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目的建立利福平耐药基因rpoB高通量突变文库。方法通过同源重组和L5 attB噬菌体整合位点交换构建耻垢分枝杆菌ΔrpoB attB：：rpoB菌株；基于L5 attB质粒交换系统和抗性选择培养基，挑取48个克隆以验证质粒替换的效率；针对利福平耐药决定区（RRDR）区域内以每３个碱基为单位设计简并性引物，通过PCR扩增获得该区域81个碱基的全覆盖式突变文库；文库片段与载体进行无缝克隆转化到大肠杆菌中形成大肠杆菌突变文库；基于质粒交换原理，将突变质粒库转化至耻垢分枝杆菌ΔrpoB attB：：rpoB菌株中，替换原有L5 attB位点质粒，形成耻垢分枝杆菌突变文库；通过基因组提取、建库和高通量测序明确文库的基因型种类。结果与野生型rpoB基因（5 600 bp）相比，敲除株的扩增片段为2 200 bp，证明耻垢分枝杆菌ΔrpoB attB：：rpoB条件性敲除株构建成功；质粒替换的成功率为100%；大肠杆菌文库和耻垢分枝杆菌文库中单氨基酸突变类型均为540种，大肠杆菌文库多点突变5 301种，耻垢分枝杆菌文库多点突变853种，大肠杆菌文库和耻垢分枝杆菌文库相关系数为0.84。结论开发了一种针对分枝杆菌必需基因rpoB突变体文库的构建策略，成功建立了利福平耐药基因rpoB的突变文库。

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Water, as the primary volatile component in magmatic systems, has a significant impact on the formation and evolution of magma. The Tiaojishan Formation igneous rocks in the Liujiang Basin are significant products of Yanshanian volcanic activity. Although previous studies have extensively explored their geochemical characteristics, the water content of the magma in the Liujiang Basin during Yanshanian volcanic activity remains unclear. Melt inclusions, which capture the original magmatic information, serve as the most direct samples for determining the water content of magma. Based on geochemical analysis, this study quantitatively determines the water content in melt inclusions using laser Raman spectroscopy with standard samples. The results show that the lower tuff samples of the Tiaojishan Formation are characterized by high Si and Al contents, enrichment in LILEs, depletion in HFSEs, enrichment in LREEs, and depletion in HREEs. The water content in melt inclusions reveals a range of 0.99% to 4.98%, with an average of 2.62%. These characteristics jointly indicate the water-enriched acidic magmatic activity during the early Tiaojishan period in this area. Combining the water content of melt inclusions with the large-scale volcanic eruptions in the stage, this study suggests that high water content in the magma enhanced the eruptive dynamics of the magmatic system, making it a contributing factor to the large-scale volcanic eruption. [ABSTRACT FROM AUTHOR]

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Since the manufacturing process of aircraft panel parts resquires accurately extracting the machining allowance, this paper proposes a repair method of panel parts based on the measured point cloud data. First, a feature point detection algorithm based on normal difference is designed to extract the initial feature points of the reference panels. Second, the iterative shrinkage optimization model is established to perform shrinkage optimization on the initial feature points to obtain the final feature points of the reference panels. Finally, registration is performed according to the tooling positioning holes, and after the feature points of the reference panel are mapped to the panel to be repaired, feature points are connected in a certain order to obtain the processing path of the panel to be repaired. Processing is carried out according to the repairing path and the gap of the panels is detected after the assemble. Experimental result show that this method can accurately extract the processing path of the panel to be repaired and after processing verification, and the gap between the wall panels is less than 1.2 mm. [ABSTRACT FROM AUTHOR]

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In this paper, the effect of different concentrations of sucrose stress on color formation of the Staphylococcus xylosus was investigated. The results showed that the highest a * value and the best coloring effect similar to those of nitrite were observed after the addition of 0.05 g/mL sucrose to stress the S. xylosus. UV-Vis and electron spin resonance spectra analysis showed that production of coloring product Mb-NO was significantly enhanced after 0.05 g/mL sucrose stress. The growth curve, reactive oxygen content, cell cycle, nitric oxide synthase (NOS) activity, zeta potential, cell size, and protein composition of S. xylosus were investigated to reveal the mechanism of sucrose stress to enhance the coloring effect of the strain. The result showed that sucrose inhibited the growth of S. xylosus , which changed the physiological state by activating the oxidative stress response. The stress altered the rate of intracellular metabolism of S. xylosus by delaying the cell cycle and increasing cell surface zeta potential and cell particle size. These changes altered the protein composition of the cells and significantly enhanced the activity of intracellular NOS, which could improve the chromogenic ability of S. xylosus. This study will provide theoretical support for sucrose stress on S. xylosus to enhance its coloring effect, and sucrose stress for S. xylosus might be a promising biological alternative to nitrite in meat products. [ABSTRACT FROM AUTHOR]

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Copyright of Chinese Journal of Lung Cancer is the property of Chinese Journal of Lung Cancer and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In order to overcome the problems with the immediate roof control of gob-side entry retaining (GER) in a fully-mechanized top-coal caving longwall，a compound roof longwall and other soft cracked longwall，an elastic damage mechanics model of immediate roof above roadside backfill area (IRRBA) in GER was established，the stress distribution expression of IRRBA in GER was deduced，and the stress distribution evolution of IRRBA in GER was researched based on the space-time movement characteristics of IRRBA in GER. Then，the tensile stress depth and horizontal dislocation region calculation formulas of IRRBA in GER，and the corresponding evolution laws were obtained. Results show that the stress state of IRRBA in GER are associated with the main roof rotation angle and the immediate roof damage variable，and that the tensile stress depth decreases and the horizontal dislocation region increases with the increase of main roof rotation angle. Combined with the 3107 longwall engineering geological conditions of Xinyuan Colliery，the dynamic regional consolidation control countermeasures for IRRBA in GER were put forward. Field practice shows that the application of control countermeasures has achieved an expected effects for the subsidence of IRRBA and surrounding rock stability control in GER. [ABSTRACT FROM AUTHOR]