1. EFFECT OF FOLLITROPIN ON ADENYLATE CYCLASE ACTIVITY AND ON FSH RECEPTOR MRNA EXPRESSION DURING THE OVARIAN CYCLE OF PODARCIS SICULA
- Author
-
Elio Parisi, Roberta De Stasio, Lucia Borrelli, Silvana Filosa, Filosa, Silvana, Borrelli, L, DE STASIO, R, and Parisi, E.
- Subjects
endocrine system ,medicine.medical_specialty ,Adenylate kinase ,Follitropin ,Ovary ,Biology ,Cyclase ,medicine.anatomical_structure ,Endocrinology ,Internal medicine ,medicine ,heterocyclic compounds ,Animal Science and Zoology ,Northern blot ,Vitellogenesis ,Follicle-stimulating hormone receptor ,Cyclase activity ,hormones, hormone substitutes, and hormone antagonists - Abstract
The relationship is reported between adenylate cyclase sensitivity to follitropin and FSH receptor mRNA expression in the ovary of the lizard Podarcis sicula. The results demonstrate that ovarian adenylate cyclase responsiveness to FSH parallels ovarian functions, being maximal during the ovulatory period. Ovarian sensitivity to FSH is also related to oocyte growth and vitellogenesis. Northern blot analyses revealed that the FSH receptor mRNAs were maximally expressed in vitellogenic oocytes during the reproductive period. In lizard treated in vivo with FSH during the pre‐ovulatory period, adenylate cyclase became refractory to further FSH stimulation 2 h after treatment, but sensitivity to the hormone was restored after two weeks. Nevertheless, the expression level of FSH receptor mRNAs was significantly enhanced in these animals. The conclusion is that in lizard the ovarian cycle is controlled by follitropin, FSH receptor and adenylate cyclase.
- Published
- 2004