8 results on '"Amiel, Aurélien"'
Search Results
2. Arterio-venous metabolomics exploration reveals major changes across liver and intestine in the obese Yucatan minipig
- Author
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Poupin, Nathalie, Tremblay-Franco, Marie, Amiel, Aurélien, Canlet, Cécile, Rémond, Didier, Debrauwer, Laurent, Dardevet, Dominique, Thiele, Ines, Aurich, Maike K., Jourdan, Fabien, Savary-Auzeloux, Isabelle, and Polakof, Sergio
- Published
- 2019
- Full Text
- View/download PDF
3. Postprandial NMR-Based Metabolic Exchanges Reflect Impaired Phenotypic Flexibility across Splanchnic Organs in the Obese Yucatan Mini-Pig
- Author
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Tremblay-Franco, Marie, primary, Poupin, Nathalie, additional, Amiel, Aurélien, additional, Canlet, Cécile, additional, Rémond, Didier, additional, Debrauwer, Laurent, additional, Dardevet, Dominique, additional, Jourdan, Fabien, additional, Savary-Auzeloux, Isabelle, additional, and Polakof, Sergio, additional
- Published
- 2020
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- View/download PDF
4. Proton NMR Enables the Absolute Quantification of Aqueous Metabolites and Lipid Classes in Unique Mouse Liver Samples
- Author
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Amiel, Aurélien, primary, Tremblay-Franco, Marie, additional, Gautier, Roselyne, additional, Ducheix, Simon, additional, Montagner, Alexandra, additional, Polizzi, Arnaud, additional, Debrauwer, Laurent, additional, Guillou, Hervé, additional, Bertrand-Michel, Justine, additional, and Canlet, Cécile, additional
- Published
- 2019
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5. Comparison of the Phytochemical Composition of Serenoa repens Extracts by a Multiplexed Metabolomic Approach
- Author
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Marti, Guillaume, Joulia, Philippe, Amiel, Aurélien, Fabre, Bernard, David, Bruno, Fabre, Nicolas, Fiorini-Puybaret, Christel, Pharmacochimie et Biologie pour le Développement (PHARMA-DEV), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie de Toulouse (ICT-FR 2599), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut de Chimie du CNRS (INC)-Institut de Recherche pour le Développement (IRD), Centre de Recherche Pierre Fabre (Centre de R&D Pierre Fabre), PIERRE FABRE, Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), and Université de Toulouse (UT)
- Subjects
MESH: Mass Spectrometry ,MESH: Glycerophospholipids / chemistry ,phytochemical equivalence ,Saw palmetto ,MESH: Polyphenols / chemistry ,natural products ,[SDV]Life Sciences [q-bio] ,MESH: Plant Extracts / chemistry ,metabolomics ,lcsh:QD241-441 ,MESH: Serenoa / chemistry ,lcsh:Organic chemistry ,MESH: Fatty Acids / chemistry ,Serenoa repens extract ,MESH: Biological Products / chemistry ,MESH: Phytochemicals / chemistry ,MESH: Metabolomics / methods - Abstract
Phytochemical extracts are highly complex chemical mixtures. In the context of an increasing demand for phytopharmaceuticals, assessment of the phytochemical equivalence of extraction procedures is of utmost importance. Compared to routine analytical methods, comprehensive metabolite profiling has pushed forward the concept of phytochemical equivalence. In this study, an untargeted metabolomic approach was used to cross-compare four marketed extracts from Serenoa repens obtained with three different extraction processes: ethanolic, hexanic and sCO2 (supercritical carbon dioxide). Our approach involved a biphasic extraction of native compounds followed by liquid chromatography coupled to a high-resolution mass spectrometry based metabolomic workflow. Our results showed significant differences in the contents of major and minor compounds according to the extraction solvent used. The analyses showed that ethanolic extracts were supplemented in phosphoglycerides and polyphenols, hexanic extracts had higher amounts of free fatty acids and minor compounds, and sCO2 samples contained more glycerides. The discriminant model in this study could predict the extraction solvent used in commercial samples and highlighted the specific biomarkers of each process. This metabolomic survey allowed the authors to assess the phytochemical content of extracts and finished products of S. repens and unequivocally established that sCO2, hexanic and ethanolic extracts are not chemically equivalent and are therefore unlikely to be pharmacologically equivalent.
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- 2019
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6. Root-associated Streptomycesproduce galbonolides to modulate plant immunity and promote rhizosphere colonization
- Author
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Nicolle, Clément, Gayrard, Damien, Noël, Alba, Hortala, Marion, Amiel, Aurélien, Grat, Sabine, Le Ru, Aurélie, Marti, Guillaume, Pernodet, Jean-Luc, Lautru, Sylvie, Dumas, Bernard, and Rey, Thomas
- Abstract
The rhizosphere, which serves as the primary interface between plant roots and the soil, constitutes an ecological niche for a huge diversity of microbial communities. Currently, there is little knowledge on the nature and the function of the different metabolites released by rhizospheric microbes to facilitate colonization of this highly competitive environment. Here, we demonstrate how the production of galbonolides, a group of polyene macrolides that inhibit plant and fungal inositol phosphorylceramide synthase (IPCS), empowers the rhizospheric Streptomycesstrain AgN23, to thrive in the rhizosphere by triggering the plant’s defence mechanisms. Metabolomic analysis of AgN23-inoculated Arabidopsisroots revealed a strong induction in the production of an indole alkaloid, camalexin, which is a major phytoalexin in Arabidopsis. By using a plant mutant compromised in camalexin synthesis, we show that camalexin production is necessary for the successful colonization of the rhizosphere by AgN23. Conversely, hindering galbonolides biosynthesis in AgN23 knock-out mutant resulted in loss of inhibition of IPCS, a deficiency in plant defence activation, notably the production of camalexin, and a strongly reduced development of the mutant bacteria in the rhizosphere. Together, our results identified galbonolides as important metabolites mediating rhizosphere colonization by Streptomyces.Graphical AbstractModel summarizing the mode of action of galbonolides in stimulating plant defence to support AgN23 colonization of the rhizosphere.Galbonolides secretion by Streptomycessp. AgN23 trigger inositol phosphorylceramide synthase (IPCS) inhibition in Arabidopsisroot cells (orange arrow). The resulting raise in Ceramide precursors of the IPCS may result in the different defence responses associated to AgN23: Hypersensitive Responses (HR), Salicylic Acid (SA) signalling, nuclear Ca2+influx, defence gene expression and camalexin biosynthesis. This production of camalexin (blue arrow) exert a positive effect on AgN23 growth in the rhizosphere, presumably by restricting the growth of bacterial and fungal competitors sensitive to this phytoalexin. In addition, galbonolides secretion in the rhizosphere may also directly interfere with fungal competitors of AgN23. The illustration was created with BioRender.com.
- Published
- 2024
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7. Proton NMR Enables the Absolute Quantification of Aqueous Metabolites and Lipid Classes in Unique Mouse Liver Samples.
- Author
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Amiel, Aurélien, Tremblay-Franco, Marie, Gautier, Roselyne, Ducheix, Simon, Montagner, Alexandra, Polizzi, Arnaud, Debrauwer, Laurent, Guillou, Hervé, Bertrand-Michel, Justine, and Canlet, Cécile
- Subjects
SATURATED fatty acids ,LACTATES ,PROTON magnetic resonance ,MONOUNSATURATED fatty acids ,NUCLEAR magnetic resonance ,ORGANIC solvents ,HYDROXYCHOLESTEROLS ,LIPIDS - Abstract
Hepatic metabolites provide valuable information on the physiological state of an organism, and thus, they are monitored in many clinical situations. Typically, monitoring requires several analyses for each class of targeted metabolite, which is time consuming. The present study aimed to evaluate a proton nuclear magnetic resonance (
1 H-NMR) method for obtaining quantitative measurements of aqueous and lipidic metabolites. We optimized the extraction protocol, the standard samples, and the organic solvents for the absolute quantification of lipid species. To validate the method, we analyzed metabolic profiles in livers of mice fed three different diets. We compared our results with values obtained with conventional methods and found strong correlations. The1 H-NMR protocol enabled the absolute quantification of 29 aqueous metabolites and eight lipid classes. Results showed that mice fed a diet enriched in saturated fatty acids had higher levels of triglycerides, cholesterol ester, monounsaturated fatty acids, lactate, 3-hydroxy-butyrate, and alanine and lower levels of glucose, compared to mice fed a control diet. In conclusion, proton NMR provided a rapid overview of the main lipid classes (triglycerides, cholesterol, phospholipids, fatty acids) and the most abundant aqueous metabolites in liver. [ABSTRACT FROM AUTHOR]- Published
- 2020
- Full Text
- View/download PDF
8. Root-associated Streptomyces produce galbonolides to modulate plant immunity and promote rhizosphere colonization.
- Author
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Nicolle C, Gayrard D, Noël A, Hortala M, Amiel A, Grat S, Le Ru A, Marti G, Pernodet JL, Lautru S, Dumas B, and Rey T
- Subjects
- Macrolides metabolism, Thiazoles metabolism, Soil Microbiology, Phytoalexins, Streptomyces metabolism, Streptomyces genetics, Arabidopsis microbiology, Arabidopsis genetics, Rhizosphere, Plant Roots microbiology, Indoles metabolism, Plant Immunity
- Abstract
The rhizosphere, which serves as the primary interface between plant roots and the soil, constitutes an ecological niche for a huge diversity of microbial communities. Currently, there is little knowledge on the nature and the function of the different metabolites released by rhizospheric microbes to facilitate colonization of this highly competitive environment. Here, we demonstrate how the production of galbonolides, a group of polyene macrolides that inhibit plant and fungal inositol phosphorylceramide synthase (IPCS), empowers the rhizospheric Streptomyces strain AgN23, to thrive in the rhizosphere by triggering the plant's defence mechanisms. Metabolomic analysis of AgN23-inoculated Arabidopsis roots revealed a strong induction in the production of an indole alkaloid, camalexin, which is a major phytoalexin in Arabidopsis. By using a plant mutant compromised in camalexin synthesis, we show that camalexin production is necessary for the successful colonization of the rhizosphere by AgN23. Conversely, hindering galbonolides biosynthesis in AgN23 knock-out mutant resulted in loss of inhibition of IPCS, a deficiency in plant defence activation, notably the production of camalexin, and a strongly reduced development of the mutant bacteria in the rhizosphere. Together, our results identified galbonolides as important metabolites mediating rhizosphere colonization by Streptomyces., (© The Author(s) 2024. Published by Oxford University Press on behalf of the International Society for Microbial Ecology.)
- Published
- 2024
- Full Text
- View/download PDF
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