Objective To reform the teaching content of acupuncture and moxibustion for undergraduate of Nursing College and to evaluate the effectiveness of the teaching reform, so to provide constructive information for optimizing the curriculum. Methods To revise the teaching content of Traditional Chinese Medicine(TCM) acupuncture course based on the results of previous questionnaires, including reducing boring theoretical knowledge, increasing commonly used acupuncture techniques and clinical case analysis, organizing all undergraduates attending the TCM teaching in the Nursing College of Peking Union Medical College to fill out the “Acupuncture Course Content Questionnaire”in 2023. The content covers four aspects: students' basic cognition of acupuncture and moxibustion before the course, students' learning feedback,students'learning effectiveness after the course and students' suggestions for further optimization of acupuncture and moxibustion course. Results Totally 149 students participated in the questionnaire survey and 73.15% of them were interested in the acupuncture course. There were 62.42% of the students thought that the most difficult part of the course was the “Acupuncture Points”, and 71.81% of them complained the most constraining factor to the learning effectiveness was the difficulty of memorizing the meridians and acupoints. The most interesting part of the course was “Overview of Acupuncture Treatment and Acupuncture Treatment of Common Diseases”, which accounted for 44.3% of the students. Through the study, 88.59% of the students were willing to recommend acupuncture treatment to patients with indications.Students' suggestions for improvement of the acupuncture course was increase of classroom practice or learning by observation of operation videos. Conclusions The reformed acupuncture course highlights the practicability, improves the learning interest and subjective initiative, but the training of practice skill still needs to be strengthened. [ABSTRACT FROM AUTHOR]