7 results on '"Fang LIU"'
Search Results
2. lncRNA profile of Apis mellifera and its possible role in behavioural transition from nurses to foragers
- Author
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Fang Liu, Lei Qi, Xin Su, Jie Dong, Zachary Y. Huang, Deqian Wang, and Tengfei Shi
- Subjects
0106 biological sciences ,lcsh:QH426-470 ,Odorant binding ,lcsh:Biotechnology ,mRNA ,Nurses ,Biology ,01 natural sciences ,03 medical and health sciences ,Exon ,lncRNA ,lcsh:TP248.13-248.65 ,Gene expression ,Genetics ,Animals ,RNA, Messenger ,030304 developmental biology ,0303 health sciences ,Messenger RNA ,Behavioural transition ,Behavior, Animal ,Sequence Analysis, RNA ,Gene Expression Profiling ,digestive, oral, and skin physiology ,fungi ,Foragers ,Wnt signaling pathway ,RNA ,Honey bee ,Bees ,lcsh:Genetics ,RNA, Long Noncoding ,DNA microarray ,010606 plant biology & botany ,Biotechnology ,Research Article - Abstract
Background The behavioural transition from nurses to foragers in honey bees is known to be affected by intrinsic and extrinsic factors, including colony demography, hormone levels, brain chemistry and structure, and gene expression in the brain. However, the molecular mechanism underlying this behavioural transition of honey bees is still obscure. Results Through RNA sequencing, we performed a comprehensive analysis of lncRNAs and mRNAs in honey bee nurses and foragers. Nurses and foragers from both typical colonies and single-cohort colonies were used to prepare six libraries to generate 49 to 100 million clear reads per sample. We obtained 6863 novel lncRNAs, 1480 differentially expressed lncRNAs between nurses and foragers, and 9308 mRNAs. Consistent with previous studies, lncRNAs showed features distinct from mRNAs, such as shorter lengths, lower exon numbers, and lower expression levels compared to mRNAs. Bioinformatic analysis showed that differentially expressed genes were mostly involved in the regulation of sensory-related events, such as olfactory receptor activity and odorant binding, and enriched Wnt and FoxO signaling pathways. Moreover, we found that lncRNAs TCONS_00356023, TCONS_00357367, TCONS_00159909 and mRNAs dop1, Kr-h1 and HR38 may play important roles in behavioural transition in honey bees. Conclusion This study characterized the expression profile of lncRNAs in nurses and foragers and provided a framework for further study of the role of lncRNAs in honey bee behavioural transition. Electronic supplementary material The online version of this article (10.1186/s12864-019-5664-7) contains supplementary material, which is available to authorized users.
- Published
- 2018
3. Characterization of long noncoding RNA and messenger RNA signatures in melanoma tumorigenesis and metastasis
- Author
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Ping Han, Feng Jin, Wenliang Fan, Siqi Wang, Fang Liu, Haibo Xu, Mengqi Tu, and Bing Wan
- Subjects
0301 basic medicine ,Melanomas ,Skin Neoplasms ,Microarrays ,Carcinogenesis ,Gene Expression ,lcsh:Medicine ,Bioinformatics ,Biochemistry ,Metastasis ,Database and Informatics Methods ,Basic Cancer Research ,Databases, Genetic ,Medicine and Health Sciences ,Neoplasm Metastasis ,lcsh:Science ,Melanoma ,Skin ,Regulation of gene expression ,Multidisciplinary ,Messenger RNA ,Genomics ,Genomic Databases ,Prognosis ,Long non-coding RNA ,Nucleic acids ,Gene Expression Regulation, Neoplastic ,Survival Rate ,Bioassays and Physiological Analysis ,Cell Transformation, Neoplastic ,Oncology ,RNA, Long Noncoding ,Research Article ,Biology ,Research and Analysis Methods ,03 medical and health sciences ,medicine ,Genetics ,Humans ,RNA, Messenger ,Non-coding RNA ,neoplasms ,Biology and life sciences ,Microarray analysis techniques ,Gene Expression Profiling ,lcsh:R ,Cancers and Neoplasms ,Computational Biology ,Correction ,medicine.disease ,Genome Analysis ,Gene expression profiling ,030104 developmental biology ,Biological Databases ,Cutaneous melanoma ,Cancer research ,Long non-coding RNAs ,RNA ,lcsh:Q ,Skin cancer - Abstract
The incidence of melanoma, the most aggressive and life-threatening form of skin cancer, has significantly risen over recent decades. Therefore, it is essential to identify the mechanisms that underlie melanoma tumorigenesis and metastasis and to explore novel and effective melanoma treatment strategies. Accumulating evidence s uggests that aberrantly expressed long noncoding RNAs (lncRNAs) have vital functions in multiple cancers. However, lncRNA functions in melanoma tumorigenesis and metastasis remain unclear. In this study, we investigated lncRNA and messenger RNA (mRNA) expression profiles in primary melanomas, metastatic melanomas and normal skin samples from the Gene Expression Omnibus database. We used GSE15605 as the training set (n = 74) and GSE7553 as the validation set (n = 58). In three comparisons (primary melanoma versus normal skin, metastatic melanoma versus normal skin, and metastatic melanoma versus primary melanoma), 178, 295 and 48 lncRNAs and 847, 1758, and 295 mRNAs were aberrantly expressed, respectively. We performed Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses to examine the differentially expressed mRNAs, and potential core lncRNAs were predicted by lncRNA-mRNA co-expression networks. Based on our results, 15 lncRNAs and 144 mRNAs were significantly associated with melanoma tumorigenesis and metastasis. A subsequent analysis suggested a critical role for a five-lncRNA signature during melanoma tumorigenesis and metastasis. Low expression of U47924.27 was significantly associated with decreased survival of patients with melanoma. To the best of our knowledge, this study is the first to explore the expression patterns of lncRNAs and mRNAs during melanoma tumorigenesis and metastasis by re-annotating microarray data from the Gene Expression Omnibus (GEO) microarray dataset. These findings reveal potential roles for lncRNAs during melanoma tumorigenesis and metastasis and provide a rich candidate reservoir for future studies.
- Published
- 2016
4. Rhubarb Tannins Extract Inhibits the Expression of Aquaporins 2 and 3 in Magnesium Sulphate-Induced Diarrhoea Model
- Author
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Na Lin, Chun-Fang Liu, Yan-Fang Zheng, Wen Xu, and Hui Wang
- Subjects
Diarrhea ,Article Subject ,medicine.drug_class ,Cell Survival ,Aquaporin ,Gene Expression ,lcsh:Medicine ,Biology ,General Biochemistry, Genetics and Molecular Biology ,HT29 Cells ,Magnesium Sulfate ,Mice ,In vivo ,Antidiarrhoeal ,medicine ,Animals ,Humans ,Protein kinase A ,Rheum ,Messenger RNA ,Aquaporin 3 ,Mice, Inbred ICR ,Aquaporin 2 ,General Immunology and Microbiology ,Plant Extracts ,lcsh:R ,General Medicine ,In vitro ,Disease Models, Animal ,Biochemistry ,Phosphorylation ,Tannins ,Research Article - Abstract
Tannins, a group of major active components of Chinese rhubarb and widely distributed in nature, have a significant antidiarrhoeal activity. Aquaporins (AQPs) 2 and 3 play important roles in regulating water transfer during diarrhoea. The present study aims to determine the effect of the total tannins extract of rhubarb on aquaporins (AQPs) 2 and 3 in diarrhoea mice and HT-29 cells both induced by magnesium sulphate (MgSO4). Our results showed that rhubarb tannins extract (RTE) significantly decreased the faecal water content in colon and evaluation index of defecation of diarrhoea mice. Interestingly, RTE could markedly reduce the mRNA and protein expression levels of AQPs 2 and 3 in apical and lateral mucosal epithelial cells in the colons of diarrhoea mice and HT-29 cells both induced by MgSO4in a dose-dependent manner. Furthermore, RTE suppressed the production of cyclic monophosphate- (cAMP-) dependent protein kinase A catalytic subunitsα(PKA C-α) and phosphorylated cAMP response element-binding protein (p-CREB, Ser133) in MgSO4-induced HT-29 cells. Our data showed for the first time that RTE inhibit AQPs 2 and 3 expressionin vivoandin vitrovia downregulating PKA/p-CREB signal pathway, which accounts for the antidiarrhoeal effect of RTE.
- Published
- 2014
5. Expression of plasma glutathione peroxidase in human liver in addition to kidney, heart, lung, and breast in humans and rodents
- Author
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James H. Doroshow, Xian-Fang Liu, Khiem Doan, Fong-Fong Chu, and R. S. Esworthy
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chemistry.chemical_classification ,medicine.medical_specialty ,Messenger RNA ,Kidney ,GPX3 ,Glutathione peroxidase ,Immunology ,Cell Biology ,Hematology ,Biology ,Biochemistry ,Molecular biology ,medicine.anatomical_structure ,Endocrinology ,chemistry ,Cell culture ,Placenta ,Internal medicine ,Complementary DNA ,Gene expression ,medicine - Abstract
We analyzed the expression of plasma glutathione peroxidase (GSHPx-P) messenger RNA (mRNA) in mouse, rat, and human tissues, using a human GSHPx-P cDNA clone as the probe. Unlike the classical cellular glutathione peroxidase (GSHPx-1), GSHPx-P expression appears to be tissue-specific. In the mouse and rat, kidney expresses an mRNA at a high level detected with the human probe. A signal is also detected in mRNA isolated from mouse and rat heart, rat cardiac myocytes, mouse lung, epididymis, and the mammary gland of midpregnant mice. No signal is detected in mRNA isolated from mouse and rat liver, mouse brain, uterus, and testis. In human tissues, an mRNA hybridizing to GSHPx-P cDNA is present in liver, as well as kidney, heart, lung, breast, and placenta. We have shown that human kidney expresses a GSHPx-P mRNA, and not a GSHPx-P-like message, by isolating a cDNA clone from a human kidney library in lambda gt11. From the 412-nucleotide partial sequence of the kidney cDNA, which codes for the 40–170 amino acids of GSHPx-P including the TGA codon for selenocysteine, we found complete sequence identity of the kidney cDNA with GSHPx-P isolated from placenta. The expression of GSHPx-P mRNA in cell lines was also studied. There is some correlation of the expression of GSHPx-P in these cell lines with that in normal tissues. Cell lines that expressed GSHPx-P mRNA or protein included the human hepatocarcinoma HepG2, Hep3B cells, human kidney carcinoma A498 cells, and the human breast cancer SK-BR-3, T47D, MDA-MB-231, and AdrrMCF-7 cells. Cell lines that did not express GSHPx- P included human choriocarcinoma BeWo cells, human breast cancer MCF-7, ZR-75–1, and Hs578T cells, and mouse hepatoma Hepa-1 cells.
- Published
- 1992
6. Identification and Characterization of a Novel Non-Coding RNA Involved in Sperm Maturation
- Author
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Yonglian Zhang, Li Zhang, Min-hua Lu, Qiang Liu, Mo-Fang Liu, Jinsong Zhang, Minjie Ni, and Zhi-Hong Hu
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Male ,Small RNA ,RNA, Untranslated ,Time Factors ,lcsh:Medicine ,Biochemistry ,MiRBase ,Rats, Sprague-Dawley ,Molecular cell biology ,Gene expression ,RNA Precursors ,Cloning, Molecular ,Small nucleolar RNA ,lcsh:Science ,Epididymis ,Genetics ,Multidisciplinary ,biology ,Chromosome Biology ,Physics ,Animal Models ,Non-coding RNA ,Chromatin ,Nucleic acids ,Organ Specificity ,Cellular Types ,Research Article ,Molecular Sequence Data ,Biophysics ,Down-Regulation ,Real-Time Polymerase Chain Reaction ,Model Organisms ,microRNA ,Animals ,RNA, Messenger ,Biology ,Inflammation ,Messenger RNA ,Base Sequence ,Sequence Analysis, RNA ,lcsh:R ,RNA stability ,Clone Cells ,Rats ,Sperm Maturation ,Germ Cells ,RNA processing ,Gene Expression Regulation ,biology.protein ,RNA ,Rat ,lcsh:Q ,Sperm Capacitation ,Dicer - Abstract
A long and ever-expanding roster of small (∼20-30 nucleotides) RNAs has emerged during the last decade, and most can be subsumed under the three main headings of microRNAs (miRNAs), Piwi-interacting RNAs (piRNAs), and short interfering RNAs (siRNAs). Among the three categories, miRNAs is the most quickly expanded group. The most recent number of identified miRNAs is 16,772 (Sanger miRbase, April 2011). However, there are insufficient publications on their primary forms, and no tissue-specific small RNAs precursors have been reported in the epididymis. Here, we report the identification in rats of an epididymis-specific, chimeric, noncoding RNA that is spliced from two different chromosomes (chromosomes 5 and 19), which we named HongrES2. HongrES2 is a 1.6 kb mRNA-like precursor that gives rise to a new microRNA-like small RNA (mil-HongrES2) in rat epididymis. The generation of mil-HongrES2 is stimulated during epididymitis. An epididymis-specific carboxylesterase named CES7 had 100% cDNA sequence homology at the 3'end with HongrES2 and its protein product could be downregulated by HongrES2 via mil-HongrES2. This was confirmed in vivo by initiating mil-HongrES2 over-expression in rats and observing an effect on sperm capacitation.
- Published
- 2011
7. Effects of mRNA amplification on gene expression ratios in cDNA experiments estimated by analysis of variance.
- Author
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Nygaard, Vigdis, Løland, Anders, Holden, Marit, Langaas, Mette, Rue, Håvard, Fang Liu, Myklebost, Ola, Fodstad, Øystein, Hovig, Eivind, and Smith-Sørensen, Birgitte
- Subjects
MESSENGER RNA ,GENE expression ,DNA microarrays ,ANALYSIS of variance ,GENETIC regulation - Abstract
Background: A limiting factor of cDNA microarray technology is the need for a substantial amount of RNA per labeling reaction. Thus, 20-200 micro-grams total RNA or 0.5-2 micro-grams poly (A) RNA is typically required for monitoring gene expression. In addition, gene expression profiles from large, heterogeneous cell populations provide complex patterns from which biological data for the target cells may be difficult to extract. In this study, we chose to investigate a widely used mRNA amplification protocol that allows gene expression studies to be performed on samples with limited starting material. We present a quantitative study of the variation and noise present in our data set obtained from experiments with either amplified or non-amplified material. Results: Using analysis of variance (ANOVA) and multiple hypothesis testing, we estimated the impact of amplification on the preservation of gene expression ratios. Both methods showed that the gene expression ratios were not completely preserved between amplified and non-amplified material. We also compared the expression ratios between the two cell lines for the amplified material with expression ratios between the two cell lines for the non-amplified material for each gene. With the aid of multiple t-testing with a false discovery rate of 5%, we found that 10% of the genes investigated showed significantly different expression ratios. Conclusion: Although the ratios were not fully preserved, amplification may prove to be extremely useful with respect to characterizing low expressing genes. [ABSTRACT FROM AUTHOR]
- Published
- 2003
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