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The bus stop layout and route deployment may influence the efficiency of bus services. Evaluating the supply of bus service requires the consideration of demand from various urban activities, such as residential and job-related activities. Although various evaluation methods have been explored from different perspectives, it remains a challenging issue. This study proposes a spatial statistical approach by comparing the density of the potential demand and supply of bus services at bus stops. The potential demand takes jobs-housing locations into account, and the supply of bus services considers bus stops and their associated total number of daily bus arrivals. The kernel density estimation (KDE) and spatial autocorrelation analyses are employed to investigate the coupling relationship between the demand and supply densities at global and local scales. A coupling degree index (CDI) is constructed to standardize the measurement of demand-supply balance. A case study in Wuhan, China demonstrated that: (1) the spatial distribution of bus stops is reasonable at global level, (2) Seriously unbalanced locations for bus services have been detected at several stops. Related adjustments that can improve these defects are highly recommended. [ABSTRACT FROM AUTHOR]