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International audience; The main objective of this research was to define a structured and functionalized support for future biomedical applications (model of “low-density bioarray”). The experiments were carried out by using stereolithography process with a special SU-8 photoresist and the reproducibility of the method was studied by analyzing the surface profile of the support. Finally, a matrix of regular controlled sized wells was fabricated. Chemical reactions leading to covalent grafting were run to demonstrate that the inner surface of the wells remains still reactive after polymerization. The grafting of fluorophores with carboxylic functions activated by N-hydroxysuccinimide was studied as function of time, in order to determine the best reactions, conditions. Then, the grafting of two distinct fluorescent probes was led simultaneously inside the wells, showing the possibility of spatial localization of diverse reactions on the same support. The covalent and localized bindings were confirmed by fluorescence spectroscopy and microscopy analyses.

International audience; The photocatalytic decolorization of industrial textile dyes has been studied. The treatment was carried out on a solar reactor consisting in a flat active plane, tilted so as to face the sun and to allow the trickling of the water to be treated. Alternatively the reactor could be irradiated by an artificial source. After checking the system using salicylic acid, a conventional model molecule, the photocatalytic decolorization of Orange II, Yellow Drimarene, and Black Drimarene dyes was investigated. Artificial and solar irradiation gave comparable results although the heating by the sun reduced the amount of adsorption. The kinetics agrees with the Langmuir-Hinshelwood model and a discrepancy between adsorption constants deduced from the kinetic and adsorption experiments was interpreted by considering various types of adsorption sites. Orange II andDrimarene dyes decolorization kinetics are opposite limiting cases of the above model, as being of order 0 and 1 with respect to the dye, respectively.