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Geophysical studies at Site H of Twin Cities Army Ammunition Plant have delineated specific areas of dumping and waste disposal. Anomalous areas noted in the geophysical data sets have been correlated with features visible in a chronological sequence of aerial photos. The photos aid in dating the anthropogenic changes and in interpreting the geophysical anomalies observed at Site H and across Sunfish Lake. Specifically, two burn cages and what has been interpreted as their surrounding debris have been delineated. The areal extent of another waste site has been defined in the southwest corner of Area H-1. Depth estimates to the top of the Area H-1 anomalies show that the anomalies lie below lake level, indicative of dumping directly into Sunfish Lake. Except for these areas along the northwestern shore, there is no evidence of waste disposal along the shoreline or within the present-day lake margins. Magnetic, electromagnetic, and ground-penetrating-radar data have pinpointed the locations of mounds, observable in aerial photos, around the first burn cage. The second burn cage and its surrounding area have also been clearly defined from aerial photos, with support from further geophysical data. Additional analysis of the data has yielded volumetric estimates of the amount of material that would need removal in the event of excavation of the anomalous areas. Magnetic and electromagnetic profiles were also run across Marsden Lake. On the basis of these data, it has been concluded that no large-scale dumping has occurred in or around Marsden Lake.

Exposure of ‘leaky’ adrenal medullary cells to GTP-γ-S inhibits Ca-dependent exocytosis in bovine cells, but stimulates exocytosis in chicken cells. The inhibitory action on bovine cells persists in the presence of TPA suggesting that in this tissue an inhibitory GTP-binding protein may modulate the action of protein kinase C on exocytosis.

Anextreme ultraviolet spectrometer experiment by University College London for observation of whole-Sun spectrum -line fluxes, and flown on Orbiting Solar Observatory-6, is described. Results for the four lines with absolute calibration, are show n for the first 6 months of flight, from August 1969 to February 1970. The mean fluxes for the 30.4, 53.7, 58.4 and 121.6 nm lines are 8.1 x 10 9 ± 35 % ph cm-2 s-1 , 3.2 x 10 8 + 40% ph cm -2 s-1 , 2 .5 x 10 9 + 40% ph cm -2 s-1 , and 3 x 10 11 ± 50% ph cm -2 s -1 respectively. Relations between these fluxes and the 10.7 cm radio flux and Zurich sunspot number are presented.

Enhancement by thrombin of Ca2+ -dependent 5HT secretion in the absence of added GTP decreases as the time between electropermeabilisation and addition of thrombin is increased. No decrease occurs if thrombin is added with GTP. Observation of apparent GTP-independent receptor/phospholipase C coupling may result from the presence of bound GTP in the preparation. Enhancement by GTP of Ca2+ -dependent 5HT secretion occurs with a significant lag indicating an agonist-independent effect. Cyclic 3′,5′-AMP inhibits enhancement by GTP of Ca2+ -dependent 5HT secretion while having no effect on enhancement induced by GTPγS. Hence cyclic AMP may impair receptor/phospholipase C coupling by enhancing Np GTPase activity.

Exposure of ‘leaky’ bovine adrenal medullary cells to the phorbol ester TPA causes a shift in the calcium-activation curve to lower calcium concentrations without altering the levels of secretion at the extremes of the activation curve. These results are consistent with a role for protein kinase C in exocytosis.

Addition of GTP markedly enhances the ability of thrombin to cause a leftward shift in the Ca 2+ dose/ response curve for 5-hydroxytryptamine secretion from permeabilised human platelets. Little effect is observed on addition of GTP in the absence of thrombin. Neither ADP nor adrenaline, in the presence or absence of GTP, causes such a shift, whereas 5-hydroxytryptamine does so to a small extent but only in the presence of GTP. The leftward shift in the Ca 2+ dose/response curve induced by 12- O -tetradecanoyl-phorbol-13-acetate or 1-oleyl-2-acetylglycerol is not enhanced by addition of GTP. The thrombin concentration required for half-maximal enhancement of the responce to Ca 2+ is markedly reduced by addition of GTP. The results support the postulate that the effects of excitatory agonists in this system correlate with their ability to activate phospholipase C and provide further evidence for a role for GTP in signal transduction between the receptor and phospholipase C.