538 results on '"Ao, Lin"'
Search Results
2. Rhythms of Victory: Predicting Professional Tennis Matches Using Machine Learning
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Yilin Lei, Ao Lin, and Jianuo Cao
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Forecasting ,support vector machine ,machine learning ,particle swarm optimization ,tennis ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 - Abstract
Forecasting the winning matches of professional tennis players has a wide range of practical applications. We introduced an innovative approach to quantify and combine strategic and psychological momentum using the entropy weight method and analytic hierarchy process, and tested its effectiveness. Utilizing data from the Wimbledon Championship 2023, we constructed a support vector machine model to predict the turning point and winner of each point, and optimized it using particle swarm optimization. Our model achieved a significant level of accuracy (96.09% for turning point and 83.52% for predicting the winner) and performed well in different courts and players. Furthermore, we compared its performance with commonly utilized predictive models, including ARIMA, LSTM and BP networks, and found that our model exhibited higher accuracy than other existing models in predicting the point winner. Our study provides a reference for the role of momentum in dynamic matches, and our model can be used to calculate the odds of tennis matches and provide guidance to coaches.
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- 2024
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3. Robust positive association between serum urate and the risk of chronic obstructive pulmonary disease: hospital-based cohort and Mendelian randomisation study
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Xinhua Wang, Dongsheng Huang, Jiachun Lu, Boqi Rao, Dongming Xie, Yibin Deng, Junyi Ye, Xiaobin Zeng, Ao Lin, Jinbin Chen, Chenli Xie, Cuiyi Chen, Yixuan Luo, and Xiaoxiao Lu
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Medicine ,Diseases of the respiratory system ,RC705-779 - Abstract
Background Chronic obstructive pulmonary disease (COPD) and hyperuricaemia are both characterised by systemic inflammation. Preventing chronic diseases among the population with common metabolic abnormality is an effective strategy. However, the association of hyperuricaemia with the higher incidence and risk of COPD remains controversial. Therefore, replicated researches in populations with distinct characteristics or demographics are compellingly warranted.Methods This cohort study adopted a design of ambispective hospital-based cohort. We used propensity score matching (PSM) and inverse probability of treatment weighting (IPTW) to minimise the effects of potential confounding factors. A Cox regression model and restricted cubic spline (RCS) model were applied further to assess the effect of serum urate on the risk of developing COPD. Finally, we conducted a two-sample Mendelian randomisation (MR) analysis to explore evidence of causal association.Results There is a higher incidence in the population with hyperuricaemia compared with the population with normal serum urate (22.29/1000 person-years vs 8.89/1000 person-years, p=0.009). This result is robust after performing PSM (p=0.013) and IPTW (p
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- 2024
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4. Polystyrene nanoplastics exposure triggers spermatogenic cell senescence via the Sirt1/ROS axis
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Liang, Yuehui, Yang, Yurui, Lu, Chunsheng, Cheng, Ya, Jiang, Xiao, Yang, Binwei, Li, Yawen, Chen, Qing, Ao, Lin, Cao, Jia, Han, Fei, Liu, Jinyi, and Zhao, Lina
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- 2024
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5. Perturbation of IP3R-dependent endoplasmic reticulum calcium homeostasis by PPARδ-activated metabolic stress leads to mouse spermatocyte apoptosis: A direct mechanism for perfluorooctane sulfonic acid-induced spermatogenic disorders
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Yang, Wang, Ling, Xi, He, Shijun, Cui, Haonan, Wang, Lihong, Yang, Zeyu, An, Huihui, Zou, Peng, Chen, Qing, Sun, Lei, Yang, Huan, Liu, Jinyi, Cao, Jia, and Ao, Lin
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- 2024
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6. The role of EPAS1 polymorphisms on COPD susceptibility in southern Chinese
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Yunchao Wang, Ao Lin, Ruiqi He, Cuiyi Chen, Xiaobin Zeng, Yujie Pan, Chun Mao, Chenli Xie, Dongsheng Huang, Yibin Deng, Xuhui Zhang, Jiachun Lu, and Xinhua Wang
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COPD ,EPAS1 ,Polymorphism ,Susceptibility ,Absolute risk ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Objective: COPD is the most common chronic respiratory disease with complex environmental and genetic etiologies. It was reported that EPAS1 might participate in the occurrence and development of respiratory diseases. However, the association between EPAS1 and COPD was unclear. Methods: First, a case-control study enrolling 1130 COPD patients and 1115 healthy controls in Guangzhou was conducted to clarify the association between EPAS1 polymorphisms and COPD susceptibility. Secondly, a prevalence study recruited 882 participants in Gansu to verify the effect of positive polymorphisms on lung function. Finally, the 10-year absolute risk considering environmental factors and genetic variations was calculated by the method of Gail and Bruzzi. Results: EPAS1 rs13419896 AA genotype reduced COPD risk in southern Chinese (AA vs. GG: adjusted OR = 0.689, 95% CI = 0.498–0.955; AA vs. GG/GA: adjusted OR = 0.701, 95% CI = 0.511–0.962). Further, the rs13419896 A allele was significantly associated with higher pre-FEV1/pre-FVC in both the Guangzhou and Gansu populations (P A were finally retained to develop a relative risk model for males. Smoking status, biomass as fuels, and rs13419896 G > A were retained in the female model. The population-attributable risk of the male or female model was 0.457 (0.283–0.632) and 0.421 (0.227–0.616), respectively. Conclusions: This study first revealed that EPAS1 rs13419896 G > A decreased COPD susceptibility and could be a genetic marker to predict the 10-year absolute risk for COPD.
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- 2023
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7. Activation of eIF4E‐binding‐protein‐1 rescues mTORC1‐induced sarcopenia by expanding lysosomal degradation capacity
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Elisa M. Crombie, Seonyoung Kim, Stuart Adamson, Han Dong, Tzu‐Chiao Lu, Yiju Wu, Yajun Wu, Yotam Levy, Nolan Stimple, Wing Moon R. Lam, Hwee Weng D. Hey, Dominic J. Withers, Ao‐Lin Hsu, Boon Huat Bay, Julien Ochala, and Shih‐Yin Tsai
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mitochondrial dysfunction ,mRNA translation ,mTORC1 ,protein degradation ,sarcopenia ,Diseases of the musculoskeletal system ,RC925-935 ,Human anatomy ,QM1-695 - Abstract
Abstract Background Chronic mTORC1 activation in skeletal muscle is linked with age‐associated loss of muscle mass and strength, known as sarcopenia. Genetic activation of mTORC1 by conditionally ablating mTORC1 upstream inhibitor TSC1 in skeletal muscle accelerates sarcopenia development in adult mice. Conversely, genetic suppression of mTORC1 downstream effectors of protein synthesis delays sarcopenia in natural aging mice. mTORC1 promotes protein synthesis by activating ribosomal protein S6 kinases (S6Ks) and inhibiting eIF4E‐binding proteins (4EBPs). Whole‐body knockout of S6K1 or muscle‐specific over‐expression of a 4EBP1 mutant transgene (4EBP1mt), which is resistant to mTORC1‐mediated inhibition, ameliorates muscle loss with age and preserves muscle function by enhancing mitochondria activities, despite both transgenic mice showing retarded muscle growth at a young age. Why repression of mTORC1‐mediated protein synthesis can mitigate progressive muscle atrophy and dysfunction with age remains unclear. Methods Mice with myofiber‐specific knockout of TSC1 (TSC1mKO), in which mTORC1 is hyperactivated in fully differentiated myofibers, were used as a mouse model of sarcopenia. To elucidate the role of mTORC1‐mediated protein synthesis in regulating muscle mass and physiology, we bred the 4EBP1mt transgene or S6k1 floxed mice into the TSC1mKO mouse background to generate 4EBP1mt‐TSC1mKO or S6K1‐TSC1mKO mice, respectively. Functional and molecular analyses were performed to assess their role in sarcopenia development. Results Here, we show that 4EBP1mt‐TSC1mKO, but not S6K1‐TSC1mKO, preserved muscle mass (36.7% increase compared with TSC1mKO, P
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- 2023
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8. PPARα/ACOX1 as a novel target for hepatic lipid metabolism disorders induced by per- and polyfluoroalkyl substances: An integrated approach
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Yang, Wang, Ling, Xi, He, Shijun, Cui, Haonan, Yang, Zeyu, An, Huihui, Wang, Lihong, Zou, Peng, Chen, Qing, Liu, Jinyi, Ao, Lin, and Cao, Jia
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- 2023
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9. High-throughput small molecule screening reveals Nrf2-dependent and -independent pathways of cellular stress resistance.
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Lombard, David, Kohler, William, Guo, Angela, Gendron, Christi, Han, Melissa, Ding, Weiqiao, Lyu, Yang, Ching, Tsui-Ting, Wang, Feng-Yung, Chakraborty, Tuhin, Nikolovska-Coleska, Zaneta, Duan, Yuzhu, Girke, Thomas, Hsu, Ao-Lin, Pletcher, Scott, and Miller, Richard
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Animals ,Caenorhabditis elegans ,Caenorhabditis elegans Proteins ,DNA-Binding Proteins ,Longevity ,Mammals ,NF-E2-Related Factor 2 - Abstract
Aging is the dominant risk factor for most chronic diseases. Development of antiaging interventions offers the promise of preventing many such illnesses simultaneously. Cellular stress resistance is an evolutionarily conserved feature of longevity. Here, we identify compounds that induced resistance to the superoxide generator paraquat (PQ), the heavy metal cadmium (Cd), and the DNA alkylator methyl methanesulfonate (MMS). Some rescue compounds conferred resistance to a single stressor, while others provoked multiplex resistance. Induction of stress resistance in fibroblasts was predictive of longevity extension in a published large-scale longevity screen in Caenorhabditis elegans, although not in testing performed in worms and flies with a more restricted set of compounds. Transcriptomic analysis and genetic studies implicated Nrf2/SKN-1 signaling in stress resistance provided by two protective compounds, cardamonin and AEG 3482. Small molecules identified in this work may represent attractive tools to elucidate mechanisms of stress resistance in mammalian cells.
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- 2020
10. A Case-Control Study of the Associations between EGLN1 Gene Polymorphisms and COPD
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Xin Li, Peng Zhang, Jing Yu, Chunyan Zhang, Yanli Shi, Xueyan Wei, Honge Li, Xuhui Zhang, Ao Lin, Zhen Yang, Yunchao Wang, and Xinhua Wang
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copd ,egln1 ,polymorphism ,susceptibility ,Biochemistry ,QD415-436 ,Biology (General) ,QH301-705.5 - Abstract
Background: Environmental and genetic factors are jointly involved in the development of chronic obstructive pulmonary disease (COPD). The EGLN1 gene is a major factor in upstream regulation of the hypoxia-inducible pathway. EGLN1 negatively regulates the hypoxia-inducible factors HIF-lα and HIF-2α by regulating the concentration of oxygen, mainly in a hypoxic environment. Hypoxia is a common physiologic condition during the progression of COPD, and several studies have identified genetic variants in EGLN1 as a key factor in the adaptation to hypoxic environments. However, it is still unclear whether there is an association between EGLN1 variants and the risk of developing COPD. Methods: A case-control study was conducted in the Gannan Tibetan Autonomous Prefecture, Gansu Province. A total of 292 COPD patients and 297 healthy controls were enrolled to assess the association of EGLN1 single nucleotide polymorphisms (SNPs) (rs41303095 A>G, rs480902 C>T, rs12097901 C>G, rs2153364 G>A) with COPD susceptibility. Results: The EGLN1 rs41303095 A>G, rs480902 C>T, rs12097901 C>G, and rs2153364 G>A polymorphisms were not associated with COPD susceptibility (p > 0.05). Conclusions: The EGLN1 rs41303095 A>G, rs480902 C>T, rs12097901 C>G and rs2153364 G>A polymorphisms were found in this study not to be associated with susceptibility to COPD in Gannan Tibetans.
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- 2024
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11. A summary index derived from Kinect to evaluate postural abnormalities severity in Parkinson’s Disease patients
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Ronghua Hong, Tianyu Zhang, Zhuoyu Zhang, Zhuang Wu, Ao Lin, Xiaoyun Su, Yue Jin, Yichen Gao, Kangwen Peng, Lixi Li, Lizhen Pan, Hongping Zhi, Qiang Guan, and Lingjing Jin
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Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Postural abnormalities are common disabling motor complications affecting patients with Parkinson’s disease (PD). We proposed a summary index for postural abnormalities (IPA) based on Kinect depth camera and explored the clinical value of this indicator. Seventy individuals with PD and thirty age-matched healthy controls (HCs) were enrolled. All participants were tested using a Kinect-based system with IPA automatically obtained by algorithms. Significant correlations were detected between IPA and the Movement Disorder Society-Sponsored Revision of the Unified Parkinson’s Disease Rating Scale (MDS-UPDRS) total score (r s = 0.369, p = 0.002), MDS-UPDRS-III total score (r s = 0.431, p
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- 2022
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12. Development of a traditional Chinese medicine‐based agent for the treatment of cancer cachexia
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Kun‐Chang Wu, Po‐Chen Chu, Yu‐Jung Cheng, Chia‐Ing Li, Jingkui Tian, Hsing‐Yu Wu, Szu‐Hsien Wu, Yi‐Chun Lai, Hsiang‐Han Kao, Ao‐Lin Hsu, Hsiang‐Wen Lin, and Chih‐Hsueh Lin
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Cachexia ,Traditional Chinese medicine ,Muscle atrophy ,C2C12 cell model ,C‐26 tumour‐bearing model ,Caenorhabditis elegans model ,Diseases of the musculoskeletal system ,RC925-935 ,Human anatomy ,QM1-695 - Abstract
Abstract Background Despite recent advances in understanding the pathophysiology of cancer cachexia, prevention/treatment of this debilitating disease remains an unmet medical need. Methods We developed an integrated, multi‐tiered strategy involving both in vitro and in vivo muscle atrophy platforms to identify traditional Chinese medicine (TCM)‐based anti‐cachectic agents. In the initial screening, we used inflammatory cytokine‐induced atrophy of C2C12 myotubes as a phenotypic screening platform to assess the protective effects of TCMs. The selected TCMs were then evaluated for their abilities to protect Caenorhabditis elegans from age‐related reduction of mobility and contractility, followed by the C‐26 colon adenocarcinoma mouse model of cachexia to confirm the anti‐muscle atrophy effects (body/skeletal muscle weights, fibre size distribution, grip strengths, and serum IL‐6). Transcriptome analysis, quantitative real‐time polymerase chain reaction, and immunoblotting were performed to gain understanding of the potential mechanism(s) by which effective TCM protected against C26 tumour‐induced muscle atrophy. Results Of 29 widely used TCMs, Dioscorea radix (DR) and Mu Dan Pi (MDP) showed a complete protection (all P values, 0.0002) vis‐à‐vis C26 conditioned medium control in the myotube atrophy platform. MDP exhibited a unique ability to ameliorate age‐associated decreases in worm mobility, accompanied by improved total body contractions, relative to control (P
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- 2022
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13. Development and validation of nomogram including high altitude as a risk factor for COPD: A cross-sectional study based on Gansu population
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Ao Lin, Chun Mao, Boqi Rao, Hongjun Zhao, Yunchao Wang, Guokang Yang, Haisheng Lei, Chenli Xie, Dongsheng Huang, Yibin Deng, Xuhui Zhang, Xinhua Wang, and Jiachun Lu
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COPD ,altitude ,prevalence ,risk factor ,nomogram ,Public aspects of medicine ,RA1-1270 - Abstract
BackgroundChronic Obstructive Pulmonary Disease (COPD) is a common and harmful disease that requires an effective tool to early screen high-risk individuals. Gansu has unique environments and customs, leading to the different prevalence and etiology of COPD from other regions. The association between altitude and COPD once attracted epidemiologists' attention. However, the prevalence in Gansu and the role of altitude are still unclarified.MethodsIn Gansu, a multistage stratified cluster sampling procedure was utilized to select a representative sample aged 40 years or older. The questionnaire and spirometry examination were implemented to collect participants' information. The diagnosis and assessment of COPD were identified by the Global Initiative for Chronic Obstructive Lung Disease (GOLD) criterion, while post-bronchodilator FEV1/FVC < LLN was for sensitivity analysis. Furthermore, the effect of high altitude on COPD was evaluated by the logistic regression model after propensity score matching (PSM). Finally, the participants were randomly divided into training and validation sets. The training set was used to screen the relative factors and construct a nomogram which was further assessed by the receiver operating characteristic (ROC) curve, calibration curve, and decision curve analysis (DCA) in the two sets.ResultsThere were 2,486 eligible participants in the final analysis, of which 1,584 lived in low altitudes and 902 lived in high altitudes. Based on the GOLD criterion, the crude and standardized prevalences in Gansu were 20.4% (18.7–22.0) and 19.7% (17.9–21.6). After PSM, the logistic regression model indicated that high altitude increased COPD risk [PSM OR: 1.516 (1.162–1.978)]. Altitude, age, sex, history of tuberculosis, coal as fuel, and smoking status were reserved for developing a nomogram that demonstrated excellent discrimination, calibration, and clinical benefit in the two sets.ConclusionsCOPD has become a serious public health problem in Gansu. High altitude is a risk factor for COPD. The nomogram has satisfactory efficiency in screening high-risk individuals.
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- 2023
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14. High clinical diagnostic accuracy of combined salivary gland and myocardial metaiodobenzylguanidine scintigraphy in the diagnosis of Parkinson’s disease
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Shuangfang Li, Lei Yue, Shuzhen Chen, Zhuang Wu, Jingxing Zhang, Ronghua Hong, Ludi Xie, Kangwen Peng, Chenghong Wang, Ao Lin, Lingjing Jin, and Qiang Guan
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Parkinson’s disease ,parkinsonism ,salivary glands ,MIBG scintigraphy ,diagnosis ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
BackgroundDecreased myocardial uptake of 131I-metaiodobenzylguanidine (MIBG) is known to be an important feature to diagnose Parkinson’s disease (PD). However, the diagnosis accuracy of myocardial MIBG scintigraphy alone is often unsatisfying. Recent studies have found that the MIBG uptake of the major salivary glands was reduced in PD patients as well.PurposeTo evaluate the diagnostic value of major salivary gland MIBG scintigraphy in PD, and explore the potential role of myocardial MIBG scintigraphy combined with salivary gland MIBG scintigraphy in distinguishing PD from non-PD (NPD).MethodsThirty-seven subjects were performed with 131I-MIBG scintigraphy. They were classified into the PD group (N = 18) and the NPD group (N = 19), based on clinical diagnostic criteria, DAT PET and 18F-FDG PET imaging findings. Images of salivary glands and myocardium were outlined to calculated the MIBG uptake ratios.ResultsThe combination of left parotid and left submandibular gland early images had a good performance in distinguishing PD from NPD, with sensitivity, specificity, and accuracy of 50.00, 94.74, and 72.37%, respectively. Combining the major salivary gland and myocardial scintigraphy results in the early period showed a good diagnostic value with AUC, sensitivity and specificity of 0.877, 77.78, and 94.74%, respectively. Meanwhile, in the delayed period yield an excellent diagnostic value with AUC, sensitivity and specificity of 0.904, 88.89, and 84.21%, respectively.Conclusion131I-MIBG salivary gland scintigraphy assisted in the diagnosis and differential diagnosis of PD. The combination of major salivary gland and myocardial 131I-MIBG scintigraphy further increased the accuracy of PD diagnosis.
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- 2023
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15. EPO rs1617640 A>C is a Protective Factor for Chronic Obstructive Pulmonary Disease: A Case Control Study
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Yunchao Wang, Zhi Li, Xiaoyi Zhang, Ao Lin, Cuiyi Chen, Xiaobin Zeng, Yujie Pan, Chun Mao, Chenli Xie, Dongsheng Huang, Yibin Deng, Xuhui Zhang, Jiachun Lu, and Xinhua Wang
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copd ,epo ,polymorphism ,susceptibility ,absolute risk ,Biochemistry ,QD415-436 ,Biology (General) ,QH301-705.5 - Abstract
Background: The occurrence and development of chronic obstructive pulmonary disease (COPD) are regulated by environmental and genetic factors. In hypoxia, Erythropoietin (EPO) satisfies the body’s need for oxygen by promoting the production of red blood cells. Hypoxia was proven to be a common physiological condition in COPD progression and associated with many complications. Some studies have found that EPO is involved in the development of COPD. But the mechanism has not been fully proven. Methods: We conducted a case-control study enrolled 1095 COPD patients and 1144 healthy controls in Guangdong Province to evaluate the association between EPO polymorphisms (rs1617640 A>C, rs507392 A>G, rs564449 G>T) and COPD susceptibility. 872 participants from southern Gansu Province were recruited to verify the effect of EPO polymorphisms on lung function. Results: EPO rs1617640 C allele reduced COPD susceptibility in southern Chinese significantly (AC vs. AA: adjusted Odds ratio (OR) = 0.805, 95% CI = 0.669–0.969; AC+CC vs. AA: adjusted OR = 0.822, 95% CI = 0.689–0.980). However, there was no association between rs507392 A>G and rs564449 G>T polymorphisms and COPD susceptibility (p > 0.05). We further observed that the rs1617640 C allele was associated with higher FEV1 and FVC in Guangdong and Gansu populations significantly (both p < 0.05). In brief, the level of FEV1 and FVC increased with the C allele number. We modeled the relative risk for men and women, in which the population-attributable risks chances were 0.449 (0.258–0.641) and 0.262 (0.128–0.396) respectively. In this model, smoking status, coal as fuels, education level, and rs1617640 A>C were finally retained for males, while smoking status, biomass as fuels, and1617640 A>C were retained for females. In the end, using the method developed by Gail and Bruzzi, we fitted a 10-year absolute risk model for southern Chinese with different individual relative risks, which was presented as a table. Conclusions: In conclusion, this study found that EPO rs1617640 A>C polymorphism is associated with COPD susceptibility in southern Chinese, and the C allele was associated with better lung function. In addition, it could also be considered a genetic marker associated with environmental factors to predict the absolute 10-year risk of COPD in southern Chinese.
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- 2023
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16. Automated and accurate assessment for postural abnormalities in patients with Parkinson’s disease based on Kinect and machine learning
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Zhuoyu Zhang, Ronghua Hong, Ao Lin, Xiaoyun Su, Yue Jin, Yichen Gao, Kangwen Peng, Yudi Li, Tianyu Zhang, Hongping Zhi, Qiang Guan, and LingJing Jin
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Parkinson’s disease ,Postural abnormalities ,Kinect ,Machine learning ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Abstract Background Automated and accurate assessment for postural abnormalities is necessary to monitor the clinical progress of Parkinson’s disease (PD). The combination of depth camera and machine learning makes this purpose possible. Methods Kinect was used to collect the postural images from 70 PD patients. The collected images were processed to extract three-dimensional body joints, which were then converted to two-dimensional body joints to obtain eight quantified coronal and sagittal features (F1-F8) of the trunk. The decision tree classifier was carried out over a data set established by the collected features and the corresponding doctors’ MDS-UPDRS-III 3.13 (the 13th item of the third part of Movement Disorder Society-Sponsored Revision of the Unified Parkinson’s Disease Rating Scale) scores. An objective function was implanted to further improve the human–machine consistency. Results The automated grading of postural abnormalities for PD patients was realized with only six selected features. The intraclass correlation coefficient (ICC) between the machine’s and doctors’ score was 0.940 (95%CI, 0.905–0.962), meaning the machine was highly consistent with the doctors’ judgement. Besides, the decision tree classifier performed outstandingly, reaching 90.0% of accuracy, 95.7% of specificity and 89.1% of sensitivity in rating postural severity. Conclusions We developed an intelligent evaluation system to provide accurate and automated assessment of trunk postural abnormalities in PD patients. This study demonstrates the practicability of our proposed method in the clinical scenario to help making the medical decision about PD.
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- 2021
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17. Associations between LMO1 gene polymorphisms and central nervous system tumor susceptibility
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Huiran Lin, Huitong Chen, Ao Lin, Xiaoping Liu, Xiaokai Huang, Jingying Zhou, Li Yuan, and Zhenjian Zhuo
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LMO1 ,SNPs ,CNS tumor ,Chinese ,Pediatrics ,RJ1-570 - Abstract
ABSTRACT Importance LIM domain only 1 (LMO1) gene polymorphisms were previously found to be implicated in the risk of several cancers. No available studies were performed regarding the predisposing effect of LMO1 gene single nucleotide polymorphisms (SNPs) on central nervous system (CNS) tumor risk. Objective We aimed to determine whether the LMO1 gene SNPs were associated with the risk of CNS tumor by applying a case‐control study with 191 cases and 248 controls in China. Methods The contributions of LMO1 gene SNPs to the risk of CNS tumor was evaluated by multinomial logistic regression. Results Based on the calculations of odds ratio (OR) and 95% confidence interval (CI), we failed to detect a significant relationship between each LMO1 gene SNP (rs110419 A>G, rs4758051 G>A, rs10840002 A>G, rs204938 A>G, and rs2168101 G>T) and CNS tumor risk, respectively. A negative association was also found in the combined effects on these five SNPs and CNS tumor risk. The stratification analysis further demonstrated the individuals with rs204938 AG/GG genotype confer to increased risk of CNS tumor compared with those with an AA genotype in males (OR: 1.74, 95% CI: 1.01–2.98, P = 0.046). Interpretation We concluded that LMO1 gene SNPs may not strong enough to influence the risk of CNS tumor in Chinese children. More studies are required to verify this association.
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- 2021
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18. Nomograms predict survival benefits of radical prostatectomy and chemotherapy for prostate cancer with bone metastases: A SEER‐based study
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Donglin Sun, Ao Lin, Zhun Sun, Shuqi Yang, Yuexin Sun, Anning Chen, Guojun Qian, Zhonghua Ji, and Li Wang
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prostate cancer ,bone metastasis ,prognosis ,nomogram ,radical prostatectomy ,chemotherapy ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
PurposeThis study aimed to identify independent prognosis-associated factors of bone-metastatic prostate cancer. The nomograms were further developed to obtain indicators for the prognostic evaluation.MethodsA total of 7315 bone-metastatic prostate cancer (PCa) patients from 2010 to 2016 were retrospectively collected from the Surveillance, Epidemiology, and End Results (SEER) database. Patients were randomly divided into the training cohort (n=5,120) and test cohort (n=2,195) in a ratio of 7:3. Univariate and multivariate Cox regression models were applied to evaluate potential risk factors. A 1:1 propensity score matching (PSM) was further performed to decrease the confounding effect and re-evaluate the influence of radical prostatectomy and chemotherapy on prognosis. Combining these potential prognosis factors, the nomograms of cancer-specific survival (CSS) and overall survival (OS) at different times were established. C-indexes, calibration curves, and decision curves were developed to evaluate the discrimination, calibration, and clinical benefit of the nomograms.ResultsEleven independent prognosis factors for CSS and twelve for OS were utilized to conduct the nomograms respectively. The C-indexes of nomograms for CSS and OS were 0.712 and 0.702, respectively. A favorable consistency between the predicted and actual survival probabilities was demonstrated by adopting calibration curves. Decision curves also exhibited a positive clinical benefit of the nomograms.ConclusionsNomograms were formulated successfully to predict 3-year and 5-year CSS and OS for bone-metastatic PCa patients. Radical prostatectomy and chemotherapy were strongly associated with the bone-metastatic PCa prognosis.
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- 2022
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19. Corrigendum: Rational design of a Gd(III)–Cu(II) nanobooster for chemodynamic therapy against cancer cells
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Xin-Ya Shi, Ting-Xiao Shen, Ao-Lin Zhang, Li-Tao Tan, Wen-Chang Shen, Hai-Jiang Zhong, Shun-Lin Zhang, Yu-Lan Gu, and Lei Shen
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tetrazole ,Gd(III)–Cu(II) ,crystal structure ,chemodynamic therapy ,nanobooster ,Chemistry ,QD1-999 - Published
- 2022
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20. Mitochondrial S‐adenosylmethionine deficiency induces mitochondrial unfolded protein response and extends lifespan in Caenorhabditis elegans
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Chen, Tse Yu, primary, Wang, Feng‐Yung, additional, Lee, Pin‐Jung, additional, Hsu, Ao‐Lin, additional, and Ching, Tsui‐Ting, additional
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- 2024
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21. Technology-based therapy-response evaluation of axial motor symptoms under daily drug regimen of patients with Parkinson’s disease
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Zhuang Wu, Ronghua Hong, Shuangfang Li, Kangwen Peng, Ao Lin, Yichen Gao, Yue Jin, Xiaoyun Su, Hongping Zhi, Qiang Guan, Lizhen Pan, and Lingjing Jin
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Parkinson’s disease ,depth camera ,objective measurement ,axial mobility ,motor improvement ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
BackgroundAxial disturbances are the most disabling symptoms of Parkinson’s disease (PD). Kinect-based objective measures could extract motion characteristics with high reliability and validity.PurposeThe present research aimed to quantify the therapy–response of axial motor symptoms to daily medication regimen and to explore the correlates of the improvement rate (IR) of axial motor symptoms based on a Kinect camera.Materials and methodsWe enrolled 44 patients with PD and 21 healthy controls. All 65 participants performed the Movement Disorder Society-Sponsored Revision of the Unified Parkinson’s Disease Rating Scale part III and the Kinect-based kinematic evaluation to assess arising from a chair, gait, posture, and postural stability before and after medication. Spearman’s correlation analysis and multiple linear regression model were performed to explore the relationships between motor feature IR and clinical data.ResultsAll the features arising from a chair (P = 0.001), stride length (P = 0.001), velocity (P < 0.001), the height of foot lift (P < 0.001), and turning time (P = 0.001) improved significantly after a daily drug regimen in patients with PD. In addition, the anterior trunk flexion (lumbar level) exhibited significant improvement (P = 0.004). The IR of the axial motor symptoms score was significantly correlated with the IRs of kinematic features for gait velocity, stride length, foot lift height, and sitting speed (rs = 0.345, P = 0.022; rs = 0.382, P = 0.010; rs = 0.314, P = 0.038; rs = 0.518, P < 0.001, respectively). A multivariable regression analysis showed that the improvement in axial motor symptoms was associated with the IR of gait velocity only (β = 0.593, 95% CI = 0.023–1.164, P = 0.042).ConclusionAxial symptoms were not completely drug-resistant, and some kinematic features can be improved after the daily medication regimen of patients with PD.
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- 2022
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22. Readmission Risk Prediction Model for Patients with Chronic Heart Failure: A Systematic Review and Meta-Analysis
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Jing Liu, Ping Liu, Mei-Rong Lei, Hong-Wei Zhang, Ao-Lin You, and Xiao-Rong Luan
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Chronic heart failure ,Readmission ,Prediction model ,Systematic review ,Public aspects of medicine ,RA1-1270 - Abstract
Background: The present systematic review and meta-analysis aimed to systematically evaluate a risk prediction model for the readmission of patients with CHF. Methods: The search was carried out in databases including PubMed, Embase, EBSCO, Web of Science, Cochrane Library and also domestic databases including Chinese Biomedical Literature Database, Chinese Academic Journal Full Text Database, Wanfang Database, and Vipu Chinese Journal Service Platform. All the original studies published by July 2021. Two researchers identified previous studies involving readmission risk prediction models that met our selection criteria. The quality of the included studies was evaluated based on the CHARMS checklist, and the prediction models were systematically evaluated. Results: Of the overall 4787 studies retrieved, nine studies—two prospective, seven retrospective—met our selection criteria. The area under the receiver operating characteristic curve exceeded 0.63 (0.63-0.80) for all the studies. The most common predictors in the model were B-type natriuretic peptide (BNP) or N-terminal pro-brain BNP (Odds Ratio 4.35; 95% confidence interval (CI) 2.53–7.49; P
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- 2022
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23. Caenorhabditis elegans learning in a structured maze is a multisensory behavior
- Author
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Gourgou, Eleni, Adiga, Kavya, Goettemoeller, Anne, Chen, Chieh, and Hsu, Ao-Lin
- Published
- 2021
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24. Rational Design of a Gd(III)–Cu(II) Nanobooster for Chemodynamic Therapy Against Cancer Cells
- Author
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Xin-Ya Shi, Ting-Xiao Shen, Ao-Lin Zhang, Li-Tao Tan, Wen-Chang Shen, Hai-Jiang Zhong, Shun-Lin Zhang, Yu-Lan Gu, and Lei Shen
- Subjects
tetrazole ,Gd(III)–Cu(II) ,crystal structure ,chemodynamic therapy ,nanobooster ,Chemistry ,QD1-999 - Abstract
Copper (II) containing coordination complexes have attracted much attention for chemodynamic therapy (CDT) against cancer cells. In this study, the bimetallic nanobooster [Gd2Cu(L)2(H2O)10]·6H2O was prepared by a solvothermal method based on tetrazole carboxylic acid ligand H4L [H4L = 3,3-di (1H-tetrazol-5-yl) pentanedioic acid]. It showed considerable cytotoxicity toward three kinds of human cancer cells (HeLa, HepG2, and HT29). The MTT assay showed that the IC50 (half-maximal inhibitory concentration) of the complex NPs on HeLa cells (4.9 μg/ml) is superior to that of HepG2 (11.1 μg/ml) and HT29 (5.5 μg/ml). This result showed that [Gd2Cu(L)2(H2O)10]·6H2O NPs can inhibit cell proliferation in vitro and may be potential candidates for chemodynamic therapy. In addition, the cytotoxicity was also confirmed by the trypan blue staining experiment. The results promise the great potential of Gd(III)–Cu(II) for CDT against cancer cells.
- Published
- 2022
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25. Genetic variants in m6A modification core genes are associated with glioma risk in Chinese children
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Jing He, Li Yuan, Huiran Lin, Ao Lin, Huitong Chen, Ailing Luo, Zhenjian Zhuo, and Xiaoping Liu
- Subjects
glioma ,m6A ,polymorphism ,susceptibility ,Chinese ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Glioma is a highly heritable disease with a strong genetic component. The N6-methyladenosine (m6A) modification core genes play important roles in the context of cancer. However, the effects of polymorphisms in the m6A modification core genes on the risk of pediatric glioma remain undefined. Here, we intended to demonstrate the relationship between 24 functional single-nucleotide polymorphisms (SNPs) in eight m6A modification core genes and glioma risk. Case-control design and multinomial logistic regression were used to develop models to estimate the risk of glioma while accounting for the subtypes of glioma. A total of 171 glioma cases and 228 controls from South China were genotyped using a TaqMan assay. The WTAP rs7766006, YTHDF2 rs3738067, and FTO rs9939609 variants conferred a statistically significant increased risk of glioma, respectively. YTHDC1 rs2293595, YTHDC1 rs3813832, and FTO rs8047395 were associated with a significant inverse association with risk of glioma, respectively. The significant associations were more predominant in stratification analyses of certain subgroups. Functional annotations revealed that WTAP rs7766006 and YTHDF2 rs3738067 could be potential functional variants by increasing expression of WTAP and YTHDF2 mRNA, respectively. Overall, these findings implicate variants in the m6A modification core genes as playing a role in pediatric glioma etiology.
- Published
- 2021
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26. Inter- and trans-generational impacts of real-world PM2.5 exposure on male-specific primary hypogonadism.
- Author
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Wei, Xiaoyu, Zhang, Zhonghao, Gu, Yayun, Zhang, Rong, Huang, Jie, Li, Feng, He, Yuanlin, Lu, Shuai, Wu, Yifei, Zeng, Wentao, Liu, Xiaorui, Liu, Chenzi, Liu, Jinyi, Ao, Lin, Shi, Fuquan, Chen, Qing, Lin, Yuan, Du, Jiangbo, Jin, Guangfu, and Xia, Yankai
- Subjects
MALE reproductive health ,HYPOGONADISM ,POLLUTANTS ,CELL physiology ,LEYDIG cells - Abstract
Exposure to PM
2.5 , a harmful type of air pollution, has been associated with compromised male reproductive health; however, it remains unclear whether such exposure can elicit transgenerational effects on male fertility. Here, we aim to examine the effect of paternal exposure to real-world PM2.5 on the reproductive health of male offspring. We have observed that paternal exposure to real-world PM2.5 can lead to transgenerational primary hypogonadism in a sex-selective manner, and we have also confirmed this phenotype by using an external model. Mechanically, we have identified small RNAs (sRNAs) that play a critical role in mediating these transgenerational effects. Specifically, miR6240 and piR016061, which are present in F0 PM sperm, regulate intergenerational transmission by targeting Lhcgr and Nsd1, respectively. We have also uncovered that piR033435 and piR006695 indirectly regulate F1 PM sperm methylation by binding to the 3′-untranslated region of Tet1 mRNA. The reduced expression of Tet1 resulted in hypermethylation of several testosterone synthesis genes, including Lhcgr and Gnas, impaired Leydig cell function and ultimately led to transgenerational primary hypogonadism. Our findings provide insights into the mechanisms underlying the transgenerational effects of paternal PM2.5 exposure on reproductive health, highlighting the crucial role played by sRNAs in mediating these effects. The findings underscore the significance of paternal pre-conception interventions in alleviating the adverse effects of environmental pollutants on reproductive health. [ABSTRACT FROM AUTHOR]- Published
- 2024
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27. Epigenetic Inactivation of SOX30 Is Associated with Male Infertility and Offers a Therapy Target for Non-obstructive Azoospermia
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Han, Fei, Jiang, Xiao, Li, Zhi-ming, Zhuang, Xuan, Zhang, Xi, Ouyang, Wei-ming, Liu, Wen-bin, Mao, Cheng-yi, Chen, Qing, Huang, Chuan-shu, Gao, Fei, Cui, Zhi-hong, Ao, Lin, Li, Yan-feng, Cao, Jia, and Liu, Jin-yi
- Published
- 2020
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28. The value of ICG-guided left colon vascular variation and anatomical rules for the radical resection of proctosigmoid colon cancer
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Wang, Jiahao, primary, Xie, Jiaxin, additional, Lu, Xinquan, additional, Lin, Jiaxin, additional, Liao, Weilin, additional, Yi, Xiaojiang, additional, Feng, Xiaochuang, additional, Zhu, Bosen, additional, Li, Wenjuan, additional, Tang, Xin, additional, Ao, Lin, additional, Chen, Zhifeng, additional, Li, Hongming, additional, and Diao, Dechang, additional
- Published
- 2023
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- View/download PDF
29. Reconstructing Depth Images for Time-of-Flight Cameras Based on Second-Order Correlation Functions
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Wang, Tian-Long, primary, Ao, Lin, additional, Zheng, Jie, additional, and Sun, Zhi-Bin, additional
- Published
- 2023
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30. Folate metabolism modifies chromosomal damage induced by 1,3-butadiene: results from a match-up study in China and in vitro experiments
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Xiang, Menglong, Wang, Zhi, Zou, Peng, Ling, Xi, Zhang, Guowei, Zhou, Ziyuan, Cao, Jia, and Ao, Lin
- Published
- 2021
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31. A maze platform for the assessment of Caenorhabditis elegans behavior and learning
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Eleni Gourgou and Ao-Lin Hsu
- Subjects
Model Organisms ,Neuroscience ,Behavior ,Science (General) ,Q1-390 - Abstract
Summary: Mazes are broadly used to investigate animal decision-making and spatial learning. However, they have been sparsely employed to explore C. elegans behavior and training-improved performance. This protocol describes a highly reproducible, low-cost maze platform, made of the standard, agar-based, nematode culturing material. It can be used to reliably assess C. elegans' maze behavior, and we have recently applied this protocol to establish multisensory learning. Limitations include challenges in locomotion tracking and in distinguishing learning formation versus retrieval.For complete details on the use and execution of this protocol, please refer to Gourgou et al. (2021).
- Published
- 2021
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32. Association Between Gene Polymorphisms and Glioma Susceptibility in Chinese Children
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Huiqin Guo BA, Yuxiang Liao MD, Ao Lin MS, Huiran Lin MS, Xiaokai Huang MS, Jichen Ruan MD, Li Yuan PhD, and Zhenjian Zhuo PhD
- Subjects
Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Gliomas are the most prevalent brain tumors among children and adolescents. The occurrence and development of various malignant tumors is closely related with LIN28A gene, but its relationship with glioma susceptibility has not been widely discovered. In this case-control study, we conducted four single nucleotide polymorphisms (SNPs) (rs3811464 G>A, rs3811463 T>C, rs34787247 G>A, and rs11247957 G>A) of LIN28A gene to investigate whether they increase the risk of glioma. Odds ratios (ORs) and 95% confidence intervals (CIs) were used to evaluate their relationship. There was no significant correlation between four SNPs and glioma risk in single polymorphism and conjoint analysis. However, in stratification analysis, we found that rs3811463 TC/CC may add to the risk of glioma with clinical stage III (adjusted OR = 3.16, 95% CI = 1.15-8.70, P = .026) or stage III+IV patients (adjusted OR = 2.05, 95% CI = 1.02-4.13, P = .044). Our research suggested that four SNPs of LIN28A gene have a weak relationship with the risk of glioma in Chinese children. LIN28A rs3811463 TC/CC may increase the possibility of glioma in clinical stage III or stage III+IV patients which need larger samples and further confirmation.
- Published
- 2021
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33. Caenorhabditis elegans learning in a structured maze is a multisensory behavior
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Eleni Gourgou, Kavya Adiga, Anne Goettemoeller, Chieh Chen, and Ao-Lin Hsu
- Subjects
Behavioral Neuroscience ,Biological Sciences ,Neuroscience ,Science - Abstract
Summary: We show that C. elegans nematodes learn to associate food with a combination of proprioceptive cues and information on the structure of their surroundings (maze), perceived through mechanosensation. By using the custom-made Worm-Maze platform, we demonstrate that C. elegans young adults can locate food in T-shaped mazes and, following that experience, learn to reach a specific maze arm. C. elegans learning inside the maze is possible after a single training session, it resembles working memory, and it prevails over conflicting environmental cues. We provide evidence that the observed learning is a food-triggered multisensory behavior, which requires mechanosensory and proprioceptive input, and utilizes cues about the structural features of nematodes' environment and their body actions. The CREB-like transcription factor and dopamine signaling are also involved in maze performance. Lastly, we show that the observed aging-driven decline of C. elegans learning ability in the maze can be reversed by starvation.
- Published
- 2021
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34. Distinct sets of lysosomal genes define synucleinopathy and tauopathy
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Oh, Kyu Won, primary, Kim, Dong-Kyu, additional, Hsu, Ao-Lin, additional, and Lee, Seung-Jae, additional
- Published
- 2023
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35. HSB-1 Inhibition and HSF-1 Overexpression Trigger Overlapping Transcriptional Changes To Promote Longevity in Caenorhabditis elegans
- Author
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Surojit Sural, Tzu-Chiao Lu, Seung Ah Jung, and Ao-Lin Hsu
- Subjects
C. elegans ,heat shock factor ,HSB-1 ,life span ,RNA-Seq ,Genetics ,QH426-470 - Abstract
Heat shock factor 1 (HSF-1) is a component of the heat shock response pathway that is induced by cytoplasmic proteotoxic stress. In addition to its role in stress response, HSF-1 also acts as a key regulator of the rate of organismal aging. Overexpression of HSF-1 promotes longevity in C. elegans via mechanisms that remain less understood. Moreover, genetic ablation of a negative regulator of HSF-1, termed as heat shock factor binding protein 1 (HSB-1), results in hsf-1-dependent life span extension in animals. Here we show that in the absence of HSB-1, HSF-1 acquires increased DNA binding activity to its genomic target sequence. Using RNA-Seq to compare the gene expression profiles of the hsb-1 mutant and hsf-1 overexpression strains, we found that while more than 1,500 transcripts show ≥1.5-fold upregulation due to HSF-1 overexpression, HSB-1 inhibition alters the expression of less than 500 genes in C. elegans. Roughly half of the differentially regulated transcripts in the hsb-1 mutant have altered expression also in hsf-1 overexpressing animals, with a strongly correlated fold-expression pattern between the two strains. In addition, genes that are upregulated via both HSB-1 inhibition and HSF-1 overexpression include numerous DAF-16 targets that have known functions in longevity regulation. This study identifies how HSB-1 acts as a specific regulator of the transactivation potential of HSF-1 in non-stressed conditions, thus providing a detailed understanding of the role of HSB-1/HSF-1 signaling pathway in transcriptional regulation and longevity in C. elegans.
- Published
- 2019
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36. Meta-analysis of the association between sclerostin level and adverse clinical outcomes in patients undergoing maintenance haemodialysis
- Author
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Sha-Sha Li, Zhi-Qin Zhang, Da-Wei He, Ao-Lin He, and Qi-Feng Liu
- Subjects
Therapeutics. Pharmacology ,RM1-950 - Abstract
Background: Studies regarding the relationship of sclerostin (Scl) with clinical outcomes in patients undergoing maintenance haemodialysis have yielded controversial findings. This meta-analysis was performed to investigate the predictive role of Scl in this patient population. Methods: Several electronic medical databases (e.g. PubMed, Embase, Web of Science and Cochrane Library) were searched for eligible studies through December 20, 2019. Summary hazard ratios (HRs) with 95% confidence intervals (CIs) were calculated based on Scr level (high or low) using a random or fixed effects model. Results: From among 641 initially screened publications, 16 eligible studies were included in this meta-analysis. A high Scl level was not associated with cardiovascular events [HR = 0.8 (95% CI, 0.42–1.35)] or all-cause mortality [HR = 0.93 (95% CI, 0.56–1.54)]. There was high heterogeneity, but no evidence of publication bias. Interestingly, a high Scl level was associated with reduced cardiovascular events [HR = 0.44 (95% CI, 0.29–0.69)] in the subgroup by shorter follow-up period or all-cause mortality [pooled HR = 0.58 (95% CI, 0.36–0.91)] by shorter dialysis vintage. Conclusion: This meta-analysis indicated that a high Scl level did not predict total clinical outcomes in patients undergoing maintenance haemodialysis despite survival benefits in the subgroups. The predictive role of Scl in these patients should be further evaluated in large prospective studies.
- Published
- 2021
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37. Rice straw pretreatment using deep eutectic solvents with different constituents molar ratios: Biomass fractionation, polysaccharides enzymatic digestion and solvent reuse
- Author
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Li, Ao-Lin, Hou, Xue-Dan, Lin, Kai-Peng, Zhang, Xuan, and Fu, Ming-Hui
- Published
- 2018
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38. TC2N, a novel oncogene, accelerates tumor progression by suppressing p53 signaling pathway in lung cancer
- Author
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Hao, Xiang-lin, Han, Fei, Zhang, Ning, Chen, Hong-qiang, Jiang, Xiao, Yin, Li, Liu, Wen-bin, Wang, Dan-dan, Chen, Jian-ping, Cui, Zhi-hong, Ao, Lin, Cao, Jia, and Liu, Jin-yi
- Published
- 2019
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39. Activation of <scp>eIF4E</scp> ‐binding‐protein‐1 rescues <scp>mTORC1</scp> ‐induced sarcopenia by expanding lysosomal degradation capacity
- Author
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Elisa M. Crombie, Seonyoung Kim, Stuart Adamson, Han Dong, Tzu‐Chiao Lu, Yiju Wu, Yajun Wu, Yotam Levy, Nolan Stimple, Wing Moon R. Lam, Hwee Weng D. Hey, Dominic J. Withers, Ao‐Lin Hsu, Boon Huat Bay, Julien Ochala, and Shih‐Yin Tsai
- Subjects
sarcopenia ,mRNA translation ,Physiology (medical) ,mitochondrial dysfunction ,protein degradation ,Orthopedics and Sports Medicine ,mTORC1 - Abstract
Background: Chronic mTORC1 activation in skeletal muscle is linked with age-associated loss of muscle mass and strength, known as sarcopenia. Genetic activation of mTORC1 by conditionally ablating mTORC1 upstream inhibitor TSC1 in skeletal muscle accelerates sarcopenia development in adult mice. Conversely, genetic suppression of mTORC1 downstream effectors of protein synthesis delays sarcopenia in natural aging mice. mTORC1 promotes protein synthesis by activating ribosomal protein S6 kinases (S6Ks) and inhibiting eIF4E-binding proteins (4EBPs). Whole-body knockout of S6K1 or muscle-specific over-expression of a 4EBP1 mutant transgene (4EBP1mt), which is resistant to mTORC1-mediated inhibition, ameliorates muscle loss with age and preserves muscle function by enhancing mitochondria activities, despite both transgenic mice showing retarded muscle growth at a young age. Why repression of mTORC1-mediated protein synthesis can mitigate progressive muscle atrophy and dysfunction with age remains unclear. Methods: Mice with myofiber-specific knockout of TSC1 (TSC1mKO), in which mTORC1 is hyperactivated in fully differentiated myofibers, were used as a mouse model of sarcopenia. To elucidate the role of mTORC1-mediated protein synthesis in regulating muscle mass and physiology, we bred the 4EBP1mt transgene or S6k1 floxed mice into the TSC1mKO mouse background to generate 4EBP1mt-TSC1mKO or S6K1-TSC1mKO mice, respectively. Functional and molecular analyses were performed to assess their role in sarcopenia development. Results: Here, we show that 4EBP1mt-TSC1mKO, but not S6K1-TSC1mKO, preserved muscle mass (36.7% increase compared with TSC1mKO, P
- Published
- 2022
40. Electroacupuncture-Related Metabolic Brain Connectivity in Neuropathic Pain due to Brachial Plexus Avulsion Injury in Rats
- Author
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Ao-Lin Hou, Mou-Xiong Zheng, Xu-Yun Hua, Bei-Bei Huo, Jun Shen, and Jian-Guang Xu
- Subjects
neuropathic pain ,brachial plexus avulsion injury ,electroacupuncture ,thermal withdrawal latency ,metabolic connectivity ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Objective: The present study aimed to investigate the analgesic effect of electroacupuncture (EA) in neuropathic pain due to brachial plexus avulsion injury (BPAI) and related changes in the metabolic brain connectivity.Methods: Neuropathic pain model due to BPAI was established in adult female Sprague–Dawley rats. EA stimulations (2/15 Hz, 30 min/day, 5-day intervention followed by 2-day rest in each session) were applied to the fifth–seventh cervical “Jiaji” acupoints on the noninjured side from 1st to 12th weeks following BPAI (EA group, n = 8). Three control groups included sham EA (nonelectrical acupuncture applied to 3 mm lateral to the real “Jiaji” acupoints), BPAI-only, and normal rats (no particular intervention; eight rats in each group). Thermal withdrawal latency (TWL) of the noninjured forepaw was regularly tested to evaluate the threshold of thermalgesia. Small animal [fluorine-18]-fluoro-2-deoxy-D-glucose (18F-FDG) PET/CT scans of brain were conducted at the end of 4th, 12th, and 16th weeks to explore metabolic alterations of brain.Results: In the EA group, the TWL of the noninjured forepaw significantly decreased following BPAI and then increased following EA stimulation, compared with sham EA (P < 0.001). The metabolic brain connectivity among somatosensory cortex (SC), motor cortex (MC), caudate putamen (Cpu), and dorsolateral thalamus (DLT) in bilateral hemispheres decreased throughout the 16 weeks’ observation in the BPAI-only group, compared with the normal rats (P < 0.05). In the EA group, the strength of connectivity among the above regions were found to be increased at the end of 4th week following BPAI modeling, decreased at 12th week, and then increased again at 16th week (P < 0.05). The changes in metabolic connectivity were uncharacteristic and dispersed in the sham EA group.Conclusion: The study revealed long-term and extensive changes of metabolic brain connectivity in EA-treated BPAI-induced neuropathic pain rats. Bilateral sensorimotor and pain-related brain regions were mainly involved in this process. It indicated that modulation of brain metabolic connectivity might be an important mechanism of analgesic effect in EA stimulation for the treatment of neuropathic pain.
- Published
- 2020
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- View/download PDF
41. S-adenosyl methionine synthetase SAMS-5 mediates dietary restriction-induced longevity in Caenorhabditis elegans.
- Author
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Chia-Chang Chen, Chiao Yin Lim, Pin-Jung Lee, Ao-Lin Hsu, and Tsui-Ting Ching
- Subjects
Medicine ,Science - Abstract
S-adenosyl methionine synthetase (SAMS) catalyzes the biosynthesis of S-adenosyl methionine (SAM), which serves as a universal methyl group donor for numerous biochemical reactions. Previous studies have clearly demonstrated that SAMS-1, a C. elegans homolog of mammalian SAMS, is critical for dietary restriction (DR)-induced longevity in Caenorhabditis elegans. In addition to SAMS-1, three other SAMS paralogs have been identified in C. elegans. However, their roles in longevity regulation have never been explored. Here, we show that depletion of sams-5, but not sams-3 or sams-4, can extend lifespan in worms. However, the phenotypes and expression pattern of sams-5 are distinct from sams-1, suggesting that these two SAMSs might regulate DR-induced longevity via different mechanisms. Through the genetic epistasis analysis, we have identified that sams-5 is required for DR-induced longevity in a pha-4/FOXA dependent manner.
- Published
- 2020
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42. Sperm telomere length is associated with sperm nuclear DNA integrity and mitochondrial DNA abnormalities among healthy male college students in Chongqing, China
- Author
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Ling, Xi, primary, Cui, Haonan, additional, Chen, Qing, additional, Yang, Wang, additional, Zou, Peng, additional, Yang, Huan, additional, Zhou, Niya, additional, Deng, Jiuyang, additional, Liu, Jinyi, additional, Cao, Jia, additional, and Ao, Lin, additional
- Published
- 2023
- Full Text
- View/download PDF
43. Regulation of Aging and Age-Related Disease by DAF-16 and Heat-Shock Factor
- Author
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Hsu, Ao-Lin, Murphy, Coleen T., and Kenyon, Cynthia
- Published
- 2003
44. Activation of eIF4E-binding-protein-1 rescues mTORC1-induced sarcopenia by expanding lysosomal degradation capacity
- Author
-
Crombie, Elisa M., Kim, Seonyoung, Adamson, Stuart, Dong, Han, Lu, Tzu Chiao, Wu, Yiju, Wu, Yajun, Levy, Yotam, Stimple, Nolan, Lam, Wing Moon R., Hey, Hwee Weng D., Withers, Dominic J., Hsu, Ao Lin, Bay, Boon Huat, Ochala, Julien, Tsai, Shih Yin, Crombie, Elisa M., Kim, Seonyoung, Adamson, Stuart, Dong, Han, Lu, Tzu Chiao, Wu, Yiju, Wu, Yajun, Levy, Yotam, Stimple, Nolan, Lam, Wing Moon R., Hey, Hwee Weng D., Withers, Dominic J., Hsu, Ao Lin, Bay, Boon Huat, Ochala, Julien, and Tsai, Shih Yin
- Abstract
Background: Chronic mTORC1 activation in skeletal muscle is linked with age-associated loss of muscle mass and strength, known as sarcopenia. Genetic activation of mTORC1 by conditionally ablating mTORC1 upstream inhibitor TSC1 in skeletal muscle accelerates sarcopenia development in adult mice. Conversely, genetic suppression of mTORC1 downstream effectors of protein synthesis delays sarcopenia in natural aging mice. mTORC1 promotes protein synthesis by activating ribosomal protein S6 kinases (S6Ks) and inhibiting eIF4E-binding proteins (4EBPs). Whole-body knockout of S6K1 or muscle-specific over-expression of a 4EBP1 mutant transgene (4EBP1mt), which is resistant to mTORC1-mediated inhibition, ameliorates muscle loss with age and preserves muscle function by enhancing mitochondria activities, despite both transgenic mice showing retarded muscle growth at a young age. Why repression of mTORC1-mediated protein synthesis can mitigate progressive muscle atrophy and dysfunction with age remains unclear. Methods: Mice with myofiber-specific knockout of TSC1 (TSC1mKO), in which mTORC1 is hyperactivated in fully differentiated myofibers, were used as a mouse model of sarcopenia. To elucidate the role of mTORC1-mediated protein synthesis in regulating muscle mass and physiology, we bred the 4EBP1mt transgene or S6k1 floxed mice into the TSC1mKO mouse background to generate 4EBP1mt-TSC1mKO or S6K1-TSC1mKO mice, respectively. Functional and molecular analyses were performed to assess their role in sarcopenia development. Results: Here, we show that 4EBP1mt-TSC1mKO, but not S6K1-TSC1mKO, preserved muscle mass (36.7% increase compared with TSC1mKO, P < 0.001) and strength (36.8% increase compared with TSC1mKO, P < 0.01) at the level of control mice. Mechanistically, 4EBP1 activation suppressed aberrant protein synthesis (two-fold reduction compared with TSC1mKO, P < 0.05) and restored autophagy flux without relieving mTORC1-mediated inhibition of ULK1, an upstream
- Published
- 2023
45. Rates of Behavior and Aging Specified by Mitochondrial Function during Development
- Author
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Dillin, Andrew, Hsu, Ao-Lin, Arantes-Oliveira, Nuno, Lehrer-Graiwer, Joshua, Hsin, Honor, Fraser, Andrew G., Kamath, Ravi S., Ahringer, Julie, and Kenyon, Cynthia
- Published
- 2002
46. A noise-resisted scheme of dynamical decoupling pulses for quantum memories
- Author
-
Gong, Bo, Tu, Tao, Zhu, Xing-Yu, Guo, Ao-lin, Zhou, Zong-quan, Guo, Guang-Can, and Li, Chuan-Feng
- Published
- 2020
- Full Text
- View/download PDF
47. Spin-photon module for scalable network architecture in quantum dots
- Author
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Zhu, Xing-Yu, Tu, Tao, Guo, Ao-Lin, Zhou, Zong-Quan, Guo, Guang-Can, and Li, Chuan-Feng
- Published
- 2020
- Full Text
- View/download PDF
48. Research on Trimming Frequency-Increasing Technology for Quartz Crystal Resonator Using Laser Etching
- Author
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Jun-Lin Zhang, Shuang Liao, Cheng Chen, Xiu-Tao Yang, Sheng-Ao Lin, Feng Tan, Bing Li, Wen-Wu Wang, Zheng-Xiang Zhong, and Guang-Gen Zeng
- Subjects
quartz crystal resonator (QCR) ,laser etching ,trimming frequency-increasing technology ,Mechanical engineering and machinery ,TJ1-1570 - Abstract
A quartz crystal resonator (QCR) is an indispensable electronic component in the field of the modern electronics industry. By designing and depositing electrodes of different shapes and thicknesses on a quartz wafer with a certain fundamental frequency, the desired target frequency can be obtained. Affected by factors such as the deposition equipment, mask, wafer size and placement position, it is difficult to accurately obtain the target frequency at a given time, especially for mass-produced QCRs. In this work, a laser with a wavelength of 532 nm was used to thin the electrodes of a QCR with a fundamental frequency of 10 MHz. The electrode surface was etched through a preset processing pattern to form a processing method of local thinning of the electrode surface. At the same time, the effect of laser etching on silicon dioxide and resonator performance was analyzed. Satisfactory trimming frequency-increasing results were achieved, such as a frequency modulation accuracy of 1 ppm, frequency distribution with good consistency and equivalent parameters with small changes, by the laser partial etching of the resonator electrode. However, when the surface electrode was etched into using through-holes, the attenuation amplitude of the equivalent parameter became larger, especially in terms of the quality factor (Q), which decreased from 63 K to 1 K, and some resonators which had a serious frequency drift of >40%. In this case, a certain number of QCRs were no longer excited to vibrate, which was due to the disappearance of the piezoelectric effect caused by the local thermal phase change in the quartz wafer.
- Published
- 2021
- Full Text
- View/download PDF
49. Simplified lung ultrasound method to predict the respiratory support needs of neonates whose mothers had pregnancy complications: A diagnostic accuracy study
- Author
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Xin-Ao Lin, Xue-Feng Wang, Xiao-Bo Fan, Guan-Nan Xi, and Ji-Mei Wang
- Subjects
Reproductive Medicine ,Obstetrics and Gynecology - Published
- 2023
50. New genes tied to endocrine, metabolic, and dietary regulation of lifespan from a Caenorhabditis elegans genomic RNAi screen.
- Author
-
Hansen, Malene, Hsu, Ao-Lin, Dillin, Andrew, and Kenyon, Cynthia
- Subjects
Genetics ,Developmental Biology - Abstract
Most of our knowledge about the regulation of aging comes from mutants originally isolated for other phenotypes. To ask whether our current view of aging has been affected by selection bias, and to deepen our understanding of known longevity pathways, we screened a genomic Caenorhabditis elegans RNAi library for clones that extend lifespan. We identified 23 new longevity genes affecting signal transduction, the stress response, gene expression, and metabolism and assigned these genes to specific longevity pathways. Our most important findings are (i) that dietary restriction extends C. elegans' lifespan by down-regulating expression of key genes, including a gene required for methylation of many macromolecules, (ii) that integrin signaling is likely to play a general, evolutionarily conserved role in lifespan regulation, and (iii) that specific lipophilic hormones may influence lifespan in a DAF-16/FOXO-dependent fashion. Surprisingly, of the new genes that have conserved sequence domains, only one could not be associated with a known longevity pathway. Thus, our current view of the genetics of aging has probably not been distorted substantially by selection bias.
- Published
- 2005
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