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Aim: The aim of the present study to compare the glucometer and laboratory glucose oxidase method for the estimation of blood glucose levels in neonates. Methods: The prospective analytical study was conducted in the Dept of pediatrics, Vardhman institute of medical sciences, Pawapuri, Nalanda, Bihar, India, for 12 months. 100 Patients were included in this study. The analysis was done on emergency basis within half an hour of collection of blood sample. Routine quality control check was followed for the analysis. A calibrated check using the manufacturer supplied control cuvette was performed once a day on the glucose analyser. Hypoglycemia was defined as blood glucose levels less than 45 mg/dl Hyperglycemia was defined as blood glucose levels more than 145 mg/dl. Blood glucose levels 45-145 mg/dl was considered as normal. Laboratory value of glucose was taken as gold standard. Results: 100 neonates were included in this study. Out of them 65 were males and 35 were females. 70 were term, 30 were preterm and majority being less than 7 days. Neonates were classified according to their gestational age by WHO Fenton charts. Maximum neonates (74%) were appropriate for gestational age ( AGA). Remaining 22 % were small for gestational age ( SGA) and 4 % were large for gestational age ( LGA). Glucometer had a specificity of 98%, sensitivity of 66%, positive predictive value of 96%, and negative predictive value of 85% and accuracy of 88%. Conclusion: The glucometer had good specificity and negative predictive value, confirmation with laboratory measurements of plasma glucose and clinical assessment of the infant are still of the utmost importance. [ABSTRACT FROM AUTHOR]