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327 results on '"Diverio, D"'

27. Chronic phase chronic myeloid leukemia patients who failed interferon alpha and switched to imatinib: Long-term 9-year follow-up of 134 patients

Author

Breccia, M, Latagliata, R, Molica, M, Colafigli, G, Mancini, M, Diverio, D, Tafuri, Agostino, and Alimena, Giuliana

Subjects
Adult, Male, Adolescent, Drug Substitution, Dasatinib, Interferon-alpha, Antineoplastic Agents, Middle Aged, Survival Analysis, Piperazines, Thiazoles, Pyrimidines, Drug Resistance, Neoplasm, Benzamides, Leukemia, Myeloid, Chronic-Phase, Imatinib Mesylate, Humans, Female, Aged, Follow-Up Studies
Published
2015

34. AIDA 0493 protocol for newly diagnosed acute promyelocytic leukemia: very long-term results and role of maintenance

Author

Avvisati, G, Lo Coco, F, Paoloni, Fp, Petti, Mc, Diverio, D, Vignetti, M, Latagliata, R, Specchia, G, Baccarani, M, Di Bona, E, Fioritoni, G, Marmont, F, Rambaldi, A, DI RAIMONDO, Francesco, Kropp, Mg, Pizzolo, G, Pogliani, Em, Rossi, G, Cantore, N, Nobile, F, Gabbas, A, Ferrara, F, Fazi, P, Amadori, S, Mandelli, F, Gimema, Aieop, and EORTC Cooperative Groups

Published
2011

35. Efficacia dell’imatinib in bambini ed adolescenti con leucemia mieloide cronica philadelphia positiva trattati in fase cronica. [Imatinib effective in children and adolescents with Ph+ chronic myeloid leukemia treated in chronic phase]

Author

Giona, Fiorina, Putti, M. C., Santoro, N., Gottardi, E., Ladogana, S., Micalizzi, C., Iaria, C., Consarrino, C., Burnelli, R., Rea, M., Moleti, M. L., Testi, Anna Maria, Cavillo, M., Cecinati, V., Nanni, M., Messina, C., Diverio, D., Varotto, S., Biondi, A., Pession, A., Saglio, G., and Foà, R.

Published
2010

36. Front-line treatment of acute promyelocytic leukemia with AIDA induction followed by risk-adapted consolidation for adults younger than 61 years: results of the AIDA-2000 trial of the GIMEMA Group

Author

LO COCO, F, Avvisati, G, Vignetti, M, Breccia, M, Gallo, E, Rambaldi, A, Paoloni, F, Fioritoni, G, Ferrara, F, Specchia, G, Cimino, G, Diverio, D, Borlenghi, E, Martinelli, G, DI RAIMONDO, Francesco, DI BONA, E, Fazi, P, Peta, A, Bosi, A, Carella, Am, Fabbiano, F, Pogliani, Em, Petti, Mc, Amadori, S, Mandelli, F, and ITALIAN GIMEMA COOPERATIVE GROUP

Published
2010

47. Denaturing high-performance liquid chromatography: a valid approach for identifying NPM1 mutations in Acute Myeloid Leukemia

Author

Roti, Giovanni, Rosati, R, Bonasso, R, Gorello, P, Diverio, D, Martelli, Mf, Falini, B, Mecucci, Cristina, and Gruppo Italiano Malattie Ematologiche dell' Adulto Working Party

Subjects
Cytoplasm, Leukemia, Myeloid, Acute, Base Sequence, hemic and lymphatic diseases, Molecular Sequence Data, Mutation, Humans, Nuclear Proteins, Amino Acid Sequence, Nucleophosmin, Chromatography, High Pressure Liquid, Regular Articles
Abstract

NPM1 gene mutations are the most frequent genetic lesion in the 60% of adult acute myeloid leukemias (AMLs) with normal karyotype and no evidence of typical fusion genes (BCR/ABL1, PML/RARA, AML1/ETO, CBFB/MYH11, DEK/CAN). Using direct sequencing we previously identified six different heterozygous mutants within exon 12 encoding the nucleophosmin C-terminus. Because of these mutations the shuttling protein nucleophosmin is aberrantly delocalized in the cytoplasm of leukemic cells (NPMc+). Here, we designed and tested a denaturing high-performance liquid chromatography (DHPLC) assay to detect NPM1 mutated variants. To assess specificity, sensitivity, reliability, and reproducibility, we analyzed DNA from 120 primary adult AMLs and compared DHPLC results with immunohistochemistry and sequencing. All electropherogram profiles in the 26 NPMc+ leukemias were different from the wild type, indicating 100% sensitivity. Sequencing categorized mutations A, B, and D, and all mutation A cases gave identical elution profiles. The other mutations showed typical chromatograms, with mutations B and D differing for one nucleotide. Elution profiles and sequencing also identified four new variants. Our results suggest that DHPLC detects NPM1mutations as well as direct sequencing and immunohistochemistry, providing a helpful approach in the diagnosis of NPMc+ AML.

Published
2006

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