1. Investigation of Chlorophyllin in CHO-k1 (wild-type) and CHO-xrs5 (repair-deficient) Cells by a Chromosomal Aberration Assay
- Author
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Barenice Q. Jordão, Mávio S. Mantovani, Priscilla D. Negraes, and Veronica Elisa Pimenta Vicentini
- Subjects
Chlorophyllin ,Chinese hamster ovary cell ,Cell ,Wild type ,Methane sulfonate ,Cell Biology ,Plant Science ,Biology ,Molecular biology ,chemistry.chemical_compound ,Clastogen ,medicine.anatomical_structure ,chemistry ,Genetics ,medicine ,Cytotoxic T cell ,Animal Science and Zoology ,DNA - Abstract
Chlorophyllin, a sodium-copper salt derivative of chlorophyll a and b was evaluated at concentrations of 6.25, 12.5 and 25 μg/ml with regard to its clastogenic and anticlastogenic potential in Chinese hamster ovary cells CHO-k1 (wild-type) and CHO-xrs5 (DNA repair-defective), using a chromosomal aberration test. Cells were subjected to continuous exposure for 12 h to chlorophyllin in the presence or absence of ethyl methane sulfonate (EMS, 310 μg/ml). The results demonstrate that under the experimental conditions utilized, chlorophyllin was not cytotoxic nor clastogenic in either of the cell systems employed. Moreover, anticlastogenic activity was also not observed.
- Published
- 2003
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