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为阐明酚类化合物的抗氧化机理及利用其协同增效作用延缓油脂氧化，对油茶籽油中游离酚（free phenolics, FP）、酯化酚（esterified phenolics, EP）及不溶性结合酚（insoluble-bound phenolics, ISP）的提取工艺进行优化，并采用Schaal烘箱法研究不同形态酚类化合物单独或复配作用对油茶籽油氧化稳定性的影响，分析不同形态酚类化合物的互作关系。结果表明：FP的最优提取工艺条件为料液比1∶ 15、提取温度45 ℃、提取时间1.5 h、提取次数3次，EP的最优提取工艺条件为碱解液与乙酸乙酯体积比1∶ 1、萃取次数3次，ISP的最优提取工艺条件为NaOH浓度0.25 mol/L、料液比1∶ 3、碱解时间6 h、碱解温度45 ℃。在最优条件下，油茶籽油中FP、EP及ISP含量分别为（4343±2.05）mg/kg、（28.67±1.51）mg/kg及（65.87±1.34）mg/kg。不同形态酚类化合物均具有抑制氧化产物生成，增强油脂氧化稳定性的作用，其中EP+ISP的抗氧化性最强。各互作组分在油脂中均呈一定的拮抗作用，其中FP+EP+ISP的拮抗作用最强。 In order to clarify the antioxidant mechanism of phenolic compounds and use their synergistic effects to delay the oxidation of oils, the extraction processes of free phenolics (FP), esterified phenolics (EP) and insoluble-bound phenolics (ISP) were optimized, and the Schaal oven method was used to study the effect of different forms of phenolic compounds alone or in combination on the oxidative stability of oil-tea camellia seed oil, and the interaction between different forms of phenolic compounds was analyzed. The results showed that the optimal extraction process conditions of FP were obtained as follows: material-liquid ratio 1∶ 15, extraction temperature 45 ℃, extraction time 1.5 h, and extraction times 3. The optimal extraction process conditions of EP were obtained as follows: volume ratio of alkaline solution to ethyl acetate 1∶ 1, and extraction times 3. The optimal extraction process conditions of ISP were obtained as follows: alkaline hydrolysis time 6 h, alkaline hydrolysis temperature 45 ℃, NaOH concentration 0.25 mol/L and material-liquid ratio 1∶ 3. Under the optimal conditions, the contents of FP, EP and ISP in oil-tea camellia seed oil were (43.43±2.05) mg/kg, (28.67±1.51) mg/kg and (65.87±134) mg/kg, respectively, and the different forms of phenolic compounds had the effect of inhibiting the formation of oxidation products and enhancing the oxidative stability of the oil. The combination of EP and ISP had the strongest antioxidation activity. Each of the interacting components had a certain antagonistic effect in the oil, and the antagonistic effect of the combination of FP, EP and ISP was the strongest.

Ovarian cancer is a malignant tumor of the female reproductive system with the highest mortality, and involves the aberrant regulation of multiple molecular signaling pathways. As a highly conserved molecular pathway, Wnt signaling pathway plays an important role in embryonic development, tissue homeostasis, and tumorigenesis. Wnt signaling pathway includes canonical Wnt/β -catenin pathway and non-canonical pathway, and the latter mainly includes Wnt/planar cell polarity (Wnt/PCP) pathway and Wnt/Ca2+ pathway. Previous studies have mainly focused on the relationship between the canonical Wnt pathway and tumor progression. Recently, the non-canonical Wnt pathway has gradually received attention, and related researches have enriched the understanding of the non-canonical Wnt pathway in physiological and pathological processes such as tissue development and tumorigenesis. The existing studies suggest that the nonclassical Wnt pathway is abnormally regulated in ovarian cancer and is closely related to the staging and prognosis of ovarian cancer. Non-classical Wnt pathway plays an important role in many biological processes such as proliferation, migration and invasion of ovarian cancer cells, and the changes of this pathway are also related to chemotherapy resistance of ovarian cancer. This article reviews the role of the non-canonical Wnt pathway in ovarian cancer, and discusses the research progress of targeted therapy based on the non-canonical Wnt pathway, aiming to provide new ideas for the development of novel targeted drugs. [ABSTRACT FROM AUTHOR]

Suriana maritima is a coastal ornamental plant species distributing only on the South China Sea Islands in China, at present. In order to understand the ecological and biological characteristics of S. maritima, we investigated the morphological and anatomical structure of stem and leaf, the antioxidant enzyme activity and osmotic adjustments contents in leaf of S. maritima, and nutrient element contents in leaf and soil of S. maritima growing on the Paracel Islands, by field investigation and sampling for analysis. The results were as follows:(1)S. maritima had small and thick leaf, well-developed palisade tissue, and low stomatal density(8.64 n·mm-2), which make it easy to maintain water in the body and suitable for drought and high saline-alkali environments. The chlorophyll content(0.76 mg·g-1)of the leaf was low, the total antioxidant capacity(589.50 U·g-1)and proline content(1 123.64 μg·g-1)were high, indicating that S. maritima had high photosynthetic utilization efficiency and strong antioxidant capacity.(2)Though the nutrient in natural growing soil was poor, the contents of organic carbon, nitrogen and phosphorus in leaf of S. maritima were relatively high(490.27, 18.10, and 3.81 g·kg-1, respectively), indicating a high nutrient utilization efficiency and good adaptability of S. maritima to poor soil. In conclusion, S. maritima has a good adaptability to the environment of strong radiation, drought, high saline-alkali and poor soil on tropical coral islands, and can be used as a tool species for revegetation and landscaping on tropical coral islands. The results provide a theoretical basis for its protection, development and utilization. [ABSTRACT FROM AUTHOR]

In order to explore the ecophysiological adaptability of Hernandia nymphaeifolia to the tropical coral islands with harsh conditions of seasonal drought, strong light, and saline-alkali soil, we investigated the morphological and anatomical structures, antioxidant capacity, content of osmotic adjustments, and photosynthetic pigments in leaves of H. nymphaeifolia individuals that grown on the experimental nursery in Wenchang City, and that transplanted to the tropical coral islands, Hainan Province. The results were as follows:(1)Compared to H. nymphaeifolia plants grown in the experimental nursery, those plants transplanted to the tropical islands had lower specific leaf area(by 33%)and stomatal density(by 15%), thicker leaf(by 36%), more developed spongy tissue and more closely arranged palisade tissue(by 24% and 80%, respectively), which enable it to make more efficient use of light and water resources, to improve photosynthesis and to reduce transpiration, in the coral island environment.(2)After transplanted to the coral islands, the chlorophyll content of H. nymphaeifolia significantly decreased(by 86%), while proline content, superoxide dismutase, catalase, and peroxidase activity significantly increased(by 130%, 615%, 209% and 369%, respectively), which can protect its photosynthetic system and avoid the damage of plant cell membrane permeability caused by the adverse environment of coral islands. Our results indicated that H. nymphaeifolia had good ecophysiological adaptability to the environment of drought, strong light and saline-alkali soil in tropical coral islands. Therefore, H. nymphaeifolia can be used as a tool species for vegetation restoration on tropical coral islands and coastal areas. [ABSTRACT FROM AUTHOR]