5,199 results on '"Electrophoresis"'
Search Results
2. Importance of the electrophoresis and pulse energy for siRNA-mediated gene silencing by electroporation in differentiated primary human myotubes.
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Pavlin, Mojca, Škorja Milić, Nives, Kandušer, Maša, and Pirkmajer, Sergej
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ELECTROPORATION , *GENETIC vectors , *ELECTROPHORESIS , *GENE silencing , *SMALL molecules , *MEMBRANE permeability (Biology) , *CELL survival , *GENE therapy - Abstract
Background: Electrotransfection is based on application of high-voltage pulses that transiently increase membrane permeability, which enables delivery of DNA and RNA in vitro and in vivo. Its advantage in applications such as gene therapy and vaccination is that it does not use viral vectors. Skeletal muscles are among the most commonly used target tissues. While siRNA delivery into undifferentiated myoblasts is very efficient, electrotransfection of siRNA into differentiated myotubes presents a challenge. Our aim was to develop efficient protocol for electroporation-based siRNA delivery in cultured primary human myotubes and to identify crucial mechanisms and parameters that would enable faster optimization of electrotransfection in various cell lines. Results: We established optimal electroporation parameters for efficient siRNA delivery in cultured myotubes and achieved efficient knock-down of HIF-1α while preserving cells viability. The results show that electropermeabilization is a crucial step for siRNA electrotransfection in myotubes. Decrease in viability was observed for higher electric energy of the pulses, conversely lower pulse energy enabled higher electrotransfection silencing yield. Experimental data together with the theoretical analysis demonstrate that siRNA electrotransfer is a complex process where electropermeabilization, electrophoresis, siRNA translocation, and viability are all functions of pulsing parameters. However, despite this complexity, we demonstrated that pulse parameters for efficient delivery of small molecule such as PI, can be used as a starting point for optimization of electroporation parameters for siRNA delivery into cells in vitro if viability is preserved. Conclusions: The optimized experimental protocol provides the basis for application of electrotransfer for silencing of various target genes in cultured human myotubes and more broadly for electrotransfection of various primary cell and cell lines. Together with the theoretical analysis our data offer new insights into mechanisms that underlie electroporation-based delivery of short RNA molecules, which can aid to faster optimisation of the pulse parameters in vitro and in vivo. [ABSTRACT FROM AUTHOR]
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- 2024
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3. Starch-Based Polysaccharide Systems with Bioactive Substances: Physicochemical and Wettability Characteristics.
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Wiącek, Agnieszka Ewa and Furmaniuk, Anna
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CONTACT angle , *POLYSACCHARIDES , *WETTING , *ZETA potential , *BIOLOGICAL interfaces , *LIGHT scattering , *DIELECTROPHORESIS , *HYSTERESIS - Abstract
Polysaccharide-based systems have very good emulsifying and stabilizing properties, and starch plays a leading role. Their modifications should add new quality features to the product to such an extent that preserves the structure-forming properties of native starch. The aim of this manuscript was to examine the physicochemical characteristics of the combinations of starch with phospholipids or lysozymes and determine the effect of starch modification (surface hydrophobization or biological additives) and preparation temperature (before and after gelatinization). Changes in electrokinetic potential (zeta), effective diameter, and size distribution as a function of time were analyzed using the dynamic light scattering and microelectrophoresis techniques. The wettability of starch-coated glass plates before and after modification was checked by the advancing and receding contact angle measurements, as well as the angle hysteresis, using the settle drop method as a complement to profilometry and FTIR. It can be generalized that starch dispersions are more stable than analogous n-alkane/starch emulsions at room and physiological temperatures. On the other hand, the contact angle hysteresis values usually decrease with temperature increase, pointing to a more homogeneous surface, and the hydrophobization effect decreases vs. the thickness of the substrate. Surface hydrophobization of starch carried out using an n-alkane film does not change its bulk properties and leads to improvement of its mechanical and functional properties. The obtained specific starch-based hybrid systems, characterized in detail by switchable wettability, give the possibility to determine the energetic state of the starch surface and understand the strength and specificity of interactions with substances of different polarities in biological processes and their applicability for multidirectional use. [ABSTRACT FROM AUTHOR]
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- 2024
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4. Synthesis, structural, molecular docking, and in vitro biological activities of Cu-doped ZnO nanomaterials.
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El-Sayed, Ahmed F., Aboulthana, Wael M., Sherief, Marwa A., El-Bassyouni, Gehan T., and Mousa, Sahar M.
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MOLECULAR docking , *ZINC oxide films , *ZINC oxide , *ESCHERICHIA coli , *PENICILLIN-binding proteins , *IONIC strength - Abstract
Copper-doped ZnO nanoparticles with the formula Zn1−x(Cu)O, where x = 0.0, 0.03, 0.05, and 0.07 were produced using the co-precipitation process. Physical, chemical, and structural properties were properly examined. Powdered X-ray diffraction (P-XRD) patterns revealed the formation of hexagonal wurtzite crystal structure in all samples, through atomic substitutional incorporation in the Cu-doped ZnO lattice. The presence of Cu ions and their dissolution in the host ZnO crystal structure was supported by FT-IR spectra. HR-TEM images were used to assess the average size, morphology, and shape regularity of the synthesized samples. The form and homogeneity of the ZnO changed when Cu ions were substituted, as evidenced by FE-SEM/EDX analysis. The presence of copper signals in the Cu-doped samples indicates that the doping was successful. The decrease in zeta potential with an increased copper doping percentage designates that the nanoparticles (NPs) are more stable, which could be attributed to an increase in the ionic strength of the aqueous solution. The synthesized NPs were evaluated for their substantial in vitro antioxidant properties. In addition, the antimicrobial efficacy of the materials was tested against pathogenic microorganisms. Regarding the anti-diabetic activity, the 7Cu ZnO sample showed the highest inhibitory effect on the α-amylase enzyme. No variations were observed in the activities of the acetylcholinesterase enzyme (AChE) and proteinase enzymes with ZnO and samples doped with different concentrations of Cu. Therefore, further studies are recommended to reveal the in-vitro anti-diabetic activity of the studied doped samples. Finally, molecular docking provided valuable insights into the potential binding interactions of Cu-doped ZnO with α-amylase, FabH of E. coli, and Penicillin-binding proteins of S. aureus. These outcomes suggest that the prepared materials may have an inhibitory effect on enzymes and hold promise in the battle against microbial infections and diabetes. [ABSTRACT FROM AUTHOR]
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- 2024
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5. Familial chylomicronemia syndrome: case reports of siblings with deletions of the GPIHBP1 gene.
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Kim, Ka Young, Heo, You Joung, Ko, Jung Min, Lee, Young Ah, Shin, Choong Ho, Ki, Chang Seok, and Lee, Yun Jeong
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HDL cholesterol , *HYPERCHOLESTEREMIA , *HYPERLIPOPROTEINEMIA , *AUTOANTIBODIES , *POLYMERASE chain reaction , *GENE expression , *PANCREATITIS , *LIPASES , *TRIGLYCERIDES , *GENETIC mutation , *ELECTROPHORESIS , *GENETIC testing , *SEQUENCE analysis - Abstract
Background: Familial chylomicronemia syndrome (FCS) is a rare monogenic form of severe hypertriglyceridemia, caused by mutations in genes involved in triglyceride metabolism. Herein, we report the case of a Korean family with familial chylomicronemia syndrome caused by compound heterozygous deletions of glycosylphosphatidylinositol-anchored high-density lipoprotein-binding protein 1 (GPIHBP1). Case presentation: A 4-year-old boy was referred for the evaluation of severe hypertriglyceridemia (3734 mg/dL) that was incidentally detected 4 months prior. His elder brother also demonstrated an elevated triglyceride level of 2133 mg/dL at the age of 9. Lipoprotein electrophoresis revealed the presence of chylomicrons, an increase in the proportion of pre-beta lipoproteins, and low serum lipoprotein lipase levels. The patient's parents and first elder brother had stable lipid profiles. For suspected FCS, genetic testing was performed using the next-generation sequencing-based analysis of 31 lipid metabolism-associated genes, which revealed no pathogenic variants. However, copy number variant screening using sequencing depth information suggested large heterozygous deletion encompassing all the coding exons of GPIHBP1. A real-time quantitative polymerase chain reaction was performed to validate the deletion site. The results showed that the siblings had two heterozygous copy number variants consisting of the whole gene and an exon 4 deletion, each inherited from their parents. During the follow-up period of 17 months, the patient did not develop pancreatitis, following dietary intervention. Conclusion: These siblings' case of familial chylomicronemia syndrome caused by rare GPIHBP1 deletions highlight the implementation of copy number variants—beyond next-generation sequencing—as an important consideration in diagnosis. Accurate genetic diagnosis is necessary to establish the etiology of severe hypertriglyceridemia, which increases the risk of pancreatitis. [ABSTRACT FROM AUTHOR]
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- 2024
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6. Metformin Enhances the Sensitivity of Glioblastoma Cancer Cells to Cisplatin through DNA Damage Assessment.
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Abdulghany, Zaynab Saad
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METFORMIN , *COMBINATION drug therapy , *GLIOMAS , *CISPLATIN , *AGAR , *CELL physiology , *CELL lines , *CANCER chemotherapy , *EXPERIMENTAL design , *DNA damage , *DRUG efficacy , *ELECTROPHORESIS , *CELL survival , *DRUG synergism , *PHARMACODYNAMICS - Abstract
Background: Glioblastoma (GBM) stands out as the most prevalent primary brain tumor characterized by its high aggressiveness. Numerous therapeutic approaches have been employed, and the utility of combination therapies has been substantiated, particularly in GBM treatment. Cisplatin, an anticancer chemotherapeutic agent, is employed for the management of various malignancies, including GBM; however, it is associated with significant systemic toxicity. In the realm of combination therapy, metformin, a biguanide drug conventionally used as a first-line treatment for type 2 diabetes, has recently emerged as a valuable adjunct in the treatment of a diverse spectrum of tumors. This study aimed to elucidate the impact of metformin on sensitizing the human cerebral GBM cancer cell line (AMGM) to cisplatin chemotherapy by employing the comet assay as a means to assess DNA damage, thereby advocating the potential of metformin as an adjuvant for cisplatin-based therapy. Method: In this experimental study, the AMGM cell line was cultured and subsequently treated with either single-agent cisplatin, metformin, or a combination of both drugs. Cell viability was assessed through growth inhibition calculations. The Chou--Talalay analysis was used to assess the cooperative effect of this drug combination. Furthermore, DNA fragmentation was quantified using the alkaline comet assay technique. Results: The findings demonstrate that metformin significantly potentiates the therapeutic efficacy of cisplatin by synergistically inhibiting the growth of AMGM cells and reducing DNA damage. Conclusion: These results underscore the potential utility of metformin as a valuable adjunct in enhancing the clinical effectiveness of chemotherapy regimens. [ABSTRACT FROM AUTHOR]
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- 2024
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7. Production of enterocin P by a bioengineered Pichia pastoris to control pathogen bacteria.
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Heravi, Reza Majidzadeh, Tanhaeian, Abbas, and Pakbaten, Bahareh
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BIOENGINEERING , *ENTEROCINS , *PICHIA pastoris , *PATHOGENIC bacteria , *ELECTROPHORESIS , *AMINO acid sequence - Abstract
Enterocin P has been characterized in class IIa bacteriocins and showed a broad spectrum of antimicrobial activity. In this study, the nucleotide sequence of enterocin P transformed and expressed in Pichia pastoris genomically, and the antimicrobial activity of the extracted protein was evaluated on pathogenic bacteria. The synthetic sequence of Enterocin P was ligated into pPICZαA, an integrated vector, in the EcoRI - XbaI site. The recombinant plasmid was amplified in Escherichia coli strain DH5α and proved for ligation and probably mutation by sequencing. The linearized plasmid was electroporated into Pichia pastoris. The presence of recombinant protein in yeast cell extraction was verified by SDS-PAGE and the antimicrobial activity of the purified protein. Recombinant Enterocin P tested for the inhibition of 8 avian pathogen bacteria via MIC test. Electrophoresis of purified protein and cell culture supernatant showed a protein band of about 5.4 kDa. The mass spectrometry of the sample exhibits an Amino acid sequence that corresponds to enterocin P in Enterococcus faecium with an accession number of OTN91526.1. Recombinant bacteriocin MIC test shows the most inhibition on Salmonella typhimurium and E. coli O157. Recombinant Pichia pastoris exhibited a potential for producing enterocin P in high concentrations; therefore, this antimicrobial peptide can be studied for pharmacological use or industrial applications. [ABSTRACT FROM AUTHOR]
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- 2024
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8. Analytical Techniques for Characterizing Tumor-Targeted Antibody-Functionalized Nanoparticles.
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Marques, Ana Camila, Costa, Paulo C., Velho, Sérgia, and Amaral, Maria Helena
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IMMUNOASSAY , *CELL receptors , *NANOPARTICLES , *RECEPTOR antibodies - Abstract
The specific interaction between cell surface receptors and corresponding antibodies has driven opportunities for developing targeted cancer therapies using nanoparticle systems. It is challenging to design and develop such targeted nanomedicines using antibody ligands, as the final nanoconjugate's specificity hinges on the cohesive functioning of its components. The multicomponent nature of antibody-conjugated nanoparticles also complicates the characterization process. Regardless of the type of nanoparticle, it is essential to perform physicochemical characterization to establish a solid foundation of knowledge and develop suitable preclinical studies. A meaningful physicochemical evaluation of antibody-conjugated nanoparticles should include determining the quantity and orientation of the antibodies, confirming the antibodies' integrity following attachment, and assessing the immunoreactivity of the obtained nanoconjugates. In this review, the authors describe the various techniques (electrophoresis, spectroscopy, colorimetric assays, immunoassays, etc.) used to analyze the physicochemical properties of nanoparticles functionalized with antibodies and discuss the main results. [ABSTRACT FROM AUTHOR]
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- 2024
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9. Next-generation sequencing in laboratory medicine.
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Chandra, Rajasri
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RNA analysis , *DNA analysis , *GENOME-wide association studies , *POLYMERASE chain reaction , *GENE expression , *PATHOLOGICAL laboratories , *ELECTROPHORESIS , *SEQUENCE analysis - Abstract
The article focuses on the impact of next-generation sequencing (NGS) technology in laboratory medicine, which has revolutionized disease diagnosis, prognosis, therapeutic decisions, and patient follow-up. It discusses the limitations of Sanger sequencing, which led to the development of NGS technologies capable of sequencing large numbers of DNA sequences simultaneously, offering faster and less expensive options for clinical applications.
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- 2024
10. Finite ion size effects on electrophoresis of a dielectric surfactant-laden droplet in a non-dilute electrolyte.
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Majhi, Subrata and Bhattacharyya, Somnath
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SURFACE charges , *ELECTROPHORESIS , *SURFACE potential , *DIELECTRICS , *ELECTROLYTES , *CAPILLARY electrophoresis , *CONCENTRATION gradient - Abstract
The ion steric repulsion and ion-solvent interactions, due to the finite size of ions, are analyzed for electrophoresis of a droplet in a non-dilute electrolyte in which the ionic volume fraction is O (0.01). In this study, the viscosity of the suspension medium is considered to be dependent on the local ionic volume fraction. The electrochemical potential of the finite-sized ions is modified to take into account the steric interactions. The impact of the Marangoni stress due to the non-uniform distribution of the non-ionized surfactant is taken into account. Governing equations are solved under a weak applied field consideration. At a lower range of surface charge density, the mobility is found to increase with the droplet viscosity, in which the Marangoni stress is found to enhance the mobility. The counterion saturation created by the ion steric interaction attenuates the screening of the surface charge and hence, enhances the mobility. However, the enhanced viscosity of the medium for the non-dilute electrolyte reduces the mobility and this reduction is higher in a monovalent electrolyte as compared to a multivalent electrolyte. The standard model shows a singularity in mobility when the surface potential is varied. However, the dependence of mobility on surface potential in the modified model is found to exhibit a smooth variation. • Modeling electrophoresis of a surfactant-laden highly charged viscous droplet. • Standard model is extended to include the finite ion size effects. • Ion steric interactions and dependence of viscosity on ionic concentration is included. • Marangoni stress due to surfactant concentration gradient is analyzed. • The limitation of the point-charge based standard model is highlighted. [ABSTRACT FROM AUTHOR]
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- 2024
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11. Interplay of electrokinetic effects in nonpolar solvents for electronic paper displays.
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Khorsand Ahmadi, Mohammad, Liu, Wei, Groenewold, Jan, den Toonder, Jaap M.J., Henzen, Alex, and Wyss, Hans M.
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INFORMATION display systems , *ELECTRONIC paper , *SOLVENTS , *DIELECTROPHORESIS , *ELECTRO-osmosis , *PROPERTIES of fluids - Abstract
Electronic paper displays rely on electrokinetic effects in nonpolar solvents to drive the displacement of colloidal particles within a fluidic cell. While Electrophoresis (EP) is a well-established and frequently employed phenomenon, electro-osmosis (EO), which drives fluid flow along charged solid surfaces, has not been studied as extensively. We hypothesize that by exploiting the interplay between these effects, an enhanced particle transport can be achieved. In this study, we experimentally investigate the combined effects of EP and EO for colloidal particles in non-polar solvents, driven by an electric field. We use astigmatism micro-particle tracking velocimetry (A- μ PTV) to measure the motion of charged particles within model fluidic cells. Using a simple approach that relies on basic fluid flow properties we extract the contributions due to EP and EO, finding that EO contributes significantly to particle transport. The validity of our approach is confirmed by measurements on particles with different magnitudes of charge, and by comparison to numerical simulations. We find that EO flows can play a dominant role in the transport of particles in electrokinetic display devices. This can be exploited to speed up particle transport, potentially yielding displays with significantly faster switching times. Interplay of electrokinetic effects : Driven by the applied electric field, both electro-osmosis (EO, top left), which drives a flow along the surfaces of the fluidic cell, and electrophoresis (EP, top right), which drives a migration of particles relative to the background liquid, occur in our system. A typical example of the resultant particle velocity field (bottom) illustrates the important role of EO on particle transport in the fluidic cell. • Studied electrokinetics of particles in nonpolar solvent using astigmatism micro-PTV. • Both electrophoresis (EP) and electro-osmosis (EO) play important roles. • Combination of EO and EP could be exploited in faster switching e-paper displays. [ABSTRACT FROM AUTHOR]
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- 2024
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12. Comparative study of the vibrating capillary nebulizer (VCN) and commercially available interfaces for on-line coupling of capillary electrophoresis with ICP-MS.
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Taylor, Tristen L., Tukhmetova, Dariya, Duong, Thi Phuong Thanh, Böwe, Anna-Maria, Meermann, Björn, and Gundlach-Graham, Alexander
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INDUCTIVELY coupled plasma mass spectrometry , *CAPILLARY electrophoresis , *NEBULIZERS & vaporizers , *CARBONACEOUS aerosols - Abstract
Capillary electrophoresis (CE) is a powerful and sensitive tool for speciation analysis when combined with inductively coupled plasma mass spectrometry (ICP-MS); however, the performance of this technique can be limited by the nature of pneumatic nebulizers. This study compares two commercially available pneumatic nebulizers to a newly introduced vibrating capillary nebulizer (VCN) for on-line coupling of CE with ICP-MS. The VCN is a low-cost, non-pneumatic nebulizer that is based on the design of capillary vibrating sharp-edge spray ionization. As a piezoelectrically driven nebulization source, the VCN creates an aerosol independent of gas flows and does not produce a low-pressure region at the nebulizer orifice. To compare the systems, we performed replicate analyses of sulfate in river water with each nebulizer and the same CE and ICP-MS instruments and determined the figures of merit of each setup. With the CE-VCN-ICP-MS setup, we achieved around 2–4 times lower sensitivity compared to the commercial setups. However, the VCN-based setup provided lower noise levels and better linear correlation from the analysis of calibration standards, which resulted in indistinguishable LOD and LOQ values from the in-house-built VCN-based and commercial setups for CE-ICP-MS analysis. The VCN is found to have the highest baseline stability with a standard deviation of 3500 cts s−1, corresponding to an RSD of 2.7%. High reproducibility is found with the VCN with a peak area RSD of 4.1% between 3 replicate measurements. [ABSTRACT FROM AUTHOR]
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- 2024
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13. TrichomeLess Regulator 3 is required for trichome initial and cuticle biosynthesis in Artemisia annua.
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Dong, Boran, Xu, Zihan, Wang, Xingxing, Li, JinXing, Xiao, Ying, Huang, Doudou, Lv, Zongyou, and Chen, Wansheng
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ARTEMISIA annua , *ARTEMISININ , *ELECTROPHORESIS , *ARABIDOPSIS thaliana , *TRANSCRIPTION factors - Abstract
Artemisinin is primarily synthesized and stored in the subepidermal space of the glandular trichomes of Artemisia annua. The augmentation of trichome density has been demonstrated to enhance artemisinin yield. However, existing literature lacks insights into the correlation between the stratum corneum and trichomes. This study aims to unravel the involvement of TrichomeLess Regulator 3 (TLR3), which encodes the transcription factor, in artemisinin biosynthesis and its potential association with the stratum corneum. TLR3 was identified as a candidate gene through transcriptome analysis. The role of TLR3 in trichome development and morphology was investigated using yeast two-hybrid, pull-down analysis, and RNA electrophoresis mobility assay. Our research revealed that TLR3 negatively regulates trichome development. It modulates the morphology of Arabidopsis thaliana trichomes by inhibiting branching and inducing the formation of abnormal trichomes in Artemisia annua. Overexpression of the TLR3 gene disrupts the arrangement of the stratum corneum and reduces artemisinin content. Simultaneously, TLR3 possesses the capacity to regulate stratum corneum development and trichome follicle morphology by interacting with TRICHOME AND ARTEMISININ REGULATOR 1, and CycTL. Consequently, our findings underscore the pivotal role of TLR3 in the development of glandular trichomes and stratum corneum biosynthesis, thereby influencing the morphology of Artemisia annua trichomes. [ABSTRACT FROM AUTHOR]
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- 2024
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14. Diffusiophoresis of Macromolecules within the Framework of Multicomponent Diffusion.
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Annunziata, Onofrio
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CONCENTRATION gradient , *NERNST-Planck equation , *DIFFUSION coefficients , *MACROMOLECULES , *POLYETHYLENE glycol , *LYSOZYMES - Abstract
Diffusiophoresis is the isothermal migration of a colloidal particle through a liquid caused by a cosolute concentration gradient. Although diffusiophoresis was originally introduced using hydrodynamics, it can also be described by employing the framework of multicomponent diffusion. This not only enables the extraction of diffusiophoresis coefficients from measured multicomponent-diffusion coefficients but also their theoretical interpretation using fundamental thermodynamic and transport parameters. This review discusses the connection of diffusiophoresis with the 2 × 2 diffusion-coefficient matrix of ternary liquid mixtures. Specifically, diffusiophoresis is linked to the cross-term diffusion coefficient characterizing diffusion of colloidal particles due to cosolute concentration gradient. The other cross-term, which describes cosolute diffusion due to the concentration gradient of colloidal particles, is denoted as osmotic diffusion. Representative experimental results on diffusiophoresis and osmotic diffusion for polyethylene glycol and lysozyme in the presence of aqueous salts and osmolytes are described. These data were extracted from ternary diffusion coefficients measured using precision Rayleigh interferometry at 25 °C. The preferential-hydration and electrophoretic mechanisms responsible for diffusiophoresis are examined. The connection of diffusiophoresis and osmotic diffusion to preferential-interaction coefficients, Onsager reciprocal relations, Donnan equilibrium and Nernst–Planck equations are also discussed. [ABSTRACT FROM AUTHOR]
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- 2024
- Full Text
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15. Control of Dielectric Parameters of Micro- and Nanomodified Epoxy Resin Using Electrophoresis.
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Dąda, Anna, Błaut, Paweł, Mikrut, Paweł, Kuniewski, Maciej, and Zydroń, Paweł
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EPOXY resins , *BROADBAND dielectric spectroscopy , *DIELECTRICS , *ELECTROPHORESIS , *PERMITTIVITY , *DIELECTRIC loss , *ELECTROPHORETIC deposition , *DIELECTROPHORESIS - Abstract
This work presents the results of research on submicro- and nanocomposites with gradient properties, produced in a planned electrophoretic process. Epoxy-resin-based samples were filled with TiO2 particles of three different sizes (13 nm, 38 nm, and <1 µm) at four different values of average electric field Eav (0.0 Vmm−1, 125 Vmm−1, 250 Vmm−1, and 500 Vmm−1) for 1 h each. Changes in selected dielectric parameters (dielectric constant εr and dielectric loss factor tanδ) of the composites were analyzed using broadband dielectric spectroscopy (10−1 Hz to 105 Hz). The influence of the Eav and the current i(t) flowing through the sample material and the Joule heat generated in it on the resin curing process and the final gradient of dielectric parameters were investigated. The results show that the degree of modification of the εr gradient increases with increasing Eav and is more pronounced in the case of TiO2 nanoparticles. The largest modifications in the εr and tanδ were obtained for nanoparticles with a diameter of 13 nm at Eav = 500 Vmm−1, while the lowest for particles < 1 µm at Eav = 125 Vmm−1. The effect of electrophoresis on the dielectric parameters is significant, especially near the anode region. Increasing the concentration of TiO2 particles at the anode occurs at the expense of reducing their concentration in the remaining volume of the sample. The test results clearly demonstrate the importance of particle dimension and electric field strength for the gradient modification of the properties of the epoxy composite using electrophoresis. Numerical simulations of electric field stresses in the epoxy resin during the electrophoresis process, performed in the COMSOL program, revealed a significant increase in the E field strength in the areas close to the anode and cathode. [ABSTRACT FROM AUTHOR]
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- 2024
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16. Concise Analysis of Single-Stranded DNA of Recombinant Adeno-Associated Virus By Automated Electrophoresis System.
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Yuan, Yuzhe, Higashiyama, Kiyoko, Hashiba, Noriko, Masumi-Koizumi, Kyoko, Yusa, Keisuke, and Uchida, Kazuhisa
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DNA analysis , *ADENO-associated virus , *RECOMBINANT DNA , *RECOMBINANT viruses , *ELECTROPHORESIS , *SINGLE-stranded DNA - Abstract
Recombinant adeno-associated virus (rAAV) is a prominent viral vector currently available for human gene therapy. The diameter of the rAAV capsid is ∼25 nm, and a positive or negative single-stranded DNA is packaged within the vector capsid. In this report, we describe a concise method to examine the extracted rAAV genome using an automated electrophoresis system. The rAAV genome, prepared from vector particles through either heat treatment at 95°C for 10 min or the phenol–chloroform extraction method, was analyzed using an automated electrophoresis system under denaturation conditions. The heat treatment protocol demonstrated a comparable yield with the phenol–chloroform extraction protocol, and the quantified amounts of the rAAV genome obtained using the automated electrophoresis system were consistent with those quantitated by quantitative PCR. Additionally, crude rAAV extractions could also be analyzed by the automated electrophoresis system after DNase I treatment. These results indicated that this simple and quick analysis using automated electrophoresis is highly useful for confirming the purity and integrity of the rAAV genome. [ABSTRACT FROM AUTHOR]
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- 2024
- Full Text
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17. Monoclonal Gammopathy of Renal Significance (MGRS) - Case Series from a Tertiary Center in Kerala.
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Padmanabhan, Priya, Jayameena P., Mampilly, Neena, Francis, Shinto, Sherif, Ajmal, George, Sunil, and Mathew, Thomas
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PROTEINURIA , *PARAPROTEINEMIA , *PHYSICAL diagnosis , *KIDNEY failure , *CHRONIC lymphocytic leukemia , *CARDIO-renal syndrome , *TERTIARY care , *NEPHROTIC syndrome , *MONOCLONAL gammopathies , *KIDNEY diseases , *ELECTROPHORESIS - Abstract
Monoclonal gammopathy of renal significance (MGRS) has gained importance because identifying the monoclonal deposit and addressing it, rather than treating renal dysfunction as the primary pathology, has salvaged the patients from progressing into end-stage renal disease. Since it affects elderly population, there could be a propensity to misdiagnose them with cardiorenal syndrome. We present four patients of MGRS diagnosed from our center. They presented with proteinuria or unexplained renal dysfunction. Three of the patients were diagnosed to have amyloidosis, of which two had lambda-type and one had kappa amyloidosis. The fourth patient had fibrillary glomerulonephritis with kappa restriction, further evaluation of which led to diagnosis of chronic lymphocytic leukemia. Absence of "M" band in protein electrophoresis and a normal bone marrow study should not stop physicians from further evaluation. Quantitative serum immunofixation electrophoresis and electron microscopic examination of renal biopsy have become a comprehensive diagnostic tool in such patients. [ABSTRACT FROM AUTHOR]
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- 2024
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18. Trans-2-nonadecyl-4-(hydroxymethyl)-1,3-dioxolane (TNHD) purified from freshwater clams markedly alleviates dimethylnitrosamine-induced hepatic fibrosis.
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Ya-Ru Kuo, Chen-Yu Tsai, Wei-Sheng Lin, Chi-I Chang, Ching-Shu Lai, and Min-Hsiung Pan
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BIOLOGICAL models , *MITOGEN-activated protein kinases , *HIGH performance liquid chromatography , *HEPATOTOXICOLOGY , *MUSCLE proteins , *PROLINE , *CARRIER proteins , *T-test (Statistics) , *PHOSPHORYLATION , *RESEARCH funding , *NECROSIS , *BODY weight , *ENZYMES , *CELLULAR signal transduction , *REVERSE transcriptase polymerase chain reaction , *DESCRIPTIVE statistics , *FIBROSIS , *LIVER diseases , *RATS , *CELL lines , *REACTIVE oxygen species , *MICE , *GENE expression , *ANIMAL experimentation , *WESTERN immunoblotting , *MOLECULAR structure , *MOLLUSKS , *TISSUE extracts , *INFLAMMATION , *TRIGLYCERIDES , *CYTOKINES , *EXTRACELLULAR matrix , *STAINS & staining (Microscopy) , *ELECTROPHORESIS , *TRANSFORMING growth factors-beta , *BIOMARKERS - Abstract
Liver fibrosis occurs due to injury or inflammation, which results in the excessive production of collagen and the formation of fibrotic scar tissue that impairs liver function. Despite the limited treatment options available, freshwater clams may hold promise in the treatment of liver fibrosis. In this study, we demonstrated the effects of ethanol extract of freshwater clam (FCE), ethyl acetate extract of FCE (EA-FCE), and trans-2-nonadecyl-4-(hydroxymethyl)-1,3-dioxolane (TNHD) on liver fibrosis induced by dimethylnitrosamine (DMN). Administration of FCE and TNHD alleviated liver injury, including tissue damage, necrosis, inflammation scores, fibrosis scores, serum enzymes, and triglyceride levels. Furthermore, we analyzed the expression of fibrosis-related proteins, such as a-smooth muscle actin (a-SMA) and transforming growth factor (TGF-b), as well as the hydroxyproline content, which decreased after treatment with FCE and TNHD. Animal experiments revealed that FCE and TNHD can reduce liver fibrosis by inhibiting cytokines that activate stellate cells and decreasing extracellular matrix (ECM) secretion. Cell experiments have shown that TNHD inhibits the MAPK/Smad signaling pathway and TGF-b1 activation, resulting in a reduction in the expression of fibrosisrelated proteins. Therefore, freshwater clam extracts, particularly TNHD, may have potential therapeutic and preventive effects for the amelioration of liver fibrosis. [ABSTRACT FROM AUTHOR]
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- 2024
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19. Overview on Antioxidant and Oxidative Stress Markers after Garlic Oil Supplement in Suckling Buffalo Calves.
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YOUSIF, Hamada Mahmoud, EL MAHDY, Ahmed Mohamed, HASSAN, Marwa Fouad, and MANSOUR, Mogda Kamel
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OXIDATIVE stress , *CALVES , *GARLIC , *IMMUNOREGULATION , *VITAMIN E , *SELENOPROTEINS , *VITAMIN A - Abstract
Calves during the suckling period have serious attention and induce immense bearing on early maturity and production. This work aimed to scrutinize the affections of garlic oil supplements on antioxidants, oxidative stress, and immune response in suckling buffalo calves. To achieve this aim we used twenty suckling buffalo calves, and divided them into two groups. The first group (n=10) did not receive any supplement, and the second group (n=10) received garlic oil as a supplement 5 mL/calf/day orally for 7 days. Whole blood and serum samples were collected on the 1st, 3rd, 5th, and 7th day of supplement from all calves. Our data showed that the supplemented garlic oil significantly increase lysated blood and serum SOD, CAT, GSH, and TAC levels, while a gradual decrease in MDA and non-significant changes in NO as indicators of oxidative stress, associated with a significant increase in serum vitamins A and E, selenium, iron, and zinc. Total protein, albumin, globulin, γ1, γ2, α1, α2 were gradually and significantly increased in comparison with the non-supplemented control group. In conclusion, garlic oil supplement have promotives effects on enzymatic and non-enzymatic antioxidant system, immunity, nutrient utilization required for immunity modulation and regulation in calves. [ABSTRACT FROM AUTHOR]
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- 2024
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20. Evaluation of Flexible Electrochromic Device Based on V2O5 Film Prepared by Electrophoresis.
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Huang, Shenghuai, Yang, Mingqing, Zhang, Shiyu, Wang, Lei, Niu, Chunhui, and Lv, Yong
- Abstract
The red V2O5 sol was prepared by facile alternating stirring and ultrasonic dispersion route. A V2O5 film fabricated on ITO-PET by electrophoresis method was used to demonstrate a flexible electrochromic device. The effects of applied voltage and electrophoresis time for V2O5films were investigated in detail. When the electrophoresis time was kept 30 s, the applied voltage was above 3 V to deposit uniform V2O5-PET film. The electrophoresis time could more significantly regulate the deposition of V2O5 on ITO-PET film. A flexible electrochromic device with “sandwich” structure was fabricated using V2O5 film, and its electrochromic performance of the device was evaluated. The flexible V2O5 electrochromic devices show the multicolor electrochromic performance of yellow, yellow-green, green and orange-red. The coloration efficiency values of V2O5 devices were 21.6 cm2C−1 (from initial yellow to green) and 26.9 cm2C−1 (from green to orange-red), respectively, and the highest transmittance modulation range was 51% at 741 nm. The work provided a facile and green method for fabrication of flexible V2O5 electrochromic device. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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21. Understanding the Constraints and Optimization of Serum Immunofixation Electrophoresis and Serum Free Light Chains for Detecting Monoclonal Proteins: A Single-Center Experience.
- Author
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Shastri, Malvika, Malhotra, Pankaj, Kaur, Harvinder, and Aggarwal, Ritu
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IMMUNOGLOBULIN light chains , *MONOCLONAL antibodies , *ELECTROPHORESIS , *MULTIPLE myeloma , *PROTEINS - Abstract
Introduction Serum immunofixation electrophoresis (SIFE) and serum free light chain (SFLC) assay are imperative investigations in diagnosis and follow-up of multiple myeloma (MM). SFLC assays are reported to have higher sensitivity than SIFE. However, discrepancies have been reported between them. The current study was aimed at assessing concordance and discordance between SIFE and SFLC results in MM. Methods A total of 450 observations of both SIFE and SFLC were obtained from treatment-naive and follow-up MM patients. Results One hundred and twenty-nine (28.7%) values were observed as discordant, that is, positive SIFE with normal SFLC ratio or negative SIFE with abnormal SFLC ratio (p -value < 0.00001). Proportion of discordance was higher in SIFE positive-SFLC normal cases than SIFE negative-SFLC abnormal cases. Discordance was more frequent in follow-up cases. Conclusion Negative SFLC alone may not be reliable for MM follow-up. Algorithm may be based on SFLC measurements on each follow-up till attainment of normal SFLC ratio. Once SFLC normalizes, follow-up may be done with SIFE. If SIFE is positive, further follow-up with SIFE may be initiated. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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22. Bioengineered Small-Diameter Vascular Xenografts as an Alternative to Autologous Vascular Grafting for Emergency Revascularization – a Preliminary Study.
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Harpa, Marius Mihai, Oltean, Sânziana Flămând, Puscas, Alexandra Iulia, Truta, Raluca, Anitei, David Emanuel, Ghiragosian, Claudiu, Banceu, Cosmin, Movileanu, Ionela, Cotoi, Ovidiu Simion, Niculescu, Raluca, Suciu, Horatiu, Simionescu, Dan, and Hussein, Hussam Al
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XENOGRAFTS , *REVASCULARIZATION (Surgery) , *EMERGENCY medical services , *DNA analysis , *ELECTROPHORESIS - Abstract
Autologous vascular arterial or venous graft are not available in 10-40% of patients, due to vascular pathologies, and the utility of decellularized biological scaffolds would be a solution for those cases. The purpose of this research was to obtain a functional acellular xenograft, prior to in-vivo testing as a vascular graft in an experimental animal. Two batches of carotid vasculo-nervous bundles were collected from porcine models from a local slaughterhouse. The arterial grafts were dissected and isolated, obtaining carotid arteries with a caliber of 5–6 mm and a length of approximately 10–12 cm. Two decellularization protocols were used, immersion (n = 10) and perfusion (n = 9). The resulting grafts underwent histological examination, DNA analysis, electrophoresis and spectrophotometry. Due to severe tissue damage and friability, the batch that was decellularized using perfusion was not examined. The histological examination of grafts stained with hematoxylin-eosin and DAPI highlighted the absence of nuclei. Spectrophotometry revealed a 90% decellularization, and electrophoresis of revealed the migration band of the material extracted from the fresh tissue, as well as the absence of migration bands in the case of the material extracted from decellularized tissues. We successfully used the immersion protocol to obtain a functional acellular vascular graft, in contrast to perfusion decellularization, where intraluminal high pressures damage the extracellular matrix. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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23. Technological Processing of Dried Powdered Rosehips to Tablets Through Wet Granulation.
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Špaglová, M., Matušková, M., Lawson, M.K., Čuchorová, M., Čierna, M., Krchňák, D., Mikušová, V., Piešťanský, J., and Mikuš, P.
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DRUG tablets , *LACTOSE , *HEAT , *MEDICINAL plants , *ELECTROPHORESIS , *VITAMIN C , *PASSIFLORA , *PROPIONIC acid , *QUALITY assurance , *MINERAL industries , *PLANT extracts , *CELLULOSE , *PHARMACEUTICAL chemistry , *OXALIC acid , *STATISTICAL sampling , *DOSAGE forms of drugs , *POWDERS - Abstract
The pseudo-fruits of Dog Rose are a rich source of L-ascorbic acid and several other active substances, which means their high supportive therapeutic potential. The study aimed to examine the impact of the chosen technological procedure for the preparation of tablets containing rosehip powder on the amount of L-ascorbic acid in the final pharmaceutical form. Drying of the plant drug was performed at room temperature to avoid possible thermal degradation of this heat-sensitive compound. Similarly, drying of the granules after wet granulation in the oven was replaced by natural drying at room temperature. The composition of two types of prepared granule formulations differed in the filler – lactose (LAC) or microcrystalline cellulose (MCC). Apart from the disintegration test, they meet the technological requirements for granules or tablets. Lactose was confirmed as a more suitable filler, which despite the unsuccessful disintegration of the granules, ensures the disintegration of tablets within 15 minutes even without the addition of a special excipient acting as a disintegrant. The content of L-ascorbic acid detected using isotachophoresis – capillary zone electrophoresis was 87.16 ± 5.06 µg in LAC tablets and 63.33 ± 2.83 µg in MCC tablets. [ABSTRACT FROM AUTHOR]
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- 2023
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24. Influence of Amino Acid Substitutions in ApoMb on Different Stages of Unfolding of Amyloids.
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Katina, Natalya, Marchenkov, Victor, Ryabova, Natalya, Ilyina, Nelly, Marchenko, Natalia, Balobanov, Vitalii, and Finkelstein, Alexey
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AMYLOID beta-protein , *AMINO acids , *AMYLOID , *SOIL structure , *MONOMERS , *ELECTROPHORESIS - Abstract
To date, most research on amyloid aggregation has focused on describing the structure of amyloids and the kinetics of their formation, while the conformational stability of fibrils remains insufficiently explored. The aim of this work was to investigate the effect of amino acid substitutions on the stability of apomyoglobin (ApoMb) amyloids. A study of the amyloid unfolding of ApoMb and its six mutant variants by urea has been carried out. Changes in the structural features of aggregates during unfolding were recorded by far-UV CD and native electrophoresis. It was shown that during the initial stage of denaturation, amyloids' secondary structure partially unfolds. Then, the fibrils undergo dissociation and form intermediate aggregates weighing approximately 1 MDa, which at the last stage of unfolding decompose into 18 kDa monomeric unfolded molecules. The results of unfolding transitions suggest that the stability of the studied amyloids relative to the intermediate aggregates and of the latter relative to unfolded monomers is higher for ApoMb variants with substitutions that increase the hydrophobicity of the residues. The results presented provide a new insight into the mechanism of stabilization of protein aggregates and can serve as a base for further investigations of the amyloids' stability. [ABSTRACT FROM AUTHOR]
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- 2023
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25. Association of Air Pollution with a Urinary Biomarker of Biological Aging and Effect Modification by Vitamin K in the FLEMENGHO Prospective Population Study.
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Martens, Dries S., De-Wei An, Yu-Ling Yu, Chori, Babangida S., Congrong Wang, Silva, Ana Inês, Fang-Fei Wei, Chen Liu, Stolarz-Skrzypek, Katarzyna, Rajzer, Marek, Latosinska, Agnieszka, Mischak, Harald, Staessen, Jan A., and Nawrot, Tim S.
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LIPID metabolism , *KIDNEY physiology , *AIR pollution , *BIOMARKERS , *MATRIX Gla protein , *CONFIDENCE intervals , *ELECTROPHORESIS , *CARBON , *INFLAMMATION , *MORTALITY , *REGRESSION analysis , *RISK assessment , *PROTEOMICS , *AGING , *MASS spectrometry , *DESCRIPTIVE statistics , *DATA analysis software , *VITAMIN K , *ENVIRONMENTAL exposure , *PROPORTIONAL hazards models , *LONGITUDINAL method - Abstract
BACKGROUND: A recently developed urinary peptidomics biological aging clock can be used to study accelerated human aging. From 1990 to 2019, exposure to airborne particulate matter (PM) became the leading environmental risk factor worldwide. OBJECTIVES: This study investigated whether air pollution exposure is associated with accelerated urinary peptidomic aging, independent of calendar age, and whether this association is modified by other risk factors. METHODS: In a Flemish population, the urinary peptidomic profile (UPP) age (UPP-age) was derived from the urinary peptidomic profile measured by capillary electrophoresis coupled with mass spectrometry. UPP-age-R was calculated as the residual of the regression of UPP-age on chronological age, which reflects accelerated aging predicted by UPP-age, independent of chronological age. A high-resolution spatial-temporal interpolation method was used to assess each individual’s exposure to PM10, PM2.5, black carbon (BC), and nitrogen dioxide (NO2). Associations of UPP-age-R with these pollutants were investigated by mixed models, accounting for clustering by residential address and confounders. Effect modifiers of the associations between UPP-age-R and air pollutants that included 18 factors reflecting vascular function, renal function, insulin resistance, lipid metabolism, or inflammation were evaluated. Direct and indirect (via UPP-age-R) effects of air pollution on mortality were evaluated by multivariable adjusted Cox models. RESULTS: Among 660 participants (50.2% women; mean age: 50.7 y), higher exposure to PM10, PM2.5, BC, and NO2 was associated with a higher UPP-age-R. Studying effect modifiers showed that higher plasma levels of desphospho-uncarboxylated matrix Gla protein (dpucMGP), signifying poorer vitamin K status, steepened the slopes of UPP-age-R on the air pollutants. In further analyses among participants with dpucMGP =4.26 μg/L (median), an interquartile range (IQR) higher level in PM10, PM2.5, BC, and NO2 was associated with a higher UPP-age- R of 2.03 [95% confidence interval (CI): 0.60, 3.46], 2.22 (95% CI: 0.71, 3.74), 2.00 (95% CI: 0.56, 3.43), and 2.09 (95% CI: 0.77, 3.41) y, respectively. UPP-age-R was an indirect mediator of the associations of mortality with the air pollutants [multivariable-adjusted hazard ratios from 1.094 (95% CI: 1.000, 1.196) to 1.110 (95% CI: 1.007, 1.224)] in participants with a high dpucMGP, whereas no direct associations were observed. DISCUSSION: Ambient air pollution was associated with accelerated urinary peptidomics aging, and high vitamin K status showed a potential protective effect in this population. Current guidelines are insufficient to decrease the adverse health effects of airborne pollutants, including healthy aging trajectories. [ABSTRACT FROM AUTHOR]
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- 2023
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26. Evaluation of the serum tRNA-derived fragment tRF-5022B as a potential biomarker for the diagnosis of osteoarthritis.
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Ni, Yingchen, Wu, Anqi, Li, Jianxin, Zhang, Weidong, and Wang, Youhua
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OSTEOARTHRITIS diagnosis , *REVERSE transcriptase polymerase chain reaction , *DISEASE progression , *TRANSFER RNA , *SEQUENCE analysis , *ELECTROPHORESIS , *GENE expression , *GENETIC markers , *AGAR , *RESEARCH funding , *SENSITIVITY & specificity (Statistics) , *RECEIVER operating characteristic curves - Abstract
Osteoarthritis (OA) is a degenerative disease. It is common in middle-aged and elderly people and is one of the main causes of disability. Currently, the etiology of OA is unclear, and no specific biomarkers for the diagnosis of OA have been identified. Therefore, finding a highly sensitive biomarker is essential for a proper diagnosis. TRNA-derived fragments (tRFs) and tRNA-derived stress-induced RNAs (tiRNAs) are newly discovered classes of noncoding RNAs. tRF has been proven in several studies to have significant associations with tumor diagnosis, making it a promising biomarker in cancer research. However, the diagnostic utility of tRF in OA patients and the correlation between OA progression and trf differential expression have yet to be elaborated. The purpose of this research was to identify tRFs with differential expression in OA to assess their potential as OA biomarkers. To determine the tRF-5022B expression level in this research, real-time fluorescence quantitative PCR has been employed. Agarose gel electrophoresis, Sanger sequencing, and other investigations have been employed for evaluating tRF-5022B's molecular properties. Receiver operating characteristic curve analysis has been utilized for assessing the diagnostic effectiveness of the tRF-5022B. The findings demonstrated that tRF-5022B expression was considerably lower in OA serum. The Kellgren–Lawrence grading scale was shown to correspond with serum expression levels. The ROC curve confirmed that tRF-5022B serum expression levels might differentiate OA cases from healthy individuals and RA patients. According to the aforementioned findings, tRF-5022B may be employed as a novel biomarker for OA diagnosis due to its excellent diagnostic value. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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27. Evaluation of Neutralization Potential of Naja naja and Daboia russelii Snake Venom by Root Extract of Cyanthillium cinereum.
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Suji S., Dinesh M. D., Keerthi K. U., Anagha K. P., Arya J., and Anju K. V.
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PHYTOTHERAPY , *SNAKE venom , *IN vitro studies , *MEDICINAL plants , *HEMOLYSIS & hemolysins , *ELECTROPHORESIS , *ORGANIC compounds , *PROTEOLYTIC enzymes , *PHYTOCHEMICALS , *GAS chromatography , *QUALITATIVE research , *ANTIVENINS , *MASS spectrometry , *PLANT extracts - Abstract
Aim: One of the main reasons for the death due to snake bites is the non-availability of antivenoms in the regions where they are needed. The use of medicinal plants and plant-based natural products as an alternative to antivenom will become a milestone in snake bite envenomation. The present study investigates the in vitro antivenom properties of Cyanthillium cinereum root extracts. Materials and methods: The C. cinereum root's aqueous extract was prepared by the Soxhlet extraction method, and phytochemical screening was performed to detect the presence of various bioactive compounds. Thin-layer chromatography (TLC) and gas chromatography--mass spectrometry (GC--MS) analysis were performed for the detection and identification of phytochemical constituents. In this study, an in vitro model is used to assess the antivenom capability of aqueous extract. Venom toxicity and neutralization assays were as follows: An in vitro pharmacological evaluation was performed by direct hemolysis assay, indirect hemolytic assay, proteolytic activity, neutralization of procoagulant activity, and gelatin liquefaction method. Results: Qualitative analysis of phytochemicals by the standard method showed the presence of various phytochemical constituents. Also, GC--MS analysis showed the presence of three major compounds that possess antivenom activity from the obtained 60 bioactive compounds, and their chemical structures were also determined. Venom protein profiling was performed by sodium dodecyl sulfate--polyacrylamide gel electrophoresis (SDS--PAGE) analysis. The plant extract was able to neutralize the Naja naja (N. naja) and Daboia russelii (D. russelii) venom induced hemolysis and it was reduced below 50 and 40%, respectively and the extract was also able to reduce the hemolytic halo produced by venoms. Procoagulant activity and gelatin liquefaction assay showed that venom-induced clotting was neutralized by increasing the root extract concentration sufficiently. Conclusion: The aqueous extract of the root of C. cinereum showed potent in vitro venom-neutralizing activity, and it can be used as a formidable therapeutic agent against N. naja and D. russelii envenomation. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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28. Proteomic-Based Discovery of Predictive Biomarkers for Drug Therapy Response and Personalized Medicine in Chronic Immune Thrombocytopenia.
- Author
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Gilanchi, Samira, Faranoush, Mohammad, Daskareh, Mahyar, Sadjjadi, Fatemeh Sadat, Zali, Hakimeh, Ghassempour, Alireza, and Rezaei Tavirani, Mostafa
- Subjects
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BIOMARKERS , *BLOOD proteins , *CHRONIC diseases , *ELECTROPHORESIS , *THROMBOPENIC purpura , *INDIVIDUALIZED medicine , *PEROXISOME proliferator-activated receptors , *PROTEOMICS , *TREATMENT effectiveness , *BIOINFORMATICS , *COMPARATIVE studies , *BLOOD platelet activation , *CELLULAR signal transduction , *ENZYME-linked immunosorbent assay , *RESEARCH funding , *MASS spectrometry , *INTESTINAL absorption , *CELL adhesion molecules , *PREDICTION models , *ONTOLOGIES (Information retrieval) - Abstract
Purpose. ITP is the most prevalent autoimmune blood disorder. The lack of predictive biomarkers for therapeutic response is a major challenge for physicians caring of chronic ITP patients. This study is aimed at identifying predictive biomarkers for drug therapy responses. Methods. 2D gel electrophoresis (2-DE) was performed to find differentially expressed proteins. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS) analysis was performed to identify protein spots. The Cytoscape software was employed to visualize and analyze the protein-protein interaction (PPI) network. Then, enzyme-linked immunosorbent assays (ELISA) were used to confirm the results of the proteins detected in the blood. The DAVID online software was used to explore the Gene Ontology and pathways involved in the disease. Results. Three proteins, including APOA1, GC, and TF, were identified as hub-bottlenecks and confirmed by ELISA. Enrichment analysis results showed the importance of several biological processes and pathway, such as the PPAR signaling pathway, complement and coagulation cascades, platelet activation, vitamin digestion and absorption, fat digestion and absorption, cell adhesion molecule binding, and receptor binding. Conclusion and Clinical Relevance. Our results indicate that plasma proteins (APOA1, GC, and TF) can be suitable biomarkers for the prognosis of the response to drug therapy in ITP patients. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
29. Evaluation of the serum tRNA-derived fragment tRF-5022B as a potential biomarker for the diagnosis of osteoarthritis.
- Author
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Ni, Yingchen, Wu, Anqi, Li, Jianxin, Zhang, Weidong, and Wang, Youhua
- Subjects
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OSTEOARTHRITIS diagnosis , *BIOMARKERS , *TRANSFER RNA , *ELECTROPHORESIS , *RESEARCH funding , *MOLECULAR structure , *RECEIVER operating characteristic curves - Abstract
Osteoarthritis (OA) is a degenerative disease. It is common in middle-aged and elderly people and is one of the main causes of disability. Currently, the etiology of OA is unclear, and no specific biomarkers for the diagnosis of OA have been identified. Therefore, finding a highly sensitive biomarker is essential for a proper diagnosis. TRNA-derived fragments (tRFs) and tRNA-derived stress-induced RNAs (tiRNAs) are newly discovered classes of noncoding RNAs. tRF has been proven in several studies to have significant associations with tumor diagnosis, making it a promising biomarker in cancer research. However, the diagnostic utility of tRF in OA patients and the correlation between OA progression and trf differential expression have yet to be elaborated. The purpose of this research was to identify tRFs with differential expression in OA to assess their potential as OA biomarkers. To determine the tRF-5022B expression level in this research, real-time fluorescence quantitative PCR has been employed. Agarose gel electrophoresis, Sanger sequencing, and other investigations have been employed for evaluating tRF-5022B's molecular properties. Receiver operating characteristic curve analysis has been utilized for assessing the diagnostic effectiveness of the tRF-5022B. The findings demonstrated that tRF-5022B expression was considerably lower in OA serum. The Kellgren–Lawrence grading scale was shown to correspond with serum expression levels. The ROC curve confirmed that tRF-5022B serum expression levels might differentiate OA cases from healthy individuals and RA patients. According to the aforementioned findings, tRF-5022B may be employed as a novel biomarker for OA diagnosis due to its excellent diagnostic value. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
30. A Novel Electrophoretic Technique to Improve Metasurface Sensing of Low Concentration Particles in Solution.
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Kurland, Zachary A. and Goyette, Thomas
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MILLIMETER wave devices , *ELECTRIC fields , *NANOPARTICLES , *ENVIRONMENTAL sciences - Abstract
A novel electrophoretic technique to improve metasurface sensing capabilities of charged particles in solution is presented. The proposed technique may improve the ability of metasurfaces to sense charged particles in solution in a manner not possible using the current standard of particle deposition (which allows particles to sediment randomly on a metasurface under evaporation) by inducing an external, nonuniform electric field through the metasurface apertures. Such a technique may be useful in various sensing applications, such as in biological, polymer, or environmental sciences, where low concentration particles in solution are of interest. The electrophoretic technique was simulated and experimentally tested using latex nanoparticles in solution. The results suggest that, using this technique, one may theoretically increase the particle density within the metasurface regions of greatest sensitivity by nearly 1900% in comparison to random sedimentation due to evaporation. Such an increase in particle density within the regions of greatest sensitivity may facilitate more precise material property measurements and enhance identification and detection capabilities of metasurfaces to particles in solution which constitute only a few hundred parts per million by mass. It was experimentally determined that the electrophoretic technique enhanced metasurface sensing capabilities of 333 parts per million by mass latex nanoparticle solutions by nearly 1700%. [ABSTRACT FROM AUTHOR]
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- 2023
- Full Text
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31. Exploring the World of Membrane Proteins: Techniques and Methods for Understanding Structure, Function, and Dynamics.
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Boulos, Imad, Jabbour, Joy, Khoury, Serena, Mikhael, Nehme, Tishkova, Victoria, Candoni, Nadine, Ghadieh, Hilda E., Veesler, Stéphane, Bassim, Youssef, Azar, Sami, and Harb, Frédéric
- Subjects
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MEMBRANE proteins , *NUCLEAR magnetic resonance spectroscopy , *BIOLOGICAL membranes , *X-ray crystallography , *HYDROPHOBIC interactions - Abstract
In eukaryotic cells, membrane proteins play a crucial role. They fall into three categories: intrinsic proteins, extrinsic proteins, and proteins that are essential to the human genome (30% of which is devoted to encoding them). Hydrophobic interactions inside the membrane serve to stabilize integral proteins, which span the lipid bilayer. This review investigates a number of computational and experimental methods used to study membrane proteins. It encompasses a variety of technologies, including electrophoresis, X-ray crystallography, cryogenic electron microscopy (cryo-EM), nuclear magnetic resonance spectroscopy (NMR), biophysical methods, computational methods, and artificial intelligence. The link between structure and function of membrane proteins has been better understood thanks to these approaches, which also hold great promise for future study in the field. The significance of fusing artificial intelligence with experimental data to improve our comprehension of membrane protein biology is also covered in this paper. This effort aims to shed light on the complexity of membrane protein biology by investigating a variety of experimental and computational methods. Overall, the goal of this review is to emphasize how crucial it is to understand the functions of membrane proteins in eukaryotic cells. It gives a general review of the numerous methods used to look into these crucial elements and highlights the demand for multidisciplinary approaches to advance our understanding. [ABSTRACT FROM AUTHOR]
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- 2023
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32. Comparison of the Serum Protein Electrophoretic Pattern and Concentrations of Acute Phase Proteins in Bitches with and Without Mammary Gland Tumors.
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Tóthová, Csilla, Valenčáková, Alexandra, Horňáková, Ľubica, and Nagy, Oskar
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ACUTE phase proteins , *BLOOD proteins , *MAMMARY glands , *FEMALE dogs , *HAPTOGLOBINS , *BLOOD protein electrophoresis , *ALBUMINS - Abstract
Alterations in the serum protein pattern may be associated with many diseases, including neoplastic processes. In veterinary medicine, these changes are poorly understood. Therefore, this study was aimed at the analysis of the distribution of blood serum protein fractions separated by agarose gel electrophoresis, and at the determination of the concentrations of main acute phase proteins in bitches with mammary gland neoplasia. The evaluation was conducted on twelve female dogs with palpable single or multiple nodules in the parenchyma of the mammary gland and on ten tumor-free clinically healthy bitches to compare the possible differences in the obtained results. Blood serum was used to perform agarose gel electrophoresis of the main blood serum protein fractions and to analyze the concentrations of total serum proteins and the following canine acute phase proteins: serum amyloid A, haptoglobin, C-reactive protein and α1-acid glycoprotein. The concentrations of total serum proteins were slightly higher in bitches with mammary gland tumors. Serum protein electrophoresis showed lower mean concentrations of albumin and α1-globulins in the affected dogs, while the concentrations of α2-and β1-globulins were significantly higher (P=0.0032 and P=0.0021, respectively) compared to dogs without mammary gland tumors. In the concentrations of acute phase proteins, significantly higher mean concentrations of C-reactive protein and haptoglobin were obtained in dogs with mammary tumors (P=0.0025 and P=0.0002, respectively). The values of α1-acid glycoprotein did not vary markedly between the bitches with and without mammary tumors. Presented data suggest that neoplastic processes in the mammary gland may also alter the electrophoretic pattern of blood serum proteins and induce changes in the production of some inflammatory proteins. [ABSTRACT FROM AUTHOR]
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- 2023
- Full Text
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33. Methods of Formation of Protective Inhibited Polymer Films on Tungsten.
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Shapagina, Natalia A., Shapagin, Alexey V., Dushik, Vladimir V., Shaporenkov, Andrey A., Nikulova, Uliana V., Stepanenko, Valentina Yu., Matveev, Vladimir V., Klyuev, Alexey L., and Loginov, Boris A.
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POLYMER films , *TUNGSTEN , *TUNGSTEN trioxide , *ELECTROPHORESIS , *AQUEOUS solutions - Abstract
A comparative study of anticorrosive inhibited polymer films on the tungsten surface formed from an aqueous solution of inhibited formulations (INFOR) containing organosilane and corrosion inhibitors was carried out by means of the prolonged exposure of a tungsten product in a modifying solution and by the method of cataphoretic deposition (CPD). Depending on the method of forming films on tungsten, the molecular organization of the near-surface layers was studied (ATR-FTIR), and the subprimary structure of the films was explored (TEM). The optimal modes of cataphoresis deposition (CPD duration and current density applied to the sample) for the formation of a protective inhibited polymer film on the tungsten surface were established by means of SEM. The energy and thermochemical characteristics (sessile drop and DSC methods), as well as operational (adhesive behavior) and protective filming ability (EIS and corrosion behavior), according to the method of formation of inhibited polymer film, were determined. Based on the combined characteristics of the films obtained by the two methods and the deposition modes, the CPD method showed better performance than the electroless dipping method. [ABSTRACT FROM AUTHOR]
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- 2023
- Full Text
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34. The effect of a diet based on semifinished products from plant and animal raw materials on reproductive capacity, growth, and development of the organism.
- Author
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Tkach, Gennadii, Omelian, Alina, Kushnir, Yurii, and Altanova, Olena
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RAW materials , *PLANT reproduction , *ANIMAL reproduction , *PROTEINS , *FOOD consumption - Abstract
The demand among the population for protein products, which are essential for a healthy diet, is a pressing issue today, which constantly requires scientists and manufacturers to search for alternative protein analogues. That is why the aim of the research was to study the impact of a diet based on plant and animal raw materials on the reproductive capacity, growth, and development of the body. In the experiment, the duration of pregnancy in pregnant females was determined; the average number of rats born per female of the respective group; the weight of females before pregnancy; the weight of females on the 7th, 14th and 21st day of pregnancy. The postnatal development of rats of the control and experimental groups was compared. In newborn rats, the following were determined: age of ear drum detachment; craniocaudal size at birth; craniocaudal size on the 5th day; age of hair coat appearance; age of eye-opening; weight of rats at birth; weight of rats on the 7th, 14th and 30th day of life. The results obtained indicate that there is no negative effect of the vegetable semifinished product using soya-wheat texture and meat cutlet on the reproductive functions of rats and the development of offspring. During the experiment, female rats showed no signs of toxicosis, visible pregnancy disorders, or physiological disorders. During the dynamic observation of the weight of experimental females in all experimental groups, no decrease in weight gain was recorded compared to control animals. The weight and postnatal dimensions of newborn rats of the experimental groups did not differ from those of the control. For the first time in laboratory animals, based on an integrated approach using modern research methods, the effect of long-term consumption of food based on semifinished products from soy-wheat texture and meat cutlets on the reproductive functions of rats and the development of offspring was studied, which is of practical value in the context of the health of females and offspring of mammals under the influence of an alternative nutritional factor. [ABSTRACT FROM AUTHOR]
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- 2023
- Full Text
- View/download PDF
35. Seminal Plasma Proteins Associated with The Fertility of Brahman Bulls in The Colombian Low Tropics.
- Author
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Chacón, Liliana J., Yepes, Germán D., Cardozo, Jaime, Rueda, Fabian, Castillo, Viviana, Torres, Andrés, Martins, Jorge, and Ardila, Ariosto
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SEMINAL proteins , *POLYACRYLAMIDE gel electrophoresis , *FERTILITY , *GENITALIA , *CATTLE fertility , *TROPICAL conditions - Abstract
The sperm interacts with seminal plasma proteins during its transport through the female reproductive tract to reach the oocyte. Seminal plasma proteins have been associated as biomarkers of fertility in bovine males, while two-dimensional electrophoresis in polyacrylamide gels under denaturing conditions (2D-PAGE) is a useful technique for their separation, allowing their subsequent analysis with the aid of specialised software. Brahman bulls are known for their tolerance to tropical conditions such as low-quality pastures, high temperatures, and relative humidity as well as moderate resistance to infestations by parasites and insects. The present study describes the two-dimensional electrophoretic profiles of the seminal plasma proteins in the rainy and dry seasons, associating them with the fertility of Brahman bulls in the Colombian Orinoquía in a 90-days breeding season and a single-sire mating system (1 bull per 50 Brahman cows) with 60 consecutive days of rest. The fertility-related seminal plasma protein spots increased in the dry season. Likewise, a meaningful relationship was found between the protein spots that possibly coincide with the Binder of Sperm Proteins. It was also found that bulls with the highest percentages of pregnancy also had similarities in their 2D seminal plasma maps. We conclude that the seminal plasma protein profile of Brahman bulls raised in the Colombian low tropic changes between rainy and dry seasons, and such changes may influence the reproductive performance of those animals. [ABSTRACT FROM AUTHOR]
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- 2023
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36. Overview of Capillary Electrophoresis Analysis of Alkaline Phosphatase (ALP) with Emphasis on Post-Translational Modifications (PTMs).
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Balbaied, Thanih and Moore, Eric
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POST-translational modification , *CAPILLARY electrophoresis , *ISOENZYMES , *ALKALINE phosphatase , *ELECTROPHORESIS , *ENZYMES - Abstract
Alkaline phosphatase is a vital enzyme used in separation studies and as a biomarker for liver, bone, and certain cancer conditions. Its stability and specific properties enable insights into enzyme behavior, aiding in the development of detection methods with broader applications in various scientific fields. Alkaline phosphatase has four main isoenzymes: GCAP, IAP, PLAP, and TNAP, each with distinct roles. TNAP is found in the liver, kidney, and bones, playing a role in bone mineralization. The functions of the other isoenzymes are not fully known. Separation techniques like electrophoresis and chromatography are valuable for studying enzymes and proteins, revealing insights into their structure and function in pharmaceutical research and PTM studies. The main goal of this review paper is to thoroughly evaluate how capillary electrophoresis is applied to analyze alkaline phosphatase. It seeks to investigate the latest advancements in capillary electrophoresis and how they can improve the sensitivity, selectivity, and efficiency of alkaline phosphatase analysis. [ABSTRACT FROM AUTHOR]
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- 2023
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37. In Silico and Validation Approaches for Optimum Conditions of Rattus norvegicus Target Gene qPCR Primers.
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Pollo, Gracia Alice Victoria, Antara, Nyoman Yudi, Alamsyah, Firman, and Pratiwi, Rarastoeti
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RATTUS norvegicus , *POLYMERASE chain reaction , *DNA primers , *ELECTROPHORESIS , *FIBROBLASTS - Abstract
Gene expression analysis using the qPCR method requires an oligonucleotide pair to prime the amplification process. Supporting the widely used qPCR method, variety of qPCR reagents and primer options are available. This infers to the importance of in silico and laboratory experimental validation approach considering SYBR Green optimum annealing temperature to validate the most suitable primer for prior use. Several genes are suspected to be involved in the BM-MSCs migration and differentiation into Cancer-associated Fibroblast Cells in Rattus norvegicus. This article aims to provide in silico analysis with the case of the suspected genes namely actin alpha-2 smooth muscle (ACTA2), fibroblast activation protein (FAP), hypoxanthine phosphoribosyltransferase-1 (HPRT1), platelet-derived growth factor subunit B (PDGFB), phosphoinositide-3-kinase regulatory subunit-1 (PIK3R1), and vascular cell adhesion molecule-1 (VCAM1) qPCR primer with qPCR and electrophoresis validation. The procedure used in this approach was in silico analysis of primer from published articles and newly designed primer. The analysis was done with Primer-BLAST for gene specificity, Primer-Dimer, OligoCalc for hairpin formation, BLAST Nucleotide for identical sequence screening, and Clustal Omega for product length validation. Experimental validation was done using qPCR for optimal annealing temperature, priming ability, amplicon specificity, and electrophoresis for product length validation. This assessment resulted in in silico and laboratory experimental validation of ACTA2, FAP, HPRT1, PDGFB, PIK3R1, and VCAM1 primer pairs producing suitable amplicon for qPCR using Rattus norvegicus cDNA with SYBR annealing temperature range of 60-65° C with three mM MgCl2. The primer pairs can be used for further qPCR analysis under similar conditions and the procedure stated can be used as starting point for qPCR Primer preparation accounting for fluorophore optimum annealing temperature. [ABSTRACT FROM AUTHOR]
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- 2023
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38. Evaluation of Systemic Genotoxic/Oxidative and Proinflammatory Effects in Workers of a Titanium Dioxide Production Plant.
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Cavallo, Delia, Fresegna, Anna Maria, Ciervo, Aureliano, Maiello, Raffaele, Chiarella, Pieranna, Buresti, Giuliana, Del Frate, Valentina, Di Basilio, Marco, Iavicoli, Sergio, and Ursini, Cinzia Lucia
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INTERLEUKINS , *KRUSKAL-Wallis Test , *ELECTROPHORESIS , *ONE-way analysis of variance , *MULTIPLE regression analysis , *OCCUPATIONAL exposure , *FISHER exact test , *MANN Whitney U Test , *OXIDATIVE stress , *T-test (Statistics) , *AGAR , *TOXICITY testing , *DNA-binding proteins , *CHI-squared test , *DESCRIPTIVE statistics , *DNA damage , *TITANIUM , *GENETIC techniques , *DATA analysis software - Abstract
This study is aimed at evaluating whether the occupational exposure to TiO2 during the industrial production process is able to induce genotoxic, oxidative, and inflammatory effects on blood, biomonitoring the same workers that showed micronucleus induction in the exfoliated buccal cells, as previous published. The final aim was to find sensitive and suitable biomarkers to evaluate potential early toxicity of occupational exposure to TiO2. On the same 40 workers involved in the manufacture of TiO2 pigment, 5 office workers, and 18 controls previously studied, we used formamidopyrimidine glycosylase- (Fpg-) comet assay on lymphocytes to evaluate genotoxic/oxidative effects and detected cytokine (IL-6, IL-8, and TNFα) release by ELISA to evaluate proinflammation. Moreover, we studied the possible influence of single nucleotide polymorphisms of XRCC1 and hOGG1 DNA repair genes and of GST metabolism-related genes (GSTT1 and GSTM1) on the evaluated effects. We did not find statistically significant differences in the mean values of the analysed Fpg-comet assay parameters; only the percentage of DNA damaged cells appearing in the test as comets (% comets) resulted higher in the exposed workers compared to controls. Also, the data analysed taking into account the specific task (bagging, industrial cleaning, mobile operations, maintaining, and production) showed differences only for % comets which resulted higher in industrial cleaners compared to controls. We found variations of IL-6 and IL-8 levels in the exposed workers with concentrations that were lower for IL-6 and higher for IL-8 compared to the control group. XRCC1, hOGG1, and GSTT1 polymorphisms did not influence neither comet parameters nor cytokine release. These findings demonstrate that TiO2 production process is able to induce slight proinflammatory effects in terms of IL-8 increased release but not significant genotoxic/oxidative effects on lymphocytes, which do not seem to be a target of TiO2, prevalently inhalable particles, generated in the studied production site. [ABSTRACT FROM AUTHOR]
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- 2023
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39. Pre-Referral Primary Care Blood Tests and Symptom Presentation before Cancer Diagnosis: National Cancer Diagnosis Audit Data.
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Cranfield, Ben M., Abel, Gary A., Swann, Ruth, Moore, Sarah F., McPhail, Sean, Rubin, Greg P., and Lyratzopoulos, Georgios
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TUMOR diagnosis , *AUDITING , *LIVER function tests , *UREA , *PREDICTIVE tests , *TIME , *INFLAMMATION , *ELECTROPHORESIS , *FERRITIN , *PRIMARY health care , *BENCHMARKING (Management) , *AMYLASES , *MEDICAL referrals , *MEDICAL records , *DESCRIPTIVE statistics , *RESEARCH funding , *BLOOD testing , *TUMORS , *BLOOD cell count , *ELECTROLYTES , *TUMOR markers , *SYMPTOMS - Abstract
Simple Summary: Blood tests can support decisions by GPs about referring patients who present with symptoms of possible cancer for specialist assessment. This study analysed data on the use of blood tests in primary care in patients subsequently diagnosed with cancer to understand how often and when blood tests were used. We found that the use of generic blood tests (including full blood count, urea and electrolyte, liver function, and inflammatory marker tests) varied widely between patients presenting with different symptoms, with greater use in patients presenting with certain nonspecific symptoms (e.g., fatigue or loss of weight) and least frequently in those presenting with certain red-flag symptoms (e.g., breast or skin symptoms). Blood tests with greater specificity to certain organs/pathologies (including serum protein electrophoresis, ferritin, bone profile, and amylase tests) followed a similar use pattern regarding symptom specificity but at a lower use frequency. Commonly used cancer biomarkers were used in varying proportions depending on whether the presenting symptom could be related to prostate or ovarian cancer (for example, 88% of men presenting with lower urinary tract symptoms had presumed PSA measurement). The findings benchmark how often blood tests are used in certain clinical scenarios and identify opportunities for greater use in patients with symptoms of low (<3%) positive predictive value for cancer. Background: Blood tests can support the diagnostic process in primary care. Understanding how symptomatic presentations are associated with blood test use in patients subsequently diagnosed with cancer can help to benchmark current practices and guide interventions. Methods: English National Cancer Diagnosis Audit data on 39,751 patients with incident cancer in 2018 were analysed. The frequency of four generic (full blood count, urea and electrolytes, liver function tests, and inflammatory markers) and five organ-specific (cancer biomarkers (PSA or CA125), serum protein electrophoresis, ferritin, bone profile, and amylase) blood tests was described for a total of 83 presenting symptoms. The adjusted analysis explored variation in blood test use by the symptom-positive predictive value (PPV) group. Results: There was a large variation in generic blood test use by presenting symptoms, being higher in patients subsequently diagnosed with cancer who presented with nonspecific symptoms (e.g., fatigue 81% or loss of appetite 79%), and lower in those who presented with alarm symptoms (e.g., breast lump 3% or skin lesion 1%). Serum protein electrophoresis (reflecting suspicion of multiple myeloma) was most frequently used in cancer patients who presented with back pain (18%), and amylase measurement (reflecting suspicion of pancreatic cancer) was used in those who presented with upper abdominal pain (14%). Prostate-specific antigen (PSA) use was greatest in men with cancer who presented with lower urinary tract symptoms (88%), and CA125 in women with cancer who presented with abdominal distention (53%). Symptoms with PPV values between 2.00–2.99% were associated with greater test use (64%) compared with 52% and 51% in symptoms with PPVs in the 0.01–0.99 or 1.00–1.99% range and compared with 42% and 31% in symptoms with PPVs in either the 3.00–4.99 or ≥5% range (p < 0.001). Conclusions: Generic blood test use reflects the PPV of presenting symptoms, and the use of organ-specific tests is greater in patients with symptomatic presentations with known associations with certain cancer sites. There are opportunities for greater blood test use in patients presenting with symptoms that do not meet referral thresholds (i.e., <3% PPV for cancer) where information gain to support referral decisions is likely greatest. The findings benchmark blood test use in cancer patients, highlighting opportunities for increasing use. [ABSTRACT FROM AUTHOR]
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- 2023
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40. Application aspects of joint anaphoresis/substrate anodization in production of biocompatible ceramic coatings.
- Author
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BOŽIĆ, KATARINA Đ., PAVLOVIĆ, MIROSLAV M., ŠEKULARAC, GAVRILO M., PANIĆ, STEFAN V., and PAVLOVIĆ, MARIJANA R. PANTOVIĆ
- Subjects
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ELECTROPHORETIC deposition , *ELECTROPHORESIS , *PROTECTIVE coatings , *ANODIC oxidation of metals , *CERAMIC coating , *Z bosons , *CORROSION resistance , *METALLIC surfaces - Abstract
Electrophoretic deposition (EPD) occurs as a cataphoretic deposition - the coating is deposited on the cathode, and anaphoretic deposition - the coating is deposited on the anode. The primary purpose of EPD is to obtain compact and uniform organic/inorganic coatings of the desired thickness and adhesion on metal surfaces by applying an electric field to the particles of coating precursor. EPD basic principles for coatings deposition concerning fundamental explanations and considerations of practical parameters of the process are presented. Cataphoretic deposition has become popular because it can apply organic coatings to complex structures that are otherwise very difficult to coat. These coatings were found to improve the characteristics of the substrate, such as biocompatibility, appearance and resistance to the corrosion processes. The key EPD parameters are composition, pH value and viscosity of deposition medium, as well as zeta potential of the particles, electric field strength, etc. A special survey is given to the process of anaphoretic deposition, which is relatively new, and its advantages over cataphoretic deposition are discussed. Through the process of joint anaphoresis/substrate anodization process, the surface of the substrate is simultaneously anodized and modified by incorporation of the foreign particles into the anodic layer. The coatings of mixed composition of better adhesion and corrosion resistance with respect to cataphoretically-deposited coatings are obtained as result. [ABSTRACT FROM AUTHOR]
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- 2023
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41. Systematic Review on Copper Calcium Hydroxide Nanoparticles in the Treatment of Apical Periodontitis.
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R., Marlin Lina, Sindhu R., Bharathwaj V. V., Dhamodhar, Dinesh, Sathiyapriya S., Prabu D., and Rajmohan M.
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PERIAPICAL periodontitis , *CALCIUM hydroxide , *ROOT canal treatment , *COPPER , *NANOPARTICLES , *DENTAL pulp cavities - Abstract
Apical periodontitis is an inflammation and destruction of the periradicular tissues due to various insults to the pulp in the form of infection, trauma, and faulty dental procedures. Conditions regarding this are treated employing root canal therapy. Recent innovations show that copper calcium hydroxide nanoparticles and their electrophoresis can be used to treat apical periodontitis effectively. To evaluate the effectiveness of copper calcium hydroxide nanoparticles in the treatment of apical periodontitis, a literature search was done using PubMed, Cochrane Central Register of controlled Trials (CENTRAL), Science Direct, and Lilacs using the MeSH (Medical Subject Heading) terms Copper calcium hydroxide, apical periodontitis, nanoparticles, and electrophoresis. Of a total of 167 articles screened, 44 were full-text articles assessed for eligibility and four articles were taken for the qualitative analysis. This review was recorded according to the PRISMA GUIDELINES. Four randomized controlled trials were included in the review process. The copper calcium hydroxide particles were compared with other root canal medicament materials. It was found that copper calcium hydroxide and its electrophoresis were used in the treatment of apical periodontitis and were found to be one of the effective methods. Copper calcium hydroxide nanoparticles were effective against the destruction of the periradicular tissues. [ABSTRACT FROM AUTHOR]
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- 2023
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42. Small Molecule RBI2 Disrupts Ribosome Biogenesis through Pre-rRNA Depletion.
- Author
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Scull, Catherine E., Twa, Guy, Zhang, Yinfeng, Yang, Naiheng J., Hunter III, Robert N., Augelli-Szafran, Corinne E., and Schneider, David A.
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RNA analysis , *SMALL molecules , *IN vitro studies , *REVERSE transcriptase polymerase chain reaction , *NUCLEOTIDE separation , *SEQUENCE analysis , *MELANOMA , *ELECTROPHORESIS , *RNA , *PRECIPITIN tests , *CELL survival , *IMMUNOBLOTTING , *CELL proliferation , *GENE expression profiling , *AGAR , *RESEARCH funding , *CELL lines , *ANALYTICAL chemistry techniques , *DATA analysis software , *BREAST tumors - Abstract
Simple Summary: Ribosome biosynthesis has emerged as an excellent target for inhibition of cancer cell growth and proliferation. We used a high throughput screen to identify potent inhibitors of ribosome synthesis in malignant melanoma cells. Here, we show that the newly identified ribosome biogenesis inhibitor, RBI2, inhibits multiple cancer cell types by a mechanism of action that is distinct from previously described compounds. Using chromatin IP, isotopic labelling, and RNA sequencing, we conclude that treatment with RBI2 does not inhibit transcription initiation by RNA polymerase I, but appears to induce rapid polyadenylation and degradation of ribosomal RNA. This mechanism of action would be unique from previously described inhibitors of RNA polymerase I. Cancer cells are especially sensitive to perturbations in ribosome biogenesis as they rely on finely tuned protein homeostasis to facilitate their rapid growth and proliferation. While ribosome synthesis and cancer have a well-established relationship, ribosome biogenesis has only recently drawn interest as a cancer therapeutic target. In this study, we exploited the relationship between ribosome biogenesis and cancer cell proliferation by using a potent ribosome biogenesis inhibitor, RBI2 (Ribosome Biogenesis Inhibitor 2), to perturb cancer cell growth and viability. We demonstrate herein that RBI2 significantly decreases cell viability in malignant melanoma cells and breast cancer cell lines. Treatment with RBI2 dramatically and rapidly decreased ribosomal RNA (rRNA) synthesis, without affecting the occupancy of RNA polymerase I (Pol I) on the ribosomal DNA template. Next-generation RNA sequencing (RNA-seq) revealed that RBI2 and previously described ribosome biogenesis inhibitor CX-5461 induce distinct changes in the transcriptome. An investigation of the content of the pre-rRNAs through RT-qPCR revealed an increase in the polyadenylation of cellular rRNA after treatment with RBI2, constituting a known pathway by which rRNA degradation occurs. Northern blotting revealed that RBI2 does not appear to impair or alter rRNA processing. Collectively, these data suggest that RBI2 inhibits rRNA synthesis differently from other previously described ribosome biogenesis inhibitors, potentially acting through a novel pathway that upregulates the turnover of premature rRNAs. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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43. A novel stop codon mutation in STK11 gene is associated with Peutz-Jeghers Syndrome and elevated cancer risk: a case study.
- Author
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Khanabadi, Binazir, Seyfi, Diba Najafgholizadeh, Rejali, Leili, Taleghani, Mohammad Yaghoob, Tavallaei, Mehdi, Shahrokh, Shabnam, Abkenar, Elahe Daskar, Noukabadi, Fatemeh Naderi, Aghdaei, Hamid Asadzadeh, and Mojarad, Ehsan Nazemalhosseini
- Subjects
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BIOMARKERS , *STOMACH , *GENETIC mutation , *SEQUENCE analysis , *COLONOSCOPY , *COLON (Anatomy) , *STAINS & staining (Microscopy) , *INTESTINAL polyps , *ELECTROPHORESIS , *BLOOD collection , *COLORECTAL cancer , *TRYPTOPHAN , *TUMOR suppressor genes , *HAMARTOMA , *AGAR , *PEUTZ-Jeghers syndrome , *ABDOMINAL pain , *COMPUTED tomography , *ENDOSCOPIC gastrointestinal surgery , *POLYMERASE chain reaction , *TYROSINE , *FAMILY history (Medicine) , *DISEASE risk factors - Abstract
Based on the analysis of patients with Peutz-Jeghers syndrome (PJS), Serine threonine kinase11 (STK11) is known as a tumor suppressor gene, which is involved in cell polarization, regulation of apoptosis, and DNA damage response. In this case report study, we examined STK11 gene sequencing in a 42-year-old woman with mucocuta neous pigmentation and positive family history. Endoscopy and colonoscopy showed >1000 polyps throughout the stomach/colon (PJ-type hamartomas). The larger polyp in the stomach was resected and the small bowel imaging detected multiple jejunum/ileum small polyps. The data released from the sequencing results revealed five alterations in exons 1 to 5. The major mutation in stop codon was reported as converted to the amino acid tryptophan (TRP) to tyrosine (TER). The TGG codon was converted to TAG by mutation. Finally, another novel mutation in STK11 stop codon as a 'de novo' variant was seen. It is predicted that stop codon mutations make the affected person susceptible to developing colorectal cancer. [ABSTRACT FROM AUTHOR]
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- 2023
- Full Text
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44. Spectrum of Thalassemia and Hemoglobinopathy Using Capillary Zone Electrophoresis: A Facility-Based Single Centred Study at icddr,b in Bangladesh.
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Hasan, Anamul, Ahmed, Jigishu, Chanda, Bikash Chandra, Aniqua, Maisha, Akther, Raisa, Dhar, Palash Kanti, Hasan, Kazi Afrin Binta, Siddique, Abdur Rouf, Islam, Md. Zahidul, Urmee, Sharmine Zaman, and Mondal, Dinesh
- Subjects
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CAPILLARY electrophoresis , *HEMOGLOBINOPATHY , *BLOOD cell count , *THALASSEMIA , *FETAL hemoglobin , *HYDROPS fetalis - Abstract
Background: Although the global thalassemia zone covers Bangladesh, there are very limited studies conducted in this region. Therefore, the focus of our study is to understand the prevalence and burden of thalassemia and hemoglobinopathy in Bangladesh. Methods: The analysis was based on a retrospective evaluation of laboratory diagnoses between 2007 January and 2021 October. A total of 8503 specimens were sampled and analyzed which were either referred by corresponding physicians or self-referred. This was neither any epidemiological nationwide survey nor was the study population chosen randomly. Hematological data were obtained through capillary zone electrophoresis and corresponding complete blood count. Results: 1971 samples (~23.18% of the total) were found with at least one inherited hemoglobin disorder. The most common hemoglobin disorder observed was the hemoglobin E (Hb E) trait (10.67%), followed by the β-thalassemia trait (8.4%), homozygotic Hb E (1.59%), and Hb E/β-thalassemia (1.58%). Other variants found in this study with minimal percentages were Hb N-Seattle, Hb S, Hb D-Punjab, Hb Lepore, Hb C, Hb Hope, Hb H, and hereditary persistence of fetal hemoglobin. Discussion: The pattern of thalassemia and hemoglobinopathy in our study is diverse and heterogeneous. A broad and detailed spectrum of such inherited hemoglobin disorders will ultimately be helpful in implementing nationwide thalassemia management and strategy policy in Bangladesh. [ABSTRACT FROM AUTHOR]
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- 2023
- Full Text
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45. Characterization of the Components and Functional Properties of a Plasma-Derived Fibrin Sealant.
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Ramaiola, Ilaria, Lopez, Laura, Moron, Manuel, Maduell, Pau, López, Maite, Marzo, Nuria, and Grancha, Salvador
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FIBRIN tissue adhesive , *BIOCHEMISTRY , *PROTHROMBIN time , *FIBRINOLYTIC agents , *BLOOD proteins , *PHENOMENOLOGICAL biology , *ELECTROPHORESIS , *WESTERN immunoblotting , *MICROSCOPY , *HEMOSTASIS , *RESEARCH funding , *FIBRINOGEN , *DYES & dyeing , *TENSILE strength , *BLOOD coagulation factors , *VISCOSITY , *THROMBIN , *SPECTROPHOTOMETRY - Abstract
Background: The activity and purity of Fibrin Sealant Grifols are described in this article. This fibrin sealant contains two components (fibrinogen and thrombin) that mimic the final stages of clotting. Fibrin sealants are primarily used as an adjunct to hemostasis for mild to moderate surgical bleeding in adults when standard surgical techniques are ineffective or impractical. Objective: The aim of these studies was to assess the biochemical and functional characteristics of Fibrin Sealant Grifols. Methods: Total protein, plasma protein impurities, and molecular forms were analyzed for both components. Clottable fibrinogen and thrombin activity were also measured. Both components were analyzed by SDS-PAGE and Western blot. The fibrin sealant clot was functionally characterized. Results: The components had minimal contamination by other plasma proteins and no aggregates or degradation products. The fibrinogen component was almost completely clottable fibrinogen. The thrombin component contains albumin. The fibrinogen component formed a fibrin polymer and clots with tensile strength similar to literature values. The application device applied the components in a 1:1 volume ratio. Clotting time was ∼ 5 s when applied with the drip tip and nearly instantaneous when applied with the airless spray apparatus. Conclusion: This fibrin sealant was shown to contain highly purified fibrinogen and thrombin delivered in a 1:1 volume ratio. These properties contribute to the clinical safety and efficacy of this product for many applications. [ABSTRACT FROM AUTHOR]
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- 2023
- Full Text
- View/download PDF
46. Work-up of Patients with Decreased Hemoglobin A2 Identified by Capillary Zone Electrophoresis: A North American Institutional Experience.
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Moore, Margaret and Courville, Elizabeth L
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ANEMIA diagnosis , *CLINICAL pathology , *REFERENCE values , *HEMOGLOBINS , *IRON , *HYPOCHROMIC anemia , *FERRITIN , *IRON in the body , *RETROSPECTIVE studies , *ACQUISITION of data , *FISHER exact test , *GENETIC testing , *CAPILLARY electrophoresis , *PEARSON correlation (Statistics) , *T-test (Statistics) , *IRON deficiency , *MEDICAL records , *RADIATION doses , *DESCRIPTIVE statistics , *CHI-squared test , *PLATELET count , *DATA analysis software , *BLOOD cell count , *ERYTHROCYTES - Abstract
Objective Isolated low hemoglobin A2 (HbA2) is rarely encountered in our clinical practice using capillary zone electrophoresis. The study goal was to characterize the work-up at our institution of patients with low HbA2. Methods Patients with low HbA2 and a control cohort with normal capillary zone electrophoresis were identified and relevant information extracted from the medical record. Results Of 44 patients with isolated decreased HbA2, 28 (64%) had corresponding complete blood count/ferritin values. Compared to control patients, patients with low HbA2 were more likely to have iron deficiency and demonstrated a more microcytic, hypochromic blood picture. However, 46% (13/28) of patients with low HbA2 and ferritin for evaluation did not have iron deficiency. Only 2 patients had genetic testing. Conclusion This study redemonstrates the association between low HbA2 and iron deficiency and reinforces the need for iron indices to interpret capillary zone electrophoresis results. Our study population showed incomplete or absent iron studies in most cases. [ABSTRACT FROM AUTHOR]
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- 2023
- Full Text
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47. Establishment and Evaluation of Recombinant Expression of HCV Transmembrane Protein (p7) and Detection of Anti-p7 Antibody in Serum of HCV-Infected Patients by Chemiluminescence.
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Zhou, Huajun, Wu, Jie, Yu, Yu, Dai, Yuzhu, Jin, Xiaojuan, Sun, Qingyang, Che, Feihu, Zhang, Yingjie, and Cheng, Jun
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IMMUNOGLOBULINS , *CHRONIC hepatitis C , *CHEMILUMINESCENCE assay , *ELECTROPHORESIS , *HEPATITIS C , *ANTIVIRAL agents , *PLASMIDS , *GENE expression , *MEMBRANE transport proteins , *RESEARCH funding , *ENZYME-linked immunosorbent assay , *GENOTYPES , *POLYMERASE chain reaction , *RECOMBINANT proteins , *NUCLEIC acids - Abstract
Objective Our aim was to establish a chemiluminescence method for detecting anti-transmembrane protein (p7) antibody in the serum of patients with hepatitis C virus (HCV) infection. Methods The p7 gene was amplified by polymerase chain reaction using the plasmid PUC-p7 containing the p7 nucleic acid sequence of the HCV 1b genotype as the template, and recombinant plasmid pGEX-KG-p7 was constructed. After p7 fusion, the protein was induced and expressed in the prokaryote, extracted, and purified; the anti-p7 antibody detection kit was prepared, and its efficacy was evaluated. Results The plasmid pGEX-KG-p7 was constructed correctly, and p7 fusion protein was obtained. The methodological indexes of the kit, the precision test, blank limit and detection limit, etc, met the requirements. The positive rate of serum anti-p7 antibody in 45 patients with HCV infection was 20%. Conclusions The kit can be used in screening diagnosis, condition monitoring, prognosis, and disease mechanism and epidemiological study of HCV infection. The p7 protein has immune response in HCV-infected patients. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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48. Diffusiophoresis of a Weakly Charged Liquid Metal Droplet.
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Fan, Leia, Lin, Jason, Yu, Annie, Chang, Kevin, Tseng, Jessica, Su, Judy, Chang, Amy, Lu, Shirley, and Lee, Eric
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LIQUID metals , *ELECTROLYTE solutions , *DROPLETS , *NANOMEDICINE , *LIPOSOMES , *GALLIUM - Abstract
Diffusiophoresis of a weakly charged liquid metal droplet (LMD) is investigated theoretically, motivated by its potential application in drug delivery. A general analytical formula valid for weakly charged condition is adopted to explore the droplet phoretic behavior. We determined that a liquid metal droplet, which is a special category of the conducting droplet in general, always moves up along the chemical gradient in sole chemiphoresis, contrary to a dielectric droplet where the droplet tends to move down the chemical gradient most of the time. This suggests a therapeutic nanomedicine such as a gallium LMD is inherently superior to a corresponding dielectric liposome droplet in drug delivery in terms of self-guiding to its desired destination. The droplet moving direction can still be manipulated via the polarity dependence; however, there should be an induced diffusion potential present in the electrolyte solution under consideration, which spontaneously generates an extra electrophoresis component. Moreover, the smaller the conducting liquid metal droplet is, the faster it moves in general, which means a smaller LMD nanomedicine is preferred. These findings demonstrate the superior features of an LMD nanomedicine in drug delivery. [ABSTRACT FROM AUTHOR]
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- 2023
- Full Text
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49. Molecular Diagnosis and Phylogenetic Investigation of Entamoeba histolytica and Giardia intestinalis among Diarrheal Patients in Iraq.
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Al-Rawi, Ola Nasrat A. and Al-Omer, Najah Subhi N.
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DNA analysis , *FECAL analysis , *MOLECULAR diagnosis , *DIARRHEA , *PHYLOGENY , *CROSS-sectional method , *MICROSCOPY , *ELECTROPHORESIS , *PROTOZOAN diseases , *DESCRIPTIVE statistics , *GENE expression profiling , *GIARDIA lamblia , *POLYMERASE chain reaction , *NUCLEIC acid amplification techniques - Abstract
Background and Aim: Entamoeba histolytica and Giardia intestinalis are two common parasites associated with gastrointestinal infections and diarrheal diseases worldwide. This study investigated the prevalence, molecular characterization, and phylogenetic relationships of E. histolytica and G. intestinalis among patients with diarrhea and intestinal disorders in hospitals across Iraq. Materials and Methods: Stool samples were collected from individuals presenting with gastrointestinal symptoms, and a microscopic examination was performed to screen for the presence of E. histolytica and G. intestinalis. DNA was extracted from positive samples, and PCR amplification targeting the 16S rRNA gene for E. histolytica and the triose phosphate isomerase (TPI) gene for G. intestinalis was carried out. The resulting PCR products were subjected to agarose gel electrophoresis, purified, and sequenced. Phylogenetic trees were constructed using the Maximum Likelihood method based on the Tamura-Nei model, and bootstrap analysis was performed. Results: Among the studied samples, 27.8% (n=85) were positive for E. histolytica, while 19.6% (n=60) were positive for G. intestinalis. Sequence analysis confirmed the presence of E. histolytica and G. intestinalis by matching the obtained sequences with those available in the NCBI gene bank. The phylogenetic analysis revealed the genetic diversity and relatedness of the E. histolytica and G. intestinalis isolates. Clustering patterns specific to the Iraqi population were observed, suggesting potential regional transmission dynamics. Conclusion: This study provides valuable insights into the prevalence, molecular characterization, and phylogenetic relationships of E. histolytica and G. intestinalis in Iraqi patients with diarrhea and intestinal disorders. [ABSTRACT FROM AUTHOR]
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- 2023
- Full Text
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50. Metabolic Parameters and Oral Microbiota in Patients with Atherosclerosis.
- Author
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Nazari, Zahra, Abiri, Ramin, Moghadam, Reza Heidary, Chehri, Goodarz, Alvandi, Amirhooshang, and Mohajerani, Hamid Reza
- Subjects
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ORAL microbiology , *SALIVA analysis , *BIOMARKERS , *CARDIOVASCULAR diseases risk factors , *BLOOD urea nitrogen , *DNA , *ORAL health , *ELECTROPHORESIS , *CASE-control method , *LOW density lipoproteins , *BLOOD sugar , *ATHEROSCLEROSIS , *T-test (Statistics) , *HUMAN microbiota , *POLYMERASE chain reaction , *LOGISTIC regression analysis , *CHOLESTEROL - Abstract
Background and Aim: Oral infections are common among people of any age and can trigger systemic inflammation. The microbiota is a diverse group of microorganisms that play important roles in metabolism, immune function, and homeostasis. Oral microbiota in human atherosclerotic plaques has been identified using various techniques. Therefore, the focus of this study was to determine the correlation between metabolic parameters and oral microbiota composition in patients with atherosclerosis using Denaturing Gradient Gel Electrophoresis (DGGE) assays. Materials and Methods: In this case-control study, saliva samples were collected from 139 patients with atherosclerosis and healthy individuals from Imam Ali Cardiovascular Hospital, Kermanshah, Iran. After DNA extraction, PCR products were examined and evaluated using DGGE assays. Results: The study included 89 (36%) patients with a history of atherosclerosis and 50 (36%) healthy individuals. There was a significant relationship between the mean total cholesterol, Low-Density Lipoprotein (LDL), Fasting Blood Sugar (FBS), and Blood Urea Nitrogen (BUN) in the two groups. However, there was no significant difference in the mean high-density lipoprotein (HDL) and Triglyceride levels between the study groups. Conclusion: Our results showed a relationship between metabolic parameters and oral microbiota composition in patients with atherosclerosis. Additionally, our results indicated that the DGGE assay is a useful method for diagnosing and comparing the oral microbiota of people with atherosclerosis and healthy individuals. Therefore, further examination of the oral microbiota is necessary to determine its potential as a biomarker for atherosclerosis. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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