11 results on '"Bodmer, Jocelyn"'
Search Results
2. Postmortem Metabolism and Pork Quality Development Are Affected by Electrical Stimulation across Three Genetic Lines
- Author
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Spires, Matthew D., primary, Bodmer, Jocelyn S., additional, Beline, Mariane, additional, Wicks, Jordan C., additional, Zumbaugh, Morgan D., additional, Shi, Tim Hao, additional, Reichert, Brian T., additional, Schinckel, Allan P., additional, Grant, Alan L., additional, and Gerrard, David E., additional
- Published
- 2023
- Full Text
- View/download PDF
3. Avian mitochondria respiration is not predicated solely on complex abundance and DNA content
- Author
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Yen, Con-Ning, primary, Bodmer, Jocelyn, additional, Wicks, Jordan, additional, Shi, Tim, additional, and Gerrard, David, additional
- Published
- 2023
- Full Text
- View/download PDF
4. Postmortem Metabolism and Pork Quality Development Are Affected by Electrical Stimulation across Three Genetic Lines
- Author
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Spires, Matthew D., Bodmer, Jocelyn S., Beline, Mariane, Wicks, Jordan C., Zumbaugh, Morgan D., Shi, Tim Hao, Reichert, Brian T., Schinckel, Allan P., Grant, Alan L., Gerrard, David E., Spires, Matthew D., Bodmer, Jocelyn S., Beline, Mariane, Wicks, Jordan C., Zumbaugh, Morgan D., Shi, Tim Hao, Reichert, Brian T., Schinckel, Allan P., Grant, Alan L., and Gerrard, David E.
- Abstract
Variations in postmortem metabolism in muscle impact pork quality development. Curiously, some genetic lines are more refractile to adverse pork quality development than others and may regulate energy metabolism differently. The aim of this study was to challenge pork carcasses from different genetic populations with electrical stimulation (ES) to determine how postmortem metabolism varies with genetic line and explore control points that reside in glycolysis in dying muscle. Three genetic populations (GP) were subjected to ES (100 V or 200 V, 13 pulses, 2 s on/2 s off) at 15- or 25-min post-exsanguination, or no stimulation (NS). Genetic population affected relative muscle relative abundance of different myosin heavy chains, glycogen, G6P, and lactate concentrations. Genetic lines responded similarly to ES, but a comparison of ES treatment groups revealed a trend for an interaction between voltage, time of ES, and time postmortem. Higher voltage accelerated pH decline at 20 min up to 60 min postmortem. Trends in color and firmness scores and L* values were consistent with pH and metabolite data. These data show that genetic populations respond differently to postmortem perturbation by altering glycolytic flux and suggest differences in postmortem glycolysis may be partially responsible for differences in meat quality between genetic populations, though not entirely.
- Published
- 2023
5. Skeletal Muscle O-GlcNAc Transferase Action on Global Metabolism Is Partially Mediated Through Interleukin-15
- Author
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Zumbaugh, Morgan D., primary, Yen, Con-Ning, additional, Bodmer, Jocelyn S., additional, Shi, Hao, additional, and Gerrard, David E., additional
- Published
- 2021
- Full Text
- View/download PDF
6. Skeletal Muscle O-GlcNAc Transferase Action on Global Metabolism Is Partially Mediated Through Interleukin-15
- Author
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Zumbaugh, Morgan D., Yen, Con-Ning, Bodmer, Jocelyn S., Shi, Hao, Gerrard, David E., Zumbaugh, Morgan D., Yen, Con-Ning, Bodmer, Jocelyn S., Shi, Hao, and Gerrard, David E.
- Abstract
Besides its roles in locomotion and thermogenesis, skeletal muscle plays a significant role in global glucose metabolism and insulin sensitivity through complex nutrient sensing networks. Our previous work showed that the muscle-specific ablation of O-GlcNAc transferase (OGT) led to a lean phenotype through enhanced interleukin-15 (IL-15) expression. We also showed OGT epigenetically modified and repressed the Il15 promoter. However, whether there is a causal relationship between OGT ablation-induced IL-15 secretion and the lean phenotype remains unknown. To address this question, we generated muscle specific OGT and interleukin-15 receptor alpha subunit (IL-15r alpha) double knockout mice (mDKO). Deletion of IL-15r alpha in skeletal muscle impaired IL-15 secretion. When fed with a high-fat diet, mDKO mice were no longer protected against HFD-induced obesity compared to wild-type mice. After 22 weeks of HFD feeding, mDKO mice had an intermediate body weight and glucose sensitivity compared to wild-type and OGT knockout mice. Taken together, these data suggest that OGT action is partially mediated by muscle IL-15 production and provides some clarity into how disrupting the O-GlcNAc nutrient signaling pathway leads to a lean phenotype. Further, our work suggests that interfering with the OGT-IL15 nutrient sensing axis may provide a new avenue for combating obesity and metabolic disorders.
- Published
- 2021
- Full Text
- View/download PDF
7. Exploring the Factors Contributing to the High Ultimate pH of Broiler Pectoralis Major Muscles Affected by Wooden Breast Condition
- Author
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Baldi, Giulia, Yen, Con-Ning, Daughtry, Morgan R., Bodmer, Jocelyn, Bowker, Brian C., Zhuang, Hong, Petracci, Massimiliano, Gerrard, David E., Baldi, Giulia, Yen, Con-Ning, Daughtry, Morgan R., Bodmer, Jocelyn, Bowker, Brian C., Zhuang, Hong, Petracci, Massimiliano, and Gerrard, David E.
- Abstract
The elevated ultimate pH (pH(u)) found in wooden breast (WB) meat suggests an altered muscular energetic status in WB but also could be related to a prematurely terminated post-mortem pH decline. The aims of this study were to explore the factors contributing to the elevated pH(u) and establish whether the occurrence of WB defect alters muscle post-mortem carbohydrate metabolism and determine if the contractile apparatus reflects such changes. A total of 24 carcasses from Ross 308 male chickens were obtained from a commercial producer and harvested using commercial processing procedures. Carcasses were categorized into unaffected (NORM) and WB groups (n = 12 each), and samples were collected from cranial bone-in pectoralis major (PM) muscles at 15 min and 24 h post-mortem for the determination of pH, glycolytic metabolites, adenonucleotides, buffering capacity, phosphofructokinase (PFK) activity, and in vitro pH decline. Twenty-four additional deboned PM samples (12 NORM and 12 WB) were collected from the same processing plant to assess muscle histology and sarcomere length at four different locations throughout the PM muscle. Data show that the reduced glycolytic potential of WB muscles only partially explains the higher (P < 0.001) pH(u) of WB meat, as residual glycogen along with unaltered PFK activity suggests that neither glycogen nor a deficiency of PFK is responsible for arresting glycolysis prematurely. The dramatic reduction in ATP concentrations in the early post-mortem period suggests a defective ATP-generating pathway that might be responsible for the reduced pH decline in WB samples. Further, the addition of excess of ATPase extended post-mortem glycolysis of WB meat in an in vitro glycolytic system. WB-affected samples have longer (P < 0.001) sarcomeres compared to NORM, indicating the existence of compromised energy-generating pathways in myopathic muscles that may have had consequences on the muscle contraction and tension development, as in vivo, al
- Published
- 2020
- Full Text
- View/download PDF
8. Exploring the Factors Contributing to the High Ultimate pH of Broiler Pectoralis Major Muscles Affected by Wooden Breast Condition
- Author
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Animal and Poultry Sciences, Baldi, Giulia, Yen, Con-Ning, Daughtry, Morgan R., Bodmer, Jocelyn, Bowker, Brian C., Zhuang, Hong, Petracci, Massimiliano, Gerrard, David E., Animal and Poultry Sciences, Baldi, Giulia, Yen, Con-Ning, Daughtry, Morgan R., Bodmer, Jocelyn, Bowker, Brian C., Zhuang, Hong, Petracci, Massimiliano, and Gerrard, David E.
- Abstract
The elevated ultimate pH (pH(u)) found in wooden breast (WB) meat suggests an altered muscular energetic status in WB but also could be related to a prematurely terminated post-mortem pH decline. The aims of this study were to explore the factors contributing to the elevated pH(u) and establish whether the occurrence of WB defect alters muscle post-mortem carbohydrate metabolism and determine if the contractile apparatus reflects such changes. A total of 24 carcasses from Ross 308 male chickens were obtained from a commercial producer and harvested using commercial processing procedures. Carcasses were categorized into unaffected (NORM) and WB groups (n = 12 each), and samples were collected from cranial bone-in pectoralis major (PM) muscles at 15 min and 24 h post-mortem for the determination of pH, glycolytic metabolites, adenonucleotides, buffering capacity, phosphofructokinase (PFK) activity, and in vitro pH decline. Twenty-four additional deboned PM samples (12 NORM and 12 WB) were collected from the same processing plant to assess muscle histology and sarcomere length at four different locations throughout the PM muscle. Data show that the reduced glycolytic potential of WB muscles only partially explains the higher (P < 0.001) pH(u) of WB meat, as residual glycogen along with unaltered PFK activity suggests that neither glycogen nor a deficiency of PFK is responsible for arresting glycolysis prematurely. The dramatic reduction in ATP concentrations in the early post-mortem period suggests a defective ATP-generating pathway that might be responsible for the reduced pH decline in WB samples. Further, the addition of excess of ATPase extended post-mortem glycolysis of WB meat in an in vitro glycolytic system. WB-affected samples have longer (P < 0.001) sarcomeres compared to NORM, indicating the existence of compromised energy-generating pathways in myopathic muscles that may have had consequences on the muscle contraction and tension development, as in vivo, al
- Published
- 2020
9. Exploring the Factors Contributing to the High Ultimate pH of Broiler Pectoralis Major Muscles Affected by Wooden Breast Condition
- Author
-
Baldi, Giulia, primary, Yen, Con-Ning, additional, Daughtry, Morgan R., additional, Bodmer, Jocelyn, additional, Bowker, Brian C., additional, Zhuang, Hong, additional, Petracci, Massimiliano, additional, and Gerrard, David E., additional
- Published
- 2020
- Full Text
- View/download PDF
10. Exploring the Factors Contributing to the High Ultimate pH of Broiler Pectoralis Major Muscles Affected by Wooden Breast Condition
- Author
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Hong Zhuang, Con-Ning Yen, Massimiliano Petracci, Brian C. Bowker, David E. Gerrard, Giulia Baldi, Morgan R. Daughtry, Jocelyn Bodmer, Animal and Poultry Sciences, Baldi, Giulia, Yen, Con-Ning, Daughtry, Morgan R., Bodmer, Jocelyn, Bowker, Brian C., Zhuang, Hong, Petracci, Massimiliano, and Gerrard, David E.
- Subjects
medicine.medical_specialty ,Physiology ,ATPase ,Carbohydrate metabolism ,Biology ,Sarcomere ,lcsh:Physiology ,03 medical and health sciences ,chemistry.chemical_compound ,wooden breast, post-mortem metabolism, pH, sarcomere length, glycolysis ,Physiology (medical) ,Internal medicine ,medicine ,Glycolysis ,030304 developmental biology ,Original Research ,0303 health sciences ,sarcomere length ,lcsh:QP1-981 ,Glycogen ,pH ,post-mortem metabolism ,0402 animal and dairy science ,Broiler ,04 agricultural and veterinary sciences ,glycolysis ,040201 dairy & animal science ,wooden breast ,Endocrinology ,chemistry ,biology.protein ,medicine.symptom ,Muscle contraction ,Phosphofructokinase - Abstract
The elevated ultimate pH (pH(u)) found in wooden breast (WB) meat suggests an altered muscular energetic status in WB but also could be related to a prematurely terminated post-mortem pH decline. The aims of this study were to explore the factors contributing to the elevated pH(u) and establish whether the occurrence of WB defect alters muscle post-mortem carbohydrate metabolism and determine if the contractile apparatus reflects such changes. A total of 24 carcasses from Ross 308 male chickens were obtained from a commercial producer and harvested using commercial processing procedures. Carcasses were categorized into unaffected (NORM) and WB groups (n = 12 each), and samples were collected from cranial bone-in pectoralis major (PM) muscles at 15 min and 24 h post-mortem for the determination of pH, glycolytic metabolites, adenonucleotides, buffering capacity, phosphofructokinase (PFK) activity, and in vitro pH decline. Twenty-four additional deboned PM samples (12 NORM and 12 WB) were collected from the same processing plant to assess muscle histology and sarcomere length at four different locations throughout the PM muscle. Data show that the reduced glycolytic potential of WB muscles only partially explains the higher (P < 0.001) pH(u) of WB meat, as residual glycogen along with unaltered PFK activity suggests that neither glycogen nor a deficiency of PFK is responsible for arresting glycolysis prematurely. The dramatic reduction in ATP concentrations in the early post-mortem period suggests a defective ATP-generating pathway that might be responsible for the reduced pH decline in WB samples. Further, the addition of excess of ATPase extended post-mortem glycolysis of WB meat in an in vitro glycolytic system. WB-affected samples have longer (P < 0.001) sarcomeres compared to NORM, indicating the existence of compromised energy-generating pathways in myopathic muscles that may have had consequences on the muscle contraction and tension development, as in vivo, also during the post-mortem period. Considering the overall reduced glycolytic potential and the myodegenerative processes associated with WB condition, we speculate that the higher pH(u) of WB meat might be the outcome of a drastically impaired energy-generating pathway combined with a deficiency and/or a dysfunction of muscle ATPases, having consequences also on muscle fiber contraction degree.
- Published
- 2020
11. Determining muscle plasticity and meat quality development of low-input extended fed market-ready steers.
- Author
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Wicks JC, Wivell AL, Beline M, Zumbaugh MD, Bodmer JS, Yen CN, Johnson-Schuster C, Wilson TB, Greiner SP, Johnson SE, Shi TH, Silva SL, and Gerrard DE
- Abstract
In March 2020, the World Health Organization declared COVID-19 a pandemic, which ultimately led to many meat processors temporarily shutting down or reducing processing capacity. This backlog in processing capacity forced many feedlots to retain cattle for longer periods of time and assume the risk of major market fluctuations. The aim of this study was to understand how a dietary insult affects meat quality and muscle metabolism in market-ready steers (590 kg). Sixteen market-ready (590 kg) commercial Angus crossbred steers were subjected to a maintenance diet of either forage or grain for 60 d. Longissimus lumborum (LL) muscle samples were collected immediately postmortem and processed for characteristics reflecting the underlying muscle fiber type and energy state of the tissue. Despite cattle being subjected to a 60-d feeding period, there were no detectable differences ( P > 0.05) in carcass characteristics, color of lean, or ultimate pH (pH
u ). Moreover, our data show that muscle plasticity is rather resilient, as reflected by lack of significance ( P > 0.05) in oxidative and glycolytic enzymes, myosin heavy chain isoforms (MyHC), myoglobin, and mitochondrial DNA (mtDNA) contents. These data show that market-ready steers are capable of withstanding a low-input feeding strategy up to 60 d without dramatically impacting underlying muscle characteristics and meat quality development., Competing Interests: There is no conflict of interest for all authors that could be perceived as prejudicing the impartiality of the research reported., (© The Author(s) 2024. Published by Oxford University Press on behalf of the American Society of Animal Science.)- Published
- 2024
- Full Text
- View/download PDF
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