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1. Flow-S: A Field-Deployable Device with Minimal Hands-On Effort to Concentrate and Quantify Schistosoma Circulating Anodic Antigen (CAA) from Large Urine Volumes

Author

de Jong, Daniëlle, primary, Carrell, Cody, additional, Maganga, Jane K., additional, Mhango, Loyce, additional, Shigella, Peter S., additional, Gill, Maddy, additional, Shogren, Ryan, additional, Mullins, Brianna, additional, Warrick, Jay W., additional, Changalucha, John M., additional, van Dam, Govert J., additional, Pham, Khanh, additional, Downs, Jennifer A., additional, and Corstjens, Paul L. A. M., additional

Published
2024
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2. Clinical and demographic factors associated with Kaposi’s sarcoma-associated herpesvirus shedding in saliva or cervical secretions in a cohort of Tanzanian women

Author

Mertelsmann, Anna M, primary, Mukerebe, Crispin, additional, Miyaye, Donald, additional, Shigella, Peter, additional, Mhango, Loyce, additional, Lutonja, Peter, additional, Corstjens, Paul L A M, additional, de Dood, Claudia, additional, van Dam, Govert J, additional, Colombe, Soledad, additional, Maganga, Jane, additional, Aristide, Christine, additional, Kalluvya, Samuel E, additional, Ward, Maureen M, additional, Cordeiro, Alexandra A, additional, Lee, Myung Hee, additional, Changalucha, John M, additional, and Downs, Jennifer A, additional

Published
2024
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3. Schistosome infection among pregnant women in the rural highlands of Madagascar: A cross-sectional study calling for public health interventions in vulnerable populations.

Author

Rakotozandrindrainy, Raphäel, Rakotoarivelo, Rivo Andry, Kislaya, Irina, Marchese, Valentina, Rasamoelina, Tahimandranto, Solonirina, Jeannine, Ratiaharison, Elveric Fesia, Razafindrakoto, Ravo, Razafindralava, Nantenaina Matthieu, Rakotozandrindrainy, Njary, Radomanana, Mickael, Andrianarivelo, Mala Rakoto, Klein, Philipp, Lorenz, Eva, Jaeger, Anna, Hoekstra, Pytsje T., Corstjens, Paul L. A. M., Schwarz, Norbert Georg, van Dam, Govert J., and May, Jürgen

Subjects
PREGNANT women, RURAL women, PARASITIC diseases, PUBLIC health, CROSS-sectional method
Abstract

Introduction: Schistosomiasis is a parasitic infection highly prevalent in sub-Saharan Africa (SSA) with Madagascar being among the countries with highest burden of the disease worldwide. Despite WHO recommendations, suggesting treatment of pregnant women after the first trimester, this group is still excluded from Mass Drug Administration programs. Our study, had the objective to measure the prevalence of schistosome infection among pregnant women in Madagascar in order to inform public health policies for treatment in this vulnerable population. Methods: Women were recruited for this cross-sectional study between April 2019 and February 2020 when attending Antenatal Care Services (ANCs) at one of 42 included Primary Health Care Centers. The urine-based upconverting reporter particle, lateral flow (UCP-LF) test detecting circulating anodic antigen was used for the detection of schistosome infections. To identify factors associated with the prevalence of schistosome infection crude and adjusted prevalence ratios and 95% CIs were estimated using mixed-effect Poisson regression. Results: Among 4,448 participating women aged between 16 and 47 years, the majority (70.4%, 38 n = 3,133) resided in rural settings. Overall, the prevalence of schistosome infection was 55.9% (n = 2486, CI 95%: 53.3–58.5). A statistically significant association was found with age group (increased prevalence in 31–47 years old, compared to 16–20 years old (aPR = 1.15, CI 95%: 1.02–1.29) and with uptake of antimalaria preventive treatment (decreased prevalence, aPR = 0.85, CI 95%: 0.77–0.95). No other associations of any personal characteristics or contextual factors with schistosome infection were found in our multivariate regression analysis. Discussion and conclusion: The high prevalence of schistosome infection in pregnant women supports the consideration of preventive schistosomiasis treatment in ANCs of the Malagasy highlands. We strongly advocate for adapting schistosomiasis programs in highly endemic contexts. This, would contribute to both the WHO and SDGs agendas overall to improving the well-being of women and consequently breaking the vicious cycle of poverty perpetuated by schistosomiasis. Author summary: Schistosomiasis is a parasitic infection highly prevalent in sub-Saharan Africa and in Madagascar, where pregnant women are systematically excluded from prevention and control strategies. Our study shows the urgency for adapting public health strategies. We report a high prevalence of schistosome infection among pregnant women in Madagascar. The lack of systematic treatment for this vulnerable group can have a direct impact on the well-being of women and their offspring contributing to the vicious cycle of poverty perpetuated by schistosomiasis. [ABSTRACT FROM AUTHOR]

Published
2024
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4. A cluster randomized controlled trial for assessing POC-CCA test based praziquantel treatment for schistosomiasis control in pregnant women and their young children: study protocol of the freeBILy clinical trial in Madagascar

Author

Fusco, Daniela, Rakotozandrindrainy, Raphäel, Rakotoarivelo, Rivo Andry, Andrianarivelo, Mala Rakoto, Rakotozandrindrainy, Njary, Rasamoelina, Tahinamandranto, Puradiredja, Dewi Ismajani, Klein, Philipp, Stahlberg, Karl, Dechenaud, Marie, Lorenz, Eva, Jaeger, Anna, Kreidenweiss, Andrea, Hoekstra, Pytsje T., Adegnika, Akim Ayola, Sicuri, Elisa, Corstjens, Paul L. A. M., van Dam, Govert J., May, Jürgen, and Schwarz, Norbert Georg

Published
2021
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5. Rhesus macaques self-curing from a schistosome infection can display complete immunity to challenge

Author

Amaral, Murilo Sena, Santos, Daisy Woellner, Pereira, Adriana S. A., Tahira, Ana Carolina, Malvezzi, João V. M., Miyasato, Patrícia Aoki, Freitas, Rafaela de Paula, Kalil, Jorge, Tjon Kon Fat, Elisa M., de Dood, Claudia J., Corstjens, Paul L. A. M., van Dam, Govert J., Nakano, Eliana, Castro, Simone de Oliveira, Mattaraia, Vânia Gomes de Moura, Augusto, Ronaldo de Carvalho, Grunau, Christoph, Wilson, R. Alan, and Verjovski-Almeida, Sergio

Published
2021
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6. A controlled human Schistosoma mansoni infection model to advance novel drugs, vaccines and diagnostics

Author

Langenberg, Marijke C. C., Hoogerwerf, Marie-Astrid, Koopman, Jan Pieter R., Janse, Jacqueline J., Kos-van Oosterhoud, Janneke, Feijt, Carola, Jochems, Simon P., de Dood, Claudia J., van Schuijlenburg, Roos, Ozir-Fazalalikhan, Arifa, Manurung, Mikhael D., Sartono, Erliyani, van der Beek, Martha T., Winkel, Béatrice M. F., Verbeek-Menken, Petra H., Stam, Koen A., van Leeuwen, Fijs W. B., Meij, Pauline, van Diepen, Angela, van Lieshout, Lisette, van Dam, Govert J., Corstjens, Paul L. A. M., Hokke, Cornelis H., Yazdanbakhsh, Maria, Visser, Leo G., and Roestenberg, Meta

Published
2020
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7. Validation of artificial intelligence-based digital microscopy for automated detection of Schistosoma haematobium eggs in urine in Gabon.

Author

Meulah, Brice, Oyibo, Prosper, Hoekstra, Pytsje T., Moure, Paul Alvyn Nguema, Maloum, Moustapha Nzamba, Laclong-Lontchi, Romeo Aime, Honkpehedji, Yabo Josiane, Bengtson, Michel, Hokke, Cornelis, Corstjens, Paul L. A. M., Agbana, Temitope, Diehl, Jan Carel, Adegnika, Ayola Akim, and van Lieshout, Lisette

Subjects
ARTIFICIAL intelligence, SCHISTOSOMA haematobium, RESOURCE-limited settings, MICROSCOPY, URINE, SPUTUM examination
Abstract

Introduction: Schistosomiasis is a significant public health concern, especially in Sub-Saharan Africa. Conventional microscopy is the standard diagnostic method in resource-limited settings, but with limitations, such as the need for expert microscopists. An automated digital microscope with artificial intelligence (Schistoscope), offers a potential solution. This field study aimed to validate the diagnostic performance of the Schistoscope for detecting and quantifying Schistosoma haematobium eggs in urine compared to conventional microscopy and to a composite reference standard (CRS) consisting of real-time PCR and the up-converting particle (UCP) lateral flow (LF) test for the detection of schistosome circulating anodic antigen (CAA). Methods: Based on a non-inferiority concept, the Schistoscope was evaluated in two parts: study A, consisting of 339 freshly collected urine samples and study B, consisting of 798 fresh urine samples that were also banked as slides for analysis with the Schistoscope. In both studies, the Schistoscope, conventional microscopy, real-time PCR and UCP-LF CAA were performed and samples with all the diagnostic test results were included in the analysis. All diagnostic procedures were performed in a laboratory located in a rural area of Gabon, endemic for S. haematobium. Results: In study A and B, the Schistoscope demonstrated a sensitivity of 83.1% and 96.3% compared to conventional microscopy, and 62.9% and 78.0% compared to the CRS. The sensitivity of conventional microscopy in study A and B compared to the CRS was 61.9% and 75.2%, respectively, comparable to the Schistoscope. The specificity of the Schistoscope in study A (78.8%) was significantly lower than that of conventional microscopy (96.4%) based on the CRS but comparable in study B (90.9% and 98.0%, respectively). Conclusion: Overall, the performance of the Schistoscope was non-inferior to conventional microscopy with a comparable sensitivity, although the specificity varied. The Schistoscope shows promising diagnostic accuracy, particularly for samples with moderate to higher infection intensities as well as for banked sample slides, highlighting the potential for retrospective analysis in resource-limited settings. Trial registration: NCT04505046 ClinicalTrials.gov. Author summary: Assessment of schistosomiasis control programs is a crucial step to understanding the success rate of these control programs. The Schistoscope: an AI-powered automated digital microscope could overcome the limitations of conventional microscopy in endemic resource limited settings as well as in settings lacking microscopy experts. In this study, we carried out an extensive validation of the Schistoscope's diagnostic performance for diagnosis of urogenital schistosomiasis compared to conventional microscopy as well as more accurate diagnostic tests such as real-time PCR and the up-converting particle (UCP) lateral flow (LF) test for the detection of circulating anodic antigen (CAA) on freshly collected urines. We also assessed the performance of the Schistoscope for the diagnosis of schistosomiasis on banked sample slides, using a simple and sustainable storage method, for approximately two years. Having a tool that can prospectively and retrospectively analyse samples in an easy and sustainable way could facilitate schistosomiasis control programs in settings with little or no access to microscopists. Overall, we found the Schistoscope to be as good as conventional microscopy for the diagnosis of schistosomiasis, and given its downstream advantages of digital health, it would serve as a valuable diagnostic/screening tool in resource limited endemic settings. [ABSTRACT FROM AUTHOR]

Published
2024
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8. Diagnostic performance of host protein signatures as a triage test for active pulmonary TB

Author

Koeppel, Lisa, primary, Denkinger, Claudia M., additional, Wyss, Romain, additional, Broger, Tobias, additional, Chegou, Novel N., additional, Dunty, Jill M., additional, Scott, Kerry, additional, Cáceres, Tatiana, additional, Dutoit, Elloise, additional, Ugarte-Gil, Cesar, additional, Nicol, Mark, additional, Gotuzzo, Eduardo, additional, Corstjens, Paul L. A. M., additional, Geluk, Annemieke, additional, Sutherland, Jayne, additional, Sigal, George B., additional, Moreau, Emmanuel, additional, Albertini, Audrey, additional, Mantsoki, Anna, additional, Ongarello, Stefano, additional, Walzl, Gerhard, additional, and Fernandez Suarez, Marta, additional

Published
2023
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10. Establishing a Single-Sex Controlled Human Schistosoma mansoni Infection Model for Uganda: Protocol for Safety and Dose-Finding Trial

Author

Abaasa, Andrew, primary, Egesa, Moses, additional, Driciru, Emmanuella, additional, Koopman, Jan Pieter R, additional, Kiyemba, Ronald, additional, Sanya, Richard E, additional, Nassuuna, Jacent, additional, Ssali, Agnes, additional, Kimbugwe, Geofrey, additional, Wajja, Anne, additional, van Dam, Govert J, additional, Corstjens, Paul L A M, additional, Cose, Stephen, additional, Seeley, Janet, additional, Kamuya, Dorcas, additional, Webb, Emily L, additional, Yazdanbakhsh, Maria, additional, Kaleebu, Pontiano, additional, Siddiqui, Afzal A, additional, Kabatereine, Narcis, additional, Tukahebwa, Edridah, additional, Roestenberg, Meta, additional, and Elliott, Alison M, additional

Published
2023
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11. Schistosoma mansoni infection alters the host pre-vaccination environment resulting in blunted Hepatitis B vaccination immune responses

Author

Muir, Roshell, primary, Metcalf, Talibah, additional, Fourati, Slim, additional, Bartsch, Yannic, additional, Kyosiimire-Lugemwa, Jacqueline, additional, Canderan, Glenda, additional, Alter, Galit, additional, Muyanja, Enoch, additional, Okech, Brenda, additional, Namatovu, Teddy, additional, Namara, Irene, additional, Namuniina, Annemarie, additional, Ssetaala, Ali, additional, Mpendo, Juliet, additional, Nanvubya, Annet, additional, Kitandwe, Paul Kato, additional, Bagaya, Bernard S., additional, Kiwanuka, Noah, additional, Nassuna, Jacent, additional, Biribawa, Victoria Menya, additional, Elliott, Alison M., additional, de Dood, Claudia J., additional, Senyonga, William, additional, Balungi, Priscilla, additional, Kaleebu, Pontiano, additional, Mayanja, Yunia, additional, Odongo, Matthew, additional, Connors, Jennifer, additional, Fast, Pat, additional, Price, Matt A., additional, Corstjens, Paul L. A. M., additional, van Dam, Govert J., additional, Kamali, Anatoli, additional, Sekaly, Rafick Pierre, additional, and Haddad, Elias K., additional

Published
2023
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12. Sensitivity and specificity of human point-of-care circulating cathodic antigen (POC-CCA) test in African livestock for rapid diagnosis of schistosomiasis: A Bayesian latent class analysis

Author

Calvo-Urbano, Beatriz, primary, Léger, Elsa, additional, Gabain, Isobel, additional, De Dood, Claudia J., additional, Diouf, Nicolas D., additional, Borlase, Anna, additional, Rudge, James W., additional, Corstjens, Paul L. A. M., additional, Sène, Mariama, additional, Van Dam, Govert J., additional, Walker, Martin, additional, and Webster, Joanne P., additional

Published
2023
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15. Association of schistosome infection with adiposity in Tanzania

Author

Pham, Khanh, primary, PrayGod, George, additional, Faurholt-Jepsen, Daniel, additional, Olsen, Mette F., additional, Kavishe, Bazil, additional, Kitilya, Brenda, additional, Corstjens, Paul L. A. M., additional, de Dood, Claudia J., additional, Friis, Henrik, additional, Filteau, Suzanne, additional, Downs, Jennifer A., additional, and Peck, Robert N., additional

Published
2023
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19. Limited efficacy of repeated praziquantel treatment in Schistosoma mansoni infections as revealed by highly accurate diagnostics, PCR and UCP-LF CAA (RePST trial)

Author

Hoekstra, Pytsje T., primary, Casacuberta-Partal, Miriam, additional, van Lieshout, Lisette, additional, Corstjens, Paul L. A. M., additional, Tsonaka, Roula, additional, Assaré, Rufin K., additional, Silué, Kigbafori D., additional, N’Goran, Eliézer K., additional, N’Gbesso, Yves K., additional, Brienen, Eric A. T., additional, Roestenberg, Meta, additional, Knopp, Stefanie, additional, Utzinger, Jürg, additional, Coulibaly, Jean T., additional, and van Dam, Govert J., additional

Published
2022
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20. The Presence of Hemoglobin in Cervicovaginal Lavage Is Not Associated With Genital Schistosomiasis in Zambian Women From the BILHIV Study

Author

Sturt, Amy S, primary, Webb, Emily L, additional, Phiri, Comfort R, additional, Mapani, Joyce, additional, Mudenda, Maina, additional, Himschoot, Lisa, additional, Kjetland, Eyrun F, additional, Mweene, Tobias, additional, Levecke, Bruno, additional, van Dam, Govert J, additional, Corstjens, Paul L A M, additional, Ayles, Helen, additional, Hayes, Richard J, additional, Francis, Suzanna C, additional, van Lieshout, Lisette, additional, Cools, Piet, additional, Hansingo, Isaiah, additional, and Bustinduy, Amaya L, additional

Published
2022
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21. Schistosoma mansoni Infection Is Associated With Increased Monocytes and Fewer Natural Killer T Cells in the Female Genital Tract

Author

Kingery, Justin R, primary, Chalem, Andrea, additional, Mukerebe, Crispin, additional, Shigella, Peter S, additional, Miyaye, Donald, additional, Magawa, Ruth G, additional, Ward, Maureen, additional, Kalluvya, Samuel E, additional, McCormick, Jason, additional, Maganga, Jane K, additional, Colombe, Soledad, additional, Aristide, Christine, additional, Corstjens, Paul L A M, additional, Lee, Myung Hee, additional, Changalucha, John M, additional, and Downs, Jennifer A, additional

Published
2022
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22. Diagnosis of Schistosomiasis without a Microscope: Evaluating Circulating Antigen (CCA, CAA) and DNA Detection Methods on Banked Samples of a Community-Based Survey from DR Congo

Author

Hoekstra, Pytsje T., primary, Madinga, Joule, additional, Lutumba, Pascal, additional, van Grootveld, Rebecca, additional, Brienen, Eric A. T., additional, Corstjens, Paul L. A. M., additional, van Dam, Govert J., additional, Polman, Katja, additional, and van Lieshout, Lisette, additional

Published
2022
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23. Associations between schistosomiasis and HIV‐1 acquisition risk in four prospective cohorts: a nested case‐control analysis

Author

Bochner, Aaron F., Baeten, Jared M., Secor, W Evan, Dam, Govert J., Szpiro, Adam A., Njenga, Sammy M., Corstjens, Paul L A M., Newsam, Austin, Mugo, Nelly R., Celum, Connie, Mujugira, Andrew, Mcclelland, R Scott, and Barnabas, Ruanne V.

Subjects
Schistosomiasis -- Physiological aspects, AIDS (Disease) -- Research, AIDS research, Host-parasite relationships, HIV infection -- Risk factors, Health
Abstract

: Introduction: Globally, schistosomes infect approximately 200 million people, with 90% of infections in sub‐Saharan Africa. Schistosomiasis is hypothesized to increase HIV‐1 acquisition risk, and multiple cross‐sectional studies reported strong associations. We evaluated this hypothesis within four large prospective cohorts. Methods: We conducted nested case‐control analyses within three longitudinal cohorts of heterosexual HIV‐1 serodiscordant couples and one female sex worker (FSW) cohort from Kenya and Uganda. The serodiscordant couples studies were conducted between 2004 and 2012 while the FSW cohort analysis included participant follow‐up from 1993 to 2014. Cases HIV‐1 seroconverted during prospective follow‐up; three controls were selected per case. The presence of circulating anodic antigen in archived serum, collected prior to HIV‐1 seroconversion, identified participants with active schistosomiasis; immunoblots determined the schistosome species. Data from serodiscordant couples cohorts were pooled, while the FSW cohort was analysed separately to permit appropriate confounder adjustment. Results: We included 245 HIV‐1 seroconverters and 713 controls from the serodiscordant couples cohorts and 330 HIV‐1 seroconverters and 962 controls from the FSW cohort. The prevalence of active schistosomiasis was 20% among serodiscordant couples and 22% among FSWs. We found no association between schistosomiasis and HIV‐1 acquisition risk among males (adjusted odds ratio (aOR) = 0.99, 95% CI 0.59 to 1.67) or females (aOR = 1.21, 95% CI 0.64 to 2.30) in serodiscordant couples. Similarly, in the FSW cohort we detected no association (adjusted incidence rate ratio (aIRR) = 1.11, 95% CI 0.83 to 1.50). Exploring schistosome species‐specific effects, there was no statistically significant association between HIV‐1 acquisition risk and Schistosoma mansoni (serodiscordant couples: aOR = 0.90, 95% CI 0.56 to 1.44; FSW: aIRR = 0.83, 95% CI 0.53 to 1.20) or Schistosoma haematobium (serodiscordant couples: aOR = 1.06, 95% CI 0.46 to 2.40; FSW: aIRR = 1.64, 95% CI 0.93 to 2.87) infection. Conclusions: Schistosomiasis was not a strong risk factor for HIV‐1 acquisition in these four prospective studies. S. mansoni was responsible for the majority of schistosomiasis in these cohorts, and our results do not support the hypothesis that S. mansoni infection is associated with increased HIV‐1 acquisition risk. S. haematobium infection was associated with a point estimate of elevated HIV‐1 risk in the FSW cohort that was not statistically significant, and there was no trend towards a positive association in the serodiscordant couples cohorts., Introduction Schistosomiasis, a parasitic disease caused by the schistosome flatworm, affects approximately 200 million people globally [1], over 90% of whom live in sub‐Saharan Africa [2,3]. In Africa, schistosomiasis is [...]

Published
2020
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25. Sensitive Diagnosis and Post-Treatment Follow-Up of Schistosoma mansoni Infections in Asymptomatic Eritrean Refugees by Circulating Anodic Antigen Detection and Polymerase Chain Reaction

Author

Hoekstra, Pytsje T., primary, Chernet, Afona, additional, de Dood, Claudia J., additional, Brienen, Eric A. T., additional, Corstjens, Paul L. A. M., additional, Labhardt, Niklaus D., additional, Nickel, Beatrice, additional, Wammes, Linda J., additional, van Dam, Govert J., additional, Neumayr, Andreas, additional, and van Lieshout, Lisette, additional

Published
2022
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26. Female Genital Schistosomiasis Lesions Explored Using Circulating Anodic Antigen as an Indicator for Live Schistosoma Worms

Author

Nemungadi, Takalani Girly, primary, Kleppa, Elisabeth, additional, van Dam, Govert J., additional, Corstjens, Paul L. A. M., additional, Galappaththi-Arachchige, Hashini Nilushika, additional, Pillay, Pavitra, additional, Gundersen, Svein Gunnar, additional, Vennervald, Birgitte J., additional, Ndhlovu, Patricia, additional, Taylor, Myra, additional, Naidoo, Saloshni, additional, and Kjetland, Eyrun F., additional

Published
2022
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27. Characterisation of tetraspanins from Schistosoma haematobium and evaluation of their potential as novel diagnostic markers

Author

Mekonnen, Gebeyaw G., primary, Tedla, Bemnet A., additional, Pearson, Mark S., additional, Becker, Luke, additional, Field, Matt, additional, Amoah, Abena S., additional, van Dam, Govert, additional, Corstjens, Paul L. A. M., additional, Mduluza, Takafira, additional, Mutapi, Francisca, additional, Loukas, Alex, additional, and Sotillo, Javier, additional

Published
2022
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28. Immunomics-guided discovery of serum and urine antibodies for diagnosing urogenital schistosomiasis: a biomarker identification study

Author

Pearson, Mark S, primary, Tedla, Bemnet A, additional, Mekonnen, Gebeyaw G, additional, Proietti, Carla, additional, Becker, Luke, additional, Nakajima, Rie, additional, Jasinskas, Al, additional, Doolan, Denise L, additional, Amoah, Abena S, additional, Knopp, Stefanie, additional, Rollinson, David, additional, Ali, Said M, additional, Kabole, Fatma, additional, Hokke, Cornelis H, additional, Adegnika, Akim A, additional, Field, Matt A, additional, van Dam, Govert, additional, Corstjens, Paul L A M, additional, Mduluza, Takafira, additional, Mutapi, Francisca, additional, Oeuvray, Claude, additional, Greco, Beatrice, additional, Chaiyadet, Sujittra, additional, Laha, Thewarach, additional, Cai, Pengfei, additional, McManus, Donald P, additional, Bottazzi, Maria Elena, additional, Felgner, Philip L, additional, Sotillo, Javier, additional, and Loukas, Alex, additional

Published
2021
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29. Detection and Monitoring of Mycobacterium leprae Infection in Nine Banded Armadillos (Dasypus novemcinctus) Using a Quantitative Rapid Test

Author

Zhou, Zijie, primary, Pena, Maria, additional, van Hooij, Anouk, additional, Pierneef, Louise, additional, de Jong, Danielle, additional, Stevenson, Roena, additional, Walley, Rachel, additional, Corstjens, Paul L. A. M., additional, Truman, Richard, additional, Adams, Linda, additional, and Geluk, Annemieke, additional

Published
2021
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30. Accuracy of Two Circulating Antigen Tests for the Diagnosis and Surveillance of Schistosoma mansoni Infection in Low-Endemicity Settings of Côte d’Ivoire

Author

Assaré, Rufin K., primary, Tra-Bi, Mathieu I., additional, Coulibaly, Jean T., additional, Corstjens, Paul L. A. M., additional, Ouattara, Mamadou, additional, Hürlimann, Eveline, additional, van Dam, Govert J., additional, Utzinger, Jürg, additional, and N’Goran, Eliézer K., additional

Published
2021
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31. Association of Female Genital Schistosomiasis With the Cervicovaginal Microbiota and Sexually Transmitted Infections in Zambian Women

Author

Sturt, Amy S, primary, Webb, Emily L, additional, Himschoot, Lisa, additional, Phiri, Comfort R, additional, Mapani, Joyce, additional, Mudenda, Maina, additional, Kjetland, Eyrun F, additional, Mweene, Tobias, additional, Levecke, Bruno, additional, van Dam, Govert J, additional, Corstjens, Paul L A M, additional, Ayles, Helen, additional, Hayes, Richard J, additional, van Lieshout, Lisette, additional, Hansingo, Isaiah, additional, Francis, Suzanna C, additional, Cools, Piet, additional, and Bustinduy, Amaya L, additional

Published
2021
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32. Sensitive diagnosis and post-treatment follow-up of Schistosoma mansoni infections in asymptomatic Eritrean refugees by Circulating Anodic Antigen (CAA) detection and PCR

Author

Hoekstra, Pytsje T., Chernet, Afona, de Dood, Claudia J., Brienen, Eric A. T., Corstjens, Paul L. A. M., Labhardt, Niklaus D., Nickel, Beatrice, Wammes, Linda, van Dam, Govert J., Neumayr, Andreas, and van Lieshout, Lisette

Abstract

The increasing number of refugees coming from or passing through Schistosoma-endemic areas and arriving in Europe highlights the importance of screening for schistosomiasis on arrival, and focuses attention on the choice of diagnostic test. We evaluate the diagnostic performance of circulating anodic antigen (CAA) detection in 92 asymptomatic refugees from Eritrea. Results were compared with already-available stool microscopy, serology, and urine point-of-care circulating cathodic antigen (POC-CCA) data. For a full diagnostic comparison, real-time polymerase chain reaction (PCR) and the POC-CCA were included. All outcomes were compared against a composite reference standard. Urine and serum samples were subjected to the ultra-sensitive and highly specific up-converting particle lateral flow CAA test, Schistosoma spp. real-time PCR was performed on urine and stool, and the POC-CCA was used on urine using the G-score method. CAA was detected in 43% of urine and in 40% of serum samples. Urine PCR was negative in all 92 individuals, whereas 25% showed Schistosoma DNA in stool. POC-CCA was positive in 30% of individuals. The CAA test confirmed all microscopy positives, except for two cases that were also negative by all other diagnostic procedures. Post-treatment, a significant reduction in the number of positives and infection intensity was observed, in particular regarding CAA levels. Our findings confirm that microscopy, serology, and POC-CCA lack the sensitivity to detect all active Schistosoma infections. Accuracy of stool PCR was similar to microscopy, indicating that this method also lacks sensitivity. The CAA test appeared to be the most accurate method for screening active Schistosoma infections and for monitoring treatment efficacy.

Published
2021

33. High prevalence of Schistosoma mansoni infection and stunting among school age children in communities along the Albert-Nile, Northern Uganda: A cross sectional study.

Author

Mulindwa, Julius, Namulondo, Joyce, Kitibwa, Anna, Nassuuna, Jacent, Nyangiri, Oscar Asanya, Kimuda, Magambo Phillip, Boobo, Alex, Nerima, Barbara, Busingye, Fred, Candia, Rowel, Namukuta, Annet, Ssenyonga, Ronald, Ukumu, Noah, Ajal, Paul, Adriko, Moses, Noyes, Harry, de Dood, Claudia J., Corstjens, Paul L. A. M., van Dam, Govert J., and Elliott, Alison M.

Subjects
STUNTED growth, SCHOOL children, SCHISTOSOMA mansoni, SHORELINES, CHILD nutrition, FRESH water, GIRLS
Abstract

Background: Knowing the prevalence of schistosomiasis is key to informing programmes to control and eliminate the disease as a public health problem. It is also important to understand the impact of infection on child growth and development in order to allocate appropriate resources and effort to the control of the disease. Methods: We conducted a survey to estimate the prevalence of schistosomiasis among school aged children in villages along the Albert-Nile shore line in the district of Pakwach, North Western Uganda. A total of 914 children aged between 10–15 years were screened for Schistosoma mansoni using the POC-CCA and Kato Katz (KK) techniques. The infection intensities were assessed by POC-CCA and KK as well as CAA tests. The KK intensities were also correlated with POC-CCA and with CAA intensity. Anthropometric measurements were also taken and multivariate analysis was carried out to investigate their association with infection status. Results: The prevalence of schistosomiasis using the POC-CCA diagnostic test was estimated at 85% (95% CI: 83–87), being highest amongst children living closer to the Albert-Nile shoreline. Visual scoring of the POC-CCA results was more sensitive than the Kato Katz test and was positively correlated with the quantified infection intensities by the CAA test. The majority of the children were underweight (BMI<18.5), and most notably, boys had significantly lower height for age (stunting) than girls in the same age range (p < 0.0001), but this was not directly associated with S. mansoni infection. Conclusion: High prevalence of S. mansoni infection in the region calls for more frequent mass drug administration with praziquantel. We observed high levels of stunting which was not associated with schistosomiasis. There is a need for improved nutrition among the children in the area. Author summary: Schistosomiasis is a neglected but frequent disease that is caused by schistosomes, with over 290 million people worldwide at risk of infection. The major mode of transmission is through contact with fresh water sources infested with infected snails (the intermediate host). In this study, using the point of care test (POC-CCA), we screened 914 school aged children (10–15 years) living in the rural communities along the Lake Albert- River Nile shores of Pakwach district in Northern Uganda. We observed a very high prevalence of S. mansoni infections (over 80%) although the prevalence dropped to 40% in communities that were further from the lake shores. This high prevalence was also coupled with Kato Katz light schistosome infection intensities as categorised by WHO guidelines. We further compared the POC-CCA and Kato Katz tests to the more sensitive CAA assay and this revealed that even though both tests gave good probability of positive prediction, the POC-CCA had higher sensitivity in screening for S. mansoni infections than Kato Katz assay. The study also revealed high levels of stunting within the children, more so amongst boys. Frequent screening and mass treatment of these communities with praziquantel will reduce on the infection rates. But in addition, improved hygiene and sanitation will be required for a sustainable reduction in the prevalence and morbidity of schistosomiasis in the Albert-Nile communities along with dietary intervention for optimal child health. [ABSTRACT FROM AUTHOR]

Published
2022
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35. Female Genital Schistosomiasis and HIV-1 Incidence in Zambian Women: A Retrospective Cohort Study

Author

Sturt, Amy S, primary, Webb, Emily L, additional, Phiri, Comfort R, additional, Mudenda, Maina, additional, Mapani, Joyce, additional, Kosloff, Barry, additional, Cheeba, Maina, additional, Shanaube, Kwame, additional, Bwalya, Justin, additional, Kjetland, Eyrun F, additional, Francis, Suzanna C, additional, Corstjens, Paul L A M, additional, van Dam, Govert J, additional, van Lieshout, Lisette, additional, Hansingo, Isaiah, additional, Ayles, Helen, additional, Hayes, Richard J, additional, and Bustinduy, Amaya L, additional

Published
2021
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36. Cervicovaginal Immune Activation in Zambian Women With Female Genital Schistosomiasis

Author

Sturt, Amy S., primary, Webb, Emily L., additional, Patterson, Catriona, additional, Phiri, Comfort R., additional, Mweene, Tobias, additional, Kjetland, Eyrun F., additional, Mudenda, Maina, additional, Mapani, Joyce, additional, Mutengo, Mable M., additional, Chipeta, James, additional, van Dam, Govert J., additional, Corstjens, Paul L. A. M., additional, Ayles, Helen, additional, Hayes, Richard J., additional, Hansingo, Isaiah, additional, Cools, Piet, additional, van Lieshout, Lisette, additional, Helmby, Helena, additional, McComsey, Grace A., additional, Francis, Suzanna C., additional, and Bustinduy, Amaya L., additional

Published
2021
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37. Genital self-sampling compared with cervicovaginal lavage for the diagnosis of female genital schistosomiasis in Zambian women: The BILHIV study

Author

Sturt, Amy S., Webb, Emily L., Phiri, Comfort R., Mweene, Tobias, Chola, Namakau, van Dam, Govert J., Corstjens, Paul L. A. M., Wessels, Els, Stothard, Russell, Hayes, Richard, Ayles, Helen, Hansingo, Isaiah, van Lieshout, Lisette, and Bustinduy, Amaya L.

Subjects
wj_140, wj_100, wa_395, wa_309, wc_810, wp_141
Abstract

Background: Given the potentially causal association of female genital schistosomiasis (FGS) with HIV-1 infection, improved diagnostics are urgently needed to scale-up FGS surveillance. The BILHIV (bilharzia and HIV) study assessed the performance of home-based self-collection methods (cervical and vaginal swabs) compared to cervicovaginal lavage (CVL) for the detection of Schistosoma DNA by real-time polymerase chain reaction (PCR). Methods: Between January and August 2018, a consecutive series of female participants from the Population-Cohort of the previous HIV prevention trial HPTN 071 (PopART), resident in Livingstone, Zambia were invited to take part in BILHIV if they were 18–31 years old, non-pregnant and sexually active. Genital self-collected swabs and a urine specimen were obtained and a questionnaire completed at home visits. CVL was obtained at clinic follow-up. Results: 603 women self-collected genital swabs. Of these, 527 women had CVL performed by a mid-wife during clinic follow-up. Schistosoma DNA was more frequently detected in genital self-collected specimens (24/603, 4.0%) compared to CVL (14/527, 2.7%). Overall, 5.0% (30/603) women had female genital schistosomiasis, defined as a positive PCR by any genital sampling method (cervical swab PCR, vaginal swab PCR, or CVL PCR) and 95% (573/603) did not have a positive genital PCR. The sensitivity of any positive genital self-collected swab against CVL was 57.1% (95% CI 28.9–82.3%), specificity 97.3% (95.5–98.5%). In a subset of participants with active schistosome infection, determined by detectable urine Circulating Anodic Antigen (CAA) (15.1%, 91/601), positive PCR (4.3%, 26/601), or positive microscopy (5.5%, 33/603), the sensitivity of any positive self-collected specimen against CVL was 88.9% (51.8–99.7%). Conclusions: Genital self-sampling increased the overall number of PCR-based FGS diagnoses in a field setting, compared with CVL. Home-based sampling may represent a scalable alternative method for FGS community-based diagnosis in endemic resource limited settings.

Published
2020

38. Survey and Diagnostic Challenges after Transmission-Stop: Confirming Elimination of Schistosomiasis haematobium in Morocco

Author

Fatima, Amarir, Abdelaali, Balahbib, Corstjens, Paul L. A. M., Abderrahim, Sadak, El Bachir, Adlaoui, and Mohamed, Rhajaoui

Subjects
Article Subject
Abstract

Clinical cases of Moroccan residents have been recorded since 2004, indicating successful interruption of transmission of S. haematobium infection at national level. The first national survey initiated in 2009 for Schistosomiasis haematobium among children born after 2004, applied diagnostic test was the HAMA-EITB, based on the Western blot technology, and molecular malacological diagnostic tools clearly confirm transmission stop. In 2015, a recent, small survey utilizing an HAI, ELISA tests and an ultrasensitive antigen test, FTCUP CAA, in a group of individual with a past history of infection. However, obviously follow-up surveys to prevent reemergency and for certification of the schistosomiasis elimination require vigilant diagnosis strategies. Here we discuss diagnosis story line in the national laboratory and challenges based on the available tools in relation to their clinical parameters (sensitivity/specificity; Sn/Sp), practicability and associated costs. When transmission stop has been achieved, survey cost and speed are likely to benefit from cost effective pooling strategies and ultrasensitive assays indicating active infection in all potential risk groups. Similarly molecular pooling strategies to monitor infections in the snail vectors.

Published
2020
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39. Additional file 1 of Prospective, observational study to assess the performance of CAA measurement as a diagnostic tool for the detection of Schistosoma haematobium infections in pregnant women and their child in Lambaréné, Gabon: study protocol of the freeBILy clinical trial in Gabon

Author

Yabo Josiane Honkpehedji, Ayôla Akim Adegnika, Dejon-Agobe, Jean Claude, Zinsou, Jeannot Fréjus, Mba, Romuald Beh, Gerstenberg, Jacob, Rakotozandrindrainy, Raphaël, Rakotoarivelo, Rivo Andry, Tahinamandranto Rasamoelina, Sicuri, Elisa, Schwarz, Norbert G., Corstjens, Paul L. A. M., Pytsje T. Hoekstra, Dam, Govert J. Van, and Kreidenweiss, Andrea

Abstract

Additional file 1. Overview of study visits over time with respect to the study phase and details of sample collection, describes the study phases and procedures.

Published
2020
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40. The burden of HIV, syphilis and schistosome infection and associated factors among adults in the fishing communities in northwestern Tanzania

Author

Kapiga, Saidi, primary, Hansen, Christian H., additional, Downs, Jennifer A., additional, Sichalwe, Simon, additional, Hashim, Ramadhan, additional, Mngara, Julius, additional, van Dam, Govert J., additional, Corstjens, Paul L. A. M., additional, Kingery, Justin R., additional, Peck, Robert N., additional, and Grosskurth, Heiner, additional

Published
2020
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41. Genital self-sampling compared with cervicovaginal lavage for the diagnosis of female genital schistosomiasis in Zambian women: The BILHIV study

Author

Sturt, Amy S., primary, Webb, Emily L., additional, Phiri, Comfort R., additional, Mweene, Tobias, additional, Chola, Namakau, additional, van Dam, Govert J., additional, Corstjens, Paul L. A. M., additional, Wessels, Els, additional, Stothard, J. Russell, additional, Hayes, Richard, additional, Ayles, Helen, additional, Hansingo, Isaiah, additional, van Lieshout, Lisette, additional, and Bustinduy, Amaya L., additional

Published
2020
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42. Circulating Anodic Antigen (CAA): A Highly Sensitive Diagnostic Biomarker to Detect Active Schistosoma Infections—Improvement and Use during SCORE

Author

Corstjens, Paul L. A. M., primary, de Dood, Claudia J., additional, Knopp, Stefanie, additional, Clements, Michelle N., additional, Ortu, Giuseppina, additional, Umulisa, Irenee, additional, Ruberanziza, Eugene, additional, Wittmann, Udo, additional, Kariuki, Thomas, additional, LoVerde, Philip, additional, Secor, William Evan, additional, Atkins, Lydia, additional, Kinung’hi, Safari, additional, Binder, Sue, additional, Campbell, Carl H., additional, Colley, Daniel G., additional, and van Dam, Govert J., additional

Published
2020
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43. Nationwide Remapping of Schistosoma mansoni Infection in Rwanda Using Circulating Cathodic Antigen Rapid Test: Taking Steps toward Elimination

Author

Ruberanziza, Eugene, primary, Wittmann, Udo, additional, Mbituyumuremyi, Aimable, additional, Mutabazi, Alphonse, additional, Campbell, Carl H., additional, Colley, Daniel G., additional, Fleming, Fiona M., additional, Ortu, Giuseppina, additional, van Dam, Govert J., additional, Umulisa, Irenee, additional, Tallant, Jamie, additional, Kabera, Michee, additional, Semakula, Muhammed, additional, Corstjens, Paul L. A. M., additional, Munyaneza, Tharcisse, additional, Lancaster, Warren, additional, Mbonigaba, Jean Bosco, additional, and Clements, Michelle N., additional

Published
2020
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44. Schistosoma haematobium infection is associated with lower serum cholesterol levels and improved lipid profile in overweight/obese individuals

Author

Zinsou, Jeannot F., primary, Janse, Jacqueline J., additional, Honpkehedji, Yabo Y., additional, Dejon-Agobé, Jean Claude, additional, García-Tardón, Noemí, additional, Hoekstra, Pytsje T., additional, Massinga-Loembe, Marguerite, additional, Corstjens, Paul L. A. M., additional, van Dam, Govert J., additional, Giera, Martin, additional, Kremsner, Peter G., additional, Yazdanbakhsh, Maria, additional, Adegnika, Ayola A., additional, and Guigas, Bruno, additional

Published
2020
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47. Efficacy of single versus four repeated doses of praziquantel against Schistosoma mansoni infection in school-aged children from Côte d'Ivoire based on Kato-Katz and POC-CCA: An open-label, randomised controlled trial (RePST)

Author

Hoekstra, Pytsje T., primary, Casacuberta-Partal, Miriam, additional, van Lieshout, Lisette, additional, Corstjens, Paul L. A. M., additional, Tsonaka, Roula, additional, Assaré, Rufin K., additional, Silué, Kigbafori D., additional, Meité, Aboulaye, additional, N’Goran, Eliézer K., additional, N’Gbesso, Yves K., additional, Amoah, Abena S., additional, Roestenberg, Meta, additional, Knopp, Stefanie, additional, Utzinger, Jürg, additional, Coulibaly, Jean T., additional, and van Dam, Govert J., additional

Published
2020
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48. Schistosomiasis was not associated with higher HIV-1 plasma or genital set point viral loads among HIV seroconverters from four cohort studies

Author

Bochner, Aaron F., primary, Secor, W. Evan, additional, Baeten, Jared M., additional, van Dam, Govert J., additional, Szpiro, Adam A., additional, Njenga, Sammy M., additional, Corstjens, Paul L. A. M., additional, Mackelprang, Romel D., additional, Mugo, Nelly R., additional, Overbaugh, Julie, additional, Celum, Connie, additional, Mujugira, Andrew, additional, McClelland, R. Scott, additional, and Barnabas, Ruanne V., additional

Published
2019
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49. Performance of an ultra-sensitive assay targeting the circulating anodic antigen (CAA) for detection of Schistosoma mansoni Infection in a low endemic area in Brazil

Author

Sousa, Mariana Silva, Dam, Govert J. van, Pinheiro, Marta Cristhiany Cunha, Dood, Claudia J. de, Peralta, Jose Mauro, Peralta, Regina Helena Saramago, Daher, Elizabeth de Francesco, Corstjens, Paul L. A. M, and Bezerra, Fernando Schemelzer Moraes

Subjects
Diagnóstico, Brasil, Diagnosis, Fósforo, Phosphorus, Schistosoma mansoni, Polymerase Chain Reaction, Brazil, Reação em Cadeia da Polimerase
Abstract

Techniques with high sensitivity and specificity are required for an accurate diagnosis in low-transmission settings, where the conventional parasitological methods are insensitive. We determined the accuracy of an up-converting phosphor-lateral flow circulating anodic antigen (UCP-LF CAA) assay in urine and serum for Schistosoma mansoni diagnosis in low-prevalence settings in Ceará, Brazil, before and after praziquantel treatment. Clinical samples of a total of 258 individuals were investigated by UCP-LF CAA, point-of-care—circulating cathodic antigen (POC-CCA), soluble worm antigen preparation (SWAP)-ELISA and Kato-Katz (KK); a selection of 128 stools by real-time PCR technique. Three and 6-weeks after treatment, samples were collected and evaluated by detection Schistosoma circulating antigens (CAA and CCA). The UCP-LF CAA assays detected 80 positives (31%) with urine and 82 positives (31.8%) with serum. The urine POC-CCA and serum SWAP-ELISA assays detected 30 (11.6%) and 107 (40.7%) positives, respectively. The Kato-Katz technique revealed only 4 positive stool samples (1.6%). Among the 128 individuals with complete data records, 19 cases were identified by PCR (14.8%); Sensitivities and specificities of the UCP-LF CAA assays, determined versus a combined reference standard based on CCA/KK/PCR positivity, ranged from60–68%to 68–77%, respectively. In addition only for comparative purposes, sensitivities of the different assays were determined vs. a comparative reference based on CAA/KK/PCR positivity, showing the highest sensitivity for the urine CAA assay (80%), followed by the serum CAA (70.9%), SWAP-ELISA (43.6%), PCR (34.5%), POC-CCA (29.1%), whilst triplicate Kato-Katz thick smears had a very low sensitivity (3.6%). CAA concentrations were higher in serum than in urine and were significantly correlated. There was a significant decrease in urine and serum CAA levels 3 and 6-weeks after treatment. The UCP-LF CAA assays revealed 33 and 28 S. mansoni-infected patients at the 3- and 6-week post-treatment follow-up, respectively. The UCP-LF CAA assays show high sensitivity for the diagnosis of S.mansoni in low-endemicity settings. It detects a considerably higher number of infections than microscopy, POC-CCA or PCR. Also it shows to be very useful for evaluating cure rates after treatment. Hence, the UCP-LF CAA assay is a robust and promising diagnostic approach in low-transmission settings.

Published
2019

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