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Made available in DSpace on 2020-12-10T18:00:40Z (GMT). No. of bitstreams: 0 Previous issue date: 2000-04-01 The effects of treatment with anaerobic cecal microflora (ACM) and/or lactose and/or acetic acid on systemic and digestive tract of broiler chicks infection with Salmonella Typhimurium and S. Enteritidis were studied. ACM was used without previous bacterial identification. Treatment with ACM contributed to the resistance of broiler chicks to infection with Salmonella spp. The infections were mon persistent in the cecum, rectum and crops in decreasing order of intensity. The infections were also self-limiting since treated and control lots presented similar infection rates at the end of the experiments. Alone or in combination with lactose, ACM reduced the colonization of the digestive tract of broiler chicks by S. Typhimurium and S. Enteritidis. The effect of the combination of ACM with lactose or acetic acid was not potentiated in terms of reduction of fecal excretion of Salmonella spp. Treatment with ACM reduced the amount of S. Typhimurium and S. Enteritidis in the feces. Alone or in combination with lactose, ACM reduced the cecal pH in treated birds. S. Enteritidis was much more invasive than S. Typhimurium and the use of ACM alone was more effective on the reduction of systemic infection. An explanation for the process of prevention of intestinal colonization with Salmonella spp. probably resides in the interrelationship of physiological, microbiological and immunological phenomena, as well as the variation in cecal pH. UNESP, Fac Med Vet & Zootecnia, Dept Clin Vet, BR-18618000 Botucatu, SP, Brazil UNESP, Fac Med Vet & Zootecnia, Dept Melhoramento & Nutr Anim, BR-18618000 Botucatu, SP, Brazil Univ Estadual Campinas, Fac Engn Alimentos, Dept Tecnol Alimentos, Campinas, SP, Brazil UNESP, Fac Med Vet & Zootecnia, Dept Clin Vet, BR-18618000 Botucatu, SP, Brazil UNESP, Fac Med Vet & Zootecnia, Dept Melhoramento & Nutr Anim, BR-18618000 Botucatu, SP, Brazil

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2014-02-26T17:18:29Z No. of bitstreams: 1 WOS000076517000005.pdf: 5211128 bytes, checksum: b9d54f315239f19ca0f23fe10f0cdfb7 (MD5) Made available in DSpace on 2014-02-26T17:18:29Z (GMT). No. of bitstreams: 1 WOS000076517000005.pdf: 5211128 bytes, checksum: b9d54f315239f19ca0f23fe10f0cdfb7 (MD5) Previous issue date: 1998-06-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:28:27Z No. of bitstreams: 1 WOS000076517000005.pdf: 5211128 bytes, checksum: b9d54f315239f19ca0f23fe10f0cdfb7 (MD5) Made available in DSpace on 2014-05-20T15:28:27Z (GMT). No. of bitstreams: 1 WOS000076517000005.pdf: 5211128 bytes, checksum: b9d54f315239f19ca0f23fe10f0cdfb7 (MD5) Previous issue date: 1998-06-01 Blood samples from lactating, weaned and adult Holstein-Fresiean and Nelore cattle and Murrah buffaloes were tested through the study of hemogram and serum constituents. Red cell and leukocyte counts, and blood pH, fibrinogen, glucose, calcium, and phosphorus levels were similar in cattle and buffalo. Total protein, urea, creatinine, hemoglobin, magnesium, iron and aspartato aminotransferase and alanine aminotransferase activities were higher in buffalo than cattle. Leukocyte counts were higher for weaned Holstein cattle than for Nelore cattle but similar to buffalo and Nelore cattle had the lowest neutrophil counts. Bilirrubin levels were lower for buffalo than for cattle. Phosphatase alkalyne activities were lower for weaned buffalo than for other animals. Gammaglutamyltransferase activities were the highest for lactating and weaned buffalo. UNESP, Fac Ciências Agrarias & Vet, BR-14870000 Jaboticabal, SP, Brazil UNESP, FCAV, Jaboticabal, SP, Brazil UNESP, FMVZ, Botucatu, SP, Brazil UNESP, Fac Ciências Agrarias & Vet, BR-14870000 Jaboticabal, SP, Brazil UNESP, FCAV, Jaboticabal, SP, Brazil UNESP, FMVZ, Botucatu, SP, Brazil

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2014-02-26T17:18:30Z No. of bitstreams: 1 WOS000076517000006.pdf: 4642944 bytes, checksum: 372e1fea64f4e20739d7b3d48431d694 (MD5) Made available in DSpace on 2014-02-26T17:18:30Z (GMT). No. of bitstreams: 1 WOS000076517000006.pdf: 4642944 bytes, checksum: 372e1fea64f4e20739d7b3d48431d694 (MD5) Previous issue date: 1998-06-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:25:46Z No. of bitstreams: 1 WOS000076517000006.pdf: 4642944 bytes, checksum: 372e1fea64f4e20739d7b3d48431d694 (MD5) Made available in DSpace on 2014-05-20T15:25:46Z (GMT). No. of bitstreams: 1 WOS000076517000006.pdf: 4642944 bytes, checksum: 372e1fea64f4e20739d7b3d48431d694 (MD5) Previous issue date: 1998-06-01 Blood constituents of Nelore, Hosltein and buffalo cows were determined during pregnancy, at the time of calving and at post-partum. It was verified that. 1. The pregnancy and post-partum periods had no influence in the erythrocyte values, but at calving the red blood cell counts and hemoglobin levels of Holstein and buffalo cows were lower than for Nelore cattle; 2. leukocyte counts were similar among groups; 3. total protein levels of Nelore cows were lower than Holstein and buffalo. The albumin levels were rite lowest at rite time of calving compared to late pregnancy; 4. glicose levels were lower ill buffalo cows during pregnancy as compared with Holstein cows. The glicemia of Nelore cows was lower as compared to Holstein cow's irt late pregnancy; 5. urea and creatinine levels were higher in buffalo cows than cattle. The urea and creatinine levels were greater in buffalo cows with maximum values at the post-partum and at the parturition, respectively; 6. bilirrubin levels were higher in bovine than buffalo cows; 7. aspartate aminotransferase activity was greater in buffalo cows and increased at the time of calving; 8. alkaline phosphatase activity was increased during pregnancy and decreased after the time of calving; 9. gammnglutamyltransferase activity,vas the highest for buffalo cows after calving; IO. calcium levels were the highest for Holstein cows at the post-partum and the phosphorus levels were higher in buffalo cows, which had the highest magnesium levels at the parturation. UNESP, Fac Cienicias Agrarias & Vet, BR-14870000 Jaboticabal, SP, Brazil UNESP, FMVZ, Botucatu, SP, Brazil UNESP, Fac Cienicias Agrarias & Vet, BR-14870000 Jaboticabal, SP, Brazil UNESP, FMVZ, Botucatu, SP, Brazil

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2014-02-26T17:18:27Z No. of bitstreams: 1 WOS000076517000004.pdf: 4833228 bytes, checksum: 5ecdfd16dacb43e01488fae67ea8156c (MD5) Made available in DSpace on 2014-02-26T17:18:27Z (GMT). No. of bitstreams: 1 WOS000076517000004.pdf: 4833228 bytes, checksum: 5ecdfd16dacb43e01488fae67ea8156c (MD5) Previous issue date: 1998-06-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:25:45Z No. of bitstreams: 1 WOS000076517000004.pdf: 4833228 bytes, checksum: 5ecdfd16dacb43e01488fae67ea8156c (MD5) Made available in DSpace on 2014-05-20T15:25:45Z (GMT). No. of bitstreams: 1 WOS000076517000004.pdf: 4833228 bytes, checksum: 5ecdfd16dacb43e01488fae67ea8156c (MD5) Previous issue date: 1998-06-01 Newborn Holstein-Friesian and Nelore cattle and Murrah buffalo blood samples were collected for hemograma and serum constituents testing. Results indicated that: 1. buffalo red blood cell (RBC) counts were lower than RBC counts for cattle during the first 30 days after birth; 2. buffalo leukocytes, neutrophil and lymphocyte counts increased in older animals; 3. The highest fibrinogen levels occurred in older calves; 4. buffalo glucose levels were lower than Nelore cattle and decreased in the older calves; 5. buffalo iron, urea, and aspartate aminotransferase levels were higher than in cattle with the highest concentration at the time of birth; 6. cattle bilirubin and alanine aminotranferase levels were higher than buffalo with the highest value at the time of birth; 7. alkaline phosphatase and gammaglutamyltransferase activities were lower in the older calves; 8. buffalo magnesium levels were higher than cattle with lower concentrations at the time of birth.. UNESP, Fac Ciências Agrarias & Vet, BR-14870000 Jaboticabal, SP, Brazil UNESP, FCAVJ, Jaboticabal, SP, Brazil UNESP, FMVZ, Botucatu, SP, Brazil UNESP, Fac Ciências Agrarias & Vet, BR-14870000 Jaboticabal, SP, Brazil UNESP, FCAVJ, Jaboticabal, SP, Brazil UNESP, FMVZ, Botucatu, SP, Brazil

Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:18:16Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:38:54Z : No. of bitstreams: 1 2-s2.0-0030863903.pdf: 62302 bytes, checksum: cb3dcb88acf8896f54dd9243b4b15a0e (MD5) Made available in DSpace on 2014-05-27T11:18:16Z (GMT). No. of bitstreams: 0 Previous issue date: 1997-10-15 Background and Objectives - Pulmonary aspiration of gastric content during induction of anesthesia for emergency surgical procedures is a serious complication; fast endotracheal intubation under these circumstances is of vital importance to secure the airways. Despite its numerous side effects, succinylcholine is used for this purpose. Rocuronium is the most recently introduced aminoesteroid neuromuscular blocking drug with short onset. The objective of this study was to compare the onset time and intubating conditions of rocuronium and succinylcholine. Methods - After informed consent, forty-five patients were randomly allocated into three groups of 15: Group I (GI) = succinylcholine 1 mg.kg-1; Group II (GII) = rocuronium 0.6 mg.kg-1; Group III (GIII) = rocuronium 0.9 mg.kg-1. Every patient was premedicated with midazolam 15 mg per os and induction of anesthesia was made with fentanyl 10 μg.kg-1 and etomidate 0.3 mg.kg-1. The neuromuscular block was monitored with the TOP-Guard neuromuscular transmission monitor. The TOP-Guard neuromuscular monitor uses an accelerometer to measure the response to nerve stimulation. The stimulating electrodes were placed close to the course of the ulnar nerve at the wrist. The onset time was considered as the time between the end of neuromuscular drug injection and the twitch height (T1) decrease to 10%. Heart rate and arterial blood pressure were registered at 6 moments before and after induction of anesthesia. Results - The onset time results were: Group I, 71 s; Group II, 120 s and Group III, 70 s or GI = GIII < GII (F = 8.862; p < 0.01). There were 43 patients exhibiting excellent intubating conditions and 2 with good intubating conditions. Heart rate and arterial blood pressure showed alterations due to induction of anesthesia and intubation. Conclusions - Rocuronium 0.9 mg.kg-1 can be used in rapid sequence induction because it has a short onset time which is similar to that of succnylcholine. It is likely that rocuronium would be a good indication in patients with high intracranial pressure, burns and neuromuscular diseases. UNESP Depto. de Anestesiologia, Rubiao Junior, 18618-970 Botucatu-SP UNESP Depto. de Anestesiologia, Rubiao Junior, 18618-970 Botucatu-SP

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2014-02-26T17:08:46Z No. of bitstreams: 1 WOSA1997XU89300002.pdf: 5140955 bytes, checksum: e3de0a1ec4398f43a0f098820ed9046b (MD5) Made available in DSpace on 2014-02-26T17:08:46Z (GMT). No. of bitstreams: 1 WOSA1997XU89300002.pdf: 5140955 bytes, checksum: e3de0a1ec4398f43a0f098820ed9046b (MD5) Previous issue date: 1997-06-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:22:10Z No. of bitstreams: 1 WOSA1997XU89300002.pdf: 5140955 bytes, checksum: e3de0a1ec4398f43a0f098820ed9046b (MD5) Made available in DSpace on 2014-05-20T15:22:10Z (GMT). No. of bitstreams: 1 WOSA1997XU89300002.pdf: 5140955 bytes, checksum: e3de0a1ec4398f43a0f098820ed9046b (MD5) Previous issue date: 1997-06-01 Two groups of Holstein-Friesian and Nelore calves, five animals each, about nine months old, received, by oral route, 1,000 infective larvae (L-3) per kg of body weight of Haemonchus placei. Blood samples were collected by venipuncture, at weekly intervals, from one week before, to eight weeks after infection. Hematological studies comprised the hematocrit, differential leukocyte counts, hemoglobin, fibrinogen and plasma protein determinations. Parasitological examinations covered weekly fecal egg counts (EPG) and worm burden counts at necropsy. Samples of the abomasal mucosa were submitted to gross examination and histopathological studies. Both groups had increasing EPG after the fifth week, with Holstein calves showing higher counts than the Nelore. Holstein calves had anemia and hipoproteinemia from the third week post-infection to the end of the experiment, whereas Nelore calves showed no significant differences in those, parameters. Holstein calves had significantly larger worm counts than the Nelore. The gross and histopathological lesions in the abomasum at necropsy were very similar, although macroscopically they look more apparent in the Holstein group. These results showed that Holstein calves are more susceptible to the infection and pathogenic effects of H. placei than Nelore calves. UNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,FMVZ,DEPT CLIN VET,CAIXA POSTAL 560,BR-18618000 BOTUCATU,SP,BRAZIL UNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,FMVZ,DEPT CLIN VET,CAIXA POSTAL 560,BR-18618000 BOTUCATU,SP,BRAZIL

Made available in DSpace on 2022-04-28T19:54:17Z (GMT). No. of bitstreams: 0 Previous issue date: 1995-01-01 Thirty-two patients bitten by venomous snakes sixteen by Bothrops spp. and sixteen by Crotalus durissus terrificus were studied. The group comprised thirty males and two females, aged eight to sixty-three years (mean 33 +/- 15). Bromsulphalein tests were increased in the majority of patients bitten by Crotalus durissus terrificus. The correlation coefficient of Spearman was positive between bromsulphalein tests and alanine aminotransferase levels, and between alanine aminotransferase and aspartate aminotransferase levels only in the Crotalus group. The only patient who died was bitten by Crotalus durissus terrificus and showed hydropic degeneration and mitochondrial injury in the liver. It was concluded that the hepatic damage might have been caused by at least two possible mechanisms: venom effect on liver mitochondria and cytokine effects on hepatocyte, specially interleukin-6. Departamento de Doenças Tropicais e Diagnóstico por Imagem Faculdade de Medicina de Botucatu - UNESP Departamento de Doenças Tropicais e Diagnóstico por Imagem Faculdade de Medicina de Botucatu - UNESP

Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:17:55Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:28:16Z : No. of bitstreams: 1 2-s2.0-0028355925.pdf: 55525 bytes, checksum: 606be18cdf3cfe82315ba7e40cf9e1eb (MD5) Made available in DSpace on 2014-05-27T11:17:55Z (GMT). No. of bitstreams: 0 Previous issue date: 1994-01-01 Little research has been done with propofol in relation to renal function. The aim of this study was to evaluate the effects of the continuous infusion of propofol on renal function in dogs. Sixteen dogs, previously anesthetized with pentobarbital sodium (30 mg.kg-1) for surgical preparation, catheterism and monitoring, were studied. The dogs were mechanically ventilated with air and received alcuronium (0.2 mg.kg-1 in bolus and 0.06 mg.kg-1 - maintenance). The following parameters were studied: heart rate (HR), mean arterial pressure (MAP), central venous pressure (CVP), aortic blood flow (A(o)BF - by electromagnetic flowmeter installed in the ascending aortic), aortic vascular resistance index (A(o)VRI), renal plasma flow (ERPF - by para-aminohipurate clearance), glomerular filtration rate (GFR - by creatinine clearance), effective renal blood flow (ERBF = ERPF/1 - hematocrit), urinary volume (UV), renal vascular resistance (RVR = MAP.80/ERBF.10-3), urinary sodium excretion (UE(Na)), fractionated sodium excretion (FE(Na)), osmolar clearance (C(osm)) and free water clearance (C(H2O)). These parameters were studied at 15 (M1), 30 (M2), 45 (M3) and 60 (M4) min after beginning pentobarbital sodium infusion (5 mg.kg-1.h-1). The dogs were allocated into two groups of eight animals each: G1 (control-pentobarbital sodium) and G2 (propofol). In G1, pentobarbital was given at the four times studied. G2 dogs received the same treatment as G1 dogs at M1 and M2; infusion of pentobarbital was substituted by propofol (3 mg.kg-1 bolus, followed by 12 mg.kg-1.h-1 continuous infusion) at M3 and M4. Profile Analysis was used to analyze the results statistically. In G1 (pentobarbital), there was a significant increase in RVR (M1 < M4) and a decrease in ERPF and ERBF (M1 > M4). In G2 (propofol) there was only a significant increase in A(o)BF (M1 < M2 = M3). In comparison among groups, these was a significant alteration of FE(Na) at M3 (pentobarbital > propofol). It was observed that the continuous infusion of propofol in dogs, at the given doses, did not alter the basic variables of renal function and hemodynamics studied. We concluded that propofol can be one of the drugs of choice to provide base anesthesia in studies of renal function in dogs. Departamento de Anestesiologia Faculdado de Medicina UNESP, 18618-000 Rubiao Junior, Botucatu Departamento de Anestesiologia Faculdado de Medicina UNESP, 18618-000 Rubiao Junior, Botucatu

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2014-02-26T17:04:50Z No. of bitstreams: 0 Made available in DSpace on 2014-02-26T17:04:50Z (GMT). No. of bitstreams: 0 Previous issue date: 1992-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:22:30Z No. of bitstreams: 0 Made available in DSpace on 2014-05-20T15:22:30Z (GMT). No. of bitstreams: 0 Previous issue date: 1992-01-01 Calomys callosus a wild rodent, previously described as harboring Trypanosoma cruzi, has a low susceptibility to infection by this protozoan.Experiments were designed to evaluate the contribution of the immune response to the resistance to T. cruzi infection exhibited by C. callosus. Animals were submitted to injections of high (200 mg/kg body weight) and low (20 mg/kg body weight) doses of cyclophosphamide on days -1 or -1 and +5, and inoculated with 4 x 10(3) T. cruzi on day O. Parasitemia, mortality and antibody response as measured by direct agglutination of trypomastigotes were observed. Two hundred mg doses of cyclophosphamide resulted in higher parasitemia and mortality as well as in suppression of the antibody response. A single dose of 20 mg enhanced antibody levels on the 20th day after infection, while an additional dose did not further increase antibody production. Parasitemia levels were not depressed, but rather increased in both these groups as compared to untreated controls. Passive transfer of hyperimmune C. callosus anti-T. cruzi serum to cyclophosphamide immunosuppressed animals resulted in lower parasitemia and mortality rates. These results indicate that the immune response plays an important role in the resistance of C. callosus to T. cruzi. INST MED TROP SAO PAULO,SAO PAULO,BRAZIL UNIV ESTADUAL PAULISTA,INST BASICO BIOL MED & AGR,DEPT STAT,BOTUCATU,SP,BRAZIL UNIV SAO PAULO,INST CIENCIAS BIOMED,DEPT PARASITOL,SAO PAULO,BRAZIL UNIV ESTADUAL PAULISTA,INST BASICO BIOL MED & AGR,DEPT STAT,BOTUCATU,SP,BRAZIL

No presente estudo de grupos sanguineos de bovinos da regiao de Botucatu, SP, foram utilizados 148 bovinos puros de origem, mesticos, adultos e de ambos os sexos, das racas Pardo-Suico, Girolanda, Pitangueiras, Gir e Nelore. De cada bovino foram coletados 10 ml de sangue venoso em frascos com anticoagulante. Os grupos sanguineos foram identificados atraves de testes hemoliticos, conforme a tecnica de Stormont, utilizando-se 42 reagentes de tipagem sanguinea, especificos para bovinos. A maioria dos fatores sanguineos presentes com frequencia alta e significativa (P

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2014-02-26T17:05:08Z No. of bitstreams: 0 Made available in DSpace on 2014-02-26T17:05:08Z (GMT). No. of bitstreams: 0 Previous issue date: 1992-12-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:21:00Z No. of bitstreams: 0 Made available in DSpace on 2014-05-20T15:21:00Z (GMT). No. of bitstreams: 0 Previous issue date: 1992-12-01 A área das fibras musculares esqueléticas, o peso corporal, da carcaça, de um músculo, o volume gástrico, o comprimento do ceco e dos intestinos foram avaliados em coelhos Norfolk, do nascimento à 26a semana. Para o estudo da área dos tipos de fibras musculares, segmentos do músculo flexor carpo-radial foram congelados a -70oC. Cortes obtidos em criostato com 8 m de espessura foram corados pela Hematoxilina e Eosina e outros, incubados para as enzimas: SDH, NADH-TR e m-ATPase, após pré-incubação em meio ácido e alcalino. Os dados foram submetidos a testes estatísticos de significância. As variáveis estudadas revelaram acentuada correlação entre si. A razão peso da carcaça/peso corporal atingiu 55,1% na 10a semana. Este valor revelou-se estável até a 22a semana, diminuindo nas idades subseqüentes. No periodo estudado, a área das fibras SO, FOG e FG aumentou 9,1 vezes, 12,4 vezes e 22,2 vezes respectivamente. Na 10a semana, as fibras aeróbicas SO e FOG tenderam a estabilizar seu crescimento, enquanto que as fibras anaeróbicas FG revelaram ativo crescimento. Com a estabilização do crescimento das fibras SO e FOG, o aumento da massa muscular apósa 10a semana parece ocorrer principalmente pela hipertrofia das fibras anaeróbicas FG. Skeletal muscle fibers areas, the weight of body, carcass and muscle, the gastric content and the length of intestines and caecum of Norfolk rabbit from neonate to 26th week were measured. Types of fibers and areas were studied in -70-degrees-C frozen blocks of flexor carpo radialis muscle. Some sections were stained with Haematoxylin and Eosin and others were submitted to SDH, NADH-TR and m-ATPase, followed by acid and alkali preincubations. All parameters revealed a high degree of correlation. Carcass weight/body weight ratio reached 55,1% at 10th week. From neonate to 26th week, the areas of SO, FOG and FG muscle fibers increased of x9.1, x12.4 and x22.2. While SO and FOG fibers tend to stabilize their growth at 10th week, FG fibers were active at this age. Due to early stabilization of SO and FOG fibers growth, the additional increase in muscle tissue in attributed mainly to hypertrophy of FG fibers. UNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,IB,DEPT MORFOL,BR-18600 BOTUCATU,SP,BRAZIL UNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,IB,DEPT MORFOL,BR-18600 BOTUCATU,SP,BRAZIL

Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:17:27Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:28:09Z : No. of bitstreams: 1 2-s2.0-0026833448.pdf: 166240 bytes, checksum: 8de5be06de4c886e9ea0c5cdb1dd4efe (MD5) Made available in DSpace on 2014-05-27T11:17:27Z (GMT). No. of bitstreams: 0 Previous issue date: 1992-01-01 Disciplina de Nefrologia Depto. Clinica Medical UNESP, 18610 Botucat Disciplina de Nefrologia Depto. Clinica Medical UNESP, 18610 Botucat

Made available in DSpace on 2022-04-29T08:42:21Z (GMT). No. of bitstreams: 0 Previous issue date: 1989-12-01 The present study evaluates platelet activation following application of an Esmarch bandage and a tourniquet, procedures commonly employed to provide a bloodless operative field during limb surgery. Platelet aggregation was increased in blood samples taken from rabbits 60 min after an Esmarch bandage was applied to one thigh and immediately released. When this treatment was combined with the application of a tourniquet for 60 min, a procedure which alone did not affect platelet aggregation, results were similar to those obtained following the Esmarch bandage alone. These data suggest that tissue compression produced by application of an Esmarch bandage, but not the ischemia derived from the tourniquet, produced platelet aggregation. Departamento de Cirurgia e Ortopedia, Hospital das Clínicas, Faculdade de Medicina de Botucatu, Universidade Estadual Paulista, Brasil.

Made available in DSpace on 2022-04-29T08:42:14Z (GMT). No. of bitstreams: 0 Previous issue date: 1982-12-01 Measurements of cross sectional area on individual fibres of M. gracilis (pars caudalis) of Wistar rats of both sexes of different ages were done. Growth equations pattern and growth rates equations pattern of each muscle fibre type were also determined. In rats 12 days old, muscle fibres present a somewhat uniform SDH activity. By 23 days and thereafter, SDH technique revealed a mixed population of red, intermediate and white fibres. There was a close relationship between cross sectional area and age, where each fibre type presents a distinct exponential growth curve pattern. The highest growth rates were observed at 23 and 45 days. After this age, a marked fall in growth rates occurs, except for white fibres, where this decrease was less intense. Red and intermediate muscle fibers tend to stabilize their growth earlier than white fibres. Dept. Morphol., State Univ. 'Julio de Mesquita Filho', Botucatu, Sao Paulo

Made available in DSpace on 2022-04-29T08:42:20Z (GMT). No. of bitstreams: 0 Previous issue date: 1989-12-01 Coronary sinus blood oxygen tension (CSpO2) and myocardial oxygen tension (MpO2) were measured simultaneously during cardiac ischemia and reperfusion. Oxygen tension was measured using a polarographic method. Myocardial ischemia caused a reduction (5 +/- 1.5%; P less than 0.05) in CSpO2 and a decrease (56.5 +/- 10.1%; P less than 0.001) in MpO2. Reperfusion induced a rapid but transient increase (35.9 +/- 4.3%; P less than 0.001) in CSpO2 above the basal state while MpO2 returned gradually to the basal state. These results indicate that CSpO2 is of little value for the detection of changes in myocardial oxygen metabolism during the initial phase (seconds) of cardiac reperfusion. Departamento de Clínica Médica, Faculdade de Medicina de Botucatu, Brasil.

Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-26T22:09:26Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:27:59Z : No. of bitstreams: 1 2-s2.0-0021163981.pdf: 1755150 bytes, checksum: 8244c1234bfbba06b25763a87bb75eac (MD5) Made available in DSpace on 2014-05-26T22:09:26Z (GMT). No. of bitstreams: 0 Previous issue date: 1984-01-01 To evaluate the effect of 5-fluorouracil (F) and methotrexate-5-fluorouracil association (MTX-F) on nephrotoxic nephritis, seven groups of 10 rats were inoculated with anti-rat glomerular basement membrane serum (AGBMS); five groups were treated with different doses of F, beginning on the 2nd or the 6th day, one group with MTX-F beginning on the 2nd day and one group (control) with distilled water. Twenty-four hour proteinuria was determined weekly until the 71st day. The kidneys were examined histologically and by immunofluorescence. The group treated with F (1.3 mg/100 g body weight) developed a severe glomerulonephritis similar to the control group; (b) the groups treated with F (2.0 mg/100 g body weight) or with MTX-F showed progressively lower proteinuria, less severe histological changes and less intense fluorescence due to autologous antibodies. The best results were observed in the MTX-F group and in the F group treated from the 6th day. These groups presented at the 71st day proteinuria of 84 and 91 mg as compared to 312 mg in the control group, and minimal histological lesions as compared to glomerulosclerosis and tubular atrophy in the control group. We concluded that either F or MTX-F produced significant improvement of nephrotoxic nephritis due to inhibition of autologous antibody production.

Made available in DSpace on 2022-04-29T08:42:16Z (GMT). No. of bitstreams: 0 Previous issue date: 1985-01-01 Department of Surgery and Orthopaedics, Faculty of Medicine of Botucatu, University Estadual Paulista, Sao Paulo

Made available in DSpace on 2022-04-28T18:53:30Z (GMT). No. of bitstreams: 0 Previous issue date: 1976-01-01 Dept. Matemat. Estatistica, Fac. Ci. Med. Biol., Botucatu

Made available in DSpace on 2022-04-29T08:42:18Z (GMT). No. of bitstreams: 0 Previous issue date: 1988-01-01 Departamento de Cirurgia e Ortopedia, Faculdade de Medicina de Botucatu, UNESP, 18610 Botucatu, SP

Made available in DSpace on 2022-04-28T18:53:57Z (GMT). No. of bitstreams: 0 Previous issue date: 1989-12-01 An experimental research was performed in dogs to evaluate the changes in gracilis muscle fibers when this muscle is submitted to denervation sectioning its motor nerve. Two experimental groups of six dogs each were used. Animals in Group A were sacrificed 30 ays after denervation of the gracilis muscle, ans animals in Group B were sacrificed 60 days after denervation. The normal gracilis muscle of the opposite leg was used as control. The degree of denervation was evaluated by two parameters: 1) the area of the cross section of the muscular fibers was measured by a planimeter; 2) the number of complete fibers seen in a specific microscopic field. Animals in both groups had atrophy of the muscular fibers, however atrophy was more intense in Group B (60 days) than in Group A (30 days). Conclusion was that the wieght of the denervated muscle is not an accurate method to study the evolution of the degenerating proces although it expresses the muscular atrophy resulting from the denervation. The number of muscular fibers in the microscopic field and the areas of the cross section of the muscular fibers obtained by planimetry are accurate parameters to detect the progress of muscular changes resulting from denervation. considering that it is easier to count the number of muscular fibers in the microscopic field, the authors propose the use of this preferential method in the study of the evolution of muscular changes due to muscle denervation. School of Medicine of Botucatu of the University of the State of Sao Paulo, Sao Paulo

Made available in DSpace on 2022-04-28T19:53:50Z (GMT). No. of bitstreams: 0 Previous issue date: 1991-01-01 1. The protection offered by intermittent perfusion of cardioplegic solution through the coronary sinus was investigated in isovolumic blood-perfused dog heart preparations submitted to 60 min of ischemia and 45 min of reperfusion. 2. The preparations were divided into three treatment groups: a) coronary sinus, consisting of preparations (N = 10) perfused through the coronary sinus under 40 cm water pressure; b) aortic, consisting of preparations (N = 10) perfused through the aortic stump under 100 mmHg pressure; c) control, consisting of hearts (N = 9) that were not perfused with cardioplegic solution. 3. Properties of contractile capacity and relaxation were markedly impaired in the control group but were preserved to a comparable extent in the groups perfused with cardioplegic solution through the aorta and coronary sinus. Developed pressure decreased in the control group (before ischemia: 70 +/- 5.5 mmHg; after reperfusion: 35 +/- 12 mmHg; P less than 0.05) and didn't vary in the aortic group (from 69 +/- 4 mmHg to 65 +/- 13 mmHg; P greater than 0.05) and coronary sinus group (from 69 +/- 4.6 mmHg to 60 +/- 10 mmHg; P greater than 0.05). Myocardial relaxation was evaluated by the +/- dp/dt ratio. In the control group there was impairment of myocardial relaxation as indicated by an increase of this index after reperfusion (from 1.05 +/- 0.05 to 1.46 +/- 0.23; P less than 0.05), whereas in the aortic (from 1.10 +/- 0.13 to 1.15 +/- 0.20; P greater than 0.05) and the coronary sinus (from 1.03 +/- 0.14 to 1.08 +/- 0.16; P greater than 0.05) groups there was no variation. Ultrastructural changes in the myocardium were negligible in all three groups at the end of reperfusion. 4. We conclude that intermittent perfusion of a hypothermic cardioplegic solution through the coronary sinus is effective for the protection of the myocardium during total ischemia. Departamento de Cirurgia e Ortopedia Faculdade de Medicina de Botucatu Universidade Estadual Paulista Departamento de Cirurgia e Ortopedia Faculdade de Medicina de Botucatu Universidade Estadual Paulista

Made available in DSpace on 2022-04-28T19:53:47Z (GMT). No. of bitstreams: 0 Previous issue date: 1988-01-01 1. This study analyzes variations of oxygen tension induced by asphyxia in the myocardium (MpO2) and coronary sinus blood (CSpO2) of the anesthetized open-chest dog. Oxygen tension was measured polarographically and arterial blood pressure and coronary blood flow were recorded simultaneously. 2. Asphyxia increased arterial pressure and coronary blood flow but decreased both MpO2 and CSpO2. 3. During the reoxygenation phase, arterial pressure and coronary flow decreased gradually. Reoxygenation induced a rapid but transient increase in CSpO2 above control levels. In contrast, MpO2 returned gradually to the basal state. 4. The mechanism(s) involved in the differential effects of asphyxia followed by reoxygenation on MpO2 and CSpO2 remain to be elucidated. Departamento de Clínica Médica Faculdade de Medicina de Botucatu Universidade Estadual de São Paulo Departamento de Clínica Médica Faculdade de Medicina de Botucatu Universidade Estadual de São Paulo

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2014-02-26T17:05:54Z No. of bitstreams: 0 Made available in DSpace on 2014-02-26T17:05:54Z (GMT). No. of bitstreams: 0 Previous issue date: 1994-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:21:10Z No. of bitstreams: 0 Made available in DSpace on 2014-05-20T15:21:10Z (GMT). No. of bitstreams: 0 Previous issue date: 1994-01-01 Para a avaliação dos parâmetros etológicos, durante 24 horas ininterruptas, a cada 30 dias, em um período de 90 dias de confinamento, 30 animais – 15 da raça Canchim e 15 Nelore – foram distribuídos nos seguintes tratamentos: água (A), vinhaça + óxido de magnésio (V) e vinhaça + óxido de magnésio + bicarbonato de sódio (V+B). Por ocasião das pesagens a cada 28 dias, coletaram-se amostras de sangue para determinação do perfil metabólico. Verificou-se que os tratamentos não influenciaram (P > 0,05) o comportamento animal nem o perfil metabólico, estando os valores médios obtidos dentro dos intervalos de normalidade, sendo para Canchim e Nelore, respectivamente: Alimentação - 270 e 223 min; Ruminação - 374 e 356 min; Ócio - 745 e 863; Glicose - 86 e 88 mg/dl; Uréia -10 e 13 mg/dl; Ácido Úrico -1,3 e 1,2 mg/dl; Proteínas totais - 7,0 e 6,8 g/dl; Albumina - 3,1 e 3,1 g/dl; Creatinina - 1,5 e 1,6 mg/dl; Sódio - 140 e 142 meq/dl; Potássio - 4,2 e 4,3 meq/1 e Cálcio 10,0 e 9,8 mg/dl. Concluíu-se que: A vinhaça líquida em substituição à água não alterou os parâmetros etológico e perfil metabólico dos bovinos dos grupos genéticos, Nelore e Canchim e fase de terminação em confinamento. Fifteen animals from Canchim group and fifteen from Nelore were observed in the following treatments: water (A), molasses soluble + magnesium oxide (V) and molasses soluble + magnesium oxide + sodium bicarbonate (V+B). They were observed during continuous 24 hours every 30 days up to 90 days periods. During the weighting, every 28 days blood samples were collected to determine metabolic outline. Treatments did not affect (P>.05) animal behaviour on metabolic and the obtained mean values were within the normal range. They were, respectively for Canchim and Nelore: Feeding 270 and 223 minutes; Rumination 374 and 356 minutes; Idleness 745 and 863 minutes; Glicose 86 and 88 mg/dl; Urea 10 and 23 mg/dl: Uric acid 1,3 and 1,2 mg/dl; Total protein 7,0 and 6,8 g/dl; Albumin 3,1 and 3,1 g/dl; Creatinin 1,5 and 1,6 mg/dl; Sodium 140 and 142 meq/dl; Potassium 4,2 and 4,3 meq/1 and Calcium 10,0 and 9,8 mg/dl. It was concluded that molasses soluble as a substitute of water has not changed the ethologic parameters neither metabolic outline of both genetic group when in finishing feedlot. UNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,FMVZ,CAMPUS BOTUCATU,CAIXA POSTAL 502,BR-18618000 BOTUCATU,SP,BRAZIL UNIV ESTADUAL PAULISTA JULIO MESQUITA FILHO,FMVZ,CAMPUS BOTUCATU,CAIXA POSTAL 502,BR-18618000 BOTUCATU,SP,BRAZIL

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2014-02-26T17:05:43Z No. of bitstreams: 0 Made available in DSpace on 2014-02-26T17:05:43Z (GMT). No. of bitstreams: 0 Previous issue date: 1993-11-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:28:16Z No. of bitstreams: 0 Made available in DSpace on 2014-05-20T15:28:16Z (GMT). No. of bitstreams: 0 Previous issue date: 1993-11-01 O presente estudo teve por objetivo avaliar o desempenho de bovinos em fase de terminação em confinamento, recebendo vinhaça em substituição à água. Foram utilizados 243 bovinos de dois grupos genéticos, Canchim e Nelore, em um período experimental de 90 dias, com pesagens a cada 28 dias. O delineamento experimental foi o inteiramente aleatorizado, em um fatorial 2 x 3, sendo utilizadas duas raças - Nelore (Nel) e Canchim (Can) - e três tratamentos: água (A), vinhaça + óxido de magnésio (V) e vinhaça + óxido de magnésio + bicarbonato de sódio (V + B). A dieta para todos os tratamentos era composta de 70% de silagem de milho e 30% de cana-de-açúcar picada, ad libitum, acrescida de 1 kg e quirera de milho e 1,5 kg de levedura. Independentemente dos tratamentos, os animais Can revelaram maior ganho de peso (p > 0,01) em relação aos Nel, com 1,35 e 1,22 kg/cab/dia, respectivamente. Considerando-se os tratamentos, ambas as raças apresentaram maior ganho de peso (p > 0,01) no V + B, com 1,44 e 1,32 kg/cab/dia, para Can e Nel, respectivamente, em comparação aos demais: Can (A) = 1,30; Can (V) = 1,31; Nel (A) = 1,16 e Nel (V) = 1,20 kg/cab/dia. O tratamento V + B proporcionou ainda, uma tendência (0,05 < p < 0,10) de melhor conversão alimentar, o que evidencia o efeito positivo da vinhaça, quando associada aos compostos neutralizadores do pH ruminal. During 90 days 243 finishing feedlot animals from two genetic groups, were weighed every 28 days in a randomized experimental procedure in a 2 x 3 factorial arrangement, with two breeds - Nelore (Nel) and Canchim (Can) - and three treatments: water (A), cane stillage + magnesium oxide (V) and cane stillage + magnesium oxide + sodium bicarbonate (V + B). The diet for all treatments was composed of 70% of corn silage and 30% of chopped sugar cane ad libitum plus 1 kg of ground corn and 1,5 kg of soluble yeast per animal. Results showed higher weight gain (P < 0,01) of the Can group relation to those of Nel, having respectively 1,35 and 1,22 kg/head/day. Both Can and Nel groups showed higher weight gain (P

Made available in DSpace on 2022-04-29T08:42:19Z (GMT). No. of bitstreams: 0 Previous issue date: 1988-01-01 Department of Surgery and Orthopaedics, Faculdade de Medicina de Botucatu, Universidade Estadual Paulista, 18610 Botucatu, Sao Paulo