1. CPEB3 regulates the proliferation and apoptosis of bovine cumulus cells
- Author
-
Zhe Zhang, Yan Gao, Cheng-Zhen Chen, Dong-Xu Han, Yan-Xia Peng, Bao Yuan, Fu Xuhuang, Ying Wang, Jia-Bao Zhang, Hao Jiang, Jian-Bo Liu, and Yi-jie Wang
- Subjects
Small interfering RNA ,Gene Expression ,Apoptosis ,03 medical and health sciences ,Downregulation and upregulation ,Gene expression ,CEBPB ,Animals ,RNA, Messenger ,030304 developmental biology ,Cell Proliferation ,Membrane Potential, Mitochondrial ,0303 health sciences ,Cyclin-dependent kinase 1 ,Cumulus Cells ,biology ,Chemistry ,Cell growth ,Cell Cycle ,0402 animal and dairy science ,Gene Expression Regulation, Developmental ,RNA-Binding Proteins ,04 agricultural and veterinary sciences ,General Medicine ,040201 dairy & animal science ,Cell biology ,biology.protein ,Cattle ,Female ,Cyclin-dependent kinase 6 ,General Agricultural and Biological Sciences ,Reactive Oxygen Species - Abstract
Cytoplasmic polyadenylation element-binding protein 3 (CPEB3) is a member of the Cytoplasmic polyadenylation element-binding family, which has been found to regulate the translation of dormant and masked mRNA in Xenopus oocytes and plays potential roles in regulating biological functions in cells and tissues. However, its role in cumulus cells is not clear. In this study, the mRNA expression of CPEB3 in bovine cumulus cells was inhibited with small interfering RNA. Cell cycle progression, proliferation, and apoptosis were measured after inhibition of CPEB3. Subsequently, changes in intracellular Reactive oxygen species content, mitochondrial membrane potential and expansion-related gene expression were examined. The results showed that after CPEB3 inhibition, cumulus cells had an abnormal cell cycle, the numbers of cells in the S and G2/M phases were significantly increased, cell proliferation was increased and apoptosis rates were decreased. These effects were likely due CPEB3 inhibition-induced decreases in intracellular Reactive oxygen species levels; increases in mitochondrial membrane potential; decreases in apoptosis; downregulation of CCNA, CCND, CCNE, CDK2, CDK4, CDK6, p21, and p27 mRNA expression; and upregulation of CCNB, CDK1, HAS2, PTGS2, PTX3, and CEBPB mRNA expression. Therefore, CPEB3 plays potential roles in regulating the biological and physiological functions of bovine cumulus cell.
- Published
- 2020