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A key way to prevent undesirable fouling of any structure in the marine environment, without harming any microorganisms, is to use a polymer film with high hydrophobicity. The polymer film, which was simply prepared from a blend of hydrophobic polydimethylsiloxane elastomer and hydrophilic polyurethane, showed improved properties and economic viability for antifouling film for the marine industry. The field emission scanning electron microscope and energy dispersive X-ray spectrometer (FESEM and EDX) results from the polymer blend suggested a homogenous morphology and good distribution of the polyurethane disperse phase. The PDMS:PU blend (95:5) film gave a water contact angle of 103.4° ± 3.8° and the PDMS film gave a water contact angle of 109.5° ± 4.2°. Moreover, the PDMS:PU blend (95:5) film could also be modified with surface patterning by using soft lithography process to further increase the hydrophobicity. It was found that PDMS:PU blend (95:5) film with micro patterning from soft lithography process increased the contact angle to 128.8° ± 1.6°. The results from a field test in the Gulf of Thailand illustrated that the bonding strength between the barnacles and the PDMS:PU blend (95:5) film (0.07 MPa) were lower than the bonding strength between the barnacles and the carbon steel (1.16 MPa). The barnacles on the PDMS:PU blend (95:5) film were more easily removed from the surface. This indicated that the PDMS:PU blend (95:5) exhibited excellent antifouling properties and the results indicated that the PDMS:PU blend (95:5) film with micro patterning surface could be employed for antifouling application. [ABSTRACT FROM AUTHOR]