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Simple Summary: This study tested hand warmers as oxygen scavengers for controlling insect pests in hermetic storage by accelerating oxygen depletion. Using one, two, or four hand warmers, this study found that only four hand warmers effectively reduced oxygen below 1% and maintained it for up to 168 h. Insect mortality increased with more hand warmers and extended storage duration, achieving 100% mortality after 5 and 8 days for four and two hand warmers, respectively. Hand warmer treatments did not affect the moisture content or germination rates of cowpea seeds. Hand warmers effectively accelerate oxygen depletion, leading to high insect mortality and progeny suppression, without impacting grain quality. Accelerating oxygen depletion during hermetic storage can minimize pest damage and preserve product quality. This study evaluated the effectiveness of hand warmers in accelerating hypoxia to control insect pests inside hermetic containers. We used one, two, or four hand warmers to deplete oxygen in a 4-gallon hermetic jar with 4 kg of cowpea and cowpea bruchids, alongside a non-hermetic control with cowpea bruchids and no hand warmers. Oxygen levels, insect mortality, egg counts, seed moisture content, and germination rates were monitored over 2, 5, or 8 days of storage. Only the four hand warmers treatment reduced oxygen levels below 1% within 12 h and maintained them for up to 168 h. The other treatments did not achieve this level. Insect mortality was higher with more hand warmers and extended storage duration, reaching 100% after 5 and 8 days with four and two hand warmers, respectively. Similarly, increased hand warmers and extended storage durations reduced egg counts and adult emergence. The treatments did not affect the moisture content or germination rates of the stored cowpea seeds. Hand warmers proved effective in accelerating hypoxia during hermetic storage, resulting in high insect mortality and reduced reproduction, without compromising grain quality. [ABSTRACT FROM AUTHOR]

A morphological study is carried out on the taxonomic characters of the species of the genus Bruchidius Schilsky, 1905, grouped in the bimaculatus subgeneric group, present in the Iberian Peninsula (Spain and Portugal), the Balearic Islands, and the Canary Islands. The morphological characters and their value as a discriminating criterion of the species of the group are analyzed, as well as the concordant and discordant aspects of the known trophic biology of the different species. [ABSTRACT FROM AUTHOR]

Copyright of Baghdad Science Journal is the property of Republic of Iraq Ministry of Higher Education & Scientific Research (MOHESR) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Rice straw, a byproduct of harvesting rice, must be disposed of by farmers in a variety of ways, including burning, which is hazardous for the environment. To address this issue, the straw needs to be utilized and turned into valuable products. One such product is nano-silica (SNPs), which will be synthesized and investigated in our study as a safe alternative to chemical insecticides. Rice straw-derived SNPs were synthesized using the Sol–Gel method. The contact toxicity of SNPs on Callosobruchus maculatus, a major pest of cowpea seeds, has been assessed. The size of synthesized SNPs was determined by transmission electron microscopy to be ~ 4 nm. The SNPs estimated LC50 on C. maculatus adults was 88.170 ppm after 48h exposure. By raising the tested concentration, SNPs treatment increased the mortality%, which reached 100% at 200 ppm exposures. Additionally, SNPs at LC50 treatment decreased adult longevity and the average number of emerged adults. The findings also verified that SNPs had no phytotoxic effects on the cowpea seeds germination. Rather, their application improved seed germination efficacy. This study proposed that rice straw can be utilized to manufacture highly efficient SNPs which can be efficiently employed to preserve stored grains from C. maculatus infestation. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Plant Production is the property of Egyptian National Agricultural Library (ENAL) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

A morphological study is carried out on the taxonomic characters of the species of the genus Bruchidius Schilsky, 1905, grouped in the foveolatus subgeneric group, present in the Iberian Peninsula (Spain and Portugal), the Balearic Islands, and the Canary Islands. The morphological characters and their value as a discriminating criterion of the species of the group are analyzed, as well as the concordant and discordant aspects of the known trophic biology of the different species. [ABSTRACT FROM AUTHOR]

Mungbean seeds [Vigna radiata (L.)] are easily damaged by insects during storage, and essential oils (EOs) have proved effective for controlling insect infestation. This research study assessed the bioactivity of EO from Jasminum sambac (L.) Aiton against Callosobruchus maculatus (F.). The chemical composition of EO was analysed by a gas chromatograph-mass spectrometer (GC-MS). All experiments were conducted following a completely randomised design (CRD) with four replications of the residual contact and seed dressing bioassays. Results indicated that J. sambac EO contained 35 compounds (97.587%), with the main component 3-hexen-1-ol benzoate. Residual contact toxicity LC50 values of this EO on adult C. maculatus at 24, 48, and 72 h were 5.01, 4.00, and 3.13 µL/mL, respectively. J. sambac EO at 8 µL/L showed the highest residual contact efficacy against C. maculatus, killing up to 100% in 72 hours. The highest concentration of J. sambac EO (1 mL/kg) gave optimal protection against C. maculatus within 4 and 5 days with 100% mortality. This concentration showed 100% inhibition of adult F1 progeny emergence, with no seed damage and weight loss of V. radiata L. after 30 days of exposure, and not significantly different from 0.8 mL/kg. Results indicated that J. sambac EO effectively prevented C. maculatus infestation and it could be used as an alternative to commercial chemical insecticides. [ABSTRACT FROM AUTHOR]

The pulse beetle is a field-to-store pest as its infestation on pulses often begins in the field itself as adults lay eggs on mature pods and when such seed is harvested and stored, the pest population increases rapidly and results in total destruction within a short period of 3-4 months. Keeping in view, varietal screening of fifty chickpea genotypes was carried out in the storage laboratory, Department of Entomology, OUAT, BBSR and the performance of the genotypes was assessed based on various biological parameters of test insect, damage and infestation by C. chinensis. The results indicated that none of the genotypes was completely resistant to pest attack whereas 4 genotypes (Himachal Chana 1, Dheera (NBeG-47), JG-14 and Dilaji) were found moderately resistant, 8 genotypes (Phule Vikram, JG 11, ICCV-181108, ICCV-181107, ICCV-181605, C-18203, C-18205 and C-18252) were moderately susceptible, 11 genotypes (RVG-204, RVG-203, JAKI-9218, Pratap Chana, Bharati, ICCC 4, ICCV-181106, ICCV-181612, C-18206, ICCV-181101 and Radhey) were susceptible and 27 genotypes (NBeG-49, Himachal Chana 2, JG-16, JG-130, CO 4, Vishal, Kranthi, NBeG-3, ICCV-14102, ICCV-171117, C-18175, ICCV-181611, ICCV-14106, Kalahandi Local, ICC 3137, ICCL 86111, C-19162, C-19168, GNG 2207, BG 3043, GG 3, Birsa Chana 3, C 19199, RSG 963, C 19200, KPG 59 and NBeG 119) were noticed to be highly susceptible. The bio-chemical constituents analyzed in the present studies viz., protein, phenol, ash and fibre contents of the genotypes contributed to the resistance / susceptibility of C. chinensis. Among the biochemical parameters, protein exerted significant positive effect whereas phenol, ash and fibre contents exhibited negative influence on pest infestation and development. [ABSTRACT FROM AUTHOR]

The present investigation was undertaken in 110 mung bean genotypes in alpha lattice design in two replications to estimate the correlation coefficients, direct and indirect effects of various yield contributing traits on grain yield for 18 quantitative traits and nine screening traits against bruchids. The correlation coefficient analysis revealed that single plant yield showed high significant positive correlation with number of pods per plant, followed by number of clusters per plant and hundred seed weight. Path analysis showed that days to 50 per cent flowering and number of pods per plant showed high positive direct effect on single plant yield. The bruchid bioassay resulted in the identification of 12 genotypes with complete resistance to bruchids and all the characters showed high significant positive correlation with each other except for number of eggs laid. Hence, the resistant genotypes identified in this study can be used in resistance breeding programme for bruchids. [ABSTRACT FROM AUTHOR]

Copyright of Revista CienciaUAT is the property of Universidad Autonoma de Tamaulipas and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

A study on the taxonomic characters that have been used to discriminate the different species of the genus Bruchidius included in the unicolor group, represented in the Iberian-Balearic and Canary Islands fauna, is carried out. The taxonomic and biological heterogeneity of the species included in this group is discussed and some criteria are provided for characterization according this heterogeneity. The morphological analysis concludes with the low taxonomic value of its characters to differentiate the species, requiring the use of a set of characters. [ABSTRACT FROM AUTHOR]

Bruchids are the most important pests of leguminous seeds in the world. In this study, the focus was done on Callosobruchus maculatus, a serious pest of Vigna unguiculata seeds. As no efficient control methods preventing collateral effects on beneficials currently exist, this study investigated whether RNA interference (RNAi) could provide a new biotechnological and selective tool for bruchids control. Three principal objectives were followed including (i) the identification of all RNAi machinery core components and a key protein to silence in C. maculatus genome (c.f., dicer-2, argonaute-2, R2D2, and laccase 1), (ii) the identification of suitable reference gene for RT-qPCR analyses, and (iii) the micro-injection of dsRNA coding for laccase 1 to adults of C. maculatus to assess gene expression levels by RT-qPCR and potentially related mortalities. Phylogenetical analyses performed from transcriptomic information successfully identified all necessary proteins in the RNAi mechanism and also the open reading frame of laccase 1 in C. maculatus. A new reference gene was identified (i.e., alpha-tubuline 1) and coupled with glutiathone S transferase for RT-qPCR analyses. Double-stranded RNAs coding for laccase 1 and green fluorescent protein (control) were produced and 400 ng of each dsRNA were micro-injected into C. maculatus adults. RT-qPCR analyses revealed a stable significant decrease in laccase 1 expression in about 80% of adults treated with laccase 1 dsRNA after three days post-injection. No significant mortalities were observed which is probably related to the non-exposure of adults to anti-nutritional factors that are usually regulated by laccase. Further research should focus either on the feeding larval stage which is directly exposed to anti-nutritional factors, or on other target genes to induce dead phenotypes. This study is the first gene silencing report on a bruchid species and supports RNAi as a potential future method of control. [ABSTRACT FROM AUTHOR]

Pulse beetle is the most harmful pest attacking stored grains and affecting quality and marketability. Continuous use of chemical-based pesticides against pulse beetle led to the development of insecticidal resistance; essential oils (EOs) can be an effective natural alternative against this pest. The main objective was to study the chemical composition of seven EOs viz., Acorus calamus, Hedychium spicatum, Lavandula angustifolia, Juniperus recurva, Juniperus communis, Cedrus deodara and Pinus wallichiana, their insecticidal and enzyme inhibition activities against pulse beetle. The primary compounds present in these EOs were cis-asarone, 1,8-cineole, linalyl isobutyrate, 2-β-pinene, camphene, α-dehydro-ar-himachalene and camphene. A. calamus oil showed promising fumigant toxicity to Callosobruchus maculatus and C. chinensis (LC50 = 1357.86 and 1379.54 µL/L, respectively). A combination of A. calamus + L. angustifolia was effective against C. maculatus and C. chinensis (LC50 = 108.58 and 92.18 µL/L, respectively). All the combinations of EOs showed synergistic activity. In the repellency study, A. calamus showed more repellence to C. maculatus and C. chinensis (RC50 = 53.98 and 118.91 µL/L, respectively). A. calamus and L. angustifolia oil at 2500, 5000 and 10,000 µL/L significantly inhibited the AChE and GST enzymes in C. maculatus and C. chinensis after 24 and 48 h. [ABSTRACT FROM AUTHOR]

The present study evaluated the effect of extracts of three spices in control of Callosobruchus subinnotatus (Pic). The experimental design used was completely randomized design (CRD). Ginger, scent leaf and curry leaf were obtained and screened for phytochemical and proximate composition using standard laboratory techniques. The Bambara nut was subjected to three treatments (To, T1, T2 and T3) which were replicated 4 times. To was used as control while the rest were treated with the spices. Results from the study showed that the highest mortality rate was recorded on 100g of Ginger extract having percentage mortality of 93.33% (T3). It was followed by scent leaf with a mortality rate of 43.33% (T1). The result also showed that Ginger powder extract gave the lower mean number of holes (41) and with lower weight loss of 4.9% of nuts amongst the extracts used. Bambara nuts that were not treated with plant spice extracts (To) gave the highest mean number of holes or punctures (211) and the highest weight loss 25.5% of nuts. Conclusion of this study is that the plant extracts were effective at controlling Callosobruchus subinnotatus, though at varying degrees. These biopesticides are part of humans and animals food, often are used as ethno-medicines, and are more environmentally friendly being biodegradable, so all these things recommend to be used in the detriment of chemical pesticides. [ABSTRACT FROM AUTHOR]

This work aimed to evaluate the efficiency of traditional method of storage of cowpea grains and seeds for the control of the Callosobruchus maculatus (Fabr.) (Coleoptera: Bruchidae). The storage method consists of packing grains and seeds, mixed with threshing residue, in hermetically sealed polyethylene bottles or barrels. In the experiment, four treatments were evaluated: (i.) Perforated flask containing grains, (ii.) Non-perforated flask containing grains, (iii.) Perforated flask containing a mixture of grains and threshing residues and (iv.) Non-perforated bottle containing a mixture of grains and threshing residues, the latter representing the traditional method (control). For each treatment, 4 replicates were used. For each pot, 20 adult individuals of the insect-pest were inserted. After induced infestation, the pots were stored in a place protected from rain and sun, simulating the reality of farmers' storage. At 35 and 70 days after the induced infestation, the following variables were counted for each pot: number of live insects, number of dead insects, number of holes caused by insects in the grains and percentage of grains attacked. The use of threshing residue was not efficient for the control. On the other hand, the absence of gas exchange in the pot decreased the multiplication of the insect pest, indicating the importance of using hermetic barrels ourvacuum packaging for storage and commercialization. [ABSTRACT FROM AUTHOR]

The pulse beetle Callosobruchus maculatus Fabricius (Coleoptera: Bruchidae) is an important pest of stored soybean grains. It is imperative to screen out the prevailing genotypes of Beninese soybean cropping systems in order to find out the resistant ones against C. maculatus infestations. The effect of seven grain physical traits (testa thickness, colour, texture, hardness, length, breadth, and 100-grain mass) on the susceptibility of eight soybean varieties to C. maculatus were evaluated in the laboratory. The correlations and contributions of the studied traits were evaluated using correlation path coefficient analysis. The tested soybean varieties showed a variation in physical seed characteristics. A differential susceptibility of soybean varieties to C. maculatus was observed, with the white seeded variety Whéwhé having longest and largest grains was the most susceptible. Based on the Dobie susceptibility index, the Yovoton variety was proved to be resistant to C. maculatus attacks. While, Kecheke, Houeton, Adjaton and Vovoh varieties were classified as moderately resistant to C. maculatus. The correlation analysis indicated that 100-seed weight had significant positive correlation with F1 progeny (r = 0.439), seed consumption (r = 0.467), number of eggs laid (r = 0.295) and susceptibility index (r = 0.453). Path coefficient analysis showed that each seed physical character and its interactions with the others characters influenced soybean grains susceptibility to C. maculatus. Soybean seed thickness showed the higher direct positive effect on soybean susceptibility to C. maculatus indicating that breeding should be done based on this trait to improve soybean seed resistance. [ABSTRACT FROM AUTHOR]

The pulse beetle Callosobruchus maculatus Fabricius (Coleoptera: Bruchidae) is an important pest of stored soybean grains. It is imperative to screen out the prevailing genotypes of Beninese soybean cropping systems in order to find out the resistant ones against C. maculatus infestations. The effect of seven grain physical traits (testa thickness, colour, texture, hardness, length, breadth, and 100-grain mass) on the susceptibility of eight soybean varieties to C. maculatus were evaluated in the laboratory. The correlations and contributions of the studied traits were evaluated using correlation path coefficient analysis. The tested soybean varieties showed a variation in physical seed characteristics. A differential susceptibility of soybean varieties to C. maculatus was observed, with the white seeded variety Whéwhé having longest and largest grains was the most susceptible. Based on the Dobie susceptibility index, the Yovoton variety was proved to be resistant to C. maculatus attacks. While, Kecheke, Houeton, Adjaton and Vovoh varieties were classified as moderately resistant to C. maculatus. The correlation analysis indicated that 100-seed weight had significant positive correlation with F1 progeny (r = 0.439), seed consumption (r = 0.467), number of eggs laid (r = 0.295) and susceptibility index (r = 0.453). Path coefficient analysis showed that each seed physical character and its interactions with the others characters influenced soybean grains susceptibility to C. maculatus. Soybean seed thickness showed the higher direct positive effect on soybean susceptibility to C. maculatus indicating that breeding should be done based on this trait to improve soybean seed resistance. [ABSTRACT FROM AUTHOR]

A detailed and illustrated description of Bruchidius misellus (Boheman, 1833), is offered, being a poorly known species, which has always been considered as a variety of other species of the unicolor group, but currently there is scientific consensus in considering it as a good species, with distribution restricted to a few countries of the Western Mediterranean, including those of the Iberian Peninsula. [ABSTRACT FROM AUTHOR]

A study on the taxonomic characters that have been used to discriminate the different species of the genus Bruchidius included in the varius group, represented in the Iberian-Balearic and Canary Islands fauna, is carried out. The taxonomic and biological heterogeneity of the species included in this group is discussed and some criteria are provided for characterization according to this heterogeneity. [ABSTRACT FROM AUTHOR]

Callosobruchus maculatus is one of the most important pests of cowpea in storage causing severe economic damage to the grain. This study investigated the efficacies of three plant materials (Azadirachta indica, Calotropis procera and Chromolaena odorata) leaves against the cowpea weevil. Concentrations of 0.1, 0.25 and 0.5g of the plant powders were used on 10g of grains with 10 adult weevils in each and a Control (untreated) in triplicates. The results showed significant (P< 0.05) negatively effects of the plant materials on the survival of C. maculatus at the highest concentration. In all trails, mean daily mortality in adult C. maculatus were significantly(p<0.05) increased. All plant powder type were effective but concentration-dependent, with C. procera recording significantly (P < 0.05) higher mortality at the various concentrations while C. odorata, elicited the least mean daily mortality. The lowest LD50 (0.63g) was obtained with C. procera. These plants materials were found to also affect the egg-laying capacity of C. maculatus. Treatment with C. odorata recorded significantly(P<0.05) higher number of eggs laid at all concentrations, though the egg-laying capacity was also concentration-dependent; whereas C. procera recorded the least number of eggs laid. All the three plants powders tested demonstrated significant insecticidal potency on stored cowpea weevils, with C. procera and C. odorata showing significantly higher and lower insecticidal potentials respectively. These findings will help in solving problem associated with food security especially with respect to stored produce. [ABSTRACT FROM AUTHOR]

Mungbean (Vigna radiata) is an important short-season legume widely cultivated in Asia, particularly India. It is highly susceptible to bruchids and developing bruchid resistance is an important goal in mungbean breeding programs. In the present study, 52 mungbean genotypes were evaluated for bruchid resistance based on the "no-choice test" and identified two highly resistant genotypes (V2802BG and V2709) with no adult emergence and seed damage. Further, these two resistant genotypes were crossed with six high-yielding bruchid susceptible cultivars (CO 5, CO 6, CO 7, CO 8, VBN 2, and VBN 3), and 12 independent F1 populations were generated. Of these, one population derived from CO 6 × V2802BG was selected (based on the good combining ability of the parents) and forwarded to later generations to trace the bruchid-resistant lines. A total of 159 F2:3 families were screened for bruchid resistance, and the results showed that seven families were highly resistant, whereas the remainder were resistant to highly susceptible. Further, those seven families were evaluated in F4 and F5 generations. As a result, five highly resistant lines (BSR-GG-1-49-3-1, BSR-GG-1-56-2-2, BSR-GG-1-160-5-3, BSR-GG-1-170-2-4, and BSR-GG-1-198-1-4) with good agronomic performances were identified. The newly developed lines could be tested in multi-location trials and then be utilized as a potential source of genetic material for improving the bruchid resistance in mungbean breeding programs. [ABSTRACT FROM AUTHOR]

Mung bean is vulnerable to bruchids (Callosobruchus spp.), resulting in low quality and losses worldwide. Developing resistant cultivars is the most effective, economical, and eco-friendly way to protect mung bean seeds from the damage of bruchids. Previously, we identified two gene loci that are tightly linked with bruchid resistance, which makes new cultivar development possible using molecular assisted selection (MAS). In the present study, marker-assisted backcross (MABC) breeding was employed to introgress the bruchid resistance gene VrPGIP2 locus from the donor parent V2802 into a popular cultivar, Kamphaeng Saen 1. Markers VrBR-SSR013 and DMB-SSR158 were used for foreground selection, and phenotypic selection was used for background selection. Three advanced inbred lines (R67-11, R67-22, and R92-15) carrying the resistance gene VrPGIP2 were developed from the BC3F6 population. Further analysis showed that the line R67-22 performed best; it was highly resistant to bruchids and showed excellent agronomic traits in the field. Therefore, R67-22 could be further evaluated in large-scale trials for release as a variety. [ABSTRACT FROM AUTHOR]

Knowledge about biology of seed beetles (Coleoptera, Bruchidae) is scarce or non-existent in the vast majority of species of no economic interest. Data on trophic biology are confusing, mixing host species with refuge species. In recent years, an effort has been made to understand the biology of these insects, based on in vivo information. In this article we present observations on the trophic and reproductive biology of six species of the genus Bruchidius, made in the course of these studies. [ABSTRACT FROM AUTHOR]

Cowpea (Vigna unguiculata) is an important legume which is consumed globally for protein intake, particularly in Asian states. It is a well-known source of dietary fiber, protein, minerals, and vitamins. The cowpea grains are stored after harvest and used till the next harvest. However, the grains are infested by storage pests, primarily Callosobruchus maculatus. Hence, effective management strategies are needed to protect the stored grains form the pests. This study assessed the efficacy of some edible oils in suppressing C. maculatus infestation in stored cowpea grains. Four different botanical oils (i.e., mustard, neem, poppy, and pumpkin) at four different concentrations (i.e., 0.5, 1.0, 1.5 and 2.0 ml per 100 g grain) were included in the study. A control treatment without any botanical oil was also included for comparison. The relevant concentrations of botanical oils were poured into plastic containers containing 100 g cowpea grains and ten C. maculatus adults were released. The jars were sealed and placed at room temperature. Data relating to mortality, oviposition, F1 adult emergence, and seed weight loss were recorded. The tested botanical oils and their concentrations significantly affected mortality after one day. Mortality after 2nd and 3rd days remained unaffected by botanical oils and their different concentrations. The highest mortality was recorded in neem oil-treated grains followed by poppy, pumpkin, and mustard oils. Increased oviposition rate was observed in the grains treated with mustard and pumpkin oils, while those treated with neem and poppy oil recorded decreased oviposition. The control treatment had increased oviposition rate compared to tested botanical oils. All botanical oils significantly inhibited egg laying percentage. The highest germination was recorded for the grains treated with mustard oil followed by pumpkin, poppy, and neem oils, respectively. The lowest germination was recorded for control treatment. Significant differences were noted for C. maculatus repellency among botanical oils. No emergence of adults (F1 progeny) was recorded in all tested botanical oils; thus, F1 progeny was inhibited by 100%. Weight loss, damage percentage, and holes in the grains were not recorded since F1 progeny did not emerge. It is concluded that tested botanical oils are promising and could be utilized to control C. maculatus in cowpea grains during storage. [ABSTRACT FROM AUTHOR]

Background: The cowpea bruchid, Callosobruchus maculatus is the major postharvest insect pest of cowpea seeds in storage. This had led to huge losses and quality deterioration of stored cowpea seeds that serve as poor man's meat in lieu of expensive meat source in developing countries. This research was carried out to evaluate the bioefficacy of rice husk, maize cob, groundnut, and cowpea pods against C. maculatus in the laboratory. Adult insect mortality, eggs laid, adult emergence, damaged seeds, weight loss and beetle perforation index (BPI) were evaluated. Phytochemicals of the wastes were investigated. Results: The result showed that the agricultural wastes contained alkaloids (1.56–2.77 mg/g), saponin (1.51–3.38 mg/g), phytate (7.00–17.76 mg/g), oxalate (0.32–1.13 mg/g). All agricultural wastes showed a high mortality effect on C. maculatus, and their effects increased as the exposure time and concentration/ dosage increased. Beetle mortality was highest in cowpea pod with 80% mortality for powder after 3 and 4 days when applied at 0.5 g/20 g cowpea seed. This showed that cowpea pod powder has the greatest insecticidal activity while the least was observed in maize cob powder (73.33%). Fewer adults emerged with maize cob having the least emergence when applied at 0.2–0.5 g dosage. Extracts of all agricultural wastes tested against C. maculatus were able to affect 70–100% mortality after 4 days of application at concentration 0.5 ml with cowpea pod extract causing 100% mortality of beetle after 4 days of application. The calculated lethal dose (LD50 and LD90) and concentrations (LC50 and LC90) of wastes powders and extracts cowpea pod was observed to have the lowest lethal dose while maize cob wastes were the highest across all period of exposure. Conclusion: Base on the results obtained, cowpea pod waste was the most toxic in biocontrol of C. maculatus. [ABSTRACT FROM AUTHOR]

A study on the taxonomic characters that have been used to discriminate the different species of the genus Bruchidius included in the seminarius group, represented in the Iberian-Balearic and Canary Islands fauna, is carried out. The taxonomic and biological heterogeneity of the species included in this group is discussed and some criteria are provided for characterization according to this heterogeneity. [ABSTRACT FROM AUTHOR]

Bruchids are the most important pests of leguminous seeds in the world. In this study, the focus was done on Callosobruchus maculatus, a serious pest of Vigna unguiculata seeds. As no efficient control methods preventing collateral effects on beneficials currently exist, this study investigated whether RNA interference (RNAi) could provide a new biotechnological and selective tool for bruchids control. Three principal objectives were followed including (i) the identification of all RNAi machinery core components and a key protein to silence in C. maculatus genome (c.f., dicer-2, argonaute-2, R2D2, and laccase 1), (ii) the identification of suitable reference gene for RT-qPCR analyses, and (iii) the micro-injection of dsRNA coding for laccase 1 to adults of C. maculatus to assess gene expression levels by RT-qPCR and potentially related mortalities. Phylogenetical analyses performed from transcriptomic information successfully identified all necessary proteins in the RNAi mechanism and also the open reading frame of laccase 1 in C. maculatus. A new reference gene was identified (i.e., alpha-tubuline 1) and coupled with glutiathone S transferase for RT-qPCR analyses. Double-stranded RNAs coding for laccase 1 and green fluorescent protein (control) were produced and 400 ng of each dsRNA were micro-injected into C. maculatus adults. RT-qPCR analyses revealed a stable significant decrease in laccase 1 expression in about 80% of adults treated with laccase 1 dsRNA after three days post-injection. No significant mortalities were observed which is probably related to the non-exposure of adults to anti-nutritional factors that are usually regulated by laccase. Further research should focus either on the feeding larval stage which is directly exposed to anti-nutritional factors, or on other target genes to induce dead phenotypes. This study is the first gene silencing report on a bruchid species and supports RNAi as a potential future method of control.

Cowpea seed beetle, Callosobruchus maculatus (F.) (Coleoptera: Bruchidae), is considered an important bruchid pest in cowpea seed storages. The used pesticides against this pest have caused the occurrence of resistant populations and direct toxicity to the users. The objective of this study was to evaluate the mortality effects of six entomopathogenic fungi isolates obtained from ARSEF (USA) collection [Paecilomyces farinosus (2538), Isaria fumosorosea (4501), Isaria farinosa (3580), Beauveria bassiana (4984), Lecanicillium muscarium (972) and Lecanicillium muscarium (5128)] against C. maculatus adults under laboratory conditions (26±2 °C, 70±5% RH and 16h light: 8h dark). The isolates were cultivated in Potato Dextrose Agar (PDA, Oxoid, CM0139) medium at 26±2 °C in dark conditions for two weeks before using them as control agents. Spore suspensions of the isolates were prepared at two different concentrations (1x105 and 1x107) and mixed with Tween 20 (0.04%). Each concentration was replicated three times and the mortality rates were observed on the 2nd, 4th, 6th, 8th and 10th day of incubations. As a commercial control, a Mycotal extraction of L. muscarium and as a negative control, Tween 20+sterile water was used. Six entomopathogenic fungal isolates at both conidial concentrations yielded high mortalities (from 62.6% to 100%) of C. maculatus adults. These results illustrated that tested fungi strains led to significant mortalities on C. maculatus adults in all the treatments as compared to the controls. Consequently, these fungi strains were regarded as an encouraging alternative method to control the population of C. maculatus adults in the stored cowpea grains. [ABSTRACT FROM AUTHOR]

A study was conducted to know the influence of physical and biochemical parameters on development of the pulse bruchid in green gram at RARS, Lam during 2017-18. The physical parameters of seed viz., colour, shape, surface texture, seed length and width, seed coat hardness and 100 seed weight and biochemical parameters such as protein content, phenol content and total sugars were evaluated for twelve genotypes of greengram. The greengram genotypes with smooth texture, oblong or globose shape and light coloured seed might be less preferred by the pulse bruchid for egg laying. The data showed that genotypes having low sugar and protein contents and high phenol content were resistant to pulse bruchid. The correlation studies showed that biological parameters i.e., number of eggs, adult emergence and growth index had significant positive association with protein content, sugar content, moisture content and electrical conductivity and negative correlation phenol content, 100 seed weight and seed coat hardness. In contrast, mean development period had negative association with protein content, sugar content, electrical conductivity and moisture content and positive correlation with phenol content, 100 seed weight and seed coat hardness. Multiple linear regression studies revealed that all the physical and biochemical properties of seed together were contributing to a large and significant variation (65 to 87 %) in growth parameters of pulse bruchid. [ABSTRACT FROM AUTHOR]

In order to develop a strategy for the safe management of insect pests in stored agricultural commodities, the essential oil of Artemisia herba alba Asso. was tested against Callosobruchus maculatus Fabricius raised on seeds of Cicer arietinum (L.) at 20-30°C and 65±5% relative humidity under storage conditions. Chickpea seeds were fumigated with concentrations of essential oil and then infested by young adults of bruchids. On each treated or control lot, 10 newly emerged pairs were released. The number of dead bruchids and number of eggs hatched and unhatched from the seeds were counted. At the end of their development, adults emerged from treated and untreated seeds were counted separately by sex and the success rate was calculated. Three replicates were conducted for each treatment. The essential oil extracted from Artemisia herba alba Asso was analyzed by gas chromatography coupled with mass spectrometry (GC/MS). The chromatogram of the essential oil of Artemisia herba alba Asso shows 15 signals relating to the 15 compounds of which camphor is the major constituent with a percentage of 96.15%. The results of the biological tests obtained showed that the essential oil of Artemisia herba alba Asso exerts repellent and toxic effects on Callosobruchus maculatus. It causes a high mortality of adults and affects its fertility, fertility, and success rate in a very significant way compared to controls. These show that fumigation of stored commodities with the essential oil of Artemisia herba alba Asso against insect pests can be considered under storage conditions without risk to consumers and the environment. [ABSTRACT FROM AUTHOR]

A study of the morphological characters of the genus Bruchus with taxonomic value is carried out, and the degree of coincidence of these characters between the species of each of the subgeneric taxonomic groups of the genus Bruchus is analyzed, noting that these taxonomic groups present excepcional morphological characters that are not shared by some of the species of each group, except for the characters of the male genitalia. [ABSTRACT FROM AUTHOR]

The data presented in this study provide some basic information regarding seasonal abundance and population fluctuation, the influence of temperature and relative humidity, monthly population and emergence percentages, time and peacks number, and emergence ratios of population density of each leucaena pods and seeds beetle, Acanthoscelides macrophthalmus and its accompaniment parasitoids (Dinarmus basalis Rondani and Lyrcus sp.) emerged from infested Leucaena leucocephala trees. Insects are abundant throughout the year. The highest monthly population of beetles recorded during November, followed by May and December, the highest population of parasitoids observed during February, followed by March and January (D. basalis) also January and August (Lyrcus sp.). Nine peaks for beetles are recoded, the higher peak observed at 4th week Nov. followed by 3rd week of May, then 4th week of January, the same number of peaks recorded for D. basalis parasitoid, the highest peak noticed at 3rd week of Jan. and March, whereas Lyrcus sp. parasitoid has 8 peaks, the higher found at 3rd week of Jan. General ratio of insect's emergence was 1:0.384:0.047 (beetles: Dinarmus: lyrcus). Highest ratios between beetles: parasitoids noticed during February (1:2), July (1:1.774) and March. (1:1.287). The dominant weather factors (MaxTemp., Min. Temp. & RH%.) evidently showed effective changes in the population density of beetles and parasitoids during annual activity months. The correlation between parasitoids and beetles population differed during the different seasons. The current work shows that Dinarmus basalis (26.8% seasonal emergence) and Lyrcus sp. (3.3% seasonal emergence) parasitoids develop within infested leucaena seeds with A. macrophthalmus beetle. Numerous numbers of each beetle (69.9% seasonal emergence) and parasitoids (31.3% seasonal emergence) were observed throughout the year, therefore A. macrophthalmus beetles can be used as a substitute host for mass rearing of D. basalisparasitoids, which are biological agent to control stored seed bruchids. [ABSTRACT FROM AUTHOR]

A find of 2572 charred seeds of pea (Pisum sativum L.) was detected at the Late Bronze Age tell settlement Hissar near Leskovac, in Serbia, belonging to the Brnjica cultural group, 14-10 cent. BC. Two types of pea seeds were observed: apparently healthy seeds and seeds damaged by the activity of a weevil (Coleoptera, Bruchidae). At least twofifths of all finds have apparently been infested most probably by pea weevil (Bruchus pisorum L.), one of the most important pea pests worldwide, especially in medium-moist and dry climates, such as Southern Europe and Australia. A large amount of infested pea seeds indicates a developed pea production on small plots, strongly indicating that cultivating this ancient pulse crop must have been well-rooted in field conditions. Previous DNA analyses of charred pea placed the ancient Hissar pea at an intermediate position between extantly cultivated pea (P. sativum L. subsp. sativum var. sativum) and a wild, winter hardy, 'tall' pea (P. sativum subsp. elatius (Steven ex M. Bieb.) Asch. et Graebn.). Based on an assumption of its late harvest time and combined with pea weevil life cycle stage in charred seeds, it was possible to estimate the season during which the seeds were carbonized, namely, the second half of July or the first days of August at the latest. Older, final weevil instars were predominant before seed carbonization. The pea infestation rate at Hissar is one of the highest noted among pulses in the Old World and the highest among peas, so far. [ABSTRACT FROM AUTHOR]

In this note original data on the Bruchids of the Aspromonte National Park are reported. The specimens examined were surprisingly found within the field activities for a project aimed to deepen the saproxylic coleopteran fauna of the Aspromonte National Park (Calabrian Apennines, Italy), in three different agroforestry systems dominated by chestnut. Out of a total of 134 specimens 129 have been identified as belonging to 3 genera and 14 species, namely: Paleoachantoscelides gilvus (Gyllenhal, 1839), Bruchidius dispar (Gyllenhal, 1833), Bruchidius murinus (Boheman, 1829), Bruchidius olivaceus (Germar, 1824), Bruchidius seminarius (Linnaeus, 1767), Bruchidius tibialis (Boheman, 1829), Bruchidius varius (Olivier, 1795), Bruchidius imbricornis (Panzer, 1795), Bruchidius pygmaeus (Boheman, 1833), Bruchidius trifolii (Motschulsky, 1873), Bruchus brachialis Fahraeus, 1839, Bruchus pisorum (Linnaeus, 1758), Bruchus rufipes Herbst, 1783, Bruchus viciae. [ABSTRACT FROM AUTHOR]

There is a common perception that stored-product pests cause high losses only in developing countries. This may not be true because of the import of infested commodities in freight containers, especially after the ban of the broad-spectrum fumigant methyl bromide. However, infestation quantification of such infested imports has been rare in Europe because, in addition to other factors, stored-product pests have lost their quarantine status. This work documented that heavily infested commodities may be transported to Europe from a different continent (i.e., East Africa). In particular, we present the first study to quantify the entire legume-infesting pest population that may be transported in a single freight container. The quantification of the extent of adult pest infestation was performed not by taking a limited number of samples but by sieving the content of the entire container. From the analysed freight container loaded with 24 tons of infested pinto beans, 1,101,060 adult individuals of the Mexican bean weevil, Zabrotes subfasciatus (Bohemann) (Coleoptera Chrysomelidae), were extracted. This represents a density of 45.9 adults per 1 kg of imported beans. Such a huge amount of beetles per freight container holding bean commodity is 40x more than that predicted by the theoretical estimates. The visible damage to the commodity (i.e., bean kernels with physical injury and loaded with eggs) was also profound, reaching 10%; it represents 901 440 damaged kernels per container loaded with 24 t of beans. Our findings indicate that even a single freight container transporting commodities from different continents to Europe may host pest populations exceeding one million invasive pest specimens. This may have significant importance not only in terms of the hidden contamination of human food by internally feeding and allergenic pests but also in regard to the risk of spread of entire populations and different biotypes (e.g., the transfer of genes for insecticide resistance). [ABSTRACT FROM AUTHOR]

En este trabajo se describen la morfología de las primeras larvas maduras y los ciclos de vida de Amblycerus hoffmanseggi (Gyll.) (Amblycerinae), Acanthoscelides comptus Kingsolver y Merobru chus bicoloripes (Pic) (Bruchinae), además, el genital femenino de A. hoffmanseggi y se incluye la redescripción de M. bicoloripes.

Se describen aquí los caracteres bionómicos y morfológicos de las etapas preimaginales de Amblycerus caryoboriformis (Pic), Sennius laminifer (Sharp) y Stator bisbimaculatus (Pic), tres especies de Bruchidae comúnmente encontradas en el noroeste de Argentina. También se da la morfología de los adultos de A. caryoboriformis y S. bisbimaculatus. Se comparan los resultados con los ya conocidos de otras especies del mismo género y con otros grupos de la familia mencionada.

Se investigaron los cromosomas meióticos de 20 machos de Scutobruchus cassiovorus y 20 machos de Merobruchus bicoloripes para determinar su constitución cromosómica. Los cariotipos encontrados fueron: 2n = 18, n = 8Xyp para Scutobruchus cessiovorus y 2n = 24, n = 11Xyp, para Merobruchus bicoloripes. Se analizan las diferencias numéricas y estructurales entre ellos y otras especies de Bruchinae.

En este artículo se describe la morfología de la primera y madura larva y el ciclo de vida de Amblycerus longesuturalis (Pic). También se proporcionan nuevos estudios de morfología del adulto.

Callosobruchus chinensis causes damage to the Phaseolus vulgaris seeds. Traditionally, Dioscorea sansibarensis serves as a medicinal plant. Naturally, D. sansibarensis has toxins that protect against herbivores and the surrounding invasive plants in its natural habitat. Phytochemical analysis by thin-layer chromatography (TLC) and laboratory experiments was carried out to determine the activity of D. sansibarensis leaves, bulbils, and yams powders on antioviposition and inhibition of the F1 emergence of C. chinensis. Bioassay data were subjected to nonparametric (Kolmogorov–Smirnov) statistical analysis and a generalized linear model at P ≤ 0.05. Statistically, the powders had an antioviposition activity of 34.3% (R2 = 0.343). A recommendable activity on antioviposition was displayed by the yams powder; treatment by 0.8 g of yams powder had a Wald Chi-Square value of 1.291, P = 0.26. Inhibition of F1 emergence was significantly attained by the yams powder; the treatment by 0.6 g of yams powder had a Wald Chi-Square value of 7.72, P = 0.01. Statistically, the bulbils powder displayed low antioviposition and inhibition of F1 emergence. Observations on the TLC exposed compounds with similar Rf values; saponin with an Rf value of 0.72 was portrayed in the leaves, bulbils, and yams. A terpenoid and a flavonoid with Rf values of 0.37 and 0.71, respectively, were observed in bulbils and yams but absent in leaves. A terpenoid with an Rf value of 0.49 was visualized in leaves and bulbils but not in the yams powder. The study concluded that the D. sansibarensis yams and leaves powders are viable for application by the farmers in the protection of stored legumes against attack by C. chinensis. However, there may be other diverse interests in other storage insects and other methods of phytochemical analysis that have not been investigated. [ABSTRACT FROM AUTHOR]

Background: Fumes from high-temperature heated plant leaves containing volatile phytochemicals generated from Lantana camara, Cinnamomum zeylanicum, Azadirachta indica and Ocimum sanctum were tested for their insecticidal activity against adult southern cowpea weevil (Callosobruchus chinensis L.) and their F1 progeny production/emergence. Volatile phytochemicals containing fume was generated using a flameless dry heat extraction method similar to pyrolysis combustion without air supplement at 180 ± 5 °C. Insect mortalities were assessed up to 72 h after exposure to the different treatments of fumigation by plant-fume and control. Results: All volatile plant-fume samples contained average of 16.3 ± 1.5% O2 and 5.8 ± 0.5% CO2 in the test jars. The F1 progeny emergence was estimated 30 days after treatment. After 36 h of exposure, L. camara showed the highest toxicity against C. chinensis, followed by O. sanctum, A. indica and C. zeylanicum, with LT50 values of 7.3, 9.4, 14.7 and 20.6 h, respectively. The volatile phytochemical containing plant-fume generated by A. indica and C. zeylanicum produced LT99 values that were not significantly different (P > 0.05) from each other. The F1 adult emergence from treated mungbean (Vigna radiata L.) samples was significantly inhibited by L. camara and A. indica volatile plant-fume compared to C. zeylanicum fume. However, plant-fume generated from all four plants exhibited effective direct toxicity and F1 progeny inhibition of more than 86%. Conclusion: From the study, it can be concluded that volatile plant-fume treatment was highly lethal to C. chinensis and significantly reduced F1 progeny emergence. Therefore, phytochemicals obtained from thermal extraction technique can be used as an alternative technique to chemical fumigation of stored mungbean. [ABSTRACT FROM AUTHOR]

Copyright of Revista Mexicana de Biodiversidad is the property of Universidad Nacional Autonoma de Mexico, Instituto de Biologia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

A preliminary catalog of seeds beetles (Coleoptera: Bruchidae) from the Autonomous Community of Madrid (Spain) is presented. After the study of about 300 specimens, mostly from the National Museum of Natural Sciences of Madrid, a total of 41 species of seed beetles were obtained, included into six genera, of which the most numerous were the genus Bruchidius (with 21 species) and the genus Bruchus (with 13 species), representing approximately 45,2 % of the Iberian-Balearic fauna of this group of Coleoptera. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Plant Protection & Pathology is the property of Egyptian National Agricultural Library (ENAL) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Arab Journal of Plant Protection is the property of Arab Society for Plant Protection and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The present study aimed to investigate new insecticidal compounds used as environmentally safe alternative to synthetic insecticides. A laboratory study was conducted to investigate the effects of Bacillus biofilms and their extracellular matrix (ECM) on the adult's mortality and reproduction of the cowpea weevil, Callosobruchus maculatus (F.) (Coleoptera: Bruchidae). Among 30 bacterial isolates, only 4 exhibited high abilities to form biofilm and showed potentials against the pest at different concentrations 5, 10 and 20%. Results showed that the mortality percentage of C. maculatus adults ranged between 13.36 and 63.43% overall and it increased significantly with raising the concentration of ECMs. While the latent larval mortality did not exceed 31.42%, which was recorded with ECM of isolate no. 13 at the highest concentration (20%). Further, all tested concentrations reduced female fecundity and egg hatchability. The most potent biofilm-producing isolate having the highest adverse activity of ECM extract was identified as Bacillus flexus S13, using 16S rDNA gene sequence and submitted in GenBank under accession number MK292147. These results demonstrated that B. flexus S13 biofilm is a good strategy for the development of new biocontrol agents against C. maculatus. [ABSTRACT FROM AUTHOR]

This study was conducted to screen the insecticidal activity of five plant essential oils, subsequently evaluated the seed treatment effect on mung bean seed with the selected plant oil against the pulse beetle, Callosobruchus maculatus, under laboratory conditions. Residual contact was conducted to screen insecticidal activity. Insect mortality and LC50 were performed after 24, 48 and 72 h of treatment. Clove oil was the most toxic to C. maculatus with the lowest LC50 values of 16.09, 12.99 and 7.67 μl/ml and achieved the highest mortality after 24, 48 and 72 h of exposure, respectively. Toxicity of plant oils was categorized in a descending order of clove>cinnamon>lengkuas>citronella>kaffir lime. Clove oil was selected to investigate for seed treatment on mung bean seeds to observe the bioactivity of plant oil. In seed dressing application, bioactivity of clove oil was evaluated with five different concentrations of 0.4, 0.8, 1.2, 1.6 and 2 ml/kg on mung bean seeds. Results show 100% mortality of C. maculatus adults at the concentration of 2 ml/kg seeds after 5 days of exposure, and mortality percentage of 22.50 ± 4.79 at the lowest concentration level of 0.4 ml/kg seeds after the same exposure time. Furthermore, the five concentrations of clove oil significantly reduced F1 progeny, seed damage, weight loss of mung bean, and increased the inhibition rate. Findings of this study suggest that clove oil can be used as an alternative to synthetic insecticides for integrated pest management against insect pests, especially C. maculatus, on mung bean seeds under storage conditions. [ABSTRACT FROM AUTHOR]

A study is carried out on the reliability of the morphological characters attributed to Pseudopachymerina grata (Terán, 1990) for its differentiation from Pseudopachymerina spinipes (Erichson, 1833) species concluding that, given the great variability of characters such as the female pigidian spot pattern or the number of denticles of the pecten of the metafemur, these characters are not valid to differentiate both species. The male's genitalia is the only character that allows the two species to be safely differentiated. [ABSTRACT FROM AUTHOR]

From the available bibliographic data, as well as the inventories of species of various collections, all of them collected in the database of BRUCOL, of the Postgraduate College, México, an annotated checklist of species of seed beetles (Coleoptera, Bruchidae) from Bolivia has been prepared. As a result, a list of 95 species of seed beetles is showed, of which 21 species are new records to the currently known Bolivian fauna. Collaterally, this study also detected 23 new records for various countries in America. Moreover, distribution data is offered, which shows the existence of five potentially endemic species of Bolivia and a fauna close to the Brazilian. Finally, a list of host plants of each species is offered, obtaining 11 new host records for some species; in general, the bruchid species presented a predominantly monophago-oligophage trophic regimen in its different grades. [ABSTRACT FROM AUTHOR]