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Climate change and warming will potentially profoundly affect forest growth and yield. However, climate change is not only a threat but also an opportunity for tree species to be more productive or colonize new territories where previously they were fragmented or absent. European beech (Fagus sylvatica L.) is a dominant and significant tree species in European forests with a pronounced ability to grow on a great variety of sites and to form mixtures with other tree species due to its shade tolerance. So far, only a few studies have analysed its growth and reaction to changing climatic conditions outside its natural gradient in the north. The aim of this study was therefore to clarify the main growth patterns and tree ring formation characteristics of beech (outside their natural distribution) in mixed stands with Scots pine (Pinus sylvestris L.) The analyses were carried out in two-layered stands in the western part of Lithuania with tree-ring data measured on stem disks collected at 1 m height that were available from shelter-cut thinning activities in a number of forest stands in the region. Our results show that higher temperatures during the summer months of July and August generally inhibited the radial growth of beech under given site conditions. In contrast, warmer late-autumn and cooler early-winter periods together with warmer springs positively affected the radial increments. Above-average precipitation during the vegetation period contributed to the beech producing additional amounts of radial increments. The increasing temperature trends of the summer months and decreasing monthly amounts of precipitation during the vegetation period in recent decades tend to have adverse effects on the ecological conditions for beech growth and vitality in Lithuania. Despite these results, the European beech successfully grows in the sample region and has produced 200 m3 ha−1 in the analysed stands. [ABSTRACT FROM AUTHOR]

Nanopore raw read accuracy has improved to over 99%, making it a potential tool for metabarcoding. For broad adoption, guidelines on quality filtering are needed to ensure reliable taxonomic unit recovery. This study aims to provide those guidelines for a fungal metabarcoding context and to apply them to a case study of ectomycorrhizae in the decaying bark of Fagus sylvatica. We introduce the eNano pipeline to test two standard metabarcoding approaches: (1) Reference-based mapping leveraging UNITE's species hypothesis system (SH approach); (2) Constructing 98% OTUs (OTU approach). Our results demonstrate that both approaches are effective with Nanopore data. When using a reference database, we recommend strict mapping criteria rather than Phred-based filtering. Leveraging the SH-system further enhances reproducibility and facilitates cross-study communication. For the 98% OTUs, filtering reads at ≥Q25 is recommended. Our case study reveals that the decay gradient is a primary determinant of community composition and that specific mycorrhizal fungi colonize decaying bark. Complementing our metabarcoding results with root tip morphotypification, we identify Laccaria amethystina and Tomentella sublilacina as key ectomycorrhizae of saplings on decaying logs. These findings demonstrate that Nanopore sequencing can provide valuable ecological insights and support its broader use in fungal metabarcoding as read quality continues to improve. [ABSTRACT FROM AUTHOR]

Riparian corridors often act as low-land climate refugia for temperate tree species in their southern distribution range. A plausible mechanism is the buffering of regional climate extremes by local physiographic and biotic factors. We tested this idea using a 3-year-long microclimate dataset collected along the Ciron river, a refugia for European beech (Fagus sylvatica) in southwestern France. Across the whole network, canopy gap fraction was the main predictor for spatial microclimatic variations, together with two other landscape features (elevation above the river and woodland fraction within a 300m radius). However, within the riparian forest only (canopy gap fraction < 25%, distance to the river < 150m), variations of up to -4°C and + 15% in summertime daily maximum air temperature and minimum relative humidity, respectively, were still found from the plateau to the cooler, moister river banks, only ~ 5-10m below. Elevation above the river was then identified as the main predictor, and explained the marked variations from the plateau to the banks much better than canopy gap fraction. The microclimate measured near the river is as cool but moister than the macroclimate encountered at 700-1000m asl further east in F. sylvatica's main distribution range. Indeed, at all locations, we found that air relative humidity was higher than expected from a temperature-only effect, suggesting that extra moisture is brought by the river. Our results explain well why beech trees in this climate refugium are restricted to the river gorges where microtopographic variations are the strongest and canopy gaps are rare. [ABSTRACT FROM AUTHOR]

Summary: Ectomycorrhizal (ECM) fungi distribute tree‐derived carbon (C) via belowground hyphal networks in forest ecosystems. Here, we asked the following: (1) Is C transferred belowground to a neighboring tree retained in fungal structures or transported within the recipient tree? (2) Is the overlap of ectomycorrhizal fungi in mycorrhizal networks related to the amount of belowground C transfer?We used potted sapling pairs of European beech (Fagus sylvatica) and North‐American Douglas‐fir (Pseudotsuga menziesii) for 13CO2 pulse‐labeling. We compared 13C transfer from beech (donor) to either beech or Douglas‐fir (recipient) and identified the ECM species. We measured the 13C enrichment in soil, plant tissues, and ECM fractions of fungal‐containing parts and plant transport tissues.In recipients, only fungal‐containing tissue of ectomycorrhizas was significantly enriched in 13C and not the plant tissue. Douglas‐fir recipients shared on average one ECM species with donors and had a lower 13C enrichment than beech recipients, which shared on average three species with donors.Our results support that recently assimilated C transferred belowground is shared among fungi colonizing tree roots but not among trees. In mixed forests with beech and Douglas‐fir, the links for C movement might be hampered due to low mycorrhizal overlap with consequences for soil C cycling. [ABSTRACT FROM AUTHOR]

In 2023, branches of European beech (Fagus sylvatica L.) were placed on 24 different study sites within beech stands in the Czech Republic to study the distribution of the beech bark beetle (Taphrorychus bicolor Her.). After infestation, the branches were placed in emergence traps to capture adults of the offspring generation. In total, 2167 adults of T. bicolor were captured across the 24 study sites. The average capture (mean ± SE) was 90.3 ± 24.4 adults per site. Statistical analyses, including GLMM and GLM, were employed to assess the influence of elevation and stand age on the abundance of T. bicolor. The results indicate that the distribution of T. bicolor is mainly influenced by the elevation and age of the beech stand. The number of captured adults decreased with elevation and increased with stand age. Climatic region, volumes of beech, and volumes of felled beech wood from principal felling and thinning felling had no significant effect on the number of T. bicolor adults captured. We found that, overall, 73.37% of Czech beech stands (at elevations 300–650 m a.s.l.) are possibly threatened (either highly or slightly) by the large occurrence of T. bicolor, because they are present at elevations where T. bicolor is widespread. No outbreaks of T. bicolor have yet been reported in the Czech Republic, but with the increasing amount of beech planted, combined with global climate change, the possibility of outbreaks in the future cannot be excluded. [ABSTRACT FROM AUTHOR]

Increasingly frequent and severe droughts pose significant threats to forest ecosystems, particularly affecting photosynthesis, a crucial physiological process for plant growth and biomass production. This study investigates the impact of phosphorus fertilization on the photosynthesis of common beech (Fagus sylvatica L.) and sessile oak (Quercus petraea (Matt.) Liebl.). In a common garden experiment, saplings originating from two provenances (wetter KA and drier SB provenances) were exposed to regular watering and drought in interaction with moderate and high phosphorus concentrations in the growing substrate. Results indicated that drought significantly reduced pre-dawn leaf water potential (ΨPD), net photosynthesis (Anet), stomatal conductance (gs) and photosynthetic performance index (PIabs) in both species. Phosphorus fertilization had a negative impact on Anet and PIabs, thus exacerbating the negative impact of drought on photosynthetic efficiency, potentially due to excessive phosphorus absorption by saplings. Provenance differences were notable, with the KA provenance showing better drought resilience. This research highlights the complexity of nutrient–drought interactions and underscores the need for cautious application of fertilization strategies in reforestation efforts under changing climatic conditions. [ABSTRACT FROM AUTHOR]

Marginal populations usually have low densities and are considered to be particularly vulnerable to environmental stochasticity. Using data collected in nest boxes, we analyzed the breeding performance of the edible dormouse (Glis glis), an obligate hibernating rodent and a seed predator in deciduous forests, in two populations at the distribution range's edge. Despite being only 20 km apart from each other, Montseny is a large patch of mixed deciduous forests (oaks and beech), whereas Montnegre would be the harshest habitat, that is, a small, isolated patch with only oaks. First, we studied the differences in climate and tree cover change in the two populations. Second, we analyzed the direct and indirect roles of local climate conditions and seed availability on breeding performance over 10 years in each population. Finally, we explored the influence of tree cover change on the occupancy dynamics in the two populations. Our results showed contrasting responses between populations: in Montseny, asynchronous seed production between oaks and beech precluded skip breeding, and breeding performance increased with seed availability. Furthermore, dormice in Montseny may use pollen production to anticipate the amount of beech nut resources and adjust their breeding effort. Boxes showed higher occupancy and colonization and fewer extinctions in Montseny than in Montnegre, where seed availability did not drive breeding performance. Results from Montnegre suggest that skip breeding was an adaptive response to a more pulsed, harsher environment. Here, females produced a similar number of pups than at Montseny. Long‐term studies dealing with population responses in marginal habitats can lead to a deeper understanding of the capacities of organisms to adapt to harsh environments. Although local adaptation is frequently documented across various taxa, studies at the distribution edge may shed light on our still limited comprehension of the underlying mechanisms responsible for its occurrence. [ABSTRACT FROM AUTHOR]

Due to an increasing risk of further damage to forests, forest managers are considering introducing an alternative direction for their future development - via the cultivation of mixed forests. At middle altitudes in the Czech Republic, an oak-beech-linden stand is the most natural type, and we tried to answer three main questions: (i) How the various thinning types affect dendrometric parameters and quality of the stand; (ii) How long thinning works on this stand until it loses its effect; (iii) How the stand develops spontaneously after abandonment. This experiment was conducted at the Training Forest Enterprise in the Czech Republic in Drahanská vrchovina (highlands in central Moravia). In 1988, four plots were established in a 49-year-old stand where, in three of the plots, different types of thinning (crown, low and heavy crown) were performed, leaving one (reference plot) to develop naturally. The height, the height of the crown base and diameter at breast height (DBH) were measured, and the shape and quality of the trunk and crown were estimated on each tree. Measurements were carried out in 1989, 1994, 1999, 2005, 2010, 2015, and 2020. In the first 10 years, the DBH and height of the crown base did not show any differences, and the linden at the heavy crown plot outgrew the linden trees at the other plots in height. After these 10 years, the thickest linden, the tallest beech and linden, and the greatest height of the crown base of beech and linden were all found at the heavy crown plot. The shape and quality of the trunks and crowns of beech, oak and linden were similar in all plots (including the reference plot) during the entire experiment. After thinning, the plots were left to grow spontaneously. The heavy crown thinning removed a greater number of thicker trees at the middle level, thus supporting the trees growing in the lower part of the middle level and in the below level (i.e. the beech and linden). These trees then grew more quickly compared to the others, but their quality decreased, as did that of the others. Therefore, a forest left to grow and develop spontaneously is practically unusable for commercial purposes.

Abstract Background European beech (Fagus sylvatica L.) trees produce seeds irregularly; therefore, it is necessary to store beech seeds for forestation. Despite the acquisition of desiccation tolerance during development, beech seeds are classified as intermediate because they lose viability during long-term storage faster than typical orthodox seeds. In this study, beech seeds stored for short (3 years) or long (20 years) periods under optimal conditions and displaying 92 and 30% germination capacity, respectively, were compared. Results Aged seeds displayed increased membrane damage, manifested as electrolyte leakage and lipid peroxidation levels. Analyses have been based on embryonic axes, which contained higher levels of reactive oxygen species (ROS) and higher levels of protein-bound methionine sulfoxide (MetO) in aged seeds. Using label-free quantitative proteomics, 3,949 proteins were identified, of which 2,442 were reliably quantified pointing to 24 more abundant proteins and 35 less abundant proteins in beech seeds under long-term storage conditions. Functional analyses based on gene ontology annotations revealed that nucleic acid binding activity (molecular function), ribosome organization or biogenesis and transmembrane transport (cellular processes), translational proteins (protein class) and membranous anatomical entities (cellular compartment) were affected in aged seeds. To verify whether MetO, the oxidative posttranslational modification of proteins that can be reversed via the action of methionine sulfoxide reductase (Msr) enzymes, is involved in the aging of beech seeds, we identified and quantified 226 MetO-containing proteins, among which 9 and 19 exhibited significantly up- and downregulated MetO levels, respectively, in beech seeds under long-term storage conditions. Several Msr isoforms were identified and recognized as MsrA1-like, MsrA4, MsrB5 and MsrB5-like in beech seeds. Only MsrA1-like displayed decreased abundance in aged seeds. Conclusions We demonstrated that the loss of membrane integrity reflected in the elevated abundance of membrane proteins had a higher impact on seed aging progress than the MetO/Msr system. Proteome analyses enabled us to propose protein Sec61 and glyceraldehyde-3-phosphate dehydrogenase as potential longevity modulators in beech seeds.

Abstract Background Carbon (C) sink and stock are among the most important ecosystem services provided by forests in climate change mitigation policies. In this context, old-growth forests constitute an essential reference point for the development of close-to-nature silviculture, including C management techniques. Despite their small extent in Europe, temperate old-growth forests are assumed to be among the most prominent in terms of biomass and C stored. However, monitoring and reporting of C stocks is still poorly understood. To better understand the C stock amount and distribution in temperate old-growth forests, we estimated the C stock of two old-growth stands in the Dinaric Alps applying different assessment methods, including direct and indirect approaches (e.g., field measurements and allometric equations vs. IPCC standard methods). This paper presents the quantification and the distribution of C across the five main forest C pools (i.e., aboveground, belowground, deadwood, litter and soil) in the study areas and the differences between the applied methods. Results We report a very prominent C stock in both study areas (507 Mg C ha− 1), concentrated in a few large trees (36% of C in 5% of trees). Moreover, we found significant differences in C stock estimation between direct and indirect methods. Indeed, the latter tended to underestimate or overestimate depending on the pool considered. Conclusions Comparison of our results with previous studies and data collected in European forests highlights the prominence of temperate forests, among which the Dinaric Alps old-growth forests are the largest. These findings provide an important benchmark for the development of future approaches to the management of the European temperate forests. However, further and deeper research on C stock and fluxes in old-growth stands is of prime importance to understand the potential and limits of the climate mitigation role of forests.

Summary: It is well established that solar irradiance greatly influences tree metabolism and growth through photosynthesis, but its effects acting through individual climate metrics have not yet been well quantified. Understanding these effects is crucial for assessing the impacts of climate change on forest ecosystems.To describe the effects of solar irradiance on tree growth, we installed 110 automatic dendrometers in two old‐growth mountain forest reserves in Central Europe, performed detailed terrestrial and aerial laser scanning to obtain precise tree profiles, and used these to simulate the sum of solar irradiance received by each tree on a daily basis. Generalized linear mixed‐effect models were applied to simulate the probability of growth and the growth intensity over seven growing seasons.Our results demonstrated various contrasting effects of solar irradiance on the growth of canopy trees. On the one hand, the highest daily growth rates corresponded with the highest solar irradiance potentials (i.e. the longest photoperiod). Intense solar irradiance significantly decreased tree growth, through an increase in the vapor pressure deficit. These effects were consistent for all species but had different magnitude.Tree growth is the most effective on long rainy/cloudy days with low solar irradiance. [ABSTRACT FROM AUTHOR]

The persistence of plant populations depends crucially on successful regeneration. Yet, little is known about the effects of consecutive winter and spring frost events on the regeneration stage of trees from different seed sources, although this will partly determine the success of climate warming‐driven poleward range shifts. In a common garden experiment with European beech (Fagus sylvatica) seedlings from winter 2015/2016 to autumn 2017, we studied how simulated successive spring and winter frost events affect leaf‐out dates, growth performance, and survival rates of 1‐ to 2‐year‐old seedlings from provenances differing in climate at origin. We further investigated the combined effects of successive frost events. The first spring frost after germination led to a mortality rate up to 75%, resulting in reduced seedling numbers but better frost tolerance of the survivors, as reflected in a weaker impact of the following winter frost event in the survivors compared to the non‐acclimated control. Final plant height was most strongly reduced by the spring frost in the second year. The winter frost event delayed leaf‐out by up to 40 days, leading to severe growth impairment in 2017. Our results indicate partly successful frost acclimation and/or the selection of frost‐hardier individuals, because the negative growth effects of consecutive frost events did not add up after exposure to more than one event. Both mechanisms may help to increase the frost tolerance of beech offspring. Nevertheless, mortality after the first spring frost was high, and frost exposure generally caused growth reductions. Thus, achieving higher frost tolerance may not be sufficient for beech seedlings to overcome frost‐induced reductions in competitive strength caused by winter frost damage and delayed leaf enfolding. [ABSTRACT FROM AUTHOR]

The hyperdiverse wood‐inhabiting fungi play a crucial role in the global carbon cycle, but often are threatened by deadwood removal, particularly in temperate forests dominated by European beech (Fagus sylvatica) and Oriental beech (Fagus orientalis). To study the impact of abiotic drivers, deadwood factors, forest management and biogeographical patterns in forests of both beech species on fungal composition and diversity, we collected 215 deadwood‐drilling samples in 18 forests from France to Armenia and identified fungi by meta‐barcoding. In our analyses, we distinguished the patterns driven by rare, common, and dominant species using Hill numbers. Despite a broad overlap in species, the fungal composition with focus on rare species was determined by Fagus species, deadwood type, deadwood diameter, precipitation, temperature, and management status in decreasing order. Shifting the focus on common and dominant species, only Fagus species, both climate variables and deadwood type remained. The richness of species within the deadwood objects increased significantly only with decay stage. Gamma diversity in European beech forests was higher than in Oriental beech forests. We revealed the highest gamma diversity for old‐growth forests of European beech when focusing on dominant species. Our results implicate that deadwood retention efforts, focusing on dominant fungi species, critical for the decay process, should be distributed across precipitation and temperature gradients and both Fagus species. Strategies focusing on rare species should additionally focus on different diameters and on the conservation of old‐growth forests. [ABSTRACT FROM AUTHOR]

Context: Evidence for declines in insect populations is growing with climate change being one suspected driver. Forests, however, are still underrepresented in the relevant research. Recent droughts (2018–2020) have severely affected forests in Central Europe and have been linked to declines in carabid abundance, biomass as well as changes in species traits at the local scale. Objective: We tested drought effects on forest carabids at regional scale. We additionally investigated whether variability in drought effects could be explained with the initial community composition and the local environmental context. Methods: We used generalized linear mixed models to compare data from 1999 to 2001 and 2020 to 2022 across eleven old beech forest sites of high conservation interest in North-East Germany and investigated changes in carabid abundance, biomass, Hill numbers and selected species traits. We then tested additional community-related and environmental predictors to explain spatial variability in changes in biomass. Results: We found significant declines in biomass of 65% and in abundance of 51%. There were no significant changes in Hill numbers. We found consistent evidence that declines affected especially larger and less mobile species. Declines and changes in species traits also occurred in strictly protected old-growth beech forests. Among environmental predictors, landscape composition explained local variability in biomass declines best with stronger decline at forest sites with less forest area in their vicinity. Conclusions: Our findings reveal large-scale declines in forest carabids in the context of recent droughts and highlight the exceptional role of landscape composition in this regard. Future insect conservation strategies need to incorporate the landscape context and potential exposure to extreme weather. [ABSTRACT FROM AUTHOR]

Summary: Extreme droughts can have long‐lasting effects on forest community dynamics and species interactions. Yet, our understanding of how drought legacy modulates ecological relationships is just unfolding. We tested the hypothesis that leaf chemistry and herbivory show long‐term responses to premature defoliation caused by an extreme drought event in European beech (Fagus sylvatica L.).For two consecutive years after the extreme European summer drought in 2018, we collected leaves from the upper and lower canopy of adjacently growing drought‐stressed and unstressed trees. Leaf chemistry was analyzed and leaf damage by different herbivore‐feeding guilds was quantified.We found that drought had lasting impacts on leaf nutrients and on specialized metabolomic profiles. However, drought did not affect the primary metabolome. Drought‐related phytochemical changes affected damage of leaf‐chewing herbivores whereas damage caused by other herbivore‐feeding guilds was largely unaffected. Drought legacy effects on phytochemistry and herbivory were often weaker than between‐year or between‐canopy strata variability.Our findings suggest that a single extreme drought event bears the potential to long‐lastingly affect tree–herbivore interactions. Drought legacy effects likely become more important in modulating tree–herbivore interactions since drought frequency and severity are projected to globally increase in the coming decades. [ABSTRACT FROM AUTHOR]

Background: European beech (Fagus sylvatica L.) trees produce seeds irregularly; therefore, it is necessary to store beech seeds for forestation. Despite the acquisition of desiccation tolerance during development, beech seeds are classified as intermediate because they lose viability during long-term storage faster than typical orthodox seeds. In this study, beech seeds stored for short (3 years) or long (20 years) periods under optimal conditions and displaying 92 and 30% germination capacity, respectively, were compared. Results: Aged seeds displayed increased membrane damage, manifested as electrolyte leakage and lipid peroxidation levels. Analyses have been based on embryonic axes, which contained higher levels of reactive oxygen species (ROS) and higher levels of protein-bound methionine sulfoxide (MetO) in aged seeds. Using label-free quantitative proteomics, 3,949 proteins were identified, of which 2,442 were reliably quantified pointing to 24 more abundant proteins and 35 less abundant proteins in beech seeds under long-term storage conditions. Functional analyses based on gene ontology annotations revealed that nucleic acid binding activity (molecular function), ribosome organization or biogenesis and transmembrane transport (cellular processes), translational proteins (protein class) and membranous anatomical entities (cellular compartment) were affected in aged seeds. To verify whether MetO, the oxidative posttranslational modification of proteins that can be reversed via the action of methionine sulfoxide reductase (Msr) enzymes, is involved in the aging of beech seeds, we identified and quantified 226 MetO-containing proteins, among which 9 and 19 exhibited significantly up- and downregulated MetO levels, respectively, in beech seeds under long-term storage conditions. Several Msr isoforms were identified and recognized as MsrA1-like, MsrA4, MsrB5 and MsrB5-like in beech seeds. Only MsrA1-like displayed decreased abundance in aged seeds. Conclusions: We demonstrated that the loss of membrane integrity reflected in the elevated abundance of membrane proteins had a higher impact on seed aging progress than the MetO/Msr system. Proteome analyses enabled us to propose protein Sec61 and glyceraldehyde-3-phosphate dehydrogenase as potential longevity modulators in beech seeds. [ABSTRACT FROM AUTHOR]

There are several highly damaging Phytophthora species pathogenic to forest trees, many of which have been spread beyond their native range by the international trade of live plants and infested materials. Such introductions can be reduced through the development of better tools capable of the early, rapid, and high-throughput detection of contaminated plants. This study utilized a volatilomics approach (solid-phase microextraction coupled to gas chromatography–mass spectrometry) to differentiate between several Phytophthora species in culture and discriminate between healthy and Phytophthora-inoculated European beech and pedunculate oak trees. We tentatively identified 14 compounds that could differentiate eight Phytophthora species from each other in vitro. All of the Phytophthora species examined, except Phytophthora cambivora, uniquely produced at least one compound not observed in the other species; however, most detected compounds were shared between multiple species. Phytophthora polonica had the most unique compounds and was the least similar of all the species examined. The inoculated seedlings had qualitatively different volatile profiles and could be distinguished from the healthy controls by the presence of isokaurene, anisole, and a mix of three unknown compounds. This study supports the notion that volatiles are suitable for screening plant material, detecting tree pathogens, and differentiating between healthy and diseased material. [ABSTRACT FROM AUTHOR]

Aim: Climate change challenges temperate forest trees by increasingly irregular precipitation and rising temperatures. Due to long generation cycles, trees cannot quickly adapt genetically. Hence, the persistence of tree populations in the face of ongoing climate change depends largely on phenotypic variation, that is the capability of a genotype to express variable phenotypes under different environmental conditions, known as plasticity. We aimed to quantify phenotypic variation of central Europe's naturally dominant forest tree across various intraspecific scales (individuals, mother trees (families), populations) to evaluate its potential to respond to changing climatic conditions. Location: Europe. Time Period: 2016–2019. Major Taxa Studied: European beech (Fagus sylvatica L.). Methods: We conducted a fully reciprocal transplantation experiment with more than 9000 beech seeds from seven populations across a Europe‐wide gradient. We compared morphological (Specific Leaf Area), phenological (leaf unfolding) and fitness‐related (growth, survival) traits across various biological scales: within single mother trees, within populations and across different populations under the contrasting climates of the translocation sites. Results: The experiment revealed significant phenotypic variation within the offspring of each mother tree, regardless of geographic origin. Initially, seedling height growth varied among mother trees and populations, likely due to maternal effects. However, the growth performance successively aligned after the first year. In summary, we observed a consistent growth response in different beech populations to diverse environments after initial maternal effects. Main Conclusions: The study strikingly demonstrates the importance of considering intraspecific variation. Given the surprisingly broad spectrum of phenotypes each mother tree holds within its juvenile offspring, we conclude that Fagus sylvatica might have the potential for medium‐term population persistence in face of climate change, provided that this pattern persists into later life stages. Hence, we also suggest further investigating the inclusion of passive adaptation and natural dynamics in the adaptive management of forests. [ABSTRACT FROM AUTHOR]

This study aimed to analyze the N storage capacity of litterfall and its impact on C mineralization in beech forests. The research was conducted at 15 sample plots under European beech stands located in different ecological conditions on the territory of Serbia. More than half (53%) of the sample plots are characterized by low and very low litterfall N content (

Phytophthora plurivora can affect a range of ecologically and silviculturally important tree species, including European beech (Fagus sylvatica), a common late successional tree species native to Europe. Here, we report on the high-quality genome of P. plurivora strain TJ71 (CBS 124093). We sequenced it using Oxford Nanopore MinION and PacBio Sequel II long-read sequencing with 80× coverage, chromatin conformation capture (Hi-C) sequencing with 400× coverage, and DNBSEQ 150-bp paired-end short reads sequencing with 200× coverage. This complex sequencing approach allowed for assembly of the genome at the chromosome level. Specifically, the P. plurivora genome resulted in 18 scaffolds of 47 Mbp total size with 95% completeness of the eukaryotic gene set as implemented in BUSCO. This is a considerable improvement relative to the previous NCBI reference genome of P. plurivora (NMPK00000000.1) with ∼41 Mbp organized in 1,898 scaffolds with 93.8% eukaryotic BUSCO completeness. This high-quality genome provides a valuable resource for further evolutionary, epidemiological, and population genomic studies. [Figure: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

Increasingly frequent and severe droughts pose significant threats to forest ecosystems, particularly affecting photosynthesis, a crucial physiological process for plant growth and biomass production. This study investigates the impact of phosphorus fertilization on the photosynthesis of common beech (Fagus sylvatica L.) and sessile oak (Quercus petraea (Matt.) Liebl.). In a common garden experiment, saplings originating from two provenances (wetter KA and drier SB provenances) were exposed to regular watering and drought in interaction with moderate and high phosphorus concentrations in the growing substrate. Results indicated that drought significantly reduced pre-dawn leaf water potential (ΨPD), net photosynthesis (Anet), stomatal conductance (gs) and photosynthetic performance index (PIabs) in both species. Phosphorus fertilization had a negative impact on Anet and PIabs, thus exacerbating the negative impact of drought on photosynthetic efficiency, potentially due to excessive phosphorus absorption by saplings. Provenance differences were notable, with the KA provenance showing better drought resilience. This research highlights the complexity of nutrient–drought interactions and underscores the need for cautious application of fertilization strategies in reforestation efforts under changing climatic conditions.

Досліджено сучасний санітарний стан бука лісового (Fagus sylvatica L.), ялиці білої (Abies alba Mill.) та ялини європейської (Picea abies (L.) Karsten) у Національному природному парку "Гуцульщина" та визначено першопричини їх ослаблення, втрати біотичної стійкості і продуктивності. З'ясовано, що сучасні екологічні виклики, зокрема такі як глобальні зміни клімату, антропогенний тиск, атаки шкідників та епіфітотійне поширення збудників інфекційних хвороб, є предикторами вразливості лісів, що призводить до порушення стратегій їхнього збереження і відновлення. Розраховано та проаналізовано середні значення показника санітарного стану за 2022-2023 рр. на підставі детального лісопатологічного обстеження 15 пробних площ, а також ідентифіковано видовий склад патологій інфекційного характеру у деревостанах дослідного регіону. Встановлено, що загальний санітарний стан лісів за участю бука лісового, ялиці білої та ялини європейської, що ростуть в умовах Національного природного парку "Гуцульщина", наразі потрібно вважати ослабленим. Середньозважений показник санітарного стану становить 2,50 з варіюванням від 1,39-3,55 (у 2022 р.) і 1,53-3,52 (у 2023 р.). Спостерігається повільне, проте стійке, збільшення площ розладнаних і загиблих деревостанів. Зафіксовано наявність дерев V і VІ категорій стану, осередки бактеріозу, мікозних хвороб і стовбурових шкідників. Під час обстежень відзначено типову симптоматику бактеріальної водянки ялиці (збудник – фітопатогенна бактерія Lelliottia nimipressuralis (Carter 1945)), раку ялиці (збудник – іржастий гриб Melampsorella cerastii (Pers.) J. Schröt. 1887), нектріозу бука (збудник – гриб-ксилотроф Neonectria ditissima (Tul., & C. Tul.) Samuels & Rossman 2006). Серед комплексу афілофороїдних макроміцетів найбільшу кількість видів зареєстровано на деревах бука європейського, найменшу – на ялиці білій. Найпоширенішою виявилася група ксилотрофних базидіоміцетів, серед яких зареєстровано збудники кореневих і стовбурових гнилей (гриби родів Heterobasidion Bref. 1888 та Armillaria (Fr.) Staude 1857, а також видів Phellinus hartigii (Allesch., & Schnabl) Pat., 1903, Fomitopsis pinicola (Sw.) P. Karst. 1881, Fomes fomentarius (L.) Fr. 1849 та ін). Відзначено, що врахування санітарного стану стає ключовим чинником під час розроблення наукових і практичних рішень для забезпечення стійкості та збереження корінних природних лісів Національного природного парку "Гуцульщина".

In the present work, single- and double-dowel joints following different geometric configurations are experimentally and numerically investigated to derive the splitting behaviour of beech wood (Fagus sylvatica L.), one of the most widespread hardwood species in Europe for structural purposes. The influence of the spacing between dowels, their distance to the supports, and the slenderness of the beams is analysed. The correlation of the experimental failure loads with those predicted numerically by cohesive zone finite element-based models using the fracture properties of the species is discussed. The experimental results are also compared with those obtained from the normative expression included in Eurocode 5 and two other design models reported in the literature. The splitting failure loads predicted by both the analytical and numerical models were found to be conservative, the latter being closer to the experimental values. [ABSTRACT FROM AUTHOR]

In this era of biodiversity loss and climate change, quantifying the impacts of natural disturbance on forest communities is imperative to improve biodiversity conservation efforts. Epiphytic and epixylic lichens are effective forest quality bioindicators, as they are generally long-lived organisms supported by continuity of specific forest structures and their associated microclimatic features. However, how lichen communities respond to the effects of fluctuating historical disturbances remains unclear. Using a dendrochronological approach, this study investigates how natural disturbance dynamics indirectly influence various lichen community metrics in some of Europe's best-preserved primary mixed-beech forests. Mixed modelling revealed that natural historical disturbance processes have decades-long effects on forest structural attributes, which had both congruent and divergent impacts on lichen community richness and composition. Total species richness indirectly benefited from both historical and recent higher-severity disturbances via increased standing dead tree basal area and canopy openness respectively - likely through the presence of both pioneer and late-successional species associated with these conditions. Red-listed species richness showed a dependence on habitat continuity (old trees), and increased with disturbance-related structures (standing dead trees) whilst simultaneously benefiting from periods without severe disturbance events (old trees and reduced deadwood volume). However, if the disturbance occurred over a century in the past, no substantial effect on forest structure was detected. Therefore, while disturbance-mediated forest structures can promote overall richness, threatened species appear vulnerable to more severe disturbance events -- a concern, as disturbances are predicted to intensify with climate change. Additionally, the high number of threatened species found reinforce the critical role of primary forest structural attributes for biodiversity maintenance. Hence, we recommend a landscape-scale conservation approach encompassing forest patches in different successional stages to support diverse lichen communities, and the consideration of long-term disturbance dynamics in forest conservation efforts, as they provide critical insights for safeguarding biodiversity in our changing world. [ABSTRACT FROM AUTHOR]

Deadwood plays a key role for biodiversity in forests. A significant number of beetles from this group are currently listed as endangered in the Red List. In addition to other management recommendations, there have been recent efforts to enrich stands with dead wood to promote biodiversity. An important parameter for forestry enterprises is the optimization of these interventions. The study investigated the abundance, species richness, gamma diversity, conservation value and biomass of saproxylic beetles in natural forests using window traps. A total of 89 traps were used for saproxylic beetle monitoring, of which 29 were placed on lying logs, 30 on snags and 30 as controls in forest stand space. A total of 35,011 beetles were recorded in 564 species (61 families). Notably, 20,515 of these belong to saproxylic beetles (59%) in 311 species (55%), with 62 classified as Red-List species (20%). In the group of 'all saproxylic beetles', the results indicate that α diversity and γ diversity (q ¼ 0) remain consistent across various deadwood types, while β diversity showed significant differences. Significant differences were found in the Red-List species group, where α diversity and γ diversity differed, with higher values observed in snags. Rarefaction based total species richness of site was estimated to be 391 species, including 74 Red-Listed species. Comparing the sample coverage of the studied stand categories showed that all saproxylic species exhibit a pronounced preference for inhabiting areas featuring lying logs. Conversely, Red-List species mainly inhabit snags, with β diversity being more similar to snags and forest stand space. Notably, both the conservation value (weighted average by conservation status) and beetle biomass are significantly highest in snags, whereas stand space shows the lowest values across all measured saproxylic beetle indices. Furthermore, the use of traps set on the poles in forest stand space resulted in an underestimation of the actual stand richness by 20%--25%. Our results support the conclusion that snags are indispensable features in beech forests, playing a key role in promoting high species diversity, especially among Red-List species, and supporting the biomass of saproxylic beetles. Consequently, it becomes crucial to incorporate a higher percentage of standing deadwood in managed forests or actively create equivalent environments by introducing high stumps. [ABSTRACT FROM AUTHOR]

The negative impact of drought on plant growth may be modified by the different availability of mineral nutrients and by their adaptation to different local habitat conditions. In this study, we examine the impact of drought, fertilization with phosphorus and provenance, as well as their interactions, on the growth and allometric growth relationships between the belowground and aboveground organs of common beech (Fagus sylvatica L.) and sessile oak (Quercus petraea (Matt.) Liebl.). The research was conducted on saplings originating from two mature mixed stands (dry and wet provenances) dominated by these species. In the common garden experiment, saplings were exposed to regular watering and drought in interaction with moderate and high phosphorus concentrations in the growing substrate (achieved by phosphorus fertilization). The obtained results indicate the negative impact of drought and phosphorus fertilization on the growth of both species. In common beech, a negative impact of phosphorus fertilization on the adaptive capacity to drought was demonstrated by unfavorable ratios between fine root mass and the mass of other organs. The sessile oak provenances under the impact of drought showed a different root collar diameter/stem height increment ratio, which indicates their different phenotypic plasticity as a consequence of adaptation to different frequencies of dry periods in their natural habitats. [ABSTRACT FROM AUTHOR]

Summary: Climate warming advances the onset of tree growth in spring, but above‐ and belowground phenology are not always synchronized. These differences in growth responses may result from differences in root and bud dormancy dynamics, but root dormancy is largely unexplored.We measured dormancy in roots and leaf buds of Fagus sylvatica and Populus nigra by quantifying the warming sum required to initiate above‐ and belowground growth in October, January and February. We furthermore carried out seven experiments, manipulating only the soil and not air temperature before or during tree leaf‐out to evaluate the potential of warmer roots to influence budburst timing using seedlings and adult trees of F. sylvatica and seedlings of Betula pendula.Root dormancy was virtually absent in comparison with the much deeper winter bud dormancy. Roots were able to start growing immediately as soils were warmed during the winter. Interestingly, higher soil temperature advanced budburst across all experiments, with soil temperature possibly accounting for c. 44% of the effect of air temperature in advancing aboveground spring phenology per growing degree hour.Therefore, differences in root and bud dormancy dynamics, together with their interaction, likely explain the nonsynchronized above‐ and belowground plant growth responses to climate warming. [ABSTRACT FROM AUTHOR]

The Ellenberg quotient (EQ) is a climate index defined as a ratio of the hottest month's temperature and the average annual precipitation sum. The quotient indirectly expresses the relationship between climate and vegetation, and its application is related to the ecological niche of Fagus sp. Although the quotient was curated on the grounds of field research primarily on German vegetation, the possibilities of its utilisation are not limited to the Central European region. The objective of this study is (i) to compare the EQ values calculated for the forest vegetation zones in the Czech Republic with the published data using the ecological niche of Fagus sylvatica; and (ii) to compare the new EQ-based vertical model with field empirical mapping. The study area is the Czech Republic, Central Europe. We used climate data from 1970-2000 and the data of the National Forest Inventory, 2nd cycle (2011-2015), representing an objective data design. Geospatial analytic methods, machine learning (boosting), and verification through statistical testing were performed. The results indicate higher EQ values between the two most substantial spatial frames - the Hercynicum and Carpaticum regions. By comparing empirical mapped units to their climatic potential (in the EQ), a match was found only within the Carpaticum region. The study presents a concretisation of the general climate index for a specific region, adds to the knowledge about the Fagus ecological niche in context with the Central European vegetation, and also points to the EQ's potential for evaluating the concept of vertical differentiation of forest communities, as well as a possible prediction tool for the vegetation migration in context with climate change.

Deadwood plays a key role for biodiversity in forests. A significant number of beetles from this group are currently listed as endangered in the Red List. In addition to other management recommendations, there have been recent efforts to enrich stands with dead wood to promote biodiversity. An important parameter for forestry enterprises is the optimization of these interventions. The study investigated the abundance, species richness, gamma diversity, conservation value and biomass of saproxylic beetles in natural forests using window traps. A total of 89 traps were used for saproxylic beetle monitoring, of which 29 were placed on lying logs, 30 on snags and 30 as controls in forest stand space. A total of 35,011 beetles were recorded in 564 species (61 families). Notably, 20,515 of these belong to saproxylic beetles (59%) in 311 species (55%), with 62 classified as Red-List species (20%). In the group of ‘all saproxylic beetles’, the results indicate that α diversity and γ diversity (q ​= ​0) remain consistent across various deadwood types, while β diversity showed significant differences. Significant differences were found in the Red-List species group, where α diversity and γ diversity differed, with higher values observed in snags. Rarefaction based total species richness of site was estimated to be 391 species, including 74 Red-Listed species. Comparing the sample coverage of the studied stand categories showed that all saproxylic species exhibit a pronounced preference for inhabiting areas featuring lying logs. Conversely, Red-List species mainly inhabit snags, with β diversity being more similar to snags and forest stand space. Notably, both the conservation value (weighted average by conservation status) and beetle biomass are significantly highest in snags, whereas stand space shows the lowest values across all measured saproxylic beetle indices. Furthermore, the use of traps set on the poles in forest stand space resulted in an underestimation of the actual stand richness by 20%–25%. Our results support the conclusion that snags are indispensable features in beech forests, playing a key role in promoting high species diversity, especially among Red-List species, and supporting the biomass of saproxylic beetles. Consequently, it becomes crucial to incorporate a higher percentage of standing deadwood in managed forests or actively create equivalent environments by introducing high stumps.

This article is a Commentary on Mas et al. (2024), 241: 1021–1034. [ABSTRACT FROM AUTHOR]

Species-rich mixed silver fir (Abies alba Mill.) forests dominated in the northern Apennines, but climate and, mainly, anthropogenic land use provoked a sharp silver fir decline approximately 5000 years bp. The conservation of the silver fir in this region was mainly due to the establishment of monastic orders that preserved and even planted silver fir for its spiritual and economic value. In 1993, the best silver fir stands were included in the Parco Nazionale delle Foreste Casentinesi (FCNP), Monte Falterona e Campigna, and have been submitted to low-intensive management or strict protection regardless of past land use and cultural history. In this study, we have (1) analyzed the current structure of three silver fir forests that have had different ownership histories and (2) compared the structure of the three Italian forests among them and with two old-growth forests from the Dinaric Alps as a reference of naturalness. The results show that the current structures of the three Italian forests are very different among them and are strictly related to past land use and, mainly, to monastic legacies. Even if the Italian forests have experienced decades of low-intensity management or strict protection, they are currently structurally very different from Dinaric old-growth forests. Developing an old-growth structure in these forests can be very slow and, in some ways, unpredictable. The results also highlight the importance of recognizing protected areas as cultural landscapes that host an important biocultural diversity. The current risk is that by applying almost exclusive biodiversity-centered management and setting difficult or impossible-to-achieve biodiversity goals, total diversity will decrease, and biocultural diversity, the greatest richness of most European parks, will be lost.

Rear‐edge tree populations are experiencing a combination of higher temperatures and more intense droughts that might push individuals beyond their tolerance limits. This trend towards rising atmospheric [CO2] is concurrent with an increase in intrinsic water use efficiency (iWUE), which theoretically enhances photosynthesis and decrease evapotranspiration rates, consequently improving tree resistance to drought. However, it remains unclear whether iWUE is favouring tree growth under current climate conditions, particularly when climate and iWUE legacy effects are simultaneously considered.We evaluated this question with an extensive sampling along Iberian rear‐edge (dry) populations comprising four mountain ranges and two distinct altitudes. We simultaneously examined the effects of climate and iWUE on secondary growth using annually resolved basal area increments (BAIs) for the period 1901–2017. We used linear mixed models including second‐order autocorrelation and 1‐year legacy effects of iWUE and summer drought.BAI and iWUE increased across the studied period. iWUE increase was driven by changes in atmospheric CO2 concentration and water availability during the growing season. Climate and iWUE exerted direct and lagged effects on beech growth. Water availability during growing season was the main driver of tree growth, combining direct and indirect effects through its impact on iWUE. Legacy effects of water availability and iWUE were more important than growing season conditions. The net effect of iWUE shifted when lagged effects were considered, resulting in a net negative impact on tree growth.Synthesis: Our results reveal that climate and iWUE legacy effects must be considered to assess the net iWUE effect on secondary growth. Considering lagged effects, the current increase in iWUE is constraining tree growth. Modelling efforts of tree growth response to climate warming should include climate and iWUE legacy effects to adequately assess terrestrial ecosystem carbon balance. [ABSTRACT FROM AUTHOR]

A change in tree species composition in Central Europe to increase the resilience of forests when coping with climate change effects is imminent. We evaluated the present and expected future role of the European beech, (Fagus sylvatica L.), with respect to the expansion of its habitat and its stem. We assessed the current relevance of beech from data of the Austrian Forest Inventory 2007/09, and analyzed forest policies promoting the establishment of beech forests. We simulated forest growth with the model CALDIS, linked with the timber-market model FOHOW2. We used a business-as-usual (BAU) forest management strategy under moderate (RCP 4.5 BAU) or extreme (RCP 8.5 BAU) climate change. We also simulated an extreme climate change scenario with a forced change in the tree species composition (RCP 8.5 Change Species). Beech occurs in Austrian forests over the elevational gradient of 250 to 1600 m a.s.l. In low elevation, it forms beech-dominated forests, often for the supply of the domestic fuelwood demand. In mountain regions, beech enriches the diversity of Norway spruce, (Picea abies (L.) H. Karst.)-dominated forests. In a BAU setting, the habitat of beech increases only slightly in both climate scenarios. The scenario 'RCP 8.5 Change Species' increases the habitat of beech in the next 60 years considerably. With progressing warming, other broadleaved tree species gain relevance. The vulnerability to drought and pathogens are limiting factors for Austrian beech forests. The future habitat depends on many factors such as the ability of forests to cope with climate change, the confidence to arising market opportunities of beech timber in the wake of technological progress, and financial and non-financial incentives for the implementation of adaptive forest management. [ABSTRACT FROM AUTHOR]

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Key message The datasets describe bacterial and fungal communities of European beech (Fagus sylvatica) leaves collected along a vertical gradient in a gallery forest throughout the growing season. They also describe communities in the surrounding environment of beech trees. Dataset access is at https://doi.org/10.7910/DVN/FFHAQU , and associated metadata are available at https://metadata-afs.nancy.inra.fr/geonetwork/srv/fre/catalog.search#/metadata/f17fe848-fc3e-4297-be11-9871b35a1be4 . Both can be used to uncover the dynamics and assembly processes of phyllosphere microbial communities in forest ecosystems.

Abstract Pulsed resources have prominent effects on community and ecosystem dynamics; however, there is little research on how resource pulses affect human–wildlife interactions. Tree masting is a common type of pulsed resource that represents a crucial food for many species and has important bottom‐up effects in food webs. In anthropogenic landscapes, years of food shortage after mast years can have negative outcomes for both people and wildlife, for instance when an increased use of anthropogenic foods by animals exacerbates human–wildlife conflicts. Here, we used novel remote sensing indicators of forest productivity and phenology, together with weather cues and ground measures of mast production, to assess whether years of masting and crop failures lead to changes in human–wildlife conflict occurrence. We used a unique 14‐year dataset including the production of European beech Fagus sylvatica seeds and brown bear Ursus arctos damage in the northeastern Carpathians as our model system. Linking these data in a panel regression framework, we found that temporal fluctuations in damage occurrence were sensitive to the year‐to‐year variation in beechnut production. Specifically, the number of damages during bear hyperphagia (i.e., September to December, when bears need to accumulate fat reserves prior to hibernation) was significantly higher in years with low beechnut production than in normal or mast years. Furthermore, we provide evidence that beech masting and failure can be predicted through a combination of remote‐sensing, weather, and field indicators of forest productivity and phenology. We demonstrate how pulsed resources, such as tree masting, can percolate through food webs to amplify human–wildlife conflict in human‐dominated landscapes. Given the recent range expansion of large carnivores and herbivores in many regions, including Europe, predicting years of natural food shortage can provide a pathway to proactive damage prevention, and thus to foster coexistence between wildlife and people.

Two provenance tests in North-Eastern Bulgaria have been studied, whose purpose is to test the response of beech provenances from Southern Germany to the drier and warmer climatic conditions in Bulgaria, in order to predict how they would perform in a warming and drying climate. The provenance tests were established in the spring of 2010 in the area of the Varbitza and Kipilovo Forest Services with 2-year-old seedlings in a 2 x 1 m scheme in 3 replications. Four provenances of common beech were studied - 2 German (Silberbach and Ebersdorf) and 2 Bulgarian (Petrohan and Berkovitza). On the 12th year after afforestation, an inventory and measurements of the height and root collar diameter were carried out. The results were processed statistically by applying one and two-factor analysis of variance, as well as Tukey’s test for multiple comparisons. A higher survival rate was found in the provenance test in Kipilovo. The Bulgarian provenances Petrohan and Berkovitza were characterized by better growth in height in both provenance tests, and in root collar diameter - provenance Petrohan. Provenance Ebersdorf could be recommended for use in drier and warmer places in Southern Germany.

The cerambycid beetle Rosalia alpinais associated with temperate, broadleaved (mainly beech) forests containing dead or decaying wood. This species is protected under the Habitats Directive of the European Union. Given its narrow ecological niche and limited dispersal abilities, habitat fragmentation is a conservation concern for populations of R. alpina. In order to maximise the effectiveness of habitat restoration, a scientifically sound procedure for patch selection is needed. In Gipuzkoa (N Spain), we used Light Detection and Ranging (LiDAR) images to search for 20x20-m cells matching the parameterisation of a predictive and local habitat model for R. alpinaat the tree level. The cells selected under quantitative criteria were clustered to identify potential habitat patches. Conefor Sensinode software was used to estimate the importance of each of those patches in terms of the connectivity of the population, based on dispersal distances of R. alpinaand the probabilities of dispersing events among patches. We identified 380 potential habitat patches, mostly in the south-eastern sector of the study area, which were classified into “core areas” and “connecting areas”. The performance of the model was tested in the field (61% of correct assignations), although the actual occurrence of R. alpinawithin the habitat patches should be assessed in the future. This model represents a step forward in guiding the cost-effective implementation of conservation activities, through a strict preservation of the current core habitat patches and an increase in the size of connecting patches.Therefore, we show that connectivity models combining remote sensing data and local habitat selection can be an aid in conservation planning and restoration actions, probably outperforming less efficient strategies, such as random or expert selection. RESUMEN: El escarabajo cerambícido Rosalia alpinaestá asociado a los bosques templados de frondosas (principalmente hayas) que albergan madera muerta o en descomposición. La especie está protegida bajo la Directiva de Hábitats de la Unión Europea. Dado su estrecho nicho ecológico y su limitada capacidad de dispersión, la fragmentación de su hábitat es un problema de conservación para las poblaciones de R. alpina. Para maximizar la efectividad de la restauración del hábitat, se necesita un procedimiento científicamente sólido para la selección de parches forestales sobre los que intervenir. En Gipuzkoa (norte de España) utilizamos imágenes LiDAR para buscar celdas de 20x20 m que se ajustaran a la parametrización de un modelo predictivo local de hábitat para R. alpinaa nivel de árbol. Las celdas seleccionadas bajo criterios cuantitativos fueron agrupadas para identificar parches de hábitat potencial. Se utilizó el software Conefor Sensinode para estimar la importancia de cada uno de estos parches en términos de conectividad para la población, con base en las distancias de dispersión de R. alpinay la probabilidad de eventos de dispersión entre parches. Se identificaron 380 parches de hábitat potencial, mayoritariamente en el sector sureste del área de estudio, que fueron clasificados como “áreas centrales” o “áreas de conexión”. La ejecutoria del modelo fue testada en campo (61 % de asignaciones correctas), si bien la presencia real de R. alpinaen los parches de hábitat debería ser confirmada en el futuro. Este modelo constituye un avance para guiar la implementación de actividades de conservación de manera eficiente, mediante la protección estricta de “áreas centrales” y el incremento del tamaño de las “áreas de conexión”. Por lo tanto, mostramos que los modelos de conectividad que combinan datos de detección remota y selección local del hábitat pueden asistir en la planificación de la conservación y en las acciones de restauración específicas, seguramente mejorando los resultados de estrategias menos eficientes, como la selección al azar o basada en criterio experto.

Summary: Roots of forest trees are colonized by a diverse spectrum of ectomycorrhizal (EM) fungal species differing in their nitrogen (N) acquisition abilities. Here, we hypothesized that root N gain is the result of EM fungal diversity or related to taxon‐specific traits for N uptake.To test our hypotheses, we traced 15N enrichment in fine roots, coarse roots and taxon‐specific ectomycorrhizas in temperate beech forests in two regions and three seasons, feeding 1 mM NH4NO3 labelled with either 15NH4+ or 15NO3−.We morphotyped > 45 000 vital root tips and identified 51 of 53 detected EM species by sequencing. EM root tips exhibited strong, fungal taxon‐specific variation in 15N enrichment with higher NH4+ than NO3− enrichment. The translocation of N into the upper parts of the root system increased with increasing EM fungal diversity. Across the growth season, influential EM species predicting root N gain were not identified, probably due to high temporal dynamics of the species composition of EM assemblages.Our results support that root N acquisition is related to EM fungal community‐level traits and highlight the importance of EM diversity for tree N nutrition. [ABSTRACT FROM AUTHOR]

Transpiration and sap flow are physiologically interconnected processes that regulate nutrient and water uptake, controlling major aspects of tree life. They hold special relevance during drought, where wrecked sap flow can undermine overall tree growth and development. The present study encompasses five-year (2012–2015 and 2017) sap flow datasets on European beech (Fagus sylvatica). Four different techniques were used for sap flow modeling, namely, a linear model (LM), random forest (RF), extreme gradient boosting machine (XGBM), and neural networks (NN). We used six variants (Variants 1–6) differing in the captured conditions and the dataset size. The 'prediction power' was the ratio of the predicted and observed sap flow. We found the LM had the maximum prediction power for the overall sap flow in beech trees with 1 h shift of global radiation. In the reaming variants, the LM provided comparable prediction power to RF and XGBM. At the same time, NN exhibited relatively poor prediction power over other machine learning models. The study supports an easier-to-apply and computationally simpler approach (LM) to assess sap flow over more sophisticated machine learning approaches (RF, XGBM, and NN). [ABSTRACT FROM AUTHOR]

In forest communities, light competition is a key process for community assembly. Species' differences in seedling and sapling tolerance to shade cast by overstory trees is thought to determine species composition at late‐successional stages. Most forests are distant from these late‐successional equilibria, impeding a formal evaluation of their potential species composition. To extrapolate competitive equilibria from short‐term data, we therefore introduce the JAB model, a parsimonious dynamic model with interacting size‐structured populations, which focuses on sapling demography including the tolerance to overstory competition. We apply the JAB model to a two‐"species" system from temperate European forests, that is, the shade‐tolerant species Fagus sylvatica L. and the group of all other competing species. Using Bayesian calibration with prior information from external Slovakian national forest inventory (NFI) data, we fit the JAB model to short time series from the German NFI. We use the posterior estimates of demographic rates to extrapolate that F. sylvatica will be the predominant species in 94% of the competitive equilibria, despite only predominating in 24% of the initial states. We further simulate counterfactual equilibria with parameters switched between species to assess the role of different demographic processes for competitive equilibria. These simulations confirm the hypothesis that the higher shade tolerance of F. sylvatica saplings is key for its long‐term predominance. Our results highlight the importance of demographic differences in early life stages for tree species assembly in forest communities. [ABSTRACT FROM AUTHOR]

The quality of container-grown seedlings is influenced by the air and water properties of the substrate. These properties are closely tied to the amount and frequency of water supplied through sprinkler systems in nurseries, as well as the density of the substrate in the container cells. Throughout the entire growing season, this study examined how various parameters of Scots pine, Norway spruce, European beech, and pedunculate oak seedlings cultivated in HIKO V120SS and V265 containers were affected by two factors. Firstly, the study analyzed the impact of increased substrate density when filling the containers. Secondly, it explored the precise dosing of water applied by the sprinkler system, which was determined based on substrate sensors and meteorological conditions surrounding the seedlings. The results revealed that increased substrate compaction led to a long-term reduction in air capacity and an increase in water capacity within pine, spruce, and beech containers. However, oak seedlings were not affected by the increased substrate density. Additionally, the higher density of the compacted substrate positively influenced the growth parameters of pine seedlings but did not affect the other species. As a result, the current substrate compaction level used in the nursery where the measurements were taken appears to be optimal for spruce, beech, and oak seedlings. Furthermore, precise control over the amount of water applied during irrigation allowed for a reduction in water consumption by about 8%. This control also resulted in improved seedling sturdiness quotient and a more developed root system in the case of pine seedlings. However, no significant differences were observed for the other species. [ABSTRACT FROM AUTHOR]

Earth's forests are increasingly exposed to climate risks through climate change-related drought and heat waves. Here, we review the climate vulnerability of European beech (Fagus sylvatica), the most abundant tree species of Central Europe's temperate forests, and its adaptation potential to a hotter and drier climate in northern Germany, close to the center of the species' distribution range. About two-thirds of the studied beech forests show persistent negative growth trends of their dominant trees since the onset of rapid warming in the early 1980s, driven primarily by long-term deterioration of the climatic water balance (CWB) in summer and, locally, long-term decrease in June precipitation. Lower water storage capacity of the soil increases the climate sensitivity of growth. Even though beech populations of drier sub-regions reveal some acclimation to drought (reduced stomatal conductance, turgor loss point reduction, higher C allocation to roots), they are more climate-vulnerable than populations at moister sites, visible in larger growth decreases and greater CWB sensitivity of growth. Even though beech mortality is still lower in the study region than in other parts of Central Europe, our results identify beech forest regions with <350 mm growing-season precipitation as vulnerable to recent climate conditions, which refers to half of the study region. With further climate warming and aridification, most of northern Germany likely will become unfavorable for beech growth within the next tree generation, which suggests choosing in many production forests alternative, more drought- and heat-resistant timber species. [ABSTRACT FROM AUTHOR]

The Ellenberg quotient (EQ) is a climate index defined as a ratio of the hottest month's temperature and the average annual precipitation sum. The quotient indirectly expresses the relationship between climate and vegetation, and its application is related to the ecological niche of Fagus sp. Although the quotient was curated on the grounds of field research primarily on German vegetation, the possibilities of its utilisation are not limited to the Central European region. The objective of this study is (i) to compare the EQ values calculated for the forest vegetation zones in the Czech Republic with the published data using the ecological niche of Fagus sylvatica; and (ii) to compare the new EQ-based vertical model with field empirical mapping. The study area is the Czech Republic, Central Europe. We used climate data from 1970–2000 and the data of the National Forest Inventory, 2nd cycle (2011–2015), representing an objective data design. Geospatial analytic methods, machine learning (boosting), and verification through statistical testing were performed. The results indicate higher EQ values between the two most substantial spatial frames – the Hercynicum and Carpaticum regions. By comparing empirical mapped units to their climatic potential (in the EQ), a match was found only within the Carpaticum region. The study presents a concretisation of the general climate index for a specific region, adds to the knowledge about the Fagus ecological niche in context with the Central European vegetation, and also points to the EQ's potential for evaluating the concept of vertical differentiation of forest communi)ties, as well as a possible prediction tool for the vegetation migration in context with climate change. [ABSTRACT FROM AUTHOR]

Abstract Environmental DNA consists of species‐specific intracellular and extracellular fractions, whose content and information may not be similar in all environments. In forest soil, in particular, the biogeochemical fate of DNA originated by plant litter input has been extensively reviewed, but species‐specific persistence and distribution still await to be quantified. In the present work, based on the purification of extracellular and intracellular DNA fractions from forest soil samples representing 3 soil horizons at 36 randomized locations differing for stand composition (either beech‐ or spruce‐dominated, and mixed), followed by exDNA metabarcoding with the rbcL marker, we provide a clear picture of species‐specific plant DNA distribution, and explore plant community composition and diversity along the explored gradient and the soil profile. We did not find significant differences in intra‐ vs. extracellular total DNA distribution, with a progressive depletion with soil depth positively associated with soil organic C and N content and negatively associated with soil pH and mineral content. Species‐specific DNA distribution was horizontally dependent on beech and spruce basal area aboveground, while extracellular DNA showed peculiar species‐specific vertical patterns. Proportion of Fagus sylvatica DNA increased with depth in beech stand soil, and Picea abies DNA decreased in spruce stand soil, respectively, possibly linked to species‐specific differences in leaf litter decomposition dynamics and root litter contributions. Finally, our approach by metabarcoding provided a faithful, although incomplete, picture of the local plant diversity, suggesting that such technique could positively integrate traditional biodiversity inventory studies based on expert field assessments.

Key message: The datasets describe bacterial and fungal communities of European beech (Fagus sylvatica) leaves collected along a vertical gradient in a gallery forest throughout the growing season. They also describe communities in the surrounding environment of beech trees. Dataset access is at https://doi.org/10.7910/DVN/FFHAQU, and associated metadata are available at https://metadata-afs.nancy.inra.fr/geonetwork/srv/fre/catalog.search#/metadata/f17fe848-fc3e-4297-be11-9871b35a1be4. Both can be used to uncover the dynamics and assembly processes of phyllosphere microbial communities in forest ecosystems. [ABSTRACT FROM AUTHOR]

Vegetation landscape dynamics are derived from the relationships established between anthropic activities and climate conditions over time. Paleoenvironmental research in the Pyrenees range (north-eastern Iberian Peninsula) has revealed what these dynamics were like in some regions during the Holocene. However, some fields of biogeography still present questions that need to be addressed, such as the patterns of Fagus sylvatica and Abies alba and the importance of the fire regime during the Meghalayan (late Holocene). We present a multiproxy study performed in a sedimentary record from the Clots de Rialba peat bog, located at 2093 m a. s. l. (Lleida, southern slope of the Pyrenees mountain range), that covers the last c. 3500 years. Analyses were performed on the organic matter content, pollen, non-pollen palynomorphs, and sedimentary charcoals larger than 150 μm. The palynological spectra revealed a maximum extension of Abies alba at about 3500 cal yr BP in the Bronze Age, while Fagus sylvatica showed its maximum extent between 3300 and 2800 cal yr BP. A dominance of Pinus was detected throughout the studied period. Other taxa such as cereals, herbaceous plants, aquatic plants, and coprophilous fungi have also been discussed to identify anthropic pressure and climate pulses. In addition, the study of sedimentary charcoals reveals the main forest fire episodes and their recurrences, some of them linked to anthropogenic activities and/or climate variations. These anthropogenic activities would include the use of opening and maintaining forest fires in deforestation in order to obtain pastures and spaces dedicated to cereal agriculture and the appearance of some taxa linked to or introduced by human communities. [ABSTRACT FROM AUTHOR]

Pulsed resources have prominent effects on community and ecosystem dynamics; however, there is little research on how resource pulses affect human–wildlife interactions. Tree masting is a common type of pulsed resource that represents a crucial food for many species and has important bottom‐up effects in food webs. In anthropogenic landscapes, years of food shortage after mast years can have negative outcomes for both people and wildlife, for instance when an increased use of anthropogenic foods by animals exacerbates human–wildlife conflicts. Here, we used novel remote sensing indicators of forest productivity and phenology, together with weather cues and ground measures of mast production, to assess whether years of masting and crop failures lead to changes in human–wildlife conflict occurrence. We used a unique 14‐year dataset including the production of European beech Fagus sylvatica seeds and brown bear Ursus arctos damage in the northeastern Carpathians as our model system. Linking these data in a panel regression framework, we found that temporal fluctuations in damage occurrence were sensitive to the year‐to‐year variation in beechnut production. Specifically, the number of damages during bear hyperphagia (i.e., September to December, when bears need to accumulate fat reserves prior to hibernation) was significantly higher in years with low beechnut production than in normal or mast years. Furthermore, we provide evidence that beech masting and failure can be predicted through a combination of remote‐sensing, weather, and field indicators of forest productivity and phenology. We demonstrate how pulsed resources, such as tree masting, can percolate through food webs to amplify human–wildlife conflict in human‐dominated landscapes. Given the recent range expansion of large carnivores and herbivores in many regions, including Europe, predicting years of natural food shortage can provide a pathway to proactive damage prevention, and thus to foster coexistence between wildlife and people. [ABSTRACT FROM AUTHOR]

European beech (Fagus sylvatica L.) is an important tree species throughout Europe but shifts in its suitable habitats are expected in the future due to climate change. Finding provenances that are still economically viable and ecologically resilient is an ongoing field of research. We modelled the dominant tree heights of European beech as a trait reflecting growth performance dependent on provenance, climate and soil conditions. We derived dominant tree heights from national forest inventory (NFI) data from six European countries spanning over large ecological gradients. We performed function-on-scalar regression using hierarchical generalized additive models (HGAM) to model both the global effects shared among all provenances and the effects specific to a particular provenance. By comparing predictions for a reference period of 1981–2010 and 2071–2100 in a RCP 8.5 scenario, we showed that changes in growth performance can be expected in the future. Dominant tree heights decreased in Southern and Central Europe but increased in Northern Europe by more than 10 m. Changes in growth performance were always accompanied by a change in beech provenances, assuming assisted migration without dispersal limitations. Our results support the concept of assisted migration for the building of resilient future forests and emphasize the use of genetic data for future growth predictions. [ABSTRACT FROM AUTHOR]