1. Chemical Secretory Pathway Modulation in Plant Protoplasts
- Author
-
Michele Bellucci, Francesca De Marchis, and Andrea Pompa
- Subjects
0106 biological sciences ,0301 basic medicine ,medicine.diagnostic_test ,Immunoprecipitation ,fungi ,food and beverages ,Tunicamycin ,biochemical phenomena, metabolism, and nutrition ,Protoplast ,Golgi apparatus ,Brefeldin A ,Immunofluorescence ,01 natural sciences ,Transport protein ,Cell biology ,03 medical and health sciences ,chemistry.chemical_compound ,symbols.namesake ,030104 developmental biology ,chemistry ,medicine ,symbols ,BFA ,Endo-H ,Golgi-mediated protein traffic ,Pulse-chase ,Tobacco ,Secretory pathway ,010606 plant biology & botany - Abstract
The classical Golgi pathway is not the only mechanism for vacuolar protein transport in plants because alternative transport mechanisms have been described. The existence of these alternative pathways can be demonstrated using several chemicals and here we describe the use of brefeldin A (BFA), endo-β-N-acetylglucosaminidase H (Endo-H), and tunicamycin, on isolated tobacco leaf protoplasts. Two main methods are illustrated in this chapter, protoplast pulse-chase followed by protein immunoprecipitation, and protoplast immunofluorescence.
- Published
- 2016