6 results on '"D. G. Parry"'
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2. Proinsulin biosynthesis in broken-cell preparations of islets of Langerhans
- Author
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K. W. Taylor and D G Parry
- Subjects
Male ,endocrine system ,Sucrose ,medicine.medical_treatment ,Carbohydrates ,In Vitro Techniques ,Cycloheximide ,Biology ,Biochemistry ,Sepharose ,Islets of Langerhans ,Sonication ,chemistry.chemical_compound ,Biosynthesis ,Leucine ,medicine ,Animals ,Molecular Biology ,Proinsulin ,Cell-Free System ,Insulin ,Cell Biology ,Trypsin ,chemistry ,Electrophoresis, Polyacrylamide Gel ,Rabbits ,Research Article ,medicine.drug - Abstract
1. Rabbit islets of Langerhans were disrupted by ultrasonic methods and the sonicated preparations were used to study proinsulin biosynthesis. 2. When [3h]leucine is incubated in such preparations, incorporation takes place into proinsulin, as evidenced by characterization on polyacrylamide gels, and by the conversion of this labelled material into insulin, by using trypsin. 3. The labelled proinsulin may also be purified by antiinsulin antibody bound to Sepharose. 4. With the broken-cell preparation it was shown that incorporation of leucine is accelerated by increasing the glucose content of the medium from 2mM to 16mM. However, 16mM-galactose or -sucrose did not stimulate incorporation significantly from basal values. This effect of glucose was abolished by cycloheximide. 5. The significance of these findings in relation to the mechanism of glucose stimulation of proinsulin biosynthesis is discussed.
- Published
- 1978
- Full Text
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3. Seventh Annual Meeting of the European Association for the Study of Diabetes
- Author
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Bo Hellman, G. C. Palmieri, N. Mihalache, P. Siltanen, G. Bagnariol, W. K. Waldhäusl, M. Javicoli, U. Klör, F.A. Van Assche, M. Rathery, O. Melogli, D. Fedele, E. F. Pfeiffer, G. K. Rastogi, M. Bretán, R. R. de Mowbray, Poul Ebbe Nielsen, A. E. Lambert, Aa. Prange Hansen, Guido Tamburrano, J. C. Sodovez, B. Riveline, J. Canivet, Bartelt Km, S. Efendic, Franco Camanni, J. P. Bali, J. R. Claude, C. Rouiller, B. Schulz, M. Hinz, E. Eschwege, M. M. Mariani, M. Vigas, L. Kammerer, G. Enzi, M. Prud'homme, F. Massi-Benedetti, P. P. Foà, A. Ghidoni, I. M. Burr, L. Niklas, L. Nye, Sotirios Raptis, U. Brinck, B. Meyer, Hamish W. Sutherland, H. Laube, W. R. Drucker, B. Lesobre, H. J. Quabbe, M. A. Page, Åke Lernmark, Raimundo Goberna, M. Muggio, A. van't Laar, R. F. Murphy, Jens F. Rehfeld, Rolf Luft, C. E. Østerby, H. Karmann, K. E. Schröder, Ch. Thum, K. A. Munday, N. Mosora, Y. Kanazawa, D. P. Cameron, C. V. Tompkins, R. E. Levett, P. H. Sönksen, Domenico Andreani, R. D. M. Scott, P. J. Reeds, R. Fellin, A. Loubatières, M. J. Smith, J. Samsel, H. Iwatsuka, A. J. Valleron, S. Persson, K. Pyörälä, Victor Conard, J. M. Warnet, P. Polosa, R. Sparthe, A. Gordon, Y. Abdel Rahman, E. Fusco, Felix P. N. Schennetten, M. C. Srivastava, K. Johansen, D. Wübbens, F. Dauchy, Keith D. Buchanan, J. Marco, W. Teller, W. Poser, J. D. N. Nabarro, M. Rousselet, A. Hasselblatt, G. Rothenbuchner, H. Ørskov, G. M. Molinatti, J. Schönborn, A. Vitelli, A. M. McCarroll, Inge Bert Täljedal, M. Amherdt, R. Knussman, F. A. Gries, L. Orci, Beyer J, F. Jallet, J. Schlichtkrull, Z. Skrabalo, J. Vincze, W. Korp, Schöffling K, Ev. Kriedstein, E. B. Möller, J. Landon, S. Frezzato, H. Wingstrand, F. Massara, G. E. Rosselin, G. J. A. I. Snodgrass, H. L. Fehm, D. Michaelis, S. Le Guilcher, K. Seyer-Hansen, C. Kruger, D. M. Kipnis, J. Mirouze, María L. Villanueva, O. Oelz, P. J. Lefèbvre, V. Duma, Per Westermark, E. Giangrandi, M. R. Turner, H. Bibergeil, G. R. Brisson, J. Birk, T. Mincu, I. M. Baroja, M. R. P. Hall, G. Wick, Patricia Metzger, Werner Oppermann, E. Jutzi, Guido Pozza, P. Jacquet, A. Kopf, J Ostman, N. Conte, R. Fuchs, Theodore Ehrenreich, I. Mincu, Barbara Rudas, A. S. Luyckx, A. E. Renold, A. Schirmann, U. Klemens, R. E. Haist, V. Nuteanu, L. Papoz, R. Spaethe, G. Tohobroutsky, W. Hildmann, V. Gligore, B. Morell, G. Tchobroutsky, Willy Malaisse, J. Sterne, G. Löffler, N. S. Track, E. B. Marliss, EskoA. Nikkilä, C. R. C. Heard, Tr. Baciu, F. Fallucca, E. Krug, J. Trap-Jensen, Isabel Valverde, Rafael A. Camerini-Davalos, S. Klahr, L. Stimmler, C. Rosak, M. Motocou, K. W. Taylor, H. Otto, O. Wieland, K. Lundbak, Th. Koschinsky, R. Assan, E. Cerasi, M. Toeller, S. Triggs, W. Stauffacher, Haupt E, R. J. Dash, C. Scandellari, E. R. Froesch, F. J. Woodroffe, L. Balant, M. Verry, M. Lunetta, A. Tiengo, D. G. Parry, L. Weiss, M. Kikuchi, Uwe Panten, G. C. Viberti, B. Blondel, J. D. Teale, J. C. Dunbar, N. S. Bricker, C. E. Ruth, M. K. Sinha, W. Braun, M.R. Taskinen, D. H. Williamson, U. Loos, O. Steingaszner, Giovanni Federspil, L. Herberg, S. Georgescu, R. Fussgänger, J. Hewitt, A. L. Bergström, S. Levin Nielsen, H. Schatz, E. Lehtovirta, Jurgen Steinke, I. Lozano, F. Sodovez-Goffaux, D. R. Langslow, E. Speich, I. Neumann, G. Menzinger, A. Tinant, S. Munkgaard Rasmussen, Janove Sehlin, C. Dumitrescu, N. Katsilambros, A. Tognetti, Ligia Simionesco, C. Oliver, C. E. Mogensen, A. E. Pappalettera, P. Vague, K. Sträub, L. Motta, J. Vague, A. Orsetti, Mme A. Serre, B. M. Freeman, A. Trisotto, R. Korec, M. Diaz-Fierros, F. Stadil, S. Campeanu, J. Sabin, H. P. T. Ammon, P. Mialhe, F. Legros, C. Rogister, R. Schröder, V. Maier, Risto Pelkonen, F. Scaroina, M. Parvulescu, and K. E. Schenk
- Subjects
medicine.medical_specialty ,business.industry ,Endocrinology, Diabetes and Metabolism ,Association (object-oriented programming) ,Family medicine ,Ophthalmology ,Diabetes mellitus ,Internal Medicine ,medicine ,Human physiology ,business ,medicine.disease - Published
- 1972
- Full Text
- View/download PDF
4. The effects of sugars on incorporation of [3H)leucine into insulins
- Author
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K. W. Taylor and D. G. Parry
- Subjects
Electrophoresis ,Chromatography, Paper ,General Mathematics ,medicine.medical_treatment ,Mannose ,Tritium ,chemistry.chemical_compound ,Leucine ,medicine ,Animals ,Insulin ,Pancreas ,Applied Mathematics ,Galactose ,Rats ,Paper chromatography ,Glucose ,chemistry ,Biochemistry ,Cattle ,3h leucine ,Research Article - Published
- 1966
5. BIOSYNTHETIC LABELLING OF MAMMALIAN INSULINS IN VITRO
- Author
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K. W. Taylor, D. G. Parry, and G. Howard Smith
- Subjects
medicine.medical_treatment ,Insulins ,In Vitro Techniques ,Tritium ,Leucine ,Labelling ,medicine ,Animals ,Insulin ,Isoleucine ,Radiometry ,Pancreas ,Mammals ,Chromatography ,Multidisciplinary ,Chemistry ,Research ,Fishes ,In vitro ,medicine.anatomical_structure ,Glucose ,Biochemistry ,Cattle - Published
- 1964
6. Secretion of newly synthesized insulin in vitro
- Author
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S. L. Howell, K. W. Taylor, and D. G. Parry
- Subjects
medicine.medical_specialty ,Multidisciplinary ,Chemistry ,Insulin ,medicine.medical_treatment ,Insulin Antibodies ,Cell ,Stimulation ,Peptide hormone ,In Vitro Techniques ,In vitro ,Islets of Langerhans ,Endocrinology ,medicine.anatomical_structure ,Glucose ,Internal medicine ,medicine ,Animals ,Secretion ,Rabbits ,gamma-Globulins ,Pancreas ,Secretory Rate ,Endocrine gland - Abstract
UP to the present, there has been little information available concerning the rate at which newly synthesized protein hormones may be secreted, following the stimulation of endocrine glands. In recent work on the synthesis of insulin within pancreas slices, in vitro, it became of interest to determine whether or not newly synthesized insulin may be released directly into the incubation medium, or alternatively whether or not release of insulin might be delayed following its accumulation within β-granules. It has already been suggested by Lacy1 that release of insulin from β-cells in response to glucose involves the movement of these granules to the cell surface, where they are released into blood vessels.
- Published
- 1965
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