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3. The MinPET diamond discovery technique

Author

Nemakhavhani, T., Unwuchola, D., Andrew, R. C., Cook, M. N. H., Connell, S. H., Ballestrero, S. E., Ulrik Uggerhoj, Søren Pape Møller, Poul Aggerholm, Niels Hertel, Jacobus Andreas Swartz, and Engelbrecht, Japie more...

Published
2018

5. Acid-Sensing Ion Channel-1a in the Amygdala, a Novel Therapeutic Target in Depression-Related Behavior

Author

Coryell, M. W., primary, Wunsch, A. M., additional, Haenfler, J. M., additional, Allen, J. E., additional, Schnizler, M., additional, Ziemann, A. E., additional, Cook, M. N., additional, Dunning, J. P., additional, Price, M. P., additional, Rainier, J. D., additional, Liu, Z., additional, Light, A. R., additional, Langbehn, D. R., additional, and Wemmie, J. A., additional more...

Published
2009
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10. Mapping of quantitative trait loci with knockout/congenic strains.

Author

Bolivar, V J, Cook, M N, and Flaherty, L

Abstract

Recently we have explored the use of knockout/congenic mouse strains for isolating and mapping quantitative trait loci (QTLs). Because most knockout strains have been bred to be B6.129 congenic strains, they can be used to test for QTLs in the targeted chromosomal area as long as there is a genetic difference between B6 and 129. Thus, we have tested a number of knockout/congenic strains in a series of behavioral tests in which mouse performance has a significant genetic component. We have also developed a breeding scheme for distinguishing the effects of background flanking genes from the targeted ablation. In screening several knockout/congenics, we have found at least one that harbors a behavioral QTL in the 129 chromosomal segment. The position of this QTL was confirmed subsequently by several F1 crosses. more...

Published
2001
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11. Isolation and characterization of a cDNA clone encoding rat 5-lipoxygenase.

Author

Balcarek, J M, Theisen, T W, Cook, M N, Varrichio, A, Hwang, S M, Strohsacker, M W, and Crooke, S T

Abstract

A full-length cDNA clone encoding 5-lipoxygenase, a key enzyme in the formation of leukotrienes, was isolated from a rat basophilic leukemia cell lambda gt11 cDNA library. The 2.5-kilobase (kb) cDNA insert, whose identity was confirmed by hybrid-select translation and DNA sequence analysis, has a 2.0-kb open reading frame encoding a protein of Mr approximately 77,600 and includes 60 base pairs of 5′-untranslated region and 0.4 kb of 3′-untranslated region to the polyadenylation signal. The deduced amino acid sequence shows significant homology with published sequences for the rabbit reticulocyte lipoxygenase and soybean lipoxygenase-1; it also contains sequences similar to a consensus sequence found in several calcium-dependent membrane-binding proteins. The cDNA recognizes a 2.6-kb mRNA species which is detected in all tissues but is particularly abundant in RNA from lung. more...

Published
1988
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14. Differential effects of SB 242235, a selective p38 mitogen-activated protein kinase inhibitor, on IL-1 treated bovine and human cartilage/chondrocyte cultures.

Author

Badger AM, Roshak AK, Cook MN, Newman-Tarr TM, Swift BA, Carlson K, Connor JR, Lee JC, Gowen M, Lark MW, and Kumar S

Subjects
Animals, Cartilage, Articular drug effects, Cartilage, Articular metabolism, Cattle, Cell Culture Techniques, Chondrocytes metabolism, Culture Techniques, Dinoprostone antagonists & inhibitors, Gene Expression Regulation drug effects, Humans, Nitric Oxide biosynthesis, Nitric Oxide Synthase genetics, Proteoglycans biosynthesis, RNA, Messenger genetics, Species Specificity, p38 Mitogen-Activated Protein Kinases, Chondrocytes drug effects, Enzyme Inhibitors pharmacology, Imidazoles pharmacology, Interleukin-1 pharmacology, Mitogen-Activated Protein Kinases antagonists & inhibitors, Pyridines pharmacology
Abstract

The p38 MAP kinase inhibitor, SB 242235, was evaluated for its effects on the metabolism of bovine and human cartilage and primary chondrocyte cultures. SB 242235 had no effect on proteoglycan synthesis (PG) in bovine articular cartilage explants (BAC), as measured by [(35)S]-sulfate incorporation into glycosaminoglycans (GAGs). In addition, the compound had no effect on IL-1 alpha-induced GAG release from these cultures. However, there was a potent, dose-dependent inhibition of nitric oxide (NO) release from IL-1 alpha-stimulated BAC with an IC(50)of approximately 0.6 microM, with similar effects observed in primary chondrocytes. The effect on BAC was time dependent, and mechanistically did not appear to be the result of inhibition of protein kinase C (PKC), protein kinase A (PKA) or MEK-1. The effect on NO release in bovine chondrocytes was at the level of inducible nitric oxide synthase (iNOS) gene expression, which was inhibited at similar concentrations as nitrite production. In primary human chondrocytes, IL-1 beta induction of p38 MAP kinase was inhibited by SB 242235 with an IC(50)of approximately 1 microM. Surprisingly, however, treatment of IL-beta-stimulated human cartilage or chondrocytes with SB 242235 did not inhibit either NO production or the induction of iNOS. On the other hand, the natural product hymenialdisine (HYM), a protein tyrosine kinase (PTK) inhibitor, inhibited NO production and iNOS in both species. In contrast to the differential control of iNOS, PGE(2)was inhibited by SB 242235 in both IL-1-stimulated bovine and human chondrocyte cultures. These studies indicate that there are species differences in the control of iNOS by p38 inhibitors and also that different pathways may control IL-1-induced proteoglycan breakdown and NO production., (Copyright 2000 OsteoArthritis Research Society International.) more...

Published
2000
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