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In soybean, an environmentally stable male sterility system is vital for making hybrid seed production commercially viable. Eleven male-sterile, female-fertile mutants (ms1, ms2, ms3, ms4, ms5, ms6, ms7, ms8, ms9, msMOS, and msp) have been identified in soybean. Of these, eight (ms2, ms3, ms5, ms7, ms8, ms9, msMOS, and msp) have been mapped to soybean chromosomes. The objectives of this study were to (i) locate the msl, ms4, and ms6 genes to soybean chromosomes; (ii) generate genetic linkage maps of the regions containing these genes; and (iii) develop a comprehensive map of all known male-sterile, female- fertile genes in soybean. The bulked segregant analysis technique was used to locate genes to soybean chromosomes. Microsatellite markers from the corresponding chromosomes were used on [F.sub.2] populations to generate genetic linkage maps. The msl and ms6 genes were located on chromosome 13 (molecular linkage group F) and ms4 was present on chromosome 2 (molecular linkage group D1b). Molecular analyses revealed markers Satt516, BARCSOYSSR_02_1539, and AW186493 were located closest to msl, ms4, and ms6, respectively. The msl and ms6 genes, although present on the same chromosome, were independently assorting with a genetic distance of 73.7 cM. Using information from this study and compiled information from previously published male sterility genes in soybean, a comprehensive genetic linkage map was generated. Eleven male sterility genes were present on seven soybean chromosomes. Four genes were present in two regions on chromosome 2 (molecular linkage group D1b) and two genes were present on chromosome 13 (molecular linkage group F). Key words: Glycine max, male sterility, simple sequence repeat, genetic linkage map, male-sterile female-fertile. Chez le soya, un systeme de sterilite male presentant une stabilite environnementale serait un atout cle pour rendre commercialement viable la production de semence hybride. Onze mutants presentant une sterilite male et une fertilite femelle (ms1, ms2, ms3, ms4, ms5, ms6, ms7, ms8, ms9, msMOS et msp) ont ete identifies chez le soya. Parmi ceux-ci, huit (ms2, ms3, ms5, ms7, ms8, ms9, msMOS et msp) ont ete assignes a un chromosome. Les objectifs de ce travail etaient: (i) de situer les genes ms1, ms4 et ms6 sur les chromosomes du soya; (ii) de produire une carte genetique pour les regions contenant ces genes; et (iii) produire une carte complete de tous les genes conferant une sterilite male et une fertilite femelle chez le soya. Une analyse des segregants en composite (BSA pour << bulked segregant analysis >>) a ete employee pour assigner chacun des genes a un chromosome. Des marqueurs microsatellites logeant sur les chromosomes correspondants ont ete examines sur des populations [F.sub.2] pour produire des cartes genetiques. Les genes ms1 et ms6 ont ete assignes au chromosome 13 (groupe de liaison F) et ms4 a ete assigne au chromosome 2 (groupe de liaison D1b). Des analyses moleculaires ont revele que les marqueurs Satt516, BARSOYSSR_02_1539 et AW186493 etaient respectivement les marqueurs les plus proches des genes ms1, ms4 et ms6. Les genes ms1 et ms6, bien que presents sur le meme chromosome, presentaient une segregation independante et sont situes a 73,7 cM l'un de l'autre. En faisant appel a l'information produite au cours de ce travail et des etudes anterieures sur les genes de sterilite male, une carte complete a ete generee. Onze genes de sterilite male sont presents sur sept chromosomes. Quatre genes sont presents au sein de deux regions du chromosome 2 (groupe de liaison D1b)et deux genes sont presents sur le chromosome 13 (groupe de liaison F). [Traduit par la Redaction] Mots-cles: Gycine max, sterilite male, sequences repetees simples, carte genetique, male sterile femelle fertile., Introduction Male and female reproductive structures play an important role in the development of seeds in plants. Mutations in genes involved in development of stamens and (or) pistils can lead [...]