1. Effects of Se-depletion on glutathione peroxidase and selenoprotein W gene expression in the colon.
- Author
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Pagmantidis V, Bermano G, Villette S, Broom I, Arthur J, and Hesketh J
- Subjects
- Animals, Blotting, Northern, Caco-2 Cells, Colon enzymology, DNA, Complementary, Humans, Male, RNA, Messenger genetics, RNA, Messenger metabolism, Rats, Selenoprotein W, Selenoproteins, Colon metabolism, Glutathione Peroxidase genetics, Proteins genetics, Selenium metabolism
- Abstract
Selenium (Se)-containing proteins have important roles in protecting cells from oxidative damage. This work investigated the effects of Se-depletion on the expression of the genes encoding selenoproteins in colonic mucosa from rats fed diets of different Se content and in human intestinal Caco-2 cells grown in Se-adequate or Se-depleted culture medium. Se-depletion produced statistically significant (P<0.05) falls in glutathione peroxidase (GPX) 1 mRNA (60-83%) and selenoprotein W mRNA (73%) levels, a small but significant fall in GPX4 mRNA (17-25%) but no significant change in GPX2. The data show that SelW expression in the colon is highly sensitive to Se-depletion.
- Published
- 2005
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