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5 results on '"Coipan, E."'

1. High Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Household Transmission Rates Detected by Dense Saliva Sampling

Author

MS Infectieziekten, Zorgeenheid Traumatologie, Infectieziekten onderzoek3 (Bogaert), Infection & Immunity, Immuno/reuma onderzoek 8 (Trzcinski), Sportgeneeskunde Onderwijs, Cluster B, Child Health, Immuno/reuma patientenzorg, Kolodziej, L M, van Lelyveld, S F L, Haverkort, M E, Mariman, R, Sluiter-Post, J G C, Badoux, P, de Koff, E M, Koole, J C D, Miellet, W R, Swart, A N, Coipan, E C, Meijer, A, Sanders, E A M, Trzciński, K, Euser, S M, Eggink, D, van Houten, M A, MS Infectieziekten, Zorgeenheid Traumatologie, Infectieziekten onderzoek3 (Bogaert), Infection & Immunity, Immuno/reuma onderzoek 8 (Trzcinski), Sportgeneeskunde Onderwijs, Cluster B, Child Health, Immuno/reuma patientenzorg, Kolodziej, L M, van Lelyveld, S F L, Haverkort, M E, Mariman, R, Sluiter-Post, J G C, Badoux, P, de Koff, E M, Koole, J C D, Miellet, W R, Swart, A N, Coipan, E C, Meijer, A, Sanders, E A M, Trzciński, K, Euser, S M, Eggink, D, and van Houten, M A

Published
2022

3. Circulation of four Anaplasma phagocytophilum ecotypes in Europe

Author

Jahfari, Setareh, Coipan, E Claudia, Fonville, Manoj, van Leeuwen, Arieke Docters, Hengeveld, Paul, Heylen, Dieter, Heyman, Paul, van Maanen, Cees, Butler, Catherine M, Földvári, Gábor, Szekeres, Sándor, van Duijvendijk, Gilian, Tack, Wesley, Rijks, Jolianne M, van der Giessen, Joke, Takken, Willem, van Wieren, Sipke E, Takumi, Katsuhisa, Sprong, Hein, Veterinair Pathologisch Diagnostisch Cnt, PB AVM, Advances in Veterinary Medicine, Veterinair Pathologisch Diagnostisch Cnt, PB AVM, and Advances in Veterinary Medicine

Subjects
Male, Veterinary medicine, Anaplasmosis, Epidemiology, animal diseases, Ixodes ricinus, human granulocytic anaplasmosis, Wildlife, Zoonoses, sequence-analysis, phylogenetic analyses, Laboratory of Entomology, Phylogeny, biology, PE&RC, candidatus neoehrlichia mikurensis, Europe, Infectious Diseases, Larva, Enzootic, Female, Ixodidae, Anaplasma phagocytophilum, Nymph, Ehrlichiosis, borrelia-burgdorferi, Human granulocytic anaplasmosis, ixodes-ricinus ticks, strains, parasitic diseases, medicine, Animals, Humans, Borrelia burgdorferi, gene, Biology, Research, borne diseases, Laboratorium voor Entomologie, biology.organism_classification, medicine.disease, bacterial infections and mycoses, Virology, Haplotypes, ehrlichiosis, Wildlife Ecology and Conservation, bacteria, Parasitology, Human medicine
Abstract

Background Anaplasma phagocytophilum is the etiological agent of granulocytic anaplasmosis in humans and animals. Wild animals and ticks play key roles in the enzootic cycles of the pathogen. Potential ecotypes of A. phagocytophilum have been characterized genetically, but their host range, zoonotic potential and transmission dynamics has only incompletely been resolved. Methods The presence of A. phagocytophilum DNA was determined in more than 6000 ixodid ticks collected from the vegetation and wildlife, in 289 tissue samples from wild and domestic animals, and 69 keds collected from deer, originating from various geographic locations in The Netherlands and Belgium. From the qPCR-positive lysates, a fragment of the groEL-gene was amplified and sequenced. Additional groEL sequences from ticks and animals from Europe were obtained from GenBank, and sequences from human cases were obtained through literature searches. Statistical analyses were performed to identify A. phagocytophilum ecotypes, to assess their host range and their zoonotic potential. The population dynamics of A. phagocytophilum ecotypes was investigated using population genetic analyses. Results DNA of A. phagocytophilum was present in all stages of questing and feeding Ixodes ricinus, feeding I. hexagonus, I. frontalis, I. trianguliceps, and deer keds, but was absent in questing I. arboricola and Dermacentor reticulatus. DNA of A. phagocytophilum was present in feeding ticks and tissues from many vertebrates, including roe deer, mouflon, red foxes, wild boar, sheep and hedgehogs but was rarely found in rodents and birds and was absent in badgers and lizards. Four geographically dispersed A. phagocytophilum ecotypes were identified, that had significantly different host ranges. All sequences from human cases belonged to only one of these ecotypes. Based on population genetic parameters, the potentially zoonotic ecotype showed significant expansion. Conclusion Four ecotypes of A. phagocytophilum with differential enzootic cycles were identified. So far, all human cases clustered in only one of these ecotypes. The zoonotic ecotype has the broadest range of wildlife hosts. The expansion of the zoonotic A. phagocytophilum ecotype indicates a recent increase of the acarological risk of exposure of humans and animals. Electronic supplementary material The online version of this article (doi:10.1186/1756-3305-7-365) contains supplementary material, which is available to authorized users.

Published
2014

4. Circulation of four Anaplasma phagocytophilum ecotypes in Europe

Author

Veterinair Pathologisch Diagnostisch Cnt, PB AVM, Advances in Veterinary Medicine, Jahfari, Setareh, Coipan, E Claudia, Fonville, Manoj, van Leeuwen, Arieke Docters, Hengeveld, Paul, Heylen, Dieter, Heyman, Paul, van Maanen, Cees, Butler, Catherine M, Földvári, Gábor, Szekeres, Sándor, van Duijvendijk, Gilian, Tack, Wesley, Rijks, Jolianne M, van der Giessen, Joke, Takken, Willem, van Wieren, Sipke E, Takumi, Katsuhisa, Sprong, Hein, Veterinair Pathologisch Diagnostisch Cnt, PB AVM, Advances in Veterinary Medicine, Jahfari, Setareh, Coipan, E Claudia, Fonville, Manoj, van Leeuwen, Arieke Docters, Hengeveld, Paul, Heylen, Dieter, Heyman, Paul, van Maanen, Cees, Butler, Catherine M, Földvári, Gábor, Szekeres, Sándor, van Duijvendijk, Gilian, Tack, Wesley, Rijks, Jolianne M, van der Giessen, Joke, Takken, Willem, van Wieren, Sipke E, Takumi, Katsuhisa, and Sprong, Hein

Published
2014

5. Downregulation of hsp22 gene expression in Drosophila melanogaster from sites located near chemical plants.

Author

Magdalena LM, Coipan EC, Vladimirescu AF, Savu L, Costache M, and Gavrila L

Subjects
Adaptation, Biological, Animals, Down-Regulation genetics, Stress, Physiological, Drosophila Proteins genetics, Drosophila melanogaster genetics, Environment, Gene Expression Regulation, Heat-Shock Proteins genetics
Abstract

A common physiological response of organisms to environmental conditions is variation in gene expression, especially true for genes encoding for heat shock proteins. In insects, this process has been examined for induced heat or cold stress. The putative long-term imprinted/acquired heat shock protein response due to unfriendly environmental conditions has been far less studied. The Drosophila melanogaster hsp22 gene, which has been extensively reviewed as being sensitive to different changing life conditions, was examined by qRT-PCR, using carboxy-X-rhodamine. In the present study, we focused on the detection of hsp22 level of transcription in three D. melanogaster isolates, collected from sites located near different chemical plants in Romania and subjected to one-year adaptation to laboratory conditions. In all isolates, the hsp22 gene expression was determined using the housekeeping genes Gapdh1 and UbcD10 as internal controls. According to our experimental results, the D. melanogaster hsp22 gene was significantly downregulated compared to the same gene in w(1118)iso, used as a calibrator. We showed that hsp22 could play an important role in relation to stress resistance and adaptation. This study highlights the importance of in vivo studies to demonstrate genome plasticity to overcome different damages induced by any presumed source of stress.

Published
2012
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