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17. Adenosine triphosphate detection by controlled-release of carboxy fluorescein from mesoporous silica nanoparticles blocked with aptamer-based gold nanoparticles

Author

Ji, Xiaoting, Lv, Haoyuan, Zhang, Wei, and Ding, Caifeng

Subjects
Chemical properties, Nanoparticles -- Chemical properties, Fluorescein -- Chemical properties, ATP (Adenosine triphosphate) -- Chemical properties
Abstract

In recent years, mesoporous silica nanoparticles have attracted attention as a promising component of nanomachines [1, 2]. Due to Si-MPs' excellent biocompatibility [3] and multiple functionalization [4], they have been [...], In this paper, an adenosine-5'-triphosphate (ATP) controlled-release strategy to construct a fluorescence sensing platform has been designed. In the sensing platform, because of ATP aptamer and single-stranded DNA (ssDNA)-linked mesoporous silica nanoparticles (Si-MPs) were hybridized, the pores of SiMPs were blocked with Au nanoparticles (AuNPs) modified with ATP aptamer. Carboxy fluorescein was plugged in channels of Si-MPs. In the presence of target molecule ATP, the ATP aptamer combined with ATP and the AuNPs got away from the pore of the surface of Si-MPs modified by ssDNA. 5-Carboxyfluorescein molecule was released to allow the fluorescent detection. By monitoring the fluorescence at 518 nm, ATP could be quantitatively detected with a detection limit of 6 x [10.sup.-8] M. The linear response range was 6 x [10.sup.-8] to 1 x [10.sup.-6] M. This assay was also able to discriminate ATP from its analogs. The controlled-release aptamer-based biosensor could have an effective application in human breast cancer MCF-7 cells. Keywords: adenosine triphosphate, aptamer, mesoporous silica nanoparticles, controlled-release, fluorescence

Published
2017
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26. Meet our Editor (Analytical Chemistry)

Author

Ding Caifeng, Liang Ying, Tang Jianjun, Liu Niannian, Cho William, Yang Le, Mei Meng, and Wang Yinglong

Subjects
Management science, Computer science, General Earth and Planetary Sciences, Analytical Chemistry (journal), General Environmental Science
Published
2021
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29. Preparation and properties of bisphenol A sensor based on multiwalled carbon nanotubes/Li4Ti5O12-modified electrode

Author

Ding Caifeng, Yuan-xiang Gu, Wei Wang, Jun Wan, and Xiao Yang

Subjects
Detection limit, Nanotube, Materials science, Scanning electron microscope, General Chemical Engineering, Composite number, General Engineering, General Physics and Astronomy, Electrochemical gas sensor, Adsorption, Chemical engineering, Specific surface area, Electrode, General Materials Science, Composite material
Abstract

A new electrochemical sensor based on the multiwalled carbon nanotube (MWCNT)/Li4Ti5O12-modified glassy carbon electrode for determination of bisphenol A (BPA) was developed by modifying the surface of a glassy carbon electrode with the prepared composite Li4Ti5O12/MWCNTs. The morphology and structure of the composite were characterized by infrared spectroscopy and scanning electron microscope. The obtained electrochemical sensor Li4Ti5O12/MWCNTs/GCE takes advantages of large specific surface area of the Li4Ti5O12 nanostructure, excellent adsorption properties, and electrochemical properties of MWCNTs. Under optimal conditions, the response of the proposed sensor to BPA was linear in the BPA concentration range from 1.0 × 10−7 to 1.0 × 10−5 mol L−1 and the detection limit was of 7.8 × 10−8 mol L−1 according to the 3σ rule. The results revealed that the newly developed sensor exhibited high sensitivity and selectivity, excellent electrochemical performance, and fast response to BPA, allowing it to be used for the detection of BPA in actual samples.

Published
2014
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