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1. L’espressione e la percezione delle emozioni vocali in apprendenti di Italiano L2

Author

De Marco, Anna and Paone, Emanuela

Subjects
Language. Linguistic theory. Comparative grammar, P101-410
Abstract

This study explored the expression and the perception of vocal emotions in L2 learners of Italian coming from different countries. We carried out an acoustic analysis of learners’ emotional speech in their L1s and in Italian, to verify to what extent their L1 influenced their productions in L2. To test learners’ ability to decode vocal emotions expressed by natives, we administered a perception test containing emotional stimuli in Italian; to verify the effectiveness of learners’ emotional speech, we administered a perception test to Italian listeners. Results revealed that learners’ productions were influenced by their L1s, consequently Italians experienced difficulties in decoding them. Learners encountered the same difficulties in identifying natives’ emotional speech. Such awareness could be useful for L2 teaching practices.

Published
2014
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2. A pan-influenza antibody inhibiting neuraminidase via receptor mimicry

Author

Momont, Corey, Dang, Ha V., Zatta, Fabrizia, Hauser, Kevin, Wang, Caihong, di Iulio, Julia, Minola, Andrea, Czudnochowski, Nadine, De Marco, Anna, Branch, Kaitlin, Donermeyer, David, Vyas, Siddhant, Chen, Alex, Ferri, Elena, Guarino, Barbara, Powell, Abigail E., Spreafico, Roberto, Yim, Samantha S., Balce, Dale R., Bartha, Istvan, Meury, Marcel, Croll, Tristan I., Belnap, David M., Schmid, Michael A., Schaiff, William Timothy, Miller, Jessica L., Cameroni, Elisabetta, Telenti, Amalio, Virgin, Herbert W., Rosen, Laura E., Purcell, Lisa A., Lanzavecchia, Antonio, Snell, Gyorgy, Corti, Davide, and Pizzuto, Matteo Samuele

Published
2023
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3. SARS-CoV-2 RBD antibodies that maximize breadth and resistance to escape

Author

Starr, Tyler N, Czudnochowski, Nadine, Liu, Zhuoming, Zatta, Fabrizia, Park, Young-Jun, Addetia, Amin, Pinto, Dora, Beltramello, Martina, Hernandez, Patrick, Greaney, Allison J, Marzi, Roberta, Glass, William G, Zhang, Ivy, Dingens, Adam S, Bowen, John E, Tortorici, M Alejandra, Walls, Alexandra C, Wojcechowskyj, Jason A, De Marco, Anna, Rosen, Laura E, Zhou, Jiayi, Montiel-Ruiz, Martin, Kaiser, Hannah, Dillen, Josh R, Tucker, Heather, Bassi, Jessica, Silacci-Fregni, Chiara, Housley, Michael P, di Iulio, Julia, Lombardo, Gloria, Agostini, Maria, Sprugasci, Nicole, Culap, Katja, Jaconi, Stefano, Meury, Marcel, Dellota Jr, Exequiel, Abdelnabi, Rana, Foo, Shi-Yan Caroline, Cameroni, Elisabetta, Stumpf, Spencer, Croll, Tristan I, Nix, Jay C, Havenar-Daughton, Colin, Piccoli, Luca, Benigni, Fabio, Neyts, Johan, Telenti, Amalio, Lempp, Florian A, Pizzuto, Matteo S, Chodera, John D, Hebner, Christy M, Virgin, Herbert W, Whelan, Sean PJ, Veesler, David, Corti, Davide, Bloom, Jesse D, and Snell, Gyorgy

Subjects
Medical Microbiology, Biomedical and Clinical Sciences, Immunology, Vaccine Related, Lung, Prevention, Biodefense, Pneumonia & Influenza, Emerging Infectious Diseases, Immunization, Pneumonia, Biotechnology, Development of treatments and therapeutic interventions, 5.1 Pharmaceuticals, Good Health and Well Being, Adult, Aged, Animals, Antibodies, Monoclonal, Antibodies, Viral, Antibody Affinity, Broadly Neutralizing Antibodies, COVID-19, COVID-19 Vaccines, Cell Line, Cricetinae, Cross Reactions, Epitopes, B-Lymphocyte, Female, Humans, Immune Evasion, Male, Mesocricetus, Middle Aged, Models, Molecular, SARS-CoV-2, Spike Glycoprotein, Coronavirus, Vaccinology, COVID-19 Drug Treatment, General Science & Technology
Abstract

An ideal therapeutic anti-SARS-CoV-2 antibody would resist viral escape1-3, have activity against diverse sarbecoviruses4-7, and be highly protective through viral neutralization8-11 and effector functions12,13. Understanding how these properties relate to each other and vary across epitopes would aid the development of therapeutic antibodies and guide vaccine design. Here we comprehensively characterize escape, breadth and potency across a panel of SARS-CoV-2 antibodies targeting the receptor-binding domain (RBD). Despite a trade-off between in vitro neutralization potency and breadth of sarbecovirus binding, we identify neutralizing antibodies with exceptional sarbecovirus breadth and a corresponding resistance to SARS-CoV-2 escape. One of these antibodies, S2H97, binds with high affinity across all sarbecovirus clades to a cryptic epitope and prophylactically protects hamsters from viral challenge. Antibodies that target the angiotensin-converting enzyme 2 (ACE2) receptor-binding motif (RBM) typically have poor breadth and are readily escaped by mutations despite high neutralization potency. Nevertheless, we also characterize a potent RBM antibody (S2E128) with breadth across sarbecoviruses related to SARS-CoV-2 and a high barrier to viral escape. These data highlight principles underlying variation in escape, breadth and potency among antibodies that target the RBD, and identify epitopes and features to prioritize for therapeutic development against the current and potential future pandemics.

Published
2021

4. Circulating SARS-CoV-2 spike N439K variants maintain fitness while evading antibody-mediated immunity

Author

Thomson, Emma C, Rosen, Laura E, Shepherd, James G, Spreafico, Roberto, da Silva Filipe, Ana, Wojcechowskyj, Jason A, Davis, Chris, Piccoli, Luca, Pascall, David J, Dillen, Josh, Lytras, Spyros, Czudnochowski, Nadine, Shah, Rajiv, Meury, Marcel, Jesudason, Natasha, De Marco, Anna, Li, Kathy, Bassi, Jessica, O’Toole, Aine, Pinto, Dora, Colquhoun, Rachel M, Culap, Katja, Jackson, Ben, Zatta, Fabrizia, Rambaut, Andrew, Jaconi, Stefano, Sreenu, Vattipally B, Nix, Jay, Zhang, Ivy, Jarrett, Ruth F, Glass, William G, Beltramello, Martina, Nomikou, Kyriaki, Pizzuto, Matteo, Tong, Lily, Cameroni, Elisabetta, Croll, Tristan I, Johnson, Natasha, Di Iulio, Julia, Wickenhagen, Arthur, Ceschi, Alessandro, Harbison, Aoife M, Mair, Daniel, Ferrari, Paolo, Smollett, Katherine, Sallusto, Federica, Carmichael, Stephen, Garzoni, Christian, Nichols, Jenna, Galli, Massimo, Hughes, Joseph, Riva, Agostino, Ho, Antonia, Schiuma, Marco, Semple, Malcolm G, Openshaw, Peter JM, Fadda, Elisa, Baillie, J Kenneth, Chodera, John D, Investigators, The ISARIC4C, Consortium, the COVID-19 Genomics UK, Rihn, Suzannah J, Lycett, Samantha J, Virgin, Herbert W, Telenti, Amalio, Corti, Davide, Robertson, David L, and Snell, Gyorgy

Subjects
Medical Microbiology, Biomedical and Clinical Sciences, Biological Sciences, Immunology, Pneumonia, Prevention, Infectious Diseases, Vaccine Related, Immunization, Pneumonia & Influenza, Biodefense, Biotechnology, Emerging Infectious Diseases, Lung, 2.1 Biological and endogenous factors, Aetiology, Infection, Good Health and Well Being, Angiotensin-Converting Enzyme 2, Antibodies, Neutralizing, Antibodies, Viral, COVID-19, Genetic Fitness, Humans, Immune Evasion, Mutation, Phylogeny, SARS-CoV-2, Spike Glycoprotein, Coronavirus, Virulence, ISARIC4C Investigators, COVID-19 Genomics UK (COG-UK) Consortium, N439K, Spike, monoclonal antibody escape, mutation, protein structure, receptor binding motif, variant, Medical and Health Sciences, Developmental Biology, Biological sciences, Biomedical and clinical sciences
Abstract

SARS-CoV-2 can mutate and evade immunity, with consequences for efficacy of emerging vaccines and antibody therapeutics. Here, we demonstrate that the immunodominant SARS-CoV-2 spike (S) receptor binding motif (RBM) is a highly variable region of S and provide epidemiological, clinical, and molecular characterization of a prevalent, sentinel RBM mutation, N439K. We demonstrate N439K S protein has enhanced binding affinity to the hACE2 receptor, and N439K viruses have similar in vitro replication fitness and cause infections with similar clinical outcomes as compared to wild type. We show the N439K mutation confers resistance against several neutralizing monoclonal antibodies, including one authorized for emergency use by the US Food and Drug Administration (FDA), and reduces the activity of some polyclonal sera from persons recovered from infection. Immune evasion mutations that maintain virulence and fitness such as N439K can emerge within SARS-CoV-2 S, highlighting the need for ongoing molecular surveillance to guide development and usage of vaccines and therapeutics.

Published
2021

5. Mapping Neutralizing and Immunodominant Sites on the SARS-CoV-2 Spike Receptor-Binding Domain by Structure-Guided High-Resolution Serology

Author

Piccoli, Luca, Park, Young-Jun, Tortorici, M Alejandra, Czudnochowski, Nadine, Walls, Alexandra C, Beltramello, Martina, Silacci-Fregni, Chiara, Pinto, Dora, Rosen, Laura E, Bowen, John E, Acton, Oliver J, Jaconi, Stefano, Guarino, Barbara, Minola, Andrea, Zatta, Fabrizia, Sprugasci, Nicole, Bassi, Jessica, Peter, Alessia, De Marco, Anna, Nix, Jay C, Mele, Federico, Jovic, Sandra, Rodriguez, Blanca Fernandez, Gupta, Sneha V, Jin, Feng, Piumatti, Giovanni, Lo Presti, Giorgia, Pellanda, Alessandra Franzetti, Biggiogero, Maira, Tarkowski, Maciej, Pizzuto, Matteo S, Cameroni, Elisabetta, Havenar-Daughton, Colin, Smithey, Megan, Hong, David, Lepori, Valentino, Albanese, Emiliano, Ceschi, Alessandro, Bernasconi, Enos, Elzi, Luigia, Ferrari, Paolo, Garzoni, Christian, Riva, Agostino, Snell, Gyorgy, Sallusto, Federica, Fink, Katja, Virgin, Herbert W, Lanzavecchia, Antonio, Corti, Davide, and Veesler, David

Subjects
Medical Microbiology, Biomedical and Clinical Sciences, Immunology, Clinical Research, Lung, Pneumonia & Influenza, Biodefense, Immunization, Prevention, Infectious Diseases, Pneumonia, Vaccine Related, Emerging Infectious Diseases, Infection, Good Health and Well Being, Angiotensin-Converting Enzyme 2, Antibodies, Monoclonal, Antibodies, Neutralizing, Antibodies, Viral, Antigen-Antibody Reactions, Betacoronavirus, Binding Sites, COVID-19, Coronavirus Infections, Epitope Mapping, Epitopes, Humans, Immunoglobulin A, Immunoglobulin G, Immunoglobulin M, Kinetics, Molecular Dynamics Simulation, Pandemics, Peptidyl-Dipeptidase A, Pneumonia, Viral, Protein Binding, Protein Domains, Protein Structure, Quaternary, SARS-CoV-2, Spike Glycoprotein, Coronavirus, coronaviruses, effector functions, immunity, neutralizing antibodies, Biological Sciences, Medical and Health Sciences, Developmental Biology, Biological sciences, Biomedical and clinical sciences
Abstract

Analysis of the specificity and kinetics of neutralizing antibodies (nAbs) elicited by SARS-CoV-2 infection is crucial for understanding immune protection and identifying targets for vaccine design. In a cohort of 647 SARS-CoV-2-infected subjects, we found that both the magnitude of Ab responses to SARS-CoV-2 spike (S) and nucleoprotein and nAb titers correlate with clinical scores. The receptor-binding domain (RBD) is immunodominant and the target of 90% of the neutralizing activity present in SARS-CoV-2 immune sera. Whereas overall RBD-specific serum IgG titers waned with a half-life of 49 days, nAb titers and avidity increased over time for some individuals, consistent with affinity maturation. We structurally defined an RBD antigenic map and serologically quantified serum Abs specific for distinct RBD epitopes leading to the identification of two major receptor-binding motif antigenic sites. Our results explain the immunodominance of the receptor-binding motif and will guide the design of COVID-19 vaccines and therapeutics.

Published
2020

6. Altered TMPRSS2 usage by SARS-CoV-2 Omicron impacts infectivity and fusogenicity

Author

Meng, Bo, Abdullahi, Adam, Ferreira, Isabella A. T. M., Goonawardane, Niluka, Saito, Akatsuki, Kimura, Izumi, Yamasoba, Daichi, Gerber, Pehuén Pereyra, Fatihi, Saman, Rathore, Surabhi, Zepeda, Samantha K., Papa, Guido, Kemp, Steven A., Ikeda, Terumasa, Toyoda, Mako, Tan, Toong Seng, Kuramochi, Jin, Mitsunaga, Shigeki, Ueno, Takamasa, Shirakawa, Kotaro, Takaori-Kondo, Akifumi, Brevini, Teresa, Mallery, Donna L., Charles, Oscar J., Bowen, John E., Joshi, Anshu, Walls, Alexandra C., Jackson, Laurelle, Martin, Darren, Smith, Kenneth G. C., Bradley, John, Briggs, John A. G., Choi, Jinwook, Madissoon, Elo, Meyer, Kerstin B., Mlcochova, Petra, Ceron-Gutierrez, Lourdes, Doffinger, Rainer, Teichmann, Sarah A., Fisher, Andrew J., Pizzuto, Matteo S., de Marco, Anna, Corti, Davide, Hosmillo, Myra, Lee, Joo Hyeon, James, Leo C., Thukral, Lipi, Veesler, David, Sigal, Alex, Sampaziotis, Fotios, Goodfellow, Ian G., Matheson, Nicholas J., Sato, Kei, and Gupta, Ravindra K.

Published
2022
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7. Broadly neutralizing antibodies overcome SARS-CoV-2 Omicron antigenic shift

Author

Cameroni, Elisabetta, Bowen, John E., Rosen, Laura E., Saliba, Christian, Zepeda, Samantha K., Culap, Katja, Pinto, Dora, VanBlargan, Laura A., De Marco, Anna, di Iulio, Julia, Zatta, Fabrizia, Kaiser, Hannah, Noack, Julia, Farhat, Nisar, Czudnochowski, Nadine, Havenar-Daughton, Colin, Sprouse, Kaitlin R., Dillen, Josh R., Powell, Abigail E., Chen, Alex, Maher, Cyrus, Yin, Li, Sun, David, Soriaga, Leah, Bassi, Jessica, Silacci-Fregni, Chiara, Gustafsson, Claes, Franko, Nicholas M., Logue, Jenni, Iqbal, Najeeha Talat, Mazzitelli, Ignacio, Geffner, Jorge, Grifantini, Renata, Chu, Helen, Gori, Andrea, Riva, Agostino, Giannini, Olivier, Ceschi, Alessandro, Ferrari, Paolo, Cippà, Pietro E., Franzetti-Pellanda, Alessandra, Garzoni, Christian, Halfmann, Peter J., Kawaoka, Yoshihiro, Hebner, Christy, Purcell, Lisa A., Piccoli, Luca, Pizzuto, Matteo Samuele, Walls, Alexandra C., Diamond, Michael S., Telenti, Amalio, Virgin, Herbert W., Lanzavecchia, Antonio, Snell, Gyorgy, Veesler, David, and Corti, Davide

Published
2022
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9. Functional, Chemical, and Phytotoxic Characteristics of Cestrum parqui L'Herit: An Overview.

Author

Di Meo, Maria Chiara, Di Marino, Cinzia, Napoletano, Pasquale, De Marco, Anna, Bianchi, Anna Rita, Pedatella, Silvana, and Palatucci, Domenico

Subjects
SCIENTIFIC knowledge, BIOPESTICIDES, EXTRACTION techniques, METABOLITES, BOTANICAL chemistry
Abstract

Cestrum parqui L'Herit. (Solanaceae family) is a species of forest shrub, self-incompatible and specialized in pollination, widespread in the subtropical area of the planet, and now widely distributed also in the Mediterranean area. The constituents of its leaves have antimicrobial, anticancer, insecticidal, antifeedant, molluscicidal, and herbicidal properties. The spread of this species represents a valuable source of compounds with high biological value. Various research groups are engaged in defining the chemical composition of the different parts of the plant and in defining its properties in view of important and promising commercial applications. To date, there are only a few incomplete reports on the potential applications of C. parqui extracts as selective natural pesticides and on their potential phytotoxic role. Scientific knowledge and the use of extraction techniques for these components are essential for commercial applications. This article summarizes the research and recent studies available on the botany, phytochemistry, functional properties, and commercial applications of C. parqui. [ABSTRACT FROM AUTHOR]

Published
2024
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11. Author Correction: A pan-influenza antibody inhibiting neuraminidase via receptor mimicry

Author

Momont, Corey, Dang, Ha V., Zatta, Fabrizia, Hauser, Kevin, Wang, Caihong, di Iulio, Julia, Minola, Andrea, Czudnochowski, Nadine, De Marco, Anna, Branch, Kaitlin, Donermeyer, David, Vyas, Siddhant, Chen, Alex, Ferri, Elena, Guarino, Barbara, Powell, Abigail E., Spreafico, Roberto, Yim, Samantha S., Balce, Dale R., Bartha, Istvan, Meury, Marcel, Croll, Tristan I., Belnap, David M., Schmid, Michael A., Schaiff, William Timothy, Miller, Jessica L., Cameroni, Elisabetta, Telenti, Amalio, Virgin, Herbert W., Rosen, Laura E., Purcell, Lisa A., Lanzavecchia, Antonio, Snell, Gyorgy, Corti, Davide, and Pizzuto, Matteo Samuele

Published
2023
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12. Broad sarbecovirus neutralization by a human monoclonal antibody

Author

Tortorici, M. Alejandra, Czudnochowski, Nadine, Starr, Tyler N., Marzi, Roberta, Walls, Alexandra C., Zatta, Fabrizia, Bowen, John E., Jaconi, Stefano, Di Iulio, Julia, Wang, Zhaoqian, De Marco, Anna, Zepeda, Samantha K., Pinto, Dora, Liu, Zhuoming, Beltramello, Martina, Bartha, Istvan, Housley, Michael P., Lempp, Florian A., Rosen, Laura E., Dellota, Jr, Exequiel, Kaiser, Hannah, Montiel-Ruiz, Martin, Zhou, Jiayi, Addetia, Amin, Guarino, Barbara, Culap, Katja, Sprugasci, Nicole, Saliba, Christian, Vetti, Eneida, Giacchetto-Sasselli, Isabella, Fregni, Chiara Silacci, Abdelnabi, Rana, Foo, Shi-Yan Caroline, Havenar-Daughton, Colin, Schmid, Michael A., Benigni, Fabio, Cameroni, Elisabetta, Neyts, Johan, Telenti, Amalio, Virgin, Herbert W., Whelan, Sean P. J., Snell, Gyorgy, Bloom, Jesse D., Corti, Davide, Veesler, David, and Pizzuto, Matteo Samuele

Published
2021
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13. Sensitivity of SARS-CoV-2 B.1.1.7 to mRNA vaccine-elicited antibodies

Author

Collier, Dami A., De Marco, Anna, Ferreira, Isabella A. T. M., Meng, Bo, Datir, Rawlings P., Walls, Alexandra C., Kemp, Steven A., Bassi, Jessica, Pinto, Dora, Silacci-Fregni, Chiara, Bianchi, Siro, Tortorici, M. Alejandra, Bowen, John, Culap, Katja, Jaconi, Stefano, Cameroni, Elisabetta, Snell, Gyorgy, Pizzuto, Matteo S., Pellanda, Alessandra Franzetti, Garzoni, Christian, Riva, Agostino, Elmer, Anne, Kingston, Nathalie, Graves, Barbara, McCoy, Laura E., Smith, Kenneth G. C., Bradley, John R., Temperton, Nigel, Ceron-Gutierrez, Lourdes, Barcenas-Morales, Gabriela, Harvey, William, Virgin, Herbert W., Lanzavecchia, Antonio, Piccoli, Luca, Doffinger, Rainer, Wills, Mark, Veesler, David, Corti, Davide, and Gupta, Ravindra K.

Published
2021
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15. Discourse Markers in Italian as L2 in Face to Face vs. Computer Mediated Settings

Author

De Marco, Anna and Leone, Paola

Abstract

This pilot study aims to highlight a) differences in pragmatic function and distribution of discourse markers (DMs) in computer mediated and face to face (FtF) settings and b) any correlation of DM uses and language competence. The data have been collected by video-recording and analysing three speakers of Italian L2 (language level competence: A2, B2) talking with an Italian native speaker face to face and through computer mediated video calls. A pragmatic functional approach has been applied for analysing data. Our investigation shows that the difference between face to face and computer mediated environments is worth noting only in less expert L2 speakers' discourse (i.e. A2 level). In fact, less advanced learners show a tendency to use more discourse markers with an interactional function (specifically addressee oriented) in face to face than in virtual environments. Conversely, there is no remarkable difference in the use of discourse markers by the two more expert speakers. [For full proceedings, see ED565044.]

Published
2013

16. Computer Mediated Conversation for Mutual Learning: Acknowledgement and Agreement/Assessment Signals in Italian as L2

Author

De Marco, Anna and Leone, Paola

Abstract

The current study aims to investigate form, composition, distribution of acknowledgement and agreement/assessment signals in upper intermediate/advanced Italian as L2 speakers. Data are video-transcribed computer mediated conversations for mutual language learning (Teletandem) during which two female volunteer university students (L1: English and German) talk with a native speaker (NS) female interlocutor (L1: Italian) for developing L2 language proficiency. For each pair, data have been collected during two different meetings: meeting 1 comprises free discussion for mutual introductions; meeting 2 is a discussion on a topic chosen by the Italian non-native speaker. The analysis shows that L2 subjects employ a great variety of (1) acknowledgement and agreement listener responses; (2) both lexical and non-lexical units whose functions, positions and frequency are affected by the level of involvement in the discourse; and (3) acquaintance of the two speakers and by the type of task. Particularly in the second meeting, when a topic is discussed, non-native speakers (NNSs) use more lexical units than in the first meeting, thus highlighting the relevance of situational variables over the discourse structure. [For the complete volume, see ED574893.]

Published
2012

17. Author Correction: Sensitivity of SARS-CoV-2 B.1.1.7 to mRNA vaccine-elicited antibodies

Author

Collier, Dami A., De Marco, Anna, Ferreira, Isabella A. T. M., Meng, Bo, Datir, Rawlings P., Walls, Alexandra C., Kemp, Steven A., Bassi, Jessica, Pinto, Dora, Silacci-Fregni, Chiara, Bianchi, Siro, Tortorici, M. Alejandra, Bowen, John, Culap, Katja, Jaconi, Stefano, Cameroni, Elisabetta, Snell, Gyorgy, Pizzuto, Matteo S., Pellanda, Alessandra Franzetti, Garzoni, Christian, Riva, Agostino, Elmer, Anne, Kingston, Nathalie, Graves, Barbara, McCoy, Laura E., Smith, Kenneth G. C., Bradley, John R., Temperton, Nigel, Ceron-Gutierrez, Lourdes, Barcenas-Morales, Gabriela, Harvey, William, Virgin, Herbert W., Lanzavecchia, Antonio, Piccoli, Luca, Doffinger, Rainer, Wills, Mark, Veesler, David, Corti, Davide, and Gupta, Ravindra K.

Published
2022
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18. Cross-neutralization of SARS-CoV-2 by a human monoclonal SARS-CoV antibody

Author

Pinto, Dora, Park, Young-Jun, Beltramello, Martina, Walls, Alexandra C., Tortorici, M. Alejandra, Bianchi, Siro, Jaconi, Stefano, Culap, Katja, Zatta, Fabrizia, De Marco, Anna, Peter, Alessia, Guarino, Barbara, Spreafico, Roberto, Cameroni, Elisabetta, Case, James Brett, Chen, Rita E., Havenar-Daughton, Colin, Snell, Gyorgy, Telenti, Amalio, Virgin, Herbert W., Lanzavecchia, Antonio, Diamond, Michael S., Fink, Katja, Veesler, David, and Corti, Davide

Published
2020
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19. Non-Lethal Assessment of Land Use Change Effects in Water and Soil of Algerian Riparian Areas along the Medjerda River through the Biosentinel Bufo spinosus Daudin.

Author

Napoletano, Pasquale, Guezgouz, Noureddine, Benradia, Imen, Benredjem, Sarra, Parisi, Costantino, Guerriero, Giulia, and De Marco, Anna

Subjects
RIPARIAN areas, LAND use, SOIL moisture, COPPER, SKIN biopsy, AGRICULTURE
Abstract

The land use change can negatively impact ecosystems, enriching water and soil with heavy metals (HMs). The fragile riparian areas along the Medjerda River of Northern Algeria are particularly affected by this phenomenon, and biological tools able to combine information about both matrices can be preferred in their monitoring. This research aimed to evaluate the suitability of the spiny toad (Bufo spinosus D.) as a biomonitor for assessing the impact of three different land uses (agricultural (AGR), urban (URB), and industrial (IND) managements) on soil and water for Cu, Fe, Pb, and Zn by using the non-lethal skin biopsy. The IND land use, followed by URB, mostly impacted soils for Cu and Pb, whereas management was not able to influence water differently despite worrying Pb levels. The cluster analysis allows to show that toad responds like soil in terms of land use and like water for HMs, as it is possibly related to the metal–chemical affinity. Although the single skin analyses do not display any difference among the managements, the bioaccumulation factor (BF > 1) shows that B. spinosus absorbs more HMs from water; skin accumulates Fe and Zn regardless of the land use, probably for both natural and anthropogenic assimilation and Pb and Cu for URB and IND, as it is related to their ionic forms and human impact. [ABSTRACT FROM AUTHOR]

Published
2024
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20. 'NOI ITALIANI SIAMO COSÌ': REALIZZARE L’IDENTITÀ IN UNA CONVERSAZIONE DI EMIGRATI ITALIANI

Author

De Marco, Anna and Palumbo, Mariagrazia

Abstract

Una delle questioni principali nel campo della linguistica migrazionale è senza dubbio la costruzione dell’identità intesa come la costruzione linguistica dell’appartenenza ad uno o più gruppi sociali o categorie, costruita attraverso determinate pratiche linguistiche. La nozione d’identità è considerata un costrutto flessibile: la stessa persona può mettere in scena identità multiple in dipendenza dai diversi contesti d’interazione. La presente ricerca si concentra sul modo in cui le identità sono costruite e in particolare sulle strategie usate dai parlanti per rappresentare le proprie identità. I dati sono tratti dalle conversazioni di sette emigranti calabresi e mostrano come gli informanti indessicalizzano la loro identità come appartenente alla propria cultura o alla cultura del paese d’arrivo. “Noi italiani siamo così”: realising identity in Italian migrants’ conversations One of the main issues in the field of migration linguistics is the construction of identity within discursive practices where speakers call their identities into question starting from the language. Indeed, identity is intended as the linguistic construction of the membership to one or more social groups or categories that are constructed through the use of communicative practices. We assume the notion of identity as a flexible construct: the same person can enact multiple identities depending on the different contexts of interaction. Our study focuses on the way identities are built and used in an encounter and, in particular, on the following research questions: Which strategies do speakers use to represent their identity? Which kind of identity do they perform? Data are taken from the conversations of seven Calabrian emigrants. Our data show that informants alternatively classify themselves as part of their own culture or the culture of the host country, through the stories they tell, showing a plurality of emerging and conflictual identities.

Published
2023

23. Indirect effects of trophic interactions govern carbon circulation in two beech forest soil ecosystems

Author

Lozano Fondon, Carlos, Scotti, Marco, Innangi, Michele, Bondavalli, Cristina, De Marco, Anna, Virzo De Santo, Amalia, Fioretto, Antonietta, Menta, Cristina, Bodini, Antonio, Lozano Fondon, Carlos, Scotti, Marco, Innangi, Michele, Bondavalli, Cristina, De Marco, Anna, Virzo De Santo, Amalia, Fioretto, Antonietta, Menta, Cristina, and Bodini, Antonio

Abstract

Forest soils harbour a vast array of organisms that govern the processing of organic matter. Through their trophic interactions, they give rise to carbon flows that sustain soil ecosystem functioning. Understanding how soil food webs shape carbon flows may enhance our knowledge about the role of biodiversity on soil processes. In this work, we assembled trophic networks representing soil food webs of two beech forests during spring and autumn, and compiled mass-balanced models quantifying carbon flows between their components. These models were investigated using network analysis to identify the role of the components on carbon flow, cycling and functional trophic relationships. Moreover, we explored how the structure of carbon exchanges promote efficiency and stability. Results indicate the importance of indirect interactions. Most trophic groups exhibit a diffuse dependency on all the compartments for their carbon requirement although certain groups such as Collembola play the role of hubs in distributing carbon. Indirect interactions often reverse the impacts of direct trophic relationships being antagonistic to the direction of change predicted based on predator–prey interactions. The high incidence of generalist feeding habits increases the redundancy of energy channels thereby making such food webs more resilient against perturbations but at the expense of carbon transfer efficiency. Although differences can be observed across sites and periods, food web structure rather than environmental variability seems to be the main factor responsible for patterns of carbon flows in the two beech forests. Read the free Plain Language Summary for this article on the Journal blog.

Published
2023
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29. Metadiscursive markers in the context of Italian migration in Germany: an intergenerational perspective

Author

De Marco, Anna, Palumbo, Mariagrazia, De Marco, Anna, and Palumbo, Mariagrazia

Abstract

he present study investigates the use of Discourse Markers (DMs) in the context of Italian migration varieties using an onomasiological approach, i.e. looking at the functions that DMs entail in conversation. Thus, this study focuses on intergenerational variation, namely, how the use of DMs in terms of their functional space varies across generations. We will consider only the metadiscoursive functions, i.e. all the microfunctions aiming at structuring the discourse, ensuring clarity and affecting the organization of discourse. They are analysed in two generations of Italian migrants living in Munich. Data are taken from a corpus of audio recorded interview with 13 Italian migrants living in Munich (average length of each interview, 30 minutes). Results point out that the second-generation informants DMs undergo both a gradual loss of types and a gradual reduction of tokens.

Published
2022

30. Imprinted antibody responses against SARS-CoV-2 Omicron sublineages

Author

Park, Young-Jun, Pinto, Dora, Walls, Alexandra C, Liu, Zhuoming, De Marco, Anna, Benigni, Fabio, Zatta, Fabrizia, Silacci-Fregni, Chiara, Bassi, Jessica, Sprouse, Kaitlin R, Addetia, Amin, Bowen, John E, Stewart, Cameron, Giurdanella, Martina, Saliba, Christian, Guarino, Barbara, Schmid, Michael A, Franko, Nicholas M, Logue, Jennifer K, Dang, Ha V, Hauser, Kevin, di Iulio, Julia, Rivera, William, Schnell, Gretja, Rajesh, Anushka, Zhou, Jiayi, Farhat, Nisar, Kaiser, Hannah, Montiel-Ruiz, Martin, Noack, Julia, et al, Ferrari, Paolo, Ceschi, Alessandro; https://orcid.org/0000-0002-4308-0405, Giannini, Olivier, De Melo, Guilherme Dias, Lanzavecchia, Antonio, Piccoli, Luca, Pizzuto, Matteo Samuele, Corti, Davide; https://orcid.org/0000-0002-5797-1364, Park, Young-Jun, Pinto, Dora, Walls, Alexandra C, Liu, Zhuoming, De Marco, Anna, Benigni, Fabio, Zatta, Fabrizia, Silacci-Fregni, Chiara, Bassi, Jessica, Sprouse, Kaitlin R, Addetia, Amin, Bowen, John E, Stewart, Cameron, Giurdanella, Martina, Saliba, Christian, Guarino, Barbara, Schmid, Michael A, Franko, Nicholas M, Logue, Jennifer K, Dang, Ha V, Hauser, Kevin, di Iulio, Julia, Rivera, William, Schnell, Gretja, Rajesh, Anushka, Zhou, Jiayi, Farhat, Nisar, Kaiser, Hannah, Montiel-Ruiz, Martin, Noack, Julia, et al, Ferrari, Paolo, Ceschi, Alessandro; https://orcid.org/0000-0002-4308-0405, Giannini, Olivier, De Melo, Guilherme Dias, Lanzavecchia, Antonio, Piccoli, Luca, Pizzuto, Matteo Samuele, and Corti, Davide; https://orcid.org/0000-0002-5797-1364

Abstract

SARS-CoV-2 Omicron sublineages carry distinct spike mutations and represent an antigenic shift resulting in escape from antibodies induced by previous infection or vaccination. We show that hybrid immunity or vaccine boosters result in potent plasma neutralizing activity against Omicron BA.1 and BA.2 and that breakthrough infections, but not vaccination-only, induce neutralizing activity in the nasal mucosa. Consistent with immunological imprinting, most antibodies derived from memory B cells or plasma cells of Omicron breakthrough cases cross-react with the Wuhan-Hu-1, BA.1 and BA.2 receptor-binding domains whereas Omicron primary infections elicit B cells of narrow specificity. While most clinical antibodies have reduced neutralization of Omicron, we identified an ultrapotent pan-variant antibody, that is unaffected by any Omicron lineage spike mutations and is a strong candidate for clinical development.

Published
2022

31. Imprinted antibody responses against SARS-CoV-2 Omicron sublineages

Author

Park, Young-Jun; https://orcid.org/0000-0003-2901-6949, Pinto, Dora, Walls, Alexandra C; https://orcid.org/0000-0002-9636-8330, Liu, Zhuoming; https://orcid.org/0000-0001-8198-0976, De Marco, Anna; https://orcid.org/0000-0001-9641-4838, Benigni, Fabio; https://orcid.org/0000-0002-1974-5606, Zatta, Fabrizia; https://orcid.org/0000-0003-4945-8058, Silacci-Fregni, Chiara, Bassi, Jessica; https://orcid.org/0000-0002-4723-6892, Sprouse, Kaitlin R; https://orcid.org/0000-0003-0007-2226, Addetia, Amin; https://orcid.org/0000-0001-6703-3841, Bowen, John E; https://orcid.org/0000-0003-3590-9727, Stewart, Cameron; https://orcid.org/0000-0002-2786-6256, Giurdanella, Martina, Saliba, Christian; https://orcid.org/0000-0003-0100-6976, Guarino, Barbara; https://orcid.org/0000-0003-3759-3252, Schmid, Michael A; https://orcid.org/0000-0002-1137-9322, Franko, Nicholas M; https://orcid.org/0000-0001-8165-6332, Logue, Jennifer K; https://orcid.org/0000-0002-7889-8960, Dang, Ha V; https://orcid.org/0000-0003-1173-9241, Hauser, Kevin, di Iulio, Julia; https://orcid.org/0000-0001-9343-127X, Rivera, William, Schnell, Gretja, Rajesh, Anushka, Zhou, Jiayi; https://orcid.org/0000-0002-4231-3422, Farhat, Nisar; https://orcid.org/0000-0003-0905-7479, Kaiser, Hannah; https://orcid.org/0000-0002-3991-7401, Montiel-Ruiz, Martin; https://orcid.org/0000-0001-6200-9578, Noack, Julia; https://orcid.org/0000-0002-9113-8181, et al, Ferrari, Paolo, Ceschi, Alessandro; https://orcid.org/0000-0002-4308-0405, Giannini, Olivier, de Melo, Guilherme Dias, Lanzavecchia, Antonio, Piccoli, Luca, Pizzuto, Matteo Samuele, Corti, Davide; https://orcid.org/0000-0002-5797-1364, Park, Young-Jun; https://orcid.org/0000-0003-2901-6949, Pinto, Dora, Walls, Alexandra C; https://orcid.org/0000-0002-9636-8330, Liu, Zhuoming; https://orcid.org/0000-0001-8198-0976, De Marco, Anna; https://orcid.org/0000-0001-9641-4838, Benigni, Fabio; https://orcid.org/0000-0002-1974-5606, Zatta, Fabrizia; https://orcid.org/0000-0003-4945-8058, Silacci-Fregni, Chiara, Bassi, Jessica; https://orcid.org/0000-0002-4723-6892, Sprouse, Kaitlin R; https://orcid.org/0000-0003-0007-2226, Addetia, Amin; https://orcid.org/0000-0001-6703-3841, Bowen, John E; https://orcid.org/0000-0003-3590-9727, Stewart, Cameron; https://orcid.org/0000-0002-2786-6256, Giurdanella, Martina, Saliba, Christian; https://orcid.org/0000-0003-0100-6976, Guarino, Barbara; https://orcid.org/0000-0003-3759-3252, Schmid, Michael A; https://orcid.org/0000-0002-1137-9322, Franko, Nicholas M; https://orcid.org/0000-0001-8165-6332, Logue, Jennifer K; https://orcid.org/0000-0002-7889-8960, Dang, Ha V; https://orcid.org/0000-0003-1173-9241, Hauser, Kevin, di Iulio, Julia; https://orcid.org/0000-0001-9343-127X, Rivera, William, Schnell, Gretja, Rajesh, Anushka, Zhou, Jiayi; https://orcid.org/0000-0002-4231-3422, Farhat, Nisar; https://orcid.org/0000-0003-0905-7479, Kaiser, Hannah; https://orcid.org/0000-0002-3991-7401, Montiel-Ruiz, Martin; https://orcid.org/0000-0001-6200-9578, Noack, Julia; https://orcid.org/0000-0002-9113-8181, et al, Ferrari, Paolo, Ceschi, Alessandro; https://orcid.org/0000-0002-4308-0405, Giannini, Olivier, de Melo, Guilherme Dias, Lanzavecchia, Antonio, Piccoli, Luca, Pizzuto, Matteo Samuele, and Corti, Davide; https://orcid.org/0000-0002-5797-1364

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron sublineages carry distinct spike mutations resulting in escape from antibodies induced by previous infection or vaccination. We show that hybrid immunity or vaccine boosters elicit plasma-neutralizing antibodies against Omicron BA.1, BA.2, BA.2.12.1, and BA.4/5, and that breakthrough infections, but not vaccination alone, induce neutralizing antibodies in the nasal mucosa. Consistent with immunological imprinting, most antibodies derived from memory B cells or plasma cells of Omicron breakthrough cases cross-react with the Wuhan-Hu-1, BA.1, BA.2, and BA.4/5 receptor-binding domains, whereas Omicron primary infections elicit B cells of narrow specificity up to 6 months after infection. Although most clinical antibodies have reduced neutralization of Omicron, we identified an ultrapotent pan-variant–neutralizing antibody that is a strong candidate for clinical development.

Published
2022

35. Broadly neutralizing antibodies overcome SARS-CoV-2 Omicron antigenic shift

Author

Cameroni, Elisabetta, primary, Bowen, John E., additional, Rosen, Laura E., additional, Saliba, Christian, additional, Zepeda, Samantha K., additional, Culap, Katja, additional, Pinto, Dora, additional, VanBlargan, Laura A., additional, De Marco, Anna, additional, di Iulio, Julia, additional, Zatta, Fabrizia, additional, Kaiser, Hannah, additional, Noack, Julia, additional, Farhat, Nisar, additional, Czudnochowski, Nadine, additional, Havenar-Daughton, Colin, additional, Sprouse, Kaitlin R., additional, Dillen, Josh R., additional, Powell, Abigail E., additional, Chen, Alex, additional, Maher, Cyrus, additional, Yin, Li, additional, Sun, David, additional, Soriaga, Leah, additional, Bassi, Jessica, additional, Silacci-Fregni, Chiara, additional, Gustafsson, Claes, additional, Franko, Nicholas M., additional, Logue, Jenni, additional, Iqbal, Najeeha Talat, additional, Mazzitelli, Ignacio, additional, Geffner, Jorge, additional, Grifantini, Renata, additional, Chu, Helen, additional, Gori, Andrea, additional, Riva, Agostino, additional, Giannini, Olivier, additional, Ceschi, Alessandro, additional, Ferrari, Paolo, additional, Cippà, Pietro E., additional, Franzetti-Pellanda, Alessandra, additional, Garzoni, Christian, additional, Halfmann, Peter J., additional, Kawaoka, Yoshihiro, additional, Hebner, Christy, additional, Purcell, Lisa A., additional, Piccoli, Luca, additional, Pizzuto, Matteo Samuele, additional, Walls, Alexandra C., additional, Diamond, Michael S., additional, Telenti, Amalio, additional, Virgin, Herbert W., additional, Lanzavecchia, Antonio, additional, Snell, Gyorgy, additional, Veesler, David, additional, and Corti, Davide, additional

Published
2021
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36. Recurrent emergence of SARS-CoV-2 spike deletion H69/V70 and its role in the Alpha variant B.1.1.7

Author

Meng, Bo, Kemp, Steven A, Papa, Guido, Datir, Rawlings, Ferreira, Isabella ATM, Marelli, Sara, Harvey, William T, Lytras, Spyros, Mohamed, Ahmed, Gallo, Giulia, Thakur, Nazia, Collier, Dami A, Mlcochova, Petra, COVID-19 Genomics UK (COG-UK) Consortium, Duncan, Lidia M, Carabelli, Alessandro M, Kenyon, Julia C, Lever, Andrew M, De Marco, Anna, Saliba, Christian, Culap, Katja, Cameroni, Elisabetta, Matheson, Nicholas J, Piccoli, Luca, Corti, Davide, James, Leo C, Robertson, David L, Bailey, Dalan, Gupta, Ravindra K, Kemp, Steven [0000-0001-7077-6793], Datir, Rawlings [0000-0003-0521-2144], Mlcochova, Petra [0000-0001-6908-9363], Carabelli, Alessandro [0000-0003-3625-4021], Matheson, Nicholas [0000-0002-3318-1851], Gupta, Ravindra [0000-0001-9751-1808], and Apollo - University of Cambridge Repository

Subjects
Medical Physiology, Antibodies, Viral, Antibodies, Cell Line, resistance, COVID-19 Genomics UK (COG-UK) Consortium, Recurrence, spike mutation, Chlorocebus aethiops, Animals, Humans, neutralizing antibodies, Viral, deletion, B.1.1.7, Neutralizing, Lung, Pandemics, Vero Cells, Phylogeny, Immune Evasion, SARS-CoV-2, infectivity, COVID-19, Pneumonia, Antibodies, Neutralizing, Spike Glycoprotein, Coronavirus, body regions, Infectious Diseases, Emerging Infectious Diseases, Good Health and Well Being, HEK293 Cells, Alpha variant, Mutation, Spike Glycoprotein, Coronavirus, antibody escape, Biochemistry and Cell Biology, Protein Binding
Abstract

We report severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike ΔH69/V70 in multiple independent lineages, often occurring after acquisition of receptor binding motif replacements such as N439K and Y453F, known to increase binding affinity to the ACE2 receptor and confer antibody escape. Invitro, we show that, although ΔH69/V70 itself is not an antibody evasion mechanism, it increases infectivity associated with enhanced incorporation of cleaved spike into virions. ΔH69/V70 is able to partially rescue infectivity of spike proteins that have acquired N439K and Y453F escape mutations by increased spike incorporation. In addition, replacement of the H69 and V70 residues in the Alpha variant B.1.1.7 spike (where ΔH69/V70 occurs naturally) impairs spike incorporation and entry efficiency of the B.1.1.7 spike pseudotyped virus. Alpha variant B.1.1.7 spike mediates faster kinetics of cell-cell fusion than wild-type Wuhan-1 D614G, dependent on ΔH69/V70. Therefore, as ΔH69/V70 compensates for immune escape mutations that impair infectivity, continued surveillance for deletions with functional effects is warranted.

Published
2021

37. Recurrent emergence of SARS-CoV-2 spike deletion H69/V70 and its role in the variant of concern lineage B.1.1.7

Author

Meng, Bo, Kemp, Steven A., Papa, Guido, Datir, Rawlings, Ferreira, Isabella ATM., Marelli, Sara, Harvey, William T., Lytras, Spyros, Mohamed, Ahmed, Gallo, Giulia, Thakur, Nazia, Collier, Dami A., Mlcochova, Petra, Duncan, Lidia M., Carabelli, Alessandro M., Kenyon, Julia C., Lever, Andrew M., De Marco, Anna, Saliba, Christian, Culap, Katja, Cameroni, Elisabetta, Matheson, Nicholas J., Piccoli, Luca, Corti, Davide, James, Leo C., Robertson, David L., Bailey, Dalan, and Gupta, Ravindra K.

Subjects
body regions
Abstract

We report SARS-CoV-2 spike ΔH69/V70 in multiple independent lineages, often occurring after acquisition of the receptor binding motif replacements such as N439K and Y453F known to increase binding affinity to the ACE2 receptor and confer antibody escape. In vitro, we show that whilst ΔH69/V70 itself is not an antibody evasion mechanism, it increases infectivity associated with enhanced incorporation of cleaved spike into virions. ΔH69/V70 is able to partially rescue infectivity of S proteins that have acquired N439K and Y453F escape mutations by increased spike incorporation. In addition, replacement of H69 and V70 residues in B.1.1.7 spike (where ΔH69/V70 naturally occurs) impairs spike incorporation and entry efficiency of B.1.1.7 spike pseudotyped virus. B.1.1.7 spike mediates faster kinetics of cell-cell fusion than wild type Wuhan-1 D614G, dependent on ΔH69/V70. Therefore, as ΔH69/V70 compensates for immune escape mutations that impair infectivity, continued surveillance for deletions with functional effects is warranted.

Published
2021

41. Recurrent emergence of SARS-CoV-2 spike deletion H69/V70 and its role in the Alpha variant B.1.1.7

Author

Meng, Bo, primary, Kemp, Steven A., additional, Papa, Guido, additional, Datir, Rawlings, additional, Ferreira, Isabella A.T.M., additional, Marelli, Sara, additional, Harvey, William T., additional, Lytras, Spyros, additional, Mohamed, Ahmed, additional, Gallo, Giulia, additional, Thakur, Nazia, additional, Collier, Dami A., additional, Mlcochova, Petra, additional, Duncan, Lidia M., additional, Carabelli, Alessandro M., additional, Kenyon, Julia C., additional, Lever, Andrew M., additional, De Marco, Anna, additional, Saliba, Christian, additional, Culap, Katja, additional, Cameroni, Elisabetta, additional, Matheson, Nicholas J., additional, Piccoli, Luca, additional, Corti, Davide, additional, James, Leo C., additional, Robertson, David L., additional, Bailey, Dalan, additional, Gupta, Ravindra K., additional, Robson, Samuel C., additional, Loman, Nicholas J., additional, Connor, Thomas R., additional, Golubchik, Tanya, additional, Martinez Nunez, Rocio T., additional, Ludden, Catherine, additional, Corden, Sally, additional, Johnston, Ian, additional, Bonsall, David, additional, Smith, Colin P., additional, Awan, Ali R., additional, Bucca, Giselda, additional, Torok, M. Estee, additional, Saeed, Kordo, additional, Prieto, Jacqui A., additional, Jackson, David K., additional, Hamilton, William L., additional, Snell, Luke B., additional, Moore, Catherine, additional, Harrison, Ewan M., additional, Goncalves, Sonia, additional, Fairley, Derek J., additional, Loose, Matthew W., additional, Watkins, Joanne, additional, Livett, Rich, additional, Moses, Samuel, additional, Amato, Roberto, additional, Nicholls, Sam, additional, Bull, Matthew, additional, Smith, Darren L., additional, Barrett, Jeff, additional, Aanensen, David M., additional, Curran, Martin D., additional, Parmar, Surendra, additional, Aggarwal, Dinesh, additional, Shepherd, James G., additional, Parker, Matthew D., additional, Glaysher, Sharon, additional, Bashton, Matthew, additional, Underwood, Anthony P., additional, Pacchiarini, Nicole, additional, Loveson, Katie F., additional, Templeton, Kate E., additional, Langford, Cordelia F., additional, Sillitoe, John, additional, de Silva, Thushan I., additional, Wang, Dennis, additional, Kwiatkowski, Dominic, additional, Rambaut, Andrew, additional, O’Grady, Justin, additional, Cottrell, Simon, additional, Holden, Matthew T.G., additional, Thomson, Emma C., additional, Osman, Husam, additional, Andersson, Monique, additional, Chauhan, Anoop J., additional, Hassan-Ibrahim, Mohammed O., additional, Lawniczak, Mara, additional, Alderton, Alex, additional, Chand, Meera, additional, Constantinidou, Chrystala, additional, Unnikrishnan, Meera, additional, Darby, Alistair C., additional, Hiscox, Julian A., additional, Paterson, Steve, additional, Martincorena, Inigo, additional, Volz, Erik M., additional, Page, Andrew J., additional, Pybus, Oliver G., additional, Bassett, Andrew R., additional, Ariani, Cristina V., additional, Chapman, Michael H. Spencer, additional, Li, Kathy K., additional, Shah, Rajiv N., additional, Jesudason, Natasha G., additional, Taha, Yusri, additional, McHugh, Martin P., additional, Dewar, Rebecca, additional, Jahun, Aminu S., additional, McMurray, Claire, additional, Pandey, Sarojini, additional, McKenna, James P., additional, Nelson, Andrew, additional, Young, Gregory R., additional, McCann, Clare M., additional, Elliott, Scott, additional, Lowe, Hannah, additional, Temperton, Ben, additional, Roy, Sunando, additional, Price, Anna, additional, Rey, Sara, additional, Wyles, Matthew, additional, Rooke, Stefan, additional, Shaaban, Sharif, additional, de Cesare, Mariateresa, additional, Letchford, Laura, additional, Silveira, Siona, additional, Pelosi, Emanuela, additional, Wilson-Davies, Eleri, additional, Hosmillo, Myra, additional, O’Toole, Áine, additional, Hesketh, Andrew R., additional, Stark, Richard, additional, du Plessis, Louis, additional, Ruis, Chris, additional, Adams, Helen, additional, Bourgeois, Yann, additional, Michell, Stephen L., additional, Grammatopoulos, Dimitris, additional, Edgeworth, Jonathan, additional, Breuer, Judith, additional, Todd, John A., additional, Fraser, Christophe, additional, Buck, David, additional, John, Michaela, additional, Kay, Gemma L., additional, Palmer, Steve, additional, Peacock, Sharon J., additional, Heyburn, David, additional, Weldon, Danni, additional, Robinson, Esther, additional, McNally, Alan, additional, Muir, Peter, additional, Vipond, Ian B., additional, Boyes, John, additional, Sivaprakasam, Venkat, additional, Salluja, Tranprit, additional, Dervisevic, Samir, additional, Meader, Emma J., additional, Park, Naomi R., additional, Oliver, Karen, additional, Jeffries, Aaron R., additional, Ott, Sascha, additional, da Silva Filipe, Ana, additional, Simpson, David A., additional, Williams, Chris, additional, Masoli, Jane A.H., additional, Knight, Bridget A., additional, Jones, Christopher R., additional, Koshy, Cherian, additional, Ash, Amy, additional, Casey, Anna, additional, Bosworth, Andrew, additional, Ratcliffe, Liz, additional, Xu-McCrae, Li, additional, Pymont, Hannah M., additional, Hutchings, Stephanie, additional, Berry, Lisa, additional, Jones, Katie, additional, Halstead, Fenella, additional, Davis, Thomas, additional, Holmes, Christopher, additional, Iturriza-Gomara, Miren, additional, Lucaci, Anita O., additional, Randell, Paul Anthony, additional, Cox, Alison, additional, Madona, Pinglawathee, additional, Harris, Kathryn Ann, additional, Brown, Julianne Rose, additional, Mahungu, Tabitha W., additional, Irish-Tavares, Dianne, additional, Haque, Tanzina, additional, Hart, Jennifer, additional, Witele, Eric, additional, Fenton, Melisa Louise, additional, Liggett, Steven, additional, Graham, Clive, additional, Swindells, Emma, additional, Collins, Jennifer, additional, Eltringham, Gary, additional, Campbell, Sharon, additional, McClure, Patrick C., additional, Clark, Gemma, additional, Sloan, Tim J., additional, Jones, Carl, additional, Lynch, Jessica, additional, Warne, Ben, additional, Leonard, Steven, additional, Durham, Jillian, additional, Williams, Thomas, additional, Haldenby, Sam T., additional, Storey, Nathaniel, additional, Alikhan, Nabil-Fareed, additional, Holmes, Nadine, additional, Moore, Christopher, additional, Carlile, Matthew, additional, Perry, Malorie, additional, Craine, Noel, additional, Lyons, Ronan A., additional, Beckett, Angela H., additional, Goudarzi, Salman, additional, Fearn, Christopher, additional, Cook, Kate, additional, Dent, Hannah, additional, Paul, Hannah, additional, Davies, Robert, additional, Blane, Beth, additional, Girgis, Sophia T., additional, Beale, Mathew A., additional, Bellis, Katherine L., additional, Dorman, Matthew J., additional, Drury, Eleanor, additional, Kane, Leanne, additional, Kay, Sally, additional, McGuigan, Samantha, additional, Nelson, Rachel, additional, Prestwood, Liam, additional, Rajatileka, Shavanthi, additional, Batra, Rahul, additional, Williams, Rachel J., additional, Kristiansen, Mark, additional, Green, Angie, additional, Justice, Anita, additional, Mahanama, Adhyana I.K., additional, Samaraweera, Buddhini, additional, Hadjirin, Nazreen F., additional, Quick, Joshua, additional, Poplawski, Radoslaw, additional, Kermack, Leanne M., additional, Reynolds, Nicola, additional, Hall, Grant, additional, Chaudhry, Yasmin, additional, Pinckert, Malte L., additional, Georgana, Iliana, additional, Moll, Robin J., additional, Thornton, Alicia, additional, Myers, Richard, additional, Stockton, Joanne, additional, Williams, Charlotte A., additional, Yew, Wen C., additional, Trotter, Alexander J., additional, Trebes, Amy, additional, MacIntyre-Cockett, George, additional, Birchley, Alec, additional, Adams, Alexander, additional, Plimmer, Amy, additional, Gatica-Wilcox, Bree, additional, McKerr, Caoimhe, additional, Hilvers, Ember, additional, Jones, Hannah, additional, Asad, Hibo, additional, Coombes, Jason, additional, Evans, Johnathan M., additional, Fina, Laia, additional, Gilbert, Lauren, additional, Graham, Lee, additional, Cronin, Michelle, additional, Kumziene-Summerhayes, Sara, additional, Taylor, Sarah, additional, Jones, Sophie, additional, Groves, Danielle C., additional, Zhang, Peijun, additional, Gallis, Marta, additional, Louka, Stavroula F., additional, Starinskij, Igor, additional, Jackson, Chris, additional, Gourtovaia, Marina, additional, Tonkin-Hill, Gerry, additional, Lewis, Kevin, additional, Tovar-Corona, Jaime M., additional, James, Keith, additional, Baxter, Laura, additional, Alam, Mohammad T., additional, Orton, Richard J., additional, Hughes, Joseph, additional, Vattipally, Sreenu, additional, Ragonnet-Cronin, Manon, additional, Nascimento, Fabricia F., additional, Jorgensen, David, additional, Boyd, Olivia, additional, Geidelberg, Lily, additional, Zarebski, Alex E., additional, Raghwani, Jayna, additional, Kraemer, Moritz U.G., additional, Southgate, Joel, additional, Lindsey, Benjamin B., additional, Freeman, Timothy M., additional, Keatley, Jon-Paul, additional, Singer, Joshua B., additional, de Oliveira Martins, Leonardo, additional, Yeats, Corin A., additional, Abudahab, Khalil, additional, Taylor, Ben E.W., additional, Menegazzo, Mirko, additional, Danesh, John, additional, Hogsden, Wendy, additional, Eldirdiri, Sahar, additional, Kenyon, Anita, additional, Mason, Jenifer, additional, Robinson, Trevor I., additional, Holmes, Alison, additional, Price, James, additional, Hartley, John A., additional, Curran, Tanya, additional, Mather, Alison E., additional, Shankar, Giri, additional, Jones, Rachel, additional, Howe, Robin, additional, Morgan, Sian, additional, Wastenge, Elizabeth, additional, Chapman, Michael R., additional, Mookerjee, Siddharth, additional, Stanley, Rachael, additional, Smith, Wendy, additional, Peto, Timothy, additional, Eyre, David, additional, Crook, Derrick, additional, Vernet, Gabrielle, additional, Kitchen, Christine, additional, Gulliver, Huw, additional, Merrick, Ian, additional, Guest, Martyn, additional, Munn, Robert, additional, Bradley, Declan T., additional, Wyatt, Tim, additional, Beaver, Charlotte, additional, Foulser, Luke, additional, Palmer, Sophie, additional, Churcher, Carol M., additional, Brooks, Ellena, additional, Smith, Kim S., additional, Galai, Katerina, additional, McManus, Georgina M., additional, Bolt, Frances, additional, Coll, Francesc, additional, Meadows, Lizzie, additional, Attwood, Stephen W., additional, Davies, Alisha, additional, De Lacy, Elen, additional, Downing, Fatima, additional, Edwards, Sue, additional, Scarlett, Garry P., additional, Jeremiah, Sarah, additional, Smith, Nikki, additional, Leek, Danielle, additional, Sridhar, Sushmita, additional, Forrest, Sally, additional, Cormie, Claire, additional, Gill, Harmeet K., additional, Dias, Joana, additional, Higginson, Ellen E., additional, Maes, Mailis, additional, Young, Jamie, additional, Wantoch, Michelle, additional, Jamrozy, Dorota, additional, Lo, Stephanie, additional, Patel, Minal, additional, Hill, Verity, additional, Bewshea, Claire M., additional, Ellard, Sian, additional, Auckland, Cressida, additional, Harrison, Ian, additional, Bishop, Chloe, additional, Chalker, Vicki, additional, Richter, Alex, additional, Beggs, Andrew, additional, Best, Angus, additional, Percival, Benita, additional, Mirza, Jeremy, additional, Megram, Oliver, additional, Mayhew, Megan, additional, Crawford, Liam, additional, Ashcroft, Fiona, additional, Moles-Garcia, Emma, additional, Cumley, Nicola, additional, Hopes, Richard, additional, Asamaphan, Patawee, additional, Niebel, Marc O., additional, Gunson, Rory N., additional, Bradley, Amanda, additional, Maclean, Alasdair, additional, Mollett, Guy, additional, Blacow, Rachel, additional, Bird, Paul, additional, Helmer, Thomas, additional, Fallon, Karlie, additional, Tang, Julian, additional, Hale, Antony D., additional, Macfarlane-Smith, Louissa R., additional, Harper, Katherine L., additional, Carden, Holli, additional, Machin, Nicholas W., additional, Jackson, Kathryn A., additional, Ahmad, Shazaad S.Y., additional, George, Ryan P., additional, Turtle, Lance, additional, O’Toole, Elaine, additional, Watts, Joanne, additional, Breen, Cassie, additional, Cowell, Angela, additional, Alcolea-Medina, Adela, additional, Charalampous, Themoula, additional, Patel, Amita, additional, Levett, Lisa J., additional, Heaney, Judith, additional, Rowan, Aileen, additional, Taylor, Graham P., additional, Shah, Divya, additional, Atkinson, Laura, additional, Lee, Jack C.D., additional, Westhorpe, Adam P., additional, Jannoo, Riaz, additional, Lowe, Helen L., additional, Karamani, Angeliki, additional, Ensell, Leah, additional, Chatterton, Wendy, additional, Pusok, Monika, additional, Dadrah, Ashok, additional, Symmonds, Amanda, additional, Sluga, Graciela, additional, Molnar, Zoltan, additional, Baker, Paul, additional, Bonner, Stephen, additional, Essex, Sarah, additional, Barton, Edward, additional, Padgett, Debra, additional, Scott, Garren, additional, Greenaway, Jane, additional, Payne, Brendan A.I., additional, Burton-Fanning, Shirelle, additional, Waugh, Sheila, additional, Raviprakash, Veena, additional, Sheriff, Nicola, additional, Blakey, Victoria, additional, Williams, Lesley-Anne, additional, Moore, Jonathan, additional, Stonehouse, Susanne, additional, Smith, Louise, additional, Davidson, Rose K., additional, Bedford, Luke, additional, Coupland, Lindsay, additional, Wright, Victoria, additional, Chappell, Joseph G., additional, Tsoleridis, Theocharis, additional, Ball, Jonathan, additional, Khakh, Manjinder, additional, Fleming, Vicki M., additional, Lister, Michelle M., additional, Howson-Wells, Hannah C., additional, Berry, Louise, additional, Boswell, Tim, additional, Joseph, Amelia, additional, Willingham, Iona, additional, Duckworth, Nichola, additional, Walsh, Sarah, additional, Wise, Emma, additional, Moore, Nathan, additional, Mori, Matilde, additional, Cortes, Nick, additional, Kidd, Stephen, additional, Williams, Rebecca, additional, Gifford, Laura, additional, Bicknell, Kelly, additional, Wyllie, Sarah, additional, Lloyd, Allyson, additional, Impey, Robert, additional, Malone, Cassandra S., additional, Cogger, Benjamin J., additional, Levene, Nick, additional, Monaghan, Lynn, additional, Keeley, Alexander J., additional, Partridge, David G., additional, Raza, Mohammad, additional, Evans, Cariad, additional, Johnson, Kate, additional, Betteridge, Emma, additional, Farr, Ben W., additional, Goodwin, Scott, additional, Quail, Michael A., additional, Scott, Carol, additional, Shirley, Lesley, additional, Thurston, Scott A.J., additional, Rajan, Diana, additional, Bronner, Iraad F., additional, Aigrain, Louise, additional, Redshaw, Nicholas M., additional, Lensing, Stefanie V., additional, McCarthy, Shane, additional, Makunin, Alex, additional, Balcazar, Carlos E., additional, Gallagher, Michael D., additional, Williamson, Kathleen A., additional, Stanton, Thomas D., additional, Michelsen, Michelle L., additional, Warwick-Dugdale, Joanna, additional, Manley, Robin, additional, Farbos, Audrey, additional, Harrison, James W., additional, Sambles, Christine M., additional, Studholme, David J., additional, Lackenby, Angie, additional, Mbisa, Tamyo, additional, Platt, Steven, additional, Miah, Shahjahan, additional, Bibby, David, additional, Manso, Carmen, additional, Hubb, Jonathan, additional, Dabrera, Gavin, additional, Ramsay, Mary, additional, Bradshaw, Daniel, additional, Schaefer, Ulf, additional, Groves, Natalie, additional, Gallagher, Eileen, additional, Lee, David, additional, Williams, David, additional, Ellaby, Nicholas, additional, Hartman, Hassan, additional, Manesis, Nikos, additional, Patel, Vineet, additional, Ledesma, Juan, additional, Twohig, Katherine A., additional, Allara, Elias, additional, Pearson, Clare, additional, Cheng, Jeffrey K.J., additional, Bridgewater, Hannah E., additional, Frost, Lucy R., additional, Taylor-Joyce, Grace, additional, Brown, Paul E., additional, Tong, Lily, additional, Broos, Alice, additional, Mair, Daniel, additional, Nichols, Jenna, additional, Carmichael, Stephen N., additional, Smollett, Katherine L., additional, Nomikou, Kyriaki, additional, Aranday-Cortes, Elihu, additional, Johnson, Natasha, additional, Nickbakhsh, Seema, additional, Vamos, Edith E., additional, Hughes, Margaret, additional, Rainbow, Lucille, additional, Eccles, Richard, additional, Nelson, Charlotte, additional, Whitehead, Mark, additional, Gregory, Richard, additional, Gemmell, Matthew, additional, Wierzbicki, Claudia, additional, Webster, Hermione J., additional, Fisher, Chloe L., additional, Signell, Adrian W., additional, Betancor, Gilberto, additional, Wilson, Harry D., additional, Nebbia, Gaia, additional, Flaviani, Flavia, additional, Cerda, Alberto C., additional, Merrill, Tammy V., additional, Wilson, Rebekah E., additional, Cotic, Marius, additional, Bayzid, Nadua, additional, Thompson, Thomas, additional, Acheson, Erwan, additional, Rushton, Steven, additional, O’Brien, Sarah, additional, Baker, David J., additional, Rudder, Steven, additional, Aydin, Alp, additional, Sang, Fei, additional, Debebe, Johnny, additional, Francois, Sarah, additional, Vasylyeva, Tetyana I., additional, Zamudio, Marina Escalera, additional, Gutierrez, Bernardo, additional, Marchbank, Angela, additional, Maksimovic, Joshua, additional, Spellman, Karla, additional, McCluggage, Kathryn, additional, Morgan, Mari, additional, Beer, Robert, additional, Afifi, Safiah, additional, Workman, Trudy, additional, Fuller, William, additional, Bresner, Catherine, additional, Angyal, Adrienn, additional, Green, Luke R., additional, Parsons, Paul J., additional, Tucker, Rachel M., additional, Brown, Rebecca, additional, Whiteley, Max, additional, Bonfield, James, additional, Puethe, Christoph, additional, Whitwham, Andrew, additional, Liddle, Jennifier, additional, Rowe, Will, additional, Siveroni, Igor, additional, Le-Viet, Thanh, additional, Gaskin, Amy, additional, Johnson, Rob, additional, Abnizova, Irina, additional, Ali, Mozam, additional, Allen, Laura, additional, Anderson, Ralph, additional, Ariani, Cristina, additional, Austin-Guest, Siobhan, additional, Bala, Sendu, additional, Barrett, Jeffrey, additional, Bassett, Andrew, additional, Battleday, Kristina, additional, Beal, James, additional, Beale, Mathew, additional, Bellany, Sam, additional, Bellerby, Tristram, additional, Bellis, Katie, additional, Berger, Duncan, additional, Berriman, Matt, additional, Bevan, Paul, additional, Binley, Simon, additional, Bishop, Jason, additional, Blackburn, Kirsty, additional, Boughton, Nick, additional, Bowker, Sam, additional, Brendler-Spaeth, Timothy, additional, Bronner, Iraad, additional, Brooklyn, Tanya, additional, Buddenborg, Sarah Kay, additional, Bush, Robert, additional, Caetano, Catarina, additional, Cagan, Alex, additional, Carter, Nicola, additional, Cartwright, Joanna, additional, Monteiro, Tiago Carvalho, additional, Chapman, Liz, additional, Chillingworth, Tracey-Jane, additional, Clapham, Peter, additional, Clark, Richard, additional, Clarke, Adrian, additional, Clarke, Catriona, additional, Cole, Daryl, additional, Cook, Elizabeth, additional, Coppola, Maria, additional, Cornell, Linda, additional, Cornwell, Clare, additional, Corton, Craig, additional, Crackett, Abby, additional, Cranage, Alison, additional, Craven, Harriet, additional, Craw, Sarah, additional, Crawford, Mark, additional, Cutts, Tim, additional, Dabrowska, Monika, additional, Davies, Matt, additional, Dawson, Joseph, additional, Day, Callum, additional, Densem, Aiden, additional, Dibling, Thomas, additional, Dockree, Cat, additional, Dodd, David, additional, Dogga, Sunil, additional, Dorman, Matthew, additional, Dougan, Gordon, additional, Dougherty, Martin, additional, Dove, Alexander, additional, Drummond, Lucy, additional, Dudek, Monika, additional, Durrant, Laura, additional, Easthope, Elizabeth, additional, Eckert, Sabine, additional, Ellis, Pete, additional, Farr, Ben, additional, Fenton, Michael, additional, Ferrero, Marcella, additional, Flack, Neil, additional, Fordham, Howerd, additional, Forsythe, Grace, additional, Francis, Matt, additional, Fraser, Audrey, additional, Freeman, Adam, additional, Galvin, Anastasia, additional, Garcia-Casado, Maria, additional, Gedny, Alex, additional, Girgis, Sophia, additional, Glover, James, additional, Gould, Oliver, additional, Gray, Andy, additional, Gray, Emma, additional, Griffiths, Coline, additional, Gu, Yong, additional, Guerin, Florence, additional, Hamilton, Will, additional, Hanks, Hannah, additional, Harrison, Ewan, additional, Harrott, Alexandria, additional, Harry, Edward, additional, Harvison, Julia, additional, Heath, Paul, additional, Hernandez-Koutoucheva, Anastasia, additional, Hobbs, Rhiannon, additional, Holland, Dave, additional, Holmes, Sarah, additional, Hornett, Gary, additional, Hough, Nicholas, additional, Huckle, Liz, additional, Hughes-Hallet, Lena, additional, Hunter, Adam, additional, Inglis, Stephen, additional, Iqbal, Sameena, additional, Jackson, Adam, additional, Jackson, David, additional, Verdejo, Carlos Jimenez, additional, Jones, Matthew, additional, Kallepally, Kalyan, additional, Kay, Keely, additional, Keatley, Jon, additional, Keith, Alan, additional, King, Alison, additional, Kitchin, Lucy, additional, Kleanthous, Matt, additional, Klimekova, Martina, additional, Korlevic, Petra, additional, Krasheninnkova, Ksenia, additional, Lane, Greg, additional, Langford, Cordelia, additional, Laverack, Adam, additional, Law, Katharine, additional, Lensing, Stefanie, additional, Lewis-Wade, Amanah, additional, Liddle, Jennifer, additional, Lin, Quan, additional, Lindsay, Sarah, additional, Linsdell, Sally, additional, Long, Rhona, additional, Lovell, Jamie, additional, Lovell, Jon, additional, Mack, James, additional, Maddison, Mark, additional, Makunin, Aleksei, additional, Mamun, Irfan, additional, Mansfield, Jenny, additional, Marriott, Neil, additional, Martin, Matt, additional, Mayho, Matthew, additional, McClintock, Jo, additional, McHugh, Sandra, additional, MapcMinn, Liz, additional, Meadows, Carl, additional, Mobley, Emily, additional, Moll, Robin, additional, Morra, Maria, additional, Morrow, Leanne, additional, Murie, Kathryn, additional, Nash, Sian, additional, Nathwani, Claire, additional, Naydenova, Plamena, additional, Neaverson, Alexandra, additional, Nerou, Ed, additional, Nicholson, Jon, additional, Nimz, Tabea, additional, Noell, Guillaume G., additional, O’Meara, Sarah, additional, Ohan, Valeriu, additional, Olney, Charles, additional, Ormond, Doug, additional, Oszlanczi, Agnes, additional, Pang, Yoke Fei, additional, Pardubska, Barbora, additional, Park, Naomi, additional, Parmar, Aaron, additional, Patel, Gaurang, additional, Payne, Maggie, additional, Peacock, Sharon, additional, Petersen, Arabella, additional, Plowman, Deborah, additional, Preston, Tom, additional, Quail, Michael, additional, Rance, Richard, additional, Rawlings, Suzannah, additional, Redshaw, Nicholas, additional, Reynolds, Joe, additional, Reynolds, Mark, additional, Rice, Simon, additional, Richardson, Matt, additional, Roberts, Connor, additional, Robinson, Katrina, additional, Robinson, Melanie, additional, Robinson, David, additional, Rogers, Hazel, additional, Rojo, Eduardo Martin, additional, Roopra, Daljit, additional, Rose, Mark, additional, Rudd, Luke, additional, Sadri, Ramin, additional, Salmon, Nicholas, additional, Saul, David, additional, Schwach, Frank, additional, Seekings, Phil, additional, Simms, Alison, additional, Sinnott, Matt, additional, Sivadasan, Shanthi, additional, Siwek, Bart, additional, Sizer, Dale, additional, Skeldon, Kenneth, additional, Skelton, Jason, additional, Slater-Tunstill, Joanna, additional, Sloper, Lisa, additional, Smerdon, Nathalie, additional, Smith, Chris, additional, Smith, Christen, additional, Smith, James, additional, Smith, Katie, additional, Smith, Michelle, additional, Smith, Sean, additional, Smith, Tina, additional, Sneade, Leighton, additional, Soria, Carmen Diaz, additional, Sousa, Catarina, additional, Souster, Emily, additional, Sparkes, Andrew, additional, Spencer-Chapman, Michael, additional, Squares, Janet, additional, Stanley, Robert, additional, Steed, Claire, additional, Stickland, Tim, additional, Still, Ian, additional, Stratton, Mike, additional, Strickland, Michelle, additional, Swann, Allen, additional, Swiatkowska, Agnieszka, additional, Sycamore, Neil, additional, Swift, Emma, additional, Symons, Edward, additional, Szluha, Suzanne, additional, Taluy, Emma, additional, Tao, Nunu, additional, Taylor, Katy, additional, Taylor, Sam, additional, Thompson, Stacey, additional, Thompson, Mark, additional, Thomson, Mark, additional, Thomson, Nicholas, additional, Thurston, Scott, additional, Toombs, Dee, additional, Topping, Benjamin, additional, Tovar-Corona, Jaime, additional, Ungureanu, Daniel, additional, Uphill, James, additional, Urbanova, Jana, additional, Van, Philip Jansen, additional, Vancollie, Valerie, additional, Voak, Paul, additional, Walker, Danielle, additional, Walker, Matthew, additional, Waller, Matt, additional, Ward, Gary, additional, Weatherhogg, Charlie, additional, Webb, Niki, additional, Wells, Alan, additional, Wells, Eloise, additional, Westwood, Luke, additional, Whipp, Theo, additional, Whiteley, Thomas, additional, Whitton, Georgia, additional, Widaa, Sara, additional, Williams, Mia, additional, Wilson, Mark, additional, and Wright, Sean, additional

Published
2021
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42. Combined Effects of Wildfire and Vegetation Cover Type on Volcanic Soil (Functions and Properties) in a Mediterranean Region: Comparison of Two Soil Quality Indices

Author

Santorufo, Lucia, primary, Memoli, Valeria, additional, Panico, Speranza Claudia, additional, Santini, Giorgia, additional, Barile, Rossella, additional, Giarra, Antonella, additional, Di Natale, Gabriella, additional, Trifuoggi, Marco, additional, De Marco, Anna, additional, and Maisto, Giulia, additional

Published
2021
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44. L’INTERAZIONE TRA NATIVO-NON NATIVO E NON NATIVO - NON NATIVO: I SEGNALI DISCORSIVI IN APPRENDENTI ISPANOFONI DI ITALIANO L2

Author

De Marco, Anna

Subjects
lcsh:Language and Literature, lcsh:Philology. Linguistics, lcsh:P1-1091, lcsh:P
Abstract

La ricerca esplora il ruolo dei segnali discorsivi (SD) di tipo interazionale nella dinamica delle conversazioni di apprendenti spagnoli di italiano (di livello di competenza A2) impegnati in due diversi tipi di interazione: nativo-non nativo (asimmetrico) e non nativo-non nativo (simmetrico). L’analisi prende in considerazione due contesti conversazionali: nel primo, gli apprendenti sono impegnati in un’attività più guidata consistente nello scambio di informazioni, nel secondo nella costruzione un dialogo basato su uno scenario predefinito, come quello di decidere un regalo o organizzare una festa per un amico. Nelle conversazioni asimmetriche il dominio dei nativi nel guidare la conversazione dei non nativi emerge dalla presenza di un maggior numero di segnali di accordo e conferma di ricezione da parte degli apprendenti. D’altra parte, nelle interazioni simmetriche, la varietà e la frequenza dei vari SD utilizzati dai non nativi, come la presa di turno, la richiesta di conferma, mostrano un maggiore coinvolgimento degli stessi nell'interazione, soprattutto nel caso delle interazioni più libere (role-play). Native/non-native and non-native/non native interaction: discourse markers in Spanish-speaking learners of Italian L2 This study explores the role of interactional DM in the dynamics of conversations of Spanish native speakers (with A2 level of proficiency) engaged in two different interaction types: native/ non-native (asymmetric) and non-native/non-native (symmetric). Eight learners and two native speakers were involved in two conversational settings: in the first, pairs were engaged in an information exchange activity, in the second they had to construct a dialogue based on a predefined scenario, such as deciding on a gift or organizing a party for a friend. In asymmetrical conversations the dominance of natives in guiding the conversation of non-natives can be shown based on the greater number agreement and acknowledgment signals used by the learners. On the other hand, in symmetrical interactions, the variety and the frequency of various DMs used by non-natives, such as turn taking and requesting confirmation, shows greater involvement in the interaction, especially in role-play situations., Italiano LinguaDue, V. 12 N. 2 (2020)

Published
2021
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45. Shifts in soil chemical and microbial properties across forest chronosequence on recent volcanic deposits

Author

De Marco, Anna, Berg, Björn, Zarrelli, Armando, Virzo De Santo, Amalia, De Marco, Anna, Berg, Björn, Zarrelli, Armando, and Virzo De Santo, Amalia

Abstract

Afforestation of new unconsolidated volcanic deposits is a practice used to stabilize barren areas and enhance the accumulation of organic matter in the developing soil. Changes in soil carbon (C) and nitrogen (N) pools, including the soluble and microbial fractions, within the first decades since afforestation have been poorly investigated. Therefore the objective of the present study was to investigate how key C and N pools vary in litter and soil of four forests planted on barren volcanic deposits from recent Mount Vesuvius eruptions. We examined three forest stands (40, 70 and 100 years old) afforested with Stone pine (Pinus pinea L.) and a 40-year old forest of Black pine (Pinus nigra Arn.). As a baseline of C and N pools prior to afforestation, data from treeless sites were included in the study. Both the inputs with litter fall and soil C and N stocks increased with forest age in the Stone pine stands. In the mineral soil, C concentration per gram soil dry weight and C:N ratio increased with age from treeless sites to the oldest forest. Microbial biomass C and fungal biomass as a fraction of organic carbon (OC) and respiration per unit OC (an index of organic matter mineralization potential) decreased significantly with stand age. The results suggest that a main driver of C accumulation in the mineral soil is the decline with increasing stand age of the microbial fraction of organic matter and its activity. The comparison between the two pine species revealed that litter production was more abundant in the Black pine than in the even-aged, 40-year-old, Stone pine stand; moreover Black pine litter was more acidic and had a higher stable residue than Stone pine litter. Therefore a different pattern of C sequestration occurs with a higher C stock in the organic layers and a lower C stock in the mineral soil of Black pine compared to Stone pine.

Published
2021
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46. Structure and function analysis of an antibody recognizing all influenza A subtypes

Author

Kallewaard, Nicole L., Corti, Davide, Collins, Patrick J., Neu, Ursula, McAuliffe, Josephine M., Benjamin, Ebony, Wachter-Rosati, Leslie, Palmer-Hill, Frances J., Yuan, Andy Q., Walker, Philip A., Vorlaender, Matthias K., Bianchi, Siro, Guarino, Barbara, De Marco, Anna, Vanzetta, Fabrizia, Agatic, Gloria, Foglierini, Mathilde, Pinna, Debora, Fernandez-Rodriguez, Blanca, Fruehwirth, Alexander, Silacci, Chiara, Ogrodowicz, Roksana W., Martin, Stephen R., Sallusto, Federica, Suzich, JoAnn A., Lanzavecchia, Antonio, Zhu, Qing, Gamblin, Steven J., Skehel, John J., Kallewaard, Nicole L., Corti, Davide, Collins, Patrick J., Neu, Ursula, McAuliffe, Josephine M., Benjamin, Ebony, Wachter-Rosati, Leslie, Palmer-Hill, Frances J., Yuan, Andy Q., Walker, Philip A., Vorlaender, Matthias K., Bianchi, Siro, Guarino, Barbara, De Marco, Anna, Vanzetta, Fabrizia, Agatic, Gloria, Foglierini, Mathilde, Pinna, Debora, Fernandez-Rodriguez, Blanca, Fruehwirth, Alexander, Silacci, Chiara, Ogrodowicz, Roksana W., Martin, Stephen R., Sallusto, Federica, Suzich, JoAnn A., Lanzavecchia, Antonio, Zhu, Qing, Gamblin, Steven J., and Skehel, John J.

Abstract

Influenza virus remains a threat because of its ability to evade vaccine-induced immune responses due to antigenic drift. Here, we describe the isolation, evolution, and structure of a broad-spectrum human monoclonal antibody (mAb), MEDI8852, effectively reacting with all influenza A hemagglutinin (HA) subtypes. MEDI8852 uses the heavy-chain VH6-1 gene and has higher potency and breadth when compared to other anti-stem antibodies. MEDI8852 is effective in mice and ferrets with a therapeutic window superior to that of oseltamivir. Crystallographic analysis of Fab alone or in complex with H5 or H7 HA proteins reveals that MEDI8852 binds through a coordinated movement of CDRs to a highly conserved epitope encompassing a hydrophobic groove in the fusion domain and a large portion of the fusion peptide, distinguishing it from other structurally characterized cross- reactive antibodies. The unprecedented breadth and potency of neutralization by MEDI8852 support its development as immunotherapy for influenza virus-infected humans.

Published
2021

47. The circulating SARS-CoV-2 spike variant N439K maintains fitness while evading antibody-mediated immunity

Author

Thomson, Emma C., Rosen, Laura E., Shepherd, James G., Spreafico, Roberto, da Silva Filipe, Ana, Wojcechowskyj, Jason A., Davis, Chris, Piccoli, Luca, Pascall, David J., Dillen, Josh, Lytras, Spyros, Czudnochowski, Nadine, Shah, Rajiv, Meury, Marcel, Jesudason, Natasha, De Marco, Anna, Li, Kathy, Bassi, Jessica, O’Toole, Aine, Pinto, Dora, Colquhoun, Rachel M., Culap, Katja, Jackson, Ben, Zatta, Fabrizia, Rambaut, Andrew, Jaconi, Stefano, Sreenu, Vattipally B., Nix, Jay, Jarrett, Ruth F., Beltramello, Martina, Nomikou, Kyriaki, Pizzuto, Matteo, Tong, Lily, Cameroni, Elisabetta, Johnson, Natasha, Wickenhagen, Arthur, Ceschi, Alessandro, Mair, Daniel, Ferrari, Paolo, Smollett, Katherine, Sallusto, Federica, Carmichael, Stephen, Garzoni, Christian, Nichols, Jenna, Galli, Massimo, Hughes, Joseph, Riva, Agostino, Ho, Antonia, Semple, Malcolm G., Openshaw, Peter J.M., Baillie, J. Kenneth, Rihn, Suzannah J., Lycett, Samantha J., Virgin, Herbert W., Telenti, Amalio, Corti, Davide, Robertson, David L., Snell, Gyorgy, and Fadda, Elisa

Subjects
Immune system, biology, medicine.drug_class, Immunity, Polyclonal antibodies, biology.protein, medicine, Virulence, Antibody, Monoclonal antibody, Receptor, Virology, Virus
Abstract

SARS-CoV-2 can mutate to evade immunity, with consequences for the efficacy of emerging vaccines and antibody therapeutics. Herein we demonstrate that the immunodominant SARS-CoV-2 spike (S) receptor binding motif (RBM) is the most divergent region of S, and provide epidemiological, clinical, and molecular characterization of a prevalent RBM variant, N439K. We demonstrate that N439K S protein has enhanced binding affinity to the hACE2 receptor, and that N439K virus has similar clinical outcomes and in vitro replication fitness as compared to wild- type. We observed that the N439K mutation resulted in immune escape from a panel of neutralizing monoclonal antibodies, including one in clinical trials, as well as from polyclonal sera from a sizeable fraction of persons recovered from infection. Immune evasion mutations that maintain virulence and fitness such as N439K can emerge within SARS-CoV-2 S, highlighting the need for ongoing molecular surveillance to guide development and usage of vaccines and therapeutics.

Published
2020

49. N-terminal domain antigenic mapping reveals a site of vulnerability for SARS-CoV-2

Author

McCallum, Matthew, primary, De Marco, Anna, additional, Lempp, Florian A., additional, Tortorici, M. Alejandra, additional, Pinto, Dora, additional, Walls, Alexandra C., additional, Beltramello, Martina, additional, Chen, Alex, additional, Liu, Zhuoming, additional, Zatta, Fabrizia, additional, Zepeda, Samantha, additional, di Iulio, Julia, additional, Bowen, John E., additional, Montiel-Ruiz, Martin, additional, Zhou, Jiayi, additional, Rosen, Laura E., additional, Bianchi, Siro, additional, Guarino, Barbara, additional, Fregni, Chiara Silacci, additional, Abdelnabi, Rana, additional, Foo, Shi-Yan Caroline, additional, Rothlauf, Paul W., additional, Bloyet, Louis-Marie, additional, Benigni, Fabio, additional, Cameroni, Elisabetta, additional, Neyts, Johan, additional, Riva, Agostino, additional, Snell, Gyorgy, additional, Telenti, Amalio, additional, Whelan, Sean P.J., additional, Virgin, Herbert W., additional, Corti, Davide, additional, Pizzuto, Matteo Samuele, additional, and Veesler, David, additional

Published
2021
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