Search

目的分析食管鳞癌（ESCC）中具核梭杆菌（Fn）对内质网应激相关蛋白葡萄糖调节蛋白78（GRP78）及X盒结合蛋白1（XBP1）的诱导效应，并探讨其潜在机制及临床意义。方法采用Fn感染ESCC细胞KYSE150及KYSE140不同时间（12 h、24 h及48 h），通过氧化应激指标试剂盒及Western blot检测各组细胞中氧化应激指标（ROS、MDA与SOD）及GRP78与XBP1的表达情况。实验分为Fn组，Fn+siNC1组，Fn+siGRP78组，Fn+siNC2组及Fn+siXBP1组，比较各组细胞中氧化应激指标、紫杉醇（PTX）应答效力、增殖、侵袭及转移能力差异。采用RNA scope及免疫组化法检测234例ESCC组织及癌旁组织中Fn感染及GRP78与XBP1的表达情况。利用卡方检验分析各因素与患者临床病理特征之间的相关性。采用Kaplan-Meier生存分析比较各因素对患者生存期的影响。结果与Fn未感染的KYSE150及KYSE140组细胞相比，Fn感染组（12 h、24 h及48 h）细胞中ROS与MDA含量逐渐增加，SOD活性逐渐降低，且GRP78及XBP1表达量逐渐增高（均P < 0.05）。与Fn组、Fn+siNC1组及Fn+siNC2组相比，Fn+siGRP78组及Fn+siXBP1组细胞中ROS与MDA含量减少，SOD活性增强，PTX应答效力增强，增殖、侵袭及转移能力减弱（均P < 0.05）。ESCC组织中Fn感染率、GRP78及XBP1阳性率分别为43.16%、69.66%及60.68%，三者具有显著一致性（P < 0.05）。Fn感染阳性、GRP78及XBP1高表达的患者多为有吸烟、饮酒史的男性患者，且肿瘤分化程度低、浸润程度深、淋巴结转移率高、临床分期多为Ⅲ/Ⅳ期。三者阳性组的患者5年生存期均缩短（P < 0.05）。结论Fn可通过诱导GRP78及XBP1高表达，引发内质网应激，促进ESCC恶性演进。

Copyright of Population & Economics / Renkou yu Jingji is the property of Capital University of Economics & Business and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Rural Water & Hydropower is the property of China Rural Water & Hydropower Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Pearl River is the property of Pearl River Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Diamond & Abrasives Engineering is the property of Zhengzhou Research Institute for Abrasives & Grinding and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Coal Geology & Exploration is the property of Xian Research Institute of China Coal Research Institute and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The surface tension and surface expansion viscosity of sodium dodecyl sulfate（SDS）surfactant solutions of different valences and concentrations of inorganic salt systems were measured by the true spherical bubble method. The foaming power and foam stability of the same solution were measured by the Ross-Miles method. The following results are obtained：（I）The surface tension of the SDS solution decreases with the increase of inorganic salt concentrations until a constant value at certain concentration which is set as as a relative concentration of 100%. It is found that the critical micelle concentration（CMC）value has nothing to do with the type of added salt，but only decreases with the increase of the relative concentration of inorganic salt，i. e. ，the CMC value increases with the inorganic salt. The increase in the relative concentration of，from 8. 3 mmol/L without inorganic salt，converges to the minimum value of 3. 0 mmol/L；（II）The surface tension and the corresponding foaming force are in a negative linear relationship，and the surface tension is inferred. The foaming power can be evaluated as that，the surface expansion viscosity and the corresponding foam stability value have a positive linear relationship，from which it is inferred that the surface expansion viscosity can evaluate the foam stability；（III）The foaming power and foam stability of SDS surfactants increases with the valence of cations（Na+，Cu2+，Fe3+ ). [ABSTRACT FROM AUTHOR]

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of CT Theory & Applications is the property of Editorial Department of CT Theory & Applications and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of South Architecture / Nanfang Jianzhu is the property of South Architecture Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

2016年7月中下旬,对贵州桐梓黄莲柏菁自然保护区鸟类资源进行了实地考察,记录夏季鸟类112种,隶属12目36科,优势种为红嘴相思鸟(Leiothrix lutea)、强脚树莺(Cettia fortipes)、绿翅短脚鹎(Hypsipetes mcclellandii)、黄臀鹎(Pycnonotus xanthorrhous)、领雀嘴鹎(Spizixos semitorques)、黑头奇鹛(Heterophasia melanoleuca); 保护区动物地理区划处于东洋界华中区西部山地高原亚区,夏季鸟类区系以东洋界占明显优势,种类达83种,占物种数74.11%,古北界15种次之,广布种14种; 属国家Ⅱ级重点保护野生动物有7种,分别为褐冠鹃隼(Aviceda jerdoni)、蛇雕(Spilornis cheela)、凤头鹰(Accipiter trivirgatus)、红隼(Falco tinnunculus)、红角鸮(Otus sunia)、白冠长尾雉(Syrmaticus reevesii)、红腹锦鸡(Chrysolophus pictus); 属“国家保护的有益的或者有重要经济、科学研究价值的陆生野生动物”的67种,占物种数的59.82%; 白冠长尾雉被“中国生物多样性红色名录”列为濒危等级,10种被列为近危等级; 7种被列入CITES附录Ⅱ中; 6种中国特有种。

为加强野生动物及其生境的科学保护,2013年10月上、中旬,作者对湖南罗坪狩猎场野生动物资源进行了实地调查。根据实地调查结果、参考相关资料得知:狩猎场现已记录陆生脊椎动物154种,隶属4纲、24目、64科,其中两栖纲2目、5科、15种; 爬行纲3目、7科、20种; 鸟纲11目、32科、88种; 哺乳纲8目、20科、31种。湖南罗坪狩猎场在动物地理区划上属东洋界、华中区,介于西部山地高原亚区和东部丘陵平原亚区的过渡区域,动物区系组成以东洋界占明显优势,种类达102种,占该区域内陆生野生动物物种的66.88%; 广布种35种次之,古北界种类17种最少。项目区现已记录国家I级重点保护野生动物3种,即金雕(Aquila chrysaetos)、云豹(Neofelis nebulosa)和林麝(Moschus berezovskii yunguiensis); 国家II级重点保护野生动物23种,另有104种陆生脊椎动物属于“国家保护的有益的或者有重要经济、科学研究价值的陆生脊椎动物”,97种属于“湖南省地方重点保护野生动物”,7种中国特有种。