1. Immunoproteomics and immunoinformatics analysis of Cryptococcus gattii: novel candidate antigens for diagnosis.
- Author
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Martins LM, de Andrade HM, Vainstein MH, Wanke B, Schrank A, Balaguez CB, dos Santos PR, Santi L, Pires Sda F, da Silva AS, de Castro JA, Brandão RM, and do Monte SJ
- Subjects
- Antigens, Fungal genetics, Antigens, Fungal immunology, Cryptococcus gattii genetics, Electrophoresis, Gel, Two-Dimensional, Fungal Proteins genetics, Fungal Proteins immunology, Humans, Mass Spectrometry, Antigens, Fungal analysis, Cryptococcosis diagnosis, Cryptococcosis microbiology, Cryptococcus gattii chemistry, Cryptococcus gattii immunology, Fungal Proteins chemistry, Proteomics
- Abstract
Aim: To identify immunoreactive proteins of Cryptococcus gattii genotype VGII and their B-cell epitopes., Materials & Methods: We combined 2D gel electrophoresis, immunoblotting and mass spectrometry to identify immunoreactive proteins from four strains of C. gattii genotype VGII (CG01, CG02, CG03 and R265). Next, we screened the identified proteins to map B-cell epitopes., Results: Sixty-eight immunoreactive proteins were identified. The strains and the number of proteins we found were: CG01 (12), CG02 (12), CG03 (18) and R265 (26). In addition, we mapped 374 peptides potentially targeted by B cells., Conclusion: Both immunoreactive proteins and B-cell epitopes of C. gattii genotype VGII that were potentially targeted by a host humoral response were identified. Considering the evolutionary relevance of the identified proteins, we may speculate that they could be used as the initial targets for recombinant protein and peptide synthesis aimed at the development of immunodiagnostic tools for cryptococcosis.
- Published
- 2013
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