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3. Ecological and metabolic implications of the nurse effect of Maihueniopsis camachoi in the Atacama Desert

Author

Díaz, Francisca P., primary, Dussarrat, Thomas, additional, Carrasco‐Puga, Gabriela, additional, Colombié, Sophie, additional, Prigent, Sylvain, additional, Decros, Guillaume, additional, Bernillon, Stéphane, additional, Cassan, Cédric, additional, Flandin, Amélie, additional, Guerrero, Pablo C., additional, Gibon, Yves, additional, Rolin, Dominique, additional, Cavieres, Lohengrin A., additional, Pétriacq, Pierre, additional, Latorre, Claudio, additional, and Gutiérrez, Rodrigo A., additional

Published
2023
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4. Ecological and metabolic implications of the nurse effect of Maihueniopsis camachoi in the Atacama Desert.

Author

Díaz, Francisca P., Dussarrat, Thomas, Carrasco‐Puga, Gabriela, Colombié, Sophie, Prigent, Sylvain, Decros, Guillaume, Bernillon, Stéphane, Cassan, Cédric, Flandin, Amélie, Guerrero, Pablo C., Gibon, Yves, Rolin, Dominique, Cavieres, Lohengrin A., Pétriacq, Pierre, Latorre, Claudio, and Gutiérrez, Rodrigo A.

Subjects
DESERTS, PHYTOGEOGRAPHY, TEMPERATURE distribution, BIOMARKERS, MACHINE learning
Abstract

Summary: Plant–plant positive interactions are key drivers of community structure. Yet, the underlying molecular mechanisms of facilitation processes remain unexplored. We investigated the 'nursing' effect of Maihueniopsis camachoi, a cactus that thrives in the Atacama Desert between c. 2800 and 3800 m above sea level. We hypothesised that an important protective factor is thermal amelioration of less cold‐tolerant species with a corresponding impact on molecular phenotypes.To test this hypothesis, we compared plant cover and temperatures within the cactus foliage with open areas and modelled the effect of temperatures on plant distribution. We combined eco‐metabolomics and machine learning to test the molecular consequences of this association.Multiple species benefited from the interaction with M. camachoi. A conspicuous example was the extended distribution of Atriplex imbricata to colder elevations in association with M. camachoi (400 m higher as compared to plants in open areas). Metabolomics identified 93 biochemical markers predicting the interaction status of A. imbricata with 79% accuracy, independently of year.These findings place M. camachoi as a key species in Atacama plant communities, driving local biodiversity with an impact on molecular phenotypes of nursed species. Our results support the stress‐gradient hypothesis and provide pioneer insights into the metabolic consequences of facilitation. [ABSTRACT FROM AUTHOR]

Published
2024
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5. Leaf metabolomic data of eight sunflower lines and their sixteen hybrids under water deficit☆

Author

Berton Thierry, Bernillon Stéphane, Fernandez Olivier, Duruflé Harold, Flandin Amélie, Cassan Cédric, Jacob Daniel, Langlade Nicolas B., Gibon Yves, and Moing Annick

Subjects
helianthus, abiotic stress, drought stress, lc-ms, metabolomic profiling, Oils, fats, and waxes, TP670-699
Abstract

This article describes how metabolomic data were produced on sunflower plants subjected to water deficit. Twenty-four sunflower (Helianthus annuus L.) genotypes were selected to represent genetic diversity within cultivated sunflower and included both inbred lines and their hybrids. Drought stress was applied at the vegetative stage to plants cultivated in pots using the high-throughput phenotyping facility Heliaphen. Here, we provide untargeted and targeted metabolomic data of sunflower leaves. These compositional data differentiate both plant water status and different genotype groups. They constitute a valuable resource for the community to study the adaptation of crops to drought and the metabolic bases of heterosis.

Published
2021
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9. Predictive metabolomics of multiple Atacama plant species unveils a core set of generic metabolites for extreme climate resilience

Author

Dussarrat, Thomas, primary, Prigent, Sylvain, additional, Latorre, Claudio, additional, Bernillon, Stéphane, additional, Flandin, Amélie, additional, Díaz, Francisca P., additional, Cassan, Cédric, additional, Van Delft, Pierre, additional, Jacob, Daniel, additional, Varala, Kranthi, additional, Joubes, Jérôme, additional, Gibon, Yves, additional, Rolin, Dominique, additional, Gutiérrez, Rodrigo A., additional, and Pétriacq, Pierre, additional

Published
2022
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10. Extensive metabolic cross-talk in melon fruit revealed by spatial and developmental combinatorial metabolomics

Author

Moing, Annick, Aharoni, Asaph, Biais, Benoit, Rogachev, Ilana, Meir, Sagit, Brodsky, Leonid, Allwood, J. William, Erban, Alexander, Dunn, Warwick B., Kay, Lorraine, de Koning, Sjaak, de Vos, Ric C. H., Jonker, Harry, Mumm, Roland, Deborde, Catherine, Maucourt, Michael, Bernillon, Stéphane, Gibon, Yves, Hansen, Thomas H., Husted, Søren, Goodacre, Royston, Kopka, Joachim, Schjoerring, Jan K., Rolin, Dominique, and Hall, Robert D.

Published
2011

12. Plant Nitrate Reductases Regulate Nitric Oxide Production and Nitrogen-Fixing Metabolism During the Medicago truncatula–Sinorhizobium meliloti Symbiosis

Author

BERGER, Antoine, BOSCARI, Alexandre, HORTA ARAÚJO, Natasha, MAUCOURT, Mickaël, HANCHI, Mohamed, BERNILLON, Stéphane, ROLIN, Dominique, PUPPO, Alain, BROUQUISSE, Renaud, Institut Sophia Agrobiotech (ISA), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Côte d'Azur (UCA), University of Florida [Gainesville] (UF), Biologie du fruit et pathologie (BFP), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Platetorme Métabolome Bordeaux, Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche en Agronomie, Centre National de la Recherche Scientifique (CNRS), Côte d'Azur University, ANR-11-INBS-0010,METABOHUB,Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation(2011), and ANR-15-CE20-0005,STAYPINK,Mécanismes contrôlant la transition entre fixation d'azote et sénescence dans les nodosités symbiotiques de légumineuses(2015)

Subjects
nitrogen-fixing symbiosis, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, nitrate reductase, Métabolisme, hypoxia, nitric oxide, legumes, [SDV]Life Sciences [q-bio], Medicago truncatula, food and beverages, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, nodules
Abstract

Nitrate reductase (NR) is the first enzyme of the nitrogen reduction pathway in plants, leading to the production of ammonia. However, in the nitrogen-fixing symbiosis between legumes and rhizobia, atmospheric nitrogen (N 2) is directly reduced to ammonia by the bacterial nitrogenase, which questions the role of NR in symbiosis. Next to that, NR is the best-characterized source of nitric oxide (NO) in plants, and NO is known to be produced during the symbiosis. In the present study, we first surveyed the three NR genes (MtNR1, MtNR2, and MtNR3) present in the Medicago truncatula genome and addressed their expression, activity, and potential involvement in NO production during the symbiosis between M. truncatula and Sinorhizobium meliloti. Our results show that MtNR1 and MtNR2 gene expression and activity are correlated with NO production throughout the symbiotic process and that MtNR1 is particularly involved in NO production in mature nodules. Moreover, NRs are involved together with the mitochondrial electron transfer chain in NO production throughout the symbiotic process and energy regeneration in N 2-fixing nodules. Using an in vivo NMR spectrometric approach, we show that, in mature nodules, NRs participate also in the regulation of energy state, cytosolic pH, carbon and nitrogen metabolism under both normoxia and hypoxia. These data point to the importance of NR activity for the N 2-fixing symbiosis and provide a first explanation of its role in this process.

Published
2020
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13. The Tomato Guanylate-Binding Protein SlGBP1 Enables Fruit Tissue Differentiation by Maintaining Endopolyploid Cells in a Non-Proliferative State

Author

Musseau, Constance, primary, Jorly, Joana, additional, Gadin, Stéphanie, additional, Sørensen, Iben, additional, Deborde, Catherine, additional, Bernillon, Stéphane, additional, Mauxion, Jean-Philippe, additional, Atienza, Isabelle, additional, Moing, Annick, additional, Lemaire-Chamley, Martine, additional, Rose, Jocelyn K.C., additional, Chevalier, Christian, additional, Rothan, Christophe, additional, Fernandez-Lochu, Lucie, additional, and Gévaudant, Frédéric, additional

Published
2020
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14. Using metabolomic data to predict Maize yield

Author

Prigent, Sylvain, Fernandez, Olivier, Bernillon, Stéphane, Pétriacq, Pierre, Moing, Annick, Berton, Thierry, Cabrera-Bosquet, Llorenç, Millet, Émilie, Welcker, Claude, Tardieu, François, Gibon, Yves, Biologie du fruit et pathologie (BFP), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Plateforme Metabolome MetaboHUB INRA Nouvelle Aquitaine, Institut National de la Recherche Agronomique (INRA), Écophysiologie des Plantes sous Stress environnementaux (LEPSE), Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Gauthier, Muriel

Subjects
[SDV] Life Sciences [q-bio], Métabolome, Plante céréalière, Métabolisme, [SDV]Life Sciences [q-bio], Métabolite, Maïs, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, rendement, ComputingMilieux_MISCELLANEOUS, Métabolomique
Abstract

International audience

Published
2019

15. Front. plant sci

Author

ROCH, Léa, Dai, Zhanwu, GOMES, Eric, Bernillon, Stéphane, Wang, Jiaojiao, Gibon, Yves, Moing, Annick, Biologie du fruit et pathologie (BFP), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), and Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
Organic Acids, Cross-Species, food and beverages, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Inter-Species, Metabolism Regulation, Qualité du fruit, Amino Acids, Primary Metabolism, Plante Fruitière, Sugars, Fleshy Fruit, Développement du fruit
Abstract

UMR BFP - Equipe Métabolisme; Although fleshy fruit species are economically important worldwide and crucial for human nutrition, the regulation of their fruit metabolism remains to be described finely. Fruit species differ in the origin of the tissue constituting the flesh, duration

Published
2019
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16. Mycotoxin biosynthesis and central metabolism are two interlinked pathways in Fusarium graminearum, as demonstrated by the extensive metabolic changes induced by caffeic acid exposure

Author

Chéreau, Sylvain, Atanasova-Penichon, Vessela, Legoahec, Laurie, Bernillon, Stéphane, Deborde, Catherine, Maucourt, Mickael, Verdal-Bonnin, Marie-Noëlle, Pinson-Gadais, Laetitia, Moing, Annick, Ponts, Nadia, Richard-Forget, Florence, Unité de recherche Mycologie et Sécurité des Aliments (MycSA), Institut National de la Recherche Agronomique (INRA), Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, and Universität für Bodenkultur Wien [Vienne, Autriche] (BOKU). AUT.

Subjects
[SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

17. Regulation of Pyridine Nucleotide Metabolism During Tomato Fruit Development Through Transcript and Protein Profiling

Author

Decros, Guillaume, primary, Beauvoit, Bertrand, additional, Colombié, Sophie, additional, Cabasson, Cécile, additional, Bernillon, Stéphane, additional, Arrivault, Stéphanie, additional, Guenther, Manuela, additional, Belouah, Isma, additional, Prigent, Sylvain, additional, Baldet, Pierre, additional, Gibon, Yves, additional, and Pétriacq, Pierre, additional

Published
2019
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19. MeRy-B: a web knowledgebase for the storage, visualization, analysis and annotation of plant NMR metabolomic profiles

Author

Bernillon Stéphane, Moing Annick, Deborde Catherine, Gil Laurent, Ferry-Dumazet Hélène, Rolin Dominique, Nikolski Macha, de Daruvar Antoine, and Jacob Daniel

Subjects
Botany, QK1-989
Abstract

Abstract Background Improvements in the techniques for metabolomics analyses and growing interest in metabolomic approaches are resulting in the generation of increasing numbers of metabolomic profiles. Platforms are required for profile management, as a function of experimental design, and for metabolite identification, to facilitate the mining of the corresponding data. Various databases have been created, including organism-specific knowledgebases and analytical technique-specific spectral databases. However, there is currently no platform meeting the requirements for both profile management and metabolite identification for nuclear magnetic resonance (NMR) experiments. Description MeRy-B, the first platform for plant 1H-NMR metabolomic profiles, is designed (i) to provide a knowledgebase of curated plant profiles and metabolites obtained by NMR, together with the corresponding experimental and analytical metadata, (ii) for queries and visualization of the data, (iii) to discriminate between profiles with spectrum visualization tools and statistical analysis, (iv) to facilitate compound identification. It contains lists of plant metabolites and unknown compounds, with information about experimental conditions, the factors studied and metabolite concentrations for several plant species, compiled from more than one thousand annotated NMR profiles for various organs or tissues. Conclusion MeRy-B manages all the data generated by NMR-based plant metabolomics experiments, from description of the biological source to identification of the metabolites and determinations of their concentrations. It is the first database allowing the display and overlay of NMR metabolomic profiles selected through queries on data or metadata. MeRy-B is available from http://www.cbib.u-bordeaux2.fr/MERYB/index.php.

Published
2011
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20. Increase of Fungal Pathogenicity and Role of Plant Glutamine in Nitrogen-Induced Susceptibility (NIS) To Rice Blast

Author

Huang, Huichuan, Nguyen Thi Thu, Thuy, He, Xiahong, Gravot, Antoine, Bernillon, Stéphane, Ballini, Elsa, Morel, Jean-Benoit, State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Faculty of Agronomy, Hue University of Agriculture and Forestry (HUAF), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Natural Science Foundation of Yunnan Province of China : 2016FD025, Vietnamese government, Chinese government, ANR-SYSTERRA program, MetaboHUB program : ANR-11-INBS-0010, Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), ANR-11-INBS-0010,METABOHUB,Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation(2011), Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA)-Université Bordeaux Segalen - Bordeaux 2, and Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)

Subjects
Vegetal Biology, rice, food and beverages, Plant Science, Magnaporthe oryzae, fertilizer, nitrogen, defense, glutamine, notrogen, pathogenicity, effector, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, protection des cultures, Biologie végétale, Original Research
Abstract

BFP Equipe MétabolismeBGPI : équipe 4; International audience; Modifications in glutamine synthetase OsGS1-2 expression and fungal pathogenicity underlie nitrogen-induced susceptibility to rice blast.Understanding why nitrogen fertilization increase the impact of many plant diseases is of major importance. The interaction between Magnaporthe oryzae and rice was used as a model for analyzing the molecular mechanisms underlying Nitrogen-Induced Susceptibility (NIS). We show that our experimental system in which nitrogen supply strongly affects rice blast susceptibility only slightly affects plant growth. In order to get insights into the mechanisms of NIS, we conducted a dual RNA-seq experiment on rice infected tissues under two nitrogen fertilization regimes. On the one hand, we show that enhanced susceptibility was visible despite an over-induction of defense gene expression by infection under high nitrogen regime. On the other hand, the fungus expressed to high levels effectors and pathogenicity-related genes in plants under high nitrogen regime. We propose that in plants supplied with elevated nitrogen fertilization, the observed enhanced induction of plant defense is over-passed by an increase in the expression of the fungal pathogenicity program, thus leading to enhanced susceptibility. Moreover, some rice genes implicated in nitrogen recycling were highly induced during NIS. We further demonstrate that the OsGS1-2 glutamine synthetase gene enhances plant resistance to M. oryzae and abolishes NIS and pinpoint glutamine as a potential key nutrient during NIS.

Published
2017
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21. Tomato Guanylate-Binding Protein SlGBP1 Enables Fruit Tissue Differentiation by Maintaining Endopolyploid Cells in a Non-Proliferative State.

Author

Musseau, Constance, Jorly, Joana, Gadin, Stéphanie, Sørensen, Iben, Deborde, Catherine, Bernillon, Stéphane, Mauxion, Jean-Philippe, Atienza, Isabelle, Moing, Annick, Lemaire-Chamley, Martine, Rose, Jocelyn K.C., Chevalier, Christian, Rothan, Christophe, Fernandez-Lochu, Lucie, and Gévaudant, Frédéric

Published
2020
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22. Mycotoxin Biosynthesis and Central Metabolism Are Two Interlinked Pathways in Fusarium graminearum, as Demonstrated by the Extensive Metabolic Changes Induced by Caffeic Acid Exposure

Author

Atanasova-Penichon, Vessela, primary, Legoahec, Laurie, additional, Bernillon, Stéphane, additional, Deborde, Catherine, additional, Maucourt, Mickaël, additional, Verdal-Bonnin, Marie-Noëlle, additional, Pinson-Gadais, Laetitia, additional, Ponts, Nadia, additional, Moing, Annick, additional, and Richard-Forget, Florence, additional

Published
2018
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23. Integrative and predictive biology outside the plant: linking computational tools to fleshy fruit systems

Author

Cabasson, Cecile, Colombie, Sophie, Beauvoit, Bertrand, Nazaret, Christine, Benard, Camille, Dieuaide Noubhani, Martine, Rolin, Dominique, Bernillon, Stéphane, Deborde, Catherine, Jacob, Daniel, Moing, Annick, Maucourt, Mickael, Belouah, Isma, Roch, Léa, Dartigues, Benjamin, Nikolski, Macha, Mazat, Jean-Pierre, Gibon, Yves, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS), and ProdInra, Migration

Subjects
Metadata, Fruit development, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, Primary metabolism, Metabolic flux modelling, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2016

24. A chemical genetic strategy identify the PHOSTIN, a synthetic molecule that triggers phosphate starvation responses in Arabidopsis thaliana

Author

BONNOT, Clémence, PINSON, Benoît, CLÉMENT, Mathilde, BERNILLON, Stéphane, CHIARENZA, Serge, KANNO, Satomi, KOBAYASHI, Natsuko I., DELANNOY, Etienne, NAKANISHI, Tomoko M, NUSSAUME, Laurent, DESNOS, Thierry, Plant Environmental Physiology and Stress Signaling (PEPSS), Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut de biochimie et génétique cellulaires (IBGC), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB), Tokyo University of Science [Tokyo], Graduate School of Agricultural and Life Sciences [UTokyo] (GSALS), The University of Tokyo (UTokyo), ANR-09-BLAN-0118,CHEMIGENA,Génétique d'une voie limitant la croissance racinaire d'Arabidopsis en réponse à la carence phosphatée(2009), ANR-08-KBBE-0006,FOSSI,Phosphate signalling(2008), Signalisation de l'Adaptation des Végétaux à l'Environnement (SAVE), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Graduate School of Agricultural and Life Sciences, Department of Biomaterial Sciences, and The University of Tokyo

Subjects
phosphate starvation, Full Paper, Arabidopsis thaliana, Arabidopsis Proteins, Research, [SDV]Life Sciences [q-bio], Arabidopsis, Oryza sativa, Isoxazoles, Full Papers, Plants, Genetically Modified, Plant Roots, Phosphates, Small Molecule Libraries, chemical genetics, phosphate homeostasis, Gene Expression Regulation, Plant, PHOSTIN, Ubiquitin-Conjugating Enzymes, Homeostasis, phr1, phl1 mutant, ComputingMilieux_MISCELLANEOUS, Signal Transduction, Transcription Factors
Abstract

International audience; Plants display numerous strategies to cope with phosphate (Pi)-deficiency. Despite multiple genetic studies, the molecular mechanisms of low-Pi-signalling remain unknown. To validate the interest of chemical genetics to investigate this pathway we discovered and analysed the effects of PHOSTIN (PSN), a drug mimicking Pi-starvation in Arabidopsis. We assessed the effects of PSN and structural analogues on the induction of Pi-deficiency responses in mutants and wild-type and followed their accumulation in plants organs by high pressure liquid chromotography (HPLC) or mass-spectrophotometry. We show that PSN is cleaved in the growth medium, releasing its active motif (PSN11), which accumulates in plants roots. Despite the overaccumulation of Pi in the roots of treated plants, PSN11 elicits both local and systemic Pi-starvation effects. Nevertheless, albeit that the transcriptional activation of low-Pi genes by PSN11 is lost in the phr1;phl1 double mutant, neither PHO1 nor PHO2 are required for PSN11 effects. The range of local and systemic responses to Pi-starvation elicited, and their dependence on the PHR1/PHL1 function suggests that PSN11 affects an important and early step of Pi-starvation signalling. Its independence from PHO1 and PHO2 suggest the existence of unknown pathway(s), showing the usefulness of PSN and chemical genetics to bring new elements to this field.

Published
2016
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25. Leaf marker metabolites for low nitrogen stress in maize

Author

Urrutia Rosauro, Maria, Bernillon, Stéphane, Maucourt, M., Deborde, Catherine, Jacob, Daniel, Ballias, Patricia, Sellier, H., Gibon, Yves, Quillere, Isabelle, Hirel, Bertrand, Giauffret, Catherine, Moing, Annick, ProdInra, Archive Ouverte, Unité d'Agronomie de Laon-Reims-Mons (AGRO-LRM), Institut National de la Recherche Agronomique (INRA), Inconnu, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA)-Université Bordeaux Segalen - Bordeaux 2, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Agroressources et Impacts environnementaux (AgroImpact), and Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], Leaf marker metabolites, nitrogen stress, maize
Abstract

CT2 ; Département BAP; Leaf marker metabolites for low nitrogen stress in maize. Plant Biology Europe EPSO/FESPB 2016

Published
2016

26. Flux balance modelling in developing tomato fruit: the respiration climacteric as an emergent property

Author

Colombie, Sophie, Beauvoit, Bertrand, Nazaret, Christine, Bénard, Camille, Cabasson, Cécile, Maucourt, Mickael, Bernillon, Stéphane, Moing, Annick, Dieuaide Noubhani, Martine, Mazat, Jean-Pierre, Gibon, Yves, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Université de Bordeaux (UB), Centre National de la Recherche Scientifique (CNRS), Réseau Francophone de Métabolomique et Fluxomique (RFMF). FRA., and ProdInra, Migration

Subjects
Based model, Constraint, Flux balance analysis, Respiration climateric, Central metabolism, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, Carbon allocation, Environmental stress, Metabolic flux modelling, Tomato fruit development, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2016

27. Using metabolomic approaches and modelling of central metabolic to study fleshy fruit quality

Author

Colombie, Sophie, Beauvoit, Bertrand, Rolin, Dominique, Bernillon, Stéphane, Bénard, Camille, Prodhomme, Duyên, Maucourt, Mickael, Andrieu, Marie-Hélène, Ballias, Patricia, Deborde, Catherine, Cabasson, Cécile, Moing, Annick, Gallusci, Philippe, Teyssier, Emeline, Stammitti, Linda, Bertrand, Anne, Hooks, Mark, Dieuaide Noubhani, Martine, Gibon, Yves, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2015

28. La métabolomique : un outil de la génomique fonctionnelle au service de la biologie des systèmes

Author

Rolin, Dominique, Beauvoit, Bertrand, Bernillon, Stéphane, Bénard, Camille, Ballias, Patricia, Deborde, Catherine, Cabasson, Cécile, Colombie, Sophie, Dieuaide Noubhani, Martine, Gallusci, Philippe, Jacob, Daniel, Moing, Annick, Mazat, Jean-Pierre, Prodhomme, Duyên, Teyssier, Emeline, Zhendre, Vanessa, Gibon, Yves, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Société Française de Biologie Végétale (SFBV). FRA., and ProdInra, Migration

Subjects
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2014

29. Metabolomics of growth and type B trichothecenes production in Fusarium graminearum

Author

Legoahec, Laurie, Atanasova-Penichon, Vessela, Ponts, Nadia, Deborde, Catherine, Maucourt, Mickael, Bernillon, Stéphane, Moing, Annick, Richard-Forget, Florence, Unité de recherche Mycologie et Sécurité des Aliments (MycSA), Institut National de la Recherche Agronomique (INRA), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, ProdInra, Migration, Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), and Unité de recherche Mycologie et Sécurité des Aliments (MSA)

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, Fusarium graminearum, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, secondary metabolite, metabolomics, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2014

30. Bioactive stilbenes from Vitis vinifera grapevine shoots extracts

Author

CHAHER, Nassima, Arraki, Kamel, Dillinseger, Elsa, Temsamani, Hamza, Bernillon, Stéphane, Pedrot, Eric, Delaunay, Jean-Claude, Merillon, Jean-Michel, Monti, Jean-Pierre, Izard, Jean-Claude, Atmani, Djebbar, Richard, Tristan, Université Abderrahmane Mira [Béjaïa], Université Sciences et Technologies - Bordeaux 1, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Actichem S.A., and Partenaires INRAE

Subjects
Spectrometry, Mass, Electrospray Ionization, Industrial Waste, amyloid-β, Protein Aggregation, Pathological, Protein Aggregates, Phenols, Drug Discovery, Stilbenes, Humans, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Vitis, Chromatography, High Pressure Liquid, Nootropic Agents, Benzofurans, Amyloid beta-Peptides, Molecular Structure, Plant Extracts, Agriculture, Stereoisomerism, Peptide Fragments, NMR, LC-MS, stilbene, Neuroprotective Agents, Vitis vinifera, France, Stilbestrols, Plant Shoots
Abstract

Viticultural residues from commercial viticultural activities represent a potentially important source of bioactive stilbenes such as resveratrol. The main aim of the present study was therefore to isolate, identify and perform biological assays against amyloid-β peptide aggregation of original stilbenes from Vitis vinifera shoots.A new resveratrol oligomer, (Z)-cis-miyabenol C (3), was isolated from Vitis vinifera grapevine shoots together with two newly reported oligostilbenes from Vitis vinifera shoots, vitisinol C (1) and (E)-cis-miyabenol C (2), and six known compounds: piceatannol, resveratrol, (E)-ε-viniferin (trans-ε-viniferin), ω-viniferin, vitisinol C and (E)-miyabenol C. The structures of these resveratrol derivatives were established on the basis of detailed spectroscopic analysis including nuclear magnetic resonance experiments. All the newly reported compounds were tested for their anti-aggregative activity against amyloid-β fibril formation. Vitisinol C was found to exert a significant activity against amyloid-β aggregation.Vitis vinifera grapevine shoots are potentially interesting as a source of new bioactive stilbenes, such as vitisinol C.

Published
2014
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31. New cyclolignans from Origanum glandulosum active against beta-amyloid aggregation

Author

Basli, Abdelkader, Delaunay, Jean-Claude, Pedrot, Eric, Bernillon, Stéphane, Madani, Khodir, Monti, Jean-Pierre, Merillon, Jean-Michel, Chibane, Mohamed, Richard, Tristan, Université de Bordeaux Ségalen [Bordeaux 2], Université Abderrahmane Mira [Béjaïa], Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Université AMO Bouira, Partenaires INRAE, and PROFAS program

Subjects
composition phénolique, afrique du nord, Origanum glandulosum, oregano, cyclolignans, beta-Amyloid aggregation, [SDV]Life Sciences [q-bio], acide rosmarinique, origanum, qualité aromatique, lignane, flore endémique, herbe, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, huile essentielle
Abstract

International audience; Origanum glandulosum Desf is an endemic flavoring herb widely distributed in North Africa that is commonly used in traditional medicine. This oregano species is rich in essential oils but little is known about its phenolic composition. In the present study, a crude extract of O. glandulosum was prepared in order to isolate and investigate its neuroprotective potential to inhibit beta-amyloid peptide (A beta) aggregation. The three major compounds of the extract were isolated: rosmarinic acid and two cyclolignans in Origanum genus, globoidnan A and a new derivative named globoidnan B. Rosmarinic acid and globoidnan A showed significant anti-aggregative activity against beta amyloid aggregation (IC50 7.0 and 12.0 mu M, respectively). In contrast, globoidnan B was found to be less active.

Published
2014

32. Respiration climacteric in tomato fruits elucidated by constraint‐based modelling

Author

Colombié, Sophie, primary, Beauvoit, Bertrand, additional, Nazaret, Christine, additional, Bénard, Camille, additional, Vercambre, Gilles, additional, Le Gall, Sophie, additional, Biais, Benoit, additional, Cabasson, Cécile, additional, Maucourt, Mickaël, additional, Bernillon, Stéphane, additional, Moing, Annick, additional, Dieuaide‐Noubhani, Martine, additional, Mazat, Jean‐Pierre, additional, and Gibon, Yves, additional

Published
2016
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33. Combining systems biology, genome resequencing and MAGIC population to decipher the genetic variation of tomato fruit quality

Author

PASCUAL, Laura, XU, Jiaxin, DESPLAT, Nelly, GIBON, Yves, MOING, Annick, MAUCOURT, Mickael, BERNILLON, Stéphane, DEBORDE, Catherine, BOUCHET, Jean-Paul, BRUNEL, Dominique, LE PASLIER, Marie-Christine, CAUSSE, Mathilde, Génétique et Amélioration des Fruits et Légumes (GAFL), Institut National de la Recherche Agronomique (INRA), College of horticulture, Northwest Agriculture and Forestry University, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), and Etude du Polymorphisme des Génomes Végétaux (EPGV)

Subjects
polymorphisme nucléotidique simple (SNP), [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, tomate, enzyme, diversité génétique, lycopersicon esculentum, cellule de péricarpe, protéome, Sciences agricoles, qualité du fruit, ComputingMilieux_MISCELLANEOUS, Agricultural sciences, analyse du métabolisme
Abstract

International audience

Published
2013

34. Metabolomic and molecular profiling of a slow ripening peach selection reveals new mechanisms controlling mesocarp development

Author

Rasori, A., Maucourt, Mickael, Bernillon, Stéphane, Deborde, Catherine, Moing, Annick, Ramina, A., Bonghi, C., Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), Universita degli Studi di Padova, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), International Society for Horticultural Science (ISHS). INT., and ProdInra, Migration

Subjects
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2013

35. Diurnal compositional changes of tomato fruit and leaf

Author

BENARD, Camille, Bernillon, Stéphane, Osorio-Algar, Sonia, Maucourt, Mickael, Biais, Benoit, Labadie Lemiere, Emilie, Ballias, Patricia, Deborde, Catherine, Cabasson, Cécile, Jacob, Daniel, Génard, Michel, Fernie, Alisdair, Rolin, Dominique, Gautier, Hélène, Gibon, Yves, Moing, Annick, ProdInra, Migration, Unité de recherche Plantes et Systèmes de Culture Horticoles (PSH), Institut National de la Recherche Agronomique (INRA), Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Max Planck Institute of Molecular Plant Physiology (MPI-MP), and Max-Planck-Gesellschaft

Subjects
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2013

36. Présentation de la Plateforme Métabolome de Bordeaux

Author

Fouillen, Laetitia, Bernillon, Stéphane, ProdInra, Migration, Laboratoire de biogenèse membranaire (LBM), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Biologie du fruit et pathologie (BFP), and Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2013

37. Metabolomics data integration in tomato plant-fruit systems biology

Author

Bénard, Camille, Rolin, Dominique, Bernillon, Stéphane, Maucourt, Mickael, Biais, Benoit, Osorio-Algar, Sonia, Labadie Lemiere, Emilie, Ballias, Patricia, Deborde, Catherine, Cabasson, Cécile, Moing, Annick, Génard, Michel, Fernie, A., Fell, D., Poolman, M., Gautier, Hélène, Gibon, Yves, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Max Planck Institute of Molecular Plant Physiology (MPI-MP), Max-Planck-Gesellschaft, Unité de recherche Plantes et Systèmes de Culture Horticoles (PSH), Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2012

39. Tools and applications in plant metabolomics at Bordeaux Metabolome Fluxome Facility (PMFB; http://www.bordeaux.inra.fr/umr619/NMR.htm)

Author

Deborde, Catherine, Bernillon, Stéphane, Maucourt, Mickael, Cabasson, Cécile, Biais, Benoit, Ballias, Patricia, Prodhomme, Duyên, Menard, Guillaume, Jacob, Daniel, Masciocchi, Joël, Gibon, Yves, Rolin, Dominique, Moing, Annick, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Centre de Bioinformatique de Bordeaux (CBIB), CGFB, and Labo/service de l'auteur, Ville service.

Subjects
physiologie végétale, Vegetal Biology, métabolome, plateforme expérimentale, fluxomique, bordeaux, fluxome, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Biologie végétale, pôle metabolome, métabolomique, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2011

40. Développement d'outils informatiques pour le traitement et l'analyse des données en RMN-1H, dans un contexte d'expériences métabolomiques

Author

Jacob, Daniel, Deborde, Catherine, Bernillon, Stéphane, Moing, Annick, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Centre de Bioinformatique de Bordeaux (CBIB), CGFB, and Labo/service de l'auteur, Ville service.

Subjects
analyse de données, imaginerie rmn 1h, Vegetal Biology, métabolome, base de données en ligne, physiologie végétale, outil informatique, métabolite, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, bioinformatique, Biologie végétale, métabolomique, traitement de données, profil métabolique, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2011

41. Variation journalière de la composition métabolique de fruit de tomate en cours de maturation

Author

BENARD, Camille, Bernillon, Stéphane, Maucourt, Mickael, Biais, Benoit, Osorio-Algar, Sonia, Labadie Lemiere, Emilie, Ballias, Patricia, Deborde, Catherine, Cabasson, Cécile, Génard, Michel, Fernie, Alisdair, Rolin, Dominique, Gautier, Hélène, Gibon, Yves, Unité de recherche Plantes et Systèmes de Culture Horticoles (PSH), Institut National de la Recherche Agronomique (INRA), Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Max Planck Institute of Molecular Plant Physiology (MPI-MP), Max-Planck-Gesellschaft, Station de physiologie végétale, and Labo/service de l'auteur, Ville service.

Subjects
métabolisme carbone, Vegetal Biology, métabolome, fruit, variation diurne, physiologie végétale, solanum lycopersicum, métabolite, maturation du fruit, développement du fruit, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, tomate, fruit, variation journalière, métabolome, composition métabolique, ComputingMilieux_MISCELLANEOUS, Biologie végétale, métabolomique
Abstract

National audience

Published
2011

42. A chemical genetic strategy identify thePHOSTIN, a synthetic molecule that triggers phosphate starvation responses inArabidopsis thaliana

Author

Bonnot, Clémence, primary, Pinson, Benoît, additional, Clément, Mathilde, additional, Bernillon, Stéphane, additional, Chiarenza, Serge, additional, Kanno, Satomi, additional, Kobayashi, Natsuko, additional, Delannoy, Etienne, additional, Nakanishi, Tomoko M., additional, Nussaume, Laurent, additional, and Desnos, Thierry, additional

Published
2015
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43. Chemotypic variation explaining traditional selection of tropical root crops in Vanuatu, South Pacific

Author

Champagne, Antoine, Bernillon, Stéphane, Hilbert, Ghislaine, Legendre, Laurent, and Lebot, Vincent

Subjects
F60 - Physiologie et biochimie végétales, food and beverages, Q04 - Composition des produits alimentaires, F30 - Génétique et amélioration des plantes
Abstract

Understanding chemical variations in edible plant organs is essential for a better understanding of crop genetic diversity in traditional fields as well as for selection. Among tropical root crop species, high variation has been shown in the composition and content of isoprenoids and flavonoids. The purpose of this work was to identify relations between local preferences, primary compound and secondary metabolite contents, to attempt characterizing a good cultivar according to traditional customs and knowledge. A core-collection of about 500 cultivars was built to represent the widest agro-morphological variability, and analyses were made for percentage of dry matter, starch, minerals, cellulose, proteins and total sugars. In terms of primary compounds, very high coefficients of variation were found for protein, total sugar, cellulose and mineral contents, while starch exhibited the lowest variation. Starch content was always positively correlated with dry matter content, whereas it was negatively correlated with protein, mineral, total sugar and cellulose contents significant at 0.01% level. Through blind tests on organoleptic properties, local consumers were shown to prefer consuming their national dish with high starch and dry matter content, giving a particular cultivar high value in the eyes of local consumers. In contrast, preferences for daily consumption of boiled or roasted tubers were linked to average starch content, indicating great opportunities for improvement of others primary compound contents. Interestingly, isoprenoids content were strongly correlated to flavonol and flavanol contents among yam species, while phenolic acids content exhibited positive correlation with anthocyanins. This work suggests biofortification opportunities in isoprenoids and flavonoids content by colour-based selection. The database we established will help breeders improve micronutrient contents and health benefits of such staple foods. (Texte intégral)

Published
2009

44. Profils métaboliques de plantes tropicales à racines et tubercules du Vanouatou (Mélanésie)

Author

Champagne, Antoine, Bernillon, Stéphane, Moing, Annick, Legendre, L., Rolin, Dominique, Lebot, V., Université Jean Monnet [Saint-Étienne] (UJM), Station de physiologie végétale, Institut National de la Recherche Agronomique (INRA), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), and ProdInra, Migration

Subjects
BIOLOGIE VEGETALE, ISOPRENOIDE, F60 - Physiologie et biochimie végétale, [SDV]Life Sciences [q-bio], CHIMIOTYPE, RACINE, Plante racine, F30 - Génétique et amélioration des plantes, [SDV] Life Sciences [q-bio], PROFIL METABOLIQUE, PLANTE TROPICALE, TUBERCULE, HPLC, ComputingMilieux_MISCELLANEOUS
Abstract

Etant donné l'importance des plantes à racines et tubercules dans de nombreux pays tropicaux, il serait grandement utile pour l'amélioration génétique des cultures de rente ou vivrières, de disposer de données précises sur les compositions chimiques des génotypes qui seront sélectionnés sur leurs valeurs propres. Ce travail porte essentiellement sur des plantes, qui sont d'un intérêt majeur pour les pays des régions tropicales: le taro, Colocasia esculenta; le macabo, Xanthosoma sagittifolium; l'alocase, Alocasia macrorrhyza; des ignames, Dioscorea alata, D. bulbifera, D. esculenta, D. cayenensis, D. pentaphylla; la patate douce, Ipomoea batatas; le manioc, Manhiot esculenta. Selon les variétés, les organes de stockage présentent des couleurs très variées, allant du blanc au violet foncé en passant par le jaune, l'orange, le rose, le rouge et le violet. Ces organes peuvent être de couleur uniforme, bicolore, voire tricolore, parfois, avec des fibres colorées ou non. Ces couleurs sont associées à des métabolites secondaires, des pigments, présents en concentrations variables, notamment des isoprénoïdes responsables de couleurs allant du jaune au rouge. C'est le subtil mélange de ces molécules qui donne les diverses couleurs de chair observées. Un échantillon représentant les principales plantes à racines et tubercules consommées en Mélanésie, et au Vanouatou en particulier, a été choisi pour constituer un large spectre de plantes appartenant à quatre familles botaniques différentes. Dans une optique de sélection de ces plantes tropicales, le travail porte donc sur l'étude de la variabilité inter-spécifique des profils métaboliques. L'amélioration de plantes à multiplication végétative passe, en effet, par le choix judicieux de parents ainsi que par le criblage de grands nombres au niveau des descendances. 163 échantillons lyophilisés ont été extraits et les profils réalisés par CLHP, puis les teneurs et compositions ont été déterminées à l'aide d'un détecteur à barrette de diodes. Les standards CLHP, choisis dans la voie de biosynthèse des caroténoïdes, permettent d'établir des profils métaboliques, et d'étudier la variabilité au niveau interspécifique.

Published
2008

45. Respiration climacteric in tomato fruits elucidated by constraint-based modelling.

Author

Colombié, Sophie, Beauvoit, Bertrand, Nazaret, Christine, Bénard, Camille, Vercambre, Gilles, Le Gall, Sophie, Biais, Benoit, Cabasson, Cécile, Maucourt, Mickaël, Bernillon, Stéphane, Moing, Annick, Dieuaide‐Noubhani, Martine, Mazat, Jean‐Pierre, and Gibon, Yves

Subjects
TOMATOES, PLANT metabolism, PLANT physiology, RESPIRATION in plants, GAS exchange in plants, PHYSIOLOGY
Abstract

Tomato is a model organism to study the development of fleshy fruit including ripening initiation. Unfortunately, few studies deal with the brief phase of accelerated ripening associated with the respiration climacteric because of practical problems involved in measuring fruit respiration., Because constraint-based modelling allows predicting accurate metabolic fluxes, we investigated the respiration and energy dissipation of fruit pericarp at the breaker stage using a detailed stoichiometric model of the respiratory pathway, including alternative oxidase and uncoupling proteins. Assuming steady-state, a metabolic dataset was transformed into constraints to solve the model on a daily basis throughout tomato fruit development., We detected a peak of CO2 released and an excess of energy dissipated at 40 d post anthesis ( DPA) just before the onset of ripening coinciding with the respiration climacteric. We demonstrated the unbalanced carbon allocation with the sharp slowdown of accumulation (for syntheses and storage) and the beginning of the degradation of starch and cell wall polysaccharides. Experiments with fruits harvested from plants cultivated under stress conditions confirmed the concept., We conclude that modelling with an accurate metabolic dataset is an efficient tool to bypass the difficulty of measuring fruit respiration and to elucidate the underlying mechanisms of ripening. [ABSTRACT FROM AUTHOR]

Published
2017
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46. Increase of Fungal Pathogenicity and Role of Plant Glutamine in Nitrogen-Induced Susceptibility (NIS) To Rice Blast.

Author

Huichuan Huang, Thuy Nguyen Thi Thu, Xiahong He, Gravot, Antoine, Bernillon, Stéphane, Ballini, Elsa, and Morel, Jean-Benoit

Subjects
RICE blast disease, NITROGEN fertilizers, RNA sequencing
Abstract

Highlight Modifications in glutamine synthetase OsGS1-2 expression and fungal pathogenicity underlie nitrogen-induced susceptibility to rice blast. Understanding why nitrogen fertilization increase the impact of many plant diseases is of major importance. The interaction between Magnaporthe oryzae and rice was used as a model for analyzing the molecular mechanisms underlying Nitrogen-Induced Susceptibility (NIS). We show that our experimental system in which nitrogen supply strongly affects rice blast susceptibility only slightly affects plant growth. In order to get insights into the mechanisms of NIS, we conducted a dual RNA-seq experiment on rice infected tissues under two nitrogen fertilization regimes. On the one hand, we show that enhanced susceptibility was visible despite an over-induction of defense gene expression by infection under high nitrogen regime. On the other hand, the fungus expressed to high levels effectors and pathogenicity-related genes in plants under high nitrogen regime. We propose that in plants supplied with elevated nitrogen fertilization, the observed enhanced induction of plant defense is over-passed by an increase in the expression of the fungal pathogenicity program, thus leading to enhanced susceptibility. Moreover, some rice genes implicated in nitrogen recycling were highly induced during NIS. We further demonstrate that the OsGS1-2 glutamine synthetase gene enhances plant resistance to M. oryzae and abolishes NIS and pinpoint glutamine as a potential key nutrient during NIS. [ABSTRACT FROM AUTHOR]

Published
2017
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47. Bioguided Isolation, Characterization, and Biotransformation by Fusarium verticillioides of Maize Kernel Compounds That Inhibit Fumonisin Production

Author

Atanasova-Penichon, Vessela, primary, Bernillon, Stéphane, additional, Marchegay, Gisèle, additional, Lornac, Aurélia, additional, Pinson-Gadais, Laetitia, additional, Ponts, Nadia, additional, Zehraoui, Enric, additional, Barreau, Christian, additional, and Richard-Forget, Florence, additional

Published
2014
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48. Metabolomic and elemental profiling of melon fruit quality as affected by genotype and environment

Author

Bernillon, Stéphane, Biais, Benoit, Deborde, Catherine, Maucourt, Mickaël, Cabasson, Cécile, Gibon, Yves, Hansen, Thomas Hesselhøj, Husted, Søren, de Vos, Ric C.H., Mumm, Roland, Jonker, Harry, Ward, Jane L., Miller, Sonia J., Baker, John M., Burger, Joseph, Tadmor, Ya'akov, Beale, Michael H., Schjørring, Jan Kofod, Schaffer, Arthur A., Rolin, Dominique, Hall, Robert D., Moing, Annick, Bernillon, Stéphane, Biais, Benoit, Deborde, Catherine, Maucourt, Mickaël, Cabasson, Cécile, Gibon, Yves, Hansen, Thomas Hesselhøj, Husted, Søren, de Vos, Ric C.H., Mumm, Roland, Jonker, Harry, Ward, Jane L., Miller, Sonia J., Baker, John M., Burger, Joseph, Tadmor, Ya'akov, Beale, Michael H., Schjørring, Jan Kofod, Schaffer, Arthur A., Rolin, Dominique, Hall, Robert D., and Moing, Annick

Abstract

Melon (Cucumis melo L.) is a global crop in terms of economic importance and nutritional quality. The aim of this study was to explore the variability in metabolite and elemental composition of several commercial varieties of melon in various environmental conditions. Volatile and non-volatile metabolites as well as mineral elements were profiled in the flesh of mature fruit, employing a range of complementary analytical technologies. More than 1,000 metabolite signatures and 19 mineral elements were determined. Data analyses revealed variations related to factors such as variety, growing season, contrasting agricultural management practices (greenhouse vs. field with or without fruit thinning) and planting date. Two hundred and ninety-one analytes discriminated two contrasting varieties, one from the var. inodorous group and the other from the var. cantaloupensis group. Two hundred and eighty analytes discriminated a short shelf-life from a mid-shelf-life variety within the var. cantaloupensis group. Three hundred and twenty-seven analytes discriminated two seasons, and two hundred and fifty-two analytes discriminated two contrasting agricultural management practices. The affected compound families greatly depended on the factor studied. The compositional variability of identified or partially identified compounds was used to study metabolite and mineral element co-regulation using correlation networks. The results confirm that metabolome and mineral element profiling are useful diagnostic tools to characterize the quality of fruits cultivated under commercial conditions. They can also provide knowledge on fruit metabolism and the mechanisms of plant response to environmental modifications, thereby paving the way for metabolomics-guided improvement of cultural practices for better fruit quality.

Published
2013

49. (Homo)glutathione deficiency impairs root-knot nematode development in Medicago truncatula.

Author

Baldacci-Cresp, Fabien, Chang, Christine, Maucourt, Mickaël, Deborde, Catherine, Hopkins, Julie, Lecomte, Philippe, Bernillon, Stéphane, Brouquisse, Renaud, Moing, Annick, Abad, Pierre, Hérouart, Didier, Puppo, Alain, Favery, Bruno, Frendo, Pierre, Baldacci-Cresp, Fabien, Chang, Christine, Maucourt, Mickaël, Deborde, Catherine, Hopkins, Julie, Lecomte, Philippe, Bernillon, Stéphane, Brouquisse, Renaud, Moing, Annick, Abad, Pierre, Hérouart, Didier, Puppo, Alain, Favery, Bruno, and Frendo, Pierre

Abstract

Root-knot nematodes (RKN) are obligatory plant parasitic worms that establish and maintain an intimate relationship with their host plants. During a compatible interaction, RKN induce the redifferentiation of root cells into multinucleate and hypertrophied giant cells essential for nematode growth and reproduction. These metabolically active feeding cells constitute the exclusive source of nutrients for the nematode. Detailed analysis of glutathione (GSH) and homoglutathione (hGSH) metabolism demonstrated the importance of these compounds for the success of nematode infection in Medicago truncatula. We reported quantification of GSH and hGSH and gene expression analysis showing that (h)GSH metabolism in neoformed gall organs differs from that in uninfected roots. Depletion of (h)GSH content impaired nematode egg mass formation and modified the sex ratio. In addition, gene expression and metabolomic analyses showed a substantial modification of starch and γ-aminobutyrate metabolism and of malate and glucose content in (h)GSH-depleted galls. Interestingly, these modifications did not occur in (h)GSH-depleted roots. These various results suggest that (h)GSH have a key role in the regulation of giant cell metabolism. The discovery of these specific plant regulatory elements could lead to the development of new pest management strategies against nematodes., info:eu-repo/semantics/published

Published
2012

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