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Bu çalışmada Sort1 gen ekspresyonunun lipoprotein altsınıfları ve KAH ile ilişkisini incelendi. Ayrıca Sort1 ile aynı lokusta yer alan ve genin ekspresyonunu etkileyen rs599839 polimorfizmi çalışıldı. Çalışma grupları; anjiyografi sonucu çeşitli düzeylerde damar tıkanıklığı tespit edilen 162 hasta ve 49 sağlıklı bireylerden oluşturulmuştur. Çalışma gruplarında lipit profilleri (TAG, TK, LDL, HDL), serum apolipoprotein (ApoA, ApoB ve ApoE) seviyeleri ve CRP değeri otoanalizörde çalışıldı. Lipoproten altsınıfları Qantimetrix lipoprint sistem kullanılarak analiz edildi. Sort1 gen ekspresyonu düzeyi ise Human Sortilin TaqMan Gene Expression Assays kullanılarak qRT-PCR metoduyla ile belirlendi. Genomik DNA tam kandan izole edildi ve rs599839 polimorfiziminin belirlenmesi için genotipleme Polimeraz Zincir Reaksiyonu-Restiriksiyon Fragment Uzunluk Polimorfizmi (PCR-RFLP) metadu ile çalışıldı. Kontrol grubuna göre KAH grubunda Sort1 gen ekspresyonu anlamlı derecede artmıştır (p0,05). Çalışma grubunda lipoprotein altsınıfları karşılaştırıldığında; kontrol grubuna göre KAH'da aterojenik altfraksiyonlar (SdLDL-K ve SHDL) istatistiksel olarak daha yüksek bulunmuştur (p 0,05) were identified in Sort1 gene expression between variant allele groups of rs599839. When the lipoprotein subclasses were evaluated in the study groups; atherogenic subfractions (SdLDL-K and SHDL) were found to be statistically higher in CAD than control group (p

yilmaz, adnan/0000-0003-4842-1173; duman, hakan/0000-0002-1441-7320 WOS: 000357755800011 PubMed: 26226707 Objective High-density lipoprotein cholesterol (HDL-C) levels are inversely related to the risk of coronary artery disease (CAD). Alterations in HDL-C subclass distribution and HDL-associated enzyme activities may be more important than total HDL levels for the progression of CAD. We intended to investigate the relationship of HDL-C subclass distribution and HDL-associated enzyme activities with CAD. Method and results Our study included 101 patients with stable coronary artery disease, and 64 healthy subjects. Serum levels of HDL lipoprotein-associated-phospholipase A(2) (HDL-LpPLA(2)), paraoxonase 1 (PON1), and HDL subfraction distribution were measured. We found increased small HDL (sHDL) subfractions in patients with one-vessel disease (P < 0.001).We also found a reverse correlation between total HDL-C levels and affected vessel number (P

Mercantepe, Tolga/0000-0002-8506-1755; Dokumacioglu, Eda/0000-0002-2223-1331 WOS: 000461449400024 PubMed: 30798139 Objective: Nephrotoxicity is the most important side effect of the antineoplastic drug cisplatin, thereby restricting its use. the aim of this study was to investigate the protective effects of white tea infusions(WT) against renal damage induced by cisplatin(CP) in rats by biochemical and histopathological means. Materials and methods: This study used 24 female Sprague Dawley rats at 12-14 weeks of age and weighing 250-300 g. Rats were divided into three groups: Control, CP and CP+ WT groups. CP was injected 7 mg/kg i.p as a single dose/rat in the CP group. White tea was given at a dose of 0.5%(w/v) for 4 weeks. At the end of the experiment, blood urea nitrogen(BUN), creatinine, uric acid, tumor necrosis factor-alpha(TNF-alpha), interleukin-6 (IL-6) and nuclear factor kappa B(NF-kappa B) along with caspase-3 in the kidney were evaluated in study. Results: BUN, creatinine, TNF-alpha, NF-kappa B and IL-6 levels of the CP group showed a statisically significant increase in comparison to the control group. TNF-alpha, NF-kappa B and IL-6 levels showed a statistically significant decrease in the CP+ WT group with respect to the CP group. Caspase-3 levels in tubular epithelial cells decreased in CP+ WT group compared with CP group(p = 0.02). Conclusion: White tea infusions reduced significantly the nephrotoxicity of CP. the anti-nephrotoxic feature of the infusion may be attributed primarily to its anti-inflammatory and anti-apoptotic characteristics. scientific research foundation of Recep Tayyip Erdogan University The study was funded by a grant from the scientific research foundation of Recep Tayyip Erdogan University.

To analyze the phenolic composition of the Cimin grape, which is 1 of 2 grape varieties with a protected geographical indication in Turkey and is used locally to treat the symptoms of some disorders such as impotence and cardiovascular diseases, and to investigate its antioxidant potency against oxidant mediators in the models of intra- and extracellular surroundings. Materials and methods: Cimin grape samples were classified into 5 groups according to the grape's tissues and the extraction solvents used. Free radical scavenging (DPPH) and antilipid peroxidation product [thiobarbituric acid reactive substance (TBARS) and conjugated diene (CD)] levels of the grape tissues were extrapolated from the measurement of total phenolic and individual monomeric flavanol contents in each extract. Results: The seed methanolic extract had the highest total amount of flavanols, with the amount of catechin at 4.034 mM. The DPPH activities of the seed extracts were 2- to 10-fold higher than those of the other samples. The seed extract-treated erythrocyte and unfractionated human plasma also showed lower TBARS and CD values. In addition, regeneration of glutathione was more obvious in grape seed extracts than in the rest of the tissues. Conclusion: The underlying mechanism of these changes can be related mainly to increased antioxidant status. Cimin grape consumption may have beneficial effects on health maintenance.

yilmaz, adnan/0000-0003-4842-1173 WOS: 000330341200002 PubMed: 24240543 Atherogenic dyslipidemia characterized by abnormal changes in plasma lipid profile such as low high-density lipoprotein (HDL) and increased triglyceride (TG) levels is strongly associated with atherosclerotic diseases. We aimed to evaluate the levels of pro- and antiatherogenic lipids and erythrocyte membrane cholesterol (EMC) content in normo- and dyslipidemic subjects to investigate whether EMC content could be a useful marker for clinical presentation of atherogenic dyslipidemia. Low-density lipoprotein (LDL), HDL and their subfraction levels and erythrocyte lipid content were determined in 64 normolipidemic (NLs), 42 hypercholesterolemic (HCs) and 42 mixed-type dyslipidemic subjects (MTDs). Plasma atherogenic lipid indices [small-dense LDL (sdLDL)/less-dense HDL (LHDL), TC/HDL-C, TG/HDL-C and Apo B/AI] were higher in MTDs compared to NLs (p < 0.001). the highest sdLDL level was observed in HCs (p < 0.01). Despite a slight increase in EMC level in dyslipidemic subgroups, the difference was not statistically significant. A significant negative correlation, however, was observed between EMC and sdLDL/LHDL in HCs (p < 0.035, r = -0.386). Receiver operating characteristic curves to predict sdLDL level showed that TG and EMC levels had higher area under curve values compared to other parameters in HCs. We showed that diameters of larger LDL and HDL particles tend to shift toward smaller values in MTDs. Our results suggest that EMC content and TG levels may be a useful predictor for sdLDL level in hypercholesterolemic patients. TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [2008.102.02.2] Supported in part by TUBITAK (Grant No. 2008.102.02.2).