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17 results on '"E. Belau"'

1. $$\bar K^{0*} (892)$$ andK 0*(892) production at lowp t and the quark parton model

Author

H. J. Seebrunner, C.J.S. Damerell, H. Palka, Z. Hajduk, V. Castillo, H.G.J.M. Tiecke, R. J. Tapper, G. Lütjens, S. Watts, T. Gooch, B.D. Hyams, V. Chabaud, G. de Rijk, H.F. Fawcett, M. Turala, S. Kwan, E. Higon, A. R. Gillman, H. Becker, J. Kemmer, B. Lücking, D. P. Kelsey, E. Belau, U. Kötz, A. Wylie, T. Zeludziewicz, D. Bucholz, J. Richardson, C. Daum, M. Bosman, H. Dietl, G. Waltermann, M. Pepé, J. Malos, W. Männer, S. Gill, M. Rozanska, P. Weilhammer, L. W. Wiggers, G. Polok, Fred Wickens, K. Rybicki, T. Böhringer, E. Neugebauer, G. Lutz, R.S. Gilmore, U. Stierlin, P. Gras, S. Barlag, and R. Klanner

Subjects
Quark, Physics, Particle physics, Physics and Astronomy (miscellaneous), Bar (music), Nuclear Theory, High Energy Physics::Phenomenology, Hadron, Parton, Elementary particle, Particle acceleration, High Energy Physics::Experiment, Field theory (psychology), Quantum field theory, Nuclear Experiment, Engineering (miscellaneous)
Abstract

Data on the production of the neutralK*(892) resonances at lowpt by 200 GeVK− and π− is compared with the predictions of various models based on the quark parton model of hadrons.

Published
1990

2. Inclusive $$\bar K^{0*} (892)$$ andK 0*(892) production on silicon by 200 GeVK − andπ −

Author

H. Palka, E. Belau, E. Higon, J. Richardson, D. P. Kelsey, S. Watts, P. Weilhammer, P. Gras, H.G.J.M. Tiecke, H. Dietl, G. de Rijk, M. Rozanska, M. Turala, M. Bosman, L. W. Wiggers, A. R. Gillman, M. Pepe, G. Waltermann, D. Bucholz, Z. Hajduk, W. Männer, V. Castillo, S. Kwan, H. Becker, B.D. Hyams, Fred Wickens, K. Rybicki, J. Kemmer, T. Böhringer, B. Lücking, T. Zeludziewicz, H.F. Fawcett, E. Neugebauer, J. Malos, G. Polok, U. Stierlin, S. Barlag, R. Klanner, R. J. Tapper, G. Lütjens, T. Gooch, S. Gill, G. Lutz, C. Daum, R.S. Gilmore, U. Kötz, H. J. Seebrunner, V. Chabaud, A. Wylie, and C.J.S. Damerell

Subjects
Physics, Nuclear physics, Physics and Astronomy (miscellaneous), Silicon, chemistry, Transverse momentum, chemistry.chemical_element, Atomic physics, Engineering (miscellaneous)
Abstract

The production of the neutralK− (892) resonances by 200 GeVK− andπ− has been studied over the kinematic range 0.0

Published
1990

3. Upper limits for charm production in 150 GeV p-Be interactions

Author

H.G.J.M. Tiecke, T. Zeludziewicz, M. Rozanska, L. W. Wiggers, Martine Bosman, M. Turala, Robert Klanner, B.D. Hyams, Fred Wickens, G. Waltermann, W. Männer, Hans Dijkstra, G. Polok, Rowan Bailey, G. Lütjens, W. Spierenburg, G. Lutz, C. Daum, V. Chabaud, C.J.S. Damerell, R. J. Tapper, U. Stierlin, R.S. Gilmore, A. R. Gillman, J. Malos, H. J. Seebrunner, C. Hardwick, A. Dwurazny, A. Wylie, L. Görlich, P. Weilhammer, K. Rybicki, T. Böhringer, W. Hoogland, Z. Hajduk, H. Becker, Jos Vermeulen, Marcos Cerrada, and E. Belau

Subjects
Systematic error, Physics, Nuclear and High Energy Physics, Particle physics, Meson, Nuclear Theory, Hadron, Large aperture, Electron, Charmed baryons, Nuclear physics, High Energy Physics::Experiment, Nuclear Experiment, Nucleon
Abstract

A search has been made for the hadronic production of charmed baryons and mesons with a large aperture forward magnetic spectrometer using 150 GeV protons originating from the CERN-SPS. A prompt electron trigger was used as a signature for charm. Upper limits at 90% confidence level have been obtained for the production of Λ c + D 0 , D 0 D + and D − : σ(Λ c ) ⩽ 8 μ b , σ( D 0 ) ⩽ 64 μ b , σ( D 0 ) b , σ( D + ) ⩽ 51 μ b and σ( D − ) ⩽ 49 μ b per nucleon, assuming linear A dependence. Systematic errors due to uncertainties in branching ratios and to model dependence of the acceptance calculation are discussed.

Published
1984

4. Measurement of mass and lifetime of hadronically produced charmed F mesons

Author

C.J.S. Damerell, Fred Wickens, A. R. Gillman, C. Daum, U. Koetz, Martine Bosman, C. De Rijk, H.G.J.M. Tiecke, Stephen Watts, R. J. Tapper, E. Belau, E. Neugebauer, G. Lutz, V. Chabaud, U. Stierlin, T. Zeludziewicz, C. Hardwick, R. Klanner, M. Rozanska, Z. Hajduk, L. W. Wiggers, A. Wylie, Hans Dijkstra, H. J. Seebrunner, M. Turala, Rowan Bailey, G. Waltermann, W. Männer, K. Rybicki, T. Böhringer, B.D. Hyams, P. Weilhammer, G. Lütjens, R.S. Gilmore, W. Hoogland, J. Malos, and S. Gill

Subjects
Physics, Nuclear and High Energy Physics, Particle physics, Meson, Spectrometer, Physics::Instrumentation and Detectors, Nuclear Theory, chemistry.chemical_element, law.invention, Nuclear physics, Telescope, chemistry, law, High Energy Physics::Experiment, Beryllium, Nuclear Experiment, Particle Physics - Experiment, Silicon microstrip detectors
Abstract

We have observed three unique decays of F mesons into KK π and five unique decays of D mesons into KK π using the NA11 spectrometer together with a telescope of high-resolution silicon microstrip detectors. The mass value obtained for the F meson is (1975 ± 4) MeV, the lifetime (3.2± 1.3 3..0 )×10 −13 s.

Published
1984

5. Joint production of ? mesons and? �,? 0,p, $$\bar p$$ ,K s 0 andK � in hadronic interactions

Author

R. J. Tapper, E. Belau, H. Palka, Z. Hajduk, C.J.S. Damerell, H. J. Seebrunner, H.G.J.M. Tiecke, U. Stierlin, A. R. Gillman, R. Klanner, A. Wylie, S. Kwan, M. Turala, S. Watts, C. Daum, C. Hardwick, Fred Wickens, M. Pepé, G. Lütjens, V. Chabaud, R. Bailey, L. W. Wiggers, Hans Dijkstra, Martine Bosman, P. Weilhammer, J. Richardson, R.S. Gilmore, B.D. Hyams, G. de Rijk, G. Waltermann, W. Männer, U. Kötz, K. Rybicki, T. Böhringer, E. Neugebauer, G. Lutz, T. Zeludziewicz, S. Gill, J. Malos, and W. Hoogland

Subjects
Physics, Physics and Astronomy (miscellaneous), Meson, High Energy Physics::Phenomenology, Nuclear Theory, Hadron, Parton, Nuclear physics, High Energy Physics::Experiment, Production (computer science), Nuclear Experiment, Engineering (miscellaneous), Joint (geology), Bar (unit)
Abstract

The joint production of ϕ mesons andπ ±,π 0,p, $$\bar p$$ ,K 0 andK ± is investigated using a sample of 600,000 inclusive ϕ meson events obtained in hadron Be interactions with incidentπ ±,p, $$\bar p$$ andK ± beams. Evidence is presented for the joint production of ϕ mesons and strange particles produced with non-strange incident beams. With incidentK ± beam the number of additional strange particles is suppressed. The results are found to be in agreement with the qualitative predictions of a parton fusion model. The comparison with the Lund model for lowp T processes is fair.

Published
1986

6. A-dependence study of inclusive ϑ production

Author

H. J. Seebrunner, Martine Bosman, G. Lütjens, A. Wylie, W. Hoogland, C. Daum, Stephen Watts, R. J. Tapper, V. Chabaud, B.D. Hyams, U. Stierlin, E. Neugebauer, Z. Hajduk, M. Turala, R. Klanner, R.S. Gilmore, G. Lutz, E. Belau, J. Malos, S. Gill, U. Koetz, C. Hardwick, T. Zeludziewicz, Hans Dijkstra, G. Waltermann, W. Männer, H.G.J.M. Tiecke, G. de Rijk, A. R. Gillman, P. Weilhammer, M. Rozanska, L. W. Wiggers, K. Rybicki, T. Böhringer, R. Bailey, Fred Wickens, and C.J.S. Damerell

Subjects
Physics, Physics and Astronomy (miscellaneous), chemistry, Meson, Analytical chemistry, Tantalum, chemistry.chemical_element, Elementary particle, Production (computer science), Beryllium, Atomic physics, Engineering (miscellaneous)
Abstract

TheA-dependence of the inclusive ϑ meson cross-section is measured using two target materials, beryllium and tantalum, in the kinematic range 0

Published
1984

7. Measurement of the lifetime of charged and neutralD mesons with high resolution silicon strip detectors

Author

M. Rozanska, A. R. Gillman, E. Belau, L. W. Wiggers, W. Hoogland, A. Wylie, Martine Bosman, C. Hardwick, G. de Rijk, J. Richardson, J. Kemmer, U. Stierlin, R. Klanner, Z. Hajduk, H. Palka, E. Neugebauer, R. Bailey, V. Chabaud, Hans Dijkstra, G. Lutz, S. Kwan, P. Weilhammer, J. Malos, U. Kötz, K. Rybicki, T. Böhringer, M. Turala, Fred Wickens, S. Gill, T. Zeludziewicz, C.J.S. Damerell, M. Pepé, H.G.J.M. Tiecke, G. Lütjens, B.D. Hyams, R. J. Tapper, G. Waltermann, W. Männer, R.S. Gilmore, H. J. Seebrunner, and C. Daum

Subjects
Physics, Particle physics, Physics and Astronomy (miscellaneous), Silicon, chemistry, Meson, chemistry.chemical_element, High resolution, High Energy Physics::Experiment, Elementary particle, Atomic physics, Nuclear Experiment, Engineering (miscellaneous)
Abstract

We have measured the lifetime of charged and neutralD mesons using high resolution silicon strip detectors in the NA11 spectrometer at the CERN SPS. TheD mesons were produced in 200 GeV π− interactions. We obtain a value of $$\tau _{D^ \pm } = \left( {10.6\begin{array}{*{20}c} { + 3.6} \\ { - 2.4} \\ \end{array} } \right) \cdot 10^{ - 13} s$$ based on 28, and $$\tau _{D^0 } = \left( {3.7\begin{array}{*{20}c} { + 1.0} \\ { - 0.7} \\ \end{array} } \right) \cdot 10^{ - 13} s$$ based on 26 fully reconstructed decays for the lifetime of the charged and the neutralD meson, respectively. For the ratio $$\tau _{D^ \pm } /\tau _{D^0 }$$ we find 2.8 −0.8 +1.1 .

Published
1985

8. Measurement ofD meson production in 200 GeV π−-Be interactions

Author

U. Kötz, H. J. Seebrunner, Fred Wickens, H.G.J.M. Tiecke, E. Belau, W. Hoogland, E. Neugebauer, G. Lutz, J. Richardson, A. Wylie, G. Waltermann, W. Männer, R. J. Tapper, Z. Hajduk, M. Rozanska, L. W. Wiggers, Martine Bosman, U. Stierlin, S. Kwan, R. Klanner, S. Gill, C.J.S. Damerell, R.S. Gilmore, P. Weilhammer, C. Daum, G. de Rijk, R. Bailey, V. Chabaud, J. Malos, M. Turala, K. Rybicki, T. Böhringer, M. Pepé, G. Lütjens, A. R. Gillman, T. Zeludziewicz, H. Paŀka, B.D. Hyams, J. Kemmer, Hans Dijkstra, and C. Hardwick

Subjects
Nuclear physics, Physics, Single electron, Particle physics, Meson production, Physics and Astronomy (miscellaneous), Meson, Elementary particle, Quantum field theory, Engineering (miscellaneous), Silicon microstrip detectors, Vertex (geometry)
Abstract

We have measured the differential and total cross sections ofD meson production in 200 GeV π−-beryllium interactions, using a sample of 48 fully reconstructed and nearly background-freeD mesons in the decay channelsK∓π±,K∓π±π± andK∓π∓π±π±. A single electron trigger has been used to select events containing a pair of charmed particles. A vertex telescope of 6 silison microstrip detectors allowed the reconstruction of tracks of charged secondaries and the reconstruction of primary and decay vertices with high precision. The ratio of branching fractions for\(\mathop {D^0 }\limits^{( - )} \to K^ \mp \pi ^ \pm \) to\(\mathop {D^0 }\limits^{( - )} \to K^ \mp \pi ^ \mp \pi ^ \pm \pi ^ \pm \), and an upper limit for\(D^0 - \bar D^0 \) mixing are presented.

Published
1986

9. InclusiveK *0(890) and $$\bar K*^0 $$ (890) production on beryllium byK − and π− at 175 GeV

Author

Z. Hajduk, C.J.S. Damerell, E. Belau, W. Hoogland, Jos Vermeulen, V. Chabaud, C. Dauin, G. Waltermann, A. Dwurazny, K. Rybicki, R. Bailey, Martine Bosman, T. Böhringer, G. Polok, R.S. Gilmore, G. Lutz, A. Wylie, A. R. Gillman, W. Männer, Marcos Cerrada, U. Stierlin, L. Görlich, R. J. Tappel, D. G. Bardsley, R. Klanner, J. Malos, T. Zeludziewicz, B.D. Hyams, G. Lütjens, M. Turala, W. Spierenburg, H.G.J.M. Tiecke, P. Weilhammer, Hans Dijkstra, G. de Rijk, C. Hardwick, M. Rozanska, L. W. Wiggers, H. J. Seebrunner, and Fred Wickens

Subjects
Physics, Quark, Particle physics, Physics and Astronomy (miscellaneous), Hydrogen, Hadron, chemistry.chemical_element, Nuclear physics, symbols.namesake, chemistry, symbols, Feynman diagram, Production (computer science), Beryllium, Engineering (miscellaneous), Beam (structure), Bar (unit)
Abstract

The reactions $$K^ - Be \to {}^(\bar K^) *^0 (890)X,\pi ^ - Be \to {}^(\bar K^) *^0 (890)X$$ , have been studied in a 175 GeV unseparated hadron beam in the kinematic range 0

Published
1984

10. Inclusive $\phi$ meson production, the parton fusion model and strange quark structure functions

Author

Martine Bosman, U. Stierlin, R. Klanner, U. Kötz, A. R. Gillman, H. Palka, B.D. Hyams, R. J. Tapper, Fred Wickens, A. Wylie, S. Gill, C.J.S. Damerell, S. Watts, R. Bailey, E. Belau, L. W. Wiggers, C. Hardwick, H.G.J.M. Tiecke, H. J. Seebrunner, M. Pepé, V. Chabaud, M. Turala, R.S. Gilmore, C. Daum, Hans Dijkstra, G. Lütjens, J. Malos, W. Hoogland, E. Neugebauer, G. Lutz, T. Zeludziewicz, G. Waltermann, W. Männer, Z. Hajduk, S. Kwan, G. de Rijk, J. Richardson, K. Rybicki, T. Böhringer, and P. Weilhammer

Subjects
Quark, Physics, Particle physics, Fusion, Strange quark, Valence (chemistry), Physics and Astronomy (miscellaneous), Meson, High Energy Physics::Phenomenology, Nuclear Theory, Structure function, Hadron, Parton, Nuclear physics, High Energy Physics::Experiment, Nuclear Experiment, Engineering (miscellaneous), Particle Physics - Experiment
Abstract

The parton fusion model is used to describe the longitudinal differential cross section (dσ/dxF) of hadronic ϕ production. Thedσ/dxF for ϕ production in π±p,\(\mathop p\limits^{( - )} p\) andK±p interactions is evaluated in the model by using structure functions for the constituents of the interacting particles. A comparison between the model and high statistics data in the Feynmanx range 0.0

Published
1986

11. Hadronic $\phi$ production and the Lund model for low p$_{T}$ interactions

Author

R. J. Tapper, M. Turala, B.D. Hyams, Hans Dijkstra, G. de Rijk, C.J.S. Damerell, H.G.J.M. Tiecke, P. Weilhammer, J. Richardson, S. Gill, U. Kötz, Fred Wickens, E. Belau, C. Hardwick, S. Watts, R. Bailey, G. Waltermann, K. Rybicki, A. Wylie, W. Männer, T. Böhringer, Martine Bosman, T. Zeludziewicz, L. W. Wiggers, Z. Hajduk, C. Daum, S. Kwan, J. Malos, E. Neugebauer, M. Pepé, G. Lutz, V. Chabaud, U. Stierlin, R. Klanner, A. R. Gillman, G. Lütjens, R.S. Gilmore, H. Palka, W. Hoogland, and H. J. Seebrunner

Subjects
Physics, Particle physics, Physics and Astronomy (miscellaneous), Meson, Hadron, Elementary particle, Nuclear physics, Particle acceleration, Momentum, Field theory (psychology), Quantum field theory, Engineering (miscellaneous), Particle Physics - Experiment, Bar (unit)
Abstract

Experimental results on inclusive ϕ production are compared with the Lund model for lowp T hadronic interactions. The data is based on a sample of 600,000 ϕ mesons in the kinematic rangep T

Published
1986

12. Semileptonic decays of charmed $D^{\pm}$ meson: measurement of the lifetime and the cross-section

Author

C. Doum, L. W. Wiggers, H. J. Seebrunner, Fred Wickens, G. Waltermann, U. Stierlin, W. Männer, T. Gooch, S. Gill, Martine Bosman, S. Watts, G. de Rijk, R. Klanner, U. Kötz, T. Zeludziewicz, H.G.J.M. Tiecke, J. Kemmer, A. R. Gillman, B.D. Hyams, C.J.S. Damerell, J. Malos, E. Belau, K. Rybicki, M. Pepé, T. Böhringer, P. Weilhammer, A. Wylie, M. Turala, V. Chabaud, J. Richardson, Z. Hajduk, H. Palka, R. Bailey, S. Kwan, H. Becker, R.S. Gilmore, G. Lütjens, E. Neugebauer, G. Lutz, Hans Dijkstra, and C. Hardwick

Subjects
Physics, Particle physics, Physics and Astronomy (miscellaneous), Meson, Silicon, Spectrometer, chemistry.chemical_element, High resolution, Elementary particle, Vertex (geometry), law.invention, Telescope, chemistry, law, Quantum field theory, Engineering (miscellaneous), Particle Physics - Experiment
Abstract

Using the ACCMOR spectrometer equipped with a vertex telescope of high resolution silicon microstrip detectors 74 decays\(D^ + \to \bar K^{*0} (892)e^ + v_e \) (andcc.) withx F

Published
1987

13. High statistics inclusive $\phi$ meson production at SPS energies

Author

Z. Hajduk, A. Wylie, R.S. Gilmore, E. Belau, S. Kwan, C.J.S. Damerell, C. Daum, G. Waltermann, S. Watts, W. Männer, G. de Rijk, V. Chabaud, H. Palka, Martine Bosman, A. R. Gillman, T. Zeludziewicz, G. Lütjens, R. Bailey, U. Stierlin, J. Richardson, R. Klanner, J. Malos, C. Hardwick, H.G.J.M. Tiecke, B.D. Hyams, K. Rybicki, S. Gill, P. Weilhammer, T. Böhringer, R. J. Tapper, E. Neugebauer, W. Hoogland, G. Lutz, Hans Dijkstra, M. Turala, U. Kötz, Fred Wickens, L. W. Wiggers, M. Pepé, H. J. Seebrunner, and B Kisielewski

Subjects
Quark, Physics, Density matrix, Particle physics, Valence (chemistry), Physics and Astronomy (miscellaneous), Meson, Mass distribution, Nuclear Theory, High Energy Physics::Phenomenology, Elementary particle, Strangeness, Nuclear physics, High Energy Physics::Experiment, Quantum field theory, Nuclear Experiment, Engineering (miscellaneous), Particle Physics - Experiment
Abstract

Inclusive ϕ meson production has been measured for 100 GeV/c and 200 GeV/c incident π−,\(\bar p\) andK−, and for 120 GeV/c and 200 GeV/c incident π+,p andK+, using a Be target. A total of 630,000 ϕ mesons has been recorded in the kinematic range 0

Published
1986

15. Axonal Degeneration of the Vagus Nerve in Parkinson's Disease-A High-Resolution Ultrasound Study.

Author

Pelz JO, Belau E, Fricke C, Classen J, and Weise D

Abstract

Background: Recent histopathological studies revealed degeneration of the dorsal motor nucleus early in the course of Parkinson's disease (PD). Degeneration of the vagus nerve (VN) axons following neurodegeneration of brainstem vagal nuclei should be detectable by high-resolution ultrasound (HRUS) as a thinning of the VNs. Methods: We measured both VNs cross-sectional area (VN-CSA) of 35 patients with PD and 35 age- and sex-matched healthy controls at the level of the thyroid gland using HRUS. Results: On both sides, the VN-CSA was significantly smaller in PD patients than in controls (right: 2.1 ± 0.4 vs. 2.3 ± 0.5 mm2, left 1.5 ± 0.4 vs. 1.8 ± 0.4 mm2; both p < 0.05). There was no correlation between the right or left VN-CSA and age, the Hoehn & Yahr stage, disease duration, the motor part of the Unified Parkinson's Disease Rating Scale score, the Montreal Cognitive Assessment score, or the Non-motor Symptoms Questionnaire, and Scale for Parkinson's disease score including its gastrointestinal domain. Conclusions: These findings provide evidencethat atrophy of the VNs in PD patients can be detected in-vivo by HRUS.

Published
2018
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16. Full validation of therapeutic antibody sequences by middle-up mass measurements and middle-down protein sequencing.

Author

Resemann A, Jabs W, Wiechmann A, Wagner E, Colas O, Evers W, Belau E, Vorwerg L, Evans C, Beck A, and Suckau D

Subjects
Antibodies, Monoclonal chemistry, Cetuximab chemistry, Humans, Natalizumab chemistry, Panitumumab, Antibodies, Monoclonal genetics, Cetuximab genetics, Natalizumab genetics, Sequence Analysis, Protein methods
Abstract

The regulatory bodies request full sequence data assessment both for innovator and biosimilar monoclonal antibodies (mAbs). Full sequence coverage is typically used to verify the integrity of the analytical data obtained following the combination of multiple LC-MS/MS datasets from orthogonal protease digests (so called "bottom-up" approaches). Top-down or middle-down mass spectrometric approaches have the potential to minimize artifacts, reduce overall analysis time and provide orthogonality to this traditional approach. In this work we report a new combined approach involving middle-up LC-QTOF and middle-down LC-MALDI in-source decay (ISD) mass spectrometry. This was applied to cetuximab, panitumumab and natalizumab, selected as representative US Food and Drug Administration- and European Medicines Agency-approved mAbs. The goal was to unambiguously confirm their reference sequences and examine the general applicability of this approach. Furthermore, a new measure for assessing the integrity and validity of results from middle-down approaches is introduced - the "Sequence Validation Percentage." Full sequence data assessment of the 3 antibodies was achieved enabling all 3 sequences to be fully validated by a combination of middle-up molecular weight determination and middle-down protein sequencing. Three errors in the reference amino acid sequence of natalizumab, causing a cumulative mass shift of only -2 Da in the natalizumab Fd domain, were corrected as a result of this work.

Published
2016
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17. Imaging mass spectrometry analysis of renal amyloidosis biopsies reveals protein co-localization with amyloid deposits.

Author

Casadonte R, Kriegsmann M, Deininger SO, Amann K, Paape R, Belau E, Suckau D, Fuchser J, Beckmann J, Becker M, and Kriegsmann J

Subjects
Amyloidosis diagnosis, Apolipoproteins E analysis, Humans, Immunoglobulin Light Chains analysis, Plaque, Amyloid diagnosis, Serum Amyloid A Protein analysis, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Tandem Mass Spectrometry, Vitronectin analysis, Amyloid analysis, Amyloidosis pathology, Kidney pathology, Plaque, Amyloid pathology
Abstract

Amyloidosis is a heterogeneous group of protein misfolding diseases characterized by deposition of amyloid proteins. The kidney is frequently affected, especially by immunoglobulin light chain (AL) and serum amyloid A (SAA) amyloidosis as the most common subgroups. Current diagnosis relies on histopathological examination, Congo red staining, or electron microscopy. Subtyping is done by immunohistochemistry; however, commercially available antibodies lack specificity. The purpose of this study was to identify and map amyloid proteins in formalin-fixed paraffin-embedded tissue sections using matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis in an integrated workflow. Renal amyloidosis and non-amyloidosis biopsies were processed for histological and MS analysis. Mass spectra corresponding to the congophilic areas were directly linked to the histological and MS images for correlation studies. Peptides for SAA and AL were detected by MALDI IMS associated to Congo red-positive areas. Sequence determination of amyloid peptides by LC-MS/MS analysis provided protein distribution and identification. Serum amyloid P component, apolipoprotein E, and vitronectin proteins were identified in both AA and AL amyloidosis, showing a strong correlation with Congo red-positive regions. Our findings highlight the utility of MALDI IMS as a new method to type amyloidosis in histopathological routine material and characterize amyloid-associated proteins that may provide insights into the pathogenetic process of amyloid formation.

Published
2015
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