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Donor human milk (DHM) from a milk bank is the recommended feeding method for preterm infants when the mothers own milk (MOM) is not available. Despite this recommendation, information on the possible contamination of donor human milk and its impact on infant health outcomes is poorly characterised. The aim of this systematic review is to assess contaminants present in DHM samples that preterm and critically ill infants consume. The data sources used include PubMed, EMBASE, CINAHL and Web of Science. A search of the data sources targeting DHM and its potential contaminants yielded 426 publications. Two reviewers (S. T. and D. L.) conducted title/abstract screening through Covidence software, and predetermined inclusion/exclusion criteria yielded 26 manuscripts. Contaminant types (bacterial, chemical, fungal, viral) and study details (e.g., type of bacteria identified, study setting) were extracted from each included study during full-text review. Primary contaminants in donor human milk included bacterial species and environmental pollutants. We found that bacterial contaminants were identified in 100% of the papers in which bacterial contamination was sought (16 papers) and 61.5% of the full data set (26 papers), with the most frequently identified genera being Staphylococcus (e.g., Staphylococcus aureus and coagulase-negative Staphylococcus) and Bacillus (e.g., Bacillus cereus). Chemical pollutants were discovered in 100% of the papers in which chemical contamination was sought (eight papers) and 30.8% of the full data set (26 papers). The most frequently identified chemical pollutants included perfluoroalkyl substances (six papers), toxic metal (one paper) and caffeine (one paper). Viral and fungal contamination were identified in one paper each. Our results highlight the importance of establishing standardisation in assessing DHM contamination and future studies are needed to clarify the impact of DHM contaminants on health outcomes.

Grape molasses (GM), produced from grapes, is a traditional Iranian food and is widely consumed in Iran. However, GM adulteration is among the most widespread illegitimate procedures involving contamination of food with foreign materials, such as adding sugar–water solution, date syrup, sugar beet syrup, and grape sauce. This study used stable carbon 13C/12C isotope ratio analysis method to detect adulteration of GM samples with glucose syrups (GS), fructose syrups (FS), and beet sugar syrups (BS) at the ratio of 0%, 10%, 30%, and 50% (by weight). Physicochemical properties of GM including °Brix, conductivity, specific gravity, pH, moisture content, ash content, hydroxymethyl furfural, sugar content, and rheological properties of samples were investigated. The δ13C isotope ratio of the GM was determined as −26.61%, that of the GS as −13.23%, that of the FS as −13.42%, and that of the BS as −16.58%. The δ13C isotope ratio increased by the addition of adulterant syrups to GM. The addition of each adulterant syrup had a different effect on the physicochemical parameters; however, the °Brix and specific gravity had a positive correlation with the δ13C isotope ratio results. The magnitudes of G' and G" increase with an increase in frequency representing the viscoelastic behavior of samples. The obtained results of this study suggest the use of δ13C isotope ratio method as a fast and accurate method to investigate the adulteration of grape molasses. [ABSTRACT FROM AUTHOR]

Heavy metal contamination and potential risks to human health related to consuming major food crops near Iringa Municipal dumpsite are presented. Soil samples, maize (Zea mays L.) and common beans (Phaseolus vulgaris L.) were collected downslope around the dumpsite during wet season. Soil samples were analyzed using Energy-Dispersive X-Ray Fluorescence (EDXRF) while crop (maize and beans) samples were analyzed using Flame Atomic Absorption Spectrophotometer (FAAS). The potential health risks associated with human exposure to heavy metals in maize and common beans was evaluated according to the method prescribed by the United States Environmental Protection Agency. The concentrations Cd (3.25–10.78 mg/kg), As (1.84–10.98 mg/kg) and Ni (71.74–55.10 mg/kg) in soil samples exceed the maximum allowable limits set by Tanzania Bureau of Standards (TBS). The average heavy metal concentration in food crops followed the trend Cd, As < Cr, Pb < Ni < Cu < Mn < Zn with Pb in maize (0.96 mg/kg) and common beans (0.81 mg/kg) above FAO/WHO regulatory standards of 0.3 mg/kg. This implied health concerns to consuming food crops grown in the vicinity of the dumpsite. Further, lifetime–incremental cancer risk (ILCR) assessment suggest that consuming maize and common beans from the study area could increase the risk of both cancer and non-cancerous diseases in both adults and children. However, the degree of ILCR in children was noticeably higher (1.0 × 10–2) than in adults (5.10. × 10–6) for Pb in common beans. Therefore sustainable waste management should be stressed and continuous monitoring of environments around dumpsites is crucial to mitigate the negative impacts on public and environments. [ABSTRACT FROM AUTHOR]

Helicobacteriosis is a common bacterial infection caused by Helicobacter pylori. It affects the stomach and small intestines, leading to inflammation. Bacteria can spread through contaminated food or water. This review explores the role of food in the transmission of H. pylori, drawing on research from the past three decades. People commonly acquire the infection during childhood, often from close family members. Crowded living conditions can also contribute to the spread. This review also discusses various risk factors and highlights the challenges of detecting H. pylori, particularly in its dormant form. Techniques like ribotyping and restriction fragment length polymorphism hold promise for tracing transmission routes, but more long-term studies are needed to account for potential confounding factors. [ABSTRACT FROM AUTHOR]

Background: The adulteration of milk is an important issue that needs to be addressed because of its deleterious effects on health. Detection of adulterants in milk can be done in laboratories or by purchasing adulteration detection kits. There are few home-based rapid tests to detect common milk adulterants, which are less known by our community. Objectives of this study were to estimate the proportion of milk adulteration and to identify common adulterants, to determine the association of milk adulteration with gastrointestinal and renal diseases; and also to validate rapid home-based tests by comparing its result with standard milk adulteration detection kit. Materials and Methods: A community-based study was conducted on 330 medical and paramedical staff. Collected milk samples during family visits were tested by standard milk adulteration detection tests and rapid home-based tests. Results: Among 330 tested milk samples, 233 samples were adulterated with one or more than one adulterant. The most common adulteration observed was the addition of water to milk, followed by detergent, urea, and neutralizer. The specificity of the rapid tests was found to be almost 100% in the detection of water and detergent in milk and accuracy was above 80%; with "substantial agreement" (kappa = 0.7) between rapid tests and standard tests on kappa statistics. Conclusion: The present study has identified a large extent of milk adulteration and common adulterants, which may have harmful effects on health. The utility of home-based rapid tests is clearly proven to detect common adulterants. [ABSTRACT FROM AUTHOR]

Bacillus spp. are foodborne pathogens important in food contamination, especially in dried milk products. They are associated with foodborne outbreaks, spoilage of dairy products (sweet curdling and bitterness), and bovine mastitis. This study investigated the contamination rate of Bacillus spp. in a total of 105 samples of the dried milk products kishk, Cerelac and whole milk powder, marketed in Assiut city, Upper Egypt. The identification of the Bacillus spp. strains was completed using conventional biochemical methods and PCR protocols for the confirmation of isolates. Bacillus spp. were isolated from 57 out of 105 (54%) [95% CI: 44.6-64.0%] of the examined samples at levels of up to 7.7 log10 cfu/g. The prevalence of Bacillus spp. was significantly (P<0.05) higher in kishk (74%) than in the other food categories with a mean count of 4.04±0.49 log10 cfu/g. Regarding the species of Bacillus detected, 39.05% of the examined samples contained B. cereus, 8.57% contained B. subtilis, 2.86%: B. pumilus, another 2.86%: B. megatrium and 0.95%: B. licheniformis. B. cereus was isolated from 18 (51.43%) of the kishk samples, 9 (25.71%) of the Cerelac samples and 14 (40%) of the whole milk powder samples. Out of the 41 B. cereus strains previously identified by biochemical tests, 19 (46.34%) isolates were also confirmed using PCR (61.11%, 42.86% and 22.22% for kishk, whole milk powder and Cerelac, respectively). In conclusion, incorporating preventive measures to reduce bacterial contamination in the Egyptian dairy environment are warranted in order to avoid the contamination of milk products with these life-threatening pathogens. [ABSTRACT FROM AUTHOR]

Staphylococcus aureus biofilm is a common bio-contaminant source that leads to food cross-contamination and foodborne disease outbreaks. Hence, there is a need for searching novel antibiofilm agents with potential anti-virulence properties to control S. aureus contamination and infections in food systems. In this study, the antibiofilm effects of lactobionic acid (LBA) against S. aureus and its influence on virulence were explored. The minimum inhibition concentration of LBA on S. aureus was 8 mg/mL. Viable count and crystal violet assays revealed that LBA inhibited and inactivated S. aureus biofilms. Microscopic observations further confirmed the antibiofilm activity of LBA on S. aureus that disrupted the biofilm architecture and inactivated the viable cells in biofilms. Moreover, LBA decreased the release of extracellular DNA (eDNA) and extracellular polysaccharide (EPS) in S. aureus biofilms. LBA suppressed biofilm formation by intervening metabolic activity and reduced virulence secretion by repressing the hemolytic activity of S. aureus. Furthermore, LBA altered the expressions of biofilm- and virulence-related genes in S. aureus, further confirming that LBA suppressed biofilm formation and reduced the virulence secretion of S. aureus. The results suggest that LBA might be useful in preventing and controlling biofilm formation and the virulence of S. aureus to ensure food safety. [ABSTRACT FROM AUTHOR]

Background: Sterigmatocystin (STE) is a mycotoxin widely found in contaminated food and foodstuffs, and excessive long-term exposure to STE is associated with several health issues, including infertility. However, there is little information available regarding the effects of STE toxin on the female reproductive system, particularly concerning oocyte maturation. Methods: In the present study, we investigated the toxic effects of STE on mouse oocyte maturation. We also used Western blot, immunofluorescence, and image quantification analyses to assess the impact of STE exposure on the oocyte maturation progression, mitochondrial distribution, oxidative stress, DNA damages, oocyte ferroptosis and asymmetric division defects. Results: Our results revealed that STE exposure disrupted mouse oocyte maturation progression. When we examined the cellular changes following 100 µM STE treatment, we found that STE adversely affected polar body extrusion and induced asymmetric division defects in oocytes. RNA-sequencing data showed that STE exposure affects the expression of several pathway-correlated genes during oocyte meiosis in mice, suggesting its toxicity to oocytes. Based on the RNA-seq data, we showed that STE exposure induced oxidative stress and caused DNA damage in oocytes. Besides, ferroptosis and α-tubulin acetylation were also found in STE-exposed oocytes. Moreover, we determined that STE exposure resulted in reduced RAF1 protein expression in mouse oocytes, and inhibition of RAF1 activity also causes defects in asymmetric division of mouse oocytes. Conclusions: Collectively, our research provides novel insights into the molecular mechanisms whereby STE contributes to abnormal meiosis. [ABSTRACT FROM AUTHOR]

Clostridium bacteria were already known to Hippocrates many years before Christ. The name of the Clostridium species is owed to the Polish microbiologist, Adam Prażmowski. It is now known that these Clostridium bacteria are widespread in the natural environment, and their presence in food products is a threat to human health and life. According to European Food Safety Authority (EFSA) reports, every year, there are poisonings or deaths due to ingestion of bacterial toxins, including those of the Clostridium spp. The strengthening of consumer health awareness has increased interest in consuming products with minimal processing in recent years, which has led to a need to develop new techniques to ensure the safety of microbiological food, including elimination of bacteria from the Clostridium genera. On the other hand, the high biochemical activity of Clostridium bacteria allows them to be used in the chemical, pharmaceutical, and medical industries. Awareness of microbiological food safety is very important for our health. Unfortunately, in 2022, an increase in infections with Clostridium bacteria found in food was recorded. Knowledge about food contamination should thus be widely disseminated. [ABSTRACT FROM AUTHOR]

Microbial contamination of food and alimentary toxoinfection/intoxication in humans are commonly caused by bacteria such as Salmonella spp., Escherichia coli, Yersinia spp., Campylobacter spp., Listeria monocytogenes, and fungi (Aspergillus, Fusarium). The addition of probiotic cultures (bacterial strains Lactobacillus and Bifidobacterium and the yeast Saccharomyces cerevisiae var. boulardii) to food contributes primarily to food enrichment and obtaining a functional product, but also to food preservation. Reducing the number of viable pathogenic microorganisms and eliminating or neutralizing their toxins in food is achieved by probiotic-produced antimicrobial substances such as organic acids (lactic acid, acetic acid, propionic acid, phenylacetic acid, and phenyllactic acid), fatty acids (linoleic acid, butyric acid, caproic acid, and caprylic acid), aromatic compounds (diacetyl, acetaldehyde, reuterin), hydrogen peroxide, cyclic dipeptides, bacteriocins, and salivabactin. This review summarizes the basic facts on microbial contamination and preservation of food and the potential of different probiotic strains and their metabolites (postbiotics), including the mechanisms of their antimicrobial action against various foodborne pathogens. Literature data on this topic over the last three decades was searched in the PubMed, Scopus, and Google Scholar databases, systematically presented, and critically discussed, with particular attention to the advantages and disadvantages of using probiotics and postbiotics as food biopreservatives. [ABSTRACT FROM AUTHOR]

The antimicrobial activity of an aqueous extract of the leaves of Schinus polygamus (cav.) Cabrera against microorganisms of food importance was evaluated. First, the leaf aqueous extract of Schinus polygamus was characterized, quantifying hydroxycinnamic acids and phenolic compounds. Then, a battery of strains was tested, including Escherichia coli ATCC 25922, Salmonella Typhimurium ATCC 14028, and Listeria monocytogenes ATCC 13932. Also, we tested wine spoilage yeasts such as Brettanomyces bruxellensis LAMAP2480, B. bruxellensis LAMAP1359, B. bruxellensis CECT1451, and Pichia guilliermondii NPCC1051. Tests were conducted using the kinetic curve of growth and cell viability counts. The results indicate that with 10% v/v of concentrated extract, it is possible to observe growth inhibition of all microorganisms studied, with statistically significant differences during the whole measurement time (70 h for bacteria and 145 h for yeast). [ABSTRACT FROM AUTHOR]

The exclusion mechanism of food contaminants such as bisphenol A (BPA), Flavonoids (FLA), and Goitrin (GOI) onto the novel gallium–metal organic framework (MOF) and functionalized MOF with oxalamide group (MOF-OX) is evaluated by utilizing molecular dynamics (MD) and Metadynamics simulations. The atoms in molecules (AIM) analysis detected different types of atomic interactions between contaminant molecules and substrates. To assess this procedure, a range of descriptors including interaction energies, root mean square displacement, radial distribution function (RDF), density, hydrogen bond count (HB), and contact numbers are examined across the simulation trajectories. The most important elements in the stability of the systems under examination are found to be stacking π–π and HB interactions. It was confirmed by a significant value of total interaction energy for BPA/MOF-OX (− 338.21 kJ mol−1) and BPA/MOF (− 389.95 kJ mol−1) complexes. Evaluation of interaction energies reveals that L–J interaction plays an essential role in the adsorption of food contaminants on the substrates. The free energy values for the stability systems of BPA/MOF and BPA/MOF-OX complexes at their global minima reached about BPA/MOF = − 254.29 kJ mol−1 and BPA/MOF-OX = − 187.62 kJ mol−1, respectively. Nevertheless, this work provides a new strategy for the preparation of a new hierarchical tree-dimensional of the Ga-MOF hybrid material for the adsorption and exclusion of food contaminates and their effect on human health. [ABSTRACT FROM AUTHOR]

Background: Hepatitis-E virus (HEV), an etiologic agent of acute inflammatory liver disease, is a significant cause of morbidity and mortality in South Asia. HEV is considered endemic in Nepal; but data on population-level infection transmission is sparse. Methods: We conducted a longitudinal serosurvey in central Nepal to assess HEV exposure. At each visit, capillary blood samples were collected and analyzed for the presence of anti-HEV IgG antibodies. The study took place between February 2019 and April 2021, with up to 4 visits per participant approximately 6 months apart. Results: We collected 2513 samples from 923 participants aged 0–25 years, finding a seroprevalence of 4.8% and a seroincidence rate of 10.9 per 1000 person-years. Young adults and individuals consuming surface water faced the highest incidence of infection. Geospatial analysis identified potential HEV clusters, suggesting a need for targeted interventions. Significance: Our findings demonstrate that HEV is endemic in Nepal and that the risk of infection increases with age. Author summary: Hepatitis-E virus (HEV) is an infectious cause of acute liver disease and is primarily transmitted through contaminated food and water. It has emerged as a leading cause of acute clinical hepatitis in South Asia. Pregnant women face the highest risk of complications and mortality. To better understand the impact of HEV in Nepal, we conducted a two-year cohort study in the Kathmandu Valley region. We collected dried blood spots from participants every 6 months and tested the samples to detect past and new infections of HEV. Our study, involving 923 individuals, revealed that 4.8% had IgG antibodies against HEV, indicating past exposure. Each year, about 11 in 1000 people between the ages of 1 and 25 years are newly exposed to the virus. The highest infection rates were among young adults and those using untreated surface water. Our study mapped the distribution of HEV, identifying specific areas with higher infection rates. [ABSTRACT FROM AUTHOR]

Food contamination is the result of over-reliance on fungicides in crop production. The presence of pesticide residues in agricultural commodities needs to be strategically solved by applying gentle agrotechnical procedures. The research aimed to evaluate the effect of the treatment of grapevine with fungicides on the content and mobility of 4 active substances in the leaves of the variety Hibernal. The experiment included a control variant treated with fungicides and an untreated variant. We focused on monitoring the active substances of fungicides used in the first half of the vegetation: azoxystrobin, cyflufenamid, difenoconazole, myclobutanil, and another 25 active substances, in terms of multi-residue analysis methodologies. Grape leaves for analysis were collected on two dates: in the phenological phase of berry development BBCH 73 and 14 days after grape harvest - BBCH 91. Samples were prepared according to STN EN 15662. For qualitative analyzes of residues, we used the QuEChERS method in combination with liquid (LC-MS/MS) and gas (GC-MS/MS) chromatography with tandem mass spectrometry. In the untreated variant, the contents of residues from both leaves collection were in undetectable values. In the leaf samples of the treated variant collected in BBCH 73, we established higher values of residues than the MRL determines, namely for azoxystrobin 0.083 mg/kg (MRL - 0.01 mg/kg). Azoxystrobin is the active substance of the Quadris max fungicide, and we applied this preparation 41 days before the first collection of leaves. In the analyzes of leaf sampling from the treated variant after grape harvest in BBCH 91, we found a decrease in the content of all monitored residues below detectable values. We consider the gradual degradation of residues in biological materials to be a very favorable result from the point of view of their impact on consumer health and the environment. Through visual observations of the vegetation, we found out that even the untreated variant had a perfect state of health. Hibernal is a variety suitable for biological production systems, and we assume that even in vintages with high pressure of fungal diseases, it is possible to maintain the health of the grapevine with ecological products. Using the appropriate grape variety combined with the right agrotechnical interventions makes it possible to use safer ecological practices to produce healthy food, maintaining sustainability and a healthy natural environment. The research will continue in the early Table grape varieties, where the vegetation period is shorter, and space for breaking down the residues is more difficult. [ABSTRACT FROM AUTHOR]

Mycotoxins are secondary metabolites produced during the growth, storage, and transportation of crops contaminated by fungi and are physiologically toxic to humans and animals. Aflatoxin, zearalenone, deoxynivalenol, ochratoxin, patulin, and fumonisin are the most common mycotoxins and can cause liver and nervous system damage, immune system suppression, and produce carcinogenic effects in humans and animals that have consumed contaminated food. Physical, chemical, and biological methods are generally used to detoxify mycotoxins. Although physical methods, such as heat treatment, irradiation, and adsorption, are fast and simple, they have associated problems including incomplete detoxification, limited applicability, and cause changes in food characteristics (e.g., nutritive value, organoleptic properties, and palatability). Chemical detoxification methods, such as ammonification, ozonation, and peroxidation, pollute the environment and produce food safety risks. In contrast, bioenzymatic methods are advantageous as they achieve selective detoxification and are environmentally friendly and reusable; thus, these methods are the most promising options for the detoxification of mycotoxins. This paper reviews recent research progress on common mycotoxins and the enzymatic principles and mechanisms for their detoxification, analyzes the toxicity of the degradation products and describes the challenges faced by researchers in carrying out enzymatic detoxification. In addition, the application of enzymatic detoxification in food and feed is discussed and future directions for the development of enzymatic detoxification methods are proposed for future in-depth study of enzymatic detoxification methods. [ABSTRACT FROM AUTHOR]

Food contamination poses a significant threat to public health, the economy, and human health worldwide, occurring at any stage of the food supply chain, from farm to fork. Efficient and effective real‐time monitoring methods for the early identification and rapid detection of pathogen contamination are critical to preventing possible food safety issues. In the past decade, flexible electrochemical biosensors have rapidly expanded in the detection of foodborne pathogens, owing to their ability to function well at biological interfaces that may be soft, intrinsically curvy, irregular, or deformable. The most important features of flexible sensors are their flexibility, multifunctionality, low cost, and good compatibility with solution processing methods. These properties enable flexible sensors to be used in various applications, including smart packaging. This review focuses on the materials, structures, and fabrication methods of flexible biosensors, as well as their applications in food safety monitoring. It examines recent advances in electrochemical immunosensors and aptasensors for pathogen detection, with a focus on their key components and their application in food analysis, while critically examining their current trends, particularly in conjunction with nanomaterials such as carbon nanotubes, graphene, gold nanoparticles, Indium tin oxide, etc. Finally, current issues and prospects for the future are discussed. [ABSTRACT FROM AUTHOR]

Phytochemicals-rich food-based botanicals including traditional or under-utilized plant-based ingredients can serve a dual functional role to help counter food contamination of bacterial origin, while also addressing the rise of diet-linked non-communicable chronic diseases (NCDs) such as type 2 diabetes, chronic hypertension and the associated oxidative stress. Hence the screening of these food-based botanicals for their phenolic content and profile, as well as antimicrobial, antioxidant, anti-hyperglycemic and anti-hypertensive properties has relevant merit. Using in vitro assay models, hot water extracts of different forms (slice, pickle, or powder) of amla (Phyllanthus emblica), clove (Syzygium aromaticum), kokum (Garcinia indica), and garlic (Allium sativum) were analyzed for their total soluble phenolic content (TSP) and phenolic profile as well as antimicrobial activity against strains of Salmonella Enteritidis, Listeria monocytogenes, and Escherichia coli that are associated with food-borne disease outbreaks. In addition, the antioxidant, anti-hyperglycemic and anti-hypertensive activity of the extracts were also determined using in vitro assay models, with the goal of establishing a dual functional role of the food safety and health protective benefits of these botanicals. A high baseline TSP content was observed in all the extracts and the major phenolic phytochemicals detected were gallic, cinnamic, ellagic, benzoic, dihydroxybenzoic, protocatechuic, and p-coumaric acid along with catechin and rutin. All extracts displayed significant antimicrobial activity against most of the bacterial strains tested and the antimicrobial activity was specific for each strain targeted in this study. Furthermore, significant antioxidant, anti-hyperglycemic and antihypertensive activity were observed among the botanical extracts, especially among the amla and kokum extracts. These results indicate that phytochemicals enriched botanicals, including amla and kokum, can be integrated into modern-day food preservation and dietary support strategies aimed at improving the food safety and health protective benefits of the food matrix. [ABSTRACT FROM AUTHOR]

Paper and board are widely used as food contact materials. For such sensitive applications, consumer safety regarding the transfer of chemical components and contaminants to the food needs to be established. Such safety assessments are becoming increasingly challenging not only due to intentionally added substances but also non-intentionally added substances. In the European Union, compliance testing and safety evaluation of paper in food contact are largely based on national legislation and standards. The underlying tests are conventional methods, often overestimating and sometimes underestimating the migration into food. In this article, the relevant standard test methods are contrasted with currently available scientific knowledge. The scientific approaches to develop and identify suitable test methods are critically reviewed. Furthermore, theoretical predictions via mathematical modeling, with the aim to realistically simulate transfer to food, are presented and discussed in comparison with available migration studies with foods. Objectives are to (i) summarize the actual scientific knowledge in the field and draw conclusions regarding the potential and limitations of the existing test methods and (ii) identify research gaps toward a better qualitative and quantitative understanding of transport processes of volatile and non-volatile substances from paper and board into foods. [ABSTRACT FROM AUTHOR]

The establishment of Listeria (L.) monocytogenes within food processing environments constitutes a significant public health concern. This versatile bacterium demonstrates an exceptional capacity to endure challenging environmental conditions in the food processing environment, where contamination of food products regularly occurs. The diverse repertoire of stress resistance genes, the potential to colonize biofilms, and the support of a co-existing microbiota have been proposed as root causes for the survival of L. monocytogenes in food processing environments. In this study, 71 sites were sampled after cleaning and disinfection in a European frozen vegetable processing facility, where L. monocytogenes in-house clones persisted for years. L. monocytogenes and L. innocua were detected by a culture-dependent method at 14 sampling sites, primarily on conveyor belts and associated parts. The presence of biofilms, as determined by the quantification of bacterial load and the analysis of extracellular matrix components (carbohydrates, proteins, extracellular DNA) was confirmed at nine sites (12.7%). In two cases, L. innocua was detected in a biofilm. Furthermore, we explored the resident microbial community in the processing environment and on biofilm-positive sites, as well as the co-occurrence of bacterial taxa with Listeria by 16S rRNA gene sequencing. Pseudomonas, Acinetobacter, and Exiguobacterium dominated the microbial community of the processing environment. Using differential abundance analysis, amplicon sequence variants (ASVs) assigned to Enterobacterales (Enterobacter, Serratia, unclassified Enterobacteriaceae) and Carnobacterium were found to be significantly higher abundant in Listeria-positive samples. Several Pseudomonas ASVs were less abundant in Listeria-positive compared to Listeria-negative samples. Acinetobacter, Pseudomonas, Janthinobacterium, Brevundimonas, and Exiguobacterium were key players in the microbial community in biofilms, and Exiguobacterium and Janthinobacterium were more relatively abundant in biofilms. Further, the microbial composition varied between the different areas and the surface materials. [ABSTRACT FROM AUTHOR]

Breast milk is one of the many distinct forms of food that can be contaminated with aflatoxin M1 (AFM1). They may be consumed by eating contaminated foods, such as contaminated meat and crops, which would then be present in breast milk and cause health problems, including nervous system disorders and cancers of the lungs, liver, kidneys, and urinary tract. However, the prevalently inconsistent explanation of prevalence and concentration remains a big challenge. Thus, this meta-analysis was conducted to determine the prevalence and concentration of harmful chemicals in breast milk in an African context. The databases MEDLINE, PubMed, Embase, SCOPUS, Web of Science, and Google Scholar were searched for both published and unpublished research. To conduct the analysis, the collected data were exported to Stata version 18. The results were shown using a forest plot and a prevalence with a 95% confidence interval (CI) using the random-effects model. The Cochrane chi-square (I2) statistics were used to measure the studies' heterogeneity, and Egger's intercept was used to measure publication bias. This review included twenty-eight studies with 4016 breast milk samples and newborns. The analysis showed the overall prevalence and concentration of aflatoxin M1 in breast milk were 53% (95% CI 40, 65; i2 = 98.26%; P = 0.001). The pooled mean aflatoxin M1 concentration in breast milk was 93.02 ng/l. According to this study, the eastern region of Africa was 62% (95% CI 39–82) profoundly affected as compared to other regions of the continent. In subgroup analysis by publication year, the highest level of exposure to aflatoxins (68%; 95% CI 47–85) was observed among studies published from 2010 to 2019. This finding confirmed that more than half of lactating women's breast milk was contaminated with aflatoxin M1 in Africa. The pooled mean aflatoxin M1 concentration in breast milk was 93.02 ng/l. According to this study, the eastern region of Africa was profoundly affected compared with other regions. Thus, the government and all stakeholders must instigate policies that mitigate the toxicity of aflatoxins in lactating women, fetuses, and newborns. [ABSTRACT FROM AUTHOR]

Today, plastic pollution is one of the biggest threats to the environment and public health. In the tissues of exposed species, micro- and nano-fragments accumulate, leading to genotoxicity, altered metabolism, and decreased lifespan. A model to investigate the genotoxic and tumor-promoting potential of nanoplastics (NPs) is Drosophila melanogaster. Here we tested polystyrene, which is commonly used in food packaging, is not well recycled, and makes up at least 30% of landfills. In order to investigate the biological effects and carcinogenic potential of 100 µm polystyrene nanoparticles (PSNPs), we raised Oregon [R] wild-type flies on contaminated food. After prolonged exposure, fluorescent PSNPs accumulated in the gut and fat bodies. Furthermore, PSNP-fed flies showed considerable alterations in weight, developmental time, and lifespan, as well as a compromised ability to recover from starvation. Additionally, we noticed a decrease in motor activity in DNAlig4 mutants fed with PSNPs, which are known to be susceptible to dietary stressors. A qPCR molecular investigation of the larval intestines revealed a markedly elevated expression of the genes drice and p53, suggesting a response to cell damage. Lastly, we used warts-defective mutants to assess the carcinogenic potential of PSNPs and discovered that exposed flies had more aberrant masses than untreated ones. In summary, our findings support the notion that ingested nanopolystyrene triggers metabolic and genetic modifications in the exposed organisms, eventually delaying development and accelerating death and disease. [ABSTRACT FROM AUTHOR]

Agricultural food commodities are highly susceptible to contamination by fungi and mycotoxins, which cause great economic losses and threaten public health. New technologies such as gamma ray irradiation, ultraviolet radiation, electron beam irradiation, microwave irradiation, pulsed light, pulsed electric fields, plasma, ozone, etc. can solve the problem of fungal and mycotoxin contamination which cannot be effectively solved by traditional food processing methods. This paper summarizes recent advancements in emerging food decontamination technologies used to control various fungi and their associated toxin contamination in food. It discusses the problems and challenges faced by the various methods currently used to control mycotoxins, looks forward to the new trends in the development of mycotoxin degradation methods in the future food industry, and proposes new research directions. [ABSTRACT FROM AUTHOR]

Introduction: Approximately 70% of diarrheal cases in Kenya are attributed to ingestion of contaminated food and water and costs an estimated $ 1 billion USD due to morbidity and cost of treatment. This study aimed to assess the levels of microbiological contamination of meat sold in selected butcheries in Nairobi and the handling practices of butcher shop attendants. Methods: A cross-sectional study design was used during which 200 meat samples were collected, and meat handling practices were observed. Total coliforms and Escherichia coli were enumerated using 3M™ Petrifilm® count plates. Additionally, quantification of tetracycline- and cefotaxime-resistant Enterobacteriaceae was done on agar plates containing the respective antibiotics. Bacterial species were confirmed by Matrix-Assisted Laser Desorption/Ionization-Time of Flight mass spectrometry. Results and discussion: Seventy two percent and 84% of the samples had E. coli and total coliforms respectively above the acceptable regulatory limits (i.e. E. coli >100 CFU/g, Total coliforms >361 CFU/g,) respectively as per the Kenya Bureau of Standards South African microbiological standards the European Union. Enterobacteriaceae resistant to tetracycline and cefotaxime were detected in 35% and 9.5% of the samples respectively. Eighty-five percent of the butcher shop attendants neither washed their hands before nor after handling the meat, 91% handled money while selling meat concurrently, and 99% did not wear gloves while handling meat. These poor meat handling practices coupled with the presence of microbial loads above the regulatory acceptable limits imply an increased risk of foodborne illness to consumers. Therefore, there is an urgent need for education of butcher shop attendants on appropriate handling of meat, highlighting the importance of good hygienic practices and their relationship to food safety, and provision of incentives for behavior change. This study is important and serves to inform policymakers in the identification of key control points for designing meat safety intervention(s). [ABSTRACT FROM AUTHOR]

The escalating incidence of foodborne salmonellosis poses a significant global threat to food safety and public health. As antibiotic resistance in Salmonella continues to rise, there is growing interest in bacteriophages as potential alternatives. In this study, we isolated, characterized, and evaluated the biocontrol efficacy of lytic phage L223 in chicken meat. Phage L223 demonstrated robust stability across a broad range of temperatures (20–70 °C) and pH levels (2–11) and exhibited a restricted host range targeting Salmonella spp., notably Salmonella Typhimurium and Salmonella Enteritidis. Characterization of L223 revealed a short latent period of 30 min and a substantial burst size of 515 PFU/cell. Genomic analysis classified L223 within the Caudoviricetes class, Guernseyvirinae subfamily and Jerseyvirus genus, with a dsDNA genome size of 44,321 bp and 47.9% GC content, featuring 72 coding sequences devoid of antimicrobial resistance, virulence factors, toxins, and tRNA genes. Application of L223 significantly (p < 0.005) reduced Salmonella Typhimurium ATCC 14,028 counts by 1.24, 2.17, and 1.55 log CFU/piece after 2, 4, and 6 h of incubation, respectively, in experimentally contaminated chicken breast samples. These findings highlight the potential of Salmonella phage L223 as a promising biocontrol agent for mitigating Salmonella contamination in food products, emphasizing its relevance for enhancing food safety protocols. [ABSTRACT FROM AUTHOR]

Background: According to a report by the World Health Organization (WHO) in 2015, approximately 600 million individuals, equating to nearly one-tenth of the global population, become sick due to consuming contaminated food each year. Furthermore, the report indicates that 420,000 individuals perish annually as a result, leading to the loss of 33 million healthy life years (DALYs). Among these cases, 40% of the burden of foodborne diseases affects children under the age of five, resulting in 125,000 deaths annually. Objectives: To distinguish the attitudes towards food safety practices in urban and rural households based on the WHO Five Keys. Materials and Methods: A cross-sectional study was conducted in Urban and rural households located in the field practice areas of Jawaharlal Nehru medical college in Belagavi Taluka, and District of Karnataka State. The study took place from January 1, 2017, to December 31, 2017. Results: In urban participants, 30.0% were aged 25 to 35 years whereas for rural participants, 35.8% were aged 18 to 25 years. The mean age for urban participants was 37.96 ± 14.9 years, and for rural participants, it was 33.48 ± 13.5 years. The difference in mean ages between urban and rural participants was statistically significant (χ2 = 39.53, p < 0.001). According to Modified B. G. Prasad's Classification of socio-economic status of urban participants, 34.3% of study participants belonged to Class IV whereas in rural participants, 41.3% in Class IV, differences in socio-economic status between urban and rural participants were statistically significant (χ2 = 59.61, p < 0.001). Conclusion: The study underscores the importance of recognizing and addressing the distinctive demographic characteristics between urban and rural populations, which can have implications for healthcare, education, and socioeconomic interventions tailored to the specific needs of each community. [ABSTRACT FROM AUTHOR]

Shiga toxin-producing Escherichia coli (STEC) causes a wide spectrum of diseases including hemorrhagic colitis and hemolytic uremic syndrome (HUS). Previously, we developed a rapid, sensitive, and potentially portable assay that identified STEC by detecting Shiga toxin (Stx) using a B-cell based biosensor platform. We applied this assay to detect Stx2 present in food samples that have been implicated in previous STEC foodborne outbreaks (milk, lettuce, and beef). The STEC enrichment medium, modified Tryptone Soy Broth (mTSB), inhibited the biosensor assay, but dilution with the assay buffer relieved this effect. Results with Stx2a toxoid-spiked food samples indicated an estimated limit of detection (LOD) of ≈4 ng/mL. When this assay was applied to food samples inoculated with STEC, it was able to detect 0.4 CFU/g or 0.4 CFU/mL of STEC at 16 h post incubation (hpi) in an enrichment medium containing mitomycin C. Importantly, this assay was even able to detect STEC strains that were high expressors of Stx2 at 8 hpi. These results indicate that the STEC CANARY biosensor assay is a rapid and sensitive assay applicable for detection of STEC contamination in food with minimal sample processing that can complement the current Food Safety Inspection Service (US) methodologies for STEC. [ABSTRACT FROM AUTHOR]

BACKGROUND: Microplastics are a pervasive contaminant cycling through food webs—leading to concerns regarding exposure and risk to humans. OBJECTIVES: We aimed to quantify and characterize anthropogenic particle contamination (including microplastics) in fish caught for human consumption from the Humber Bay region of Lake Ontario. We related quantities of anthropogenic particles to other factors (e.g., fish size) that may help in understanding accumulation of microplastics in fish. METHODS: A total of 45 samples of six fish species collected from Humber Bay in Lake Ontario near Toronto, Ontario, Canada, were examined for anthropogenic particles in their gastrointestinal (GI) tracts and fillets. Using microscopy and spectroscopy, suspected anthropogenic particles were identified and characterized. RESULTS: We observed anthropogenic particles in the GI tracts and fillets of all species. Individual fish had a mean±standard deviation of 138±231 anthropogenic particles, with a single fish containing up to 1,508 particles. GI tracts had 93±226 particles/fish (9.8±32.6 particles/gram), and fillets had 56±61 particles/fish (0.5±0.8 particles/gram). Based on a consumption rate of 2 servings/week, the average yearly human exposure through the consumption of these fish fillets would be 12,800±18,300 particles. DISCUSSION: Our findings suggest that consumption of recreationally caught freshwater fish can be a pathway for human exposure to microplastics. The elevated number of particles observed in fish from Humber Bay highlights the need for large-scale geographic monitoring, especially near sources of microplastics. Currently, it is unclear what the effects of ingesting microplastics are for humans, but given that recreationally caught freshwater fish are one pathway for human exposure, these data can be incorporated into future human health risk assessment frameworks for microplastics. [ABSTRACT FROM AUTHOR]

Background: Sarcocystis is a food‐borne zoonotic protozoan whose final hosts are humans, dogs, cats, and other carnivores and intermediate hosts are birds and mammals, especially humans and herbivores. Humans become infected by eating raw and undercooked meat contaminated with bradyzoites or by consuming water or food contaminated with the sporocyst stage of the parasite. Objectives: The aim of this study was to investigate the effects of gamma radiation and electron beam on the survival rate of Sarcocystis bradyzoites in infected beef and to determine the effective dose. Methods: Three replicates of 100 g of infected meat were treated with different doses (0.5, 1, 1.5 and 2 kGy). As a control, 20 g of contaminated meat was stored separately at 4°C. The viability of the bradyzoites after digestion in pepsin solution was assessed, stained (trypan blue) and unstained, under a stereomicroscope. To assess survival of the bradyzoites, the irradiated meat samples were fed to 30 dogs. After 10 days, faecal samples were examined for sporocysts. Results: The results showed that the highest and lowest mortality rate of Sarcocystis bradyzoites in infected organs using electron beam at a dose of 2 kGy were 92.5% and 100%, respectively, and the lowest mortality rate at a dose of 0.5 kGy were 2.5% and 7.89%, respectively. Conclusion: The results of statistical analysis showed that the mortality rate of Sarcocystis bradyzoites was significant between different doses of gamma ray and electron beam, so that gamma rays were better compared to electron beam in destroying Sarcocystis bradyzoites. [ABSTRACT FROM AUTHOR]

Anthropogenic activities have contributed to the increase of heavy metals and to the alteration of their natural cycles in the environment. Mercury (Hg) is now considered to be one of the most toxic elements whose levels need to be monitored in abiogenic and biogenic environmental compartments. It can enter the body of animal in several ways, mainly through contaminated food. In this study, we compared mercury levels in mouse hair and songbird feathers from Zhongar Alatau National Park in Kazakhstan and from national parks in Slovakia. We sampled mice of the genus Apodemus and songbirds of three genera – Parus, Phylloscopus and Turdus. Total mercury concentrations were measured using DMA-80. The results showed higher levels of Hg bioaccumulation in Slovakia than in Kazakhstan in both songbirds and mice. The three songbird species from Kazakhstan showed differences due to different feeding niches. High levels were found in thrushes, which are considered insectivorous ground feeders, whereas the lowest mercury concentrations were detected in tits, which are considered more generalist feeders. In Slovakia, the trend was different, with tits having similar levels of Hg to thrushes, a reflection of the different levels of contamination of environmental components. Mice showed overall lower concentrations than birds at both sites. [ABSTRACT FROM AUTHOR]

Oral and dental hygiene plays an important role in the health and well-being of our bodies. Failure to take basic oral and dental hygiene measures not only damages the health of teeth and gums, but can also significantly increase the risk of heart disease, cancer, and diabetes. Entamoeba gingivalis and Trichomonas tenax are reported to be anaerobic parasites found in the human mouth. These parasites are transmitted through saliva, contaminated food containers, drinking water, and/or other utensils. Since the observance of personal hygiene and discipline in taking care of oral and dental health is less in children and this affects their performance in school and their future success, we aimed to evaluate a parasitological and molecular survey of E. gingivalis and T. tenax among children (2 to 15 years) in Lorestan Province, in western Iran. This descriptive cross-sectional survey was conducted from October 2021 to October 2022 on 660 children (aged 2-15 years) referred to health centers of Lorestan Province, Iran. The total prevalence of the parasites was 108 (16.4%) and 117 (17.7%) by microscopy and PCR, respectively. No significant association was reported among age (P=0.716), gender (P=0.289), parental education (P=0.812), tooth brushing (P=0.170), and prevalence of these parasites in children. Conversely, a significant association was reported between settling in rural areas (P=0.002), mouthwash (P=0.001), and the prevalence of these parasites in children. By multivariate test, settling in rural districts (P=0.007) and mouthwash (P<0.001) were considerably associated with the rate of these parasites. We found a significant prevalence of these parasites in children in Lorestan Province, western Iran, indicating that dentists as well as children's parents should pay special attention to oral health strategies in children. [ABSTRACT FROM AUTHOR]

Background and objective: This study investigated the protective effects of kaempferol, a natural flavonoid known for its antioxidant properties, against ochratoxin A-induced renal damage. Ochratoxin A, a nephrotoxic mycotoxin found in contaminated food and feed, primarily induces renal damage through oxidative stress. The objective was to assess kaempferol’s potential in mitigating ochratoxin A -induced renal toxicity and its underlying molecular mechanisms. Methods: Male albino mice were divided into four groups: normal control, kaempferol control, ochratoxin A -induced renal toxicity, and ochratoxin A -induced renal toxicity treated with kaempferol. Ochratoxin A was administered twice a week for 8 weeks at 5 mg/kg body weight to induce renal toxicity. Kaempferol (50 mg/kg) was administered twice a week for 8 weeks after a 4-hour interval following ochratoxin A administration. Results: Results showed that kaempferol normalized levels of blood urea nitrogen (BUN) and creatinine, indicating protection against ochratoxin A-induced pathophysiology. Kaempferol also increased phosphorylation of PI3K and AKT, potentially activating antioxidative and anti-apoptotic signaling pathways regulated by Nrf2. Additionally, kaempferol enhanced Bcl-2 activation while suppressing caspase-3, suggesting anti-apoptotic effects. Histopathological analysis revealed reduced renal damage in kaempferol-treated mice compared to those exposed to ochratoxin A alone. Conclusion: Kaempferol demonstrated efficacy in preventing ochratoxin A -induced kidney injury through antioxidative and anti-apoptotic mechanisms. These findings suggest potential therapeutic applications of kaempferol in oxidative stress-induced renal damage. [ABSTRACT FROM AUTHOR]

Due to the growing concerns surrounding microbial contamination and food safety, there has been a surge of interest in fabricating novel food packaging with highly efficient antibacterial activity. Herein, we describe novel photodynamic antibacterial konjac glucomannan (KGM)/polyvinylpyrrolidone (PVP) nanofibers incorporated with lignin-zinc oxide composite nanoparticles (L-ZnONPs) and curcumin (Cur) via electrospinning technology. The resulting KGM/PVP/Cur/L-ZnONPs nanofibers exhibited favorable hydrophobic properties (water contact angle: 118.1°), thermal stability, and flexibility (elongation at break: 241.9%). Notably, the inclusion of L-ZnONPs and Cur endowed the nanofibers with remarkable antioxidant (ABTS radical scavenging activity: 98.1%) and photodynamic antimicrobial properties, demonstrating enhanced inhibitory effect against both Staphylococcus aureus (inhibition: 12.4 mm) and Escherichia coli (12.1 mm). As a proof-of-concept study, we evaluated the feasibility of applying nanofibers to fresh strawberries, and the findings demonstrated that our nanofibers could delay strawberry spoilage and inhibit microbial growth. This photodynamic antimicrobial approach holds promise for design of highly efficient antibacterial food packaging, thereby contributing to enhanced food safety and quality assurance. [ABSTRACT FROM AUTHOR]

Polycyclic aromatic hydrocarbons (PAHs) represent a category of persistent organic pollutants that pose a global concern in the realm of food safety due to their recognized carcinogenic properties in humans. Food can be contaminated with PAHs that are present in water, air, or soil, or during food processing and cooking. The wide and varied sources of PAHs contribute to their persistent contamination of food, leading to their accumulation within these products. As a result, monitoring of the levels of PAHs in food is necessary to guarantee the safety of food products as well as the public health. This review paper attempts to give its readers an overview of the impact of PAHs on crops, their occurrence and sources, and the methodologies employed for the sample preparation and detection of PAHs in food. In addition, possible directions for future research are proposed. The objective is to provide references for the monitoring, prevention, and in-depth exploration of PAHs in food. [ABSTRACT FROM AUTHOR]

This document is a summary of a special issue of the journal Foods, titled "Risk Assessment of Microbiological and Chemical Hazards in Foods." The issue focuses on the importance of food safety and the risks associated with chemical and biological contaminants in food. It covers topics such as the presence of toxic metals and metalloids in pig and frog meat, fungal contamination in spices, chemical contaminants in food processing, the presence of phthalate esters in coffee, and the detection of foodborne pathogens in seafood. The issue also includes reviews on microplastics in the food chain and the association between dietary acrylamide exposure and cancer risk. Overall, the special issue aims to promote research and collaboration in addressing foodborne risks and ensuring food safety. [Extracted from the article]

The Arcobacteraceae bacterial family includes species isolated from animals and related food products. Moreover, these species have been found in other ecological niches, including water. Some species, particularly Arcobacter butzleri and Arcobacter cryaerophilus, have been isolated from human clinical cases and linked to gastrointestinal symptoms. The presence of antibiotic-resistant strains is a concern for public health, considering the possible zoonoses and foodborne infections caused by contaminated food containing bacteria resistant to antibiotic treatments. This review aims to highlight the importance of antibiotic resistance in Arcobacter spp. isolates from several sources, including information about antibiotic classes to which this bacterium has shown resistance. Arcobacter spp. demonstrated a wide spectrum of antibiotic resistance, including several antibiotic resistance genes. Antibiotic resistance genomic traits include efflux pumps and mutations in antibiotic target proteins. The literature shows a high proportion of Arcobacter spp. that are multidrug-resistant. However, studies in the literature have primarily focused on the evaluation of antibiotic resistance in A. butzleri and A. cryaerophilus, as these species are frequently isolated from various sources. These aspects underline the necessity of studies focused on several Arcobacter species that could potentially be isolated from several sources. [ABSTRACT FROM AUTHOR]

Knowledge of the microbiota present in food processing environments is a significant advance that will allow for better evaluation of the risk of food contamination and a better design of the procedures for sanitization. The levels of microbial group indicators of hygienic quality were determined in different areas of the slaughter lines of two poultry slaughterhouses in the northwest of Spain (22 surfaces in each slaughterhouse were studied). The average microbial levels (cfu/cm2) were 2.15 × 102 ± 4.26 × 102 (total aerobic counts, TAC), 1.99 × 102 ± 5.00 × 102 (psychrotrophic microorganisms), 3.10 × 100 ± 1.37 × 101 (enterobacteria), 3.96 × 100 ± 2.55 × 101 (coliforms), 1.80 × 10−1 ± 7.79 × 10−1 (enterococci), and 1.12 × 10−1 ± 3.35 × 10−1 (vancomycin-resistant enterococci, VRE). TAC and psychrotrophic microorganisms were the most abundant groups in all samples (p < 0.05). The counts of both microbial groups were higher (p < 0.05) in samples of Slaughterhouse A than in those of Slaughterhouse B. Microbial loads for the rest of the bacteria were not influenced by the slaughterhouse sampled (p > 0.05). All 44 samples showed TAC and psychrotrophic microorganisms. Colonies of the rest of the microbial groups were only found in 26 samples (59.1% of the total). The isolates (one from each sample) were identified with MALDI-TOF and PCR. Gram-negative bacteria (all Enterobacteriaceae) were isolated in 23 samples, and Gram-positive bacteria were isolated in 16 (9 Enterococcus spp., 2 Enterococcus spp. and VRE, 3 VRE, 1 Enterococcus spp. and Listeria spp., and 1 Listeria spp.). The resistance of the strains to 11 (Enterococcus spp.) or 17 (Enterobacteriaceae) antibiotics was determined (disk diffusion, CLSI), finding an average of 2.05 ± 2.06 resistances per strain (3.46 ± 2.27 if reduced susceptibility reactions are included). A total of 37.3% of the Enterobacteriaceae isolates had a gene for resistance to beta-lactam antibiotics (blaTEM, blaCTX-M-15, blaKPC, blaCMY-2 or blaNDM). The high prevalence of resistant bacteria and resistance genes highlights the need to establish measures to control the spread of antibiotic resistance in poultry slaughterhouses. The findings of this work could contribute to the design of more effective sanitation procedures. [ABSTRACT FROM AUTHOR]

The ability of Salmonella species to adhere to surfaces and form biofilms, leading to persistent environmental reservoirs, might represent a direct link between environmental contamination and food processing contamination. The purpose of this study was to investigate the biofilm-forming ability of 80 multidrug-resistant (MDR) and extended-spectrum beta-lactamase (ESBL) producing Salmonella enterica serovar Infantis strains isolated from the broiler food chain production through whole genome sequencing (WGS), PCR, and morphotype association assays. Biofilm formation was quantified by testing the strains at two different temperatures, using 96-well polystyrene plates. The rough and dry colony (rdar) morphotype was assessed visually on Congo red agar (CRA) plates. Based on our results, all tested S. Infantis strains produced biofilm at 22 °C with an rdar morphotype, while at 37 °C, all the isolates tested negative, except one positive. Most isolates (58.75%) exhibited strong biofilm production, while 36.25% showed moderate production. Only 5 out of 80 (6.25%) were weak biofilm producers. WGS analysis showed the presence of the fim cluster (fimADF) and the csg cluster (csgBAC and csgDEFG), also described in S. Typhimurium, which are responsible for fimbriae production. PCR demonstrated the presence of csgD, csgB, and fimA in all 80 S. Infantis strains. To our knowledge, this is the first study comparing the effects of two different temperatures on the biofilm formation capacity of ESBL producing S. Infantis from the broiler production chain. This study highlights that the initial biofilm components, such as curli and cellulose, are specifically expressed at lower temperatures. It is important to emphasize that within the broiler farm, the environmental temperature ranges between 18–22 °C, which is the optimum temperature for in vitro biofilm formation by Salmonella spp. This temperature range facilitates the expression of biofilm-associated genes, contributing to the persistence of S. Infantis in the environment. This complicates biosecurity measures and makes disinfection protocols on the farm and in the production chain more difficult, posing serious public health concerns. [ABSTRACT FROM AUTHOR]

Background: The presence of aflatoxin M1, a toxic, mutagenic, and carcinogenic substance, poses significant health risks. Hence, this study assesses the levels of aflatoxin M1 in raw, pasteurized, and sterilized milk obtained from the North Khorasan Province, Iran, and evaluates its potential impact on consumer health. Methods: This descriptive-analytical study randomly collected 189 raw milk samples. Additionally, 70 pasteurized and sterilized milk samples from supermarkets in the North Khorasan Province, Iran, were included. All samples were assessed for aflatoxin M1 contamination using the enzyme-linked immunosorbent assay method. Meanwhile, statistical analysis (analysis of variance) was performed on the results. Results: The Mean±SD concentration of aflatoxin M1 in raw milk from the North Khorasan Province, Iran, was 18.44±34.6 ng/L, while pasteurized and sterilized milk had a higher mean concentration of 42.8±21.54 ng/L. The lowest concentration was found in Bojnord City, Iran, at 9.30±8.91 ng/L, while the highest concentration was detected in Jajarm at 30.70±80.50 ng/L. The mean contamination of aflatoxin M1 was not statistically significant (P=0.42) in most cities of the province. Meanwhile, 6.34% of raw milk and 11.4% of pasteurized milk samples had higher levels of aflatoxin M1 than the maximum limit recommended by the Iran National Standard Organization (INSO) (100 ng/L). However, none of the milk samples exceeded the permissible limit set by the Veterinary Organization of the country (500 ng/L). Conclusion: The mean concentration of aflatoxin M1 in raw and pasteurized milk from the North Khorasan Province is lower than the European Union (EU) standard of 50 ng/L and the approved limit set by the country’s Veterinary Organization (500 ng/L). Therefore, it is not considered a significant threat to the health of adults. [ABSTRACT FROM AUTHOR]

Microorganisms have significant potential to control fungal contamination in various foods. However, the identification of strains that exhibit robust antifungal activity poses challenges due to highly context-dependent responses. Therefore, to fully exploit the potential of isolates as antifungal agents, it is crucial to systematically evaluate them in a variety of biotic and abiotic contexts. Here, we present an adaptable and scalable method using a robotic platform to study the properties of 1022 isolates obtained from maple sap. We tested the antifungal activity of isolates alone or in pairs on M17 + lactose (LM17), plate count agar (PCA), and sucrose–allantoin (SALN) culture media against Kluyveromyces lactis, Candida boidinii, and Saccharomyces cerevisiae. Microorganisms exhibited less often antifungal activity on SALN and PCA than LM17, suggesting that the latter is a better screening medium. We also analyzed the results of ecological interactions between pairs. Isolates that showed consistent competitive behaviors were more likely to show antifungal activity than expected by chance. However, co-culture rarely improved antifungal activity. In fact, an interaction-mediated suppression of activity was more prevalent in our dataset. These findings highlight the importance of incorporating both biotic and abiotic factors into systematic screening designs for the bioprospection of microorganisms with environmentally robust antifungal activity. [ABSTRACT FROM AUTHOR]

Each year in the United States, >800 foodborne outbreaks are reported, causing >14,000 illnesses and >800 hospitalizations (1-3). Foodborne outbreaks range from small, localized outbreaks, such as those associated with [...]

Microbiological contamination of food poses a significant risk to public health, as a popular ready-to-eat food in the Middle-East, falafel sandwiches require no processing. When ingested, their microbiological integrity is extremely important to the population's health. The aim of the present research was to evaluate the microbial load of falafel sandwich and its basic components, which is an important indicator of hygiene and safety; to that effect, we tested 120 samples from different restaurants in Amman, Jordan (30 falafel sandwiches, 30 tahini salad, 30 hummus, and 30 falafel). The collected samples were transferred to the laboratory in the ice box and tested for microbiological and chemical analysis (pH and titratable acidity). Appropriate media were used in the enumeration: Plate Count Agar, De Man, Rogosa, and Sharpe agar (MRS), Violet Red Bile Lactose Agar, and Baird-Parker Agar for mesophilic aerobes, lactic acid bacteria, coliforms, and Staphylococcus aureus, respectively. In all samples, the average pH was [greater than or equal to] 5 and the average titratable acidity (as citric acid) was [greater than or equal to] 0.55%, thus permitting the growth of many microorganisms. The samples were assessed for aerobic plate count (APC) and the counts of coliforms (CC), lactic acid bacteria (LABC), yeasts and molds (YMC), Staphylococcus aureus count (S. aureus), as well as for the presence of Salmonella enterica (S. enterica) and Listeria monocytogenes (L. monocytogenes). Average APC of 'falafel' sandwich, 'tahini' salad, 'hummus', and falafel were 6.4, 6.3, 5.8, and 2.9 [log.sub.10] CFU/g respectively; average CC was 2.3, 2.8,1.9, and 0.6 [log.sub.10] CFU/g, respectively; average LABC was 5.6, 5.5, 5.3, and 2.4 [log.sub.10] CFU/g, respectively; average YMC was 4.2, 3.8, 3, and 0.7 [log.sub.10] CFU/g, respectively; average S. aureus was 2.09,1.68,0, and 0.3 [log.sub.10] CFU/g, respectively. S. enterica and L. monocytogenes were not isolated from any sample. This might be due of the exposure to high temperatures during the frying process, 'falafel' samples had the lowest microbial load. The study revealed through these microbial counts, that hummus and tahini salad are most likely to introduce microorganisms to falafel sandwich. Key words: falafel sandwich, tahini salad, hummus, coliforms, lactic acid bacteria, INTRODUCTION Ready-to-eat food (RTF) is a meal or part of a meal sold to the consumer immediately or later to be directly consumed and does not require further processing, such [...]

Background: The presence of aflatoxin M1, a toxic, mutagenic, and carcinogenic substance, poses significant health risks. Hence, this study assesses the levels of aflatoxin M1 in raw, pasteurized, and sterilized milk obtained from the North Khorasan Province, Iran, and evaluates its potential impact on consumer health. Methods: This descriptive-analytical study randomly collected 189 raw milk samples. Additionally, 70 pasteurized and sterilized milk samples from supermarkets in the North Khorasan Province, Iran, were included. All samples were assessed for aflatoxin M1 contamination using the enzyme-linked immunosorbent assay method. Meanwhile, statistical analysis (analysis of variance) was performed on the results. Results: The Mean±SD concentration of aflatoxin M1 in raw milk from the North Khorasan Province, Iran, was 18.44±34.6 ng/L, while pasteurized and sterilized milk had a higher mean concentration of 42.8±21.54 ng/L. The lowest concentration was found in Bojnord City, Iran, at 9.30±8.91 ng/L, while the highest concentration was detected in Jajarm at 30.70±80.50 ng/L. The mean contamination of aflatoxin M1 was not statistically significant (P=0.42) in most cities of the province. Meanwhile, 6.34% of raw milk and 11.4% of pasteurized milk samples had higher levels of aflatoxin M1 than the maximum limit recommended by the Iran National Standard Organization (INSO) (100 ng/L). However, none of the milk samples exceeded the permissible limit set by the Veterinary Organization of the country (500 ng/L). Conclusion: The mean concentration of aflatoxin M1 in raw and pasteurized milk from the North Khorasan Province is lower than the European Union (EU) standard of 50 ng/L and the approved limit set by the country’s Veterinary Organization (500 ng/L). Therefore, it is not considered a significant threat to the health of adults.

Abstract The food industry is adapting to evolving consumer demands for “healthy” and premium quality food by reducing the adverse effects of food packaging through innovative advancements in active and intelligent packaging technologies. These smart innovations offer diverse and creative ways to enhance food product quality and safety while extending shelf life. Emerging techniques are also improving the passive aspects of food packaging systems, such as thermal stability, barrier effectiveness, and mechanical strength. Notably, the use of plants, biodegradable materials, and nanomaterials in sustainable food packaging helps mitigate its negative environmental impact. By integrating intelligent, environmentally friendly, and active packaging technologies, a multipurpose food packaging system can be developed that maintains the integrity of all its components, representing the pinnacle of technological advancement in food packaging. This article reviews the fundamentals of food packaging systems, recent advancements in various packaging techniques, commercialized patents, future research trends, and the challenges that need to be addressed in food packaging.

Background: Food poisoning could be caused by eating the sweets contaminated with microorganisms, which has always been considered as one of the major problems of people in developing countries, including Iran. The aim of this study is to investigate microbial variety and fungal load in the traditional sweets offered in confectionery stores in Yazd province in 2020. Methods: In this study, 170 samples of traditional sweets, referred by confectioneries in Yazd province, were randomly selected and tested for microorganisms contamination such as Enterobacteriaceae, Escherichia coli, Staphylococcus aureus, Salmonella, molds, and yeasts according to Iranian national standards. Results: The highest rate of microbial contamination in samples was related to "Pistachio Loz" (52.94%) and the lowest rate was related to "Hajibadam" (11.76%). The infection rates of Enterobacteriaceae, Escherichia coli, molds, and yeasts were 17.06%, 8.83%, 20.59%, 3.53%, respectively. No infection was observed with Staphylococcus aureus and Salmonella in the samples. Conclusions: Due to the high level of microbial contamination of traditional sweets, especially "Pistachio Loz" offered in Yazd, more control measures are needed in preparation and distribution of such sweets.

Background: Salmonella enterica serotype Typhi (Salmonella Typhi) causes severe and occasionally life-threatening disease, transmitted through contaminated food and water. Humans are the only reservoir, inadequate water, sanitation, and hygiene infrastructure increases risk of typhoid. High-quality data to assess spatial and temporal relationships in disease dynamics are scarce. Methods: We analyzed data from a prospective cohort conducted in an urban slum area of Dhaka City, Bangladesh. Passive surveillance at study centers identified typhoid cases by microbiological culture. Each incident case (index case) was matched to two randomly selected index controls, and we measured typhoid incidence in the population residing in a geographically defined region surrounding each case and control. Spatial clustering was evaluated by comparing the typhoid incidence in residents of geometric rings of increasing radii surrounding the index cases and controls over 28 days. Temporal clustering was evaluated by separately measuring incidence in the first and second 14-day periods following selection. Incidence rate ratios (IRRs) were calculated using Poisson regression models. Results: We evaluated 141 typhoid index cases. The overall typhoid incidence was 0.44 per 100,000 person-days (PDs) (95% CI: 0.40, 0.49). In the 28 days following selection, the highest typhoid incidence (1.2 per 100,000 PDs [95% CI: 0.8, 1.6]) was in the innermost cluster surrounding index cases. The IRR in this innermost cluster was 4.9 (95% CI: 2.4, 10.3) relative to the innermost control clusters. Neither typhoid incidence rates nor relative IRR between index case and control populations showed substantive differences in the first and second 14-day periods after selection. Conclusion: In the absence of routine immunization programs, geographic clustering of typhoid cases suggests a higher intensity of typhoid risk in the population immediately surrounding identified cases. Further studies are needed to understand spatial and temporal trends and to evaluate the effectiveness of targeted vaccination in disrupting typhoid transmission. Author summary: New generation typhoid conjugate vaccines have been introduced in mass national campaigns and routine immunization programs in several typhoid-endemic countries. We evaluated evidence for spatial and temporal clustering of typhoid fever in an urban slum area of Dhaka, Bangladesh, where typhoid is highly endemic. We assessed 141 typhoid index cases and observed the highest incidence of typhoid in the 14-day period following case selection in the population residing closest (cluster of radii 0-50m) to the index case. Clustering of incident cases suggests a higher intensity of transmission, and the populations at highest risk of contracting typhoid are those living in close proximity to an index case. The risk exists for a period of at least 28 days. Where strong surveillance exists, vaccination of localized geographic areas surrounding cases might be effective in controlling disease outbreaks in low- or no-coverage settings. This approach could offer a practical and efficient strategy for countries with limited data and resources to support mass typhoid vaccination. Additional evaluations of spatial and temporal trends of typhoid transmission are required in endemic and non-endemic settings, and studies designed to evaluate the effectiveness and impact of targeted vaccination are needed to refine prevention and control strategies. [ABSTRACT FROM AUTHOR]

Introduction: This article presents data on the ecological situation of agricultural territories where small cattle are raised, focusing on the exposure dose rate, radon concentration in residential and social buildings, and the density of alpha and beta particle flux. The study also examines the levels of heavy metals (Pb, Cd, As, Hg) and radionuclides Cs-137 and Sr-90 in raw meat from ecologically unfavorable areas, as well as the effects of heat treatment on reducing the content of these harmful substances. Methods: The research involved analyzing raw meat samples from the Abai district near the former Semipalatinsk Nuclear Test Site in Semey, Kazakhstan. The levels of heavy metals and radionuclides were measured before and after heat treatment to assess the effectiveness of cooking in reducing these contaminants. Radioecological measurements were also conducted in residential and social buildings to evaluate radon concentration and alpha and beta particle flux. Results and discussion: The results of the study showed a significant decrease in the levels of lead, cadmium, arsenic, Cs-137, and Sr-90 in meat after heat treatment. Prolonged cooking or quenching led to the most significant reductions in toxicity and radionuclides. These findings suggest that heat treatment can be an effective method for reducing the levels of harmful substances in meat from ecologically compromised areas. Further research is needed to explore other potential strategies for mitigating the impact of environmental contamination on food safety in agricultural regions. [ABSTRACT FROM AUTHOR]

Aflatoxin B1 (AFB1) contamination in food and feed is a global health and economic threat, necessitating the immediate development of effective strategies to mitigate its negative effects. This study focuses on the isolation and characterization of Enterococcus faecium HB2-2 (E. faecium HB2-2) as a potent AFB1-degrading microorganism, using morphological observation, biochemical profiling, and 16S rRNA sequence analysis. An incubation of E. faecium HB2-2 at 32 °C for 96 h in a pH 10 nutrient broth (NB) medium resulted in a remarkable degradation rate of 90.0% for AFB1. Furthermore, E. faecium HB2-2 demonstrated 82.9% AFB1 degradation rate in the peanut meal, reducing AFB1 levels from 105.1 to 17.9 μg/kg. The AFB1 degradation ability of E. faecium HB2-2 was found to be dependent on the fermentation supernatant. The products of AFB1 degradation by E. faecium HB2-2 were analyzed by liquid chromatography–mass spectrometry (LC-MS), and a possible degradation mechanism was proposed based on the identified degradation products. Additionally, cytotoxicity assays revealed a significant reduction in the toxicity of the degradation products compared to the parent AFB1. These findings highlight the potential of E. faecium HB2-2 as a safe and effective method for mitigating AFB1 contamination in food and feed. [ABSTRACT FROM AUTHOR]

Maintaining precise temperature control is vital for cold chain food transport, as temperature fluctuations can cause significant food safety and quality issues. During transport, ice that melts can promote the growth of microbes and their spread, resulting in microbial cross‐contamination. This study developed sustainable, non‐melting, self‐sanitizing "ice cubes" using food grade compositions including microporous cellulose sponges (MCS) and photosensitizers, aimed at enhancing temperature regulation and minimizing microbial contamination in the cold chain. Upon absorbing water, the MCS matched traditional ice in cooling efficiency and heat absorption and exhibit remarkable mechanical and thermal durability, withstanding multiple freeze‐thaw cycles and compressive stresses. The cationic MCS combined with erythrosine B demonstrated strong self‐sanitizing capabilities, effectively reducing microbial cross‐contamination in food models. Additionally, the release rates of photosensitizers from the MCS can be modulated by altering environmental ionic strength. This research offers viable solutions to address microbial cross‐contamination challenges in current cold chain systems. [ABSTRACT FROM AUTHOR]

Background and Aim: A total of 10% of the global population succumbs to amoebiasis yearly, equating to 50,000-100,000 recorded fatalities. It is closely associated with contaminated food and water supplies due to human feces. The disease's pathophysiology remains a subject of ongoing debate among experts. Some experts attribute the role of the host's conditions, parasite species and strain, and infection intensity in eliciting clinical symptoms. The aim of this study was to perform molecular identification of Entamoeba species isolated from humans and cattle. Materials and Methods: Stool samples from three hundred patients and one hundred cattle were collected from different regions, age groups, and sexes in Baghdad for microscopic examination. One hundred randomly chosen patient and cattle stool samples underwent microscopic examination and conventional polymerase chain reaction (PCR) targeting the 18S rRNA gene. Phylogenetic tree analyses were performed for Entamoeba species identification. Results: The infection rate in humans differed significantly (p < 0.05) between age groups and genders, totaling 38%. The infection rate in cattle, determined by conventional PCR, differed significantly (p < 0.05) between age groups and genders, amounting to 58%. Ten PCR products with positive results were sequenced and deposited in GenBank database. Sequence analysis detected that Eight sequences belong to E. histolytica (OM268853.1, OM268854.1, OM268855.1, OM268857.1, OM268858.1, OM268860.1, OM268861.1, and OM268862.1) and two sequences belong to E. dispar (OM268856.1 and OM268859.1) in humans, while 10 sequences (ON724165.1 to ON724174.1) belongs to E. histolytica in cattle. Conclusion: The increased susceptibility of cattle to E. histolytica suggests a considerable role in human infection and substantial public health risks. Further research should be conducted on the many virulence factors such as HM1:IMSS strain, cysteinprotease (Cp1), Gal/lectin, etc. of E. histolytica and E. dispar. [ABSTRACT FROM AUTHOR]

Aspergillus flavus is notorious for contaminating food with its secondary metabolite—highly carcinogenic aflatoxins. In this study, we found that exogenous nitric oxide (NO) donor could influence aflatoxin production in A. flavus. Flavohemoglobins (FHbs) are vital functional units in maintaining nitric oxide (NO) homeostasis and are crucial for normal cell function. To investigate whether endogenous NO changes affect aflatoxin biosynthesis, two FHbs, FHbA and FHbB, were identified in this study. FHbA was confirmed as the main protein to maintain NO homeostasis, as its absence led to a significant increase in intracellular NO levels and heightened sensitivity to SNP stress. Dramatically, FHbA deletion retarded aflatoxin production. In addition, FHbA played important roles in mycelial growth, conidial germination, and sclerotial development, and response to oxidative stress and high-temperature stress. Although FHbB did not significantly impact the cellular NO level, it was also involved in sclerotial development, aflatoxin synthesis, and stress response. Our findings provide a new perspective for studying the regulatory mechanism of the development and secondary mechanism in A. flavus. [ABSTRACT FROM AUTHOR]