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8. TRANSPLANTATION BASIC SCIENCE, ALLOGENIC AND XENOGENIC TOLERANCE

Author

Berthelot, L., primary, Robert, T., additional, Tabary, T., additional, Vuiblet, V., additional, Drame, M., additional, Toupance, O., additional, Rieu, P., additional, Monteiro, R. C., additional, Toure, F., additional, Ferrario, S., additional, Cantaluppi, V., additional, De Lena, M., additional, Dellepiane, S., additional, Beltramo, S., additional, Rossetti, M., additional, Manzione, A. M., additional, Messina, M., additional, Gai, M., additional, Dolla, C., additional, Biancone, L., additional, Camussi, G., additional, Pontrelli, P., additional, Oranger, A. R., additional, Accetturo, M., additional, Rascio, F., additional, Gigante, M., additional, Castellano, G., additional, Schena, A., additional, Fiorentino, M., additional, Zito, A., additional, Zaza, G., additional, Stallone, G., additional, Gesualdo, L., additional, Grandaliano, G., additional, Pattonieri, E. F., additional, Gregorini, M., additional, Corradetti, V., additional, Rocca, C., additional, Milanesi, S., additional, Peloso, A., additional, Ferrario, J., additional, Cannone, M., additional, Bosio, F., additional, Maggi, N., additional, Avanzini, M. A., additional, Minutillo, P., additional, Paulli, M., additional, Maestri, M., additional, Rampino, T., additional, Dal Canton, A., additional, Wu, K. S. T., additional, Coxall, O., additional, Luque, Y., additional, Candon, S., additional, Rabant, M., additional, Noel, L.-H., additional, Thervet, E., additional, Chatenoud, L., additional, Snanoudj, R., additional, Anglicheau, D., additional, Legendre, C., additional, Zuber, J., additional, Hruba, P., additional, Brabcova, I., additional, Krepsova, E., additional, Slatinska, J., additional, Sekerkova, A., additional, Striz, I., additional, Zachoval, R., additional, Viklicky, O., additional, Scholbach, T. M., additional, Wang, H.-K., additional, Loong, C.-C., additional, Yang, A.-H., additional, Wu, T.-H., additional, Guberina, H., additional, Rebmann, V., additional, Dziallas, P., additional, Dolff, S., additional, Wohlschlaeger, J., additional, Heinemann, F. M., additional, Witzke, O., additional, Zoet, Y. M., additional, Claas, F. H. J., additional, Horn, P. A., additional, Kribben, A., additional, Doxiadis, I. I. N., additional, Prasad, N., additional, Yadav, B., additional, Agarwal, V., additional, Jaiswal, A., additional, Rai, M., additional, Hope, C. M., additional, Coates, P. T., additional, Heeger, P. S., additional, Carroll, R., additional, Masola, V., additional, Secchi, M. F., additional, Onisto, M., additional, Gambaro, G., additional, Lupo, A., additional, Matsuyama, M., additional, Kobayashi, T., additional, Yoneda, Y., additional, Chargui, J., additional, Touraine, J. L., additional, Yoshimura, R., additional, Vizza, D., additional, Perri, A., additional, Lupinacci, S., additional, Toteda, G., additional, Lofaro, D., additional, Leone, F., additional, Gigliotti, P., additional, La Russa, A., additional, Papalia, T., additional, Bonofilgio, R., additional, Sentis Fuster, A., additional, Kers, J., additional, Yapici, U., additional, Claessen, N., additional, Bemelman, F. J., additional, Ten Berge, I. J. M., additional, Florquin, S., additional, Glotz, D., additional, Rostaing, L., additional, Squifflet, J.-P., additional, Merville, P., additional, Belmokhtar, C., additional, Le Ny, G., additional, Lebranchu, Y., additional, Papazova, D. A., additional, Friederich-Persson, M., additional, Koeners, M. P., additional, Joles, J. A., additional, Verhaar, M. C., additional, Trivedi, H. L., additional, Vanikar, A. V., additional, Dave, S. D., additional, Suarez Alvarez, B., additional, Garcia Melendreras, S., additional, Carvajal Palao, R., additional, Diaz Corte, C., additional, Ruiz Ortega, M., additional, Lopez-Larrea, C., additional, Yadav, A. K., additional, Bansal, D., additional, Kumar, V., additional, Minz, M., additional, Jha, V., additional, Kaminska, D., additional, Koscielska-Kasprzak, K., additional, Chudoba, P., additional, Mazanowska, O., additional, Banasik, M., additional, Zabinska, M., additional, Boratynska, M., additional, Lepiesza, A., additional, Korta, K., additional, Klinger, M., additional, Csohany, R., additional, Prokai, A., additional, Pap, D., additional, Balicza-Himer, N., additional, Vannay, A., additional, Fekete, A., additional, Kis-Petik, K., additional, Peti-Peterdi, J., additional, Szabo, A., additional, Masajtis-Zagajewska, A., additional, Muras, K., additional, Niewodniczy, M., additional, Nowicki, M., additional, Pascual, J., additional, Srinivas, T. R., additional, Chadban, S., additional, Citterio, F., additional, Henry, M., additional, Oppenheimer, F., additional, Lee, P.-C., additional, Tedesco-Silva, H., additional, Zeier, M., additional, Watarai, Y., additional, Dong, G., additional, Hexham, M., additional, Bernhardt, P., additional, Vincenti, F., additional, Rocchetti, M. T., additional, Su owicz, J., additional, Wojas-Pelc, A., additional, Ignacak, E., additional, Janda, K., additional, Krzanowski, M., additional, Su owicz, W., additional, Mitsuhashi, M., additional, Murakami, T., additional, Benso, A., additional, Leuning, D., additional, Reinders, M., additional, Lievers, E., additional, Duijs, J., additional, Van Zonneveld, A. J., additional, Van Kooten, C., additional, Engelse, M., additional, Rabelink, T., additional, Assounga, A., additional, Omarjee, S., additional, Ngema, Z., additional, Ersoy, A., additional, Gultepe, A., additional, Isiktas Sayilar, E., additional, Akalin, H., additional, Coskun, F., additional, Oner Torlak, M., additional, Ayar, Y., additional, Riegersperger, M., additional, Plischke, M., additional, Steinhauser, C., additional, Jallitsch-Halper, A., additional, Sengoelge, G., additional, Winkelmayer, W. C., additional, Sunder-Plassmann, G., additional, Foedinger, M., additional, Kaziuk, M., additional, Kuz'Niewski, M., additional, B Tkowska- Prokop, A., additional, Pa Ka, K., additional, Dumnicka, P., additional, Kolber, W., additional, and Su Owicz, W., additional

Published
2014
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10. Diabetes - experimental models

Author

Blanco-Gozalo, V., primary, Blazquez-Medela, A., additional, Garcia-Sanchez, O., additional, Quiros, Y., additional, Montero, M., additional, Martinez-Salgado, C., additional, Lopez-Hernandez, F., additional, Lopez-Novoa, J., additional, Yao, L., additional, Qing, Z., additional, Hua, X., additional, Min, F., additional, Fei, M., additional, Ning, W., additional, Cantaluppi, V., additional, Figliolini, F., additional, Delena, M., additional, Beltramo, S., additional, Medica, D., additional, Tetta, C., additional, Segoloni, G., additional, Biancone, L., additional, Camussi, G., additional, Cunha, J. S., additional, Ferreira, V. M., additional, Naves, M. A., additional, Boim, M. A., additional, Zitman-Gal, T., additional, Golan, E., additional, Green, J., additional, Pasmanik-Chor, M., additional, Bernheim, J., additional, Benchetrit, S., additional, Riera, M., additional, Clotet, S., additional, Pascual, J., additional, Soler, M., additional, Nakai, K., additional, Fujii, H., additional, Kono, K., additional, Goto, S., additional, Hirata, M., additional, Shinohara, M., additional, Fukagawa, M., additional, Nishi, S., additional, Fan, Q., additional, Du, S., additional, Jiang, Y., additional, Wang, L., additional, Fang, L., additional, Radovits, T., additional, Mozes, M. M., additional, Rosivall, L., additional, Kokeny, G., additional, Aoki, R., additional, Tateoka, R., additional, Sekine, F., additional, Kikuchi, K., additional, Yamashita, Y., additional, Itoh, Y., additional, Cappuccino, L., additional, Garibotto, G., additional, D'Amato, E., additional, Villaggio, B., additional, Gianiorio, F., additional, Mij, M., additional, Viazzi, F., additional, Salvidio, G., additional, Verzola, D., additional, Piwkowska, A., additional, Rogacka, D., additional, Audzeyenka, I., additional, Kasztan, M., additional, Angielski, S., additional, Jankowski, M., additional, Gaber, E. W., additional, El-Attar, H. A., additional, Liu, J., additional, Zhang, W., additional, He, Y., additional, Macsai, E., additional, Takats, Z., additional, Derzbach, L., additional, Korner, A., additional, Vasarhelyi, B., additional, Huang, M. S., additional, Bo, H., additional, Liu, F., additional, Fu, P., additional, Tsotakos, N. E., additional, Tsilibary, E. C., additional, Drossopoulou, G. I., additional, Thawho, N., additional, Farid, N., additional, Peleg, A., additional, Levy, A., additional, Nakhoul, N., additional, Lenghel, A. R., additional, Borza, G., additional, Catoi, C., additional, Bondor, C. I., additional, Muresan, A., additional, Kacso, I. M., additional, Song, J.-S., additional, Song, J.-H., additional, Ahn, S.-H., additional, Choi, B. S., additional, Hong, Y. a., additional, Kim, M. Y., additional, Lim, J. H., additional, Yang, K.-S., additional, Chung, S., additional, Shin, S. J., additional, Kim, H. W., additional, Chang, Y. S., additional, Kim, Y. S., additional, Park, C. W., additional, Takayanagi, K., additional, Hasegawa, H., additional, Shimizu, T., additional, Ikari, A., additional, Noiri, C., additional, Iwashita, T., additional, Tayama, Y., additional, Asakura, J., additional, Anzai, N., additional, Kanozawa, K., additional, Kato, H., additional, Mitarai, T., additional, Huang, M., additional, Ashour, R. H., additional, Fouda, A. E.-M. M., additional, Saad, M. A., additional, El-Banna, F. M., additional, Moustafa, F. A., additional, Fouda, M. I., additional, Sanchez-Nino, M. D., additional, Sanz, A. B., additional, Poveda, J., additional, Saleem, M., additional, Mathieson, P., additional, Ruiz-Ortega, M., additional, Selgas, R., additional, Egido, J., additional, Ortiz, A., additional, Soler, M. J., additional, Rebull, M., additional, Marquez, E., additional, Okazaki, S., additional, Kogure, Y., additional, Sano, T., additional, Hatano, M., additional, Kreft, E., additional, Kowalski, R., additional, Szczepansk-Konkel, M., additional, Liu, X., additional, Yang, G., additional, Osman, N. A., additional, NasrAllah, M. M., additional, Kamal, M. M., additional, Ahmed, A. I., additional, Fekih-Mrissa, N., additional, Mrad, M., additional, Baffoun, A., additional, Sayeh, A., additional, Hmida, J., additional, Gritli, N., additional, Galchinskaya, V., additional, Topchii, I., additional, Semenovykh, P., additional, Yefimova, N., additional, Zheng, D., additional, Hu, D., additional, Li, X., additional, Peng, A. I., additional, Olea-Herrero, N., additional, Arenas, M., additional, Munoz-Moreno, C., additional, Moreno-Gomez-Toledano, R., additional, Gonzalez-Santander, M., additional, Arribas, I., additional, and Bosch, R., additional

Published
2013
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11. Transplantation: basic science

Author

Cantaluppi, V., primary, De Lena, M., additional, Beltramo, S., additional, Ferrario, S., additional, Dellepiane, S., additional, Figliolini, F., additional, Bruno, S., additional, Biancone, L., additional, Segoloni, G. P., additional, Tetta, C., additional, Camussi, G., additional, Prasad, N., additional, Jaisawal, A., additional, Yadav, B., additional, Agarwal, V., additional, Tripathi, D., additional, Nunez-Lozano, R., additional, Quiros, Y., additional, Sanchez-Gonzalez, P., additional, Perez de Obanos, M. P., additional, Ruiz, J., additional, Lopez-Hernandez, F. J., additional, Lopez-Novoa, J. M., additional, Yang, J. W., additional, Kim, J. S., additional, Lee, J. Y., additional, Park, H. C., additional, Han, B. G., additional, Choi, S. O., additional, Matsuyama, M., additional, Yoshimura, R., additional, Hayama, T., additional, Chargui, J., additional, Touraine, J.-L., additional, Yoshimura, N., additional, Zanazzi, M., additional, Carta, P., additional, Caroti, L., additional, Antognoli, G., additional, Pinzani, P., additional, Salvianti, F., additional, Villari, D., additional, Minetti, E., additional, Genina, A., additional, Ismail, W., additional, Soliman, A., additional, Ucar, H., additional, Akbas, H. S., additional, Yilmaz, V. T., additional, Aktas, A., additional, Suleymanlar, G., additional, Yucel, G., additional, Cappuccilli, M. L., additional, La Manna, G., additional, Capelli, I., additional, Baraldi, O., additional, Cuna, V., additional, Battaglino, G., additional, Todeschini, P., additional, Feliciangeli, G., additional, Scolari, M. P., additional, Stefoni, S., additional, Loiacono, E., additional, Votta, B., additional, Amore, A., additional, Ranghino, A., additional, Camilla, R., additional, Peruzzi, L., additional, Donadio, M. E., additional, Serriello, I., additional, Gallo, R., additional, Puccinelli, M. P., additional, Coppo, R., additional, Sahin, G., additional, Meltem Akay, O., additional, Uslu, S., additional, Bal, C., additional, Ugur Yalcin, A., additional, Gulbas, Z., additional, and George, J., additional

Published
2013
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12. Transplantation basic

Author

Cantaluppi, V., primary, De Lena, M., additional, Figliolini, F., additional, Beltramo, S., additional, Medica, D., additional, Tognarelli, G., additional, Biancone, L., additional, Tetta, C., additional, Segoloni, G. P., additional, Camussi, G., additional, Pontrelli, P., additional, Cariello, M., additional, Verrienti, R., additional, Tataranni, T., additional, Gigante, M., additional, Loverre, A., additional, Stallone, G., additional, Schena, F. P., additional, Ranieri, E., additional, Gesualdo, L., additional, Grandaliano, G., additional, Coupel, S., additional, Charreau, B., additional, Canet, E., additional, Gerard, N., additional, Segalen, I., additional, Alexandre, N., additional, Grall, A., additional, Jacques-Olivier, P., additional, Hillion, S., additional, Le Meur, Y., additional, Alexandre, H., additional, Dany, A., additional, Michel, D., additional, Denis, G., additional, Christophe, L., additional, Nacera, O., additional, Isabelle, B., additional, Eric, R., additional, Yi Chun, D. X., additional, Buob, D., additional, Grimbert, P., additional, Glowacki, F., additional, Labalette, M., additional, Dufosse, F., additional, Nochy, D., additional, Copin, M.-C., additional, Boleslawski, E., additional, Noel, C., additional, and Hazzan, M., additional

Published
2012
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13. Il caracol quadrato in Sicilia (XVI secolo)

Author

Garofalo, Emanuela, Burgassi, V, Novelli, F, Spila, A, Bonino, M, Nobile, MR, Papa, I, Beltramo, S, D'Alessandro, R, Piccoli, E, Grimoldi, A, Landi, AG, Tocci, C, Gomez Serito, M, Ventura, G, Conforti, C, Bettini, S, Bulfone Gransinigh, F, Tabarrini, M, Natta, F, Pupi, E, Antista, A, Piazza, S, Nuccio, G, Forni, M, Cornaglia, P, Neri, G, Bartolozzi, C, Maspoli, R, Canella, G, Marzi, T, Florio, V, and Garofalo, Emanuela

Subjects
16th century, Square spiral staircase, stereotomy, Settore ICAR/18 - Storia Dell'Architettura, Sicily
Abstract

Square spiral staircases appear in Sicily during the 16th century in different contexts and for different purposes. This contribution focuses on two case studies at the “opposite poles” of a casuistry that shares the common characteristics of suspended ramps revolving within a square masonry box around a central void. By analysing these cases we find a probable link with models from Spain. At the same time, they demonstrate a common descent from a constructive culture rooted in the Spanish and Mediterranean stereotomic experience of the early modern age.

Published
2022

14. Le fondazioni dei frati predicatori in Sicilia tra XIII e XVIII secolo: un primo bilancio storiografico

Author

Piazza Stefano, Beltramo, S, Guidarelli, G, and Piazza Stefano

Subjects
Friar Preachers - religious architecture – Sicily –urban strategies –XIIIth-XVIIth centuries, Settore ICAR/18 - Storia Dell'Architettura
Abstract

The historiographical literature dedicated to the architecture of the Friar Preachers in Sicily, if one excludes the few studies on the complexes in Palermo, which are anyhow focussed on the churches only, is still substantially limited to the sporadic studies by local scholars or members of the Order on the history of the Dominicans in Sicily. The aim of our research, therefore, was to outline a general framework that could serve as a stepping stone for further research. It was first of all necessary to carry out a census of all the Dominican architectural complexes in Sicily, in order to determine the overall dimensions of the phenomenon and its chronological and territorial articulation, and then match it with the unfolding of historical events within the Order and the Kingdom’s socio-political context. Therefore, four main periods were identified with clear distinctive features: 1) from 1220 to 1250; 2) the 150-year period between the second half of the thirteenth century and the whole of the fourteenth century; 3) between the first decade of the fifteenth century and the 1580’s; and 4) between the seventeenth and the first half of the eighteenth centuries. Starting with this cognitive basis, the following analytical phase focussed on identifying the original architectural layout of the monastery complexes and any shared settlement strategies - in relation to the different periods of their construction and the different social forces involved (municipality or feudal nobility). In drawing an overall balance, it can be concluded that the spread of the Friar Preachers in Sicily, rather slow in the long initial phase spanning the thirteenth and fourteenth centuries, was a macroscopic phenomenon substantially linked to the fifteenth and sixteenth centuries. Moreover, the Order’s settlement took place following the logic of a widespread presence of small communities throughout the Kingdom, rather than through the construction of large complexes in major cities. The only exception to this logic was the Dominican community in Palermo.

Published
2021

15. Culture in dialogo attraverso e attorno la penisola italiana nei primi decenni del XVI secolo: il tardogotico e le altre opzioni

Author

GAROFALO, Emanuela, Alonso Ruiz, B, Rodríguez Estévez, JC, Jimenez Martin, A, Laguna Paul, T, Domenge Mesquida, J, Lopez Lorente, VD, Menéndez Gonzalez, N, Martinez de Simon, E, Nunes da Silva, R, Vidal Franquet, J, Lopez, I, Beltramo, S, Montana, S, Scaduto, F, Castro Santamaria, A, Cendon Fernandez, M, Pérez Monzon, O, Infante Limon, E, Vasallo Toranzo, L, Paulino Montero, E, Schirru, M, Scibilia, F, Ruiz Sousa, JC, Serra Desfilis, A, Garofalo, E, Olivares, D, Senent-Dominguez, R, Salcedo Galera, M, Calvo Lopez, J, Nobile, MR, Ibañez Fernández, J, Villaseñor Sebastian, F, Cuesta, J, Ojeda, A, Pinto Puerto, F, Ampliato Briones, A, Rabasa, E, Lopez Mozo, A, Gomez Martinez, J, Sobrino, M, Pérez, PP, Sauco, E, Martin Talaverano, R, Garcia Ortega, AJ, Alho, P, Palacios, JC, Tellia, F, Serrano Garcia, D, Ruiz de la Rosa, JA, Mora Vicente, G, Guerrero Vega, JM, and Gomez de Terreros, MV

Subjects
Italy, Rinascimento, Settore ICAR/18 - Storia Dell'Architettura, Tardogotico, Italia, Lategothic, Reinassance
Abstract

Dopo l’Unità d’Italia la storiografia si è concentrata nell’intero territorio nazionale sulla “ricerca” del Rinascimento, prescelto nel momento di costruzione di una identità nazionale come manifestazione artistica italiana per antonomasia. Tutto ciò ha originato una protratta “sfortuna critica” nei confronti di tutte quelle manifestazioni artistiche convenzionalmente riunite sotto l’etichetta di Tardogotico. In definitiva, se qualche indulgenza è stata concessa alla solida tradizione dei costruttori lombardi o agli ineludibili “esotismi” di una realtà rivolta a Oriente come quella veneziana, per il meridione peninsulare e le isole si è affermata l’idea di un ritardo culturale. Superati pregiudizi e preconcetti, la realtà che emerge anche per il contesto italiano è ben diversa e la presenza del Tardogotico appare meno marginale e circoscritta di quanto non risultasse sulla base di tendenziose selezioni. Ma si può parlare di una architettura tardogotica italiana? Di certo non esiste un fenomeno unitario e qui più che altrove la varietà delle linee di ricerca perseguite (strutturali, tecnologiche, formali, decorative) dà luogo a una grande varietà di esiti, confrontabili principalmente all’interno di contesti regionali ma con connotazioni che variano spesso da città a città. Nel complesso l’incidenza del Tardogotico nelle sue molteplici declinazioni appare territorialmente preponderante nel corso del XV secolo, restando la cultura rinascimentale limitata a pochi centri di sperimentazione. Nei primi decenni del Cinquecento il rapporto di forze in parte muta e, sotto la spinta di mode antiquarie, l’attenzione verso le forme del linguaggio all’antica inizia a farsi strada anche nelle “roccaforti” del gotico. Non si tratta tuttavia quasi mai di una netta scelta di campo o di uno scontro, ma piuttosto di un dialogo tra culture, che si risolve non di rado in fantasiose ibridazioni. Questo contributo analizza alcune declinazioni di questo dialogo attraverso una selezionata casistica di architetture prodotte in ambiti che ruotano intorno alla penisola italiana (Sardegna, Sicilia, Malta e Dalmazia) e nelle sue regioni meridionali, permeabili anche ad altre sollecitazioni provenienti da occidente e da oriente. Originating in historiographical trends of post-Unification Italy, the “quest” of the Renaissance and its credentials as the only manifestation of a progressive and specifically Italian culture, created in the Italian setting a prolonged period of “critical misfortune” in regard to all those artistic manifestations that were conventionally gathered together under the Late Gothic label. Indeed, if some leniency was granted to the solid tradition of the Lombard constructors or to the inescapable “exoticism” of a reality which looked East like Venice, as far as the Southern Italian mainland and islands were concerned, the idea of a cultural lag was asserted. After overcoming prejudice and preconceptions, the reality that emerges for also the Italian setting is well different, and the presence of the Late Gothic style appears less marginal and localized than we would expect on the basis of tendentious choices. But can we talk about an Italian Late Gothic architectural style? Certainly a unitary phenomenon does not exist and here more than anywhere else the variety of lines of research that have been pursued (structural, technological, formal, decorative) give rise to a great diversity of results, that are mainly comparable within regional contexts but whose connotations vary from city to city. On the whole, the incidence of Late Gothic in its multiple declensions appears territorially predominant over the course of the fifteenth century, with Renaissance culture limited to a few centres of experimentation. However, in the first few decades of the sixteenth century, the ratio of power changes partly and, under the thrust of antiquity , interest in the old-fashioned forms of language begins to rise from the ranks, even in the Gothic “strongholds”. Nevertheless it is hardly ever a question of a clear-cut choice of battleground or battle, but rather of a dialogue between cultures, which often results in imaginative hybridizations. Through a selection of cases of architecture in settings around the Italian peninsula (Sardinia, Sicily, Malta and Dalmatia) and in its Southern regions, but which are at the same time permeated by other solicitations from the west and the east, this paper looks at forms and types of dialogue between cultures that characterise the start of the “long” sixteenth century.

Published
2016

16. Isolation, characterization and potential role in beta cell-endothelium cross-talk of extracellular vesicles released from human pancreatic islets.

Author

Figliolini F, Cantaluppi V, De Lena M, Beltramo S, Romagnoli R, Salizzoni M, Melzi R, Nano R, Piemonti L, Tetta C, Biancone L, and Camussi G

Subjects
Blotting, Western, Endothelial Cells cytology, Flow Cytometry, Humans, MicroRNAs metabolism, RNA, Messenger metabolism, Reverse Transcriptase Polymerase Chain Reaction, Angiogenesis Inducing Agents metabolism, Cell Communication physiology, Endothelial Cells physiology, Insulin-Secreting Cells physiology, Receptor Cross-Talk physiology, Transport Vesicles metabolism
Abstract

The cross-talk between beta cells and endothelium plays a key role in islet physiopathology and in the revascularization process after islet transplantation. However, the molecular mechanisms involved in this cross-talk are not fully elucidated. Extracellular vesicles (EVs) are secreted membrane nanoparticles involved in inter-cellular communication through the transfer of proteins and nucleic acids. The aims of this study were: 1) isolation and characterization of EVs from human islets; 2) evaluation of the pro-angiogenic effect of islet-derived EVs on human islet endothelial cells (IECs). EVs were isolated by ultracentrifugation from conditioned medium of human islets and characterized by nanotrack analysis (Nanosight), FACS, western blot, bioanalyzer, mRNA/microRNA RT-PCR array. On IECs, we evaluated EV-induced insulin mRNA transfer, proliferation, resistance to apoptosis, in vitro angiogenesis, migration, gene and protein profiling. EVs sized 236±54 nm, expressed different surface molecules and islet-specific proteins (insulin, C-peptide, GLP1R) and carried several mRNAs (VEGFa, eNOS) and microRNAs (miR-27b, miR-126, miR-130 and miR-296) involved in beta cell function, insulin secretion and angiogenesis. Purified EVs were internalized into IECs inducing insulin mRNA expression, protection from apoptosis and enhancement of angiogenesis. Human islets release biologically active EVs able to shuttle specific mRNAs and microRNAs (miRNAs) into target endothelial cells. These results suggest a putative role for islet-derived EVs in beta cell-endothelium cross-talk and in the neoangiogenesis process which is critical for engraftment of transplanted islets.

Published
2014
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17. Protective effect of resin adsorption on septic plasma-induced tubular injury.

Author

Cantaluppi V, Weber V, Lauritano C, Figliolini F, Beltramo S, Biancone L, De Cal M, Cruz D, Ronco C, Segoloni GP, Tetta C, and Camussi G

Subjects
Adsorption, Aged, Cells, Cultured, Cytokines blood, Female, Humans, In Vitro Techniques, Inflammation Mediators blood, Kidney Diseases etiology, Kidney Diseases pathology, Male, Middle Aged, Sepsis complications, Cytokines isolation & purification, Inflammation Mediators isolation & purification, Kidney Diseases prevention & control, Kidney Tubules, Proximal pathology, Polymers pharmacology, Sepsis blood
Abstract

Introduction: A pro-apoptotic effect of circulating mediators on renal tubular epithelial cells has been involved in the pathogenesis of sepsis-associated acute kidney injury (AKI). Adsorption techniques have been showed to efficiently remove inflammatory cytokines from plasma. The aim of this study was to evaluate the efficiency of the hydrophobic resin Amberchrom CG161 M to adsorb from septic plasma soluble mediators involved in tubular injury., Methods: We enrolled in the study 10 critically ill patients with sepsis-associated AKI and we evaluated the effects of their plasma on granulocyte adhesion, apoptosis and functional alterations of cultured human kidney tubular epithelial cells. We established an in vitro model of plasma adsorption and we studied the protective effect of unselective removal of soluble mediators by the Amberchrom CG161 M resin on septic plasma-induced tubular cell injury., Results: Plasma from septic patients induced granulocyte adhesion, apoptosis and altered polarity in tubular cells. Plasma adsorption significantly decreased these effects and abated the concentrations of several soluble mediators. The inhibition of granulocyte adhesion to tubular cells was associated with the down-regulation of ICAM-1 and CD40. Resin adsorption inhibited tubular cell apoptosis induced by septic plasma by down-regulating the activation of caspase-3, 8, 9 and of Fas/death receptor-mediated signalling pathways. The alteration of cell polarity, morphogenesis, protein reabsorption and the down-regulation of the tight junction molecule ZO-1, of the sodium transporter NHE3, of the glucose transporter GLUT-2 and of the endocytic receptor megalin all induced by septic plasma were significantly reduced by resin adsorption., Conclusions: Septic plasma induced a direct injury of tubular cells by favouring granulocyte adhesion, by inducing cell apoptosis and by altering cell polarity and function. All these biological effects are related to the presence of circulating inflammatory mediators that can be efficiently removed by resin adsorption with a consequent limitation of tubular cell injury.

Published
2010
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18. Macrophage stimulating protein may promote tubular regeneration after acute injury.

Author

Cantaluppi V, Biancone L, Romanazzi GM, Figliolini F, Beltramo S, Galimi F, Camboni MG, Deriu E, Conaldi P, Bottelli A, Orlandi V, Herrera MB, Pacitti A, Segoloni GP, and Camussi G

Subjects
Aged, Animals, Case-Control Studies, Cell Culture Techniques, Cell Survival, Critical Illness, Humans, Mice, Mice, Inbred C57BL, Middle Aged, Acute Kidney Injury blood, Hepatocyte Growth Factor blood, Kidney Transplantation, Kidney Tubules physiology, Proto-Oncogene Proteins blood, Receptor Protein-Tyrosine Kinases blood, Regeneration physiology
Abstract

Macrophage-stimulating protein (MSP) exerts proliferative and antiapoptotic effects, suggesting that it may play a role in tubular regeneration after acute kidney injury. In this study, elevated plasma levels of MSP were found both in critically ill patients with acute renal failure and in recipients of renal allografts during the first week after transplantation. In addition, MSP and its receptor, RON, were markedly upregulated in the regenerative phase after glycerol-induced tubular injury in mice. In vitro, MSP stimulated tubular epithelial cell proliferation and conferred resistance to cisplatin-induced apoptosis by inhibiting caspase activation and modulating Fas, mitochondrial proteins, Akt, and extracellular signal-regulated kinase. MSP also enhanced migration, scattering, branching morphogenesis, tubulogenesis, and mesenchymal de-differentiation of surviving tubular cells. In addition, MSP induced an embryonic phenotype characterized by Pax-2 expression. In conclusion, MSP is upregulated during the regeneration of injured tubular cells, and it exerts multiple biologic effects that may aid recovery from acute kidney injury.

Published
2008
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