1. Antioxidant and renal protective effects of Nano-selenium on adenine-induced acute renal failure in canines.
- Author
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Zhang M, Gao J, Kulyar MF, Luo W, Zhang G, Yang X, Zhang T, Gao H, Peng Y, Zhang J, Altaf M, Algharib SA, Zhou D, and He J
- Subjects
- Animals, Dogs, Male, NF-E2-Related Factor 2 metabolism, NF-E2-Related Factor 2 genetics, Nanoparticles, Kelch-Like ECH-Associated Protein 1 metabolism, Kelch-Like ECH-Associated Protein 1 genetics, Oxidative Stress drug effects, Protective Agents pharmacology, Acute Kidney Injury chemically induced, Antioxidants, Selenium, Kidney drug effects, Kidney pathology
- Abstract
Acute renal failure is a common clinical disease in canines, affecting antioxidant levels and decreasing the body's resistance. This study aims to explore the therapeutic mechanism of Nano-selenium in acute renal failure. The histopathological and imaging changes of kidney tissue were observed with the gene and protein expression levels of Keap1, Nrf2, HO-1, and NQO1 in the kidney. According to our findings, adding nano-selenium can effectively reduce the concentration of CRE and BUN in blood and kidney tissues. It increased the activity of GSH-PX and SOD by an effective reduction of MDA. Through pathological and imaging observations, it was found that adding nano-selenium could improve the kidney tissue structure of acute renal failure. The results of the RT-qPCR experiment showed that after the addition of nano-selenium, the mRNA expression of the Keap1 gene decreased significantly. In contrast, the mRNA expression of the Nrf2, HO-1, and NQO1 genes increased significantly. The experimental results were further verified by western blot and immunohistochemical analysis. Hence, the nano-selenium intervention improved kidney function and increased antioxidant levels in canines suffering from acute renal failure with the involvement of the Keap1-Nrf2/ARE signaling pathway., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Inc.)
- Published
- 2024
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