Search

Your search keyword '"Coppedé, N."' showing total 6 results
Start Over Author "Coppedé, N." Search Limiters Full Text
6 results on '"Coppedé, N."'

4. Transforming diatomaceous earth into sensing devices by surface modification with gold nanoparticles

Author

Valentina Onesto, Immanuel Valpapuram, Edmondo Battista, A. Zappettini, Marco Villani, Andrea Schirato, Maria Laura Coluccio, Roksana Majewska, Francesco Gentile, Nicola Coppedè, E. Di Fabrizio, Alessandro Alabastri, Davide Calestani, Francesco Amato, Villani, M., Onesto, V., Coluccio, M. L., Valpapuram, I., Majewska, R., Alabastri, A., Battista, E., Schirato, A., Calestani, D., Coppedé, N., Zappettini, A., Amato, F., Di Fabrizio, E., Gentile, F., and 29675146 - Majewska, Roksana

Subjects
Diatomite, Materials science, Gold nanoparticle, Nanoparticle, lcsh:TK7800-8360, symbols.namesake, lcsh:Technology (General), Gold nanoparticles, Photo-deposition, Electrical and Electronic Engineering, Porosity, Deposition (law), Diatoms, biology, SERS, fungi, lcsh:Electronics, Diatom, Condensed Matter Physics, biology.organism_classification, Atomic and Molecular Physics, and Optics, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Chemical engineering, Colloidal gold, symbols, Surface modification, lcsh:T1-995, Raman spectroscopy, Biosensor, Bio-sensors
Abstract

Diatomaceous earth, or diatomite, is produced through the accumulation of diatom (Bacillariophyceae) skeletons (i.e. cell walls called frustules) made of amorphous silica. The porous, highly symmetrical structure and microscopic size of diatom cell walls make them ideal constituents of sensing devices and analytical chips. Here, we propose chemical methods to purify diatom frustules extracted from diatomaceous earth. Using photo deposition techniques, we grow gold nanoparticles on the surface of diatom skeletons and within the pores of the skeletons, where the size and density of nanoparticles can be controlled by changing the parameters of the synthesis. Resulting devices have an internal porous structure that can harvest molecules from a solution, and an external shell of gold nanoparticles that amplifies the electromagnetic field generated by the measurement laser in Raman or other spectroscopies. The combination of these effects enables the analysis of biological specimens, chemical analytes and pollutants in extremely low abundance ranges. The devices were demonstrated in the analysis of Bovine Serum Albumin in water with a concentration of 100 aM, and mineral oil with a concentration of 50 ppm. Keywords: Diatomite, Diatoms, Photo-deposition, Gold nanoparticles, SERS, Bio-sensors

Published
2019
Full Text
View/download PDF

5. Silica diatom shells tailored with Au nanoparticles enable sensitive analysis of molecules for biological, safety and environment applications

Author

Roksana Majewska, Nicola Coppedè, Francesco Gentile, Davide Calestani, Francesco Amato, Andrea Schirato, Marco Villani, Edmondo Battista, Maria Laura Coluccio, Valentina Onesto, E. Di Fabrizio, Mario Cesarelli, Alessandro Alabastri, Onesto, V., Villani, M., Coluccio, M. L., Majewska, R., Alabastri, A., Battista, E., Schirato, A., Calestani, D., Coppedé, N., Cesarelli, M., Amato, F., Di Fabrizio, E., and Gentile, F.

Subjects
Au functionalization, Materials science, Sensing device, Silicon dioxide, Nanochemistry, Nanoparticle, Nanotechnology, 02 engineering and technology, 01 natural sciences, Biological sensing, chemistry.chemical_compound, 0103 physical sciences, lcsh:TA401-492, medicine, Molecule, Gold Nanoparticles, General Materials Science, Mineral oil, 010302 applied physics, Diatoms, biology, Nano Express, SERS, fungi, Diatom, 021001 nanoscience & nanotechnology, Condensed Matter Physics, biology.organism_classification, Sensing devices, chemistry, Colloidal gold, Frustule, lcsh:Materials of engineering and construction. Mechanics of materials, Frustules, Materials Science (all), Safety, 0210 nano-technology, Drug carrier, medicine.drug
Abstract

Diatom shells are a natural, theoretically unlimited material composed of silicon dioxide, with regular patterns of pores penetrating through their surface. For their characteristics, diatom shells show promise to be used as low cost, highly efficient drug carriers, sensor devices or other micro-devices. Here, we demonstrate diatom shells functionalized with gold nanoparticles for the harvesting and detection of biological analytes (bovine serum albumin—BSA) and chemical pollutants (mineral oil) in low abundance ranges, for applications in bioengineering, medicine, safety, and pollution monitoring. Electronic supplementary material The online version of this article (10.1186/s11671-018-2507-4) contains supplementary material, which is available to authorized users.

Published
2018
Full Text
View/download PDF

6. Methylglyoxal Adducts Levels in Blood Measured on Dried Spot by Portable Near-Infrared Spectroscopy.

Author

Bonapace G, Gentile F, Coppedé N, Coluccio ML, Garo V, Vismara MFM, Candeloro P, Donato G, and Malara N

Abstract

The altered glucose metabolism characterising cancer cells determines an increased amount of methylglyoxal in their secretome. Previous studies have demonstrated that the methylglyoxal, in turn, modifies the protonation state (PS) of soluble proteins contained in the secretomes of cultivated circulating tumour cells (CTCs). In this study, we describe a method to assess the content of methylglyoxal adducts (MAs) in the secretome by near-infrared (NIR) portable handheld spectroscopy and the extreme learning machine (ELM) algorithm. By measuring the vibration absorption functional groups containing hydrogen, such as C-H, O-H and N-H, NIR generates specific spectra. These spectra reflect alterations of the energy frequency of a sample bringing information about its MAs concentration levels. The algorithm deciphers the information encoded in the spectra and yields a quantitative estimate of the concentration of MAs in the sample. This procedure was used for the comparative analysis of different biological fluids extracted from patients suspected of having cancer (secretome, plasma, serum, interstitial fluid and whole blood) measured directly on the solute left on a surface upon a sample-drop cast and evaporation, without any sample pretreatment. Qualitative and quantitative regression models were built and tested to characterise the different levels of MAs by ELM. The final model we selected was able to automatically segregate tumour from non-tumour patients. The method is simple, rapid and repeatable; moreover, it can be integrated in portable electronic devices for point-of-care and remote testing of patients.

Published
2021
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results