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Building long-lasting antimicrobial and clean surfaces is one of the most effective strategies to inhibit bacterial infection, but obtaining an ideal smart surface with highly efficient, controllable, and regenerative properties still encounters many challenges. Herein, we fabricate an ultrathin brush–hydrogel hybrid coating (PSBMA-P(HEAA-co-METAC)) by integrating antifouling polyzwitterionic (PSBMA) brushes and antimicrobial polycationic (P(HEAA-co-METAC)) hydrogels. The smart bacterial killing–releasing properties can be achieved independently by the opposite volume and conformation changes between the swelling (shrinking) of P(HEAA-co-METAC) hydrogel layer and the shrinking (swelling) of PSBMA brushes. The friction test reveals that both METAC and SBMA components support great lubrication. By tuning the initial organosilane (BrTMOS:KH570) ratios, the prepared PSBMA-P(HEAA-co-METAC) coating exhibits different antibacterial abilities from single “capturing–killing” to versatile “capturing–killing–releasing.” Most importantly, 99% of the bacterial-releasing rate can be easily achieved via 0.5 M NaCl treatment. This smart surface not only possesses long-lasting antibacterial performance, only ∼1.09 × 105 cell·cm−2 bacterial residue even after 72 h exposure to bacteria solutions, but also can be regenerated and triggered between water and salt solution multiple times. This work provides a new way to fabricate antibacterial smart hydrogel coatings with bacterial “killing–releasing” functions and shows great potential for biomedical applications.

Although diverse uranium (U) adsorbents have been explored, it is still a great challenge for high-efficient uranium extraction form seawater. Herein a wood-mimetic oriented porous Ti3C2Tx-MXene/gelatin hydrogel (MGH) has been explored through growing directional ice crystals cooled by liquid nitrogen and subsequently forming pores by freeze-dry (Ice-template) method, for ultrafast and high-efficient U-adsorption from seawater with great enhancement by both electric field and sunlight. Different from disperse Ti3C2Tx-MXene powder, this MGH not only can be easily utilized but also can own ultrahigh specific surface area for high-efficient U-adsorption. The U-adsorbing capacity of this MGH (10 mg) can reach 4.17 mg·g−1 after only 1 week in 100 kg of seawater, which is outstanding in existing adsorbents. Furthermore, on the positive pole of 0.4 V direct current source or under 1-sun irradiation, the U-adsorbing capacity of the MGH can increase by 57.11% and 13.57%, respectively. Most importantly, the U-adsorption of this hydrogel can be greatly enhanced by simultaneously using the above two methods, which can increase the U-adsorbing capacity by 79.95% reaching 7.51 mg·g−1. This work provides a new biomimetic porous MXene-based hydrogel for electric field/sunlight bi-enhanced high-efficient U-extraction from seawater, which will inspire new strategy to design novel U-adsorbents and systems.

Background: Previous studies have shown that alterations in the gut microbiota are closely associated with Acute Coronary Syndrome (ACS) development. However, the value of gut microbiota for early diagnosis of ACS remains understudied., Methods: We recruited 66 volunteers, including 29 patients with a first diagnosis of ACS and 37 healthy volunteers during the same period, collected their fecal samples, and sequenced the V4 region of the 16S rRNA gene. Functional prediction of the microbiota was performed using PICRUSt2. Subsequently, we constructed a nomogram and corresponding webpage based on microbial markers to assist in the diagnosis of ACS. The diagnostic performance and usefulness of the model were analyzed using boostrap internal validation, calibration curves, and decision curve analysis (DCA)., Results: Compared to that of healthy controls, the diversity and composition of microbial community of patients with ACS was markedly abnormal. Potentially pathogenic genera such as Streptococcus and Acinetobacter were significantly increased in the ACS group, whereas certain SCFA-producing genera such as Blautia and Agathobacter were depleted. In addition, in the correlation analysis with clinical indicators, the microbiota was observed to be associated with the level of inflammation and severity of coronary atherosclerosis. Finally, a diagnostic model for ACS based on gut microbiota and clinical variables was developed with an area under the receiver operating characteristic (ROC) curve (AUC) of 0.963 (95% CI: 0.925-1) and an AUC value of 0.948 (95% CI: 0.549-0.641) for bootstrap internal validation. The calibration curves of the model show good consistency between the actual and predicted probabilities. The DCA showed that the model had a high net clinical benefit for clinical applications., Conclusion: Our study is the first to characterize the composition and function of the gut microbiota in patients with ACS and healthy populations in Southwest China and demonstrates the potential effect of the microbiota as a non-invasive marker for the early diagnosis of ACS., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Wang, Hu, Xu, Shi, Cao, Ma, Wang, Zhang, Luo, Lin, Li, Duan and Cai.)

Objective: To evaluate the efficacy of HLA- haploidentical donor hematopoietic transplantation (Haplo- HSCT)for severe aplastic anemia (SAA)by compared with the same period of unrelated donor transplantation (UD- HSCT)., Methods: Of a cohort of 50 SAA patients between September 2012 and July 2014, 26 patients underwent UD- HSCT and 24 patients Haplo- HSCT., Results: OS rate was 91.3% with a median follow-up of 9 (2-26)months. According to transplant type, there was no significant difference between UD- and Haplo-HSCT (96.1%vs 86.0%,P=0.30). 3 of 50 (6%)patients had primary engraft failure. Haplo- HSCT developed higher significantly incidence of Ⅱ- Ⅳ aGVHD (37.5%vs 3.83%,P=0.003)and cGVHD (37.5%vs 15.3%,P=0.030)than UD-HSCT. Haplo-HSCT also had significantly higher incidences of CMV viremia (78.2%vs 46.1%,P=0.005)and EBV viremia (43.1%vs 16.0%,P=0.040), respectively than UD-HSCT. But the incidences of hemorrhagic cystitis were similar between two transplant types (39.1%vs 23.0%,P=0.120)., Conclusion: This study showed favorable outcome of Haplo-HSCT for SAA, which was comparable with UD-HSCT.