CPP(Cystein-rich polycomb-like protein or Tesmin/TOS1-like)protein family are one kind of transcription factors with fewer members and possess a conserved, Cys-rich CRC domains. CPP transcription factor family plays an important role in the plant development of reproductive tissue and cell division. In this study, a CPP-like family gene was cloned by RACE methods from Liriodendron tulipifera named LtTCX2 to explore the function in regulating flower development. The full length of LtTCX2 was 2 866 bp. LtTCX2 contained an open reading frame(ORF)of 2 424 bp, encoding 807 amino acids with two conserved TSO1-like CXC domains by bioinformatics analysis on NCBI site. The molecular weight of protein was 88 699.25 Da, isoelectric point was 5.83 and coefficient of instability was 62.38. LtTCX2 was predicted to be hydrophilic and non-transmembrane nucleoprotein without signal peptide. The amino acid homology sequence and phylogenetic analysis demonstrated that LtTCX2 had high homology with other CPP-like family proteins and was most closely related with NnTCX2 in Nelumbo nucifera and PeTCX2 in Populus euphratica. Real-time quantitative PCR showed that LtTCX2 gene was highly expressed in the leaf and hardly expressed in the sepal and petal. The tissue specific expression order from high to low was that the leaf, flower bud, pistil, stamen, stem, root, petal and sepal. These results suggested that LtTCX2 is a rather conservative gene and provide several help to the phylogenetic evolution of Liriodendron plants from the molecular biology level. [ABSTRACT FROM AUTHOR]