1. Cationic polyacrylamides promote binding of cellulase and amylase.
- Author
-
Reye JT, Maxwell KE, and Banerjee S
- Subjects
- Cellulose metabolism, Protein Binding drug effects, Starch metabolism, Acrylic Resins pharmacology, Amylases metabolism, Cellulase metabolism
- Abstract
Cationic polyacrylamides (c-PAMs) increase the binding of cellulase to cellulose and of α-amylase to cornstarch. The c-PAMs increase the rate of hydrolysis of both substrates by about the same amount, which suggests that the increased binding is responsible for the enhanced rates. These results support our proposed mechanism where the c-PAM neutralizes the charge of the substrate through a "patching" mechanism, which reduces the charge repulsion between fiber and enzyme., (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Published
- 2011
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