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2 results on '"Feline SCC"'

1. Transforming properties of Felis catus papillomavirus type 2 E6 and E7 putative oncogenes in vitro and their transcriptional activity in feline squamous cell carcinoma in vivo.

Author

Altamura, Gennaro, Corteggio, Annunziata, Pacini, Laura, Conte, Andrea, Pierantoni, Giovanna Maria, Tommasino, Massimo, Accardi, Rosita, and Borzacchiello, Giuseppe

Subjects
*VIRUS diseases, *SQUAMOUS cell carcinoma, *PAPILLOMAVIRUSES, *ONCOGENES, *GENETIC transcription, *APOPTOSIS, *VIRUSES, *CAT diseases
Abstract

Felis catus papillomavirus type 2 (FcaPV2) DNA is found in feline cutaneous squamous cell carcinomas (SCCs); however, its biological properties are still uncharacterized. In this study, we successfully expressed FcaPV2 E6 and E7 putative oncogenes in feline epithelial cells and demonstrated that FcaPV2 E6 binds to p53, impairing its protein level. In addition, E6 and E7 inhibited ultraviolet B (UVB)-triggered accumulation of p53, p21 and pro-apoptotic markers such as Cleaved Caspase3, Bax and Bak, suggesting a synergistic action of the virus with UV exposure in tumour pathogenesis. Furthermore, FcaPV2 E7 bound to feline pRb and impaired pRb levels, resulting in upregulation of the downstream pro-proliferative genes Cyclin A and Cdc2. Importantly, we demonstrated mRNA expression of FcaPV2 E2, E6 and E7 in feline SCC samples, strengthening the hypothesis of a causative role in the development of feline SCC. [ABSTRACT FROM AUTHOR]

Published
2016
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2. Transforming properties of Felis catus papillomavirus type 2 E6 and E7 putative oncogenes in vitro and their transcriptional activity in feline squamous cell carcinoma in vivo

Author

Annunziata Corteggio, Giovanna Maria Pierantoni, Andrea Conte, Massimo Tommasino, Gennaro Altamura, Giuseppe Borzacchiello, Laura Pacini, Rosita Accardi, Altamura, Gennaro, Corteggio, Annunziata, Pacini, Laura, Conte, Andrea, Pierantoni, GIOVANNA MARIA, Tommasino, Massimo, Accardi, Rosita, and Borzacchiello, Giuseppe

Subjects
Gene Expression Regulation, Viral, Transcriptional Activation, 0301 basic medicine, Felis catus papillomaviru, 040301 veterinary sciences, Papillomavirus E7 Proteins, Veterinary medicine, Cell, Cyclin A, Oncogenic viruse, Virus, Cell Line, 0403 veterinary science, Mice, 03 medical and health sciences, Downregulation and upregulation, Virology, medicine, Animals, RNA, Messenger, Papillomaviridae, Gene, E7, E6, Cyclin-dependent kinase 1, biology, Papillomavirus Infections, Oncogene Proteins, Viral, Feline oncology, 04 agricultural and veterinary sciences, Molecular biology, Veterinary virology, In vitro, 030104 developmental biology, medicine.anatomical_structure, Carcinoma, Squamous Cell, Cats, biology.protein, Carrier Proteins, Feline SCC, Oncovirus, Protein Binding, Signal Transduction
Abstract

Felis catus papillomavirus type 2 (FcaPV2) DNA is found in feline cutaneous squamous cell carcinomas (SCCs); however, its biological properties are still uncharacterized. In this study, we successfully expressed FcaPV2 E6 and E7 putative oncogenes in feline epithelial cells and demonstrated that FcaPV2 E6 binds to p53, impairing its protein level. In addition, E6 and E7 inhibited ultraviolet B (UVB)-triggered accumulation of p53, p21 and pro-apoptotic markers such as Cleaved Caspase3, Bax and Bak, suggesting a synergistic action of the virus with UV exposure in tumour pathogenesis. Furthermore, FcaPV2 E7 bound to feline pRb and impaired pRb levels, resulting in upregulation of the downstream pro-proliferative genes Cyclin A and Cdc2. Importantly, we demonstrated mRNA expression of FcaPV2 E2, E6 and E7 in feline SCC samples, strengthening the hypothesis of a causative role in the development of feline SCC.

Published
2016

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