Search

The concept of low carbon education is one solution to provide knowledge to students related to low carbon behavior. The purpose of this paper is providing an extensive bibliometric literature review on 'low carbon education'. Articles found by Publishing or Perish (PoP) software with the Google Scholar database. There were 55 out of 97 articles found from Google Scholar data base ranging from 2014 to 2019 analyzed in this study. The chosen references were then managed using a referencing manager software namely Zotero. After managing the database, this study classified and visualized it using VOSviewer software. Overall, this review provides an appropriate reference point for further research on 'low carbon education'.

Copyright of Croatian Journal of Fisheries is the property of Sciendo and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Heavy rainfall/flood (HRF) cyclones contribute close to two-thirds of the total rainfall PT in both parts of Malaysia [peninsular Malaysia (M) and west Borneo (B)]. Judging by the rainfall variance produced by these cyclones and its correlation (~0.9) with the interannual PT variation, this variation is caused primarily by HRF cyclones through two factors: 1) their westward propagation properties and 2) their rain-producing efficiency. The former is regulated by the change of the cyclonic shear flow around the near-equator trough, while the latter is determined by the change of the convergence of water vapor flux toward tropical Southeast Asia. During November-December of cold (warm) ENSO phases, the westward propagation of the cyclone's parent cold surge vortices (CSVs) from the Philippine (P) vicinity (Borneo) to peninsular Malaysia CSVPMs (CSVBMs) and intensified (weakened) convergence of water vapor flux toward tropical South/Southeast Asia act to enhance (reduce) the rain-producing efficiency of HRFPM (HRFBM) cyclones. During winter cold (warm) phases, the deepening (filling) of the near-equator trough crossing west Borneo allows some CSVs formed/trapped in Borneo CSVBBs to develop into HRFBB (HRFBBM) cyclones (to propagate westward to peninsular Malaysia). The rain-producing efficiency of HRFBB and HRFBBM cyclones is also increased (reduced) by the intensified (weakened) convergence of water flux toward tropical South/Southeast Asia. Interannual variations of both PT(M) and PT(B) caused by the impacts of the circulation pattern changes on occurrences of HRFPM and HRFBB/HRFBBM cyclones, respectively, and their rain-producing efficiency may pose a new challenge to simulate the weather-climate relationship in climate modeling. [ABSTRACT FROM AUTHOR]

Malaysia is geographically separated into Peninsular Malaysia and west Borneo. The rainfall maximum in the former region occurs during November-December, whereas that in the latter region occurs during December-February. This difference of maximum rainfall period indicates that the formation mechanism is different for the rainfall centers in these two parts of Malaysia. Since rainfall is primarily produced by severe weather systems, the formation of a climatological rainfall center is explored through synoptic activity and the rainfall amount of this center is estimated through contributions by rain-producing disturbances. The major cause of the rainfall maximum of Peninsular Malaysia is cold surge vortices (CSVs) and heavy rainfall/flood (HRF) events propagating from the Philippine area and Borneo. In contrast, the major cause of the rainfall maximum of Borneo is these rain-producing disturbances trapped in Borneo. Disturbances of the former group are formed by the cold surge flows of the Philippine Sea type, whereas disturbances of the latter group are formed by cold surge flows of the South China Sea (SCS) type. The population of HRF events is about one-fourth of the rain-producing disturbances in both Peninsular Malaysia and Borneo, but they produce less than ~60% rainfall for these two regions. It is revealed from the synoptic and dynamic analyses that the major Borneo rain-producing disturbances propagate westward before December by strong tropical easterlies, but they are trapped after December by strong northeasterlies of the SCS-type cold surge flow. [ABSTRACT FROM AUTHOR]

In this study, we quantified carbonaceous PM2.5 in Malaysia through annual observations of PM2.5, focusing on organic compounds derived from biomass burning. We determined organic carbon (OC), elemental carbon and concentrations of solvent-extractable organic compounds (biomarkers derived from biomass burning sources and n-alkanes). We observed seasonal variations in the concentrations of pyrolyzed OC (OP), levoglucosan (LG), mannosan (MN), galactosan, syringaldehyde, vanillic acid (VA) and cholesterol. The average concentrations of OP, LG, MN, galactosan, VA and cholesterol were higher during the southwestern monsoon season (June-September) than during the northeastern monsoon season (December-March), and these differences were statistically significant. Conversely, the syringaldehyde concentration during the southwestern monsoon season was lower. The PM2.5 OP = OC4 mass ratio allowed distinguishing the seven samples, which have been affected by the Indonesian peatland fires (IPFs). In addition, we observed significant differences in the concentrations between the Indonesian peatland fire (IPF) and other samples of many chemical species. Thus, the chemical characteristics of PM2.5 in Malaysia appeared to be significantly influenced by IPFs during the southwestern monsoon season. Furthermore, we evaluated two indicators, the vanillic acid = syringic acid (VA= SA) and LG =MN mass ratios, which have been suggested as indicators of IPFs. The LG =MN mass ratio ranged from 14 to 22 in the IPF samples and from 11 to 31 in the other samples. Thus, the respective variation ranges partially overlapped. Consequently, this ratio did not satisfactorily reflect the effects of IPFs in Malaysia. In contrast, the VA= SA mass ratio may serve as a good indicator, since it significantly differed between the IPF and other samples. However, the OP = OC4 mass ratio provided more remarkable differences than the VA= SA mass ratio, offering an even better indicator. Finally, we extracted biomass burning emissions' sources such as IPF, softwood/hardwood burning and meat cooking through varimax-rotated principal component analysis. [ABSTRACT FROM AUTHOR]

Assessment of a society's environmental load is one of the important tasks when creating a sustainable society. The authors of this paper assessed CO2 emissions and forest resource sustainability for the housing construction taking place in Malaysia using an Input–Output analysis. Common housing types such as apartments and terraced houses were assessed. The findings showed that the main sources of CO2 emissions during the construction phase were generated in the building components, such as building structures and foundations. The forest resource sustainability will be sustained under certain conditions, even if timber housing construction is to be promoted. This paper also discusses the methods for both CO2 reductions and forest resource sustainability. [ABSTRACT FROM PUBLISHER]

Copyright of Environmental & Social Management Journal / Revista de Gestão Social e Ambiental is the property of Environmental & Social Management Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

VX is a strong nerve-gas agent. In this paper, murder or injury of human made by VX is reported. First use of VX against human was made by Aum Shimrikyo in 1994 and 1995. More recently, VX was used in the assassination of Mr. Kim Jong-Nam in Kuala Lumpur, Malaysia, on 13 February 2017. In this review article, the use of VX against human is discussed together and described in detail. [ABSTRACT FROM AUTHOR]

Background: Colorectal cancer (CRC) is a major cause of cancer-related mortality worldwide. It is the second leading cause of cancer death in men and women in Malaysia and poses a major burden on society. Aims: To determine the overall survival rate of patients diagnosed with CRC and factors contributing to survival. Methods: Data were obtained from the Malaysia National Cancer Registry. All patients with CRC were identified, and a total of 15,515 patients were screened. A total of 5,675 CRC patients were included from January 1, 2012, to December 31, 2016. Sex, age groups, ethnic groups, stage at diagnosis, cancer sites, and status of treatment received were analysed. The Kaplan--Meier analysis was performed to estimate the 1-, 3-, and 5-year survival of CRC. The log-rank test was conducted to compare the survival between sex, age groups, ethnic groups, stage at diagnosis, cancer sites, and status of treatment received. Multiple Cox regression was conducted to determine the risk of CRC death. Results: Of 5,675, a total of 2,055 had died, 3,534 were censored, and another 86 were still alive within 5 years of CRC diagnosis. The 1-, 3-, and 5-year survival rates were 68.5%, 34.7%, and 18.4%, respectively with a median survival time of 24 months. Significant differences in survival rates of CRC were observed between age groups (p < 0.001), ethnic groups (p < 0.001), stages at diagnosis (p < 0.001), treatment status (p = 0.003), and treatment modalities (p < 0.001). No significant difference was observed in survival rates of CRC between sex (p = 0.235) and cancer sites (p = 0.410). Those who were 80 years old and above were found to be at higher risk of CRC death compared to those below 80 years old (adjusted hazard ratio (HR): 1.24, 95% CI 1.14-1.36). The risk of CRC death was also found four times higher among those with stage IV compared to those with stage 0 (adjusted HR: 4.28, 95% CI 3.26-5.62). Conclusion: In general, Malaysian patients with CRC had low survival rates. National health policies should focus on enhancing awareness of CRC, encouraging early screening, and developing strategies for early detection and management to reduce CRC-associated mortality. [ABSTRACT FROM AUTHOR]

To identify potential limitations that may impact the quality and efficiency of end-of-life vehicle (ELV) recycling and to provide solutions for effective management, this study investigates the current ELV recycling practice and the quality assessment systems around it in Malaysia by employing a mixed-methods approach. The approach encompasses qualitative and quantitative research alongside reviewing pertinent literature. The findings reveal that ELV recycling and its accompanying quality assessment systems in Malaysia are restricted and confront many critical challenges. The challenges include the lack of appropriate processes for recycling to maximize the material recovery, and the quality and reliability of the components put into circular economy practice. As a practical solution, a stakeholder-centric framework is proposed that allows better management of ELV waste from transportation and other related sectors. This framework enhances the efficiency and quality of ELV recycling practices in Malaysia and helps assess the quality of components recovered from ELVs. [ABSTRACT FROM AUTHOR]

The CRB (coconut rhinoceros beetle) haplotype was classified into CRB-S and CRB-G, based on the presence of single nucleotide polymorphisms (SNPs) in the mitochondrial cox1 gene. Mitochondrial genomes (mitogenomes) are the most widely used genetic resources for molecular evolution, phylogenetics, and population genetics in relation to insects. This study presents the mitogenome CRB-G and CRB-S which were collected in Johor, Malaysia. The mitogenome of CRB-G collected from oil palm plantations in 2020 and 2021, and wild coconut palms in 2021 was 15,315 bp, 15,475 bp, and 17,275 bp, respectively. The CRB-S was discovered in coconut and oil palms in 2021, and its mitogenome was 15,484 bp and 17,142 bp, respectively. All the mitogenomes have 37 genes with more than 99% nucleotide sequence homology, except the CRB-G haplotype collected from oil palm in 2021 with 89.24% nucleotide sequence homology. The mitogenome of Johor CRBs was variable in the natural population due to its elevated mutation rate. Substitutions and indels in cox1, cox2, nad2 and atp6 genes were able to distinguish the Johor CRBs into two haplotypes. The mitogenome data generated in the present study may provide baseline information to study the infection and relationship between the two haplotypes of Johor CRB and OrNV in the field. This study is the first report on the mitogenomes of mixed haplotypes of CRB in the field. [ABSTRACT FROM AUTHOR]

Acid sulfate soil characterized by pyrite (FeS2) which produces high acidity (soil pH < 3.5) and release high amount of Al3+ and Fe2+. Application of 4 t ha-1 Ground Magnesium Limestone (GML), is a common rate used for acid sulfate soil by the rice farmers in Malaysia. Therefore, this study was conducted to evaluate the integral effect of ground magnesium limestone (GML) and calcium silicate and to determine the optimal combination on acid sulfate soils in Malaysia. The acid sulfate soils were incubated under the submerged condition for 120 days with GML (0, 2, 4, 6 t ha-1) in combination with calcium silicate (0, 1, 2, 3 t ha-1) arranged in a Completely Randomized Design (CRD). The soil was sampled after 30, 60, 90 and 120 days of incubation and analyzed for soil pH, exchangeable Al, Ca, Mg, K and available Si. A total of 2 out of 16 combinations met the desired soil requirement for rice cultivation. The desired chemical soil characteristics for rice cultivation are soil pH > 4, exchangeable Al < 2 cmolc Kg-1, exchangeable Ca > 2 cmolc kg-1, exchangeable Mg > 1 cmolc kg-1 and Si content > 43 mg kg-1. The combinations are i) 2 t ha-1 calcium silicate + 2 t ha-1 GML, and ii) 3 t ha-1 calcium silicate + 2 t ha-1 GML, respectively. These combination rates met the desired requirement of soil chemical characteristics for rice cultivation. Soil acidity was reduced by a gradual release of Ca2+ and SiO32- from calcium silicate continuously filling the exchange sites and reducing the potential of extra (free) H+ availability in the soil system. Combination of calcium silicate and GML, shows the ameliorative effect with; i) release of Ca, ii) binding of Al3+ making it inert Al-hydroxides and, iii) bind H+ to produce water molecules. [ABSTRACT FROM AUTHOR]

Background: The number of people living with dementia in Malaysia is expected to increase with the nation's growing elderly population and increased lifespan. The lack of public awareness of dementia is partly compounded by low personal health literacy, while scarce research on local patient awareness further impacts the execution of optimised healthcare services in Malaysia. Patients with chronic disease have an elevated risk of developing mild cognitive impairment (MCI). This study aimed to assess the level of awareness of basic knowledge on dementia among the elderly, especially those at risk of developing mild cognitive impairment and its associated factors. Methods: A total of 207 elderly patients aged 60 years and above with chronic diseases attending a university-based primary care clinic were recruited via a systematic randomised sampling method from the clinic patient attendance registry. Respondents were assessed using self-administered online questionnaires distributed via mobile devices. The questionnaire assessed awareness, i.e. ability to correctly answer a self-reported questionnaire on basic dementia knowledge; (adapted from Northern Ireland Life and Times Survey 2010), risk of MCI; (using Towards Useful Aging (TUA)-WELLNESS screening questionnaire) and help-seeking behaviour. Bivariate analysis was used to determine factors associated with dementia awareness. Results: The response rate was 77.1%, with the majority of participants were females, Chinese and had secondary school education. 39.1% of participants were categorised as high risk of developing MCI. The majority (92.8%) had low dementia awareness and had never shared their concerns regarding dementia (93.2%) nor had any discussion (87.0%) on cognitive impairment with their physicians. Three factors had an association with total dementia awareness score, i.e., younger age group, higher risk of MCI and presence of cardiovascular diseases have significantly lower awareness score (p < 0.05). Conclusion: Awareness of dementia is low among elderly patients with potentially high risk of developing MCI. Efforts to improve awareness on dementia should focus on primary care doctors engaging with at-risk elderly patients to initiate discussion regarding dementia risk while managing modifiable risk factors i.e. hypertension control, diabetes, dyslipidaemia and obesity. [ABSTRACT FROM AUTHOR]

Small coastal demersal sharks form a major proportion of the sharks landed in Malaysia. However, little is known about their feeding ecology and reproduction. This study sought to elucidate the dietary patterns, role of ontogeny in prey consumption, and reproductive biology of four dominant small demersal shark species in Malaysian waters: the Hasselt's bamboo shark, Chiloscyllium hasseltii; brownbanded bamboo shark, C. punctatum; spadenose shark, Scoliodon laticaudus; and Pacific spadenose shark, S. macrorhynchos. Dietary analyses revealed a high overlap in prey taxa consumed; clear resource partitioning among co-occurring species based on the percentage Prey-specific Index of Relative Importance (%PSIRI), with higher fish %PSIRI for Chiloscyllium hasseltii, higher cephalopod %PSIRI for C. punctatum, and higher crustacean %PSIRI for both Scoliodon species; and an ontogenetic diet shift, seen through changes in prey size. Based on the examination of reproductive organs, the results showed larger sizes at maturity for males compared to females for all four species; no obvious reproductive cycles, based on hepatosomatic and gonadosomatic indices for all species; female bias in the sex ratio of the embryos of Scoliodon species; and increased reproductive output (number of eggs or embryos and size of eggs) with larger female size for C. hasseltii and Scoliodon species. The partitioning of food resources minimizes direct competition for food and supports coexistence within shared coastal habitats. The reproductive strategies of these small coastal sharks appear to be favorable for supporting short-term population productivity; although a reduction in fishing pressure, especially from bottom trawlers, is essential for the long-term sustainable use of these sharks. [ABSTRACT FROM AUTHOR]

This research paper investigates the traditional Malay silver craft that has existed for two centuries, reflecting the community's culture and heritage. The Srivijaya Kingdom and British colonial rule historically influenced this Malay culture's silver craft, encapsulating beliefs, dreams, and resilience. Originating in Kelantan, Malaysia, the Malay silver craft industry flourished, but recent studies reveal its decline and potential extinction. This paper's primary objective is to comprehensively identify and propose strategies for integrating sustainable practices of silver craft, aligning with the UN's Sustainable Development Goals (SDGs), focusing on sustainable industrialization and cultural heritage preservation. The research seeks to preserve and revitalize the craft industry for community benefit, emphasizing its socio-economic impact, potential for job creation, income generation, tourism, and intangible heritage preservation. The paper conducts a literature review, visual research, and focus group discussions employing anthropology, art history, and community development. This research emphasizes the importance of sustaining cultural heritage, fostering community resilience, and empowerment. Influenced by their cultural beliefs, environment, looking at nature, and the natural world, the fine silver craft undergoes artistic expression, subsequently fostering social cohesion and interaction. Following the quadruple helix concept, the Malay silver craft industry revival engaged through collaboration among local artisans, government agencies, community organizations, and academicians. By utilizing thematic analysis based on focus group discussion, this research thoroughly analyses the sustainability of the Malay silver craft domain, interpreting key thematic codes that underpin its ecological and socio-cultural significance within the framework of local craft in Malaysia. [ABSTRACT FROM AUTHOR]

In recent decades, significant changes have occurred in the consumer lifestyle and energy consumption structure. In order to foster a more holistic understanding of how residential consumers use energy in their everyday life and how it affects energy efficiency, individual data about energy-related behaviour in six primary areas, namely, housing, mobility, diet, consumption, leisure activities, and information, were studied. Specifically, the objective of this study is to investigate energy efficiency behaviour based on residential consumers' lifestyles. An exploratory pilot study with a total of 50 respondents was carried out in month of July. Data were collected from residential consumers from six states in Peninsular Malaysia and were analysed using the Partial Least Square Structural Equation Modelling approach. The result showed that consumption, housing, and information are the dominant direct contributors to energy efficiency. By understanding these lifestyles, researchers and policymakers can better understand consumer energy consumption behaviour and design targeted interventions to encourage energy efficiency and conservation. The paper highlights the need for further research on consumer lifestyles and their contribution to energy efficiency. It also provides findings from this study that was used to refine the research tools for future research. [ABSTRACT FROM AUTHOR]

A pronunciation dictionary (PD) is one of the components in an Automatic Speech Recognition (ASR) system, a system that is used to convert speech to text. The dictionary consists of word-phoneme pairs that map sound units to phonetic units for modelling and predictions. Research has shown that words can be transcribed to phoneme sequences using grapheme-to-phoneme (G2P) models, which could expedite building PDs. The G2P models can be developed by training seed PD data using statistical approaches requiring large amounts of data. Consequently, building PD for under-resourced languages is a great challenge due to poor grapheme and phoneme systems in these languages. Moreover, some PDs must include pronunciation variants, including regional accents that native speakers practice. For example, recent work on a pronunciation dictionary for an ASR in Iban, an under-resourced language from Malaysia, was built through a bootstrapping G2P method. However, the current Iban pronunciation dictionary has yet to include pronunciation variants that the Ibans practice. Researchers have done recent studies on Iban pronunciation variants, but no computational methods for generating the variants are available yet. Thus, this paper reviews G2P algorithms and processes we would use to develop pronunciation variants automatically. Specifically, we discuss data-driven techniques such as CRF, JSM, and JMM. These methods were used to build PDs for Thai, Arabic, Tunisian, and Swiss-German languages. Moreover, this paper also highlights the importance of pronunciation variants and how they can affect ASR performance. [ABSTRACT FROM AUTHOR]

Simple Summary: One-third of shark and ray species are threatened due to overfishing, but a lack of information on each species makes conservation decisions difficult. To address this issue, we conducted a study to identify the different species of sharks and rays in Malaysian waters using DNA barcoding of the CO1 gene, which is akin to DNA fingerprinting for species. We collected 175 individuals between June 2015 and June 2022, randomly selecting up to six specimens from each species. We successfully generated DNA barcodes for 67 species, belonging to 44 genera, 20 families, and 11 orders. Accurate species identification will improve species-specific catch landing data and accelerate the identification of use and illegal trade in Malaysia. The data provided in this article are partial fragments of the Cytochrome c oxidase subunit 1 mitochondrial gene (CO1) sequences of 175 tissues sampled from sharks and batoids collected from Malaysian waters, from June 2015 to June 2022. The barcoding was done randomly for six specimens from each species, so as to authenticate the code. We generated barcodes for 67 different species in 20 families and 11 orders. DNA was extracted from the tissue samples following the Chelex protocols and amplified by polymerase chain reaction (PCR) using the barcoding universal primers FishF2 and FishR2. A total of 654 base pairs (bp) of barcode CO1 gene from 175 samples were sequenced and analysed. The genetic sequences were blasted into the NCBI GenBank and Barcode of Life Data System (BOLD). A review of the blast search confirmed that there were 68 valid species of sharks and batoids that occurred in Malaysian waters. We provided the data of the COI gene mid-point rooting phylogenetic relation trees and analysed the genetic distances among infra-class and order, intra-species, inter-specific, inter-genus, inter-familiar, and inter-order. We confirmed the addition of Squalus edmundsi, Carcharhinus amboinensis, Alopias superciliosus, and Myliobatis hamlyni as new records for Malaysia. The establishment of a comprehensive CO1 database for sharks and batoids will help facilitate the rapid monitoring and assessment of elasmobranch fisheries using environmental DNA methods. [ABSTRACT FROM AUTHOR]

The two varieties of mangosteen (Garcinia mangostana L.) cultivated in Malaysia are known as Manggis and Mesta. The latter is preferred for its flavor, texture, and seedlessness. Here, we report a complete plastome (156,580 bp) of the Mesta variety that was obtained through a hybrid assembly approach using PacBio and Illumina sequencing reads. It encompasses a large single-copy (LSC) region (85,383 bp) and a small single-copy (SSC) region (17,137 bp) that are separated by 27,230 bp of inverted repeat (IR) regions at both ends. The plastome comprises 128 genes, namely, 83 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. The plastome of the Manggis variety (156,582 bp) obtained from reference-guided assembly of Illumina reads was found to be nearly identical to Mesta except for two indels and the presence of a single-nucleotide polymorphism (SNP). Comparative analyses with other publicly available Garcinia plastomes, including G. anomala, G. gummi-gutta, G. mangostana var. Thailand, G. oblongifolia, G. paucinervis, and G. pedunculata, found that the gene content, gene order, and gene orientation were highly conserved among the Garcinia species. Phylogenomic analysis divided the six Garcinia plastomes into three groups, with the Mesta and Manggis varieties clustered closer to G. anomala, G. gummi-gutta, and G. oblongifolia, while the Thailand variety clustered with G. pedunculata in another group. These findings serve as future references for the identification of species or varieties and facilitate phylogenomic analysis of lineages from the Garcinia genus to better understand their evolutionary history. [ABSTRACT FROM AUTHOR]

Eurycoma longifolia plant, the so called Tongkat Ali in Malaysia, is a well grown prominent tree in all Southeast Asia. It is well known among traditional medicine practitioners as a curative plant for many diseases and health conditions. The major quassinoid from the plant is eurycomanone, which exhibits many prominent effects on various cancer cell lines. Numerous studies have shown that eurycomanone inhibits cancerous cell growth and encourages cell death both in vitro and in vivo test. Even though analyses of safety and toxicity have been conducted, there is still a substantial knowledge barrier when it comes to providing a scientific foundation for the molecular mechanism as well as intervention strategy in the living people cancer cell. In a way to offer adequate baseline data for future investigations based on molecular mechanism and intervention, the present work seeks to review the researches conducted to date on this herbal plant. [ABSTRACT FROM AUTHOR]

Studies of the effects of elevation and associated habitat changes on biodiversity have a rich history in conservation biology and have seen a resurgence of interest in recent decades. Mountainous regions are particularly important because they harbour endemic species and are relatively spared from anthropogenic effects. The rather drastic temperature decrease as elevation increases in tropical regions has generated more research on the effects of elevation, especially since global warming could negatively impact the biodiversity of tropical forest mountainous species. Fireflies, especially the solitary species, represent a challenge to work with because though they are biologically diverse, they typically occur at low densities and have rarely been studied across elevations. Many are habitat specialists and have limited dispersal abilities. Firefly diversity changes on five mountains located on the main mountain range of Peninsular Malaysia, which is highly representative of five major elevational forest types, were assessed. Fireflies were restricted to a certain elevational range of mountains, and the turnover of species was significant among forest types across elevations. The forest type and canopy closure were the main characteristics affecting species diversity, although other habitat characteristics may be significant. The ability to reveal any possible associations was limited, as strong statistical associations were not possible due to their low abundance and difficulty in detecting their presence. The firefly species occurrence across elevations is restricted, and habitat loss could pose a risk to lower-elevation species, while global warming could be a threat to high-elevation species. [ABSTRACT FROM AUTHOR]

Various studies on population genetics in the Indo-Pacific that include the Coral Triangle region have revealed phylogeographic patterns in marine species populations. However, little is known about the population structure and connectivity pattern among the coral reefs in four seas surrounding Malaysia: the Strait of Malacca (Andaman Sea), the South China Sea, the Sulu Sea, and the Sulawesi Sea. In view of this, we examined the genetic population structure of two invertebrate species: the blue sea star (Linckia laevigata) and the boring clam (Tridacna crocea) from seven localities across Malaysia, including Pulau Layang-Layang in the Spratly Islands. Additional samples of L. laevigata were obtained from the Davao Gulf, the Philippines. The analyses were based on partial mitochondrial cytochrome oxidase I sequences of L. laevigata and T. crocea. Populations of L. laevigata and T. crocea showed departure from neutrality, indicating selection or population expansion. However, a mismatch analysis suggested population expansion. Lack of genetic population structure in L. laevigata (ΦST = − 0.001, P = 0.399) implies high connectivity among the sampled reef sites. There was subtle genetic structuring in populations of T. crocea (ΦST = 0.032, P = 0.009), but AMOVA did not detect any structure in the a priori groupings. The genetic population structure and scale in connectivity are most likely attributed to the life history traits of the sea star and giant clam species, and also to the geological history and seasonal current patterns of the region. [ABSTRACT FROM AUTHOR]

The Malaysian national school curriculum currently lacks resources and tools to enforce CPR education. The aim of this study was to investigate the efficacy of a compression-only cardiopulmonary resuscitation and automated external defibrillator course among primary school students to increase their knowledge and technical skills and improve their attitudes. A quasi-experimental study was conducted using a pre–post non-equivalent design involving 38 students aged 10–12. Cardiopulmonary resuscitation (CPR) and automated external defibrillator (AED) knowledge, technical skills, and attitude towards CPR were assessed in a post test with three-month follow-up. Results of the MANOVA analysis showed significant differences in the level of knowledge (F = 10.29, p < 0.001) and attitude (F = 13.87, p < 0.001) based on the students' age group at the time of the post test. The proportion of students who passed the technical skills component differed significantly by age (χ2 = 12.12; p = 0.002) and BMI (χ2 = 6.34; p = 0.041). No significant decay was reported in the total mean scores for knowledge, technical skills, and attitude (F = 0.727, p = 0.54) at 3-month follow-up. The course helped students perform CPR and utilize AED effectively while promoting a positive attitude with up to 3 months of retention, demonstrating the feasibility of extending the course within the Malaysian primary school curriculum. [ABSTRACT FROM AUTHOR]

Symptoms of leaf blight, stem canker, and pod rot were observed on T. cacao during a series of samplings conducted in several states of Malaysia from September 2018 to March 2019. The identity of the pathogen that was responsible for the diseases was determined using morphological characteristics, DNA sequences, and phylogenetic analyses of multiple genes, namely, internal transcribed spacer (ITS), elongation translation factor 1-alpha (tef1-α), β-tubulin (tub2), and RNA polymerase subunit II (rpb2). A total of 57 isolates recovered from diseased leaves of T. cacao (13 isolates), stems (20 isolates), and pods (24 isolates) showed morphological features that resembled Lasiodiplodia sp. The identity of the isolates was further determined up to the species level by comparing DNA sequences and phylogenetic analyses of multiple genes. The phylogenetic analysis of the combined dataset of ITS, tef1-α, tub2, and rpb2 elucidated that all of the isolates obtained were Lasiodiplodia theobromae as supported by 97% bootstrap value. The results of pathogenicity tests revealed L. theobromae as the causal pathogen of leaf blight, stem canker, and pod rot of T. cacao. [ABSTRACT FROM AUTHOR]

Simple Summary: Groupers are one of Asia's most valuable marine fish. The strong market demand has driven the expansion of grouper farming. However, with the intense farming practices, the farm rearing system was exposed, thus increasing the risk of infectious diseases. The present study was conducted to compare the use of different fatty acid immunostimulants on the survival, growth, and immune response of hybrid grouper infected with Vibrio vulnificus. Our results showed that the oleic acid formulated diet gives the highest fish survival rate and growth rate compared to the other fatty acid formulated diets and control diet after six weeks of feeding trial and one week of the bacterial challenge. Moreover, fish supplemented with an oleic acid diet showed significantly increased immune responses after being infected with V. vulnificus. Aquaculture has been expanding in Malaysia due to the increased demand for fish products. In addition, aquaculture faces challenges in maintaining feed suitability in support of the global growth of fish production. Therefore, improvements in diet formulation are necessary to achieve the optimal requirements and attain a desirable growth efficiency and health performance in fish. Seven weeks of study were conducted to compare the equal amounts of different fatty acids (2%) (oleic acid, stearic acid, palmitic acid, and behenic acid) on the survival, the growth, and the immune response of hybrid grouper (Epinephelus fuscoguttatus × Epinephelus lanceolatus) against V. vulnificus. After six weeks of the feeding trial, fish were challenged with V. vulnificus for 30 min before continuing on the same feeding regime for the next seven days (post-bacterial challenge). Fish supplemented with dietary oleic acid showed significantly (p < 0.05) enhanced immune responses, i.e., lysozyme, respiratory burst, and phagocytic activities compared to the control diet group for both pre-and post-bacterial challenges. Following the Vibrio challenge, no significant effects of supplemented fatty acid diets on survival rate were observed, although dietary oleic acid demonstrated the highest 63.3% survival rate compared to only 43.3% of the control diet group. In addition, there were no significant effects (p > 0.05) on specific growth rate (SGR), white blood cell (WBC), and red blood cell (RBC) counts among all experimental diets. The results from this study suggest that among the tested dietary fatty acids, the oleic acid diet showed promising results in the form of elevated immune responses and increased disease resistance of the hybrid grouper fingerlings challenged with V. vulnificus. [ABSTRACT FROM AUTHOR]

This review mainly aimed to introduce the findings of research projects comparing the responses of tropical and temperate indigenes to heat. From a questionnaire survey on thermal sensation and comfort of Indonesians and Japanese, we found that the thermal descriptor "cool" in tropical indigenes connotes a thermally comfortable feeling, suggesting that linguistic heat acclimatization exists on a cognitive level. Ten male students born and raised in Malaysia were invited to Fukuoka, Japan, and compared their responses with 10 Japanese male students with matched physical fitness and morphological characteristics. Cutaneous thermal sensitivity: The sensitivities were measured at 28 °C. The forehead warm sensitivity was significantly blunted in Malaysians. The less sensitivity to the warmth of tropical indigenes is advantageous in respect to withstanding heat stress with less discomfort and a greater ability to work in hot climates. Passive heat stress: Thermoregulatory responses, especially sweating, were investigated, during the lower leg hot bathing (42 °C for 60 min). The rectal temperature at rest was higher in Malaysians and increased smaller during immersion. There was no significant difference in the total amount of sweating between the two groups, while the local sweating on the forehead and thighs was lesser in Malaysians, suggesting distribution of sweating was different from Japanese. Exercise: Malaysian showed a significantly smaller increase in their rectal temperature during 55% maximal exercise for 60 min in heat (32 °C 70% relative humidity), even with a similar sweating and skin blood flow response in Japanese. The better heat tolerance in Malaysians could be explained by the greater convective heat transfer from the body core to the skin due to the greater core-to-skin temperature gradient. In addition, when they were hydrated, Malaysian participants showed better body fluid regulation with smaller reduction in plasma volume at the end of the exercise compared to the non-hydrated condition, whereas Japanese showed no difference between hydration conditions. We further investigated the de-acclimatization of heat adaptation by longitudinal observation on the heat tolerance of international students who had moved from tropical areas to Fukuoka for several years. [ABSTRACT FROM AUTHOR]

Plasmodium knowlesi poses a health threat throughout Southeast Asian communities and currently causes most cases of malaria in Malaysia. This zoonotic parasite species has been studied in Macaca mulatta (rhesus monkeys) as a model for severe malarial infections, chronicity, and antigenic variation. The phenomenon of Plasmodium antigenic variation was first recognized during rhesus monkey infections. Plasmodium -encoded variant proteins were first discovered in this species and found to be expressed at the surface of infected erythrocytes, and then named the Schizont-Infected Cell Agglutination (SICA) antigens. SICA expression was shown to be spleen dependent, as SICA expression is lost after P. knowlesi is passaged in splenectomized rhesus. Here we present data from longitudinal P. knowlesi infections in rhesus with the most comprehensive analysis to date of clinical parameters and infected red blood cell sequestration in the vasculature of tissues from 22 organs. Based on the histopathological analysis of 22 tissue types from 11 rhesus monkeys, we show a comparative distribution of parasitized erythrocytes and the degree of margination of the infected erythrocytes with the endothelium. Interestingly, there was a significantly higher burden of parasites in the gastrointestinal tissues, and extensive margination of the parasites along the endothelium, which may help explain gastrointestinal symptoms frequently reported by patients with P. knowlesi malarial infections. Moreover, this margination was not observed in splenectomized rhesus that were infected with parasites not expressing the SICA proteins. This work provides data that directly supports the view that a subpopulation of P. knowlesi parasites cytoadheres and sequesters, likely via SICA variant antigens acting as ligands. This process is akin to the cytoadhesive function of the related variant antigen proteins, namely Erythrocyte Membrane Protein-1, expressed by Plasmodium falciparum. [ABSTRACT FROM AUTHOR]

Mitragyna speciosa, a species of plant that is native to Thailand, Malaysia and Southeast Asia, contains two major psychoactive alkaloids: mitragynine and 7-hydroxymitragynine. Pharmacologically, the alkaloids exhibit biphasic effects—at low doses, stimulant effects are realized, while high doses exhibit sedative effects. For years, the plant has been used recreationally and medicinally for these effects, but its use has been implicated in and associated with intoxications and deaths. In this case report, we describe two cases whereby decedents presented with single-substance fatal intoxications by mitragynine in the absence of other postmortem toxicological findings. The cases entail young male decedents in outdoor settings (e.g. driving a vehicle and bicycle). Postmortem blood concentrations were 2,325 and 3,809 ng/mL. The medical examiner certified the cause of death as acute mitragynine intoxication in both cases. The toxicology results presented become useful when considering mitragynine to be the offending agent in lethal single-drug intoxications; further, the information included is pertinent to medical examiners, forensic pathologists, forensic toxicologists and emergency department personnel in evaluating possible poisoning and lethality by mitragynine. [ABSTRACT FROM AUTHOR]

The secondary metabolites of endemic plants from the Rutaceae family, such as Burkillanthusmalaccensis (Ridl.) Swingle from the rainforest of Malaysia, has not been studied. Burkillanthusmalaccensis (Ridl.) Swingle may produce antibacterial and antibiotic-potentiating secondary metabolites. Hexane, chloroform, and methanol extracts of leaves, bark, wood, pericarps, and endocarps were tested against bacteria by broth microdilution assay and their antibiotic-potentiating activities. Chromatographic separations of hexane extracts of seeds were conducted to investigate effective phytochemicals and their antibacterial activities. Molecular docking studies of werneria chromene and dihydroxyacidissiminol against SARS-CoV-2 virus infection were conducted using AutoDock Vina. The methanol extract of bark inhibited the growth of Staphylococcusaureus, Escherichiacoli, and Pseudomonasaeruginosa with the minimum inhibitory concentration of 250, 500, and 250 µg/mL, respectively. The chloroform extract of endocarps potentiated the activity of imipenem against imipenem-resistant Acinetobacterbaumannii. The hexane extract of seeds increased the sensitivity of P. aeruginosa against ciprofloxacin and levofloxacin. The hexane extract of seeds and chloroform extract of endocarps were chromatographed, yielding werneria chromene and dihydroxyacidissiminol. Werneria chromene was bacteriostatic for P.aeruginosa and P.putida, with MIC/MBC values of 1000 > 1000 µg/mL. Dihydroxyacidissiminol showed the predicted binding energies of −8.1, −7.6, −7.0, and −7.5 kcal/mol with cathepsin L, nsp13 helicase, SARS-CoV-2 main protease, and SARS-CoV-2 spike protein receptor-binding domain S-RBD. Burkillanthusmalaccensis (Ridl.) Swingle can be a potential source of natural products with antibiotic-potentiating activity and that are anti-SARS-CoV-2. [ABSTRACT FROM AUTHOR]

1. Growth analysis based on tree-ring chronology is difficult in trees in aseasonal tropical rain forests, because annual growth rings may be unclear or completely absent. Fortunately, tree growth history recorded in xylem tissue is capable of providing valuable information on the responses of trees and forests to past and present environmental changes, including global warming. 2. We have developed a new technique for aseasonal tropical forest trees which derives their growth rates from xylem Δ14C, and verified its accuracy. We also determined, from xylem δ13C, the intrinsic water-use efficiency (iWUE) in the past 50 years. We analysed changes in xylem Δ14C and δ13C in 23 canopy trees of 12 species in 6 families growing in Pasoh Forest Reserve, Malaysia; each stem diameter at breast height (DBH) was recorded 14 times from 1969 to 2011. 3. We found a significant positive relationship between the growth rates determined by 14C dating and the past DBH data. On the other hand, leaf-internal CO2 (Ci) content did not change with increasing atmospheric CO2 (Ca). Thus, the iWUE increased significantly over the last 50 years in all the families and species tested. 4. This study showed that the simultaneous measurements of xylem Δ14C and δ13C could reveal a long-term change in tree growth and iWUE during the past 50 years with high accuracy in various species and/or individuals in aseasonal tropical rainforests exhibiting high species diversity. [ABSTRACT FROM AUTHOR]

This study aimed to estimate the potential waste management cost reduction and the recycling profit from the segregation programme implemented in Malaysia. The assessment was done among the states that implemented a waste segregation programme in Malaysia, a Southeast Asia country with a GDP of 364.7 billion USD. The solid waste (tonnes) data were collected from the Solid Waste Management and Public Cleansing Corporation, Malaysia, and analysed using mathematical models. On average, 2.69 million tonnes per year (t/yr) of waste were disposed of in the landfills, and 1,680 t/yr of waste was segregated, equivalent to 0.06%. Plastic (30.49%-39.48%) and paper (31.35%-40.88%) were the major components of segregated waste. Implementing the waste segregation program avoids the cost of waste disposal in landfills, potentially at 61,000 USD/yr and generating 130,000 USD/yr recycling profits. Therefore, the government should strengthen the programme to increase the segregation rate and provide a significant income to the community. [ABSTRACT FROM AUTHOR]

Copyright of Kranion is the property of Publicidad Permanyer SLU and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Asia currently faces a serious crisis involving plastic wastes. Efforts to curb plastic waste in the region are mostly hampered by inadequate recycling infrastructure, unsustainable disposal practice, low level of recycling awareness and persistent shipping of large amounts of waste from developed countries to the region. The ban on plastic waste import by China in 2017 worsened the situation by causing unsustainable shipping of waste from high plastic waste-generating countries such as Japan to alternate destinations in Southeast and East Asia. The Japanese government is frantically looking for options to manage plastic waste piling up on the domestic front. Malaysia became one of the leading alternative destinations for plastic waste after the ban by China. This work is aimed at conducting a comparative analysis of plastic recycling policies and legislations between Malaysia and Japan to yield synergistic solutions between the two countries to combat the current predicament. The comparison will signify two typical development patterns in developed countries and developing countries and will be able elucidate future directions for other countries with similar policy and legislative transitions in the region. A set of nine criteria was employed to critically evaluate the policies and legislations of both countries. Barriers faced by both countries in plastic recycling are outlined and recommendations are proposed to overcome those barriers on the domestic front. Two strategies proposed to be jointly developed by both countries are enhancement of extended producer responsibility (EPR) through a regional policy platform and collaboration in establishing joint Ecotowns. [ABSTRACT FROM AUTHOR]

Ketum use is significantly prevalent amongst individuals in the northern states of Peninsular Malaysia. This study aims to investigate the prevalence and psychosocial correlates of Ketum use in individuals who are in the Methadone Maintenance Therapy (MMT) Programme at the Hospital Taiping. This is a cross-sectional study conducted in the methadone clinic at the Hospital Taiping. The study instruments used were Subjective Opiate Withdrawal Scale (SOWS), Alcohol, Smoking and Substance Involvement Screening Test (ASSIST) questionnaire, and Kratom Dependence Scale (KDS). A total of 215 subjects were recruited for this study. The prevalence of ketum users was 49.3% (n = 106). Chinese and Indian ethnicity had a lower tendency to use ketum compared to Malay ethnicity, with OR = 0.386 (95% CI 0.134, 1.113) and 0.119 (95% CI 0.035, 0.408), respectively. Individuals who used other illicit drugs had a higher tendency to use ketum with the adjusted OR = 9.914 (95% CI: 1.109, 88.602). Every one unit increase in SOWS increased the odds of being a ketum user by 1.340 (95% CI: 1.070, 1.677), whereas every one unit increase in duration in the MMT programme reduced the odds of being a ketum user by 0.990 (95% CI: 0.982, 0.998). Ketum use is prevalent amongst those in the MMT programme in this study. The high prevalence of ketum use is of concern and further interventions should be carried out to address this. [ABSTRACT FROM AUTHOR]

Growth and photosynthetic response of four indigenous tree seedlings, i.e. Dyera costulata, Dipterocarpus baudii, Neobalanocarpus heimii and Gonystylus affinis were studied under different light conditions in a degraded secondary forest. Maximum photosynthesis (Amax) was measured at 2 and 12 months after planting. The ratio of variable to maximum fluorescence (Fv/Fm) was determined. Leaves measured at 2 and 12 months after planting were old leaves present on the seedlings and new leaves that had expanded after planting respectively. Seedling growth was measured over four years. Changes in the growth rate and Amax with canopy openness were categorised into two groups. The growth and Amax of the first group (D. costulata and D. baudii) were maximum at 30-40% canopy openness. This group may be suitable for planting under large canopy gaps. The second group (G. affinis and N. heimii) showed maximum growth and Amax at relatively low canopy openness (less than 20%). Leaves in the second group suffered chronic photoinhibition under large gap. These species were suitable to be planted under low light conditions. [ABSTRACT FROM AUTHOR]

Abstract  Natives of the tropics are able to tolerate high ambient temperatures. This results from their long-term residence in hot and often humid tropical climates. This study was designed to compare the peripheral mechanisms of thermal sweating in tropical natives with that of their temperate counterparts. Fifty-five healthy male subjects including 20 native Koreans who live in the temperate Korean climate (Temperate-N) and 35 native tropical Malaysian men that have lived all of their lives in Malaysia (Tropical-N) were enrolled in this study after providing written informed consent to participate. Quantitative sudomotor axon reflex testing after iontophoresis (2 mA for 5 min) with 10% acetylcholine (ACh) was used to determine directly activated (DIR) and axon reflex-mediated (AXR) sweating during ACh iontophoresis. The sweat rate, activated sweat gland density, sweat gland output per single gland activated, and oral and skin temperature changes were measured. The sweat onset time of AXR (nicotinic-receptor-mediated) was 56 s shorter in the Temperate-N than in the Tropical-N subjects (P P P P  [ABSTRACT FROM AUTHOR]

Background: This study analysed the multi-temporal trend in land cover, and modelled a future scenario of land cover for the year 2030 in the highly urbanized state of Selangor, Malaysia. The study used a Decision Forest-Markov chain model in the land change modeller (LCM) tool of TerrSet software. Land cover maps of 1999, 2006 and 2017 were classified into 5 classes, namely water, natural vegetation, agriculture, built-up land and cleared land. A simulated land cover map of 2017 was validated against the actual land cover map 2017. The Area Under the Curve (AUC) value of 0.84 of Total Operating Characteristics (TOC) and higher percentage of components of agreement (Hits + Correct rejection) compared to components of disagreement (Misses + False alarm + Wrong hits) indicated successful validation of the model. Results: The results showed between the years 1999 to 2017 there was an increase in built-up land cover of 608.8 km2 (7.5%), and agricultural land 285.5 km2 (3.5%), whereas natural vegetation decreased by 831.8 km2 (10.2%). The simulated land cover map of 2030 showed a continuation of this trend, where built-up area is estimated to increase by 723 km2 (8.9%), and agricultural land is estimated to increase by 57.2 km2 (0.7%), leading to a decrease of natural vegetation by 663.9 km2 (8.1%) for the period 2017 to 2030. The spatial trend of land cover change shows built-up areas mostly located in central Selangor where the highly urbanized and populated cities of Kuala Lumpur and Putrajaya and the Klang valley are located. Conclusion: The future land cover modelling indicates that built-up expansion mostly takes place at edges of existing urban boundaries. The results of this study can be used by policy makers, urban planners and other stakeholders for future decision making and city planning. [ABSTRACT FROM AUTHOR]

The study of processes that enrich positive relationships has been an under-researched area within positive psychology practice. The way an individual responds during couple conflicts (accommodation response) and toward the disclosure of good news of a partner (capitalization response) has been linked to relationship quality. Although the accommodation and capitalization communication processes are part and parcel of our everyday lives, the two processes have been examined separately and dominated by the Western perspectives in past research. Prior work has suggested that Western and Asian cultures differ in expressing and perceiving beneficial communication behaviors. Yet, it is still unclear which accommodation and capitalization responses matter the most from an Asian lens. To date, there is no research examining these interconnected variables simultaneously in Asia, specifically in Malaysia. In this study, two forms of communication processes, namely, (1) accommodation and (2) capitalization, were explored concurrently to disentangle the unique associations and influence on relationship satisfaction. This study also sought to understand the moderating effects of culture in terms of interdependent self-construal on the link between these two communication processes and relationship satisfaction. Responses of 139 Malaysians in dating relationships between the age of 18 and 30 years (M age = 23.15) were collected through online surveys. An active and constructive reaction was captured as the most favorable response through both the capitalization and accommodation processes. Prominently, an active-constructive capitalization response bore the strongest influence on relationship satisfaction above and beyond other responses. A passive and constructive response was revealed only fruitful for disclosures of positive news and not during conflicts. Conversely, in the destructive paradigm, passive-destructive responses were the most detrimental factor in relationships compared to other destructive responses. The results also uncovered that interdependent self-construal did not moderate the two forms of communication processes. However, the findings discovered unexpected individual and cultural variations. This pioneering study is a noteworthy addition to the positive psychology literature from an Asian standpoint. It highlights the significance of not only protecting relationships through better conflict management but also enriching relationships by capitalizing on the positive aspects across the lives of the couple, ultimately providing a greater holistic insight into cultivating flourishing lives. [ABSTRACT FROM AUTHOR]

While culture and depression influence the way in which humans process emotion, these two areas of investigation are rarely combined. Therefore, the aim of this study was to investigate the difference in facial emotion recognition among Malaysian Malays and Australians with a European heritage with and without depression. A total of 88 participants took part in this study (Malays n = 47, Australians n = 41). All participants were screened using The Structured Clinical Interview for DSM-5 Clinician Version (SCID-5-CV) to assess the Major Depressive Disorder (MDD) diagnosis and they also completed the Beck Depression Inventory (BDI). This study consisted of the facial emotion recognition (FER) task whereby the participants were asked to look at facial images and determine the emotion depicted by each of the facial expressions. It was found that depression status and cultural group did not significantly influence overall FER accuracy. Malaysian participants without MDD and Australian participants with MDD performed quicker as compared to Australian participants without MDD on the FER task. Also, Malaysian participants more accurately recognized fear as compared to Australian participants. Future studies can focus on the extent of the influence and other aspects of culture and participant condition on facial emotion recognition. [ABSTRACT FROM AUTHOR]

Background: Resistance to clarithromycin and levofloxacin in Helicobacter pylori which resulted in treatment failures has become a major challenge for physicians worldwide. The resistance is mainly mediated by mutations in a specific domain of the 23S rRNA, gyrA and gyrB genes for clarithromycin and levofloxacin respectively. Hence in this study, we aimed to investigate the current status of H. pylori resistance in our hospital to these two antibiotics based on the molecular approach. Materials and Methods: Gastric biopsy samples were obtained from treatmentnaïve patients. Bacterial genomic DNA was extracted using a commercial kit and continued with DNA amplification using polymerase chain reaction (PCR) with specific primers. The PCR amplicons were subjected to sequencing on 23S rRNA gene targeting nucleotide positions at 2,146, 2,147, 2,186 and amino acids at gyrA positions 87 and 91 and gyrB positions 436, 438, 481, 484 to investigate the possible mutations or polymorphisms of genes that lead to clarithromycin and levofloxacin resistance respectively. Results: Sixty-one urease-positive gastric biopsy samples were studied. The findings revealed the primary resistance rates to clarithromycin was 14.8% and to levofloxacin was 3.3% in our current scenario based on detection of reported resistance-related mutations of A2147G and D91N in 23S rRNA and gyrA genes, respectively. Interestingly, we found a high rate of silent mutations of the gyrA codon 87Asn (32.8%, 20/61) and two polymorphisms of the gyrB D481E (16.4%, 10/61) and R484K (21.3%, 13/61). The role of these polymorphisms in gyrB remained to be elucidated whether the levels of levofloxacin resistance are related to the position/amino acid. Conclusion: The primary resistance rate of H. pylori to clarithromycin has increased compared to the previous report in Malaysia. Therefore, molecular screening could aid and is important for the selection of antibiotics for H. pylori eradication therapies. [ABSTRACT FROM AUTHOR]

Studies Related To The Element Of Humor In Teaching In Malaysia Are Getting More Attention Even Though The Level Of Implementation Is Relatively Low. Liao Y, Luo S, Tsai M (2020), An Exploration Of The Relationships Between Elementary School Teachers Humor Styles And Their Emotional Labor Stated That Humor Can Promote The Professional Development Of Teachers To Build Appropriate Programs In The Work Environment. The Findings Of The Study Showed That 95 % Stated That The Element Of Humor Can Increase The Concern And Needs Of Teachers In Working. Thus, The Practice Of The Element Of Humor Among Teachers Should Be Applied In Teaching And Learning In The Classroom. [ABSTRACT FROM AUTHOR]

Background: Malaysia is on track towards malaria elimination. However, several cases of malaria still occur in the country. Contributing factors and communal aspects have noteworthy effects on any malaria elimination activities. Thus, assessing the community's knowledge, attitudes and practices (KAP) towards malaria is essential. This study was performed to evaluate KAP regarding malaria among the indigenous people (i.e. Orang Asli) in Peninsular Malaysia. Methods: A household-based cross-sectional study was conducted in five remote villages (clusters) of Orang Asli located in the State of Kelantan, a central region of the country. Community members aged six years and above were interviewed. Demographic, socio-economic and KAP data on malaria were collected using a structured questionnaire and analysed using descriptive statistics. Results: Overall, 536 individuals from 208 households were interviewed. Household indoor residual spraying (IRS) coverage and bed net ownership were 100% and 89.2%, respectively. A majority of respondents used mosquito bed nets every night (95.1%), but only 50.2% were aware that bed nets were used to prevent malaria. Nevertheless, almost all of the respondents (97.9%) were aware that malaria is transmitted by mosquitoes. Regarding practice for managing malaria, the most common practice adopted by the respondents was seeking treatment at the health facilities (70.9%), followed by self-purchase of medication from a local shop (12.7%), seeking treatment from a traditional healer (10.5%) and self-healing (5.9%). Concerning potential zoonotic malaria, about half of the respondents (47.2%) reported seeing monkeys from their houses and 20.1% reported entering nearby forests within the last 6 months. Conclusion: This study found that most populations living in the villages have an acceptable level of knowledge and awareness about malaria. However, positive attitudes and practices concerning managing malaria require marked improvement. [ABSTRACT FROM AUTHOR]

Large vertebrates are rarely considered important drivers of conspecific negative density-dependent mortality (CNDD) in plants because they are generalist consumers. However, disturbances like trampling and nesting also cause plant mortality, and their impact on plant diversity depends on the spatial overlap between wildlife habitat preferences and plant species composition. We studied the impact of native wildlife on a hyperdiverse tree community in Malaysia. Pigs (Sus scrofa) are abnormally abundant at the site due to food subsidies in nearby farmland and they construct birthing nests using hundreds of tree saplings. We tagged 34 950 tree saplings in a 25 ha plot during an initial census and assessed the source mortality by recovering tree tags from pig nests (n = 1672 pig-induced deaths). At the stand scale, pigs nested in flat dry habitats, and at the local neighbourhood scale, they nested within clumps of saplings, both of which are intuitive for safe and efficient nest building. At the stand scale, flat dry habitats contained higher sapling densities and higher proportions of common species, so pig nesting increased the weighted average species evenness across habitats. At the neighbourhood scale, pig-induced sapling mortality was associated with higher heterospecific and especially conspecific sapling densities. Tree species have clumped distributions due to dispersal limitation and habitat filtering, so pig disturbances in sapling clumps indirectly caused CNDD. As a result, Pielou species evenness in 400 m2 quadrats increased 105% more in areas with pig-induced deaths than areas without disturbances. Wildlife induced CNDD and this supported tree species evenness, but they also drove a 62% decline in sapling densities from 1996 to 2010, which is unsustainable. We suspect pig nesting is an important feature shaping tree composition throughout the region. [ABSTRACT FROM AUTHOR]

Background: This study analysed the multi-temporal trend in land cover, and modelled a future scenario of land cover for the year 2030 in the highly urbanized state of Selangor, Malaysia. The study used a Decision Forest-Markov chain model in the land change modeller (LCM) tool of TerrSet software. Land cover maps of 1999, 2006 and 2017 were classified into 5 classes, namely water, natural vegetation, agriculture, built-up land and cleared land. A simulated land cover map of 2017 was validated against the actual land cover map 2017. The Area Under the Curve (AUC) value of 0.84 of Total Operating Characteristics (TOC) and higher percentage of components of agreement (Hits + Correct rejection) compared to components of disagreement (Misses + False alarm + Wrong hits) indicated successful validation of the model. Results: The results showed between the years 1999 to 2017 there was an increase in built-up land cover of 608.8 km2 (7.5%), and agricultural land 285.5 km2 (3.5%), whereas natural vegetation decreased by 831.8 km2 (10.2%). The simulated land cover map of 2030 showed a continuation of this trend, where built-up area is estimated to increase by 723 km2 (8.9%), and agricultural land is estimated to increase by 57.2 km2 (0.7%), leading to a decrease of natural vegetation by 663.9 km2 (8.1%) for the period 2017 to 2030. The spatial trend of land cover change shows built-up areas mostly located in central Selangor where the highly urbanized and populated cities of Kuala Lumpur and Putrajaya and the Klang valley are located. Conclusion: The future land cover modelling indicates that built-up expansion mostly takes place at edges of existing urban boundaries. The results of this study can be used by policy makers, urban planners and other stakeholders for future decision making and city planning. [ABSTRACT FROM AUTHOR]

Objectives: To propose a novel robust radiogenomics approach to the identification of epidermal growth factor receptor (EGFR) mutations among patients with non-small cell lung cancer (NSCLC) using Betti numbers (BNs). Materials and methods: Contrast enhanced computed tomography (CT) images of 194 multi-racial NSCLC patients (79 EGFR mutants and 115 wildtypes) were collected from three different countries using 5 manufacturers' scanners with a variety of scanning parameters. Ninety-nine cases obtained from the University of Malaya Medical Centre (UMMC) in Malaysia were used for training and validation procedures. Forty-one cases collected from the Kyushu University Hospital (KUH) in Japan and fifty-four cases obtained from The Cancer Imaging Archive (TCIA) in America were used for a test procedure. Radiomic features were obtained from BN maps, which represent topologically invariant heterogeneous characteristics of lung cancer on CT images, by applying histogram- and texture-based feature computations. A BN-based signature was determined using support vector machine (SVM) models with the best combination of features that maximized a robustness index (RI) which defined a higher total area under receiver operating characteristics curves (AUCs) and lower difference of AUCs between the training and the validation. The SVM model was built using the signature and optimized in a five-fold cross validation. The BN-based model was compared to conventional original image (OI)- and wavelet-decomposition (WD)-based models with respect to the RI between the validation and the test. Results: The BN-based model showed a higher RI of 1.51 compared with the models based on the OI (RI: 1.33) and the WD (RI: 1.29). Conclusion: The proposed model showed higher robustness than the conventional models in the identification of EGFR mutations among NSCLC patients. The results suggested the robustness of the BN-based approach against variations in image scanner/scanning parameters. [ABSTRACT FROM AUTHOR]

Proboscis monkey (Nasalis larvatus) habitats throughout its range are becoming increasingly fragmented and degraded. Discerning patterns of abundance and distribution, and elucidating factors that affect the survival of this species in altered landscapes are vital for its conservation. We assessed population trends over 10 years in the Klias Peninsula of western Sabah, Malaysian Borneo. Our findings revealed that population size and distribution remained generally stable between 2004 and 2014, and little further habitat loss occurred over this interval. However, a positive correlation in proboscis monkey abundance was found with increasing proximity to areas where more large emergent trees remained along the riverbanks. This indicates that habitat quality is an ongoing conservation concern. Furthermore, we found that only about half of the available proboscis monkey habitat in this region is fully protected. The rest is within commercial and native-titled lands that are, by law, required to be converted to agriculture. For example, we identified key proboscis monkey habitat within and around the Padas Damit Class IV Amenity Forest Reserve, an area where we also found the highest densities of proboscis monkeys in the region. However, significant areas in close proximity or contiguous with this forest reserve have subsequently been converted to oil palm. Such development not only causes outright habitat loss but also risks disrupting connectivity of proboscis monkey habitat, especially along rivers. This in turn poses a threat not only to long-term viability of the local proboscis monkey population, but also to another vital aspect of the economy of the Klias region that depends on the draw of proboscis monkey-viewing for wildlife-based tourism. We suggest that mechanisms to safeguard the contiguity of remaining proboscis monkey habitat within this area should be implemented at the landscape level to ensure persistence of the local population. [ABSTRACT FROM AUTHOR]

The Oxyporus species from the Malay Peninsula are revised. The following two species are recognised: O. pendleburyi (= O. intermedius new synonym; O. atratus new synonym) and O. cameronianus, new species. All species are (re-)described with illustrations of the taxonomically important characters and photographs of their habitus. [ABSTRACT FROM AUTHOR]

The difficulty in differentiating the sex of monomorphic bird species has made molecular sexing an important tool in addressing this problem. This method uses noninvasively collected materials such as feathers and may be advantageous for sexing endangered as well as commercialized bird species. In this study, seven primer sets for sexing birds were screened in Aerodramus fuciphagus using a total of 13 feather samples that were randomly selected from the state of Perak, Malaysia. From the screening analysis, only one primer set (P8/WZ/W) successfully differentiated the sex of A. fuciphagus. PCR amplification produced a single 255‐bp DNA fragment for males which was derived from CHD‐Z (CHD gene region in the sex chromosome Z), while for the females it produced two fragments (144 and 255 bp). The 144‐bp fragment was from CHD‐W (CHD gene region in the sex chromosome W). Results from sequencing showed no variations in the base sequences of the CHD‐W and CHD‐Z amplified fragments within the same sexes, except for one male sample (A23) where at position 166, a base substitution occurred (G → A). Phylogenetic analysis of CHD‐W showed that four (Apodiformes; Gruiformes; Passeriformes; and Pelecaniformes) out of the five orders investigated had formed four clear clusters within their orders, including the studied order: Apodiformes. Whereas in CHD‐Z, four (Accipitriformes; Columbiformes; Galliformes; and Passeriformes) out of five orders investigated formed four clear clusters within their orders, excluding the studied order. In addition, A. fuciphagus and Apus apus (both Apodiformes) showed less divergence in CHD‐W than CHD‐Z (0% c.f. 9%). The result suggests that in A. fuciphagus, CHD gene evolution occurred at a higher rate in males (CHD‐Z) compared to females (CHD‐W). This finding may be useful for further studies on sex ratio and breeding management of A. fuciphagus. [ABSTRACT FROM AUTHOR]

Research Aims - This study investigates the influence of Japanese work cultures on Malaysian foodservice employees' work stress and their turnover intention. Design/methodology/approach - A total of 381 respondents participated in this study, and the research data was validated prior to the empirical assessment. Research Findings - The results of this study signify that the Japanese work culture amplifies workers disorientation, stress and turnover intention. Besides, thru hierarchical regression, workplace stress was found to mediate the relationship between Japanese work culture and employee turnover intention. Theoretical Contribution/Originality - The study reveals the conflicts between the South East Asian employees and the Japanese management culture in the foodservice industry. Managerial Implication in the South East Asian context - This study contributes to the organisational and human resource management literature by examining the direct and indirect effects of Japanese work culture on employees' workplace stress and their turnover intention in the context of the restaurant industry. Research limitation & implications - As for the study limitation, the current study does not cover all Japanese restaurant in Malaysia as the researchers focus on the Klang Valley setting. [ABSTRACT FROM AUTHOR]

EBV-associated gastric cancer (EBVaGC) is characterized by high frequency of DNA methylation. In this study, we investigated how epigenetic alteration of host genome contributes to pathogenesis of EBVaGC through the analysis of transcriptomic and epigenomic datasets from NIH TCGA (The Cancer Genome Atlas) consortium. We identified that immune related genes (IRGs) is a group of host genes preferentially silenced in EBV-positive gastric cancers through DNA hypermethylation. Further functional characterizations of selected IRGs reveal their novel antiviral activity against not only EBV but also KSHV. In particular, we showed that metallothionein-1 (MT1) and homeobox A (HOXA) gene clusters are down-regulated via EBV-driven DNA hypermethylation. Several MT1 isoforms suppress EBV lytic replication and release of progeny virions as well as KSHV lytic reactivation, suggesting functional redundancy of these genes. In addition, single HOXA10 isoform exerts antiviral activity against both EBV and KSHV. We also confirmed the antiviral effect of other dysregulated IRGs, such as IRAK2 and MAL, in scenario of EBV and KSHV lytic reactivation. Collectively, our results demonstrated that epigenetic silencing of IRGs is a viral strategy to escape immune surveillance and promote viral propagation, which is overall beneficial to viral oncogenesis of human gamma-herpesviruses (EBV and KSHV), considering that these IRGs possess antiviral activities against these oncoviruses. Author summary: Epstein-Barr virus (EBV), one of the human gamma-herpesviruses, is a well-defined viral agent that strongly associates with malignancies of lymphoid and epithelial origin. EBV-associated gastric carcinoma (EBVaGC) is the most common malignancy caused by EBV infection. In this paper, we identified that beyond tumor suppressor genes, immune related genes (IRGs) are another group of host genes that are extensively DNA hypermethylated and epigenetically silenced in EBVaGC comparing to non-EBV GC. For certain IRGs, such as metallothionein-1 (MT1) and homeobox A (HOXA) genes, the whole gene clusters undergo DNA hypermethylation in EBVaGC, while it also occurs for isolated individual IRGs, such as IRAK2 and MAL. Considering the critical role of innate immunity in antiviral control, we expected that silencing of IRGs would benefit EBV viral replication and propagation. Indeed, our further investigation of several IRGs (MT1G, HOXA10, IRAK2, and MAL) confirmed their antiviral activities against EBV lytic replication. Some of them also suppress lytic replication of another human gamma-herpesvirus, Kaposi's sarcoma-associated herpesvirus (KSHV), indicating their broad antiviral spectrum. Collectively, our results demonstrated that epigenetic silencing of antiviral IRGs is an efficient viral strategy utilized by oncoviruses, such as EBV, to escape immune surveillance and promote viral propagation, which is overall beneficial to viral oncogenesis. [ABSTRACT FROM AUTHOR]