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7. The ethylene-responsive transcription factor PpERF9 represses PpRAP2.4 and PpMYB114 via histone deacetylation to inhibit anthocyanin biosynthesis in pear

Author

Ni, Junbei, primary, Wang, Simai, additional, Yu, Wenjie, additional, Liao, Yifei, additional, Pan, Chen, additional, Zhang, Manman, additional, Tao, Ruiyan, additional, Wei, Jia, additional, Gao, Yuhao, additional, Wang, Dongsheng, additional, Bai, Songling, additional, and Teng, Yuanwen, additional

Published
2023
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16. PpyABF3 recruits the COMPASS‐like complex to regulate bud dormancy maintenance via integrating ABA signaling and GA catabolism.

Author

Yang, Qinsong, Wu, Xinyue, Gao, Yuhao, Ni, Junbei, Li, Jinjin, Pei, Ziqi, Bai, Songling, and Teng, Yuanwen

Subjects
DORMANCY in plants, BUDS, CATABOLISM, POLYMERASE chain reaction, WOODY plants, ABSCISIC acid, GIBBERELLIC acid
Abstract

Summary: Bud dormancy is essential for perennial trees that survive the cold winters and to flower on time in the following spring. Histone modifications have been reported to be involved in the control of the dormancy cycle and DAM/SVPs are considered targets. However, how the histone modification marks are added to the specific gene loci during bud dormancy cycle is still unknown.Using yeast‐two hybrid library screening and co‐immunoprecipitation assays, we found that PpyABF3, a key protein regulating bud dormancy, recruits Complex of Proteins Associated with Set1‐like complex via interacting with PpyWDR5a, which increases the H3K4me3 deposition at DAM4 locus.Chromatin immunoprecipitation‐quantitative polymerase chain reaction showed that PpyGA2OX1 was downstream gene of PpyABF3 and it was also activated by H3K4me3 deposition. Silencing of GA2OX1 in pear calli and pear buds resulted in a similar phenotype with silencing of ABF3. Furthermore, overexpression of PpyWDR5a increased H3K4me3 levels at DAM4 and GA2OX1 loci and inhibited the growth of pear calli, whereas silencing of PpyWDR5a in pear buds resulted in a higher bud‐break percentage.Our findings provide new insights into how H3K4me3 marks are added to dormancy‐related genes in perennial woody plants and reveal a novel mechanism by which ABF3 integrates abscisic acid signaling and gibberellic acid catabolism during bud dormancy maintenance. [ABSTRACT FROM AUTHOR]

Published
2023
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21. Ethylene mediates the branching of the jasmonate-induced flavonoid biosynthesis pathway by suppressing anthocyanin biosynthesis in red Chinese pear fruits

Author

Ni, Junbei, Zhao, Yuan, Tao, Ruiyan, Yin, Lei, Gao, Ling, Strid, Åke, Qian, Minjie, Li, Juncai, Li, Yuanjun, Shen, Jiaqi, Teng, Yuanwen, Bai, Songling, Ni, Junbei, Zhao, Yuan, Tao, Ruiyan, Yin, Lei, Gao, Ling, Strid, Åke, Qian, Minjie, Li, Juncai, Li, Yuanjun, Shen, Jiaqi, Teng, Yuanwen, and Bai, Songling

Abstract

Flavonoid accumulation in most fruitsis enhanced by ethylene and jasmonate. However, little is known about the hormone functions related to red pear fruit coloration or their combined effects and potential underlying mechanisms. Various treatments were used to investigate the flavonoid metabolite profile and pear transcriptome to verify the effects of ethylene and jasmonate on flavonoid biosynthesis in red pear fruits as well as the mechanism behind this. Ethylene inhibits anthocyanin biosynthesis in red Chinese pear fruits, whereas jasmonate increases anthocyanin and flavone/isoflavone biosyntheses. The branching of the jasmonate-induced flavonoid biosynthesis pathway is determined by ethylene. Co-expression network and Mfuzz analyses revealed 4,368 candidate transcripts. Additionally, ethylene suppresses PpMYB10 and PpMYB114 expression via TF repressors, ultimately decreasing anthocyanin biosynthesis. Jasmonate induces anthocyanin accumulation through transcriptional or post-translational regulation of TFs like MYB and bHLH in the absence of ethylene. However, jasmonate induces ethylene biosynthesis and the associated signaling pathway in pear, thereby decreasing anthocyanin production, increasing the availability of the precursors for flavone/isoflavone biosynthesis, and enhancing deep yellow fruit coloration. We herein present new phenotypes and fruit coloration regulatory patterns controlled by jasmonate and ethylene, and confirm that the regulation of fruit coloration is complex., Funding Agencies:Earmarked Fund for China Agriculture Research System CARS-28National Natural Science Foundation of China 31772272China Postdoctoral Science Foundation 2019M652095

Published
2020
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26. Ethylene mediates the branching of the jasmonate‐induced flavonoid biosynthesis pathway by suppressing anthocyanin biosynthesis in red Chinese pear fruits

Author

Ni, Junbei, primary, Zhao, Yuan, additional, Tao, Ruiyan, additional, Yin, Lei, additional, Gao, Ling, additional, Strid, Åke, additional, Qian, Minjie, additional, Li, Juncai, additional, Li, Yuanjun, additional, Shen, Jiaqi, additional, Teng, Yuanwen, additional, and Bai, Songling, additional

Published
2020
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27. Ethylene response factors Pp4ERF24 and Pp12ERF96 regulate blue light-induced anthocyanin biosynthesis in 'Red Zaosu' pear fruits by interacting with MYB114

Author

Ni, Junbei, Bai, Songling, Zhao, Yuan, Qian, Minjie, Tao, Ruiyan, Yin, Lei, Gao, Ling, Teng, Yuanwen, Ni, Junbei, Bai, Songling, Zhao, Yuan, Qian, Minjie, Tao, Ruiyan, Yin, Lei, Gao, Ling, and Teng, Yuanwen

Abstract

KEY MESSAGE: Pp4ERF24 and Pp12ERF96 fine tune blue light-induced anthocyanin biosynthesis via interacting with PpMYB114 and promoting the interaction between PpMYB114 and PpbHLH3, which enhances the expression of PpMYB114-induced PpUFGT. The red coloration of pear fruit is attributed to anthocyanin accumulation, which is transcriptionally regulated by the MYB-bHLH-WD40 complex. A number of ethylene response factors (ERF) have been identified to regulate anthocyanin biosynthesis in different plants. In pear, several ERF transcription factor genes were identified to be potentially involved in the light-induced anthocyanin biosynthesis according to transcriptome data. But the molecular mechanism of these ERFs underlying the regulation of anthocyanin accumulation is unknown. In this study, exposure of 'Red Zaosu' pear, a mutant of 'Zaosu' pear, to blue light significantly induced the anthocyanin accumulation by increasing the expression levels of anthocyanin biosynthetic genes. Gene expression analysis confirmed that the expression of Pp4ERF24 and Pp12ERF96 genes were up-regulated in the process of blue light-induced anthocyanin biosynthesis. Yeast two-hybrid and bimolecular fluorescence complementation assay revealed that Pp4ERF24 and Pp12ERF96 interacted with PpMYB114, but not with PpMYB10. Bimolecular fluorescence complementation assay demonstrated that the interaction between these two ERFs and PpMYB114 enhanced the interaction between PpMYB114 and PpbHLH3. Further analysis by dual luciferase assay verified that these two ERFs increased the up-regulation of PpMYB114-mediated PpUFGT expression. Furthermore, co-transformation of Pp12ERF96 with PpMYB114 and PpbHLH3 in tobacco leaves led to enhanced anthocyanin accumulation. Transient overexpression of Pp4ERF24 or Pp12ERF96 alone in 'Red Zaosu' pear fruit also induced anthocyanin biosynthesis in pear peel. Our findings provide insights into a mechanism involving the synergistic interaction of ERFs with PpMYB114 to regul, Funding Agencies:National Natural Science Foundation of China 31471852 31772272 Earmarked Fund for China Agriculture Research System CARS-28

Published
2019
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28. Changes in phytohormone content and associated gene expression throughout the stages of pear (Pyrus pyrifolia Nakai) dormancy.

Author

Ito, Akiko, Tuan, Pham Anh, Saito, Takanori, Bai, Songling, Kita, Masayuki, and Moriguchi, Takaya

Subjects
DORMANCY in plants, PLANT hormones, ABSCISIC acid, PEARS, GENE expression, INDOLEACETIC acid, GIBBERELLIC acid, MASS spectrometry
Abstract

To elucidate the role of phytohormones during bud dormancy progression in the Japanese pear (Pyrus pyrifolia Nakai), we investigated changes in phytohormone levels of indole acetic acid (IAA), gibberellic acid (GA), abscisic acid (ABA) and trans -zeatin (tZ). Using ultra-performance liquid chromatography/mass spectrometry/mass spectrometry, we monitored phytohormone levels in the buds of field-grown and potted trees that were artificially heated to modify the timing of dormancy and flowering (spring flush) progression. We also analyzed the expression of GA- and ABA-metabolic genes during dormancy. Indole acetic acid and tZ levels were low during dormancy and increased toward the flowering stage. Gibberellic acid levels were maintained at relatively high concentrations during the dormancy induction stage, then decreased before slightly increasing prior to flowering. The low GA concentration in potted trees compared with field-grown trees indicated that GA functions in regulating tree vigor. Abscisic acid levels increased from the dormancy induction stage, peaked near endodormancy release and steadily decreased before increasing again before the flowering stage. The ABA peak levels did not always coincide with endodormancy release, but peak height correlated with flowering uniformity, suggesting that a decline in ABA concentration was not necessary for resumption of growth but the abundance of ABA might be associated with dormancy depth. From monitoring the expression of genes related to GA and ABA metabolism, we inferred that phytohormone metabolism changed significantly during dormancy, even though the levels of bioactive molecules were consistently low. Phytohormones regulate dormancy progression not only upon the reception of internal signals but also upon sensing ambient conditions. [ABSTRACT FROM AUTHOR]

Published
2021
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33. Ethylene‐activated PpERF105 induces the expression of the repressor‐type R2R3‐MYB gene PpMYB140 to inhibit anthocyanin biosynthesis in red pear fruit.

Author

Ni, Junbei, Premathilake, Apekshika T., Gao, Yuhao, Yu, Wenjie, Tao, Ruiyan, Teng, Yuanwen, and Bai, Songling

Subjects
APPLES, ANTHOCYANINS, BIOSYNTHESIS, PEARS, STRAWBERRIES, FRUIT, TRANSCRIPTION factors
Abstract

Summary: Ethylene induces anthocyanin biosynthesis in most fruits, including apple (Malus domestica), strawberry (Fragaria × ananassa), and plum (Prunus spp.). However, ethylene inhibits anthocyanin biosynthesis in pear (Pyrus spp.), but the underlying molecular mechanism has not been characterized. In this study, ethylene induced the expression of PpERF105, which encodes a transcription factor. PpERF105 functioned as a transcriptional activator, but it inhibited anthocyanin biosynthesis in pear. A transcriptome analysis revealed that PpERF105 activated the expression of PpMYB140, which encodes an R2R3‐MYB transcriptional repressor. Moreover, PpMYB140 directly inhibited the expression of anthocyanin‐related structural genes. It also competed with PpMYB114 for the binding to bHLH3, ultimately resulting in the formation of the MYB140‐bHLH‐WDR complex rather than the conventional MBW complex, thereby further inhibiting anthocyanin biosynthesis. Furthermore, PpMYB140 prevented the overaccumulation of anthocyanins in the absence of ethylene. Collectively, our study data indicate that ethylene‐induced PpERF105 inhibits anthocyanin biosynthesis by upregulating PpMYB140 expression. Our findings may be useful for elucidating the molecular basis of the ethylene‐mediated inhibition of anthocyanin biosynthesis in fruit. Significance Statement: Ethylene induces anthocyanin biosynthesis in most fruits, including apple (Malus domestica), strawberry (Fragaria × ananassa), and plum (Prunus spp.), while it inhibits coloration in red pear (Pyrus spp.) fruit. In the present study, ethylene induced the expression of the transcription factor PpERF105, which activated the expression of PpMYB140, which further inhibited anthocyanin biosynthesis. These findings provide new insights into the molecular basis of the inhibitory effects of ethylene on anthocyanin biosynthesis in red pear fruit and enriches our knowledge about hormone‐regulated anthocyanin biosynthesis in fruit. [ABSTRACT FROM AUTHOR]

Published
2021
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34. Insertion of a transposon-like sequence in the 5′-flanking region of the YUCCA gene causes the stony hard phenotype

Author

Tatsuki, Miho, primary, Soeno, Kazuo, additional, Shimada, Yukihisa, additional, Sawamura, Yutaka, additional, Suesada, Yuko, additional, Yaegaki, Hideaki, additional, Sato, Akiko, additional, Kakei, Yusuke, additional, Nakamura, Ayako, additional, Bai, Songling, additional, Moriguchi, Takaya, additional, and Nakajima, Naoko, additional

Published
2018
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36. Distribution of MdACS3 null alleles in apple (Malus × domestica Borkh.) and its relevance to the fruit ripening characters

Author

Bai, Songling, Wang, Aide, Igarashi, Megumi, Kon, Tomoyuki, Fukasawa-Akada, Tomoko, Li, Tianzhong, Harada, Takeo, and Hatsuyama, Yoshimichi

Subjects
breeding, ethylene, food and beverages, apple, allelotypes, Research Papers, MdACS3, ripening
Abstract

Expression of MdACS3a, one of the ripening-related ACC synthase genes, plays a pivotal role in initiating the burst of ethylene production by MdACS1 in apple fruit. Although previous studies have demonstrated the presence of MdACS3a-null alleles through deficiency of transcription activity or loss of enzyme activity due to amino acid substitution, which may affect the storage properties of certain fruit cultivars, an overall picture of these null alleles in cultivars is still lacking. The present study investigated the distribution of null allelic genes in 103 cultivars and 172 breeding selections by using a simple sequence repeat (SSR) marker linked to them. The results indicated that both allelic genes were widely distributed throughout the examined cultivars and selections, some occurring as the null genotype, either homozygously or heterozygously, with each null allele. The implications of MdACS3a distribution results and the influence of its null allelotypes in fruit characters are discussed.

Published
2012

50. Light-responsive transcription factor PpWRKY44 induces anthocyanin accumulation by regulating PpMYB10expression in pear

Author

Alabd, Ahmed, Ahmad, Mudassar, Zhang, Xiao, Gao, Yuhao, Peng, Lin, Zhang, Lu, Ni, Junbei, Bai, Songling, and Teng, Yuanwen

Abstract

Anthocyanins are a valuable source of antioxidants in the human diet and contribute to fruit coloration. In red-skinned pears, anthocyanin biosynthesis can be induced by light, in which the MYB–bHLH–WDR complex plays a critically important role in transcriptional regulation. However, knowledge of WRKY-mediated transcriptional regulation of light-induced anthocyanin biosynthesis is scarce in red pears. This work identified and functionally characterized a light-inducing WRKY transcription factor, PpWRKY44, in pear. Functional analysis based on overexpressed pear calli showed that PpWRKY44 promoted anthocyanin accumulation. Also, transiently overexpressed PpWRKY44in pear leaves and fruit peels significantly enhanced the accumulation of anthocyanin, whereas silencing PpWRKY44in pear fruit peels impaired induction of the accumulation of anthocyanin by light. By chromatin immunoprecipitation and electrophoretic mobility shift assay coupled to a quantitative polymerase chain reaction, we found that PpWRKY44 bound in vivoand in vitroto the PpMYB10promoter, revealing it as a direct downstream target gene. Moreover, PpWRKY44was activated by PpBBX18, a light signal transduction pathway component. Our results explained the mechanism mediating the impacts of PpWRKY44 on the transcriptional regulation of anthocyanin accumulation, with potential implications for fine-tuning the fruit peel coloration triggered by light in red pears.

Published
2022
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