579 results on '"A. Poelaert"'
Search Results
2. A deep learning approach predicting the activity of COVID-19 therapeutics and vaccines against emerging variants
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Robert P. Matson, Isin Y. Comba, Eli Silvert, Michiel J. M. Niesen, Karthik Murugadoss, Dhruti Patwardhan, Rohit Suratekar, Elizabeth-Grace Goel, Brittany J. Poelaert, Kanny K. Wan, Kyle R. Brimacombe, AJ Venkatakrishnan, and Venky Soundararajan
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Biology (General) ,QH301-705.5 - Abstract
Abstract Understanding which viral variants evade neutralization is crucial for improving antibody-based treatments, especially with rapidly evolving viruses like SARS-CoV-2. Yet, conventional assays are labor intensive and cannot capture the full spectrum of variants. We present a deep learning approach to predict changes in neutralizing antibody activity of COVID-19 therapeutics and vaccine-elicited sera/plasma against emerging viral variants. Our approach leverages data of 67,885 unique SARS-CoV-2 Spike sequences and 7,069 in vitro assays. The resulting model accurately predicted fold changes in neutralizing activity (R2 = 0.77) for a test set (N = 980) of data collected up to eight months after the training data. Next, the model was used to predict changes in activity of current therapeutic and vaccine-induced antibodies against emerging SARS-CoV-2 lineages. Consistent with other work, we found significantly reduced activity against newer XBB descendants, notably EG.5, FL.1.5.1, and XBB.1.16; primarily attributed to the F456L spike mutation.
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- 2024
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3. The Value of Rotavirus Vaccination in Europe: A Call for Action
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Cohen, Robert, Martinón-Torres, Federico, Posiuniene, Inga, Benninghoff, Bernd, Oh, Kyu-Bin, and Poelaert, Dirk
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- 2023
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4. Visualization of the Facial Nerve with Ultra-high–Frequency Ultrasound
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Jeroen Poelaert, MD, Renaat Coopman, MD, DDS, PhD, Matthias Ureel, MD, DDS, Nicolas Dhooghe, MD, Eva Genbrugge, MD, Tana Mwewa, MD, Phillip Blondeel, MD, PhD, and Hubert Vermeersch, MD, PhD
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Surgery ,RD1-811 - Abstract
Background:. Profound variations in facial nerve branching, combined with the severe impact of facial palsy on the patient’s quality of life, make surgery in this region challenging. Recent advancements in ultrasound (US) technology, including the improved visualization of small structures, have led to a sharp increase in its medical indications in various medical disciplines. We aimed to prove the feasibility of using ultra-high–frequency (UHF) US to visualize the facial nerve and to guide surgeons during surgery on and around the facial nerve. Methods:. A cadaveric study was performed on one hemi-face with a UHF US imaging system and state-of-the-art transducers. Firstly, a transcutaneous US was performed, and the facial nerve branches of interest (zygomatic, buccal, and marginal mandibular branches) were marked using US-guided color-injections of filler mixed with methylene blue. Skin and subcutaneous fat were then removed to simulate the intraoperative field. Secondly, an “intraoperative” US examination was performed, and the same branches were marked by US-guided color-injections of filler mixed with indocyanine green. Anterograde facial nerve dissection was performed, and the distance between the nerve branches and the injected filler was measured. Results:. All color-injections (mixed with both methylene blue and indocyanine green) were positioned right next to the nerve branches (
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- 2023
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5. Impact of fatigue on anaesthesia providers: a scoping review
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Scholliers, Annelies, Cornelis, Stef, Tosi, Maurizio, Opsomer, Tine, Shaproski, David, Vanlersberghe, Caroline, Vanhonacker, Domien, Poelaert, Jan, Goudman, Lisa, and Moens, Maarten
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- 2023
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6. Visualization of the Facial Nerve with Ultra-high–Frequency Ultrasound
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Poelaert, Jeroen, Coopman, Renaat, Ureel, Matthias, Dhooghe, Nicolas, Genbrugge, Eva, Mwewa, Tana, Blondeel, Phillip, and Vermeersch, Hubert
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- 2023
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7. Enterovirus D-68 Infection of Primary Rat Cortical Neurons: Entry, Replication, and Functional Consequences
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Katrien C. K. Poelaert, Regina G. D. M. van Kleef, Mengying Liu, Arno van Vliet, Heyrhyoung Lyoo, Lora-Sophie Gerber, Yoshiki Narimatsu, Christian Büll, Henrik Clausen, Erik de Vries, Remco H. S. Westerink, and Frank J. M. van Kuppeveld
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EV-D68 ,neurotropism ,receptor ,Microbiology ,QR1-502 - Abstract
ABSTRACT Enterovirus D68 (EV-D68) is an emerging pathogen associated with mild to severe respiratory disease. Since 2014, EV-D68 is also linked to acute flaccid myelitis (AFM), causing paralysis and muscle weakness in children. However, it remains unclear whether this is due to an increased pathogenicity of contemporary EV-D68 clades or increased awareness and detection of this virus. Here, we describe an infection model of primary rat cortical neurons to study the entry, replication, and functional consequences of different EV-D68 strains, including historical and contemporary strains. We demonstrate that sialic acids are important (co)receptors for infection of both neurons and respiratory epithelial cells. Using a collection of glycoengineered isogenic HEK293 cell lines, we show that sialic acids on either N-glycans or glycosphingolipids can be used for infection. Additionally, we show that both excitatory glutamatergic and inhibitory GABA-ergic neurons are susceptible and permissive to historical and contemporary EV-D68 strains. EV-D68 infection of neurons leads to the reorganization of the Golgi-endomembranes forming replication organelles, first in the soma and later in the processes. Finally, we demonstrate that the spontaneous neuronal activity of EV-D68-infected neuronal network cultured on microelectrode arrays (MEA) is decreased, independent of the virus strain. Collectively, our findings provide novel insights into neurotropism and -pathology of different EV-D68 strains, and argue that it is unlikely that increased neurotropism is a recently acquired phenotype of a specific genetic lineage. IMPORTANCE Acute flaccid myelitis (AFM) is a serious neurological illness characterized by muscle weakness and paralysis in children. Since 2014, outbreaks of AFM have emerged worldwide, and they appear to be caused by nonpolio enteroviruses, particularly enterovirus-D68 (EV-D68), an unusual enterovirus that is known to mainly cause respiratory disease. It is unknown whether these outbreaks reflect a change of EV-D68 pathogenicity or are due to increased detection and awareness of this virus in recent years. To gain more insight herein, it is crucial to define how historical and circulating EV-D68 strains infect and replicate in neurons and how they affect their physiology. This study compares the entry and replication in neurons and the functional consequences on the neural network upon infection with an old “historical” strain and contemporary “circulating” strains of EV-D68.
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- 2023
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8. A Response to: Letter to the Editor Regarding “Neurophysiological Assessments During Continuous Sedation Until Death Put Validity of Observational Assessments Into Question: A Prospective Observational Study”
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Six, Stefaan, Poelaert, Jan, Laureys, Steven, Maîresse, Olivier, Theuns, Peter, Bilsen, Johan, and Deschepper, Reginald
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- 2022
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9. COVID-19 and Cushing’s disease in a patient with ACTH-secreting pituitary carcinoma
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J M K de Filette, Bastiaan Sol, Gil Awada, Corina E Andreescu, David Unuane, Sandrine Aspeslagh, Jan Poelaert, and Bert Bravenboer
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Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
The pandemic caused by severe acute respiratory syndrome coronavirus 2 is of an unprecedented magnitude and has made it challenging to properly treat patients with urgent or rare endocrine disorders. Little is known about the risk of coronavirus disease 2019 (COVID-19) in patients with rare endocrine malignancies, such as pituitary carcinoma. We describe the case of a 43-year-old patient with adrenocorticotrophic hormone-secreting pituitary carcinoma who developed a severe COVID-19 infection. He had stabilized Cushing’s disease after multiple lines of treatment and was currently receiving maintenance immunotherapy with nivolumab (240 mg every 2 weeks) and steroidogenesis inhibition with ketoconazole (800 mg daily). On admission, he was urgently intubated for respiratory exhaustion. Supplementation of corticosteroid requirements consisted of high-dose dexamethasone, in analogy with the RECOVERY trial, followed by the reintroduction of ketoconazole under the coverage of a hydrocortisone stress regimen, which was continued at a dose depending on the current level of stress. He had a prolonged and complicated stay at the intensive care unit but was eventually discharged and able to continue his rehabilitation. The case points out that multiple risk factors for severe COVID-19 are present in patients with Cushing’s syndrome. ‘Block-replacement’ therapy with suppression of endogenous steroidogenesis and supplementation of corticosteroid requirements might be preferred in this patient population.
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- 2022
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10. The pathophysiological impact of intra-abdominal hypertension in pigs.
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Robert Wise, Reitze Rodseth, Ester Párraga-Ros, Rafael Latorre, Octavio López Albors, Laura Correa-Martín, Francisco M Sánchez-Margallo, Irma Eugenia Candanosa-Aranda, Jan Poelaert, Gregorio Castellanos, and Manu L N G Malbrain
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Medicine ,Science - Abstract
BackgroundIntra-abdominal hypertension and abdominal compartment syndrome are common with clinically significant consequences. We investigated the pathophysiological effects of raised IAP as part of a more extensive exploratory animal study. The study design included both pneumoperitoneum and mechanical intestinal obstruction models.MethodsForty-nine female swine were divided into six groups: a control group (Cr; n = 5), three pneumoperitoneum groups with IAPs of 20mmHg (Pn20; n = 10), 30mmHg (Pn30; n = 10), 40mmHg (Pn40; n = 10), and two mechanical intestinal occlusion groups with IAPs of 20mmHg (MIO20; n = 9) and 30mmHg (MIO30; n = 5).ResultsThere were significant changes (pConclusionsThis study identified and quantified the impact of intra-abdominal hypertension at different pressures on several organ systems and highlighted the significance of even short-lived elevations. Two models of intra-abdominal pressure were used, with a mechanical obstruction model showing more rapid changes in metabolic and haematological changes. These may represent different underlying cellular and vascular pathophysiological processes, but this remains unclear.
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- 2023
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11. Publisher Correction: The Value of Rotavirus Vaccination in Europe: A Call for Action
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Cohen, Robert, Martinón-Torres, Federico, Posiuniene, Inga, Benninghoff, Bernd, Oh, Kyu-Bin, and Poelaert, Dirk
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- 2023
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12. The Role of Cytoreductive Radical Prostatectomy in the Treatment of Newly Diagnosed Low-volume Metastatic Prostate Cancer. Results from the Local Treatment of Metastatic Prostate Cancer (LoMP) Registry
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Lumen, Nicolaas, De Bleser, Elise, Buelens, Sarah, Verla, Wesley, Poelaert, Filip, Claeys, Wietse, Fonteyne, Valérie, Verbeke, Sofie, Villeirs, Geert, De Man, Kathia, Rottey, Sylvie, Van Praet, Charles, Decaestecker, Karel, and Ost, Piet
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- 2021
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13. The Role of Cytoreductive Radical Prostatectomy in the Treatment of Newly Diagnosed Low-volume Metastatic Prostate Cancer. Results from the Local Treatment of Metastatic Prostate Cancer (LoMP) Registry
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Nicolaas Lumen, Elise De Bleser, Sarah Buelens, Wesley Verla, Filip Poelaert, Wietse Claeys, Valérie Fonteyne, Sofie Verbeke, Geert Villeirs, Kathia De Man, Sylvie Rottey, Charles Van Praet, Karel Decaestecker, and Piet Ost
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Prostate cancer ,Metastasis ,Radical prostatectomy ,Prostate ,Androgen deprivation therapy ,Diseases of the genitourinary system. Urology ,RC870-923 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background: Radiotherapy to the prostate (RTp) prolongs survival for patients with low-volume, newly diagnosed metastatic prostate cancer (ndmPC). Objective: to evaluate whether cytoreductive radical prostatectomy (cRP) is equally beneficial as RTp in low-volume ndmPC. Design, setting, and participants: A multicenter prospective registry was established in 2014 to observe patients with ndmPC. Eligible patients were offered cRP or RTp. For this study we selected only patients with low-volume ndmPC (n = 109). Of these, 48, 26, and 35 patients underwent cRP, RTp, and no local therapy (NLT), respectively. Median follow-up was 32 mo (interquartile range 16–49). Intervention: cRP was compared with RTp and NLT. Outcome measurements and statistical analysis: Overall survival (OS), cancer-specific survival (CSS), and local event–free survival (LEFS) were calculated using the Kaplan-Meier method. Factors prognostic for OS were identified using univariate and multivariate Cox regression analysis. Results and limitations: The 2-yr OS was 93%, 100%, and 69%, and 2-yr CSS was 93%, 100%, and 75% for cRP, RTp, and NLT, respectively. The cRP and RTp groups had better OS compared to NLT and there was no significant difference between cRP and RTp. The 2-yr LEFS was 92%, 77%, and 60% for cRP, RTp, and NLT, respectively. The cRP group had better LEFS compared to RTp and NLT, and there was no significant difference between RTp and NLT. Advanced tumor stage, Eastern Cooperative Oncology Group performance status ≥2, and NLT were negative prognostic factors for OS. The main limitation is selection of fitter patients with less advanced tumors for cRP and the small sample size. Conclusions: For selected patients with low-volume ndmPC, cRP is able to achieve similar OS and CSS to RTp. cRP is effective in preventing local events due to disease progression. Patient summary: Patients with a low volume of newly diagnosed prostate cancer that has spread beyond the prostate gland might benefit from removal of the prostate, which we found was as effective as radiotherapy to the prostate in prolonging survival. Removal of the prostate is effective in preventing urinary problems caused by cancer progression.
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- 2021
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14. Neurophysiological Assessments During Continuous Sedation Until Death Put Validity of Observational Assessments Into Question: A Prospective Observational Study
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Six, Stefaan, Laureys, Steven, Poelaert, Jan, Maîresse, Olivier, Theuns, Peter, Bilsen, Johan, and Deschepper, Reginald
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- 2021
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15. Forty years of neuromuscular monitoring and postoperative residual curarisation: a meta-analysis and evaluation of confidence in network meta-analysis
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Carvalho, Hugo, Verdonck, Michael, Cools, Wilfried, Geerts, Lieselot, Forget, Patrice, and Poelaert, Jan
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- 2020
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16. Attitudes of Professional Caregivers and Family Members Regarding the Use of Monitoring Devices to Improve Assessments of Pain and Discomfort During Continuous Sedation Until Death
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Six, Stefaan, Van Overmeire, Roel, Bilsen, Johan, Laureys, Steven, Poelaert, Jan, Theuns, Peter, and Deschepper, Reginald
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- 2020
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17. Multi-organ point-of-care ultrasound for COVID-19 (PoCUS4COVID): international expert consensus
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Arif Hussain, Gabriele Via, Lawrence Melniker, Alberto Goffi, Guido Tavazzi, Luca Neri, Tomas Villen, Richard Hoppmann, Francesco Mojoli, Vicki Noble, Laurent Zieleskiewicz, Pablo Blanco, Irene W. Y. Ma, Mahathar Abd. Wahab, Abdulmohsen Alsaawi, Majid Al Salamah, Martin Balik, Diego Barca, Karim Bendjelid, Belaid Bouhemad, Pablo Bravo-Figueroa, Raoul Breitkreutz, Juan Calderon, Jim Connolly, Roberto Copetti, Francesco Corradi, Anthony J. Dean, André Denault, Deepak Govil, Carmela Graci, Young-Rock Ha, Laura Hurtado, Toru Kameda, Michael Lanspa, Christian B. Laursen, Francis Lee, Rachel Liu, Massimiliano Meineri, Miguel Montorfano, Peiman Nazerian, Bret P. Nelson, Aleksandar N. Neskovic, Ramon Nogue, Adi Osman, José Pazeli, Elmo Pereira-Junior, Tomislav Petrovic, Emanuele Pivetta, Jan Poelaert, Susanna Price, Gregor Prosen, Shalim Rodriguez, Philippe Rola, Colin Royse, Yale Tung Chen, Mike Wells, Adrian Wong, Wang Xiaoting, Wang Zhen, and Yaseen Arabi
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COVID-19 ,SARS-CoV-2 ,Point-of-care ultrasound (PoCUS) ,Focused cardiac ultrasound (FoCUS) ,Lung ultrasound (LUS) ,Echocardiography ,Medical emergencies. Critical care. Intensive care. First aid ,RC86-88.9 - Abstract
Abstract COVID-19 has caused great devastation in the past year. Multi-organ point-of-care ultrasound (PoCUS) including lung ultrasound (LUS) and focused cardiac ultrasound (FoCUS) as a clinical adjunct has played a significant role in triaging, diagnosis and medical management of COVID-19 patients. The expert panel from 27 countries and 6 continents with considerable experience of direct application of PoCUS on COVID-19 patients presents evidence-based consensus using GRADE methodology for the quality of evidence and an expedited, modified-Delphi process for the strength of expert consensus. The use of ultrasound is suggested in many clinical situations related to respiratory, cardiovascular and thromboembolic aspects of COVID-19, comparing well with other imaging modalities. The limitations due to insufficient data are highlighted as opportunities for future research.
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- 2020
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18. Acceptance of mHealth among health professionals: a case study on anesthesia practitioners
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Hugo Carvalho, Michael Verdonck, Patrice Forget, and Jan Poelaert
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Anesthesia ,mHealth ,Smartphone application ,Smartphone peripherals ,Apps ,Anesthesiology ,RD78.3-87.3 - Abstract
Abstract Background mHealth, the practice of medicine aided by mobile devices is a growing market. Although the offer on Anesthesia applications (Apps) is quite prolific, representative formal assessments on the views of anesthesia practitioners on its use and potential place in daily practice is lacking. This survey aimed thus to cross-assess the Belgian anesthesia population on the use of smartphone Apps and peripherals. Methods The survey was exclusively distributed as an online anonymous questionnaire. Sharing took place via hyperlink forwarding by the Belgian Society for Anesthesia and Reanimation (BSAR) and by the Belgian Association for Regional Anesthesia (BARA) to all registered members. The first answer took place on 5 September 2018, the last on 22 January 2019. Results Three hundred forty-nine answers were obtained (26.9% corresponding to trainees, 73.1% to specialists). Anesthesiologists were positively confident that Apps and peripherals could help improve anesthesia care (57.0 and 47.9%, respectively, scored 4 or 5, in a scale from 0 to 5). Trainees were significantly more confident than specialists on both mobile Apps (71.2% and 51.8%, respectively; p = 0.001) and peripherals (77.7% and 45.1%, respectively; p = 0.09). The usefulness of Apps and Peripherals was rated 1 or below (on a 0 to 5 scale), respectively, by 9.5 and 14.6% of the total surveyed population, being specialists proportionally less confident in Smartphone peripherals than trainees (p = 0.008). Mobile apps are actively used by a significantly higher proportional number of trainees (67.0% vs. 37.3%, respectively; p = 0.000001). The preferred category of mobile Apps was dose-calculating applications (39.15%), followed by digital books (21. 1%) and Apps for active perioperative monitoring (20.0%). Conclusions Belgian Anesthesia practitioners show a global positive attitude towards smartphone Apps and Peripherals, with trainees trending to be more confident than specialists. Trial registration ClinicalTrials.gov database Identifier: NCT03750084 . Retrospectively registered on 21 November 2018.
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- 2020
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19. The histone deacetylase inhibitor M344 as a multifaceted therapy for pancreatic cancer
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Shelby M. Knoche, Gabrielle L. Brumfield, Benjamin T. Goetz, Bailee H. Sliker, Alaina C. Larson, Madeline T. Olson, Brittany J. Poelaert, Audrey Bavari, Ying Yan, Jennifer D. Black, and Joyce C. Solheim
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Medicine ,Science - Abstract
The histone deacetylase (HDAC) inhibitor vorinostat, used with gemcitabine and other therapies, has been effective in treatment of experimental models of pancreatic cancer. In this study, we demonstrated that M344, an HDAC inhibitor, is efficacious against pancreatic cancer in vitro and in vivo, alone or with gemcitabine. By 24 hours post-treatment, M344 augments the population of pancreatic cancer cells in G1, and at a later time point (48 hours) it increases apoptosis. M344 inhibits histone H3 deacetylation and slows pancreatic cancer cell proliferation better than vorinostat, and it does not decrease the viability of a non-malignant cell line more than vorinostat. M344 also elevates pancreatic cancer cell major histocompatibility complex (MHC) class I molecule expression, potentially increasing the susceptibility of pancreatic cancer cells to T cell lysis. Taken together, our findings support further investigation of M344 as a pancreatic cancer treatment.
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- 2022
20. Association between night-time surgery and occurrence of intraoperative adverse events and postoperative pulmonary complications
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Kroell, W., Metzler, H., Struber, G., Wegscheider, T., Gombotz, H., Hiesmayr, M., Schmid, W., Urbanek, B., Kahn, D., Momeni, M., Pospiech, A., Lois, F., Forget, P., Grosu, I., Poelaert, J., Mossevelde, V., van Malderen, M.C., Dylst, D., Melkebeek, J.V., Beran, M., Hert, S.D., Baerdemaeker, L.D., Heyse, B., Limmen, J.V., Wyffels, P., Jacobs, T., Roels, N., Bruyne, A.D., Velde, S.V.D., Marina, J.Z., Dejana, D.O., Pernar, S., Zunic, J., Miskovic, P., Zilic, A., Kvolik, S., Ivic, D., Darija, A.V., Skiljic, S., Vinkovic, H., Oputric, I., Juricic, K., Frkovic, V., Kopic, J., Mirkovic, I., Saric, J.P., Erceg, G., Dvorscak, M.B., Branka, M.S., Pavicic, A.M., Goranovic, T., Maldini, B., Radocaj, T., Gavranovic, Z., Inga, M.B., Sehovic, M., Stourac, P., Harazim, H., Smekalova, O., Kosinova, M., Kolacek, T., Hudacek, K., Drab, M., Brujevic, J., Vitkova, K., Jirmanova, K., Volfova, I., Dzurnakova, P., Liskova, K., Dudas, R., Filipsky, R., Kafrawy, S.E., Abdelwahab, H.H., Metwally, T., Ahmed, A.R., Ahmed Mostafa, E.S., Hasan, W.F., Ahmed, A.G., Yassin, H., Magdy, M., Abdelhady, M., Mahran, M., Herodes, E., Kivik, P., Oganjan, J., Aun, A., Sormus, A., Sarapuu, K., Mall, M., Karjagin, J., Futier, E., Petit, A., Gerard, A., Marret, E., Solier, M., Jaber, S., Prades, A., Krassler, J., Merzky, S., Abreu, M.G.D., Uhlig, C., Kiss, T., Bundy, A., Bluth, T., Gueldner, A., Spieth, P., Scharffenberg, M., Thiem, D.T., Koch, T., Treschan, T., Schaefer, M., Bastin, B., Geib, J., Weiss, M., Kienbaum, P., Pannen, B., Gottschalk, A., Konrad, M., Westerheide, D., Schwerdtfeger, B., Wrigge, H., Simon, P., Reske, A., Nestler, C., Valsamidis, D., Stroumpoulis, K., Antholopoulos, G., Andreou, A., Karapanos, D., Theodoraki, K., Gkiokas, G., Tasoulis, M.K., Sidiropoulou, T., Zafeiropoulou, F., Florou, P., Pandazi, A., Tsaousi, G., Nouris, C., Pourzitaki, C., Bystritski, D., Pizov, R., Eden, A., Pesce, C.V., Campanile, A., Marrella, A., Grasso, S., Michele, M.D., Bona, F., Giacoletto, G., Sardo, E., Sottosanti, L.G.V., Solca, M., Volta, C.A., Spadaro, S., Verri, M., Ragazzi, R., Zoppellari, R., Cinnella, G., Raimondo, P., Bella, D.L., Mirabella, L., D'antini, D., Pelosi, P., Molin, A., Brunetti, I., Gratarola, A., Pellerano, G., Sileo, R., Pezzatto, S., Montagnani, L., Pasin, L., Landoni, G., Zangrillo, A., Beretta, L., Parma, A.L.D., Tarzia, V., Dossi, R., Sassone, M.E., Sances, D., Tredici, S., Spano, G., Castellani, G., Delunas, L., Peradze, S., Venturino, M., Arpino, I., Sher, S., Tommasino, C., Rapido, F., Morelli, P., Vargas, M., Servillo, G., Cortegiani, A., Raineri, S.M., Montalto, F., Russotto, V., Giarratano, A., Baciarello, M., Generali, M., Cerati, G., Leykin, Y., Bressan, F., Bartolini, V., Zamidei, L., Brazzi, L., Liperi, C., Sales, G., Pistidda, L., Severgnini, P., Brugnoni, E., Musella, G., Bacuzzi, A., Muhardri, D., Agreta, G.G., Sada, F., Bytyqi, A., Karbonskiene, A., Aukstakalniene, R., Teberaite, Z., Salciute, E., Tikuisis, R., Miliauskas, P., Jurate, S., Kontrimaviciute, E., Tomkute, G., Xuereb, J., Bezzina, M., Borg, F. Joseph, Hemmes, S., Schultz, M., Hollmann, M., Wiersma, I., Binnekade, J., Bos, L., Boer, C., Duvekot, A., Veld, B.I ‘t, Werger, A., Dennesen, P., Severijns, C., Jong, J.D., Hering, J., Beek, R.V., Ivars, S., Jammer, I.B., Breidablik, A., Hodt, K.S., Fjellanger, F., Avalos, M.V., Jannicke, M.O., Andersson, E., Amir, S.K., Molina, R., Wutai, S., Morais, E., Tareco, G., Ferreira, D., Amaral, J., Castro, M.D.L.G., Cadilha, S., Appleton, S., Parente, S., Correia, M., Martins, D., Monteirosa, A., Ricardo, A., Rodrigues, S., Horhota, L., Grintescu, I.M., Mirea, L., Grintescu, I.C., Corneci, D., Negoita, S., Dutu, M., Popescu Garotescu, I., Filipescu, D., Prodan, A.B., Droc, G., Fota, R., Popescu, M., Tomescu, D., Petcu, A.M., Tudoroiu, M.I., Moise, A., Guran, C.T., Gherghina, I., Costea, D., Cindea, I., Copotoiu, S.M., Copotoiu, R., Barsan, V., Tolcser, Z., Riciu, M., Moldovan, S.G., Veres, M., Gritsan, A., Kapkan, T., Gritsan, G., Korolkov, O., Kulikov, A., Lubnin, A., Ovezov, A., Prokoshev, P., Lugovoy, A., Anipchenko, N., Babayants, A., Komissarova, I., Zalina, K., Likhvantsev, V., Fedorov, S., Lazukic, A., Pejakovic, J., Mihajlovic, D., Kusnierikova, Z., Zelinkova, M., Bruncakova, K., Polakovicova, L., Sobona, V., Barbka, N.S., Ana, P.G., Jovanov, M., Strazisar, B., Jasmina, M.B., Vesna, N.J., Voje, M., Grynyuk, A., Kostadinov, I., Alenka, S.V., Moral, V., Unzueta, M.C., Puigbo, C., Fava, J., Canet, J., Moret, E., Nunez, M.R., Sendra, M., Brunelli, A., Rodenas, F., Monedero, P., Hidalgo, F., Yepes, M.J., Martinez-Simon, A., Abajo, A., Lisi, A., Perez, G., Martinez, R., Granell, M., Vivo, J.T., Ruiz, C.S., Andrés Ibañez, J.A.D., Pastor, E., Soro, M., Ferrando, C., Defez, M., Cesar Aldecoa, A.S., Perez, R., Rico, J., Jawad, M., Saeed, Y., Gillberg, L., Bengisun, Z.K., Kazbek, B.K., Coskunfirat, N., Boztug, N., Sanli, S., Yilmaz, M., Hadimioglu, N., Senturk, N.M., Camci, E., Kucukgoncu, S., Sungur, Z., Sivrikoz, N., Ozgen, S.U., Toraman, F., Selvi, O., Senturk, O., Yildiz, M., Kuvaki, B., Gunenc, F., Kucukguclu, S., Ozbilgin, S., Maral, J., Canli, S., Arun, O., Saltali, A., Aydogan, E., Akgun, F.N., Sanlikarip, C., Karaman, F.M., Mazur, A., Vorotyntsev, S., Rousseau, G., Barrett, C., Stancombe, L., Shelley, B., Scholes, H., Limb, J., Rafi, A., Wayman, L., Deane, J., Rogerson, D., Williams, J., Yates, S., Rogers, E., Pulletz, M., Moreton, S., Jones, S., Venkatesh, S., Burton, M., Brown, L., Goodall, C., Rucklidge, M., Fuller, D., Nadolski, M., Kusre, S., Lundberg, M., Everett, L., Nutt, H., Zuleika, M., Carvalho, P., Clements, D., Ben, C.B., Watt, P., Raymode, P., Pearse, R., Mohr, O., Raj, A., Creary, T., Chishti, A., Bell, A., Higham, C., Cain, A., Gibb, S., Mowat, S., Franklin, D., West, C., Minto, G., Boyd, N., Mills, G., Calton, E., Walker, R., Mackenzie, F., Ellison, B., Roberts, H., Chikungwa, M., Jackson, C., Donovan, A., Foot, J., Homan, E., Montgomery, J., Portch, D., Mercer, P., Palmer, J., Paddle, J., Fouracres, A., Datson, A., Andrew, A., Welch, L., Rose, A., Varma, S., Simeson, K., Rambhatla, M., Susarla, J., Marri, S., Kodaganallur, K., Das, A., Algarsamy, S., Colley, J., Davies, S., Szewczyk, M., Smith, T., Ana, F.B., Luzier, E., Almagro, A., Melo, M.V., Fernando, L., Sulemanji, D., Sprung, J., Weingarten, T., Kor, D., Scavonetto, F., Tze, Y., Gregoretti, C., Neto, A.S., Hemmes, S.N.T., Ball, L., Hollmann, M.W., Mills, G.H., Melo, M.F.V., Putensen, C., Gama de Abreu, M., and Schultz, M.J.
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- 2019
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21. Surface Area and Curvature of the general Ellipsoid
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Poelaert, Daniel, Schniewind, Joachim, and Janssens, Frank
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Mathematics - Classical Analysis and ODEs - Abstract
This paper gives a detailed derivation of the surface of a tri-axial ellipsoid. The general result is in terms of the elliptic integrals of the first and second kind. It is in checked for all special cases included and the corresponding simplified formulae are given. Next, expressions for the mean and Gaussian curvature are derived. These curvatures depend only on: a) the three axes, and from the point under consideration, b) its distance to the centre and c) the length of the perpendicular from centre to the tangent plane of that point. Finally, it is noticed that at the four umbilic points of an ellipsoid, where the cicular sections degenerate to a point, the two principal curvatures are equal and have the simple expression (a c / b^3)where a>b>c are the half-axes., Comment: 12 pages, 3 figures
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- 2011
22. Superficial Serratus Anterior Plane Block for Minimal Invasive Cardiac Surgery: a single-center randomized controlled trial.
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VAES, B., VAN HOECKE, L., ALLAERT, S., MAES, J.-W., FRANÇOIS, J., POELAERT, J., and LAPAGE, K.
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- 2024
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23. Re: Effect of intrapartum epidural analgesia on rate of emergency delivery for presumed fetal compromise: nationwide registry‐based cohort study
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Verheggen, A. F. O., primary, Lapage, K., additional, and Poelaert, J., additional
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- 2023
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24. Contemporary perioperative haemodynamic monitoring
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Qianyun Pang, Jan Hendrickx, Hong-Liang Liu, and Jan Poelaert
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haemodynamics ,monitoring ,noninvasive ,Anesthesiology ,RD78.3-87.3 ,Medical emergencies. Critical care. Intensive care. First aid ,RC86-88.9 - Abstract
Haemodynamic monitoring is the cornerstone in the optimization of tissue perfusion and the prevention of deteriorating metabolism. Haemodynamic alterations could be summarized in terms of cardiac dysfunction, changes of loading conditions (preload or/and afterload), and patient related issues. This review aims to present the clinical applications of different haemodynamic monitoring techniques, discuss advantages and disadvantages, and provide guidance to help the clinician select those techniques suitable to optimize haemodynamics in individual patients during the perioperative period.
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- 2019
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25. Unravelling the first key steps in equine herpesvirus type 5 (EHV5) pathogenesis using ex vivo and in vitro equine models
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Jolien Van Cleemput, Katrien C. K. Poelaert, Kathlyn Laval, and Hans J. Nauwynck
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Veterinary medicine ,SF600-1100 - Abstract
Abstract Equine herpesvirus type 5 (EHV5) is a ubiquitous, yet obscure pathogen in the horse population and is commonly associated with fatal equine multinodular pulmonary fibrosis (EMPF). To date, little is known about the precise pathogenesis of EHV5. Here, we evaluated the dynamics of EHV5 infection in representative ex vivo and in vitro equine models, using immunofluorescence staining and virus titration. EHV5 was unable to infect epithelial cells lining the mucosa of nasal and tracheal explants. Similarly, primary equine respiratory epithelial cells (EREC) were not susceptible to EHV5 following inoculation at the apical or basolateral surfaces. Upon direct delivery of EHV5 particles to lung explants, few EHV5-positive cell clusters were observed at 72 hours post-inoculation (hpi). These EHV5-positive cells were identified as cytokeratin-positive alveolar cells. Next, we examined the potential of EHV5 to infect three distinct equine PBMC populations (CD172a+ monocytes, CD3+ T lymphocytes and Ig light chain+ B lymphocytes). Monocytes did not support EHV5 replication. In contrast, up to 10% of inoculated equine T and B lymphocytes synthetized intracellular viral antigens 24 hpi and 72 hpi, respectively. Still, the production of mature virus particles was hampered, as we did not observe an increase in extracellular virus titer. After reaching a peak, the percentage of infected T and B lymphocytes decayed, which was partly due to the onset of apoptosis, but not necrosis. Based on these findings, we propose a model for EHV5 pathogenesis in the horse. Uncovering EHV5 pathogenesis is the corner step to finally contain or even eradicate the virus.
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- 2019
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26. Exploratory Outlier Detection for Acceleromyographic Neuromuscular Monitoring: Machine Learning Approach
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Michaël Verdonck, Hugo Carvalho, Johan Berghmans, Patrice Forget, and Jan Poelaert
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Computer applications to medicine. Medical informatics ,R858-859.7 ,Public aspects of medicine ,RA1-1270 - Abstract
BackgroundBecause of their kinetic nature, artifactual recordings of acceleromyography-based neuromuscular monitoring devices are not unusual. These generate a great deal of cynicism among anesthesiologists, constituting an obstacle toward their widespread adoption. Through outlier analysis techniques, monitoring devices can learn to detect and flag signal abnormalities. ObjectiveThis study aims to engineer a set of features that enable the detection of outliers in the form of erroneous train-of-four (TOF) measurements from an acceleromyographic-based device. These features are tested for their potential in the detection of erroneous TOF measurements by developing an outlier detection algorithm. MethodsA data set encompassing 533 high-sensitivity TOF measurements from 35 patients was created based on a multicentric open label trial of a purpose-built accelero- and gyroscopic-based neuromuscular monitoring app. A basic set of features was extracted based on raw data while a second set of features was purpose engineered based on TOF pattern characteristics. Two cost-sensitive logistic regression (CSLR) models were deployed to evaluate the performance of these features. The final output of the developed models was a binary classification, indicating if a TOF measurement was an outlier or not. ResultsA total of 7 basic features were extracted based on raw data, while another 8 features were engineered based on TOF pattern characteristics. The model training and testing were based on separate data sets: one with 319 measurements (18 outliers) and a second with 214 measurements (12 outliers). The F1 score (95% CI) was 0.86 (0.48-0.97) for the CSLR model with engineered features, significantly larger than the CSLR model with the basic features (0.29 [0.17-0.53]; P
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- 2021
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27. Noncanonical Transmission of a Measles Virus Vaccine Strain from Neurons to Astrocytes
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Katrien C. K. Poelaert, Riley M. Williams, Christine M. Matullo, and Glenn F. Rall
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Microbiology ,QR1-502 - Abstract
Viruses are the most important cause of infectious encephalitis in mammals worldwide; several thousand people, primarily the very young and the elderly, are impacted annually, and few therapies are reliably successful once neuroinvasion has occurred. To understand how viruses contribute to neuropathology, and to develop tools to prevent or ameliorate such infections, it is crucial to define if and how viruses disseminate among the different cell populations within the highly complex central nervous system.
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- 2021
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28. The Pathogenesis and Immune Evasive Mechanisms of Equine Herpesvirus Type 1
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Kathlyn Laval, Katrien C. K. Poelaert, Jolien Van Cleemput, Jing Zhao, Annelies P. Vandekerckhove, Annick C. Gryspeerdt, Barbara Garré, Karen van der Meulen, Hossein B. Baghi, Haileleul N. Dubale, Ines Zarak, Eline Van Crombrugge, and Hans J. Nauwynck
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pathogenesis ,immune evasion ,prevention ,therapies ,Equine Herpesvirus type 1 ,Microbiology ,QR1-502 - Abstract
Equine herpesvirus type 1 (EHV-1) is an alphaherpesvirus related to pseudorabies virus (PRV) and varicella-zoster virus (VZV). This virus is one of the major pathogens affecting horses worldwide. EHV-1 is responsible for respiratory disorders, abortion, neonatal foal death and equine herpes myeloencephalopathy (EHM). Over the last decade, EHV-1 has received growing attention due to the frequent outbreaks of abortions and/or EHM causing serious economical losses to the horse industry worldwide. To date, there are no effective antiviral drugs and current vaccines do not provide full protection against EHV-1-associated diseases. Therefore, there is an urgent need to gain a better understanding of the pathogenesis of EHV-1 in order to develop effective therapies. The main objective of this review is to provide state-of-the-art information on the pathogenesis of EHV-1. We also highlight recent findings on EHV-1 immune evasive strategies at the level of the upper respiratory tract, blood circulation and endothelium of target organs allowing the virus to disseminate undetected in the host. Finally, we discuss novel approaches for drug development based on our current knowledge of the pathogenesis of EHV-1.
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- 2021
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29. Reducing agent can be omitted in the incubation medium of the batch in vitro fermentation model of the pig intestines
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Poelaert, C., Nollevaux, G., Boudry, C., Taminiau, B., Nezer, C., Daube, G., Schneider, Y.-J., Portetelle, D., Théwis, A., and Bindelle, J.
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- 2018
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30. The surgical safety checklist and patient outcomes after surgery: a prospective observational cohort study, systematic review and meta-analysis
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Pearse, Rupert M., Beattie, Scott, Clavien, Pierre-Alain, Demartines, Nicolas, Fleisher, Lee A., Grocott, Mike, Haddow, James, Hoeft, Andreas, Holt, Peter, Moreno, Rui, Pritchard, Naomi, Rhodes, Andrew, Wijeysundera, Duminda, Wilson, Matt, Ahmed, Tahania, Everingham, Kirsty, Hewson, Russell, Januszewska, Marta, Phull, Mandeep-Kaur, Halliwell, Richard, Shulman, Mark, Myles, Paul, Schmid, Werner, Hiesmayr, Michael, Wouters, Patrick, de Hert, Stefan, Lobo, Suzana, Fang, Xiangming, Rasmussen, Lars, Futier, Emmanuel, Biais, Matthieu, Venara, Aurélien, Slim, Karem, Sander, Michael, Koulenti, Despoina, Arvaniti, Kostoula, Chan, Mathew, Kulkarni, Atul, Chandra, Susilo, Tantri, Aida, Geddoa, Emad, Abbas, Muntadhar, Della Rocca, Giorgio, Sivasakthi, Datin, Mansor, Marzida, Luna, Pastor, Bouwman, Arthur, Buhre, Wolfgang, Beavis, Vanessa, Campbell, Douglas, Short, Tim, Osinaike, Tunde, Matos, Ricardo, Grigoras, Ioana, Kirov, Mikhail, Protsenko, Denis, Biccard, Bruce, Aldecoa, Cesar, Chew, Michelle, Hofer, Christoph, Hubner, Martin, Ditai, James, Szakmany, Tamas, Fleisher, Lee, Ferguson, Marissa, MacMahon, Michael, Cherian, Ritchie, Currow, Helen, Kanathiban, Kathirgamanathan, Gillespie, David, Pathmanathan, Edward, Phillips, Katherine, Reynolds, Jenifer, Rowley, Joanne, Douglas, Jeanene, Kerridge, Ross, Garg, Sameer, Bennett, Michael, Jain, Megha, Alcock, David, Terblanche, Nico, Cotter, Rochelle, Leslie, Kate, Stewart, Marcelle, Zingerle, Nicolette, Clyde, Antony, Hambidge, Oliver, Rehak, Adam, Cotterell, Sharon, Huynh, Wilson Binh Quan, McCulloch, Timothy, Ben-Menachem, Erez, Egan, Thomas, Cope, Jennifer, Fellinger, Paul, Haisjackl, Markus, Haselberger, Simone, Holaubek, Caroline, Lichtenegger, Paul, Scherz, Florian, Hoffer, Franz, Cakova, Veronika, Eichwalder, Andreas, Fischbach, Norbert, Klug, Reinhold, Schneider, Elisabeth, Vesely, Martin, Wickenhauser, Reinhart, Grubmueller, Karl Gernot, Leitgeb, Marion, Lang, Friedrich, Toro, Nancy, Bauer, Marlene, Laengle, Friedrich, Haberl, Claudia, Mayrhofer, Thomas, Trybus, Christoph, Buerkle, Christian, Forstner, Karin, Germann, Reinhard, Rinoesl, Harald, Schindler, Elke, Trampitsch, Ernst, Bogner, Gerhard, Dankl, Daniel, Duenser, Martin, Fritsch, Gerhard, Gradwohl-Matis, Ilse, Hartmann, Andreas, Hoelzenbein, Thomas, Jaeger, Tarkan, Landauer, Franz, Lindl, Gregor, Lux, Michael, Steindl, Johannes, Stundner, Ottokar, Szabo, Christian, Bidgoli, Jawad, Verdoodt, Hans, Forget, Patrice, Kahn, David, Lois, Fernande, Momeni, Mona, Prégardien, Caroline, Pospiech, Audrey, Steyaert, Arnaud, Veevaete, Laurent, De Kegel, Dirk, De Jongh, Karen, Foubert, Luc, Smitz, Carine, Vercauteren, Marcel, Poelaert, Jan, Van Mossevelde, Veerle, Abeloos, Jacques, Bouchez, Stefaan, Coppens, Marc, De Baerdemaeker, Luc, Deblaere, Isabel, De Bruyne, Ann, Fonck, Kristine, Heyse, Bjorn, Jacobs, Tom, Lapage, Koen, Moerman, Anneliese, Neckebroek, Martine, Parashchanka, Aliaksandra, Roels, Nathalie, Van Den Eynde, Nancy, Vandenheuvel, Michael, Limmen, JurgenVan, Vanluchene, Ann, Vanpeteghem, Caroline, Wyffels, Piet, Huygens, Christel, Vandenbempt, Punitha, Van de Velde, Marc, Dylst, Dimitri, Janssen, Bruno, Schreurs, Evelien, Aleixo, Fábia Berganton, Candido, Keulle, Batista, Hugo Dias, Guimarães, Mario, Guizeline, Jaqueline, Hoffmann, João, Lobo, Francisco Ricardo Marques, Nascimento, Vinícius, Nishiyama, Katia, Pazetto, Lucas, Souza, Daniela, Rodrigues, Rodrigo Souza, Vilela dos Santos, Ana Maria, Jardim, Jaquelline, Sá Malbouisson, Luiz Marcelo, Silva, Joao, Nascimento Junior, Paulo do, Baio, Thalissa Hermínia, Pereira de Castro, Gabriel Isaac, Watanabe Oliveira, Henri Roger, Amendola, Cristina Prata, Cardoso, Gutemberg, Ortega, Daniela, Brotto, Ana Flavia, De Oliveira, Mirella Cristine, Réa-Neto, Álvaro, Dias, Fernando, Travi, Maria Eduarda, Zerman, Luiza, Azambuja, Pedro, Knibel, Marcos Freitas, Martins, Antonio, Almeida, William, Neto, Calim Neder, Tardelli, Maria Angela, Caser, Eliana, Machado, Marcio, Aguzzoli, Crisitiano, Baldisserotto, Sérgio, Tabajara, Fernanda Beck, Bettega, Fernanda, Rodrigues Júnior, La Hore Correa, de Gasperi, Julia, Faina, Lais, Nolasco, Marcos Farias, Ferreira da Costa Fischer, Bruna, Fosch de Campos Ferreira, Mariana, Hartmann, Cristina, Kliemann, Marta, Hubert Ribeiro, Gustavo Luis, Fraga, Julia Merladete, Netto, Thiago Motta, Pozza, Laura Valduga, Wendling, Paulo Rafael, Azevedo, Caroline, Garcia, Juliana, Lopes, Marcel, Maia, Bernardo, Maselli, Paula, Melo, Ralph, Mendes, Weslley, Neves, Matheus, Ney, Jacqueline, Piras, Claudio, Applewhaite, Christopher, Carr, Adrienne, Chow, Lorraine, Duttchen, Kaylene, Foglia, Julena, Greene, Michael, Hinther, Ashley, Houston, Kendra, McCormick, Thomas Jared, Mikhayel, Jennifer, Montasser, Sam, Ragan, Alex, Suen, Andrew, Woolsey, Adrianna, Yu, Hai Chuan, Funk, Duane, Kowalski, Stephen, Legaspi, Regina, McDonald, Heather, Siddiqui, Faisal, Pridham, Jeremy, Rowe, Bernadette, Sampson, Sonia, Thiessen, Barton, Zbitnew, Geoff, Bernard, Andre, George, Ronald, Jones, Philip, Moor, Rita, Siddiqui, Naveed, Wolfer, Alexandra, Tran, Diem, Winch, Denyse, Dobson, Gary, McCormick, Thomas, Montasser, Osama, Hall, Richard, Baghirzada, Leyla, Curley, Gerard, Dai, Si Yuan, Hare, Gregory, Lee, Esther, Shastri, Uma, Tsui, Albert, Yagnik, Anmol, Alvares, Danielle, Choi, Stephen, Dwyer, Heather, Flores, Kathrina, McCartney, Colin, Somascanthan, Priya, Carroll, Jo, Pazmino-Canizares, Janneth, Ami, Noam, Chan, Vincent, Perlas, Anahi, Argue, Ruth, Huang, Yang, Lavis, Katie, Mayson, Kelly, Cao, Ying, Gao, Hong, Hu, Tingju, Lv, Jie, Yang, Jian, Yang, Yang, Zhong, Yi, Zhou, Jing, Zou, Xiaohua, He, Miao, Li, Xiaoying, Luo, Dihuan, Wang, Haiying, Yu, Tian, Chen, Liyong, Wang, Lijun, Cai, Yunfei, Cao, Zhongming, Li, Yanling, Lian, Jiaxin, Sun, Haiyun, Wang, Sheng, Wang, Zhipeng, Wang, Kenru, Zhu, Yi, Du, Xindan, Fan, Hao, Fu, Yunbin, Huang, Lixia, Huang, Yanming, Hwan, Haifang, Luo, Hong, Qu, Pi-Sheng, Tao, Fan, Wang, Zhen, Wang, Guoxiang, Wang, Shun, Zhang, Yan, Zhang, Xiaolin, Chen, Chao, Wang, Weixing, Liu, Zhengyuan, Fan, Lihua, Tang, Jing, Chen, Yijun, Chen, Yongjie, Han, Yangyang, Huang, Changshun, Liang, Guojin, Shen, Jing, Wang, Jun, Yang, Qiuhong, Zhen, Jungang, Zhou, Haidong, Chen, Junping, Chen, Zhang, Li, Xiaoyu, Meng, Bo, Ye, Haiwang, Zhang, Xiaoyan, Bi, Yanbing, Cao, Jianqiao, Guo, Fengying, Lin, Hong, Liu, Yang, Lv, Meng, Shi, Pengcai, Song, Xiumei, Sun, Chuanyu, Sun, Yongtao, Wang, Yuelan, Wang, Shenhui, Zhang, Min, Chen, Rong, Hou, Jiabao, Leng, Yan, Meng, Qing-tao, Qian, Li, Shen, Zi-ying, Xia, Zhong-yuan, Xue, Rui, Zhang, Yuan, Zhao, Bo, Zhou, Xian-jin, Chen, Qiang, Guo, Huinan, Guo, Yongqing, Qi, Yuehong, Wang, Zhi, Wei, Jianfeng, Zhang, Weiwei, Zheng, Lina, Bao, Qi, Chen, Yaqiu, Chen, Yijiao, Fei, Yue, Hu, Nianqiang, Hu, Xuming, Lei, Min, Li, Xiaoqin, Lv, Xiaocui, Miao, Fangfang, Ouyang, Lingling, Qian, Lu, Shen, Conyu, Sun, Yu, Wang, Yuting, Wang, Dong, Wu, Chao, Xu, Liyuan, Yuan, 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Cristina, Tosa, Flaviu, Tranca, Sebastian, Copotoiu, Sanda Maria, Ungureanu, Liviu Lucian, Harsan, Cristian Remus, Papurica, Marius, Cernea, Daniela Denisa, Dragoescu, Nicoleta Alice, CarmenVaida, Laura Aflori, Ciobotaru, Oana Roxana, Aignatoaie, Mariana, Carp, Cristina Paula, Cobzaru, Isabelle, Mardare, Oana, Purcarin, Bianca, Tutunaru, Valentin, Ionita, Victor, Arustei, Mirela, Codita, Anisoara, Busuioc, Mihai, Chilinciuc, Ion, Ciobanu, Cristina, Belciu, Ioana, Tincu, Eugen, Blaj, Mihaela, Grosu, Ramona - Mihaela, Sandu, Gigel, Bruma, Dana, Corneci, Dan, Dutu, Madalina, Krepil, Adriana, Copaciu, Elena, Dumitrascu, Clementina Oana, Jemna, Ramona, Mihaescu, Florentina, Petre, Raluca, Tudor, Cristina, Ursache, Elena, Kulikov, Alexander, Lubnin, Andrey, Grigoryev, Evgeny, Pugachev, Stanislav, Tolmasov, Alexander, Hussain, Ayyaz, Ilyina, Yana, Roshchina, Anna, Iurin, Aleksandr, Chazova, Elena, Dunay, Artem, Karelov, Alexey, Khvedelidze, Irina, Voldaeva, Olga, Belskiy, Vladislav, Dzhamullaev, Parvin, Grishkowez, Elena, Kretov, Vladimir, Levin, Valeriy, Molkov, Aleksandr, Puzanov, Sergey, Samoilenko, Aleksandr, Tchekulaev, Aleksandr, Tulupova, Valentina, Utkin, Ivan, Allorto, Nikki Leigh, Bishop, David Gray, Builu, Pierre Monji, Cairns, Carel, Dasrath, Ashish, de Wet, Jacques, Hoedt, Marielle den, Grey, Ben, Hayes, Morgan Philip, Küsel, Belinda Senta, Shangase, Nomcebo, Wise, Robert, Cacala, Sharon, Farina, Zane, Govindasamy, Vishendran, Kruse, Carl-Heinz, Lee, Carolyn, Marais, Leonard, Naidoo, Thinagrin Dhasarthun, Rajah, Chantal, Rodseth, Reitze Nils, Ryan, Lisa, von Rhaden, Richard, Adam, Suwayba, Alphonsus, Christella, Ameer, Yusuf, Anderson, Frank, Basanth, Sujith, Bechan, Sudha, Bhula, Chettan, Biccard, Bruce M., Biyase, Thuli, Buccimazza, Ines, Cardosa, Jorge, Chen, James, Daya, Bhavika, Drummond, Leanne, Elabib, Ali, Abdel Goad, Ehab Helmy, Goga, Ismail E., Goga, Riaz, Harrichandparsad, R., Hodgson, Richard E., Jordaan, J., Kalafatis, Nicky, Kampik, Christian, Landers, A.T., Loots, Emil, Madansein, Rajhmum, Madaree, Anil, Madiba, Thandinkosi E., Manzini, Vukani T., Mbuyisa, Mbali, Moodley, Rajan, Msomi, Mduduzi, Mukama, Innocent, Naidoo, Desigan, Naidoo, Rubeshan, Naidu, Tesuven K., Ntloko, Sindiswa, Padayachee, Eneshia, Padayachee, Lucelle, Phaff, Martijn, Pillay, Bala, Pillay, Desigan, Pillay, Lutchmee, Ramnarain, Anupa, Ramphal, Suren R., Ryan, Paul, Saloojee, Ahmed, Sebitloane, Motshedisi, Sigcu, Noluyolo, Taylor, Jenna L., Torborg, Alexandra, Visser, Linda, Anderson, Philip, Conradie, Alae, de Swardt, Mathew, de Villiers, Martin, Eikman, Johan, Liebenberg, Riaan, Mouton, Johan, Paton, Abbey, van der Merwe, Louwrence, Wilscott-Davids, Candice, Barrett, Wendy Joan, Bester, Marlet, de Beer, Johan, Geldenhuys, Jacques, Gouws, Hanni, Potgieter, Jan-Hendrik, Strydom, Magdel, WilberforceTurton, Edwin, Chetty, Rubendraj R., Chirkut, Subash, Cronje, Larissa, de Vasconcellos, Kim, Dube, Nokukhanya Z., Gama, N. Sibusiso, Green, Garyth M., Green-Thompson, Randolph, Kinoo, Suman Mewa, Kistnasami, Prenolin, Maharaj, Kapil, Moodley, Manogaran S., Mothae, Sibongile J., Naidoo, Ruvashni, Aslam F Noorbhai, M., Rughubar, Vivesh, Reddy, Jenendhiran, Singh, Avesh, Skinner, David L., Smith, Murray J., Singh, Bhagwan, Misra, Ravi, Naidoo, Maheshwar, Ramdharee, Pireshin, Selibea, Yvonne, Sewpersad, Selina, Sham, Shailendra, Wessels, Joseph D., Africander, Cucu, Bejia, Tarek, Blakemore, Stephen P., Botes, Marisa, Bunwarie, Bimalshakth, Hernandez, Carlos B., Jeeraz, Mohammud A., Legutko, Dagmara A., Lopez, Acela G., De Meyer, Jenine N., Muzenda, Tanaka, Naidoo, Noel, Patel, Maryam, Pentela, Rao, Junge, Marina, Mansoor, Naj, Rademan, Lana, Scislowski, Pawel, Seedat, Ismail, van den Berg, Bianca, van der Merwe, Doreen, van Wyk, Steyn, Govender, Komalan, Naicker, Darshan, Ramjee, Rajesh, Saley, Mueen, Kuhn, Warren Paul, Matos-Puig, Roel, Alberto Lisi, Zaheer Moolla, Perez, Gisela, Beltran, Anna Valle, Lozano, Angels, Navarro, Carlos Delgado, Duca, Alejandro, Ernesto, Ernesto Pastor Martinez, Ferrando, Carlos, Fuentes, Isabel, García-Pérez, Maria Luisa, Gracia, Estefania, Palomares, Ana Izquierdo, Katime, Antonio, Miñana, Amanda, Incertis, Raul Raul, Romero, Esther, Romero Garcia, Carolina Soledad, Rubio, Concepcion, Artiles, Tania Socorro, Soro, Marina, Valls, Paola, Laguarda, Gisela Alaman, Benavent, Pau, Cuenca, Vicente Chisbert, Cueva, Andreu, Lafuente, Matilde, Parra, Asuncion Marques, Rodrigo, Alejandra Romero, Sanchez-Morcillo, Silvia, Tormo, Sergi, Redondo, Francisco Javier, De Andrés Ibanez, José Antonio, Diago, Lorena Gómez, José Hernández Cádiz, Maria, Manuel, Granell Gil, Peris, Raquel, Saiz, Cristina, Vivo, Jose Tatay, Soto, Maria Teresa Tebar, Brunete, Tamara, Cancho, David, Delgado García, David R., Zamudio, Diana, Del Valle, Santiago Garcia, Serrano, M. Luz, Alonso, Eduardo, Anillo, Victor, Maseda, Emilio, Salgado, Patricia, Suarez, Luis, Suarez-de-la-Rica, Alejandro, Villagrán, María José, Alonso, José Ignacio, Cabezuelo, Estefania, Garcia-Saiz, Irene, Lopez del Moral, Olga, Martín, Silvia, Gonzalez, Alba Perez, Doncel, Ma Sherezade Tovar, Vera, Martin Agüero, José Ávila Sánchez, Francisco, Castaño, Beatriz, Moreira, Beatriz Castaño, Risco, Sahely Flores, Martín, Daniel Paz, Martín, Fernando Pérez, Poza, Paloma, Ruiz, Adela, Serna Martínez, Wilson Fabio, Vicente, Bárbara Vázquez, Dominguez, Saul Velaz, Fernández, Salvador, Munoz-López, Alfonso, Bernat, Maria Jose, Mas, Arantxa, Planas, Kenneth, Jawad, Monir, Saeed, Yousif, Hedin, Annika, Levander, Helena, Holmström, Sandra, Lönn, David, Zoerner, Frank, Åkring, Irene, Widmark, Carl, Zettergren, Jan, Liljequist, Victor Aspelund, Nystrom, Lena, Odeberg-Wernerman, Suzanne, Oldner, Anders, Fagerlund, Malin Jonsson, Reje, Patrik, Lyckner, Sara, Sperber, Jesper, Adolfsson, Anne, Klarin, Bengt, Ögren, Katrin, Barras, Jean-Pierre, Bührer, Thomas, Despotidis, Vasileios, Helmy, Naeder, Holliger, Stephan, Raptis, Dimitri Aristotle, Schmid, Roger, Meyer, Antoine, Jaquet, Yves, Kessler, Ulf, Muradbegovic, Mirza, Nahum, Solange R., Rotunno, Teresa, Schiltz, Boris, Voruz, François, Worreth, Marc, Christoforidis, Dimitri, Popeskou, Sotirios Georgios, Furrer, Markus, Prevost, Gian Andrea, Stocker, Andrea, Lang, Klaus, Breitenstein, Stefan, Ganter, Michael T., Geisen, Martin, Soll, Christopher, Korkmaz, Michelle, Lubach, Iris, Schmitz, Michael, Meyer zu Schwabedissen, Moritz, Moritz, Meyer zu Schwabedissen, Zingg, Urs, Hillermann, Thomas, Wildi, Stefan, Pinto, Bernardo Bollen, Walder, Bernhard, Mariotti, Giustina, Slankamenac, Ksenija, Namuyuga, Mirioce, Kyomugisha, Edward, Kituuka, Olivia, Shikanda, Anne Wesonga, Kakembo, Nasser, Tom, Charles Otim, Antonina, Webombesa, Bua, Emmanuel, Ssettabi, Eden Michael, Epodoi, Joseph, Kabagenyi, Fiona, Kirya, Fred, Dempsey, Ged, Seasman, Colette, Nawaz Khan, Raja Basit, Kurasz, Claire, Macgregor, Mark, Shawki, Burhan, Francis, Daren, Hariharan, Vimal, Chau, Simon, Ellis, Kate, Butt, Georgina, Chicken, Dennis-Wayne, Christmas, Natasha, Allen, Samantha, Daniel, Gayatri Daniel, Dempster, Angie, Kemp, Juliette, Matthews, Lewis, Mcglone, Philip, Tambellini, Joanne, Trodd, Dawn, Freitas, Katie, Garg, Atul, Gupta, Janesh Kumar, Karpate, Shilpaja, Kulkarni, Aditi, O'Hara, Chloe, Troko, Jtroko, Angus, Kirsty, Bradley, Jacqueline, Brennan, Emma, Brooks, Carolyn, Brown, Janette, Brown, Gemma, Finch, Amanda, Gratrix, Karen, Hesketh, Sue, Hill, Gillian, Jeffs, Carol, Morgan, Maureen, Pemberton, Chris, Slawson, Nicola, Spickett, Helen, Swarbrick, Gemma, Thomas, Megan, Van Duyvenvoorde, Greta, Brennan, Andrew, Briscoe, Richard, Cooper, Sarah, Lawton, Tom, Northey, Martin, Senaratne, Rashmi, Stanworth, Helen, Burrows, Lorna, Cain, Helen, Craven, Rachael, Davies, Keith, Jonas, Attila, Pachucki, Marcin, Walkden, Graham, Davies, Helen, Gudaca, Mariethel, Hobrok, Maria, Arawwawala, Dilshan, Fergey, Lauren, Gardiner, Matthew, Gunn, Jacqueline, Johnson, Lyndsay, Lofting, Amanda, Lyle, Amanda, Neela, Fiona Mc, Smolen, Susan, Topliffe, Joanne, Williams, Sarah, Bland, Martin, Balaji, Packianathaswamy, Kaura, Vikas, Lanka, Prasad, Smith, Neil, Ahmed, Ahmed, Myatt, John, Shenoy, Ravikiran, Soon, Wai Cheong, Tan, Jessica, Karadia, Sunny, Self, James, Durant, Emma, Tripathi, Shiva, Bullock, Clare, Campbell, Debbie, Ghosh, Alison, Hughes, Thomas, Zsisku, Lajos, Bengeri, Sheshagiri, Cowton, Amanda, Khalid, Mohammed Shazad, Limb, James, McAdam, Colin, Porritt, Mandy, Rafi, M. Amir, Shekar, Priya, Adams, David, Harden, Catherine, Hollands, Heidi, King, Angela, March, Linda, Minto, Gary, Patrick, Abigail, Squire, Rosalyn, Waugh, Darren, Kumara, Paramesh, Simeson, Karen, Yarwood, Jamie, Browning, Julie, Hatton, Jonathan, Julian, Howes, Mitra, Atideb, Newton, Maria, Pernu, Pawan Kootelu, Wilson, Alison, Commey, Thelma, Foot, Helen, Glover, Lyn, Gupta, Ajay, Lancaster, Nicola, Levin, Jill, Mackenzie, Felicity, Mestanza, Claire, Nofal, Emma, Pout, Lauren, Varden, Rosanna, Wild, Jonathan, Jones, Stephanie, Moreton, Sarah, Pulletz, Mark, Davies, Charlotte, Martin, Matthew, Thomas, Sian, Burns, Karen, McArthur, Carol, Patel, Panna, Lau, Gary, Rich, Natalie, Davis, Fiona, Lyons, Rachel, Port, Beth, Prout, Rachel, Smith, Christopher, Adelaja, Yemi, Bennett, Victoria, Bidd, Heena, Dumitrescu, Alexandra, Murphy, Jacqui Fox, Keen, Abigail, Mguni, Nhlanhla, Ong, Cheng, Adams, George, Boshier, Piers, Brown, Richard, Butryn, Izabella, Chatterjee, Jayanta, Freethy, Alexander, Lockwood, Geoffrey, Tsakok, Maria, Tsiligiannis, Sophia, Peat, William, Stephenson, Lorraine, Bradburn, Mike, Pick, Sara, Cunha, Pedro, Olagbaiye, Olufemi, Tayeh, Salim, Packianathaswamy, Balaji, Abernethy, Caroline, Balasubramaniam, Madhu, Bennett, Rachael, Bolton, David, Martinson, Victoria, Naylor, Charde, Bell, Stephanie, Heather, Blaylock, Kushakovsky, Vlad, Alcock, Liam, Alexander, Hazel, Anderson, Colette, Baker, Paul, Brookes, Morag, Cawthorn, Louise, Cirstea, Emanuel, Clarkson, Rachel, Colling, Kerry, Coulter, Ian, Das, Suparna, Haigh, Kathryn, Hamdan, Alhafidz, Hugill, Keith, Kottam, Lucksy, Lisseter, Emily, Mawdsley, Matthew, McGivern, Julie, Padala, Krishnaveni, Phelps, Victoria, Ramesh kumar, Vineshykaa, Stewart, Kirsten, Towse, Kayley, Tregonning, Julie, Vahedi, Ali, Walker, Alycon, Baines, Duncan, Bilolikar, Anjali, Chande, Shiv, Copley, Edward, Dunk, Nigel, Kulkarni, Raghavendra, Kumar, Pawan, Metodiev, Yavor, Ncomanzi, Dumisani, Raithatha, Bhavesh, Raymode, Parizade, Szafranski, Jan, Twohey, Linda, Watt, Philip, Weatherall, Lucie, Weatherill, J., Whitman, Zoe, Wighton, Elinor, Abayasinghe, Chamika, Chan, Alexander, Darwish, Sharif, Gill, James, Glasgow, Emma, Hadfield, Daniel, Harris, Clair, Hopkins, Phil, Kochhar, Arun, Kunst, Gudrun, Mellis, Clare, Pool, Andrew, Riozzi, Paul, Selman, Andrew, Smith, Emma-Jane, Vele, Liana, Gercek, Yuksel, Guy, Kramer, Holden, Douglas, Watson, Nicholas, Whysall, Karen, Andreou, Prematie, Hales, Dawn, Thompson, Jonathan, Bowrey, Sarah, McDonald, Shara, Gilmore, Jemma, Hills, Vicky, Kelly, Chan, Kelly, Sinead, Lloyd, Geraint, Abbott, Tom, Gall, Lewis, Torrance, Hew, Vivian, Mark, Berntsen, Emer, Nolan, Tracey, Turner, Angus, Vohra, Akbar, Brown, Andrew, Clark, Richard, Coughlan, Elaine, Daniel, Conway, Patvardhan, Chinmay, Pearson, Rachel, Predeep, Sheba, Saad, Hesham, Shanmugam, Mohanakrishnan, Varley, Simon, Wylie, Katharine, Cooper, Lucy, Makowski, Arystarch, Misztal, Beata, Moldovan, Eliza, Pegg, Claire, Donovan, Andrew, Foot, Jayne, Large, Simon, Claxton, Andrew, Netke, Bhagyashree, Armstrong, Richard, Calderwood, Claire, Kwok, Andy, Mohr, Otto, Oyeniyi, Peter, Patnaik, Lisa, Post, Benjamin, Ali, Sarah, Arshad, Homa, Baker, Gerard, Brenner, Laura, Brincat, Maximilian, Brunswicker, Annemarie, Cox, Hannah, Cozar, Octavian Ionut, Cheong, Edward, Durst, Alexander, Fengas, Lior, Flatt, Jim, Glister, Georgina, Narwani, Vishal, Photi, Evangelos, Rankin, Adeline, Rosbergen, Melissa, Tan, Mark, Beaton, Ceri, Horn, Rachel, Hunt, Jane, Rousseau, Guy, Stancombe, Lucia, Absar, Mohammed, Allsop, Joanne, Drinkwater, Zoe, Hodgkiss, Tracey, Smith, Kirsty, Brown, Jamie, Alexander-Sefre, Farhad, Campey, Lorraine, Dudgeon, Lucy, Hall, Kathryn, Hitchcock, Rachael, James, Lynne, Smith, Kate, Winstone, Ulrika, Ahmad, Norfaizan, Bauchmuller, Kris, Harrison, Jonathan, Jeffery, Holly, Miller, Duncan, Pinder, Angela, Pothuneedi, Sailaja, Rosser, Jonathan, Sanghera, Sumayer, Swift, Diane, Walker, Rachel, Bester, Delia, Cavanagh, Sarah, Cripps, Heather, Daniel, Harvey, Lynch, Julie, Paton, Alison, Pyke, Shirley, Scholefield, John, Whitworth, Helen, Bottrill, Fiona, Ramalingam, Ganesh, Webb, Stephen, Akerman, Nik, Antill, Philip, Bourner, Lynsey, Buckley, Sarah, Castle, Gail, Charles, Rob, Eggleston, Christopher, Foster, Rebecca, Gill, Satwant, Lindley, Kate, Lklouk, Mohamed, Lowery, Tracey, Martin, Oliver, Milne, David, O'Connor, Patrick, Ratcliffe, Andrew, Rose, Alastair, Smith, Annie, Varma, Sandeep, Ward, Jackie, Barcraft-Barnes, Helena, Camsooksai, Julie, Colvin, Carolyn, Reschreiter, Henrik, Tbaily, Lee, Venner, Nicola, Hamilton, Caroline, Kelly, Lewis, Toth-Tarsoly, Piroska, Dodsworth, Kerry, Foord, Denise, Gordon, Paul, Hawes, Elizabeth, Lamb, Nikki, Mouland, Johanna, Nightingale, Jeremy, Rose, Steve, Schrieber, Joe, Al'Amri, Khalid, Aladin, Hafiz, Arshad, Mohammed Asif, Barraclough, James, Bentley, Conor, Bergin, Colin, Carrera, Ronald, Clarkson, Aisling, Collins, Michelle, Cooper, Lauren, Denham, Samuel, Griffiths, Ewen, Ip, Peter, Jeyanthan, Somasundaram, Joory, Kavita, Kaur, Satwant, Marriott, Paul, Mitchell, Natalie, Nagaiah, Sukumar, Nilsson, Annette, Parekh, Nilesh, Pope, Martin, Seager, Joseph, Serag, Hosam, Tameem, Alifia, Thomas, Anna, Thunder, Joanne, Torrance, Andrew, Vohra, Ravinder, Arlo whitehouse, Wong, Tony, Blunt, Mark, Wong, Kate, Giles, Julian, Reed, Isabelle, Weller, Debbie, Bell, Gillian, Birch, Julie, Damant, Rose, Maiden, Jane, Mewies, Clare, Prince, Claire, Radford, Jane, Reynolds, Tim, Balain, Birender, Banerjee, Robin, Barnett, Andrew, Burston, Ben, Davies, Kirsty, Edwards, Jayne, Evans, Chris, Ford, David, Gallacher, Pete, Hill, Simon, Jaffray, David, Karlakki, Sudheer, Kelly, Cormac, Kennedy, Julia, Kiely, Nigel, Lewthwaite, Simon, Marquis, Chris, Ockendon, Matthew, Phillips, Stephen, Pickard, Simon, Richardson, James, Roach, Richard, Smith, Tony, Spencer-Jones, Richard, Steele, Niall, Steen, Julie, Van Liefland, Marck, White, Steve, Faulds, Matthew, Harris, Meredyth, Kelly, Carrie, Nicol, Scott, Pearson, Sally Anne, Chukkambotla, Srikanth, Andrew, Alyson, Attrill, Elizabeth, Campbell, Graham, Datson, Amanda, Fouracres, Anna, Graterol, Juan, Graves, Lynne, Hong, Bosun, Ishimaru, Alexander, Karthikeyan, Arvind, King, Helen, Lawson, Tom, Lee, Gregory, Lyons, Saoirse, Hall, Andrew Macalister, Mathoulin, Sophie, Mcintyre, Eilidh, Mclaughlin, Danny, Mulcahy, Kathleen, Paddle, Jonathan, Ratcliffe, Anna, Robbins, James, Sung, Weilin, Tayo, Adeoluwa, Trembath, Lisa, Venugopal, Suneetha, Walker, Robert, Wigmore, Geoffrey, Boereboom, Catherine, Downes, Charlotte, Humphries, Ryan, Melbourne, Susan, Smith, Coral, Tou, Samson, Ullah, Shafa, Batchelor, Nick, Boxall, Leigh, Broomby, Rupert, Deen, Tariq, Hellewell, Alistair, Helliwell, Laurence, Hutchings, Melanie, Hutchins, David, Keenan, Samantha, Mackie, Donna, Potter, Alison, Smith, Frances, Stone, Lucy, Thorpe, Kevin, Wassall, Richard, Woodgate, Andrew, Baillie, Shelley, Campbell, Tara, James, Sarah, King, Chris, Marques de Araujo, Daniela, Martin, Daniel, Morkane, Clare, Neely, Julia, Rajendram, Rajkumar, Burton, Megan, James, Kathryn, Keevil, Edward, Minik, Orsolya, Morgan, Jenna, Musgrave, Anna, Rajanna, Harish, Roberts, Tracey, Adamson, Michael, Jumbe, Sandra, Kendall, Jennie, Muthuswamy, Mohan Babu, Anderson, Charlotte, Cruikshanks, Andrew, Wrench, Ian, Zeidan, Lisa, Ardern, Diane, Harris, Benjamin, Hellstrom, Johanna, Martin, Jane, Thomas, Richard, Varsani, Nimu, Brown, Caroline Wrey, Docherty, Philip, Gillies, Michael, McGregor, Euan, Usher, Helen, Craig, Jayne, Smith, Andrew, Ahmad, Tahania, Bodger, Phoebe, Creary, Thais, Fowler, Alexander, Hewson, Russ, Ijuo, Eke, Jones, Timothy, Kantsedikas, Ilya, Lahiri, Sumitra, McLean, Aaron Lawson, Niebrzegowska, Edyta, Phull, Mandeep, Wang, Difei, Wickboldt, Nadine, Baldwin, Jacqueline, Doyle, Donna, Mcmullan, Sean, Oladapo, Michelle, Owen, Thomas, Williams, Alexandra, Daniel, Hull, Gregory, Peter, Husain, Tauqeer, Kirk-Bayley, Justin, Mathers, Edward, Montague, Laura, Harper, Mark, White, Stuart, Jack, James, Ridley, Carrie, Avis, Joanne, Cook, Tim, Dali-Kemmery, Lola, Kerslake, Ian, Lambourne, Victoria, Pearson, Annabel, Boyd, Christine, Callaghan, Mark, Lawson, Cathy, McCrossan, Roopa, Nesbitt, Vanessa, O'connor, Laura, Scott, Julia, Sinclair, Rhona, Farid, Nahla, Morgese, Ciro, Bhatia, Kailash, Karmarkar, Swati, Ahmed, Jamil, Branagan, Graham, Hutton, Monica, Swain, Andrew, Brookes, Jamie, Cornell, Jonathan, Dolan, Rachael, Hulme, Jonathan, Jansen van Vuuren, Amanda, Jowitt, Tom, Kalashetty, Gunasheela, Lloyd, Fran, Patel, Kiran, Sherwood, Nicholas, Brown, Lynne, Chandler, Ben, Deighton, Kerry, Emma, Temlett, Haunch, Kirsty, Cheeseman, Michelle, Dent, Kathy, Garg, Sanjeev, Gray, Carol, Hood, Marion, Jones, Dawn, Juj, Joanne, Rao, Roshan, Walker, Tara, Al Anizi, Mashel, Cheah, Clarissa, Cheing, Yushio, Coutinho, Francisco, Gondo, Prisca, Hadebe, Bernard, Hove, Mazvangu Onie, Ahamed khader, Krishnachetty, Bobby, Rhodes, Karen, Sokhi, Jagdish, Baker, Katie-Anne, Bertram, Wendy, Looseley, Alex, Mouton, Ronelle, Hanna, George, Arnold, Glenn, Arya, Shobhit, Balfoussia, Danai, Baxter, Linden, Harris, James, Jones, Craig, Knaggs, Alison, Markar, Sheraz, Perera, Anisha, Scott, Alasdair, Shida, Asako, Sirha, Ravneet, Wright, Sally, Frost, Victoria, Gray, Catherine, Andrews, Emma, Arrandale, Lindsay, Barrett, Stephen, Cifra, Elna, Cooper, Mariese, Dragnea, Dragos, Elna, Cifra, Maclean, Jennifer, Meier, Sonja, Milliken, Donald, Munns, Christopher, Ratanshi, Nadir, Ramessur, Suneil, Salvana, Abegail, Watson, Anthony, Ali, Hani, Campbell, Gill, Critchley, Rebecca, Endersby, Simon, Hicks, Catherine, Liddle, Alison, Pass, Marc, Ritchie, Charlotte, Thomas, Charlotte, Too, Lingxi, Welsh, Sarah, Gill, Talvinder, Johnson, Joanne, Reed, Joanne, Davis, Edward, Papadopoullos, Sam, Attwood, Clare, Biffen, Andrew, Boulton, Kerenza, Gray, Sophie, Hay, David, Mills, Sarah, Montgomery, Jane, Riddell, Rory, Simpson, James, Bhardwaj, Neeraj, Paul, Elaine, Uwubamwen, Nosakhare, Alexander, Maini, Arrich, James, Arumugam, Swarna, Blackwood, Douglas, Boggiano, Victoria, Brown, Robyn, Chan, Yik Lam, Chatterjee, Devnandan, Chhabra, Ashok, Christian, Rachel, Costelloe, Hannah, Matthewman, Madeline Coxwell, Dalton, Emma, Darko, Julia, Davari, Maria, Dave, Tejal, Deacon, Matthew, Deepak, Shantal, Edmond, Holly, Ellis, Jessica, El-Sayed, Ahmed, Eneje, Philip, English, Rose, Ewe, Renee, Foers, William, Franklin, John, Gallego, Laura, Garrett, Emily, Goldberg, Olivia, Goss, Harry, Greaves, Rosanna, Harris, Rudy, Hennings, Charles, Jones, Eleanor, Kamali, Nelson, Kokkinos, Naomi, Lewis, Carys, Lignos, Leda, Malgapo, Evaleen Victoria, Malik, Rizwana, Milne, Andrew, Mulligan, John-Patrick, Nicklin, Philippa, Palipane, Natasha, Parsons, Thomas, Piper, Rebecca, Prakash, Rohan, Ramesh, Byron, Rasip, Sarah, Reading, Jacob, Rela, Mariam, Reyes, Anna, Stephens, Robert, Rooms, Martin, Shah, Karishma, Simons, Henry, Solanki, Shalil, Spowart, Emma, Stevens, Amy, Thomas, Christopher, Waggett, Helena, Yassaee, Arrash, Kennedy, Anthony, Scott, Sara, Somanath, Sameer, Berg, Andrew, Hernandez, Miguel, Nanda, Rajesh, Tank, Ghanshyambhai, Wilson, Natalie, Wilson, Debbie, Al-Soudaine, Yassr, Baldwin, Matthew, Cornish, Julie, Davies, Zoe, Davies, Leigh, Edwards, Marc, Frewer, Natasha, Gallard, Sian, Glasbey, James, Harries, Rhiannon, Hopkins, Luke, Kim, Taeyang, Koompirochana, Vilavan, Lawson, Simon, Lewis, Megan, Makzal, Zaid, Scourfield, Sarah, Ahmad, Yousra, Bates, Sarah, Blackwell, Clare, Bryant, Helen, Collins, Hannah, Coulter, Suzanne, Cruickshank, Ross, Daniel, Sonya, Daubeny, Thomas, Edwards, Mark, Golder, Kim, Hawkins, Lesley, Helen, Bryant, Hinxman, Honor, Levett, Denny, Salmon, Karen, Seaward, Leanne, Skinner, Ben, Tyrell, Bryony, Wadams, Beverley, Walsgrove, Joseph, Dickson, Jane, Constantin, Kathryn, Karen, Markwell, O'Brien, Peter, O'Donohoe, Lynn, Payne, Hannah, Sundayi, Saul, Walker, Elaine, Brooke, Jenny, Cardy, Jon, Humphreys, Sally, Kessack, Laura, Kubitzek, Christiane, Kumar, Suhas, Cotterill, Donna, Hodzovic, Emil, Hosdurga, Gurunath, Miles, Edward, Saunders, Glenn, Campbell, Marta, Chan, Peter, Jemmett, Kim, Raj, Ashok, Naik, Aditi, Oshowo, Ayo, Ramamoorthy, Rajarajan, Shah, Nimesh, Sylvan, Axel, Blyth, Katharine, Burtenshaw, Andrew, Freeman, David, Johnson, Emily, Lo, Philip, Martin, Terry, Plunkett, Emma, Wollaston, Julie, Allison, Joanna, Carroll, Christine, Craw, Nicholas, Craw, Sarah, Pitt-Kerby, Tressy, Rowland-Axe, Rebecca, Spurdle, Katie, McDonald, Andrew, Simon, Davies, Sinha, Vivek, Smith, Thomas, Banner-Goodspeed, Valerie, Boone, Myles, Campbell, Kathleen, Lu, Fengxin, Scannell, Joseph, Sobol, Julia, Balajonda, Naraida, Clemmons, Karen, Conde, Carlos, Elgasim, Magdi, Funk, Bonita, Hall, Roger, Hopkins, Thomas, Olaleye, Omowunmi, Omer, Omer, Pender, Michelle, Porto, Angelo, Stevens, Alice, Waweru, Peter, Yeh, Erlinda, Bodansky, Daniella, Evans, Adam, Kleopoulos, Steven, Maril, Robert, Mathney, Edward, Sanchez, Angela, Tinuoye, Elizabeth, Bateman, Brian, Eng, Kristen, Jiang, Ning, Ladha, Karim, Needleman, Joseph, Chen, Lee-lynn, Lane, Rondall, Robinowitz, David, Ghushe, Neil, Irshad, Mariam, O'Connor, John, Patel, Samir, Takemoto, Steven, Wallace, Art, Mazzeffi, Michael, Rock, Peter, Wallace, Karin, Zhu, Xiaomao, Chua, Pandora, Mattera, Matthew, Sharar, Rebecca, Thilen, Stephan, Treggiari, Miriam, Morgan, Angela, Sofjan, Iwan, Subramaniam, Kathirvel, Avidan, Michael, Maybrier, Hannah, Muench, Maxwell, Wildes, Troy, Abbott, T.E.F., Ahmad, T., Phull, M.K., Fowler, A.J., Hewson, R., Biccard, B.M., Chew, M.S., Gillies, M., and Pearse, R.M.
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- 2018
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31. A generalized proximity effect model in superconducting bi- and tri-layer films
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Brammertz, G., Poelaert, A., Golubov, A. A., Verhoeve, P., Peacock, A., and Rogalla, H.
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Condensed Matter - Superconductivity - Abstract
This paper presents a general model for calculating the density of states and the Cooper pair potential in proximity coupled superconducting bi- and tri-layer films. The typical example of superconducting tunnel junctions is used to show that the model is working., Comment: 25 pages, 6 figures
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- 2004
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32. Lattice-dynamics of a Disordered solid-solid Interface
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Fagas, G., Kozorezov, A. G., Lambert, C. J., Wigmore, J. K., Peacock, A., Poelaert, A., and Hartog, R. den
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Condensed Matter - Abstract
Generic properties of elastic phonon transport at a disordered interface are studied. The results show that phonon transmittance is a strong function of frequency and the disorder correlation length. At frequencies lower than the van Hove singularity the transmittance at a given frequency increases as the correlation length decreases. At low frequencies, this is reflected by different power-laws for phonon conductance across correlated and uncorrelated disordered interfaces which are in approximate agreement with perturbation theory of an elastic continuum. These results can be understood in terms of simple mosaic and two-colour models of the interface., Comment: 17 pages, 5 figures, submitted to PRB
- Published
- 1999
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33. Comfort in palliative sedation (Compas): a transdisciplinary mixed method study protocol for linking objective assessments to subjective experiences
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Stefaan Six, Steven Laureys, Jan Poelaert, Johan Bilsen, Peter Theuns, and Reginald Deschepper
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Special situations and conditions ,RC952-1245 - Abstract
Abstract Background In case of untreatable suffering at the end of life, palliative sedation may be chosen to assure comfort by reducing the patient’s level of consciousness. An important question here is whether such sedated patients are completely free of pain. Because these patients cannot communicate anymore, caregivers have to rely on observation to assess the patient’s comfort. Recently however, more sophisticated techniques from the neurosciences have shown that sometimes consciousness and pain are undetectable with these traditional behavioral methods. The aim of this study is to better understand how unconscious palliative sedated patients experience the last days of their life and to find out if they are really free of pain. Methods In this study we will observe 40 patients starting with initiation of palliative sedation until death. Assessment of comfort based on behavioral observations will be related with the results from a NeuroSense monitor, an EEG-based monitor used for evaluation of the adequacy of anesthesia and sedation in the operating room and an ECG-based Analgesia Nociception Index (ANI) monitor, which informs about comfort or discomfort condition, based on the parasympathetic tone. An innovative and challenging aspect of this study is its qualitative approach; “objective” and “subjective” data will be linked to achieve a holistic understanding of the study topic. The following data will be collected: assessment of pain/comfort by the patients themselves (if possible) by scoring a Visual Analogue Scale (VAS); brain function monitoring; monitoring of parasympathetic tone; caregivers’ assessment (pain, awareness, communication); relatives’ perception of the quality of the dying process; assessment by 2 trained investigators using observational scales; video and audio registration. Discussion Measuring pain and awareness in non-communicative dying patients is both technically and ethically challenging. ANI and EEG have shown to be promising technologies to detect pain that otherwise cannot be detected with the “traditional” methods. Although these technologies have the potential to provide objective quantifiable indicators for distress and awareness in non-communicative patients, strikingly they have not yet been used to check whether the current assessments for non-communicative patients are reliable. Trial registration The study is registered on ClinicalTrials.gov (Identifier: NCT03273244; registration date: 7.9.2017).
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- 2018
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34. Reducing agent can be omitted in the incubation medium of the batch in vitro fermentation model of the pig intestines
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C. Poelaert, G. Nollevaux, C. Boudry, B. Taminiau, C. Nezer, G. Daube, Y.-J. Schneider, D. Portetelle, A. Théwis, and J. Bindelle
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gut fermentation ,in vitro method ,pig ,reducing agent ,pyrosequencing ,Animal culture ,SF1-1100 - Abstract
Over the past decade, in vitro methods have been developed to study intestinal fermentation in pigs and its influence on the digestive physiology and health. In these methods, ingredients are fermented by a bacterial inoculum diluted in a mineral buffer solution. Generally, a reducing agent such as Na2S or cysteine-HCl generates the required anaerobic environment by releasing metabolites similar to those produced when protein is fermented, possibly inducing a dysbiosis. An experiment was conducted to study the impact of two reducing agents on results yielded by such in vitro fermentation models. Protein (soybean proteins, casein) and carbohydrate (potato starch, cellulose) ingredients were fermented in vitro by bacteria isolated from fresh feces obtained from three sows in three carbonate-based incubation media differing in reducing agent: (i) Na2S, (ii) cysteine-HCl and (iii) control with a mere saturation with CO2 and devoid of reducing agent. The gas production during fermentation was recorded over 72 h. Short-chain fatty acids (SCFA) production after 24 and 72 h and microbial composition of the fermentation broth after 24 h were compared between ingredients and between reducing agents. The fermentation residues after 24 h were also evaluated in terms of cytotoxicity using Caco-2 cell monolayers. Results showed that the effect of the ingredient induced higher differences than the reducing agent. Among the latter, cysteine-HCl induced the strongest differences compared with the control, whereas Na2S was similar to the control for most parameters. For all ingredients, final gas produced per g of substrate was similar (P>0.10) for the three reducing agents whereas the maximum rate of gas production (Rmax) was reduced (P0.10) after 24 h of fermentation with Na2S and in the control without reducing agent. Molar ratios of branched chain-fatty acids were higher (P
- Published
- 2018
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35. Educating anaesthetists on neuromuscular monitoring: the challenges continue
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Poelaert, Jan, primary and Hunter, Jennifer M., additional
- Published
- 2023
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36. The pathophysiological impact of intra-abdominal hypertension in pigs
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Wise, Robert, primary, Rodseth, Reitze, additional, Párraga-Ros, Ester, additional, Latorre, Rafael, additional, López Albors, Octavio, additional, Correa-Martín, Laura, additional, M. Sánchez-Margallo, Francisco, additional, Eugenia Candanosa-Aranda, Irma, additional, Poelaert, Jan, additional, Castellanos, Gregorio, additional, and L. N. G. Malbrain, Manu, additional
- Published
- 2023
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37. Multi-organ point-of-care ultrasound for COVID-19 (PoCUS4COVID): international expert consensus
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Hussain, Arif, Via, Gabriele, Melniker, Lawrence, Goffi, Alberto, Tavazzi, Guido, Neri, Luca, Villen, Tomas, Hoppmann, Richard, Mojoli, Francesco, Noble, Vicki, Zieleskiewicz, Laurent, Blanco, Pablo, Ma, Irene W. Y., Wahab, Mahathar Abd., Alsaawi, Abdulmohsen, Al Salamah, Majid, Balik, Martin, Barca, Diego, Bendjelid, Karim, Bouhemad, Belaid, Bravo-Figueroa, Pablo, Breitkreutz, Raoul, Calderon, Juan, Connolly, Jim, Copetti, Roberto, Corradi, Francesco, Dean, Anthony J., Denault, André, Govil, Deepak, Graci, Carmela, Ha, Young-Rock, Hurtado, Laura, Kameda, Toru, Lanspa, Michael, Laursen, Christian B., Lee, Francis, Liu, Rachel, Meineri, Massimiliano, Montorfano, Miguel, Nazerian, Peiman, Nelson, Bret P., Neskovic, Aleksandar N., Nogue, Ramon, Osman, Adi, Pazeli, José, Pereira-Junior, Elmo, Petrovic, Tomislav, Pivetta, Emanuele, Poelaert, Jan, Price, Susanna, Prosen, Gregor, Rodriguez, Shalim, Rola, Philippe, Royse, Colin, Chen, Yale Tung, Wells, Mike, Wong, Adrian, Xiaoting, Wang, Zhen, Wang, and Arabi, Yaseen
- Published
- 2020
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38. Acceptance of mHealth among health professionals: a case study on anesthesia practitioners
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Carvalho, Hugo, Verdonck, Michael, Forget, Patrice, and Poelaert, Jan
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- 2020
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39. Electrocardiographic Effects of Propofol versus Etomidate in Patients with Brugada Syndrome
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Flamée, Panagiotis, Varnavas, Varnavas, Dewals, Wendy, Carvalho, Hugo, Cools, Wilfried, Bhutia, Jigme Tshering, Beckers, Stefan, Umbrain, Vincent, Verborgh, Christian, Forget, Patrice, Chierchia, Gian-Battista, Brugada, Pedro, Poelaert, Jan, and de Asmundis, Carlo
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- 2020
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40. Enterovirus D-68 Infection of Primary Rat Cortical Neurons: Entry, Replication, and Functional Consequences
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Poelaert, Katrien C K, van Kleef, Regina G D M, Liu, Mengying, van Vliet, Arno, Lyoo, Heyrhyoung, Gerber, Lora-Sophie, Narimatsu, Yoshiki, Büll, Christian, Clausen, Henrik, de Vries, Erik, Westerink, Remco H S, van Kuppeveld, Frank J M, Poelaert, Katrien C K, van Kleef, Regina G D M, Liu, Mengying, van Vliet, Arno, Lyoo, Heyrhyoung, Gerber, Lora-Sophie, Narimatsu, Yoshiki, Büll, Christian, Clausen, Henrik, de Vries, Erik, Westerink, Remco H S, and van Kuppeveld, Frank J M
- Abstract
Enterovirus D68 (EV-D68) is an emerging pathogen associated with mild to severe respiratory disease. Since 2014, EV-D68 is also linked to acute flaccid myelitis (AFM), causing paralysis and muscle weakness in children. However, it remains unclear whether this is due to an increased pathogenicity of contemporary EV-D68 clades or increased awareness and detection of this virus. Here, we describe an infection model of primary rat cortical neurons to study the entry, replication, and functional consequences of different EV-D68 strains, including historical and contemporary strains. We demonstrate that sialic acids are important (co)receptors for infection of both neurons and respiratory epithelial cells. Using a collection of glycoengineered isogenic HEK293 cell lines, we show that sialic acids on either N-glycans or glycosphingolipids can be used for infection. Additionally, we show that both excitatory glutamatergic and inhibitory GABA-ergic neurons are susceptible and permissive to historical and contemporary EV-D68 strains. EV-D68 infection of neurons leads to the reorganization of the Golgi-endomembranes forming replication organelles, first in the soma and later in the processes. Finally, we demonstrate that the spontaneous neuronal activity of EV-D68-infected neuronal network cultured on microelectrode arrays (MEA) is decreased, independent of the virus strain. Collectively, our findings provide novel insights into neurotropism and -pathology of different EV-D68 strains, and argue that it is unlikely that increased neurotropism is a recently acquired phenotype of a specific genetic lineage. IMPORTANCE Acute flaccid myelitis (AFM) is a serious neurological illness characterized by muscle weakness and paralysis in children. Since 2014, outbreaks of AFM have emerged worldwide, and they appear to be caused by nonpolio enteroviruses, particularly enterovirus-D68 (EV-D68), an unusual enterovirus that is known to mainly cause respiratory disease. It is unknown whethe
- Published
- 2023
41. Enterovirus D-68 Infection of Primary Rat Cortical Neurons: Entry, Replication, and Functional Consequences
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Virologie, IRAS OH Toxicology, IRAS – One Health Toxicology, Infectious Diseases and Immunology - Virology, Poelaert, Katrien C K, van Kleef, Regina G D M, Liu, Mengying, van Vliet, Arno, Lyoo, Heyrhyoung, Gerber, Lora-Sophie, Narimatsu, Yoshiki, Büll, Christian, Clausen, Henrik, de Vries, Erik, Westerink, Remco H S, van Kuppeveld, Frank J M, Virologie, IRAS OH Toxicology, IRAS – One Health Toxicology, Infectious Diseases and Immunology - Virology, Poelaert, Katrien C K, van Kleef, Regina G D M, Liu, Mengying, van Vliet, Arno, Lyoo, Heyrhyoung, Gerber, Lora-Sophie, Narimatsu, Yoshiki, Büll, Christian, Clausen, Henrik, de Vries, Erik, Westerink, Remco H S, and van Kuppeveld, Frank J M
- Published
- 2023
42. Enterovirus D-68 Infection of Primary Rat Cortical Neurons:Entry, Replication, and Functional Consequences
- Author
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Poelaert, Katrien C.K., van Kleef, Regina G.D.M., Liu, Mengying, van Vliet, Arno, Lyoo, Heyrhyoung, Gerber, Lora Sophie, Narimatsu, Yoshiki, Büll, Christian, Clausen, Henrik, de Vries, Erik, Westerink, Remco H.S., van Kuppeveld, Frank J.M., Poelaert, Katrien C.K., van Kleef, Regina G.D.M., Liu, Mengying, van Vliet, Arno, Lyoo, Heyrhyoung, Gerber, Lora Sophie, Narimatsu, Yoshiki, Büll, Christian, Clausen, Henrik, de Vries, Erik, Westerink, Remco H.S., and van Kuppeveld, Frank J.M.
- Abstract
Enterovirus D68 (EV-D68) is an emerging pathogen associated with mild to severe respiratory disease. Since 2014, EV-D68 is also linked to acute flaccid myelitis (AFM), causing paralysis and muscle weakness in children. However, it remains unclear whether this is due to an increased pathogenicity of contemporary EV-D68 clades or increased awareness and detection of this virus. Here, we describe an infection model of primary rat cortical neurons to study the entry, replication, and functional consequences of different EV-D68 strains, including historical and contemporary strains. We demonstrate that sialic acids are important (co)receptors for infection of both neurons and respiratory epithelial cells. Using a collection of glycoengineered isogenic HEK293 cell lines, we show that sialic acids on either N-glycans or glycosphingolipids can be used for infection. Additionally, we show that both excitatory glutamatergic and inhibitory GABA-ergic neurons are susceptible and permissive to historical and contemporary EV-D68 strains. EV-D68 infection of neurons leads to the reorganization of the Golgi-endomembranes forming replication organelles, first in the soma and later in the processes. Finally, we demonstrate that the spontaneous neuronal activity of EV-D68-infected neuronal network cultured on microelectrode arrays (MEA) is decreased, independent of the virus strain. Collectively, our findings provide novel insights into neurotropism and -pathology of different EV-D68 strains, and argue that it is unlikely that increased neurotropism is a recently acquired phenotype of a specific genetic lineage. IMPORTANCE Acute flaccid myelitis (AFM) is a serious neurological illness characterized by muscle weakness and paralysis in children. Since 2014, outbreaks of AFM have emerged worldwide, and they appear to be caused by nonpolio enteroviruses, particularly enterovirus-D68 (EV-D68), an unusual enterovirus that is known to mainly cause respiratory disease. It is unknown whethe
- Published
- 2023
43. Beyond Gut Instinct: Metabolic Short-Chain Fatty Acids Moderate the Pathogenesis of Alphaherpesviruses
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Katrien C. K. Poelaert, Jolien Van Cleemput, Kathlyn Laval, Sarah Descamps, Herman W. Favoreel, and Hans J. Nauwynck
- Subjects
alphaherpesviruses ,varicellovirus ,short-chain fatty acids ,pathogenesis ,respiratory tract ,viremia ,Microbiology ,QR1-502 - Abstract
Short-chain fatty acids (SCFA), such as sodium butyrate (SB), sodium propionate (SPr), and sodium acetate (SAc), are metabolic end-products of the fermentation of dietary fibers. They are linked with multiple beneficial effects on the general mammalian health, based on the sophisticated interplay with the host immune response. Equine herpesvirus 1 (EHV1) is a major pathogen, which primarily replicates in the respiratory epithelium, and disseminates through the body via a cell-associated viremia in leukocytes, even in the presence of neutralizing antibodies. Infected monocytic CD172a+ cells and T-lymphocytes transmit EHV1 to the endothelium of the endometrium or central nervous system (CNS), causing reproductive or neurological disorders. Here, we questioned whether SCFA have a potential role in shaping the pathogenesis of EHV1 during the primary replication in the URT, during the cell-associated viremia, or at the level of the endothelium of the pregnant uterus and/or CNS. First, we demonstrated the expression of SCFA receptors, FFA2 and FFA3, within the epithelium of the equine respiratory tract, at the cell surface of immune cells, and equine endothelium. Subsequently, EHV1 replication was evaluated in the URT, in the presence or absence of SB, SPr, or SAc. In general, we demonstrated that SCFA do not affect the number of viral plaques or virus titer upon primary viral replication. Only SB and SPr were able to reduce the plaque latitudes. Similarly, pretreatment of monocytic CD172a+ cells and T-lymphocytes with different concentrations of SCFA did not alter the number of infected cells. When endothelial cells were treated with SB, SPr, or SAc, prior to the co-cultivation with EHV1-inoculated mononuclear cells, we observed a reduced number of adherent immune cells to the target endothelium. This was associated with a downregulation of endothelial adhesion molecules ICAM-1 and VCAM-1 in the presence of SCFA, which ultimately lead to a significant reduction of the EHV1 endothelial plaques. These results indicate that physiological concentrations of SCFA may affect the pathogenesis of EHV1, mainly at the target endothelium, in favor of the fitness of the horse. Our findings may have significant implications to develop innovative therapies, to prevent the devastating clinical outcome of EHV1 infections.
- Published
- 2019
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44. Controlled-release of opioids for improved pain management
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Martin, Charlotte, De Baerdemaeker, Andy, Poelaert, Jan, Madder, Annemieke, Hoogenboom, Richard, and Ballet, Steven
- Published
- 2016
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45. Impact of fatigue on anaesthesia providers
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Annelies Scholliers, Stef Cornelis, Maurizio Tosi, Tine Opsomer, David Shaproski, Caroline Vanlersberghe, Domien Vanhonacker, Jan Poelaert, Lisa Goudman, Maarten Moens, Gerontology, Faculty of Medicine and Pharmacy, Anesthesiology, Supporting clinical sciences, Intensive Care, Anesthesiology research group, Brussels Heritage Lab, Physiotherapy, Human Physiology and Anatomy, Neurosurgery, Pain in Motion, Neuroprotection & Neuromodulation, and Radiology
- Subjects
fatigue risk management systems ,Anesthesiology and Pain Medicine ,non-technical skills ,fatigue ,Medication error ,scoping review ,sleepiness ,anaesthesia providers - Abstract
BACKGROUND: Recently, fatigue has received more attention as a workplace hazard. This scoping review focuses on fatigue in anaesthesia providers. We explore the prevalence of fatigue in anaesthesia providers, and we examine how fatigue impacts their performance. METHODS: A literature search was independently conducted from December 2019 through March 2020. The following four databases were consulted: MEDLINE, CINAHL, EMBASE, and PubPsych. Only studies discussing fatigue in anaesthesia providers were eligible. RESULTS: The initial database search identified a total of 118 studies, of which 30 studies were included in the review. Eight articles concerned the prevalence of fatigue in anaesthesia providers, whereas 22 explored the impact of fatigue on the performance of anaesthesia providers. Up to 60.8% of anaesthesia providers suffered from severe excessive daytime sleepiness, and fatigue was denoted as a common workplace problem in up to 73.1% of anaesthesia providers. Fatigue had a negative influence on medication errors and vigilance, and it decreased the performance of anaesthesia providers during laboratory psychomotor testing. There was a decrease in non-technical skills (notably communication and teamwork) and worsening mood when fatigued. CONCLUSIONS: Based on this scoping review, fatigue is a prevalent a phenomenon that anaesthesia providers cannot ignore. A combination of deterioration in non technical skills, increased medication errors, loss of sustained attention, and psychomotor decline can lead to poorer performance and cause patient harm. Concrete strategies to mitigate fatigue should be developed.
- Published
- 2023
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46. Pollens destroy respiratory epithelial cell anchors and drive alphaherpesvirus infection
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Van Cleemput, Jolien, Poelaert, Katrien C. K., Laval, Kathlyn, Impens, Francis, Van den Broeck, Wim, Gevaert, Kris, and Nauwynck, Hans. J.
- Published
- 2019
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47. Unravelling the first key steps in equine herpesvirus type 5 (EHV5) pathogenesis using ex vivo and in vitro equine models
- Author
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Van Cleemput, Jolien, Poelaert, Katrien C. K., Laval, Kathlyn, and Nauwynck, Hans J.
- Published
- 2019
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48. Enterovirus D-68 Infection of Primary Rat Cortical Neurons: Entry, Replication, and Functional Consequences
- Author
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Poelaert, Katrien C. K., primary, van Kleef, Regina G. D. M., additional, Liu, Mengying, additional, van Vliet, Arno, additional, Lyoo, Heyrhyoung, additional, Gerber, Lora-Sophie, additional, Narimatsu, Yoshiki, additional, Büll, Christian, additional, Clausen, Henrik, additional, de Vries, Erik, additional, Westerink, Remco H. S., additional, and van Kuppeveld, Frank J. M., additional
- Published
- 2023
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- View/download PDF
49. A new minimally invasive technique for lead revision of perc-paddle leads
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De Coster, Olivier, Forget, Patrice, Moens, Maarten, Matic, Milica, Choustoulakis, Lucie, and Poelaert, Jan
- Published
- 2018
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50. Abortigenic but Not Neurotropic Equine Herpes Virus 1 Modulates the Interferon Antiviral Defense
- Author
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Katrien C. K. Poelaert, Jolien Van Cleemput, Kathlyn Laval, Herman W. Favoreel, Gisela Soboll Hussey, Roger K. Maes, and Hans J. Nauwynck
- Subjects
equine herpesvirus 1 ,upper respiratory tract ,innate immunity ,type I interferon ,phenotype ,Microbiology ,QR1-502 - Abstract
Equine herpesvirus 1 (EHV1) is considered as a major pathogen of Equidae, causing symptoms from mild respiratory disease to late-term abortion and neurological disorders. Different EHV1 strains circulating in the field have been characterized to be of abortigenic or neurovirulent phenotype. Both variants replicate in a plaque-wise manner in the epithelium of the upper respiratory tract (URT), where the abortigenic strains induce more prominent viral plaques, compared to the neurovirulent strains. Considering the differences in replication at the URT, we hypothesized that abortigenic strains may show an increased ability to modulate the type I IFN secretion/signaling pathway, compared to strains that display the neurovirulent phenotype. Here, we analyze IFN levels induced by abortigenic and neurovirulent EHV1 using primary respiratory epithelial cells (EREC) and respiratory mucosa ex vivo explants. Similar levels of IFNα (~70 U/ml) were detected in explants inoculated with both types of EHV1 strains from 48 to 72 hpi. Second, EREC and mucosa explants were treated with recombinant equine IFNα (rEqIFNα) or Ruxolitinib (Rux), an IFN signaling inhibitor, prior to and during inoculation with abortigenic or neurovirulent EHV1. Replication of both EHV1 variants was suppressed by rEqIFNα. Further, addition of Rux increased replication in a concentration-dependent manner, indicating an IFN-susceptibility for both variants. However, in two out of three horses, at a physiological concentration of 100 U/ml of rEqIFNα, an increase in abortigenic EHV1 replication was observed compared to 10 U/ml of rEqIFNα, which was not observed for the neurovirulent strains. Moreover, in the presence of Rux, the plaque size of the abortigenic variants remained unaltered, whereas the typically smaller viral plaques induced by the neurovirulent variants became larger. Overall, our results demonstrate the importance of IFNα in the control of EHV1 replication in the URT for both abortigenic and neurovirulent variants. In addition, our findings support the speculation that abortigenic variants of EHV1 may have developed anti-IFN mechanisms that appear to be absent or less pronounced in neurovirulent EHV1 strains.
- Published
- 2018
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