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1. Dynamics of the African swine fever spread in Poland

Author

Bocian Łukasz, Frant Maciej, Ziętek-Barszcz Anna, Niemczuk Krzysztof, and Szczotka-Bochniarz Anna

Subjects
asf, spread, spatial analysis, geostatistical analysis, poland., Veterinary medicine, SF600-1100
Abstract

African swine fever (ASF) is a lethal haemorrhagic disease of Suidae, present in Poland since 2014. The natural reservoir of ASF in Europe is the wild boar (Sus scrofa); however, human activity facilitates long-distance introductions of the disease. In ASF control it is important to identify areas at increased risk of infection. Such identification and estimation of the disease’s progress and subsequent spread will help to identify the specific preventive action needs in given zones. Serving this purpose, this study is a spatial and statistical analysis of ASF spread through noted outbreak data.

Published
2022
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3. Label-Free Detection of African Swine Fever and Classical Swine Fever in the Point-of-Care Setting Using Photonic Integrated Circuits Integrated in a Microfluidic Device.

Author

Manessis, Georgios, Frant, Maciej, Podgórska, Katarzyna, Gal-Cisoń, Anna, Łyjak, Magdalena, Urbaniak, Kinga, Woźniakowski, Grzegorz, Denes, Lilla, Balka, Gyula, Nannucci, Lapo, Griol, Amadeu, Peransi, Sergio, Basdagianni, Zoitsa, Mourouzis, Christos, Giusti, Alessandro, and Bossis, Ioannis

Subjects
CLASSICAL swine fever, AFRICAN swine fever, MICROFLUIDIC devices, SWINE diseases, TECHNOLOGICAL innovations, TELECOMMUNICATION, VIRUS diseases
Abstract

Swine viral diseases have the capacity to cause significant losses and affect the sector's sustainability, a situation further exacerbated by the lack of antiviral drugs and the limited availability of effective vaccines. In this context, a novel point-of-care (POC) diagnostic device incorporating photonic integrated circuits (PICs), microfluidics and information, and communication technology into a single platform was developed for the field diagnosis of African swine fever (ASF) and classical swine fever (CSF). The device targets viral particles and has been validated using oral fluid and serum samples. Sensitivity, specificity, accuracy, precision, positive likelihood ratio (PLR), negative likelihood ratio (NLR), and diagnostic odds ratio (DOR) were calculated to assess the performance of the device, and PCR was the reference method employed. Its sensitivities were 80.97% and 79%, specificities were 88.46% and 79.07%, and DOR values were 32.25 and 14.21 for ASF and CSF, respectively. The proposed POC device and PIC sensors can be employed for the pen-side detection of ASF and CSF, thus introducing novel technological advancements in the field of animal diagnostics. The need for proper validation studies of POC devices is highlighted to optimize animal biosecurity. [ABSTRACT FROM AUTHOR]

Published
2024
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6. Attempts at the development of a recombinant African swine fever virus strain with abrogated EP402R, 9GL, and A238L gene structure using the CRISPR/Cas9 system

Author

Woźniakowski Grzegorz, Mazur-Panasiuk Natalia, Walczak Marek, Juszkiewicz Małgorzata, Frant Maciej, and Niemczuk Krzysztof

Subjects
african swine fever, crispr/cas9, ep402r, 9gl, a238l, Veterinary medicine, SF600-1100
Abstract

African swine fever (ASF) is a pressing economic problem in a number of Eastern European countries. It has also depleted the Chinese sow population by 50%. Managing the disease relies on culling infected pigs or hunting wild boars as sanitary zone creation. The constraints on the development of an efficient vaccine are mainly the virus’ mechanisms of host immune response evasion. The study aimed to adapt a field ASFV strain to established cell lines and to construct recombinant African swine fever virus (ASFV) strain.

Published
2020
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7. A multi gene-approach genotyping method identifies 24 genetic clusters within the genotype II-European African swine fever viruses circulating from 2007 to 2022

Author

European Commission, Gallardo, Carmina [0000-0003-3293-306X], Djadjovski, Igor [0000-0002-4820-3590], Krivko, Laura [0009-0000-3047-4061], Chondrokouki, Eleni [0000-0002-8476-1821], Frant, Maciej [0000-0002-7169-3735], Pileviciene, Simona [0000-0002-0262-3899], Gallardo, Carmina, Casado, Nadia, Soler, Alejandro, Djadjovski, Igor, Krivko, Laura, Madueño, Encarnación, Nieto, Raquel, Pérez, Covadonga, Simón, Alicia, Ivanova, Emiliya, Donescu, Daniel, Milicevik, Vesna, Chondrokouki, Eleni, Nurmoja, Imbi, Frant, Maciej, Feliziani, Francesco, Václavek, Petr, Pileviciene, Simona, Marisa, Arias, European Commission, Gallardo, Carmina [0000-0003-3293-306X], Djadjovski, Igor [0000-0002-4820-3590], Krivko, Laura [0009-0000-3047-4061], Chondrokouki, Eleni [0000-0002-8476-1821], Frant, Maciej [0000-0002-7169-3735], Pileviciene, Simona [0000-0002-0262-3899], Gallardo, Carmina, Casado, Nadia, Soler, Alejandro, Djadjovski, Igor, Krivko, Laura, Madueño, Encarnación, Nieto, Raquel, Pérez, Covadonga, Simón, Alicia, Ivanova, Emiliya, Donescu, Daniel, Milicevik, Vesna, Chondrokouki, Eleni, Nurmoja, Imbi, Frant, Maciej, Feliziani, Francesco, Václavek, Petr, Pileviciene, Simona, and Marisa, Arias

Abstract

African swine fever (ASF) is a contagious viral disease of pigs and wild boar that poses a major threat to the global swine industry. The genotype II African swine fever virus (ASFV) entered the European Union (EU) in 2014 and since then fourteen countries have been affected, Italy and North Macedonia being the last in 2022. While whole genome sequencing remains the gold standard for the identification of new genetic markers, sequencing of multiple loci with significant variations could be used as a rapid and cost-effective alternative to track outbreaks and study disease evolution in endemic areas.

Published
2023

8. Avian reticuloendotheliosis in chickens – an update on disease occurrence and clinical course

Author

Woźniakowski Grzegorz, Frant Maciej, and Mamczur Andrzej

Subjects
chickens, avian reticuloendotheliosis, avian reticuloendotheliosis virus, disease course, diagnosis, Veterinary medicine, SF600-1100
Abstract

Avian reticuloendotheliosis (RE) represents an important immunosuppressive disease of poultry. The occurrence of RE in both chickens and turkeys has an immunosuppressive effect and may lead to vaccination failures. Avian reticuloendotheliosis virus (REV) is widely distributed in different kinds of birds, causing subclinical infections. Another important issue adhering to this disease is contamination of vaccines against fowl pox (FP) and Marek’s disease (MD) with REV. The capability of REV to integrate into the genome of other larger DNA viruses complicates its diagnosis and prevention. There are no efficient vaccines against RE nor treatment, which also complicates how to limit its impact on poultry farming. This paper reviews the current state of knowledge of this important immunosuppressive agent of poultry emphasising the importance of this problem in terms of diagnosis of RE.

Published
2018
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9. African swine fever (ASF) and ticks. No risk of tick-mediated ASF spread in Poland and Baltic states

Author

Frant Maciej, Woźniakowski Grzegorz, and Pejsak Zygmunt

Subjects
african swine fever, asf virus, ticks, vector, Veterinary medicine, SF600-1100
Abstract

Infectious diseases of swine, particularly zoonoses, have had a significant influence on nutritional safety and availability of pig meat as high-energy protein product since the time that pigs were domesticated back in the 7th century BC. The main sources of swine infectious diseases include the so-called primary sources (direct infection, i.e. through contact with infected and sick animals) and secondary sources (contaminated meat products, slaughter products, and vectors, including ticks). At present, the most serious epidemiological and economic threat to swine breeding in Europe is African swine fever (ASF). This disease, originally coming from Africa, is incurable and causes death of infected pigs and wild boars during 7−10 days after infection. Among the various factors that influence the spread of ASF, important role is played by ticks from the genus Ornithodoros, mainly from the species Ornithodoros moubata. Research on the ASF indicates that other species of ticks can also transmit the virus to healthy pigs in laboratory conditions. Sylvatic and domestic cycles of ASF virus transmission, which have been described so far, require further studies and updating in order to point the potential new vectors in the Caucasus and Eastern Europe affected by the ASF. Effective methods of control and biosecurity may significantly slow down the spread of ASF, which undoubtedly is a major threat to world pig production and international swine trade.

Published
2017
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10. Epidemiological Characterization of African Swine Fever Dynamics in Ukraine, 2012–2023

Author

Bezymennyi, Maksym, primary, Tarasov, Oleksandr, additional, Kyivska, Ganna V., additional, Mezhenska, Nataliia A., additional, Mandyhra, Svitlana, additional, Kovalenko, Ganna, additional, Sushko, Mykola, additional, Hudz, Nataliia, additional, Skorokhod, Serhii V., additional, Datsenko, Roman, additional, Muzykina, Larysa, additional, Milton, Elaina, additional, Sapachova, Maryna A., additional, Nychyk, Serhii, additional, Halka, Ihor, additional, Frant, Maciej, additional, Huettmann, Falk, additional, Drown, Devin M., additional, Gerilovych, Anton, additional, Mezhenskyi, Andrii A., additional, Bortz, Eric, additional, and Lange, Christian E., additional

Published
2023
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12. A multi gene-approach genotyping method identifies 24 genetic clusters within the genotype II-European African swine fever viruses circulating from 2007 to 2022

Author

Gallardo, Carmina, Casado, Nadia, Soler, Alejandro, Djadjovski, Igor, Krivko, Laura, Madueno, Encarnacion, Nieto, Raquel, Perez, Covadonga, Simon, Alicia, Ivanova, Emiliya, Donescu, Daniel, Milićević, Vesna, Chondrokouki, Eleni, Nurmoja, Imbi, Frant, Maciej, Feliziani, Francesco, Vaclavek, Petr, Pileviciene, Simona, Marisa, Arias, Gallardo, Carmina, Casado, Nadia, Soler, Alejandro, Djadjovski, Igor, Krivko, Laura, Madueno, Encarnacion, Nieto, Raquel, Perez, Covadonga, Simon, Alicia, Ivanova, Emiliya, Donescu, Daniel, Milićević, Vesna, Chondrokouki, Eleni, Nurmoja, Imbi, Frant, Maciej, Feliziani, Francesco, Vaclavek, Petr, Pileviciene, Simona, and Marisa, Arias

Abstract

IntroductionAfrican swine fever (ASF) is a contagious viral disease of pigs and wild boar that poses a major threat to the global swine industry. The genotype II African swine fever virus (ASFV) entered the European Union (EU) in 2014 and since then fourteen countries have been affected, Italy and North Macedonia being the last in 2022. While whole genome sequencing remains the gold standard for the identification of new genetic markers, sequencing of multiple loci with significant variations could be used as a rapid and cost-effective alternative to track outbreaks and study disease evolution in endemic areas. Materials and methodsTo further our understanding of the epidemiology and spread of ASFV in Europe, 382 isolates collected during 2007 to 2022 were sequenced. The study was initially performed by sequencing the central variable region (CVR), the intergenic region (IGR) between the I73R and I329L genes and the O174L and K145R genes. For further discrimination, two new PCRs were designed to amplify the IGR between the 9R and 10R genes of the multigene family 505 (MGF505) and the IGR between the I329L and I215L genes. The sequences obtained were compared with genotype II isolates from Europe and Asia. ResultsThe combination of the results obtained by sequencing these variable regions allowed to differentiate the European II-ASFV genotypes into 24 different groups. In addition, the SNP identified in the IGR I329L-I215L region, not previously described, grouped the viruses from North Macedonia that caused the 2022 outbreaks with viruses from Romania, Bulgaria, Serbia and Greece, differentiating from other genotype II isolates present in Europe and Asia. Furthermore, tandem repeat sequence (TRS) within the 9R-10R genes of the multigene family 505 (MGF505) revealed eight different variants circulating. DiscussionThese findings describe a new multi-gene approach sequencing method that can be used in routine genotyping to determine the origin of new introductions in A

Published
2023

13. A multi gene-approach genotyping method identifies 24 genetic clusters within the genotype II-European African swine fever viruses circulating from 2007 to 2022

Author

Gallardo, Carmina, primary, Casado, Nadia, additional, Soler, Alejandro, additional, Djadjovski, Igor, additional, Krivko, Laura, additional, Madueño, Encarnación, additional, Nieto, Raquel, additional, Perez, Covadonga, additional, Simon, Alicia, additional, Ivanova, Emiliya, additional, Donescu, Daniel, additional, Milicevik, Vesna, additional, Chondrokouki, Eleni, additional, Nurmoja, Imbi, additional, Frant, Maciej, additional, Feliziani, Francesco, additional, Václavek, Petr, additional, Pileviciene, Simona, additional, and Marisa, Arias, additional

Published
2023
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15. Dynamics of African swine fever virus (ASFV) infection in domestic pigs infected with virulent, moderate virulent and attenuated genotype II ASFV European isolates

Author

Gallardo, Carmina [0000-0003-3293-306X], Cano-Gómez, Cristina [0000-0003-4979-983X], Cvetkova, Svetlana [0000-0003-2497-4785], Frant, Maciej [0000-0002-7169-3735], Woźniakowski, Grzegorz [0000-0002-1333-4275], Simón, Alicia [0000-0003-0907-4017], Arias, Marisa [0000-0001-6044-2968], Gallardo, Carmina, Soler, Alejandro, Nurmoja, Imbi, Cano-Gómez, Cristina, Cvetkova, Svetlana, Frant, Maciej, Woźniakowski, Grzegorz, Simón, Alicia, Pérez, Covadonga, Nieto, Raquel, Arias, Marisa, Gallardo, Carmina [0000-0003-3293-306X], Cano-Gómez, Cristina [0000-0003-4979-983X], Cvetkova, Svetlana [0000-0003-2497-4785], Frant, Maciej [0000-0002-7169-3735], Woźniakowski, Grzegorz [0000-0002-1333-4275], Simón, Alicia [0000-0003-0907-4017], Arias, Marisa [0000-0001-6044-2968], Gallardo, Carmina, Soler, Alejandro, Nurmoja, Imbi, Cano-Gómez, Cristina, Cvetkova, Svetlana, Frant, Maciej, Woźniakowski, Grzegorz, Simón, Alicia, Pérez, Covadonga, Nieto, Raquel, and Arias, Marisa

Abstract

This study aimed to compare the infection dynamics of three genotype II African swine fever viruses (ASFV) circulating in Europe. Eighteen domestic pigs divided into three groups were infected intramuscularly or by direct contact with two haemadsorbent ASFVs (HAD) from Poland (Pol16/DP/ OUT21) and Estonia (Est16/WB/Viru8), and with the Latvian non-HAD ASFV (Lv17/WB/Rie1). Parameters, such as symptoms, pathogenicity, and distribution of the virus in tissues, humoral immune response, and dissemination of the virus by blood, oropharyngeal and rectal routes, were investigated. The Polish ASFV caused a case of rapidly developing fatal acute disease, while the Estonian ASFV caused acute to sub-acute infections and two animals survived. In contrast, animals infected with the ASFV from Latvia developed a more subtle, mild, or even subclinical disease. Oral excretion was sporadic or even absent in the attenuated group, whereas in animals that developed an acute or sub-acute form of ASF, oral excretion began at the same time the ASFV was detected in the blood, or even 3 days earlier, and persisted up to 22 days. Regardless of virulence, blood was the main route of transmission of ASFV and infectious virus was isolated from persistently infected animals for at least 19 days in the attenuated group and up to 44 days in the group of moderate virulence. Rectal excretion was limited to the acute phase of infection. In terms of diagnostics, the ASFV genome was detected in contact pigs from oropharyngeal samples earlier than in blood, independently of virulence. Together with blood, both samples could allow to detect ASFV infection for a longer period. The results presented here provide quantitative data on the spread and excretion of ASFV strains of different virulence among domestic pigs that can help to better focus surveillance activities and, thus, increase the ability to detect ASF introductions earlier.

Published
2021

16. SPR-Based Detection of ASF Virus in Cells

Author

Capo, Alessandro, primary, Calabrese, Alessia, additional, Frant, Maciej, additional, Walczak, Marek, additional, Szczotka-Bochniarz, Anna, additional, Manessis, Georgios, additional, Bossis, Ioannis, additional, Staiano, Maria, additional, D’Auria, Sabato, additional, and Varriale, Antonio, additional

Published
2022
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17. Point-of-Care and Label-Free Detection of Porcine Reproductive and Respiratory Syndrome and Swine Influenza Viruses Using a Microfluidic Device with Photonic Integrated Circuits

Author

Manessis, Georgios, primary, Frant, Maciej, additional, Wozniakowski, Grzegorz, additional, Nannucci, Lapo, additional, Benedetti, Martina, additional, Denes, Lilla, additional, Gyula, Balka, additional, Gelasakis, Athanasios I., additional, Squires, Clare, additional, Recuero, Sara, additional, Sanchez, Carlos, additional, Griol, Amadeu, additional, Giusti, Alessandro, additional, and Bossis, Ioannis, additional

Published
2022
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20. Photonic Label-Free Biosensors for Fast and Multiplex Detection of Swine Viral Diseases

Author

Gómez-Gómez, Maribel, primary, Sánchez, Carles, additional, Peransi, Sergio, additional, Zurita, David, additional, Bellieres, Laurent, additional, Recuero, Sara, additional, Rodrigo, Manuel, additional, Simón, Santiago, additional, Camarca, Alessandra, additional, Capo, Alessandro, additional, Staiano, Maria, additional, Varriale, Antonio, additional, D’Auria, Sabato, additional, Manessis, Georgios, additional, Gelasakis, Athnasios I., additional, Bossis, Ioannis, additional, Balka, Gyula, additional, Dénes, Lilla, additional, Frant, Maciej, additional, Nannucci, Lapo, additional, Bonasso, Matteo, additional, Giusti, Alessandro, additional, and Griol, Amadeu, additional

Published
2022
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23. Complete Genome Sequence of Salmonella enterica subsp. enterica Serovar Kottbus Strain Kharkiv, Isolated from a Commercial Pork Production Facility in Ukraine

Author

Arefiev, Vasiliy, primary, Kovalenko, Ganna, additional, Frant, Maciej, additional, Chumachenko, Tetiana, additional, Polyvianna, Yuliia, additional, Pivnenko, Svitlana, additional, Bolotin, Vitaliy, additional, Mayboroda, Olga, additional, Solodiankin, Oleksii, additional, Tarasov, Oleksandr, additional, Bezyemenni, Maksym, additional, Lyon, Cora, additional, Redlinger, Matthew, additional, Sapachova, Maryna, additional, Mezhenskyi, Andrii A., additional, Ducluzeau, Anne-Lise, additional, Bortz, Eric, additional, Gerilovych, Anton, additional, and Drown, Devin M., additional

Published
2020
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25. Complete Genome Sequence of a Virulent African Swine Fever Virus from a Domestic Pig in Ukraine

Author

Kovalenko, Ganna, primary, Ducluzeau, Anne-Lise, additional, Ishchenko, Liudmyla, additional, Sushko, Mykola, additional, Sapachova, Maryna, additional, Rudova, Nataliia, additional, Solodiankin, Oleksii, additional, Gerilovych, Anton, additional, Dagdag, Ralf, additional, Redlinger, Matthew, additional, Bezymennyi, Maksym, additional, Frant, Maciej, additional, Lange, Christian E., additional, Dubchak, Inna, additional, Mezhenskyi, Andrii A., additional, Nychyk, Serhiy, additional, Bortz, Eric, additional, and Drown, Devin M., additional

Published
2019
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27. A multi gene-approach genotyping method identifies 24 genetic clusters within the genotype II-European African swine fever viruses circulating from 2007 to 2022

Author

Carmina Gallardo, Nadia Casado, Alejandro Soler, Igor Djadjovski, Laura Krivko, Encarnación Madueño, Raquel Nieto, Covadonga Perez, Alicia Simon, Emiliya Ivanova, Daniel Donescu, Vesna Milicevik, Eleni Chondrokouki, Imbi Nurmoja, Maciej Frant, Francesco Feliziani, Petr Václavek, Simona Pileviciene, Arias Marisa, European Commission, Gallardo, Carmina, Djadjovski, Igor, Krivko, Laura, Chondrokouki, Eleni, Frant, Maciej, and Pileviciene, Simona

Subjects
Genotyping, General Veterinary, SNP, TRS, ASFV, Genetic groups
Abstract

14 Pág., African swine fever (ASF) is a contagious viral disease of pigs and wild boar that poses a major threat to the global swine industry. The genotype II African swine fever virus (ASFV) entered the European Union (EU) in 2014 and since then fourteen countries have been affected, Italy and North Macedonia being the last in 2022. While whole genome sequencing remains the gold standard for the identification of new genetic markers, sequencing of multiple loci with significant variations could be used as a rapid and cost-effective alternative to track outbreaks and study disease evolution in endemic areas., This study was supported by the European Union Reference laboratory for ASF (grant no. UE- LR PPA/03).

Published
2023

28. Dynamics of African swine fever virus (ASFV) infection in domestic pigs infected with virulent, moderate virulent and attenuated genotype II ASFV European isolates

Author

Carmina Gallardo, Maciej Frant, Raquel Nieto, Grzegorz Woźniakowski, Covadonga Pérez, Imbi Nurmoja, Svetlana Cvetkova, Marisa Arias, A. Simón, Cristina Cano-Gómez, Alejandro Soler, Gallardo, Carmina, Cano-Gómez, Cristina, Cvetkova, Svetlana, Frant, Maciej, Woźniakowski, Grzegorz, Simón, Alicia, and Arias, Marisa

Subjects
Swine Diseases, Genotype, Virulence, General Veterinary, General Immunology and Microbiology, Swine, Transmission (medicine), Sus scrofa, Virus shedding, General Medicine, Disease, Biology, African Swine Fever Virus, Virology, Virus, Excretion, Immune system, Genotype II, Animals, Viral shedding, African Swine Fever
Abstract

This study aimed to compare the infection dynamics of three genotype II African swine fever viruses (ASFV) circulating in Europe. Eighteen domestic pigs divided into three groups were infected intramuscularly or by direct contact with two haemadsorbent ASFVs (HAD) from Poland (Pol16/DP/ OUT21) and Estonia (Est16/WB/Viru8), and with the Latvian non-HAD ASFV (Lv17/WB/Rie1). Parameters, such as symptoms, pathogenicity, and distribution of the virus in tissues, humoral immune response, and dissemination of the virus by blood, oropharyngeal and rectal routes, were investigated. The Polish ASFV caused a case of rapidly developing fatal acute disease, while the Estonian ASFV caused acute to sub-acute infections and two animals survived. In contrast, animals infected with the ASFV from Latvia developed a more subtle, mild, or even subclinical disease. Oral excretion was sporadic or even absent in the attenuated group, whereas in animals that developed an acute or sub-acute form of ASF, oral excretion began at the same time the ASFV was detected in the blood, or even 3 days earlier, and persisted up to 22 days. Regardless of virulence, blood was the main route of transmission of ASFV and infectious virus was isolated from persistently infected animals for at least 19 days in the attenuated group and up to 44 days in the group of moderate virulence. Rectal excretion was limited to the acute phase of infection. In terms of diagnostics, the ASFV genome was detected in contact pigs from oropharyngeal samples earlier than in blood, independently of virulence. Together with blood, both samples could allow to detect ASFV infection for a longer period. The results presented here provide quantitative data on the spread and excretion of ASFV strains of different virulence among domestic pigs that can help to better focus surveillance activities and, thus, increase the ability to detect ASF introductions earlier., This study was supported by the INIA (Projects RTA2015-00033-C02-01, AT2015-002) and the European Union Reference laboratory for ASF (Grant no.: UE- LR PPA/03). We would like to thank all the staff at the INIA-CISA who worked in the animal facilities department.

Published
2021

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