1. Spectrofluorimetric and molecular docking investigation on the interaction of 6-azauridine, a pyrimidine nucleoside antimetabolite, with serum protein.
- Author
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Uppuluri, Kiran Babu, Ayaz Ahmed, Khan Behlol, Jothi, Arunachalam, and Veerappan, Anbazhagan
- Subjects
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URIDINE , *ANTIMETABOLITES , *MOLECULAR docking , *FLUORIMETRY , *PYRIMIDINE nucleosides , *BLOOD proteins - Abstract
6-azauridine (AzU), a pyrimidine nucleoside antimetabolite, was known to inhibit the growth of microorganism and transplantable tumors. The binding affinity of AzU to blood serum proteins might influence the effectiveness of the drug. Herein, we investigated the interaction of AzU with bovine serum albumin (BSA) under near physiological conditions. Fluorescence spectroscopy analysis revealed that AzU strongly quenched the intrinsic fluorescence of BSA through a static quenching procedure, and a non-radiation energy transfer occurred within the molecules. Thermodynamic parameter determined from the temperature dependent binding constant suggests that the interaction of AzU with BSA is governed by enthalpic forces with a negative entropic contribution. Displacement studies and protein/drug docking studies suggest that AzU binds non-competitively to both sub-domain IIA and sub-domain IIIA of BSA. The obtained results reveal that AzU can bind to BSA with considerable affinity even in the presence of coexisting drug and can be used along with other drugs. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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