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2. Preparation of natural three‑dimensional goat kidney scaffold for the development of bioartificial organ.

Author

Vishwakarma, S. K., Bhavani, P. G., Bardia, A., Abkari, A., Murthy, G. S. N., Venkateshwarulu, J., and Khan, A. A.

Subjects
*TISSUE engineering, *ACADEMIC medical centers, *ANGIOGRAPHY, *ANIMAL experimentation, *ARTIFICIAL organs, *KIDNEYS, *POLYMERASE chain reaction
Abstract

The high prevalence of end‑stage kidney diseases demands new treatment strategies. Decellularization approach may provide a viable option grow organs using a regenerative medicine approach. Goat kidney was decellularized by perfusion decellularization using detergents to produce an cellular construct for kidney scaffold. After pre‑treatment with anticoagulant, the decellularized scaffold was analyzed for its intact three‑dimensional natural architecture and vasculature. Perfusion of decellularized kidney preserved the structure and composition of renal extra‑cellular matrix and vascular structures within the scaffold. No evidence of residual cellular components was found. This approach provides a model for understanding of whole organ regeneration. [ABSTRACT FROM AUTHOR]

Published
2014
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