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6. Identification of the mitochondrially encoded subunit 6 of F1FO ATPase in Trypanosoma brucei

Author

Škodová-Sveráková, Ingrid, Horváth, Anton, and Maslov, Dmitri A

Subjects
Biochemistry and Cell Biology, Biological Sciences, Genetics, Vector-Borne Diseases, Infectious Diseases, Denaturing Gradient Gel Electrophoresis, Electrophoresis, Gel, Two-Dimensional, Isotope Labeling, Mitochondrial Proteins, Protein Subunits, Proton-Translocating ATPases, Sequence Analysis, Protein, Trypanosoma brucei brucei, F(1)F(O) ATPase, Mitochondrial translation, RNA editing, Subunit 6, Trypanosoma brucei, Agricultural and Veterinary Sciences, Medical and Health Sciences, Mycology & Parasitology, Biochemistry and cell biology, Microbiology, Medical microbiology
Abstract

Kinetoplast maxicircle DNA of trypanosomatids encodes eighteen proteins. RNA editing is required to confer translatability to mRNA for twelve of these. Sequence conservation of the predicted hydrophobic polypeptides indicates that they represent functional components of the respiratory chain. Yet, so far only two of those, cytochrome c oxidase subunit I and apocytochrome b of cytochrome c reductase, have been identified with biochemical methods. Here we report on identification of A6 subunit of F1FO ATPase encoded by a pan-edited mRNA in Trypanosoma brucei. The polypeptide was present among the (35)S-labeled mitochondrial translation products characterized by anomalous migration in denaturing 2D gels. It was identified as an ATPase subunit by co-migration with this complex in Blue Native 2D gels. A partial N-terminal sequence of the corresponding polypeptide present in the gel-purified ATPase complex from Leishmania tarentolae was consistent with the predicted A6 sequence.

Published
2015

7. Leishmania guyanensis M4147 as a new LRV1-bearing model parasite: Phosphatidate phosphatase 2-like protein controls cell cycle progression and intracellular lipid content

Author

Zakharova, Alexandra, primary, Albanaz, Amanda T. S., additional, Opperdoes, Fred R., additional, Škodová-Sveráková, Ingrid, additional, Zagirova, Diana, additional, Saura, Andreu, additional, Chmelová, Lˇubomíra, additional, Gerasimov, Evgeny S., additional, Leštinová, Tereza, additional, Bečvář, Tomáš, additional, Sádlová, Jovana, additional, Volf, Petr, additional, Lukeš, Julius, additional, Horváth, Anton, additional, Butenko, Anzhelika, additional, and Yurchenko, Vyacheslav, additional

Published
2022
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