined, Hörandel, P. undefined, Horvath, M. undefined, Hrabovský, T. undefined, Huege, J. undefined, Hulsman, A. undefined, Insolia, P. G. undefined, Isar, J. A. undefined, Johnsen, J. undefined, Jurysek, A. undefined, Kääpä, K. H. undefined, Kampert, B. undefined, Keilhauer, J. undefined, Kemp, H. O. undefined, Klages, M. undefined, Kleifges, J. undefined, Kleinfeller, M. undefined, Köpke, G. undefined, Kukec Mezek, B. L. undefined, Lago, D. undefined, Lahurd, R. G. undefined, Lang, N. undefined, Langner, M. A. undefined, Leigui de Oliveira, V. undefined, Lenok, A. undefined, Letessier-Selvon, I. undefined, Lhenry-Yvon, D. undefined, Lo Presti, L. undefined, Lopes, R. undefined, López, R. undefined, Lorek, Q. undefined, Luce, A. undefined, Lucero, J. P. undefined, Lundquist, A. undefined, Machado Payeras, G. undefined, Mancarella, D. undefined, Mandat, B. C. undefined, Manning, J. undefined, Manshanden, P. undefined, Mantsc, S. undefined, Marafico, A. G. undefined, Mariazzi, I. C. undefined, Mariş, G. undefined, Marsella, D. undefined, Martello, H. undefined, Martinez, O. undefined, Martínez Bravo, M. undefined, Mastrodicasa, H. J. undefined, Mathes, J. undefined, Matthews, G. undefined, Matthiae, E. undefined, Mayotte, P. O. undefined, Mazu, G. undefined, Medina-Tanco, D. undefined, Melo, A. undefined, Menshikov, K. -D. undefined, Merenda, S. undefined, Michal, M. I. undefined, Micheletti, L. undefined, Miramonti, S. undefined, Mollerach, F. undefined, Montanet, C. undefined, Morello, M. undefined, Mostafá, A. L. undefined, Müller, M. A. undefined, Muller, K. undefined, Mulrey, R. undefined, Mussa, M. undefined, Muzio, W. M. undefined, Namasaka, L. undefined, Nellen, M. undefined, Niculescu-Oglinzanu, M. undefined, Niechciol, D. undefined, Nitz, D. undefined, Nosek, V. undefined, Novotny, L. undefined, Nožka, Nucita, A., undefined, L. A., Núñez, M. undefined, Palatka, J. undefined, Pallotta, P. undefined, Papenbreer, G. undefined, Parente, A. undefined, Parra, M. undefined, Pech, F. undefined, Pedreira, J. undefined, Pękala, R. undefined, Pelayo, J. undefined, Peña-Rodriguez, J. undefined, Perez Armand, M. undefined, Perlin, L. undefined, Perrone, S. undefined, Petrera, T. undefined, Pierog, M. undefined, Pimenta, V. undefined, Pirronello, M. undefined, Platino, B. undefined, Pont, M. undefined, Pothast, P. undefined, Privitera, M. undefined, Prouza, A. undefined, Puyleart, S. undefined, Querchfeld, J. undefined, Rautenberg, D. undefined, Ravignani, M. undefined, Reininghaus, J. undefined, Ridky, F. undefined, Riehn, M. undefined, Risse, P. undefined, Ristori, V. undefined, Rizi, W. undefined, Rodrigues de Carvalho, J. undefined, Rodriguez Rojo, M. J. undefined, Roncoroni, M. undefined, Roth, E. undefined, Roulet, A. C. undefined, Rovero, P. undefined, Ruehl, S. J. undefined, Saffi, A. undefined, Saftoiu, F. undefined, Salamida, H. undefined, Salazar, G. undefined, Salina, J. D. undefined, Sanabria Gomez, F. undefined, Sánchez, E. M. undefined, Santos, E. undefined, Santos, F. undefined, Sarazin, R. undefined, Sarmento, C. undefined, Sarmiento-Cano, R. undefined, Sato, P. undefined, Savina, C. M. undefined, Schäfer, V. undefined, Scherini, H. undefined, Schieler, M. undefined, Schimassek, M. undefined, Schimp, F. undefined, Schlüter, O. undefined, Scholten, P. undefined, Schovánek, F. G. undefined, Schröder, S. undefined, Schröder, J. undefined, Schulte, S. J. undefined, Sciutto, M. undefined, Scornavacche, R. C. undefined, Shellard, G. undefined, Sigl, G. undefined, Silli, O. undefined, Sima, R. undefined, Šmída, P. undefined, Sommers, J. F. undefined, Soriano, J. undefined, Souchard, R. undefined, Squartini, M. undefined, Stadelmaier, D. undefined, Stanca, S. undefined, Stanič, J. undefined, Stasielak, P. undefined, Stassi, A. undefined, Streich, M. undefined, Suárez-Durán, T. undefined, Sudholz, T. undefined, Suomijärvi, A. D. undefined, Supanitsky, J. undefined, Šupík, Z. undefined, Szadkowski, A. undefined, Taboada, A. undefined, Tapia, C. undefined, Timmermans, O. undefined, Tkachenko, P. undefined, Tobiska, C. J. undefined, Todero Peixoto, B. undefined, Tomé, A. undefined, Travaini, P. undefined, Travnicek, C. undefined, Trimarelli, M. undefined, Trini, M. undefined, Tueros, R. undefined, Ulrich, M. undefined, Unger, L. undefined, Vaclavek, M. undefined, Vacula, J. F. undefined, Valdés Galicia, L. undefined, Valore, E. undefined, Varela, V. undefined, Varma K. C., A. undefined, Vásquez-Ramírez, D. undefined, Veberič, C. undefined, Ventura, I. D. undefined, Vergara Quispe, V. undefined, Verzi, J. undefined, Vicha, J. undefined, Vink, S. undefined, Vorobiov, H. undefined, Wahlberg, A. A. undefined, Wat, M. undefined, Weber, A. undefined, Weindl, L. undefined, Wiencke, H. undefined, Wilczyński, T. undefined, Winchen, M. undefined, Wirtz, D. undefined, Wittkowski, B. undefined, Wundheiler, A. undefined, Yushkov, O. undefined, Zapparrata, E. undefined, Zas, D. undefined, Zavrtanik, M. undefined, Zavrtanik, L. undefined, Zehrer, A. undefined, and Zepeda

Published
2020

10. Atividade peroxidásica em feijoeiro comum (Phaseolus vulgaris L.) cv. Aporé na presença de ácido salicílico e Thanatephorus cucumeris

Author

FERNANDES, C. de F., VIEIRA JUNIOR, J. R., AGUIAR, P. das N. de, SOUZA, M. F., FREIRE, T. C., FONSECA, A. S. da, ALVES, R. de C., CLEBERSON DE FREITAS FERNANDES, CNPAT, and JOSE ROBERTO VIEIRA JUNIOR, CNPAT.

Subjects
Thanatephorus Cucumeris, Feijão
Abstract

Made available in DSpace on 2020-03-24T15:12:37Z (GMT). No. of bitstreams: 1 document10.pdf: 890672 bytes, checksum: a67ee1b52859e4dff0bd00f627dd2c8b (MD5) Previous issue date: 2020

Published
2020

11. The Radio Detection of Inclined Showers at the Pierre Auger Observatory

Author

S. de Jong, A. &nbsp, Aab, P. undefined, Abreu, M. undefined, Aglietta, J. M. undefined, Albury, I. undefined, Allekotte, A. undefined, Almela, J. undefined, Alvarez-Muñiz, R. undefined, Alves Batista, G. A. undefined, Anastasi, L. undefined, Anchordoqui, B. undefined, Andrada, S. undefined, Andringa, C. undefined, Aramo, P. R. undefined, Araújo Ferreira, H. undefined, Asorey, P. undefined, Assis, G. undefined, Avila, A. M. undefined, Badescu, A. undefined, Bakalova, A. undefined, Balaceanu, F. undefined, Barbato, R. J. undefined, Barreira Luz, K. H. undefined, Becker, J. A. undefined, Bellido, C. undefined, Berat, M. E. undefined, Bertaina, X. undefined, Bertou, P. L. undefined, Bierman, T. undefined, Bister, J. undefined, Biteau, J. undefined, Blazek, C. undefined, Bleve, M. undefined, Boháčová, D. undefined, Boncioli, C. undefined, Bonifazi, L. undefined, Bonneau Arbeletche, N. undefined, Borodai, A. M. undefined, Botti, J. undefined, Brac, T. undefined, Bretz, F. L. undefined, Briechle, P. undefined, Buchholz, A. undefined, Bueno, S. undefined, Buitink, M. undefined, Buscemi, K. S. undefined, Caballero-Mora, L. undefined, Caccianiga, A. undefined, Cancio, F. undefined, Canfora, I. undefined, Caracas, J. M. undefined, Carceller, R. undefined, Caruso, A. undefined, Castellina, F. undefined, Catalani, G. undefined, Cataldi, L. undefined, Cazon, M. undefined, Cerda, J. A. undefined, Chinellato, K. undefined, Choi, J. undefined, Chudoba, L. undefined, Chytka, R. W. undefined, Clay, A. C. undefined, Cobos Cerutti, R. undefined, Colalillo, A. undefined, Coleman, M. R. undefined, Coluccia, R. undefined, Conceição, A. undefined, Condorelli, Consolati, G., undefined, F., Contreras, F. undefined, Convenga, C. E. undefined, Covault, S. undefined, Dasso, K. undefined, Daumiller, B. R. undefined, Dawson, J. A. undefined, Day, R. M. undefined, de Almeida, J. undefined, de Jesús, G. undefined, De Mauro, J. R. T. undefined, de Mello Neto, I. undefined, De Mitri, J. undefined, de Oliveira, D. undefined, de Oliveira Franco, V. undefined, de Souza, E. undefined, De Vito, J. undefined, Debatin, M. undefined, del Río, O. undefined, Deligny, N. undefined, Dhital, A. undefined, Di Matteo, C. undefined, Dobrigkeit, J. C. undefined, D'Olivo, R. C. undefined, dos Anjos, M. T. undefined, Dova, J. undefined, Ebr, R. undefined, Engel, I. undefined, Epicoco, M. undefined, Erdmann, C. O. undefined, Escoba, A. undefined, Etchegoyen, H. undefined, Falcke, J. undefined, Farmer, G. undefined, Farrar, A. C. undefined, Fauth, N. undefined, Fazzi, F. undefined, Feldbusch, F. undefined, Fenu, B. undefined, Fick, J. M. undefined, Figueira, A. undefined, Filipčič, T. undefined, Fodran, M. M. undefined, Freire, T. undefined, Fujii, A. undefined, Fuster, C. undefined, Galea, C. undefined, Galelli, B. undefined, García, A. L. undefined, Garcia Vegas, H. undefined, Gemmeke, F. undefined, Gesualdi, A. undefined, Gherghel-Lascu, P. L. undefined, Ghia, U. undefined, Giaccari, M. undefined, Giammarchi, M. undefined, Giller, J. undefined, Glombitza, F. undefined, Gobbi, F. undefined, Gollan, G. undefined, Golup, M. undefined, Gómez Berisso, P. F. undefined, Gómez Vitale, J. P. undefined, Gongora, N. undefined, González, I. undefined, Goos, D. undefined, Góra, A. undefined, Gorgi, M. undefined, Gottowik, T. D. undefined, Grubb, F. undefined, Guarino, G. P. undefined, Guedes, E. undefined, Guido, S. undefined, Hahn, R. undefined, Halliday, M. R. undefined, Hampel, P. undefined, Hansen, D. undefined, Harari, V. M. undefined, Harvey, A. undefined, Haungs, T. undefined, Hebbeker, D. undefined, Heck, G. C. undefined, Hill, C. undefined, Hojvat, J. R. undefined, Hörandel, P. undefined, Horvath, M. undefined, Hrabovský, T. undefined, Huege, J. undefined, Hulsman, A. undefined, Insolia, P. G. undefined, Isar, J. A. undefined, Johnsen, J. undefined, Jurysek, A. undefined, Kääpä, K. H. undefined, Kampert, B. undefined, Keilhauer, J. undefined, Kemp, H. O. undefined, Klages, M. undefined, Kleifges, J. undefined, Kleinfeller, M. undefined, Köpke, G. undefined, Kukec Mezek, B. L. undefined, Lago, D. undefined, Lahurd, R. G. undefined, Lang, N. undefined, Langner, M. A. undefined, Leigui de Oliveira, V. undefined, Lenok, A. undefined, Letessier-Selvon, I. undefined, Lhenry-Yvon, D. undefined, Lo Presti, L. undefined, Lopes, R. undefined, López, R. undefined, Lorek, Q. undefined, Luce, A. undefined, Lucero, J. P. undefined, Lundquist, A. undefined, Machado Payeras, G. undefined, Mancarella, D. undefined, Mandat, B. C. undefined, Manning, J. undefined, Manshanden, P. undefined, Mantsc, S. undefined, Marafico, A. G. undefined, Mariazzi, I. C. undefined, Mariş, G. undefined, Marsella, D. undefined, Martello, H. undefined, Martinez, O. undefined, Martínez Bravo, M. undefined, Mastrodicasa, H. J. undefined, Mathes, J. undefined, Matthews, G. undefined, Matthiae, E. undefined, Mayotte, P. O. undefined, Mazu, G. undefined, Medina-Tanco, D. undefined, Melo, A. undefined, Menshikov, K. -D. undefined, Merenda, S. undefined, Michal, M. I. undefined, Micheletti, L. undefined, Miramonti, S. undefined, Mollerach, F. undefined, Montanet, C. undefined, Morello, M. undefined, Mostafá, A. L. undefined, Müller, M. A. undefined, Muller, K. undefined, Mulrey, R. undefined, Mussa, M. undefined, Muzio, W. M. undefined, Namasaka, L. undefined, Nellen, M. undefined, Niculescu-Oglinzanu, M. undefined, Niechciol, D. undefined, Nitz, D. undefined, Nosek, V. undefined, Novotny, L. undefined, Nožka, Nucita, A., undefined, L. A., Núñez, M. undefined, Palatka, J. undefined, Pallotta, P. undefined, Papenbreer, G. undefined, Parente, A. undefined, Parra, M. undefined, Pech, F. undefined, Pedreira, J. undefined, Pękala, R. undefined, Pelayo, J. undefined, Peña-Rodriguez, J. undefined, Perez Armand, M. undefined, Perlin, L. undefined, Perrone, S. undefined, Petrera, T. undefined, Pierog, M. undefined, Pimenta, V. undefined, Pirronello, M. undefined, Platino, B. undefined, Pont, M. undefined, Pothast, P. undefined, Privitera, M. undefined, Prouza, A. undefined, Puyleart, S. undefined, Querchfeld, J. undefined, Rautenberg, D. undefined, Ravignani, M. undefined, Reininghaus, J. undefined, Ridky, F. undefined, Riehn, M. undefined, Risse, P. undefined, Ristori, V. undefined, Rizi, W. undefined, Rodrigues de Carvalho, J. undefined, Rodriguez Rojo, M. J. undefined, Roncoroni, M. undefined, Roth, E. undefined, Roulet, A. C. undefined, Rovero, P. undefined, Ruehl, S. J. undefined, Saffi, A. undefined, Saftoiu, F. undefined, Salamida, H. undefined, Salazar, G. undefined, Salina, J. D. undefined, Sanabria Gomez, F. undefined, Sánchez, E. M. undefined, Santos, E. undefined, Santos, F. undefined, Sarazin, R. undefined, Sarmento, C. undefined, Sarmiento-Cano, R. undefined, Sato, P. undefined, Savina, C. M. undefined, Schäfer, V. undefined, Scherini, H. undefined, Schieler, M. undefined, Schimassek, M. undefined, Schimp, F. undefined, Schlüter, D. undefined, Schmidt, O. undefined, Scholten, P. undefined, Schovánek, F. G. undefined, Schröder, S. undefined, Schröder, J. undefined, Schulte, S. J. undefined, Sciutto, M. undefined, Scornavacche, R. C. undefined, Shellard, G. undefined, Sigl, G. undefined, Silli, O. undefined, Sima, R. undefined, Šmída, P. undefined, Sommers, J. F. undefined, Soriano, J. undefined, Souchard, R. undefined, Squartini, M. undefined, Stadelmaier, D. undefined, Stanca, S. undefined, Stanič, J. undefined, Stasielak, P. undefined, Stassi, A. undefined, Streich, M. undefined, Suárez-Durán, T. undefined, Sudholz, T. undefined, Suomijärvi, A. D. undefined, Supanitsky, J. undefined, Šupík, Z. undefined, Szadkowski, A. undefined, Taboada, A. undefined, Tapia, C. undefined, Timmermans, O. undefined, Tkachenko, P. undefined, Tobiska, C. J. undefined, Todero Peixoto, B. undefined, Tomé, A. undefined, Travaini, P. undefined, Travnicek, C. undefined, Trimarelli, M. undefined, Trini, M. undefined, Tueros, R. undefined, Ulrich, M. undefined, Unger, L. undefined, Vaclavek, M. undefined, Vacula, J. F. undefined, Valdés Galicia, L. undefined, Valore, E. undefined, Varela, V. undefined, Varma K. C., A. undefined, Vásquez-Ramírez, D. undefined, Veberič, C. undefined, Ventura, I. D. undefined, Vergara Quispe, V. undefined, Verzi, J. undefined, Vicha, J. undefined, Vink, S. undefined, Vorobiov, H. undefined, Wahlberg, A. A. undefined, Wat, M. undefined, Weber, A. undefined, Weindl, L. undefined, Wiencke, H. undefined, Wilczyński, T. undefined, Winchen, M. undefined, Wirtz, D. undefined, Wittkowski, B. undefined, Wundheiler, A. undefined, Yushkov, O. undefined, Zapparrata, E. undefined, Zas, D. undefined, Zavrtanik, M. undefined, Zavrtanik, L. undefined, Zehrer, A. undefined, and Zepeda

Published
2020

12. Multi-Messenger Studies with the Pierre Auger Observatory

Author

nbsp;, L., Zehrer, A. undefined, Aab, P. undefined, Abreu, M. undefined, Aglietta, J. M. undefined, Albury, I. undefined, Allekotte, A. undefined, Almela, J. undefined, Alvarez-Muniz, R. undefined, Alves Batista, G. A. undefined, Anastasi, L. undefined, Anchordoqui, B. undefined, Andrada, S. undefined, Andringa, C. undefined, Aramo, P. R. undefined, Araújo Ferreira, H. undefined, Asorey, P. undefined, Assis, G. undefined, Avila, A. M. undefined, Badescu, A. undefined, Bakalova, A. undefined, Balaceanu, F. undefined, Barbato, R. J. undefined, Barreira Luz, K. H. undefined, Becker, J. A. undefined, Bellido, C. undefined, Berat, M. E. undefined, Bertaina, X. undefined, Bertou, P. L. undefined, Bierman, T. undefined, Bister, J. undefined, Biteau, J. undefined, Blazek, C. undefined, Bleve, M. undefined, Bohácová, D. undefined, Boncioli, C. undefined, Bonifazi, L. undefined, Bonneau Arbeletche, N. undefined, Borodai, A. M. undefined, Botti, J. undefined, Brac, T. undefined, Bretz, F. L. undefined, Briechle, P. undefined, Buchholz, A. undefined, Bueno, S. undefined, Buitink, M. undefined, Buscemi, K. S. undefined, Caballero-Mora, L. undefined, Caccianiga, A. undefined, Cancio, F. undefined, Canfora, I. undefined, Caracas, J. M. undefined, Carceller, R. undefined, Caruso, A. undefined, Castellina, F. undefined, Catalani, G. undefined, Cataldi, L. undefined, Cazon, M. undefined, Cerda, J. A. undefined, Chinellato, K. undefined, Choi, J. undefined, Chudoba, L. undefined, Chytka, R. W. undefined, Clay, A. C. undefined, Cobos Cerutti, R. undefined, Colalillo, A. undefined, Coleman, M. R. undefined, Coluccia, R. undefined, Conceicao, A. undefined, Condorelli, Consolati, G., undefined, F., Contreras, F. undefined, Convenga, C. E. undefined, Covault, S. undefined, Dasso, K. undefined, Daumiller, B. R. undefined, Dawson, J. A. undefined, Day, R. M. undefined, de Almeida, J. undefined, de Jesús, S. J. undefined, de Jong, G. undefined, De Mauro, J. R. T. undefined, de Mello Neto, I. undefined, De Mitri, J. undefined, de Oliveira, D. undefined, de Oliveira Franco, V. undefined, de Souza, E. undefined, De Vito, J. undefined, Debatin, M. undefined, del Río, O. undefined, Deligny, N. undefined, Dhital, A. undefined, Di Matteo, C. undefined, Dobrigkeit, J. C. undefined, D'Olivo, R. C. undefined, dos Anjos, M. T. undefined, Dova, J. undefined, Ebr, R. undefined, Engel, I. undefined, Epicoco, M. undefined, Erdmann, C. O. undefined, Escoba, A. undefined, Etchegoyen, H. undefined, Falcke, J. undefined, Farmer, G. undefined, Farrar, A. C. undefined, Fauth, N. undefined, Fazzi, F. undefined, Feldbusch, F. undefined, Fenu, B. undefined, Fick, J. M. undefined, Figueira, A. undefined, Filipcic, T. undefined, Fodran, M. M. undefined, Freire, T. undefined, Fujii, A. undefined, Fuster, C. undefined, Galea, C. undefined, Galelli, B. undefined, García, A. L. undefined, Garcia Vegas, H. undefined, Gemmeke, F. undefined, Gesualdi, A. undefined, Gherghel-Lascu, P. L. undefined, Ghia, U. undefined, Giaccari, M. undefined, Giammarchi, M. undefined, Giller, J. undefined, Glombitza, F. undefined, Gobbi, F. undefined, Gollan, G. undefined, Golup, M. undefined, Gómez Berisso, P. F. undefined, Gómez Vitale, J. P. undefined, Gongora, N. undefined, González, I. undefined, Goos, D. undefined, Góra, A. undefined, Gorgi, M. undefined, Gottowik, T. D. undefined, Grubb, F. undefined, Guarino, G. P. undefined, Guedes, E. undefined, Guido, S. undefined, Hahn, R. undefined, Halliday, M. R. undefined, Hampel, P. undefined, Hansen, D. undefined, Harari, V. M. undefined, Harvey, A. undefined, Haungs, T. undefined, Hebbeker, D. undefined, Heck, G. C. undefined, Hill, C. undefined, Hojvat, J. R. undefined, Horandel, P. undefined, Horvath, M. undefined, Hrabovsky, T. undefined, Huege, J. undefined, Hulsman, A. undefined, Insolia, P. G. undefined, Isar, J. A. undefined, Johnsen, J. undefined, Jurysek, A. undefined, Kaapa, K. H. undefined, Kampert, B. undefined, Keilhauer, J. undefined, Kemp, H. O. undefined, Klages, M. undefined, Kleifges, J. undefined, Kleinfeller, M. undefined, Köpke, G. undefined, Kukec Mezek, B. L. undefined, Lago, D. undefined, Lahurd, R. G. undefined, Lang, N. undefined, Langner, M. A. undefined, Leigui de Oliveira, V. undefined, Lenok, A. undefined, Letessier-Selvon, I. undefined, Lhenry-Yvon, D. undefined, Lo Presti, L. undefined, Lopes, R. undefined, López, R. undefined, Lorek, Q. undefined, Luce, A. undefined, Lucero, J. P. undefined, Lundquist, A. undefined, Machado Payeras, G. undefined, Mancarella, D. undefined, Mandat, B. C. undefined, Manning, J. undefined, Manshanden, P. undefined, Mantsc, S. undefined, Marafico, A. G. undefined, Mariazzi, I. C. undefined, Mariş, G. undefined, Marsella, D. undefined, Martello, H. undefined, Martinez, O. undefined, Martínez Bravo, M. undefined, Mastrodicasa, H. J. undefined, Mathes, J. undefined, Matthews, G. undefined, Matthiae, E. undefined, Mayotte, P. O. undefined, Mazu, G. undefined, Medina-Tanco, D. undefined, Melo, A. undefined, Menshikov, K. -D. undefined, Merenda, S. undefined, Michal, M. I. undefined, Micheletti, L. undefined, Miramonti, S. undefined, Mollerach, F. undefined, Montanet, C. undefined, Morello, M. undefined, Mostafá, A. L. undefined, Muller, M. A. undefined, Muller, K. undefined, Mulrey, R. undefined, Mussa, M. undefined, Muzio, W. M. undefined, Namasaka, L. undefined, Nellen, M. undefined, Niculescu-Oglinzanu, M. undefined, Niechciol, D. undefined, Nitz, D. undefined, Nosek, V. undefined, Novotny, L. undefined, Nožka, Nucita, A., undefined, L. A., Nunez, M. undefined, Palatka, J. undefined, Pallotta, P. undefined, Papenbreer, G. undefined, Parente, A. undefined, Parra, M. undefined, Pech, F. undefined, Pedreira, J. undefined, Pękala, R. undefined, Pelayo, J. undefined, Pena-Rodriguez, J. undefined, Perez Armand, M. undefined, Perlin, L. undefined, Perrone, S. undefined, Petrera, T. undefined, Pierog, M. undefined, Pimenta, V. undefined, Pirronello, M. undefined, Platino, B. undefined, Pont, M. undefined, Pothast, P. undefined, Privitera, M. undefined, Prouza, A. undefined, Puyleart, S. undefined, Querchfeld, J. undefined, Rautenberg, D. undefined, Ravignani, M. undefined, Reininghaus, J. undefined, Ridky, F. undefined, Riehn, M. undefined, Risse, P. undefined, Ristori, V. undefined, Rizi, W. undefined, Rodrigues de Carvalho, J. undefined, Rodriguez Rojo, M. J. undefined, Roncoroni, M. undefined, Roth, E. undefined, Roulet, A. C. undefined, Rovero, P. undefined, Ruehl, S. J. undefined, Saffi, A. undefined, Saftoiu, F. undefined, Salamida, H. undefined, Salazar, G. undefined, Salina, J. D. undefined, Sanabria Gomez, F. undefined, Sánchez, E. M. undefined, Santos, E. undefined, Santos, F. undefined, Sarazin, R. undefined, Sarmento, C. undefined, Sarmiento-Cano, R. undefined, Sato, P. undefined, Savina, C. M. undefined, Schafer, V. undefined, Scherini, H. undefined, Schieler, M. undefined, Schimassek, M. undefined, Schimp, F. undefined, Schluter, D. undefined, Schmidt, O. undefined, Scholten, P. undefined, Schovánek, F. G. undefined, Schroder, S. undefined, Schroder, J. undefined, Schulte, S. J. undefined, Sciutto, M. undefined, Scornavacche, R. C. undefined, Shellard, G. undefined, Sigl, G. undefined, Silli, O. undefined, Sima, R. undefined, Smída, P. undefined, Sommers, J. F. undefined, Soriano, J. undefined, Souchard, R. undefined, Squartini, M. undefined, Stadelmaier, D. undefined, Stanca, S. undefined, Stanic, J. undefined, Stasielak, P. undefined, Stassi, A. undefined, Streich, M. undefined, Suárez-Durán, T. undefined, Sudholz, T. undefined, Suomijärvi, A. D. undefined, Supanitsky, J. undefined, Supík, Z. undefined, Szadkowski, A. undefined, Taboada, A. undefined, Tapia, C. undefined, Timmermans, O. undefined, Tkachenko, P. undefined, Tobiska, C. J. undefined, Todero Peixoto, B. undefined, Tomé, A. undefined, Travaini, P. undefined, Travnicek, C. undefined, Trimarelli, M. undefined, Trini, M. undefined, Tueros, R. undefined, Ulrich, M. undefined, Unger, L. undefined, Vaclavek, M. undefined, Vacula, J. F. undefined, Valdés Galicia, L. undefined, Valore, E. undefined, Varela, V. undefined, Varma K. C., A. undefined, Vásquez-Ramírez, D. undefined, Veberic, C. undefined, Ventura, I. D. undefined, Vergara Quispe, V. undefined, Verzi, J. undefined, Vicha, J. undefined, Vink, S. undefined, Vorobiov, H. undefined, Wahlberg, A. A. undefined, Wat, M. undefined, Weber, A. undefined, Weindl, L. undefined, Wiencke, H. undefined, Wilczynski, T. undefined, Winchen, M. undefined, Wirtz, D. undefined, Wittkowski, B. undefined, Wundheiler, A. undefined, Yushkov, O. undefined, Zapparrata, E. undefined, Zas, D. undefined, Zavrtanik, M. undefined, Zavrtanik, A. undefined, and Zepeda

Published
2020

13. Potencial de controle de espécies de Piper sobre fungos fitopatogênicos

Author

BASTOS, J. S. F., FERNANDES, C. de F., VIEIRA JUNIOR, J. R., FONSECA, A. S., FREIRE, T. C., SANGI, S. C., OGRODOWCZYK, L., OLIVEIRA, K. C. C., ROCHA, R. B., BARBIERI, F. da S., Jessica Silva Felix Bastos, Universidade Federal de Rondônia, Cléberson de Freitas Fernandes, Universidade Federal do Ceará, José Roberto Vieira Junior, Universidade Federal de Rondônia, Aline Souza Fonseca, Universidade Federal de Rondônia, Tamiris Chaves Freire, Universidade Federal de Rondônia, Simone Carvalho Sangi, Universidade Federal de Rondônia, Liliani Ogrodowczyk, Universidade Federal de Rondônia, Karen Cristina Chaves Oliveira, Faculdades Integradas Aparício Carvalho, RODRIGO BARROS ROCHA, CPAF-RO, and Fábio da Silva Barbieri, Universidade Federal Rural do Rio de Janeiro.

Subjects
Piper
Abstract

Made available in DSpace on 2019-12-17T00:36:31Z (GMT). No. of bitstreams: 1 Piper.pdf: 1242502 bytes, checksum: 7f464c6d9df9abf240b5837107504dab (MD5) Previous issue date: 2018

Published
2018

14. Extratos de Piper no controle alternativo de fitonematoides do gênero Meloidogyne em Coffea canephora

Author

SANGI, S. C., VIEIRA JUNIOR, J. R., FERNANDES, C. de F., BASTOS, J. S. F., FONSECA, A. S., FREIRE, T. C., OGRODOWCZYK, L., NUNES, J. D. K., OLIVEIRA, K. C. C., ROCHA, R. B., BARBIERI, F. da S., and JOSE ROBERTO VIEIRA JUNIOR, CPAF-RO.

Subjects
Café
Abstract

Made available in DSpace on 2019-10-02T00:42:57Z (GMT). No. of bitstreams: 1 artigo2018.pdf: 1285817 bytes, checksum: e2fbd72c6d23fd705b942b21508c1adc (MD5) Previous issue date: 2018

Published
2018

15. Extratos de espécies de capsicum no controle in vitro de patógenos de importância agrícola

Author

VIEIRA JUNIOR, J. R., FERNANDES, C. de F., SANGI, S. C., FREIRE, T. C., FONSECA, A. S. da, JOSE ROBERTO VIEIRA JUNIOR, CPAF-Rondonia, CLEBERSON DE FREITAS FERNANDES, CNPAT, Simone Carvalho Sangi, Mestranda, UNIR, Tamiris Chaves Freire, Doutoranda, UNIR, and Aline Souza da Fonseca, Doutoranda, UNIR.

Subjects
Patogenicidade, Inibição, Doenças fúngicas, Controle Biológico, Método alternativo
Abstract

Nos últimos 30 anos tem-se observado, por parte da sociedade, uma maior preocupação com o uso de agrotóxicos na agricultura. Nesse sentido, a pesquisa tem buscado incansavelmente produtos alternativos, que sejam mais eficientes, baratos e ambientalmente seguros. Dentre as alternativas buscadas, o uso de extratos de plantas tem se mostrado promissor. Nesse sentido, o presente trabalho teve como objetivo testar 48 extratos aquosos e alcoólicos de 18 variedades de pimentas do gênero Capsicum obtidos a partir de sementes, folhas e frutos pelo método de maceração para o controle na inibição de germinação de Hemileia vastatrix e crescimento micelial dos fungos Colletotrichum lindemuthianum, Alternaria sp., Fusarium sp. Colletotrichum gloeosporioides e Phomopsis. Destes, o extrato aquoso, obtido de sementes de Capsicum chinense foi o mais eficiente, controlando todos os fitopatógenos desafiantes, mostrando que esses extratos possuem compostos bioativos possíveis de controlar fungos fitopatogênicos. PALAVRAS-CHAVE: Doenças fúngicas, Inibição, Método alternativo. Made available in DSpace on 2018-03-06T00:34:06Z (GMT). No. of bitstreams: 1 ExtratoVieiraJUNIOR.pdf: 664958 bytes, checksum: 2688ab0fe9e0c9cac5553c2babe319f7 (MD5) Previous issue date: 2018-03-05

Published
2017

16. Natural or artificial? Multi-analytical study of a scagliola from Estoi palace simulating imperial red porphyry

Author

Freire, T., Santos Silva, A., Veiga, M. R., Brito, J., Schlütter, F., SIM, and NAO

Subjects
Microscopy, Multiphase gypsum plasters, Scagliola, Gypsum technology, Multi-analytical characterization
Abstract

In this paper the characterization of a gypsum plaster sample from the end of the 19th century simulating imperial red porphyry using a multi-analytical approach is presented and discussed. The results of X-ray diffraction (XRD), thermogravimetric and differential thermal analysis (TGA-DTA), physical and mechanical properties are summarized. In order to have further insight into the microstructure, polarized light microscopy (PLM), scanning electron microscopy coupled with energy dispersive X-ray spectrometer (SEMEDS), and micro Raman spectroscopy analyzes were also made. They helped to clarify the main issues raised by the other complementary analytical techniques and allowed the establishment of interrelations between the different properties, providing important information about the materials, the skills, and the technological development involved in the art of imitating noble stones with gypsum pastes. This study also contributes to our knowledge concerning the preservation of these types of elements that are important in the context of European decorative arts and rarely reported in the literature. 1281-1303pp M.T.F.’s PhD research study was supported by the scholarship SFRH/BD/40128/2007 from Fundação para a Ciência e Tecnologia (FCT). The authors would like to thank the technicians Paula Menezes from the Materials Department of LNEC, and Ana Duarte, Dora Santos, and Bento Sabala from the Buildings Department, for their support on the execution of the experimental work; LNEC for the support within the project 0204/11/17692 Materials with Historical Interest—Durability and Characterization, and the research center CERIS from Instituto Superior Técnico, University of Lisbon. Volume 22, Issue 6 DM/NMM Microscopy and Microanalysis

Published
2016

17. The history of Portuguese interior plaster coatings: A mineralogical survey using XRD

Author

Freire, T., Santos Silva, A., Veiga, M. R., and Brito, J.

Subjects
Xrd analysis, Portuguese heritage, Mineralogical composition, Lime plaster, Gypsum plaster, Interior coatings
Abstract

Submitted by António Silva (ssilva@lnec.pt) on 2015-02-13T17:43:55Z No. of bitstreams: 1 Freire et al_2014_Abstract.pdf: 40100 bytes, checksum: 8374776fcc5c83e3ef61d890d8c92706 (MD5) Approved for entry into archive by Ana Duarte (aduarte@lnec.pt) on 2015-02-13T17:53:17Z (GMT) No. of bitstreams: 1 Freire et al_2014_Abstract.pdf: 40100 bytes, checksum: 8374776fcc5c83e3ef61d890d8c92706 (MD5) Made available in DSpace on 2015-02-13T17:53:17Z (GMT). No. of bitstreams: 1 Freire et al_2014_Abstract.pdf: 40100 bytes, checksum: 8374776fcc5c83e3ef61d890d8c92706 (MD5) Previous issue date: 2014 Made available in DSpace on 2017-04-12T13:42:55Z (GMT). No. of bitstreams: 2 license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Freire et al_2014_Abstract.pdf: 40100 bytes, checksum: 8374776fcc5c83e3ef61d890d8c92706 (MD5) Previous issue date: 2014 19p Teresa Freire’s Ph.D. research study was supported by scholarship SFRH/BD/40128/2007 from the Fundação para a Ciência e Tecnologia (FCT).We would like to thank Susana Couto and Paula Menezes, from the Materials Department of LNEC, for their support in the execution of the experimental work, LNEC for support within project 0204/11/17692, Materials with Historical Interest—Durability and Characterization, and the ICIST research centre of the Instituto Superior Técnico, Technical University of Lisbon. DM/NMM Archaeometry

Published
2014

18. Happiness through leisure

Author

Nawijn, J, Veenhoven, R., Freire, T., Sociology, Academy for Tourism, and Research group for Tourism Impacts on Society

Subjects
Value (ethics), Casual, media_common.quotation_subject, Happiness, Context (language use), Big Five personality traits, Psychology, Set (psychology), Social psychology, media_common
Abstract

In this chapter, we start with a description of happiness, the nature of happiness, the value of happiness, and the determinants of happiness. In that context, we discuss what leisure can contribute to happiness. Although happiness is partially set through inherited personality traits, there is potential for improving one's sense of happiness through leisure. Research to date focused mainly on casual leisure and leisure travel. These kinds of leisure have mostly a small positive effect on happiness. The benefits are often temporary and in the moment. We propose more longitudinal research that addresses serious leisure and project-based leisure, which may have more sustained effects on happiness than casual leisure and leisure travel.

Published
2013

19. Characterization of decorative Portuguese gypsum plasters from the 19th and 20th centuries: the case of the Bolsa Palace in Oporto

Author

Freire, T., Santos Silva, A., Veiga, M. R., Brito, J., and Jan Valék, John J. Hughes and Caspar J.W.P. Groot

Subjects
Microscopy, Characterisation, Xrd, Tga-dta, Gypsum plasters, Capillary absorption
Abstract

The use of gypsum plaster for the interior coating of walls and ceilings in the Portuguese architecture was particularly expressive in the period between the XVIII and the XX century. However, information about this important heritage is almost nonexistent, which is leading to a rapid loss of important patrimony. In this paper the results of the characterisation of five gypsum plaster samples from the second half of the XIX century belonging to the Arabian Room of the Bolsa Palace, located in Oporto, North of Portugal, are presented and discussed. XRD and TGA-DTA techniques were used to establish the mineralogical composition, and the relative proportions of the binders. Optical microscopy and FESEM-EDS observations were performed both in fractured and polished surfaces in order to determine the stratigraphy and the composition of individual layers. The results of this characterisation work showed that the plasters used were mainly composed of gypsum and hydrated lime in different proportions – a feature that was correlated with the application techniques of the decorative elements analyzed - and allowed the determining of the restoration interventions they had already been submitted to. Some physical properties like the dynamic modulus of elasticity and capillary absorption were also determined, and a correlation between the results obtained was established with previous studies performed by the authors. Teresa Freire’s PhD research is being supported by the scholarship SFRH/BD/40128/2007 from Fundação para a Ciência e Tecnologia (FCT). The authors would like to thank technicians Susana Couto and Paula Menezes from the Materials Department and Ana Maria Francisco from the Buildings Department of LNEC, for their support in the execution of the experimental work. 8 4 141-151pp Volume 7, RILEM Bookseries DM/NMM

Published
2012

20. Characterization of Decorative Portuguese Gypsum Plasters from the XIX-XX Centuries: The Case of the Bolsa Palace in Oporto

Author

Freire, T., Santos Silva, A., Veiga, M. R., Brito, J., and J. Válek, C. Groot, J. J. Hughes

Subjects
Characterization, Gypsum plasters, Conservation, Compatibility
Abstract

In order to achieve the issues of compatibility for the design of new repair products a complete characterization of the original materials is of extreme importance. Gypsum plaster materials are not an exception to that rule. The characterization work of the Portuguese historical gypsum plasters has never been done as well as the study of efficient solutions for their preservation. In this paper the results of the characterization of gypsum plaster samples from the second half of the XIX century belonging to the Arabian Room of the Bolsa Palace, located in Oporto, North of Portugal, are presented and discussed. XRD, TGA-DTA, optical microscopy and FESEM-EDS observations were used for the chemical and microstructural characterization. Some physical properties, such as dynamic modulus of elasticity and capillary absorption were also determined. A relationship between the results obtained, namely composition and characteristics observed in the samples, and the technology associated to their use and application on site is established. Teresa Freire’s PhD research study is being supported by the scholarship SFRH/BD/40128/2007 from Fundação para a Ciência e Tecnologia (FCT). The authors would also like to thank technicians Susana Couto and Paula Menezes from Materials Department and Ana Maria Francisco from the Buildings Department of LNEC, for their support on the execution of the experimental work. 6 5 165-174pp DM/NMM 2010 22 a 24 de Setembro

Published
2010
Full Text
View/download PDF

21. Caracterização de Revestimentos Interiores Antigos Portugueses

Author

Freire, T., Santos Silva, A., Veiga, M. R., and Brito, J.

Subjects
Caracterização, Preservação do património, Revestimentos interiores
Abstract

Submitted by António Santos Silva (ssilva@lnec.pt) on 2010-04-07T13:30:06Z No. of bitstreams: 1 Paper 11_2010 Teresa Freire.pdf: 329446 bytes, checksum: a2bce2c12411726f770960095c490c3b (MD5) Approved for entry into archive by mpnunes@lnec.pt(mpnunes@lnec.pt) on 2010-04-08T09:23:46Z (GMT) No. of bitstreams: 1 Paper 11_2010 Teresa Freire.pdf: 329446 bytes, checksum: a2bce2c12411726f770960095c490c3b (MD5) Made available in DSpace on 2010-04-08T09:23:47Z (GMT). No. of bitstreams: 1 Paper 11_2010 Teresa Freire.pdf: 329446 bytes, checksum: a2bce2c12411726f770960095c490c3b (MD5) Previous issue date: 2010-03-18 Made available in DSpace on 2010-04-27T07:25:00Z (GMT). No. of bitstreams: 2 license.txt: 1838 bytes, checksum: 3e6fbff9fdaf02ecc777380944675a19 (MD5) Paper 11_2010 Teresa Freire.pdf: 329446 bytes, checksum: a2bce2c12411726f770960095c490c3b (MD5) Previous issue date: 2010-03-18 Made available in DSpace on 2014-10-20T16:32:12Z (GMT). No. of bitstreams: 2 Paper 11_2010 Teresa Freire.pdf: 329446 bytes, checksum: a2bce2c12411726f770960095c490c3b (MD5) license.txt: 1838 bytes, checksum: 3e6fbff9fdaf02ecc777380944675a19 (MD5) Previous issue date: 2010-03-18 Made available in DSpace on 2017-04-12T09:17:59Z (GMT). No. of bitstreams: 2 license.txt: 1838 bytes, checksum: 3e6fbff9fdaf02ecc777380944675a19 (MD5) Paper 11_2010 Teresa Freire.pdf: 329446 bytes, checksum: a2bce2c12411726f770960095c490c3b (MD5) Previous issue date: 2010-03-18 O trabalho de investigação em curso é financiado pela bolsa de doutoramento SFRH/BD/40128/2007 da Fundação para a Ciência e Tecnologia (FCT), atribuída à primei-ra autora, Teresa Freire. Os autores gostariam também de agradecer a colaboração das técnicas Susana Couto e Paula Menezes, do Departamento de Materiais do LNEC, na execução do trabalho experi-mental. 15 4 14 DM/NMM 2010 18 e 19 de Março de 2010

Published
2010

22. Characterization of a 19TH century decorated gypsum plaster piece: the role of microscopy

Author

Freire, T., Santos Silva, A., Veiga, M. R., and Brito, J.

Subjects
Microscopy, Portuguese gypsum plasters, Characterization, Cultural heritage
Abstract

Submitted by António Santos Silva (ssilva@lnec.pt) on 2009-09-25T15:48:49Z No. of bitstreams: 1 T.Freire et al. EA.pdf: 21202 bytes, checksum: f57895f5e07dbfe0d7e854c030c169e9 (MD5) Approved for entry into archive by mpnunes@lnec.pt(mpnunes@lnec.pt) on 2009-10-08T11:29:11Z (GMT) No. of bitstreams: 1 T.Freire et al. EA.pdf: 21202 bytes, checksum: f57895f5e07dbfe0d7e854c030c169e9 (MD5) Made available in DSpace on 2009-10-08T11:29:12Z (GMT). No. of bitstreams: 1 T.Freire et al. EA.pdf: 21202 bytes, checksum: f57895f5e07dbfe0d7e854c030c169e9 (MD5) Previous issue date: 2009-09 Made available in DSpace on 2010-04-27T07:26:37Z (GMT). No. of bitstreams: 2 license.txt: 1838 bytes, checksum: d310ff4250b5f4b9e7be1027f88a00bd (MD5) T.Freire et al. EA.pdf: 21202 bytes, checksum: f57895f5e07dbfe0d7e854c030c169e9 (MD5) Previous issue date: 2009-09 Made available in DSpace on 2014-10-20T16:32:22Z (GMT). No. of bitstreams: 2 T.Freire et al. EA.pdf: 21202 bytes, checksum: f57895f5e07dbfe0d7e854c030c169e9 (MD5) license.txt: 1838 bytes, checksum: d310ff4250b5f4b9e7be1027f88a00bd (MD5) Previous issue date: 2009-09 Made available in DSpace on 2017-04-13T10:14:54Z (GMT). No. of bitstreams: 2 license.txt: 1838 bytes, checksum: d310ff4250b5f4b9e7be1027f88a00bd (MD5) T.Freire et al. EA.pdf: 21202 bytes, checksum: f57895f5e07dbfe0d7e854c030c169e9 (MD5) Previous issue date: 2009-09 Teresa Freire’s PhD research study is being supported by the scholarship SFRH/BD/40128/2007 from Fundação para a Ciência e Tecnologia (FCT). The authors would like to thank the technicians Susana Couto and Paula Menezes from Materials Department and Dora Santos, from the Buildings Department of LNEC, for their support on the execution of the experimental work. They would also like to thank to Isabel Pombo Cardoso, PhD student from the University College of London, for the interesting and helpful discussions and to Professor José Mirão from University of Évora for the precious help on the observation of the thin sections. 15 4 13 DM/NMM 2009 15 a 19 de Setembro

Published
2009

24. Happiness through leisure

Author

Freire, T., Nawijn, J (Jeroen), Veenhoven, R, Freire, T., Nawijn, J (Jeroen), and Veenhoven, R

Published
2013

27. Criterios para el diagnóstico de la práctica educativa a distancia en modalidad BLearning

Author

Freire Tigreros, María Eufemia, Gómez Zermeño, Marcela Georgina, and Garcia Vázquez, Nancy Yaneth

Subjects
Ciencias Informáticas, Education, Special aspects of education, LC8-6691
Abstract

La propuesta de investigación se contextualiza en una institución de Educación Superior ubicada en la ciudad de Cali, en Colombia, con el propósito de evaluar la práctica educativa en modalidad B-learning de un programa de educación superior, en base a los principios de flexibilidad, interactividad, cooperación y autogestión con el propósito de plantear recomendaciones y un plan de mejora que ayude a que los procesos de enseñanzaaprendizaje en la educación a distancia. Se utilizó una metodología cuantitativa para la recopilación y análisis de datos obtenidos de los cuestionarios a alumnos. Los resultados de investigación pretenden generar un diagnóstico que contribuya a la mejora del modelo Blearning en los diferentes niveles educativos.

Published
2016

29. Ocorrência de Myrothecium roridum Tode ex Fr. sobre juta (Corchorus capsularis L.) na Amazônia

Author

FREIRE, F. das C. O., FREIRE, T. de A., FRANCISCO DAS CHAGAS OLIVEIRA FREIRE, CPATU, and TEREZINHA DE ALMEIDA FREIRE, ESTAGIÁRIA CPATU.

Subjects
Fungo, Juta, Patógeno, Doença, Parasita
Abstract

Made available in DSpace on 2021-04-14T19:34:16Z (GMT). No. of bitstreams: 1 Ocorrencia-de-myrothecium.pdf: 2575697 bytes, checksum: b3f774edeaf71a1ee67c23eb3e618445 (MD5) Previous issue date: 1980

Published
1980

30. Possible molecular mechanisms soy-mediated in preventing and treating nonalcoholic fatty liver disease.

Author

Oliveira, L. P. M., de Jesús, R. P., Freire, T. O., Oliveira, C. P., Lyra, A. Castro, and Lyra, L. G. C.

Subjects
*FATTY liver, *SOYBEAN -- Nutrition, *DIETARY proteins, *DIETARY supplements, *FATTY degeneration, *LIVER disease prevention, *SOYBEAN, *THERAPEUTICS, *NUTRITION
Abstract

The aim of this review is to describe the molecular mechanisms of nonalcoholic fatty liver disease (NAFLD) and to present evidence regarding the mechanisms of soy-mediated therapeutic activity in preventing and treating NAFLD. NAFLD is induced by multiple metabolic path- ways, including an increase in the release of fatty acids from the adipose tissue (lipolysis), insulin resistance (IR), and an increase in "de novo" fatty acid synthesis. Furthermore, NAFLD is correlated with a decrease in liver β- oxidation, an increase in oxygen free radical production, and an increase in pro-inflammatory cytokine production, which leads to an increase in liver fat and, subsequently, to tissue damage. The bioactive compounds in soy can prevent and treat NAFLD by modulating lipid metabolism and regulating the expression of related transcription factors. Soy intake decreases the expression of sterol regulatory-element binding protein-1c (SREBP-1) and increases the expression of SREBP-2, which are transcription factors associated with the regulation of hepatic lipogenesis and reduction of cholesterol synthesis and absorption in the liver, respectively. Besides, interactions between soy components, such as standard amino acids, polyunsaturated fat, and the isoflavonoid-enriched fraction, are believed to improve fatty acid oxidation in the liver parenchyma by increasing the expression of peroxisome proliferator-activated receptor α (PPARa)-regulated genes, thus decreasing lipid accumulation in the liver. Therefore, including soy-derived foods in the diet as a therapeutic tool for patients with NAFLD might improve their clinical evolution. [ABSTRACT FROM AUTHOR]

Published
2012
Full Text
View/download PDF

32. Advances in surface design and biomedical applications of magnetic nanoparticles.

Author

Araújo EV, Carneiro SV, Neto DMA, Freire TM, Costa VM, Freire RM, Fechine LMUD, Clemente CS, Denardin JC, Dos Santos JCS, Santos-Oliveira R, Rocha JS, and Fechine PBA

Subjects
Animals, Humans, Drug Delivery Systems, Magnetic Resonance Imaging, Magnetite Nanoparticles chemistry, Surface Properties, Metal Nanoparticles chemistry
Abstract

Despite significant efforts by scientists in the development of advanced nanotechnology materials for smart diagnosis devices and drug delivery systems, the success of clinical trials remains largely elusive. In order to address this biomedical challenge, magnetic nanoparticles (MNPs) have gained attention as a promising candidate due to their theranostic properties, which allow the simultaneous treatment and diagnosis of a disease. Moreover, MNPs have advantageous characteristics such as a larger surface area, high surface-to-volume ratio, enhanced mobility, mass transference and, more notably, easy manipulation under external magnetic fields. Besides, certain magnetic particle types based on the magnetite (Fe 3 O 4 ) phase have already been FDA-approved, demonstrating biocompatible and low toxicity. Typically, surface modification and/or functional group conjugation are required to prevent oxidation and particle aggregation. A wide range of inorganic and organic molecules have been utilized to coat the surface of MNPs, including surfactants, antibodies, synthetic and natural polymers, silica, metals, and various other substances. Furthermore, various strategies have been developed for the synthesis and surface functionalization of MNPs to enhance their colloidal stability, biocompatibility, good response to an external magnetic field, etc. Both uncoated MNPs and those coated with inorganic and organic compounds exhibit versatility, making them suitable for a range of applications such as drug delivery systems (DDS), magnetic hyperthermia, fluorescent biological labels, biodetection and magnetic resonance imaging (MRI). Thus, this review provides an update of recently published MNPs works, providing a current discussion regarding their strategies of synthesis and surface modifications, biomedical applications, and perspectives., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Published
2024
Full Text
View/download PDF

33. Paternal dietary macronutrient balance and energy intake drive metabolic and behavioral differences among offspring.

Author

Crean AJ, Senior AM, Freire T, Clark TD, Mackay F, Austin G, Pulpitel TJ, Nobrega MA, Barrès R, and Simpson SJ

Subjects
Humans, Male, Female, Mice, Animals, Energy Intake, Nutrients, Carbohydrates, Dietary Fats, Diet, High-Fat, Diet, Fathers
Abstract

Paternal diet can influence the phenotype of the next generation, yet, the dietary components inducing specific responses in the offspring are not identified. Here, we use the Nutritional Geometry Framework to determine the effects of pre-conception paternal dietary macronutrient balance on offspring metabolic and behavioral traits in mice. Ten isocaloric diets varying in the relative proportion of protein, fats, and carbohydrates are fed to male mice prior to mating. Dams and offspring are fed standard chow and never exposed to treatment diets. Body fat in female offspring is positively associated with the paternal consumption of fat, while in male offspring, an anxiety-like phenotype is associated to paternal diets low in protein and high in carbohydrates. Our study uncovers that the nature and the magnitude of paternal effects are driven by interactions between macronutrient balance and energy intake and are not solely the result of over- or undernutrition., (© 2024. The Author(s).)

Published
2024
Full Text
View/download PDF

34. Truncated O-glycosylation in metastatic triple-negative breast cancer reveals a gene expression signature associated with extracellular matrix and proteolysis.

Author

Festari MF, Jara E, Costa M, Iriarte A, and Freire T

Subjects
Mice, Humans, Female, Animals, Transcriptome, Glycosylation, Proteolysis, Extracellular Matrix metabolism, Cell Line, Tumor, Triple Negative Breast Neoplasms pathology
Abstract

Breast cancer (BC) is the leading cause of death by cancer in women worldwide. Triple-negative (TN) BC constitutes aggressive and highly metastatic tumors associated with shorter overall survival of patients compared to other BC subtypes. The Tn antigen, a glycoconjugated structure resulting from an incomplete O-glycosylation process, is highly expressed in different adenocarcinomas, including BC. It also favors cancer growth, immunoregulation, and metastasis in TNBC. This work describes the differentially expressed genes (DEGs) associated with BC aggressiveness and metastasis in an incomplete O-glycosylated TNBC cell model. We studied the transcriptome of a TNBC model constituted by the metastatic murine 4T1 cell line that overexpresses the Tn antigen due to a mutation in one of the steps of the O-glycosylation pathway. We analyzed and compared the results with the parental wild-type cell line and with a Tn-negative cell clone that was poorly metastatic and less aggressive than the 4T1 parental cell line. To gain insight into the generated expression data, we performed a gene set analysis. Biological processes associated with cancer development and metastasis, immune evasion, and leukocyte recruitment were highly enriched among functional terms of DEGs. Furthermore, different highly O-glycosylated protein-coding genes, such as mmp9, ecm1 and ankyrin-2, were upregulated in 4T1/Tn + tumor cells. The altered biological processes and DEGs that promote tumor growth, invasion and immunomodulation might explain the aggressive properties of 4T1/Tn + tumor cells. These results support the hypothesis that incomplete O-glycosylation that leads to the expression of the Tn antigen, which might regulate activity or interaction of different molecules, promotes cancer development and immunoregulation., (© 2024. The Author(s).)

Published
2024
Full Text
View/download PDF

35. Toward reconciling the roles of FGF21 in protein appetite, sweet preference, and energy expenditure.

Author

Solon-Biet SM, Clark X, Bell-Anderson K, Rusu PM, Perks R, Freire T, Pulpitel T, Senior AM, Hoy AJ, Aung O, Le Couteur DG, Raubenheimer D, Rose AJ, Conigrave AD, and Simpson SJ

Subjects
Mice, Animals, Feeding Behavior, Energy Metabolism, Liver metabolism, Appetite, Fibroblast Growth Factors metabolism
Abstract

Fibroblast growth factor 21 (FGF21), an endocrine signal robustly increased by protein restriction independently of an animal's energy status, exerts profound effects on feeding behavior and metabolism. Here, we demonstrate that considering the nutritional contexts within which FGF21 is elevated can help reconcile current controversies over its roles in mediating macronutrient preference, food intake, and energy expenditure. We show that FGF21 is primarily a driver of increased protein intake in mice and that the effect of FGF21 on sweet preference depends on the carbohydrate balance of the animal. Under no-choice feeding, FGF21 infusion either increased or decreased energy expenditure depending on whether the animal was fed a high- or low-energy diet, respectively. We show that while the role of FGF21 in mediating feeding behavior is complex, its role in promoting protein appetite is robust and that the effects on sweet preference and energy expenditure are macronutrient-state-dependent effects of FGF21., Competing Interests: Declaration of interests The authors declare no competing interests., (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.)

Published
2023
Full Text
View/download PDF

36. Modeling spatial evolution of multi-drug resistance under drug environmental gradients.

Author

Freire T, Hu Z, Wood KB, and Gjini E

Abstract

Multi-drug combinations to treat bacterial populations are at the forefront of approaches for infection control and prevention of antibiotic resistance. Although the evolution of antibiotic resistance has been theoretically studied with mathematical population dynamics models, extensions to spatial dynamics remain rare in the literature, including in particular spatial evolution of multi-drug resistance. In this study, we propose a reaction-diffusion system that describes the multi-drug evolution of bacteria, based on a rescaling approach (Gjini and Wood, 2021). We show how the resistance to drugs in space, and the consequent adaptation of growth rate is governed by a Price equation with diffusion. The covariance terms in this equation integrate features of drug interactions and collateral resistances or sensitivities to the drugs. We study spatial versions of the model where the distribution of drugs is homogeneous across space, and where the drugs vary environmentally in a piecewise-constant, linear and nonlinear manner. Applying concepts from perturbation theory and reaction-diffusion equations, we propose an analytical characterization of average mutant fitness in the spatial system based on the principal eigenvalue of our linear problem. This enables an accurate translation from drug spatial gradients and mutant antibiotic susceptibility traits, to the relative advantage of each mutant across the environment. Such a mathematical understanding allows to predict the precise outcomes of selection over space, ultimately from the fundamental balance between growth and movement traits, and their diversity in a population.

Published
2023
Full Text
View/download PDF

37. How Is Leisure Related to Wellbeing and to Substance Use? The Probable Key Role of Autonomy and Supervision.

Author

Tomé G, Guedes FB, Cerqueira A, Noronha C, de Freitas JC, Freire T, and de Matos MG

Abstract

The present research is based on a large and representative national survey and intends to analyse the correlation of several leisure activities with risk, and with health and well-being outcomes. This work is part of the Health Behaviour in School-aged Children (HBSC) study, a collaborative WHO international study that aims to explore the school-aged children behaviour regarding health and risk behaviours in their life contexts. Participants were 8215 Portuguese adolescents, randomly chosen from those attending the 6th, 8th, 10th and 12th grades in 2018. The sample included 52.7% of girls and the mean age was 14.36 years old. Descriptive and comparative analyses were performed (ANOVAS and Chi-Square). The results of the present study suggested that several leisure activities, namely sports and social engagement activities (politic involvement and participation, religious activities, scouting and volunteer work), are associated with the adolescents' well-being and life satisfaction. However, these types of activities can also be associated with an increase in substance use. However, some activities are also associated with risky behaviour. Identifying activities that promote well-being in young people can be important for professionals, families and public policies.

Published
2023
Full Text
View/download PDF

38. Difference in Structure and Electronic Properties of Oxygen Vacancies in α -Quartz and α -Cristobalite Phases of SiO 2 .

Author

Milton KL, Durrant TR, Cobos Freire T, and Shluger AL

Abstract

α-cristobalite (α-C) is a polymorph of silica, mainly found in space exploration and geochemistry research. Due to similar densities, α-C is often used as a proxy for amorphous SiO2, particularly in computer simulations of SiO2 surfaces and interfaces. However, little is known about the properties of α-C and its basic oxygen defects. Using density functional theory (DFT) simulations we provide a comprehensive report on the properties of perfect structure and oxygen vacancies in α-C. The calculated properties of α-C are compared with those of the better-characterized α-quartz (α-Q). Our results demonstrated that the positively charged O vacancy in α-C is most stable in the dimer configuration, in contrast to α-Q, which favors the puckered configuration. A back-projected configuration was also predicted in both polymorphs. We calculated the optical transition energies and isotropic hyperfine constants for O vacancies in both α-Q and α-C, and compared our findings with the results of previous studies and experiments. This work, thus, offers one of the first in-depth investigations of the properties of oxygen vacancies in α-C.

Published
2023
Full Text
View/download PDF

39. Trypanosoma cruzi -Derived Molecules Induce Anti-Tumour Protection by Favouring Both Innate and Adaptive Immune Responses.

Author

Freire T, Landeira M, Giacomini C, Festari MF, Pittini Á, Cardozo V, Brosque A, Monin L, da Costa V, Faral-Tello P, Robello C, and Osinaga E

Subjects
Humans, Mice, Animals, Interferon-gamma, Killer Cells, Natural, Adaptive Immunity, Trypanosoma cruzi, Chagas Disease, Neoplasms
Abstract

Lung cancer remains the leading cause of cancer mortality worldwide. Thus, the development of strategies against this type of cancer is of high value. Parasite infections can correlate with lower cancer incidence in humans and their use as vaccines has been recently explored in preclinical models. In this study, we investigated whether immunisations with a Trypanosoma cruzi lysate from epimastigotes protect from lung tumour growth in mice. We also explore the role of parasite glycans in the induction of the protective immune response. A pre-clinical murine cancer model using the lung tumour cell line LL/2 was used to evaluate the anti-tumour potential, both in preventive and therapeutic settings, of a T. cruzi epimastigote-derived protein lysate. Immunisation with the parasite lysate prevents tumour growth and induces both humoral and cellular anti-tumour immune responses to LL-2 cancer cells. The induced immunity and tumour protection were associated with the activation of natural killer (NK) cells, the production of interferon-γ (IFN-γ) and tumour cell cytotoxicity. We also show that mannose residues in the T. cruzi lysate induce Toll-like receptor (TLR) signalling. The evaluated T. cruzi lysate possesses anti-tumour properties likely by activating innate and adaptive immunity in a process where carbohydrates seem to be essential.

Published
2022
Full Text
View/download PDF

40. Macrophage Gal/GalNAc lectin 2 (MGL2) + peritoneal antigen presenting cells during Fasciola hepatica infection are essential for regulatory T cell induction.

Author

Costa M, da Costa V, Lores P, Landeira M, Rodríguez-Zraquia SA, Festari MF, and Freire T

Subjects
Humans, Mice, Animals, Heparin-binding EGF-like Growth Factor, T-Lymphocytes, Regulatory, Lectins, C-Type genetics, Antigen-Presenting Cells, Glycoconjugates, Macrophages, Forkhead Transcription Factors, Fasciola hepatica, Fascioliasis parasitology
Abstract

Fasciola hepatica, one of the agents that causes fasciolosis, modulates the host immune system to allow parasite survival in the host. F. hepatica expresses carbohydrate-containing glycoconjugates that are decoded by C-type lectin receptors, such as Dectin-1, mannose receptor, DC-SIGN and MGL, that are mainly present on myeloid antigen presenting cells (APCs) and can mediate immunoregulatory properties on T cells. In particular, Macrophage Gal/GalNAc lectin 2 (MGL2) expands modified Th2 immune responses, while suppressing Th1 polarization, upon recognition of GalNAc-glycosylated parasite components. In this study, by using MGL2-DTR transgenic mice that encode human diphtheria toxin receptor in MGL2 + cells, we demonstrate the role of peritoneal APCs during F. hepatica infection in favoring parasite survival. This process might be mediated by the induction of splenic Tregs in vivo, since the depletion of MGL2 + cells conferred mice with partial resistance to the infection and abrogated the increase of CD4 + /CD25 +  FoxP3 + Tregs induced by the parasite. Therefore, MGL2 + cells are critical determinants of F. hepatica infection and could constitute immune checkpoints to control parasite infection., (© 2022. The Author(s).)

Published
2022
Full Text
View/download PDF

41. Lung Tumor Cells with Different Tn Antigen Expression Present Distinctive Immunomodulatory Properties.

Author

Costa VD, Mariño KV, Rodríguez-Zraquia SA, Festari MF, Lores P, Costa M, Landeira M, Rabinovich GA, van Vliet SJ, and Freire T

Subjects
Animals, Antigens, Tumor-Associated, Carbohydrate chemistry, Galactosamine, Lectins, Lung metabolism, Mice, Serine, Threonine, Galactose, Lung Neoplasms genetics
Abstract

Lung cancer is the first leading cause of cancer-related deaths in the world. Aberrant glycosylation in lung tumors leads to the expression of tumor-associated carbohydrate structures, such as the Tn antigen, consisting of N -acetyl-galactosamine (GalNAc) linked to a serine or threonine residue in proteins (α-GalNAc-O-Ser/Thr). The Tn antigen can be recognized by the Macrophage Galactose/GalNAc lectin (MGL), which mediates various immune regulatory and tolerogenic functions, mainly by reprogramming the maturation of function of dendritic cells (DCs). In this work, we generated two different Tn-expressing variants from the Lewis-type lung murine cancer cell line LL/2, which showed different alterations in the O -glycosylation pathways that influenced the interaction with mouse MGL2 and the immunomodulatory properties of DCs. Thus, the identification of the biological programs triggered by Tn + cancer cells might contribute to an improved understanding of the molecular mechanisms elicited by MGL-dependent immune regulatory circuits.

Published
2022
Full Text
View/download PDF

42. The tumor-associated Tn antigen fosters lung metastasis and recruitment of regulatory T cells in triple negative breast cancer.

Author

Festari MF, da Costa V, Rodríguez-Zraquia SA, Costa M, Landeira M, Lores P, Solari-Saquieres P, Kramer MG, and Freire T

Subjects
Animals, Antigens, Tumor-Associated, Carbohydrate, Cell Line, Tumor, Humans, Mice, Prognosis, T-Lymphocytes, Regulatory, Lung Neoplasms, Triple Negative Breast Neoplasms metabolism, Triple Negative Breast Neoplasms pathology
Abstract

Cancer is a leading cause of death worldwide, accounting for nearly 10 million deaths. Among breast cancers (BC) subtypes, triple-negative (TN) BC is characterized by metastatic progression and poor patient prognosis. Although, TNBC is initially sensitive to chemotherapy, many TNBC patients rapidly develop resistance, at which point metastatic disease is highly lethal. Cancer cells present phenotypic changes or molecular signatures that distinguish them from healthy cells. The Tn antigen (GalNAc-O-Thr/Ser), which constitutes a powerful tool as tumor marker, was recently reported to contribute to tumor growth. However, its role in BC-derived metastasis has not yet been addressed. In this work, we generated a pre-clinical orthotopic Tn+ model of metastatic TNBC, which mimics the patient surgical treatment and is useful to study the role of Tn in metastasis and immunoregulation. We obtained two different cell clones, which differed in their Tn antigen expression: a high Tn-expressing and a non-expressing clone. Interestingly, the Tn-positive cell line generated significantly larger tumors and higher degree of lung metastases associated with a lower survival rate than the Tn-negative and parental cell line. Furthermore, we also found that both tumors and draining-lymph nodes from Tn+-tumor-bearing mice presented a higher frequency of CD4+ FoxP3+ T cells, while their splenocytes expressed higher levels of IL-10. In conclusion, this work suggests that the Tn antigen participates in breast tumor growth and spreading, favoring metastases to the lungs that are associated with an immunoregulatory state, suggesting that Tn-based immunotherapy could be a strategy of choice to treat these tumors., (© The Author(s) 2022. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Published
2022
Full Text
View/download PDF

43. Advances in the Immunomodulatory Properties of Glycoantigens in Cancer.

Author

da Costa V and Freire T

Abstract

Aberrant glycosylation in tumour progression is currently a topic of main interest. Tumour-associated carbohydrate antigens (TACAs) are expressed in a wide variety of epithelial cancers, being both a diagnostic tool and a potential treatment target, as they have impact on patient outcome and disease progression. Glycans affect both tumour-cell biology properties as well as the antitumor immune response. It has been ascertained that TACAs affect cell migration, invasion and metastatic properties both when expressed by cancer cells or by their extracellular vesicles. On the other hand, tumour-associated glycans recognized by C-type lectin receptors in immune cells possess immunomodulatory properties which enable tumour growth and immune response evasion. Yet, much remains unknown, concerning mechanisms involved in deregulation of glycan synthesis and how this affects cell biology on a major level. This review summarises the main findings to date concerning how aberrant glycans influence tumour growth and immunity, their application in cancer treatment and spotlights of unanswered challenges remaining to be solved.

Published
2022
Full Text
View/download PDF

44. A Scoping Review of Flow Research.

Author

Peifer C, Wolters G, Harmat L, Heutte J, Tan J, Freire T, Tavares D, Fonte C, Andersen FO, van den Hout J, Šimleša M, Pola L, Ceja L, and Triberti S

Abstract

Flow is a gratifying state of deep involvement and absorption that individuals report when facing a challenging activity and they perceive adequate abilities to cope with it (EFRN, 2014). The flow concept was introduced by Csikszentmihalyi in 1975, and interest in flow research is growing. However, to our best knowledge, no scoping review exists that takes a systematic look at studies on flow which were published between the years 2000 and 2016. Overall, 252 studies have been included in this review. Our review (1) provides a framework to cluster flow research, (2) gives a systematic overview about existing studies and their findings, and (3) provides an overview about implications for future research. The provided framework consists of three levels of flow research. In the first "Individual" level are the categories for personality, motivation, physiology, emotion, cognition, and behavior. The second "Contextual" level contains the categories for contextual and interindividual factors and the third "Cultural" level contains cultural factors that relate to flow. Using our framework, we systematically present the findings for each category. While flow research has made progress in understanding flow, in the future, more experimental and longitudinal studies are needed to gain deeper insights into the causal structure of flow and its antecedents and consequences., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Peifer, Wolters, Harmat, Heutte, Tan, Freire, Tavares, Fonte, Andersen, van den Hout, Šimleša, Pola, Ceja and Triberti.)

Published
2022
Full Text
View/download PDF

45. Adolescer in Time of COVID-19's Pandemic: Rationale and Construction Process of a Digital Intervention to Promote Adolescents' Positive Development.

Author

Freire T, Santana G, Vieira A, and Barbosa B

Subjects
Adaptation, Psychological, Adolescent, Adolescent Development, Child, Humans, SARS-CoV-2, COVID-19 epidemiology, Pandemics
Abstract

The coronavirus pandemic has severely impacted children's and adolescents' lives due to policies and regulations implemented to slow the virus from spreading, which led to a loss of routine, structure, academic support, and social contacts. Literature also reports a lack of outdoor activity, inappropriate diet, and disruption of sleeping habits as affecting children's and adolescents' lifestyles and well-being. Remarkably, these consequent psychological, behavioral, and emotional changes can compromise their self-esteem, sense of self-efficacy, and self-concept, affecting their immune systems. These maladaptive coping strategies and associated effects may emerge as a failure to access some of the sources of support that might help them cope. Facing this crisis, we aimed at promoting well-being, growth, and the positive development of Portuguese adolescents through an intervention focused on positive coping strategies. We developed " Adolescer in time of COVID-19-A good practices Guide for adolescents in social distancing" as a digital document to be quickly disseminated online, answering the emergent needs of Portuguese youth between 13 and 18 years old during the COVID-19 pandemic. In this article, we present the rationale and process of construction of this intervention while living within a quarantine period, considering the restrictive measures adopted at the time.

Published
2022
Full Text
View/download PDF

46. Heme-Oxygenase-1 Attenuates Oxidative Functions of Antigen Presenting Cells and Promotes Regulatory T Cell Differentiation during Fasciola hepatica Infection.

Author

Costa M, da Costa V, Frigerio S, Festari MF, Landeira M, Rodríguez-Zraquia SA, Lores P, Carasi P, and Freire T

Abstract

Fasciola hepatica is a fluke that infects livestock and humans causing fasciolosis, a zoonotic disease of increasing importance due to its worldwide distribution and high economic losses. The parasite regulates the host immune system by inducing a strong Th2 and regulatory T (Treg) cell immune response through mechanisms that might involve the expression or activity of heme-oxygenase-1 (HO-1), the rate-limiting enzyme in the catabolism of free heme that also has immunoregulatory and antioxidant properties. In this paper, we show that F. hepatica -infected mice upregulate HO-1 on peritoneal antigen-presenting cells (APC), which produce decreased levels of both reactive oxygen and nitrogen species (ROS/RNS). The presence of these cells was associated with increased levels of regulatory T cells (Tregs). Blocking the IL-10 receptor (IL-10R) during parasite infection demonstrated that the presence of splenic Tregs and peritoneal APC expressing HO-1 were both dependent on IL-10 activity. Furthermore, IL-10R neutralization as well as pharmacological treatment with the HO-1 inhibitor SnPP protected mice from parasite infection and allowed peritoneal APC to produce significantly higher ROS/RNS levels than those detected in cells from infected control mice. Finally, parasite infection carried out in gp91 phox knockout mice with inactive NADPH oxidase was associated with decreased levels of peritoneal HO-1 + cells and splenic Tregs, and partially protected mice from the hepatic damage induced by the parasite, revealing the complexity of the molecular mechanisms involving ROS production that participate in the complex pathology induced by this helminth. Altogether, these results contribute to the elucidation of the immunoregulatory and antioxidant role of HO-1 induced by F. hepatica in the host, providing alternative checkpoints that might control fasciolosis.

Published
2021
Full Text
View/download PDF

47. Human hydatid cyst fluid-induced therapeutic anti-cancer immune responses via NK1.1 + cell activation in mice.

Author

Berriel E, Freire T, Chiale C, Rodríguez E, Morón G, Fernández-Graña G, Crispo M, Berois N, and Osinaga E

Subjects
Animals, Cell Line, Tumor, Colonic Neoplasms immunology, Humans, Immunity immunology, Male, Mice, Mice, Inbred C57BL, Spleen immunology, Tumor Microenvironment immunology, Antigens, Ly immunology, Echinococcosis immunology, Echinococcus granulosus immunology, NK Cell Lectin-Like Receptor Subfamily B immunology
Abstract

Echinococcus granulosus is a cestode parasite which causes cystic echinococcosis disease. Previously we observed that vaccination with E. granulosus antigens from human hydatid cyst fluid (HCF) significantly inhibits colon cancer growth. In the present work, we evaluate the anti-tumor immune response induced by human HCF against LL/2 lung cancer in mice. HCF vaccination protected from tumor growth, both in prophylactic and therapeutic settings, and significantly increased mouse survival compared to control mice. Considering that tumor-associated carbohydrate antigens are expressed in E. granulosus, we oxidized terminal carbohydrates in HCF with sodium periodate. This treatment abrogates the anti-tumor activity induced by HCF vaccination. We found that HCF vaccination-induced IgG antibodies that recognize LL/2 tumor cells by flow cytometry. An antigen-specific immune response is induced with HCF vaccination in the tumor-draining lymph nodes and spleen characterized by the production of IL-5 and, in less extent, IFNɣ. In the tumor microenvironment, we found that NK1.1 positive cells from HCF-treated mice showed higher expression of CD69 than control mice ones, indicating a higher level of activation. When we depleted these cells by administrating the NK-specific antibody NK1.1, a significantly decreased survival was observed in HCF-induced mice, suggesting that NK1.1 + cells mediate the anti-tumor protection induced by HCF. These results suggest that HCF can evoke an integrated anti-tumor immune response involving both, the innate and adaptive components, and provide novel insights into the understanding of the intricate relationship between HCF vaccination and tumor growth., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Published
2021
Full Text
View/download PDF

48. Positive Youth Development in the Context of Climate Change: A Systematic Review.

Author

Pereira T and Freire T

Abstract

Climate change has been acknowledged as one of the most significant current threats for younger generations. However, few studies have focused on climate change impacts on youth and how they can be supported. The purpose of this systematic review is to emphasize that a developmental perspective is fundamental within the interdisciplinary studies concerning climate change. Specifically, we focus our research on how the Positive Youth Development framework may inform future approaches to promote adolescents' and young adults' well-being and engagement in the context of climate change. A systematic review was conducted following Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. The search comprised two databases, and a total of 13 articles were finally considered eligible for review. Data were analyzed using a narrative method. The results show that the Positive Youth Development theory is not yet directly embedded in existing studies concerning adolescents and young adults in the context of climate change, but some of its principles were identified. Examples are provided of how Positive Youth Development characteristics and constructs can enhance future research, practice, and policies. We highlight this framework as an innovative and promising approach in the context of climate change., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Pereira and Freire.)

Published
2021
Full Text
View/download PDF

49. The cytosolic tryparedoxin peroxidase from Trypanosoma cruzi induces a pro-inflammatory Th1 immune response in a peroxidatic cysteine-dependent manner.

Author

López L, Chiribao ML, Girard MC, Gómez KA, Carasi P, Fernandez M, Hernandez Y, Robello C, Freire T, and Piñeyro MD

Subjects
Adaptive Immunity, Adult, Aged, Animals, Case-Control Studies, Cell Proliferation, Cells, Cultured, Chagas Disease immunology, Chagas Disease parasitology, Female, Host-Parasite Interactions, Humans, Immunity, Innate, Male, Mice, Inbred BALB C, Middle Aged, Mutation, Peroxidases genetics, Peroxidases immunology, Protein Structure, Quaternary, Protozoan Proteins genetics, Protozoan Proteins immunology, Structure-Activity Relationship, Th1 Cells immunology, Th1 Cells parasitology, Trypanosoma cruzi genetics, Trypanosoma cruzi immunology, Mice, Chagas Disease metabolism, Cytokines metabolism, Inflammation Mediators metabolism, Lymphocyte Activation, Peroxidases metabolism, Protozoan Proteins metabolism, Th1 Cells metabolism, Trypanosoma cruzi enzymology
Abstract

Trypanosoma cruzi cytosolic tryparedoxin peroxidase (c-TXNPx) is a 2-Cys peroxiredoxin (Prx) with an important role in detoxifying host cell oxidative molecules during parasite infection. c-TXNPx is a virulence factor, as its overexpression enhances parasite infectivity and resistance to exogenous oxidation. As Prxs from other organisms possess immunomodulatory properties, we studied the effects of c-TXNPx in the immune response and analysed whether the presence of the peroxidatic cysteine is necessary to mediate these properties. To this end, we used a recombinant c-TXNPx and a mutant version (c-TXNPxC52S) lacking the peroxidatic cysteine. We first analysed the oligomerization profile, oxidation state and peroxidase activity of both proteins by gel filtration, Western blot and enzymatic assay, respectively. To investigate their immunological properties, we analysed the phenotype and functional activity of macrophage and dendritic cells and the T-cell response by flow cytometry after injection into mice. Our results show that c-TXNPx, but not c-TXNPxC52S, induces the recruitment of IL-12/23p40-producing innate antigen-presenting cells and promotes a strong specific Th1 immune response. Finally, we studied the cellular and humoral immune response developed in the context of parasite natural infection and found that only wild-type c-TXNPx induces proliferation and high levels of IFN-γ secretion in PBMC from chronic patients without demonstrable cardiac manifestations. In conclusion, we demonstrate that c-TXNPx possesses pro-inflammatory properties that depend on the presence of peroxidatic cysteine that is essential for peroxidase activity and quaternary structure of the protein and could contribute to rational design of immune-based strategies against Chagas disease., (© 2021 John Wiley & Sons Ltd.)

Published
2021
Full Text
View/download PDF

50. Macronutrient Determinants of Obesity, Insulin Resistance and Metabolic Health.

Author

Wali JA, Solon-Biet SM, Freire T, and Brandon AE

Abstract

Obesity caused by the overconsumption of calories has increased to epidemic proportions. Insulin resistance is often associated with an increased adiposity and is a precipitating factor in the development of cardiovascular disease, type 2 diabetes, and altered metabolic health. Of the various factors contributing to metabolic impairments, nutrition is the major modifiable factor that can be targeted to counter the rising prevalence of obesity and metabolic diseases. However, the macronutrient composition of a nutritionally balanced "healthy diet" are unclear, and so far, no tested dietary intervention has been successful in achieving long-term compliance and reductions in body weight and associated beneficial health outcomes. In the current review, we briefly describe the role of the three major macronutrients, carbohydrates, fats, and proteins, and their role in metabolic health, and provide mechanistic insights. We also discuss how an integrated multi-dimensional approach to nutritional science could help in reconciling apparently conflicting findings.

Published
2021
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results